KR100624103B1 - Plant oil and chemical compound having acaricidal activity - Google Patents

Abstract

The present invention relates to plant essential oils and acaricide compounds having acaricidal property in the stored food mite, more specifically, Rhaphanus sativus L., Sinapis alba L., Prunus Armenicaa ( Prunus armeniaca LINNE), Prunus persica , Schizonepeta tenuifolia , Ephedra sinica , Cuminum cyminum and Chaenomeles sinensis Essential oils extracted from at least one plant selected from the group consisting of: terpinene-4-ol, tuzone, carbacrol, cuminaldehyde, linarol, menthol, mentone, anetol, 1-phenylpiperazine And a 4-arylanisole compound.

The acaricide according to the present invention exhibits excellent acaricide for stored food mites compared to conventional synthetic acaricides.

Acaricides, stored food mites, acaricides, plant essential oils,

Description

PLANT OIL AND CHEMICAL COMPOUND HAVING ACARICIDAL ACTIVITY

The present invention relates to a plant extract having an excellent acaricide effect on the stored food mites and acaricides comprising an essential oil component isolated therefrom.

Tyrophagus putrescentiae are very small (0.5 mm or less), have a clear cream color and are slow to move, making it almost impossible to visually identify them. These stored food mites are known as stored cereal pests distributed in food storage or barn, grain storage or mobile station, hay and grain flour. As the stored food mite is found to be the third dominant species among house dust mites in Korean houses, as well as diseases caused by eating food contaminated with ticks, that is, severe enteritis or anaphylaxis, (Hughes, AM ; The mites of stores food and houses.Technical Bulletin of the ministry of Agriculture , Fisheries and Food 9 : 1-400. 1976), potential as a causative antigen causing various allergic diseases such as asthma and rhinitis, conjunctivitis, atopic dermatitis This has been increased and is being studied (Woodcock, AA and Cunnington, AM; The allergenic importance of house dust and storage mites in asthmatics in Brunnei, SEAsia . Clin Allergy , 10 : 609, 19810). Moreover, even when controlling the stored food mites, the mite carcasses and excreta itself can become allergens and cause diseases, so it is almost impossible to eliminate allergen support.

Preserved mite species are the most common of the stored mites, which cannot grow in the dry state and occur at temperatures of 20 ° C. and at least 13% moisture (Gulati, R., Mathur). , S; Effects of Eucalyptus and Mentha leaves and Curcuma rhizomes on rophagus putrescentiae (Schrank) (Acarina: Acaridae) in wheat.Experimental and applied Acarology 19 : 511-518, 1995; Sinha, RB; Role of Acarina in stored grain ecosystem. Recent Adv.Acarol . 1 : 263-273, 1979). In particular, they live in foods that are high in fat and protein, such as dried eggs, ham, cheese, and various types of nuts, and seeds feed on pears, reducing the content of iron and vitamin B complexes. Reduction, causing serious economic losses (Kranz, GW; Some mites injurious to farm-stored grain. J. Econ. Entomol . 48 : 754-755, 1955; Ismael Sanchez-Ramos and Pedro Castanera; Acaricidal activity of natural monoterpenes on Tyrophagus putrescentiae (Schrank), a mite of stored food.J. Stored Prod.Res . 37: 93-101, 2001). In addition, harmful grains and bacteria, such as Aspergillus spp. And Penicillium spp., Can be transported in storage grains in warm environments.

Recently, due to the extreme environmental change and global warming phenomenon, the number of people is rapidly increasing and the damage is also increased by changing the residential environment such as central heating and duplex structure.

Accordingly, as the acaricide for controlling house dust mites (House Dust Mite), such as a storage food one is very important to control mites, big foot dust mite (Dermatophagoides farinae) and vertical pattern dust mites (Dermatophaoides pteronyssinus), which is currently under development Stored food mites are unable to control. This is due to the stored food mites and house dust mites having different physiology and mechanisms.

Accordingly, Kwon et al. (2002) reported that methanol crude extracts of Hydrangea paniculata (tree hydrangea), which show acaricide activity in stored food mites, have no activity against the two house dust mites. (Kwon, JH; Acaricidal activity of butylidenephthalide identified from Cnidium officinale rhizome against Dermatophagoides pteronyssinus , Dermatophagoides farinae (Acari: pyroglyphidae ) and Tyrophagus putrescentiae (Acari: acaridae ) .MS Thewon , Seoul Korea University 2002, Suh National University 2002 .

Currently, smoke control using metyhylene bromide, which does not cause food deterioration, for the control of stored food mites, spraying organophosphate compounds such as pyrimiphos-methyl or diethyl m -tolua It relies on chemical methods such as the treatment of repellents such as diethyl m- toluamid and benzyl benzoate.

Among them, pirimiphos-methyl has been used as a means of suppressing stored food mites due to its low mammalian toxicity, and Mitchell et al. Have 60% of ticks inhabiting carpets and 50 mites inhabiting carpets. It was reported to the control% (Mitchell, EB, Wilkins, S., McCallum Deighton, J. and Platts-Mills, TAE; Reduction of house dust mite allergen levels in the home:. use of the acaricide, pirimiphos methyl Clin Allergy 15 : 234-240, 1985). However, repeated use of chemicals for tick control has resulted in the development of serious resistance, causing adverse effects on non-specific plants and bringing importance to the environment and human health.

Therefore, there is an urgent need to find a new type of eco-friendly, strong acaricide and non-toxic substances that can overcome the disadvantages of synthetic acaricides.

In response to these demands, many studies have been conducted to develop natural acaricides using plants that are used as drug therapy in folk medicine and fragrant aromatic substances (Miyazaki, Y., Yatagai, M. and Takaoka, M). Jpn Biometeor . 26 : 105-108, 1989; Watanabe, F., Tadaki, S., Takaoka, M., Ishino, S. and Morimoto, I .; Killing Activities of the volatiles emitted from essential oils for Dermatophagoides pteronyssinus, Dermatophagoides farinae and Tyrophagus putrescentiae Syoyakugaku Zasshi 43:... 163-168, 1989; Yatagai, M. and Morita, S .; Extractives from Yakusugi bogwood and their termicidal activity and growth regulation effects on plant seeds Mokuzai Gakkishi J Japan Wood Res. Soc . 37 : 345-351, 1991; Morita, S., Yatagai, M. and Ohira, T .; Antimite and antifungal activities of the hexane extractives from yakusugi bogwood.Journal of the Japan Wood Research Soc . 37 (4) : 352-357, 1991).

Accordingly, an object of the present invention is to provide an acaricide comprising an essential oil component which is separated from the plant extract and exhibits acaricidal activity against stored food mites.

In order to achieve the above object, the present invention

Rhaphanus sativus L., Sinapis alba L., Prunus armeniaca LINNE, Prunus persica , Schizonepeta tenifolia , Provided are acaricides comprising essential oils extracted from at least one plant selected from the group consisting of Ephedra sinica , Cuminum cyminum and Chaenomeles sinensis .

In addition, the present invention is terpineen-4-ol (terpinen-4-ol), tuzon (thujone), carvacrol (cuvacrol), cuminaldehyde (linalool) isolated from the essential oil components extracted from the plant At least one compound selected from the group consisting of menthol, menthol, menthone, anethole, 1-phenylpiperazine and 4-allyylanisole Provides acaricide containing.

Hereinafter, the present invention will be described in more detail.

The present inventors screened compounds exhibiting acaricidal activity against stored food mites among highly volatile plants and compounds showing acaricidal activity among stored food mites among conventional compounds, and identified plant essential oils and compounds having acaricidal properties.

The astringent plants screened in the present invention are Rhaphanus sativus LINNE ( seedless seed), Sinapis alba L. (seed of mustard), Prunus armeniaca LINNE (apricot seed), pruner Prunus persica (Peach seeds), Schizonepeta tenuifolia ( Splash outpost) ( Ephedra sinica ; ephedra stem), Cuminum cyminum (Cumin seeds) ) And Chaenomeles sinensis (quince fruit).

The essential oil component extracted from the above-mentioned plants is preferably carried out by the extraction or preparation method of the essential oils conventional in the art (Natural Chemical Research Method Woo Wonsik), for example, continuous steam distillation method, ultrasonic extraction method, reflux cooling extraction method and cold immersion method It is carried out by one method selected from among, preferably by the method of continuous steam distillation method.

Hereinafter, a brief description of an extraction method commonly used in this field is as follows.

First, the dried plants are ground to a predetermined size, and then injected into the extraction reactor.

The extraction solvent is then injected in a certain amount to the plant. Usable extraction solvent may be a single solvent or a mixed solvent thereof selected from the group consisting of water, a lower alcohol having 1 to 5 carbon atoms, and an organic solvent. Preferably, the lower alcohol may be methanol, ethanol or isopropanol, and as the organic solvent, hexane, ethyl acetate or acetone, which are low polar solvents, may be used.

At this time, the content of the extraction solvent may be appropriately adjusted according to the solvent used, preferably, 1 to 20 times by volume ratio with respect to the weight of the dry pulverized plant when using water and lower alcohol, preferably 3 To 10 times, and 0.5 to 2 times for organic solvents, preferably 0.6 to 1 times.

Next, the plant immersed in the solvent is subjected to an extraction process for 1 hour to 48 hours, preferably 5 hours to 24 hours at 0 to 150 ℃, preferably 10 to 100 ℃ to obtain the essential oil components contained in the plant Extract.

Subsequently, the solution in the reactor is filtered to separate the essential oil components contained in the solvent through the previous extraction step, and the extract is separated and dried, followed by a concentration under reduced pressure at a temperature of 0 to 40 ° C., preferably 0 to 20 ° C. do. At this time, the drying agent used in the drying step is used anhydrous magnesium sulfate, anhydrous calcium sulfate, anhydrous calcium sulfate or silica gel anhydrous widely used in this field, preferably magnesium sulfate anhydride.

Of the essential oil components separated through the above steps, the compound that confirmed the acaricide effect by measuring the acaricide activity is represented by the formula (1 to 10).

Among the compounds of Formulas 1 to 10, the compound of Formula 1 is terpinen-4-ol, Formula 2 is tujone, Formula 3 is carvacrol, Formula 4 is cuminaldehyde (cuminaldehyde), formula 5 is linalool, formula 6 is menthol, formula 7 is menthone, formula 8 is anethole, formula 9 is 1-phenylpiperazine And Formula 10 are 4-allylanisole.

Essential oils represented by Formulas 1 to 10 are stored food mites (Tyrophagus putrescentiae) Has a killing effect.

Accordingly, the present applicant, in order to determine the astringent effect of the essential oil component, after dissolving the essential oil component obtained by extracting the plant as described above with hexane in methanol, by using it to measure the astringent effect on the stored food tick, At the concentration of 1 mg / tube it was confirmed that all plants have a 100% acaricide effect (see Table 2).

In addition, by purchasing the essential oil components represented by the formula 1 to 10 to measure the acaricide effect on the stored food ticks for each component, all plants have a 100% acaricide effect at a concentration of 0.5 mg / tube It could be confirmed (see Table 3).

Therefore, the essential oil component extracted from various plants according to the present invention, represented by the formula (1) to 10 is preferably used as acaricides, especially acaricides used where the stored food ticks.

Acaricides containing the essential oil component according to the present invention may be a conventional treatment method of pesticides, such as coating treatment, dipping treatment and fumigation treatment, the application dose is also possible to apply the conventional pesticides.

Acaricides containing the essential oil components can be prepared and applied in various forms, can be prepared in the form of a liquid, emulsion, coating or granules by dissolving or dispersing in one or more solvents for coating and dipping treatment, for fumigation treatment It is optionally used in the form of smokers, fumigants, coatings, granules and solids in combination with extenders, for example liquid solvents, pressurized liquefied gases and / or solid carriers, optionally with surfactants.

In order to prepare such various types of preparations, solvents and additives conventionally used in the art can be used for essential oils extracted from plants according to the invention. For example, ethanol or water, which is harmless to the human body, may be prepared in a liquid form using a dispersion, and may be prepared in a solid form using a conventional excipient or the like.

In addition, the concentration of the active ingredient in the acaricide is used in an amount effective to control the stored food mites, the specific concentration at this time depends on the type of formulation and the method of application.

On the other hand, the essential oils represented by Formulas 1 to 10 according to the present invention can be preferably used as a substrate of the parent compound for a novel compound having an effect on various filling. For example, the parent compound may be a hydroxyl group (-OH), an aldehyde group (-COH), an alcohol group (-OH), an amide group (-CONH) or an aryl group through a chemical synthesis based on the structure of a known substance Substituted derivatives can be prepared using synthetic methods customary in the art, such as alkylation, etherification or esterification reactions, such as -C ₆ H ₅ ).

For example, monoterpene essential oil components with alcohol substituents can be reacted with an carboxylic acid or derivative thereof under an acid catalyst to produce esterified monoferpene compounds, which are well known in the art (Ralph J). Fessenden & Joan S. Fessenden; Organic Chemistry 2nd Ed., PWS Publisher, Willard Grant Press, 1979). It is apparent to those skilled in the art that as a pesticide newly synthesized as a parent nucleus compound, compounds having stronger acaricide activity against ticks can be obtained as manufactured and used as derivative compounds having less photolysis and lower fish toxicity than the original components.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

Example 1

A: Extraction of Plant Extracts

To extract essential oils from eight plants, Rhaphanus sativus L., Sinapis alba L., Prunus armeniaca LINNE, Prunus persica ), Schizonepeta tenuifolia , Ephedra sinica , Cuminum cyminum , and Chaenomeles sinensis , and then finely dried in the shade It was.

Inject 300 ml of distilled water into 300 g of 8 kinds of finely planted plants, add 200 ml of hexane to the other reactor connected thereto, and extract continuously at 100 ° C. for 6 hours in a continuous steam distillation apparatus (SDE, Nickerson & Likens apparatus). It was.

The obtained hexane extract was dried over anhydrous magnesium sulfate, concentrated under reduced pressure at 20 ° C. or lower, sealed and stored frozen.

B: Separation of Essential Oils from Plant Extracts

After extracting the essential oil components from various plants in Example 1 was performed GC / MS (HP6890, JMS-600W) to identify the compounds of the essential oil components. However, if the identification of the sample is difficult, it was identified by comparison with a known mass spectra library (The Wiley Registry of Mass Spectral Data, 6th ed.).

The column used was a column packed with silica, and the conditions are as follows.

Column Name: Silica Capillary Column (J & W Scientific, 60 × 0.25 mm, length × thickness)

Temperature: 50 ° C. (15 min.) ⇒ 200 ° C. (15 min.)

Heating rate: 2 ℃ / min

Mobile phase gas: Helium (rate: 0.8 ml / min)

At this time, the compounds identified from each plant are shown in Table 1 below.

Scientific name Separation compound Rhaphanus sativus L. Limonene, thiazole, anethole, α-cubebene, β-caryophyllene, 4-allylanisole, linolenic acid (linoleic acid) Sinapis alba L. 1-butene, 2-carene, limonene, linalool, terpinen-4-ol, β-myrcene -myrcene, phenanthrene Prunus armeniaca linne Benzyl alcohol, benzaldehyde, 1-butene, 2,5-dimethylaniline, tujone Prunus persica , Benzaldehyde, benzyl alcohol, benzoic acid, 2-carene, carvacrol, linalool, tujone Schizonepeta tenuifolia Limonene, menhone, menthol, isomenthone, piperitenone, pulegone, β-caryophyllene, and germanicene D ( germacrene D) Ephedra sinica p - Im men (p -cymene), limonene (limonene), γ- emitter pinene (γ-terpinene), α- teopi nolren (α-terpinolene), Lena play (linalool), menson (menthone), thymol (thymol) , Terpinen-4-ol (terpinen-4-ol), piperitenone (piperitenone), 1-phenylpiperazine (1-phenylpiperazine) Cuminum cyminum Cumin aldehyde (cuminaldehyde), 3- Karen (3-carene), chi fee propylene (caryophyllene), Equus acid (cumic acid), p - Im men (p -cymene), β- myrcene (β-myrcene), α ? -Phellandrene,? -Pinene,? -Pinene,? -Pinene, sabinene,? -Terpinene, thymol Chaenomeles sinensis Lena play (linalool), menson (menthone), Poole Gon (pulegone), iso menson (isomenthone), Karen 2- (2-carene), p - among men (p -cymene), emitter penen 4-ol (terpinen -4-ol), linoleic acid

According to Table 1, it can be seen that the essential oil components such as various monoterpenes and aromatic substances are contained in each plant through an extraction process.

Experimental Example 1 Acaricide Activity of Plant Extracts

A: Mite used for acaricide activity assay

Acaricide activity was carried out on one type of stored food mite ( Tyrophagus putrescentiae ). At this time, the stored food mites were bred in a breeding room maintained at a relative humidity of 75 ± 5% and a temperature of 25 ± 1 ° C. In a 12.5 × 10.5 × 5 cm 3 container, the feed and mixed vitamin (Ebioze ^TM ) were 1: 1. A suitable amount of saline was poured into the mixed feed and the bottom of a container having a volume of 17.5 × 17.5 × 17.5 cm 3, and placed in a feeding room. The stock mites were bred without exposure to acaricide.

B: acaricide activity assay

In order to assay the acaricide activity of the essential oil component extracted from the plant in Example 1 was carried out as follows.

In the present invention, since the morphological characteristics of the stored food mite have a microscopic structure, 1 mg of the sample per 2 ml microtube was set through several preliminary experiments.

The essential oil component obtained in Example 1 was dissolved in 40 µl of methanol for each concentration (1.0, 0.5, 0.25, 0.125, 0.062, 0.031 mg / tube), and treated with a colorless microtube (2 ml) to cover the tube wall and lid. After shaking several times up and down so that the material was evenly dispersed and buried, the solvent was volatilized for 1 hour in a freeze dryer. Twenty five mites were put in each tube, and assayed under the conditions of 25 ± 1 ° C.

At this time, the stored food ticks were observed under a microscope to select only those individuals having excellent activity with a fine brush. Assay results were examined under an optical microscope (20 times) after 24 hours of treatment, and subjects with no movement due to stimulation of appendages and torso with a fine brush were considered dead. All assays were repeated 5 or more times and a comparison between the means for the rate of fertilization was made using Scheffe's test (SAS Institute, 1996).

The same procedure was performed without using the plant essential oil, and the results obtained are shown in Table 2 below. In this case, the letters (a, b, c, d) of Table 2 indicate that the average is not statistically different ( P = 0.05, Sheffe test), and the insecticidal rate is represented by transforming the square root of the arc sine.

Scientific name (essential oil) Meat ratio (mean ± standard deviation,%) Treatment Concentration (mg / tube) 1.0 0.50 0.250 0.125 0.062 0.031 Rhaphanus sativus L. 100 ^a 100 ^a 85 ± 1.5 ^a 45 ± 2.3 ^b 15 ± 1.2 ^c 5 ± 0.5 ^c Sinapis alba L. 100 ^a 100 ^a 100 ^a 100 ^a 100 ^a 50 ± 2.5 ^b Prunus armeniaca linne 100 ^a 100 ^a 100 ^a 100 ^a 90 ± 2.1 ^b 25 ± 1.4 ^c Prunus persica 100 ^a 100 ^a 100 ^a 100 ^a 100 ^a 100 ^a Schizonepeta tenuifolia 100 ^a 95 ± 1.1 ^b 40 ± 2.8 ^c 20 ± 3.1 ^c 5 ± 1.2 ^c 0 ^d Ephedra sinica 100 ^a 95 ± 2.1 ^b 45 ± 0.7 ^c 10 ± 0.9 ^c 0 ^d 0 ^d Cuminum cyminum 100 ^a 100 ^a 100 ^a 100 ^a 100 ^a 100 ^a Chaenomeles sinensis 100 ^a 100 ^a 7.5 ± 0.5 ^c 0 ^d 0 ^d 0 ^d

According to Table 2, when the treatment at a concentration of 1.0 mg / tube all the essential oil components extracted from the eight kinds of plants presented in Example 1 shows a 100% abundance ratio, it can be seen that all of the plants presented in the present invention has an astringent effect there was.

Among them, Raphanus sativas showed 100% agitation rate at concentrations of 1.0 and 0.5 mg / tube, and more than 40% agitation rate at 0.125 mg / tube. Could know.

Cinapis alba showed a strong fertilization effect of 100% at concentrations above 0.062 mg / tube and a high rate of fertilization of over 50% even at low concentrations of 0.031 mg / tube.

Prunus Armeniaca also exhibited a 100% agicide rate when treated at concentrations of 1.0, 0.5, 0.250 and 0.125 mg / tube, and showed a high agitation rate over 90% even at a concentration of 0.062 mg / tube. At low concentrations, about 25% of the fertilization rate can be seen.

In addition, cuminum siminum and prunus persica showed a 100% agitation rate even at a minimum concentration of 0.031 mg / tube, the most acaricidal effect among the plant essential oils obtained in Example 1.

In addition, Scytonepeta tenifolia, Ephedra snica and Kenomeles sinensis exhibited a high abundance of 95% or higher in the farms of 1.0 and 0.5 mg / tube, but all treatments of 0.250 mg / tube showed less than 50%. Seemed. In particular, it was confirmed that kenomeles sinensis showed no agitation rate at concentrations lower than 0.250 mg / tube.

As shown in Table 2, the effective content of the various types of acaricides including the essential oil component of the plant may be properly adjusted through the ratio of the treatment according to the treatment concentration.

Experimental Example 2 Acaricide Activity of Compounds Derived from Plant Essential Oils

In order to determine the acaricide effect of the essential oil component identified in the extract of Example 1, the screening for the acaricide effect using a variety of essential oil components. As a result, it was confirmed that terpinene-4-ol, tuzon, carbacroll, cuminaldehyde, linarol, menthol, mentone, anetol, 1-phenylpiperazine and 4-allyl anisole have a potency against the stored food mite. It was. Thus, by performing the same method as in Experimental Example 1, the agitation rate of the essential oil components as described above was measured, and the results obtained are shown in Table 3 below. In this case, benzyl benzoate and dibutyl phthalate, which were known to have excellent salvation effects, were used as controls.

compound Salvage rate (mean ± standard deviation,%) Treatment Concentration (mg / tube) 1.0 0.50 0.250 0.125 0.062 Essential oil Terpinene-4-ol 100 ^a 100 ^a 100 ^a 90 ^b 10 ^c Tuzon 100 ^a 100 ^a 97.5 ± 1.7 ^b 15 ± 3.3 ^c 7.5 ± 3.9 ^c Kabakrole 100 ^a 100 ^a 100 ^a 100 ^a 87.5 ± 1.6 ^b Cuminaldehyde 100 ^a 100 ^a 100 ^a 97.5 ± 0.9 ^b 22.5 ± 2.1 ^c Linarol 100 ^a 100 ^a 100 ^a 90 ± 1.6 ^b 45 ± 1.4 ^c menthol 100 ^a 100 ^a 97.5 ± 2.0 ^b 100 ^a 35 ± 1.2 ^c Menson 100 ^a 100 ^a 100 ^a 97.5 ± 1.3 ^b 25 ± 0.8 ^c Anetol 100 ^a 100 ^a 100 ^a 17.5 ± 0.9 ^c 2.5 ± 0.8 ^c 1-phenylpiperazine 100 ^a 100 ^a 90 ± 1.0 ^b 95 ± 1.1 ^b 30 ± 0.7 ^c 4-allyl anisole 100 ^a 100 ^a 72.5 ± 2.6 ^b 12.5 ± 1.4 ^c 0 ^d Control Benzyl benzoate 100 ^a 100 ^a 100 ^a 32.0 ± 2.9 ^c 0 ^d Dibutyl phthalate 100 ^a 100 ^a 92.0 ± 3.8 ^b 62.2 ± 1.9 ^b 29.9 ± 1.7 ^c

Referring to Table 3, the compounds represented by Formulas 1 to 10 showed an excellent acaricide effect on the stored food ticks in each concentration range, especially 100% of the compound at the treatment level of 1.0 and 0.5 mg / tube concentration The rate of fat was shown.

At the 0.250 mg / tube concentration level, terpinene-4-ol, carbacroll, cuminaldehyde, linarol, mentone, and anetol still showed 100% salvage, while tuzone, menthol and 1-phenylpiperazine were 90%. More than% of fat

In particular, compared to the control of benzyl benzoate and dibutyl phthalate, which are known to have excellent acaricide effect, terpinene-4-ol, carbacrolol, cuminaldehyde, linarol, menthol, menson and All 1-phenylpiperazine showed an excellent agitation rate of more than 95%, and carbacrol showed a high agitation rate of more than 87.5% even at the minimum treatment concentration of 0.062 mg / tube.

As shown in Table 3, the effective content of the various types of acaricides including the compounds identified in the plant essential oils may be properly adjusted through the ratio of the treatment according to the treatment concentration.

As mentioned above, the essential oil component extracted from the plant of the present invention and the compound identified in the essential oil component exhibit an excellent astringent ability to store food ticks and has an excellent acaricide effect compared to the conventional synthetic acaricides.

Claims (6)

At least one plant extract selected from the group consisting of Rhaphanus sativus L., Schizonepeta tenuifolia , Ephedra sinica and Cuminum cyminum as an active ingredient Acaricides, characterized in that it has an acaricide effect against a stored food tick ( Tyrophagus putrescentiae ) containing. The acaricide according to claim 1, wherein the extract is selected and extracted from a single solvent selected from the group consisting of water, a lower alcohol having 1 to 5 carbon atoms, and an organic solvent, or a mixed solvent thereof. The acaricide according to claim 1, wherein the plant extract comprises a plant essential oil component. According to claim 3, wherein the plant essential oil components are tujone (thujone), cuminaldehyde (cuminaldehyde), linalool (linalool), menthol (menthol), menthol (menthone), anethol (alnethole) and 1-phenylpiperazine ( 1-phenylpiperazine) at least one compound selected from the group consisting of acaricide. delete The acaricide according to claim 1, wherein the acaricide is used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, fumigants, fumigants, granules and solid agents.