KR20220078055A - Composition and Method for Controling Mosquito Using an Extract of Cyperus rotundus - Google Patents

Abstract

The present invention discloses a composition for controlling mosquitoes using Hyangbuja extract having an insecticidal effect on mosquitoes and a method for controlling the same.

Description

Composition and Method for Controlling Mosquito Using an Extract of Cyperus rotundus

The present invention relates to a composition for controlling mosquitoes using an extract of Hyangbuja ( Cyperus rotundus ) and a method for controlling the same.

Mosquitoes, which interfere with sleep in summer and limit outdoor activities, are representative pests that transmit diseases to humans. About 3,500 species are known on Earth, and 55 species in 9 genera are known in Korea. Mosquitoes generally engage in blood-sucking activities to obtain animal protein in order to mature female adult eggs after mating, and secrete saliva containing pathogens or viruses at the blood-sucking site. Through this saliva, mosquitoes transmit diseases such as malaria, encephalitis, yellow fever, dengue fever, and filariasis (Science, 257:37-38, 1992). ), especially Japanese encephalitis, which occurs frequently in Korea, has a high mortality rate and is known to leave severe sequelae such as mental retardation even if treated.

Organophosphorus insecticides and pyrethroid insecticides have been used to control mosquitoes, but the most widely used material is diethyltoluamide (DEET). DEET was first developed by the US military in 1946 and has been used extensively to control various pests such as mosquitoes, flies, fleas, and ticks until now (Int. J. Pharm. 163:167-176, 1998). However, due to its high skin penetration, it can cause transdermal toxicity (Drug Metab. Dispos. 22:106-112, 1994) and cause diseases such as encephalopathy, acute manic psychosis, and cardiovascular disease. (Am. Mosq. Control Assoc. 11: 274-278, 1995). Therefore, its use is restricted for children, pregnant women, hypotensive patients, and people with sensitive skin, and in particular, in the United States, it is regulated not to manufacture mosquito repellents containing DEET at a concentration of 20% or more.

For these reasons, recent efforts have been made to develop mosquito control agents using natural products with relatively few side effects, such as Achyranthes bidentata extract, Cyanotis arachnoidea extract, etc. (Korean Patent No. 10-1778051), Neem oil, etc. (Korean Patent Publication No. 10-2017-0049822), Bay ( Pimenta racemosa ) extract, etc. (Korean Patent Publication No. 10-2004-0070903), Clove Oil, etc. (Korean Patent Publication) No. 10-2012-0014536), Hwangbyeok tree extract, etc. (Korean Patent No. 10-1470477), Citronella essential oil, etc. (Korean Patent Publication No. 10-2014-0042008) are natural product-derived mosquito repellents. has been proposed.

The present invention also discloses an extract of Hyangbuja having control activity against mosquitoes.

It is an object of the present invention to provide a composition for controlling mosquitoes using the Hyangbuja extract.

Another object of the present invention is to provide a method for controlling mosquitoes using the Hyangbuja extract.

Other objects or specific objects of the present invention will be set forth below.

As confirmed in the following Examples and Experimental Examples, the present invention is completed by confirming the insecticidal effect of the Hyangbuja extract against Aedes aegypti mosquitoes and Aedes rhododendrons.

Therefore, the composition for controlling mosquitoes of the present invention is characterized in that it contains the Hyangbuja extract as an active ingredient.

In the present specification, as used herein, the term "hyangbuja extract" refers to the extraction target of hyangbuja leaves, stems, above-ground parts, rhizomes, roots, underground parts, or mixtures thereof with water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.) , methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or these It means an extract obtained by leaching using a mixed solvent of In consideration of this, any method such as chilling, reflux, warming, ultrasonic radiation, or supercritical extraction may be applied. In the case of the fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and adsorbing the crude extract to a column filled with silica gel, etc. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent is removed by methods such as freeze drying, vacuum drying, hot air drying, spray drying, and the like. Preferably, it refers to an extract obtained by using water, ethanol, or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

Also, in the present specification, the term "active ingredient" means a component that can exhibit the desired activity alone or can exhibit activity together with a carrier having no activity by itself.

Also in this specification, "mosquito control" means mosquito repellent or mosquito repellent. Here, the mosquitoes are Aedes aegypti , Aedes albopictus , and other Aedes genus mosquitoes, Culex pipiens pallens , Culex tritaeniorhynchus, and Aedes mosquito ( Culex tritaeniorhynchus ). Culex pipiens molestus Forskal ), tropical house mosquito ( Culex quinquefasciatus ), mosquitoes of the genus Culex , such as Culex inatomii , Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito ( Anopheles gambiae ), It refers to mosquitoes of the Anopheles genus, such as Anopheles minimus and Anopheles stephensi .

In the composition of the present invention, the active ingredient may be included in any amount (effective amount) depending on the use, formulation, etc. as long as it can exhibit mosquito control activity, etc., and a typical effective amount is 0.001 wt % based on the total weight of the composition to 20.0% by weight. Here, "effective amount" refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit a mosquito control effect. Such an effective amount can be determined empirically within the ordinary ability of one of ordinary skill in the art.

In addition to the active ingredient, the composition of the present invention may include any compound or natural product-derived material known to have mosquito control activity in the art to enhance the mosquito control activity. Such compounds or substances derived from natural products are, for example, 1-(imidazolin-2-yl)amino-1,2-diphenylethane compound (Korean Patent Application No. 2007-7029894), 1-(1,2-diphenyl- Ethyl)-3-(2-hydroxyethyl)-thiourea compound (Korean Patent Application No. 2007-7029893), N-(4-pyridyl)methylsulfonamide (Korean Patent Application No. 2007-7023622), 2 -Cyano-3-(halo)alkoxy-benzenesulfonamide (Korean Patent Application No. 2007-7014451), hydrazide compound (Korean Patent Application No. 2007-7012272), 6-(2-chloro-5-halophenyl )-triazolopyrimidine (Korean Patent Application No. 2006-7014704), spiropiperidine derivative (Korean Patent Application No. 2006-7011568), nitrile compound (Korean Patent Application No. 2006-7011448), N-arylhydrazine derivatives (Korean Patent Application No. 2006-7010797), and the like, and DEET as previously exemplified, Achyranthes bidentata extract, Cyanotis arachnoidea extract, neem oil, Pimenta racemosa ) extract, clove oil, champignon extract, and citronella essential oil.

In general, vegetable oils are known to have pest repelling activity and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and any vegetable oils other than the oils exemplified above may be used as long as they have pest control activity. It may be included in the composition together with the active ingredient.

The composition of the present invention can be prepared in any dosage form according to the application method by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Specifically, it may be prepared in the form of oil, powder, suspension, fumigant, electric mat, ointment, cream, lotion, gel, external solution, pasta, liniment, soap, spray, and the like. Here, "pharmaceutically acceptable" means that it does not inhibit the activity of an active ingredient and does not have more toxicity than can be adapted to the human body. Regarding pharmaceutically acceptable carriers, reference may be made to Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995, which is incorporated herein by reference.

The carrier that can be used includes a solid carrier, a liquid carrier, a gaseous carrier, a surfactant, a dispersant, and the like. Examples of the solid carrier include talc, bentonite, clay, kaolin, diatomaceous earth, vermiculite, calcium carbonate, and the like, and a liquid carrier. As methanol, n-hexanol, ethylene glycol, acetone, methyl ethyl ketone, cyclohexanone, n-hexane, kerosine, kerosene, toluene, xylene, methyl naphthalene, diethyl ether, dioxane, tetrahydrofuran, ethyl acetate , acetonitrile, isobutyronitrile, dimethylformamide, dimethylacetamide, soybean oil, cottonseed oil, dimethyl sulfoxide, water, etc. may be exemplified, and as the gaseous carrier, LPG, air, nitrogen, carbon dioxide, dimethyl ether, etc. may be exemplified can

The control agent composition of the present invention may also include one or more adjuvants such as wetting agents, dispersing agents, surfactants, antifreezing agents, thickening agents, preservatives, spreading agents, and the like.

Such adjuvants may be appropriately selected from those known in the art. For example, alkylnaphthalene sulfonate (especially diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) may be used as a wetting agent, and a nonionic dispersing agent may be used. Dispersing agent (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersing agent (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonate, alkyl sulfonate) paint, alkylsulfonate, etc.) or mixtures thereof, and as the surfactant, alkylbenzenesulfonate, alkylnaphthalenesulfonate, dialkylsulfosuccinate, ligninsulfonate, alkylnaphthalenesulfonate formalin condensate, polyoxy sodium or calcium salts of sulfonates such as alkylenealkylphenylsulfonate, sodium or calcium salts of sulfates such as alkylsulfate, polyoxyalkylenealkylsulfate, polyoxyalkylenealkylphenylsulfate, naphthalenesulfosuccinate; Anionic surfactants such as sodium or calcium salts of succinate such as polyoxyalkylene succinate, ethoxylated alkyl ethers, polyoxyalkylene alkylphenyl polymers, polyalcohols, etc. can be used, and as antifreeze agents, methanol, ethanol , isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used. As a thickener, cellulose derivatives, polyacrylic acid derivatives, xanthan, modified clay, etc. can be used. As a preservative, dichlorophen, isothiazolene, benzyl alcohol, etc. As the electrodeposition agent, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. can be used. can

In another aspect, the present invention relates to a method for controlling mosquitoes using the composition for controlling mosquitoes of the present invention as described above.

The mosquito control method of the present invention comprises the step of contacting the mosquito control composition of the present invention as described above with mosquitoes.

The step of contacting the composition for controlling mosquitoes with pests may be accomplished by spraying or spraying the composition for controlling mosquitoes of the present invention at a place or location where mosquitoes inhabit or distribute, or by applying the composition to human skin where mosquitoes approach.

As described above, according to the present invention, it is possible to provide a composition and method for controlling mosquitoes using the Hyangbuja extract. The composition for controlling mosquitoes of the present invention may be sprayed or sprayed on a place or location where mosquitoes live or are distributed for mosquito control, or applied to human skin, etc. where mosquitoes approach.

1 is an experimental result showing the mosquito insecticidal activity of Hyangbuja extract.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

< Example > Preparation of Hyangbuja extract

Hyangbuja ( Cyperus rotundus ) 1L of fermented alcohol (95% ethanol) was added to 200 g of dry rhizome powder, and extracted by immersion for 5 days at room temperature. After filtering with Whatman paper filter paper No. 2, a yellow extract solution was obtained. .

< Experimental example > Mosquito insecticidal efficacy test

1. Experimental mosquito

Aedes albopictus and Culex pipiens used in the experiment were purchased from the Korea Centers for Disease Control and Prevention and bred under conditions of 26±1℃ and 60±5% relative humidity without exposure to pesticides. Larvae were reared in a plastic container (24 × 35 × 5 cm) containing 0.5 g of sterilized feed (40 mesh chick powder feed: yeast, 1:1 weight ratio). Adults were supplied with a 10% sugar solution and sucked blood to live mice for spawning.

Mosquitoes used for the test were those of the same age, 3 to 4 days after emergence from the pupa, and the test mosquitoes were supplied with sugar solution instead of blood until use, and were fasted for about 12 hours before the start of the test.

2. Insecticide effect test

2.1. Exam conditions

The insecticidal effect was investigated according to the Bottle Bioassay of the World Health Organization, Brogdon and McAllister (1998) and the U.S. Centers for Disease Control.

2.2. Test Methods

500 μL of liquid test substance was treated in a 500 mL glass bottle. The test substance was buried inside the bottle by shaking it well and rolling it so that the treatment solution could be uniformly applied to the wall. After drying in a hood for 2 hours to completely evaporate the solvent, 10 female adult mosquitoes were added each. All experiments were performed in 3 replicates. The control group was a test group that was not treated with the test substance (treated only with ethanol).

2.3. result evaluation

Mosquitoes in treated and untreated controls were left under light and dark conditions for 12:12 hours, 26 ± 1°C, and 60 ± 5% relative humidity, and mortality rates were investigated 24 hours after treatment.

3. Results - insecticidal effect

The mortality rates and average values of the results of three experiments for the adult Aedes Aedes mosquitoes and Aedes Aedes mosquitoes of the test substance are shown in Table 1 and FIG. 1 below.

Claims (6)

A composition for controlling mosquitoes comprising Hyangbuja extract as an active ingredient.
The method of claim 1,
The mosquitoes are Aedes genus mosquitoes, Culex genus Mosquitoes or Aedes genus Mosquitoes (Anopheles) Mosquitoes.
The method of claim 1,
The mosquitoes are Aedes aegypti (Aedes aegypti), Aedes Aedes mosquito ( Aedes albopictus ), Red house mosquito ( Culex pipiens pallens ), Little red house mosquito ( Culex tritaeniorhynchus ), Underground house mosquito ( Culex pipiens molestus Forskal ), Tropical house mosquito ( Culex quinquefasciatus ), Inadomia mosquito ( Culex inatomii ), Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito mosquito ( Anopheles gambiae ), small crane mosquito ( Anopheles minimus ), or Anopheles stephensi ( Anopheles stephensi ).
The method of claim 1,
The mosquito is Aedes albopictus ( Aedes albopictus ) or Red house mosquito ( Culex pipiens pallens ) The composition.
The method of claim 1,
The extract is a composition, characterized in that water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent extract thereof.
A method for controlling mosquitoes comprising the step of contacting the composition of any one of claims 1 to 5 with mosquitoes.

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