KR20220078056A - Composition and Method for Controling Mosquito Using an Extract of Lindera strychnifolia - Google Patents
Composition and Method for Controling Mosquito Using an Extract of Lindera strychnifolia Download PDFInfo
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- KR20220078056A KR20220078056A KR1020200167160A KR20200167160A KR20220078056A KR 20220078056 A KR20220078056 A KR 20220078056A KR 1020200167160 A KR1020200167160 A KR 1020200167160A KR 20200167160 A KR20200167160 A KR 20200167160A KR 20220078056 A KR20220078056 A KR 20220078056A
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- mosquito
- mosquitoes
- aedes
- extract
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Abstract
본 발명은 모기에 대해 살충 효과를 오약 추출물을 이용한 모기 방제용 조성물 및 그 방제 방법을 개시한다.The present invention discloses a composition for controlling mosquitoes and a method for controlling the same using an extract of oyak having an insecticidal effect on mosquitoes.
Description
본 발명은 오약((Lindera strychnifolia) 추출물을 이용한 모기 방제용 조성물 및 그 방제 방법에 관한 것이다.The present invention relates to a composition for controlling mosquitoes using an oyak ( ( Lindera strychnifolia ) extract and a method for controlling the same.
여름철 수면 방해, 야외 활동 등에 큰 제약을 주는 모기는 사람에게 질병을 매개하는 대표적인 해충으로, 지구상에 약 3,500종이 알려져 있고, 우리나라에서는 9속 55종이 알려져 있다. 모기는 일반적으로 교미 후 암컷 성충의 난을 성숙시키기 위해서 동물의 단백질을 얻기 위한 흡혈 활동을 하게 되며, 흡혈 부위에 병원균이나 바이러스가 포함된 타액을 분비하게 된다. 이러한 타액을 통하여 모기는 말라리아(malaria), 뇌염(encephalitis), 황열병(yellow fever), 댕기열병(dengue fever), 그리고 사상충증(filariasis) 등의 질병을 매개하는데(Science, 257:37-38, 1992), 특히 우리나라에서 자주 발생하는 일본 뇌염은 사망률이 높고 치료가 되더라도 정신박약 등 심한 후유증을 남긴다고 알려져 있다.Mosquitoes, which interfere with sleep in summer and limit outdoor activities, are representative pests that transmit diseases to humans. About 3,500 species are known on Earth, and 55 species in 9 genera are known in Korea. Mosquitoes generally engage in blood-sucking activities to obtain animal protein in order to mature female adult eggs after mating, and secrete saliva containing pathogens or viruses at the blood-sucking site. Through this saliva, mosquitoes transmit diseases such as malaria, encephalitis, yellow fever, dengue fever, and filariasis (Science, 257:37-38, 1992). ), especially Japanese encephalitis, which occurs frequently in Korea, has a high mortality rate and is known to leave severe sequelae such as mental retardation even if treated.
모기 방제를 위하여 유기인계 살충제와 피레스로이드(Pyrethroid)계 살충제가 사용되어 왔으나, 현재 가장 널리 사용되고 있는 물질은 diethyltoluamide(DEET)이다. DEET는 1946년 미군에 의해서 처음 개발되어 현재까지 모기를 비롯한 파리, 벼룩, 진드기 등의 다양한 해충들을 제어하기 위해 광범위하게 사용되어 왔다(Int. J. Pharm. 163:167-176, 1998). 그러나 피부 침투력이 높아 경피독성을 야기할 수 있고(Drug Metab. Dispos. 22:106-112, 1994) 뇌병증(encephalopathy), 급성조병형정신병(acute manic psychosis), 순환기 질환(cardiovascular) 등의 질병을 야기할 수 있다(Am. Mosq. Control Assoc. 11: 274-278, 1995). 따라서 어린이, 임산부, 저혈압 환자, 민감성 피부를 가진 사람 등에 대하여 그 사용이 제한되고 있으며, 특히 미국에서는 20% 이상 농도의 DEET를 함유하는 모기 기피제를 제조하지 못하도록 규제하고 있다.Organophosphorus insecticides and pyrethroid insecticides have been used to control mosquitoes, but the most widely used material is diethyltoluamide (DEET). DEET was first developed by the US military in 1946 and has been used extensively to control various pests such as mosquitoes, flies, fleas, and ticks until now (Int. J. Pharm. 163:167-176, 1998). However, due to its high skin penetration, it can cause transdermal toxicity (Drug Metab. Dispos. 22:106-112, 1994) and cause diseases such as encephalopathy, acute manic psychosis, and cardiovascular disease. (Am. Mosq. Control Assoc. 11: 274-278, 1995). Therefore, its use is restricted for children, pregnant women, patients with low blood pressure, and people with sensitive skin.
이러한 이유들로 최근 상대적으로 부작용이 적은 천연물을 이용한 모기 방제제를 개발하려는 노력이 이루어져 왔으며, 쇠무릎(Achyranthes bidentata) 추출물, 이슬초(Cyanotis arachnoidea) 추출물 등(한국 등록특허 제10-1778051호), 님 오일(neem oil) 등(한국 공개특허 제10-2017-0049822호), 베이(Pimenta racemosa) 추출물 등(한국 공개특허 제10-2004-0070903호), 정향유(Clove Oil) 등(한국 공개특허 제10-2012-0014536호), 황벽나무 추출물 등(한국 등록특허 제10-1470477호), 시트로넬라 정유 등(한국 공개특허 제공개특허 10-2014-0042008호)이 천연물 유래의 모기 방제제가 제안되어 있다.For these reasons, recent efforts have been made to develop mosquito control agents using natural products with relatively few side effects, such as Achyranthes bidentata extract, Cyanotis arachnoidea extract, etc. (Korean Patent No. 10-1778051), Neem oil, etc. (Korean Patent Publication No. 10-2017-0049822), Bay ( Pimenta racemosa ) extract, etc. (Korean Patent Publication No. 10-2004-0070903), Clove Oil, etc. (Korean Patent Publication) No. 10-2012-0014536), Hwangbyeok tree extract, etc. (Korean Patent No. 10-1470477), Citronella essential oil, etc. (Korean Patent Publication No. 10-2014-0042008) are natural product-derived mosquito repellents. has been proposed.
본 발명도 모기에 대해 방제 활성을 갖는 오약 추출물을 개시한다.The present invention also discloses an extract of Oyak having control activity against mosquitoes.
본 발명의 목적은 오약 추출물을 이용한 모기 방제용 조성물을 제공하는 데 있다.It is an object of the present invention to provide a composition for controlling mosquitoes using an extract of Oyak.
본 발명의 다른 목적은 오약 추출물을 이용한 모기 방제 방법을 제공하는 데 있다.Another object of the present invention is to provide a method for controlling mosquitoes using an extract of Oyak.
본 발명의 다른 목적이나 구체적인 목적은 이하에서 제시될 것이다.Other objects or specific objects of the present invention will be set forth below.
본 발명은 아래의 실시예 및 실험예에서 확인되는 바와 같이, 오약 추출물이 흰줄숲모기와 빨간집모기에 대해 살충 효과를 확인함으로써 완성된 것이다.As confirmed in the following Examples and Experimental Examples, the present invention is completed by confirming the insecticidal effect of the Oyak extract against Aedes aegypti mosquitoes and Aedes Rhesus mosquitoes.
따라서 본 발명의 모기 방제용 조성물은 오약 추출물을 유효성분으로 포함함을 특징으로 한다.Therefore, the composition for controlling mosquitoes of the present invention is characterized in that it contains an extract of Oyak as an active ingredient.
본 명세서에서, 본 명세서에서, "오약 추출물"이란 추출 대상인 오약의 잎, 줄기, 지상부, 근경, 뿌리, 지하부 또는 이들의 혼합물을 물, 탄소수 1 내지 4의 저급 알콜(메탄올, 에탄올, 부탄올 등), 메틸렌클로라이드, 에틸렌, 아세톤, 헥산, 에테르, 클로로포름, 에틸아세테이트, 부틸아세테이트, N,N-디메틸포름아미드(DMF), 디메틸설폭사이드(DMSO), 1,3-부틸렌글리콜, 프로필렌글리콜 또는 이들의 혼합 용매를 사용하여 침출하여 얻어진 추출물, 이산화탄소, 펜탄 등 초임계 추출 용매를 사용하여 얻어진 추출물 또는 그 추출물을 분획하여 얻어진 분획물을 의미하며, 추출 방법은 활성물질의 극성, 추출 정도, 보존 정도를 고려하여 냉침, 환류, 가온, 초음파 방사, 초임계 추출 등 임의의 방법을 적용할 수 있다. 분획된 추출물의 경우 추출물을 특정 용매에 현탁시킨 후 극성이 다른 용매와 혼합·정치시켜 얻은 분획물, 상기 조추출물을 실리카겔 등이 충진된 칼럼에 흡착시킨 후 소수성 용매, 친수성 용매 또는 이들의 혼합 용매를 이동상으로 하여 얻은 분획물을 포함하는 의미이다. 또한 상기 추출물의 의미에는 동결건조, 진공건조, 열풍건조, 분무건조 등의 방식으로 추출 용매가 제거된 농축된 액상의 추출물 또는 고형상의 추출물이 포함된다. 바람직하게는 추출용매로서 물, 에탄올 또는 이들의 혼합 용매를 사용하여 얻어진 추출물, 더 바람직하게는 추출용매로서 물과 에탄올의 혼합 용매를 사용하여 얻어진 추출물을 의미한다.In the present specification, as used herein, "oyak extract" refers to a leaf, stem, above-ground part, rhizome, root, underground part, or a mixture thereof of oyak, which is an extraction target, with water, a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.) , methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or these It refers to an extract obtained by leaching using a mixed solvent of In consideration of this, any method such as chilling, reflux, warming, ultrasonic radiation, and supercritical extraction may be applied. In the case of the fractionated extract, the fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and adsorbing the crude extract to a column filled with silica gel, etc. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent has been removed by methods such as freeze drying, vacuum drying, hot air drying, spray drying, and the like. Preferably, it refers to an extract obtained by using water, ethanol, or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.
또 본 명세서에서, "유효성분"이란 단독으로 목적하는 활성을 나타내거나 또는 그 자체는 활성이 없는 담체와 함께 활성을 나타낼 수 있는 성분을 의미한다.In addition, in the present specification, the term "active ingredient" means a component that can exhibit the desired activity alone or can exhibit activity together with a carrier having no activity by itself.
또 본 명세서에서, "모기 방제"는 모기 기피 또는 모기 살충을 의미한다. 여기서는 모기는 이집트숲모기(Aedes aegypti), 흰줄숲모기(Aedes albopictus) 등의 숲모기(Aedes) 속 모기, 빨간집모기(Culex pipiens pallens), 작은빨간집모기(Culex tritaeniorhynchus), 지하집모기(Culex pipiens molestus Forskal), 열대집모기(Culex quinquefasciatus), 이나도미집모기(Culex inatomii) 등의 집모기속(Culex) 속 모기, 중국얼룩날개모기(Anopheles sinensis), 감비아학질모기(Anopheles gambiae), 작은학질모기(Anopheles minimus), 아노펠레스 스테펜시(Anopheles stephensi) 등의 학질모기속(Anopheles) 모기을 의미한다.Also in this specification, "mosquito control" means mosquito repellent or mosquito repellent. Here, the mosquitoes are Aedes aegypti , Aedes albopictus , and other Aedes genus mosquitoes, Culex pipiens pallens , Culex tritaeniorhynchus, and Aedes mosquito ( Culex tritaeniorhynchus ). Culex pipiens molestus Forskal ), tropical house mosquito ( Culex quinquefasciatus ), mosquitoes of the genus Culex , such as Culex inatomii , Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito ( Anopheles gambiae ), It refers to mosquitoes of the Anopheles genus, such as Anopheles minimus and Anopheles stephensi .
본 발명의 조성물에서 그 유효성분은 모기 방제 활성 등을 나타낼 수 있는 한, 용도, 제형 등에 따라 임의의 양(유효량)으로 포함될 수 있는데, 통상적인 유효량은 조성물 전체 중량을 기준으로 할 때 0.001 중량 % 내지 20.0 중량 % 범위 내에서 결정될 것이다. 여기서 "유효량"이란 모기 방제 효과를 나타낼 수 있는, 본 발명의 조성물에 포함되는 유효성분의 양을 말한다. 이러한 유효량은 당업자의 통상의 능력 범위 내에서 실험적으로 결정될 수 있다. In the composition of the present invention, the active ingredient may be included in any amount (effective amount) depending on the use, formulation, etc. as long as it can exhibit mosquito control activity, etc., and a typical effective amount is 0.001 wt % based on the total weight of the composition to 20.0% by weight. Here, "effective amount" refers to the amount of the active ingredient included in the composition of the present invention, which can exhibit a mosquito control effect. Such an effective amount can be determined empirically within the ordinary ability of one of ordinary skill in the art.
본 발명의 조성물은 유효성분 이외에, 모기 방제 활성의 상승 보강을 위하여 당업계에서 모기 방제 활성이 있다고 알려진 임의의 화합물이나 천연물 유래 물질을 포함할 수 있다. 그러한 화합물이나 천연물 유래 물질은 예컨대 1-(이미다졸린-2-일)아미노-1,2-디페닐에탄 화합물(한국 특허출원 제2007-7029894호), 1-(1,2-디페닐-에틸)-3-(2-히드록시에틸)-티오우레아화합물(한국 특허출원 제2007-7029893호), N-(4-피리딜)메틸설폰아미드(한국 특허출원 제2007-7023622호), 2-시아노-3-(할로)알콕시-벤젠술폰아미드(한국 특허출원 제2007-7014451호), 히드라지드 화합물(한국 특허출원 제2007-7012272호), 6-(2-클로로-5-할로페닐)-트리아졸로피리미딘(한국 특허출원 제2006-7014704호), 스피로피페리딘 유도체(한국 특허출원 제2006-7011568호), 니트릴 화합물(한국 특허출원 제2006-7011448호), N-아릴히드라진 유도체(한국 특허출원 제2006-7010797호) 등을 들 수 있고, 또 앞서 예시한 바의 DEET, 쇠무릎(Achyranthes bidentata) 추출물, 이슬초(Cyanotis arachnoidea) 추출물, 님 오일(neem oil), 베이(Pimenta racemosa) 추출물, 정향유(Clove Oil), 황벽나무 추출물, 시트로넬라 정유 등을 들 수 있다. In addition to the active ingredient, the composition of the present invention may include any compound or natural product-derived material known to have mosquito control activity in the art to enhance the mosquito control activity. Such compounds or substances derived from natural products are, for example, 1-(imidazolin-2-yl)amino-1,2-diphenylethane compound (Korean Patent Application No. 2007-7029894), 1-(1,2-diphenyl- Ethyl)-3-(2-hydroxyethyl)-thiourea compound (Korean Patent Application No. 2007-7029893), N-(4-pyridyl)methylsulfonamide (Korean Patent Application No. 2007-7023622), 2 -Cyano-3-(halo)alkoxy-benzenesulfonamide (Korean Patent Application No. 2007-7014451), hydrazide compound (Korean Patent Application No. 2007-7012272), 6-(2-chloro-5-halophenyl )-triazolopyrimidine (Korean Patent Application No. 2006-7014704), spiropiperidine derivative (Korean Patent Application No. 2006-7011568), nitrile compound (Korean Patent Application No. 2006-7011448), N-arylhydrazine derivatives (Korean Patent Application No. 2006-7010797), and the like, and DEET as previously exemplified, Achyranthes bidentata extract, Cyanotis arachnoidea extract, neem oil, Pimenta racemosa ) extract, clove oil, champignon extract, and citronella essential oil.
일반적으로 식물성 오일류는 해충 기피 활성과 살충 활성을 가지는 것으로 알려져 있어(Biores. Technol. 99:2507-2515, 2008), 상기 예시한 오일류 이외에 임의의 식물 오일류도 그것이 해충 방제 활성을 가지는 한 본 발명의 조성물에 그 유효성분과 함께 포함될 수 있다. In general, vegetable oils are known to have pest repelling activity and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and any vegetable oils other than the oils exemplified above may be used as long as they have pest control activity. It may be included in the composition together with the active ingredient.
본 발명의 조성물은 유효성분 이외에 약제학적으로 허용되는 담체를 포함하여 당업계에 공지된 통상의 방법으로 그 적용 방법에 따라 임의의 제형으로 제조될 수 있다. 구체적으로는 오일, 분말, 현탁액, 훈증제, 전기 매트제, 연고제, 크림제, 로션제, 겔제, 외용액제, 파스타제, 리니멘트제, 비누, 스프레이제 등의 제형으로 제조될 수 있다. 여기서 "약제학적으로 허용되는" 의미는 유효성분의 활성을 억제하지 않으면서 인체에 적응 가능한 이상의 독성을 지니지 않는다는 의미이다. 약학적으로 허용되는 담체와 관련해서는 문헌[Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995]을 참조할 수 있으며, 위 문헌은 본 명세서의 일부로서 간주된다.The composition of the present invention can be prepared in any dosage form according to the application method by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Specifically, it may be prepared in the form of oil, powder, suspension, fumigant, electric mat, ointment, cream, lotion, gel, external solution, pasta, liniment, soap, spray, and the like. Here, "pharmaceutically acceptable" means that it does not inhibit the activity of an active ingredient and does not have more toxicity than can be adapted to the human body. Regarding pharmaceutically acceptable carriers, reference may be made to Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995, which is incorporated herein by reference.
사용될 수 있는 담체는 고체 담체, 액체 담체, 가스상 담체, 계면활성제, 분산제 등을 들 수 있는데, 고체 담체로서는 탈크, 벤토나이트, 클레이, 카올린, 규조토, 질석, 탄산 칼슘 등이 예시될 수 있고, 액체 담체로서는 메탄올, n-헥산올, 에틸렌글리콜, 아세톤, 메틸에틸케톤, 시클로헥산온, n-헥산, 케로신, 등유, 톨루엔, 크실렌, 메틸나프탈렌, 디에틸에테르, 디옥산, 테트라히드로퓨란, 에틸아세테이트, 아세토니트릴, 이소부티로니트릴, 디메틸포름아미드, 디메틸아세트아미드, 대두유, 면실유, 디메틸술폭시드, 물 등이 예시될 수 있으며, 가스상 담체로서는 LPG, 공기, 질소, 이산화탄소, 디메틸에테르 등이 예시될 수 있다.The carrier that can be used includes a solid carrier, a liquid carrier, a gaseous carrier, a surfactant, a dispersing agent, and the like. Examples of the solid carrier include talc, bentonite, clay, kaolin, diatomaceous earth, vermiculite, calcium carbonate, and the like, and a liquid carrier. As methanol, n-hexanol, ethylene glycol, acetone, methyl ethyl ketone, cyclohexanone, n-hexane, kerosine, kerosene, toluene, xylene, methyl naphthalene, diethyl ether, dioxane, tetrahydrofuran, ethyl acetate , acetonitrile, isobutyronitrile, dimethylformamide, dimethylacetamide, soybean oil, cottonseed oil, dimethyl sulfoxide, water, etc. may be exemplified, and as the gaseous carrier, LPG, air, nitrogen, carbon dioxide, dimethyl ether, etc. may be exemplified can
본 발명의 방제제 조성물은 또한 습윤제, 분산제, 계면활성제, 부동제, 증점제, 보존제, 전착제 등 하나 이상의 보조제를 포함할 수 있다.The control agent composition of the present invention may also include one or more adjuvants such as wetting agents, dispersing agents, surfactants, antifreeze agents, thickening agents, preservatives, spreading agents, and the like.
이러한 보조제는 당업계에서 공지된 것들 중에서 적절한 것을 선택하여 사용할 수 있는데, 예컨대 습윤제로서는 알킬나프탈렌 술포네이트(특히 디이소프로필나프탈렌술포네이트 또는 디이소부틸나프탈렌술포네이트)를 사용할 수 있고, 분산제로서는 비이온성 분산제(에틸렌 옥사이드/프로필렌 옥사이드 블록중합체, 알킬페놀 폴리글리콜에테르, 트리스티릴페놀 폴리글리콜 등), 음이온성 분산제(리그닌술폰산, 나프탈렌술폰산, 페놀술폰산, 디부틸나프탈렌술폰산, 알킬아릴술포네이트, 알킬 술페이트, 알킬술포네이트 등) 또는 이들의 혼합물을 사용할 수 있으며, 계면활성제로서는 알킬벤젠설포네이트, 알킬나프탈렌설포네이트, 디알킬설포석시네이트, 리그닌설포네이트, 알킬나프탈렌설포네이트포르마린축합물, 폴리옥시알킬렌알킬페닐설포네이트와 같은 설포네이트의 나트륨염 또는 칼슘염, 알킬설페이트, 폴리옥시알킬렌알킬설페이트, 폴리옥시알킬렌알킬페닐설페이트와 같은 설페이트의 나트륨염 또는 칼슘염, 나프탈렌설포석시네이트, 폴리옥시알킬렌석시네이트와 같은 석시네이트의 나트륨염 또는 칼슘염 등의 음이온성 계면활성제, 에톡실화 알킬에테르, 폴리옥시알킬렌알킬페닐폴리머, 다중 알코올 등이 사용될 수 있으며, 부동제로서는 메탄올, 에탄올, 이소프로판올, 부탄올, 글리콜, 글리세린, 디에틸렌글리콜 등을 사용할 수 있고, 증점제로서는 셀룰로즈 유도체, 폴리아크릴산 유도체, 잔탄, 개질된 점토 등을 사용할 수 있으며, 보존제로서는 디클로로펜, 이소티아졸렌, 벤질 알코올 등을 사용할 수 있으며, 전착제는 폴리비닐알코올, 폴리비닐피롤리돈, 폴리아크릴레이트, 폴리메타크릴레이트, 폴리부텐, 폴리이소부틸렌, 폴리스티렌, 폴리에틸렌아민, 폴리에틸렌아미드, 폴리에틸렌이민, 폴리에테르 등을 사용할 수 있다.Such adjuvants may be appropriately selected from those known in the art. For example, alkylnaphthalene sulfonate (especially diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) may be used as a wetting agent, and a nonionic dispersing agent may be used. Dispersing agent (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersing agent (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonate, alkyl sulfonate) paint, alkylsulfonate, etc.) or mixtures thereof, and as the surfactant, alkylbenzenesulfonate, alkylnaphthalenesulfonate, dialkylsulfosuccinate, ligninsulfonate, alkylnaphthalenesulfonate formalin condensate, polyoxy sodium or calcium salts of sulfonates such as alkylenealkylphenylsulfonate, sodium or calcium salts of sulfates such as alkylsulfate, polyoxyalkylenealkylsulfate, polyoxyalkylenealkylphenylsulfate, naphthalenesulfosuccinate; Anionic surfactants such as sodium or calcium salts of succinate such as polyoxyalkylene succinate, ethoxylated alkyl ethers, polyoxyalkylene alkylphenyl polymers, polyalcohols, etc. can be used, and the antifreeze agent is methanol, ethanol , isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used. As a thickener, cellulose derivatives, polyacrylic acid derivatives, xanthan, modified clay, etc. can be used. As a preservative, dichlorophen, isothiazolene, benzyl alcohol, etc. As the electrodeposition agent, polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc. can be used. can
다른 측면에 있어서, 본 발명은 전술한 바의 본 발명의 모기 방제용 조성물을 이용한 모기 방제 방법에 관한 것이다.In another aspect, the present invention relates to a method for controlling mosquitoes using the composition for controlling mosquitoes of the present invention as described above.
본 발명의 모기 방제 방법은 전술한 바의 본 발명의 모기 방제용 조성물을 모기와 접촉시키는 단계를 포함하여 구성된다.The mosquito control method of the present invention comprises the step of contacting the mosquito control composition of the present invention as described above with mosquitoes.
모기 방제용 조성물을 해충과 접촉시키는 단계는 본 발명의 모기 방제용 조성물을 모기가 서식하거나 분포하는 장소나 위치에 분무·살포하거나 모기가 접근하는 인체 피부 등에 도포함으로써 이루어질 수 있다.The step of contacting the composition for controlling mosquitoes with pests may be accomplished by spraying or spraying the composition for controlling mosquitoes of the present invention at a place or location where mosquitoes inhabit or distribute, or by applying the composition to human skin where mosquitoes approach.
전술한 바와 같이, 본 발명에 따르면 오약 추출물을 이용한 모기 방제용 조성물 및 그 방법을 제공할 수 있다. 본 발명의 모기 방제용 조성물은 모기 방제를 위해 모기가 서식하거나 분포하는 장소나 위치에 분무·살포되거나 모기가 접근하는 인체 피부 등에 도포되어 사용될 수 있다.As described above, according to the present invention, it is possible to provide a composition and a method for controlling mosquitoes using a oyster extract. The composition for controlling mosquitoes of the present invention may be sprayed or sprayed on a place or location where mosquitoes live or are distributed for mosquito control, or applied to human skin, etc. where mosquitoes approach.
도 1은 오약 추출물의 모기 살충 활성을 보여주는 실험 결과이다. 1 is an experimental result showing the mosquito insecticidal activity of oyak extract.
이하 본 발명을 첨부된 도면을 참조하여 바람직한 실시예를 설명한다. 그러나 본 발명의 범위가 이러한 실시예에 한정되는 것은 아니다.Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.
<< 실시예Example > 오약> Oyak 추출물의 제조 Preparation of extracts
오약 (Lindera strychnifolia) 뿌리 건조 분말 200g에 발효주정 (95%에탄올) 1L를 가하고 상온에서 5일 동안 침지하여 추출한 뒤 와트만(whatman paper) 여과지 No.2로 여과한 후 갈색의 추출용액을 얻었다. Oyak ( Lindera strychnifolia ) 1L of fermented alcohol (95% ethanol) was added to 200 g of dry root powder, immersed at room temperature for 5 days, and extracted, filtered with Whatman paper filter paper No. 2, and then a brown extract solution was obtained.
<< 실험예Experimental example > > 모기 살충 효능 실험Mosquito Insecticide Efficacy Experiment
1. 실험모기1. Experimental mosquito
실험에 사용한 흰줄숲모기(Aedes albopictus) 및 빨간집모기(Culex pipiens)는 질병관리본부에서 분양받아, 살충제에 대한 노출 없이 26±1℃, 상대습도 60±5% 조건 하에서 사육하였다. 유충은 멸균한 사료(40 메쉬 병아리 분말사료 : 효모, 1 : 1 중량비) 0.5 g이 들어 있는 플라스틱 용기(24 × 35 × 5 cm)에서 사육하였다. 성충은 10% 설탕용액을 공급하였으며 산란을 위하여 살아 있는 생쥐에 흡혈시켰다. Aedes albopictus and Culex pipiens used in the experiment were purchased from the Korea Centers for Disease Control and Prevention and bred under conditions of 26±1℃ and 60±5% relative humidity without exposure to pesticides. Larvae were reared in a plastic container (24 × 35 × 5 cm) containing 0.5 g of sterilized feed (40 mesh chick powder feed: yeast, 1:1 weight ratio). Adults were supplied with a 10% sugar solution and sucked blood to live mice for spawning.
시험에 사용한 모기는 번데기에서 우화 후 3~4일째 되는 동일 주령대의 것을 사용하였으며, 시험 모기들은 사용 전까지 혈액 대신 설탕용액을 공급하였고, 시험시작 전 12시간 정도 굶겼다. Mosquitoes used for the test were those of the same age, 3 to 4 days after emergence from the pupa, and the test mosquitoes were supplied with sugar solution instead of blood until use, and were fasted for about 12 hours before the start of the test.
2. 살충효과 검정2. Insecticide effect test
2.1. 시험 조건2.1. Exam conditions
세계보건기구(World Health Organization), Brogdon와 McAllister (1998) 및 미국질병관리센터(U.S. Centers for Disease Control)의 유리병 생물검정법(Bottle Bioassay)에 따라 살충효과를 조사하였다.The insecticidal effect was investigated according to the Bottle Bioassay of the World Health Organization, Brogdon and McAllister (1998) and the U.S. Centers for Disease Control.
2.2. 시험 방법2.2. Test Methods
용량 500 mL 유리병에 액상의 시험물질 500μL를 처리하였다. 처리 용액이 균일하게 벽면에 묻을 수 있도록 잘 흔들고 굴리듯이 하여 시험물질을 병 안쪽에 묻혔다. 후드 내에서 2시간 동안 건조시켜 용매를 완전히 휘산시키고 나서, 모기 암컷 성충 10마리씩을 넣어 주었다. 모든 실험은 3 반복으로 실시하였다. 대조구는 시험물질을 처리하지 않은 시험군(에탄올만을 처리)으로 하였다.500 μL of liquid test substance was treated in a 500 mL glass bottle. The test substance was buried inside the bottle by shaking it well and rolling it so that the treatment solution could be uniformly applied to the wall. After drying in the hood for 2 hours to completely evaporate the solvent, 10 female adult mosquitoes were added each. All experiments were performed in 3 replicates. The control group was a test group that was not treated with the test substance (treated only with ethanol).
2.3. 결과 평가2.3. result evaluation
처리구 및 무처리 대조구의 모기는 명암조건 12:12 시간, 26 ± 1°C, 상대습도 60 ± 5% 조건 하에서 방치하였으며, 처리 24시간 후에 사충율을 조사하였다. Mosquitoes in treated and untreated controls were left under light and dark conditions for 12:12 hours, 26 ± 1°C, and 60 ± 5% relative humidity, and mortality rates were investigated 24 hours after treatment.
3. 결과 - 살충 효과3. Results - insecticidal effect
시험물질의 흰줄숲모기 및 빨간집모기 암컷 성충에 대한, 3회 실험 결과의 사충율과 그 평균값을 아래의 표 1과 도 1에 나타내었다.The mortality rates and average values of the three experimental results for the adult Aedes Aedes mosquitoes and Aedes Aedes mosquitoes of the test substance and their average values are shown in Table 1 and FIG. 1 below.
Claims (6)
A composition for controlling mosquitoes comprising an extract of Oyak as an active ingredient.
상기 모기는 숲모기(Aedes) 속 모기, 집모기속(Culex) 속 모기 또는 학질모기속(Anopheles) 모기인 조성물.
According to claim 1,
The mosquitoes are Aedes genus mosquitoes, Culex genus Mosquitoes or Aedes genus Mosquitoes (Anopheles) Mosquitoes.
상기 모기는 이집트숲모기(Aedes aegypti), 흰줄숲모기(Aedes albopictus), 빨간집모기(Culex pipiens pallens), 작은빨간집모기(Culex tritaeniorhynchus), 지하집모기(Culex pipiens molestus Forskal), 열대집모기(Culex quinquefasciatus), 이나도미집모기(Culex inatomii), 중국얼룩날개모기(Anopheles sinensis), 감비아학질모기(Anopheles gambiae), 작은학질모기(Anopheles minimus), 또는 아노펠레스 스테펜시(Anopheles stephensi)인 조성물.
According to claim 1,
The mosquitoes are Aedes aegypti (Aedes aegypti), Aedes Aedes mosquito ( Aedes albopictus ), Red house mosquito ( Culex pipiens pallens ), Little red house mosquito ( Culex tritaeniorhynchus ), Underground house mosquito ( Culex pipiens molestus Forskal ), Tropical house mosquito ( Culex quinquefasciatus ), Inadomia mosquito ( Culex inatomii ), Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito mosquito ( Anopheles gambiae ), small crane mosquito ( Anopheles minimus ), or Anopheles stephensi ( Anopheles stephensi ).
상기 모기는 흰줄숲모기(Aedes albopictus) 또는 빨간집모기(Culex pipiens pallens)인 조성물.
According to claim 1,
The mosquito is Aedes albopictus ( Aedes albopictus ) or Red house mosquito ( Culex pipiens pallens ) The composition.
상기 추출물은 물, 탄소수 1 내지 4의 알콜 또는 이들의 혼합용매 추출물인 것을 특징으로 하는 조성물.
According to claim 1,
The extract is a composition, characterized in that water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent extract thereof.
A method for controlling mosquitoes comprising the step of contacting the composition of any one of claims 1 to 5 with mosquitoes.
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Citations (5)
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JP2009530421A (en) * | 2006-03-21 | 2009-08-27 | アイオワ ステート ユニバーシティー リサーチ ファウンデーション,インク. | Natural product-derived repellents derived from terpenoids and methods related thereto. |
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CN109042780A (en) * | 2018-09-29 | 2018-12-21 | 王光耀 | A kind of mosquito repellent Chinese medicine and preparation method thereof |
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KR20050109729A (en) * | 2004-05-17 | 2005-11-22 | 충북대학교 산학협력단 | The mixture composition of terpenes, having insecticidal activity |
JP2009530421A (en) * | 2006-03-21 | 2009-08-27 | アイオワ ステート ユニバーシティー リサーチ ファウンデーション,インク. | Natural product-derived repellents derived from terpenoids and methods related thereto. |
KR20100085934A (en) * | 2007-09-25 | 2010-07-29 | 키진 엔.브이. | Plant volatiles |
CN109042780A (en) * | 2018-09-29 | 2018-12-21 | 王光耀 | A kind of mosquito repellent Chinese medicine and preparation method thereof |
US20200323221A1 (en) * | 2019-04-10 | 2020-10-15 | Dien Truc Essential Oil Company Limited | Multipurpose mosquito repellant composition and method of manufacturing the same |
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