CN101049110A - Application of volatile oil of goldenrod as mosquitocide - Google Patents
Application of volatile oil of goldenrod as mosquitocide Download PDFInfo
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- CN101049110A CN101049110A CNA2006100254408A CN200610025440A CN101049110A CN 101049110 A CN101049110 A CN 101049110A CN A2006100254408 A CNA2006100254408 A CN A2006100254408A CN 200610025440 A CN200610025440 A CN 200610025440A CN 101049110 A CN101049110 A CN 101049110A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
An application of the volatile oil extracted from the plants in composite in preparing the mosquito-killing agent is disclosed.
Description
Technical field
The present invention relates to the purposes of feverfew volatile oil, relate in particular to as the application of killing the mosquito agent.
Mosquito sucks human blood, spreads disease, the serious harm human health.For a long time, people use several different methods control mosquito, and all kinds of chemical insecticides are effective and longer duration to mosquito control, play the important and pivotal role in the sick control of the mosquito matchmaker of certain areas.In people's daily life, also play irreplaceable effect at present, but the organic synthesis insecticide many problems have also been left in efficient insecticide.Facts have proved that a large amount of and use chronically particularly adds the insecticide of preventing and treating pest along with insecticide, the pesticide resistance degree of mosquito is more and more serious, and diffusion rapidly, and the environmental pollution problem of insecticide and to the influence of balance of nature also is growing on and on.They all become the outstanding problem (Lu Baolin, 1999) in the modern mosquito control.These factors impel people seek environmentally safe, be easy to degrade and have the insecticide of selecting toxicity.In recent years, the researcher drops into great amount of manpower and material resources and attempts seeking bioactivator with the instead of chemical insecticide from natural products both at home and abroad.
Plant volatile oil is a class time natural disposition metabolite in the plant corpus, and main component is monoterpene, sequiterpene and aromatic hydrocarbon derivative, how extracts from the leaf of plant, pericarp, flower, seed by steam distillation.
The monomer of plant volatile oil and its composition has received numerous scholar experts' concern to the toxicity of mosquito, and plant volatile oil mainly concentrates on the toxic action to the mosquito larvae stage the research of mosquito toxic action at present.
" The Journal of Communicable Diseases " bibliographical information, the volatile oil of Dongre etc. (1980) discovery pointed tooth Blumea balsamifera 3 kinds of Blumea balsamifera platymisciums such as (Blumea oxyodonta) can be killed northern house (Culex pipiens pipiens) larva, and wherein the volatile oil of Blumea eriantha can cause its larva dead 100% under 0.02% concentration;
" International Pest Control " bibliographical information, Namrata etc. (2000) have studied the effect of several plant volatile oil to 3 kinds of mosquitoes (anopheles stephensi Anopheles stephensi, Culex quinquefasciatus Cx.pipiens quinquefasciatus and Aedes aegypti Aedes aegypti) larva, find that the volatile oil of aztec marigold leaf demonstrates very strong activity to larva;
" Pest Management Science " bibliographical information, Traboulsi etc. (2002) have studied the toxic action of the volatile oil of several fragrant plants to Culex molestus (Culex pipiens molestus) four-age larva, found that the toxicity of Myrtus communis L is the strongest;
" Entomology " bibliographical information, Thomas etc. (2000) discover that Cannabis sativa all has certain toxic action to the larva of several mosquitoes, wherein the toxicity to Culex tritaeniorhynchus (Cx.tritaeniorhynchus) is the strongest, and product that the result shows that steam distillation the obtains product toxicity height than ethanol extraction is arranged.(1977) such as " Nature " bibliographical information Saxena find that carvacrol, citronellol, eugenol, farnesol and Mang geraniol can stop the ovum hatching of Aedes aegypti;
" Bioresource Technology " bibliographical information, Ezeonu etc. (2001) have studied plant volatile oil that two kinds of rutaceae pericarps the extract insecticidal activity to mosquito, blattaria and housefly, find effective after the insecticidal effect behind the indoor sprinkling 60min is than 30min.
Feverfew is the main weeds class of the Hai Beidiqu of Qinghai Province invasion artificially sown pastures, studies show that the synergy of composition in its volatile oil, can suppress normal Physiology and biochemistry effect in the sprouting of Elymus nutans seed and the growth of seedling process effectively, cause impaired development, even mortality, quicken the degeneration of artificially sown pastures.This plant volatile oil does not appear in the newspapers and accuses the effect that the poisoning mosquito is arranged.
Summary of the invention
The technical issues that need to address of the present invention are that open feverfew volatile oil is as the application of killing the mosquito agent.
In order better to understand essence of the present invention, test and the result of poisoning mosquito that below will be by feverfew volatile oil illustrate that it is as the application of killing the mosquito agent.
Material:
Feverfew is made volatile oil by steam distillation, meet heat and easily decompose because of seeing light, it is standby to be stored in the low-temperature dark place;
Said steam distillation all has description on general technical manual, as the method for Zhang Jijie chief editor " Natural Medicine Chemistry ";
Said feverfew (Compositae) preferably includes a kind of in the inferior chrysanthemum of Aztec marigold, thin bar sand sagebrush (Artemisia filifolia) or spire;
Culex quinquefasciatus (Culex pipiens quinquefasciatus) claim tame mosquito again, for the whole world distributes, is modal mosquito specie, is that medicine inspecting institute of China Ministry of Agriculture confirms as national standard usefulness hygienic insecticide evaluating drug effect insect;
With feverfew volatile oil and organic solvent and water, mix with following volume ratio, as testing sample:
Feverfew volatile oil 1%, organic solvent 1%, water 98%;
Said organic solvent is selected from acetone, ethanol, dimethyl sulfoxide (DMSO), glycerine or polysorbas20;
Kill the mosquito test:
Fumigating method: with reference to (2002) disclosed methods such as " Pesticide Science journal " Jiang Zhili, " the airtight fumigating system of triangular flask " inserts the female worm of 10-15 2-3d Culex quinquefasciatus in age in the 250mL triangular flask, and triangular flask is airtight with the cork that is surrounded by gauze.On cork, fix a filter paper bar (1cm * 3cm), after the 0 μ L of Dropwise 5 on the filter paper bar is for examination volatile oil, build cork rapidly with micropipettor;
Every kind of solvent repeats 3 times, and every the repetition with a batch examination worm established each solvent with equivalent simultaneously as blank, observes 6h and 24h examination worm lethality.26 ℃ ± 1 ℃ of temperature, relative moisture 65% ± 5% are carried out in all stifling test in the insectary.
The mensuration of stifling virulence and fumigation time effect:
Volatile oil to be measured is prepared into the acetone mother liquor of certain proportion (V/V), is diluted to following 5 volumetric concentration gradients with acetone by equal proportion again: 0.2%, 0.4%, 0.8%, 1.6%, 3.2%; The lethality that makes mosquito is between 5%-90%.With reference to the method (the airtight fumigating system of triangular flask) of Jiang Zhili etc. and test, in continuing to check and write down dead borer population respectively behind stifling 0.5h, 1h, 2h, 4h, 6h, the 24h.Every concentration gradient group is handled and is repeated 3 times, establishes blank simultaneously.Adopt probit analysis to calculate LC
50Value, virulence regression equation, and virulence regression equation carried out x
2Check, calculate the LC of 0.5h simultaneously
95Value (Wu Wenjun etc., 1998).LC with essential oil
95Dosage (0.5h) is handled Culex quinquefasciatus, checks and write down dead borer population every 1min, and the dead borer population of record 24h, adopts probit analysis to calculate the LT of volatile oil to Culex quinquefasciatus
50Value, virulence regression equation, and virulence regression equation carried out x
2Check (high show is quick, 1994).
Result of the test:
Said plant volatile oil has the effect of very good poisoning mosquito, can be as the agent of poisoning mosquito.Concrete outcome sees Table 1.
The composite family volatile oil solution 6h of table 1 different solvents and the lethality of 24h mosquito are relatively
| Solvent | 6h lethality (%) | 24h lethality (%) |
| Acetone ethanol DMSO glycerine polysorbas20 | 98 89 81 29 79 | 88 80 88 45 82 |
Toxicity:
Composite family volatile oil is variant in the different time periods to the stifling virulence of Culex quinquefasciatus, and blank does not all have death.When 0.5h, 1h, 2h, 4h, 6h and 24h, for the LC of examination volatile oil to Culex quinquefasciatus
50At 1h is minimum (0.203), and maximum (1.711) (table 2) during 24h.Through x
2(f=3 P=0.05) checks each virulence regression equation all to tally with the actual situation.
Table 2 composite family volatile oil is to the fumigating effect of Culex quinquefasciatus
| Time | Virulence regression equation | LC 50(％) | Correlation coefficient | 95% confidence limit | x 2 |
| 0.5h | y＝8.5788+1.7688x | 0.948 | 0.9983 | 1.327-0.677 | 3.5671 |
| 1h 2h 4h 6h 24h | y＝9.8685+1.8087x y＝9.7173+1.8428x y＝9.6326+1.9551x y＝10.14+2.4614x y＝8.9581+2.2402x | 0.203 0.276 0.427 0.816 1.711 | 0.9984 0.9984 0.9984 0.9982 0.9983 | 0.339-0.123 0.416-0.182 0.585-0.312 1.034-0.644 2.536-1.154 | 0.6264 0.6273 0.4709 0.5797 3.7865 |
Kill speed:
LC with composite family volatile oil
95Dosage, stifling Culex quinquefasciatus, the LT of handling of 2% (0.5h)
50For 8.226min (6.302-10.737,95%CI).Virulence regression equation y=2.1690+1.3434x is through x
2(f=3, P=0.05) check virulence regression equation (y=2.1690+1.3434x) all tallies with the actual situation.
The present invention for easy to use, can add organic solvent or emulsifier etc. with composite family volatile oil when concrete the use, adopt the method preparation of knowing altogether to become solvent or emulsion etc., wherein, and the weight content of composite family volatile oil preferred 0.01%～99.9%.
The above results shows, prompting composite family volatile oil has the active and certain desinsection speed of smoked kill preferably to mosquito, and said mosquito comprises Culex quinquefasciatus, anopheles stephensi, Anopheles sinensis or anopheles anthropophagus, the invention has the advantages that, the present invention has opened up the new purposes of composite family volatile oil, excavate an one new application, indicated good application prospects, less to the toxicity of composite family volatile oil, harmless, the raw material convenient sources, preparation is convenient to promote the use of easily.
Embodiment
Embodiment 1
Pulverizing product with 100 gram feverfew Aztec marigolds place 500mL distilled water, use volatile oil extractor steam distillation 5 hours, collect extract, and the centrifugal layer that anhydrates leaves standstill, and collects oil phase, is composite family volatile oil.
Embodiment 2
Pulverizing product with the thin bar sand sagebrush (Artemisia filifolia) of 100 gram feverfews place 500mL distilled water, use volatile oil extractor steam distillation 5 hours, collect extract, and the centrifugal layer that anhydrates leaves standstill, and collect oil phase, are composite family volatile oil.
Embodiment 3
The composite family volatile oil of embodiment 1 is mixed with acetone with following volume ratio:
Composite family volatile oil 1%, acetone 1% and water 98% in the container of packing into then, obtain product.
Embodiment 4
The composite family volatile oil of embodiment 1 is mixed with odorless kerosene with following part by weight, in the container of packing into then, obtain product
Composite family volatile oil 2%, odorless kerosene 98%.
Embodiment 5
With the composite family volatile oil 5% of embodiment 2, polysorbas20 2%, 93%, 50 ℃ of stirring and emulsifying of water 0.5 hour obtains emulsion.
Claims (4)
1. feverfew volatile oil is as the application of killing the mosquito agent.
2. application according to claim 1 is characterized in that, said feverfew comprises Aztec marigold, thin bar sand sagebrush (Artemisia filifolia) or the inferior chrysanthemum of spire.
3. according to the described application of claim l, it is characterized in that feverfew volatile oil is that feverfew is made volatile oil by steam distillation.
4. application according to claim 1, it is characterized in that said mosquito is Culex quinquefasciatus (Culex pipiens quinquefasciatus), anopheles stephensi (Anopheles stephensi), Anopheles sinensis (Anopheles sinensis) or anopheles anthropophagus (Anopheles lesteri).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100254408A CN101049110A (en) | 2006-04-04 | 2006-04-04 | Application of volatile oil of goldenrod as mosquitocide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100254408A CN101049110A (en) | 2006-04-04 | 2006-04-04 | Application of volatile oil of goldenrod as mosquitocide |
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| Publication Number | Publication Date |
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| CN101049110A true CN101049110A (en) | 2007-10-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2006100254408A Pending CN101049110A (en) | 2006-04-04 | 2006-04-04 | Application of volatile oil of goldenrod as mosquitocide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919702A (en) * | 2014-04-19 | 2014-07-16 | 胡素芳 | Method for preparing mosquito repelling toilet water |
| CN111771921A (en) * | 2019-12-09 | 2020-10-16 | 湖南省九喜日化有限公司 | Electric heating mosquito-repellent incense liquid and preparation method thereof |
| KR20210012240A (en) * | 2019-07-24 | 2021-02-03 | 재단법인 경기도경제과학진흥원 | Composition and Method for Controling Mosquito Using an Extract of Calendula arvensis |
| CN114831140A (en) * | 2022-05-10 | 2022-08-02 | 中国科学院新疆生态与地理研究所 | Application of Tibetan chrysanthemum indicum extract in preparation of herbicide and/or pesticide |
| CN114868772A (en) * | 2022-05-10 | 2022-08-09 | 中国科学院新疆生态与地理研究所 | Application of Tibetan chrysanthemum indicum extract as bacteriostatic agent |
-
2006
- 2006-04-04 CN CNA2006100254408A patent/CN101049110A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103919702A (en) * | 2014-04-19 | 2014-07-16 | 胡素芳 | Method for preparing mosquito repelling toilet water |
| KR20210012240A (en) * | 2019-07-24 | 2021-02-03 | 재단법인 경기도경제과학진흥원 | Composition and Method for Controling Mosquito Using an Extract of Calendula arvensis |
| KR102297598B1 (en) | 2019-07-24 | 2021-09-06 | 재단법인 경기도경제과학진흥원 | Composition and Method for Controling Mosquito Using an Extract of Calendula arvensis |
| CN111771921A (en) * | 2019-12-09 | 2020-10-16 | 湖南省九喜日化有限公司 | Electric heating mosquito-repellent incense liquid and preparation method thereof |
| CN114831140A (en) * | 2022-05-10 | 2022-08-02 | 中国科学院新疆生态与地理研究所 | Application of Tibetan chrysanthemum indicum extract in preparation of herbicide and/or pesticide |
| CN114868772A (en) * | 2022-05-10 | 2022-08-09 | 中国科学院新疆生态与地理研究所 | Application of Tibetan chrysanthemum indicum extract as bacteriostatic agent |
| CN114831140B (en) * | 2022-05-10 | 2022-10-11 | 中国科学院新疆生态与地理研究所 | Application of Tibet chrysanthemum extract in preparation of herbicide and/or pesticide |
| CN114868772B (en) * | 2022-05-10 | 2022-10-21 | 中国科学院新疆生态与地理研究所 | Application of Tibet chrysanthemum extract as bacteriostatic agent |
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