KR20240010067A - Composition and Method for Controling Mosquito Using an Extract of Tagetes patula - Google Patents

Abstract

The present invention discloses a composition for controlling mosquitoes and a method for controlling mosquitoes using a Manhydrangea extract, which has a mosquito-controlling effect.

Description

Composition and Method for Controling Mosquito Using an Extract of Tagetes patula}

The present invention relates to a composition for controlling mosquitoes using an extract of Tagetes patula and a method for controlling the same.

Mosquitoes, which disrupt sleep in the summer and greatly restrict outdoor activities, are representative pests that transmit diseases to humans. About 3,500 species are known on Earth, and 55 species in 9 genera are known in Korea. After mating, mosquitoes generally engage in blood-sucking activities to obtain animal proteins to mature the eggs of adult female insects, and secrete saliva containing pathogens or viruses into the blood-sucking area. Through this saliva, mosquitoes transmit diseases such as malaria, encephalitis, yellow fever, dengue fever, and filariasis (Science, 257:37-38, 1992) ), especially Japanese encephalitis, which occurs frequently in Korea, is known to have a high mortality rate and to leave severe aftereffects such as mental retardation even if treated.

Organophosphorus pesticides and pyrethroid pesticides have been used to control mosquitoes, but the most widely used substance currently is diethyltoluamide (DEET). DEET was first developed by the U.S. military in 1946 and has been widely used to control various pests such as mosquitoes, flies, fleas, and ticks (Int. J. Pharm. 163:167-176, 1998). However, due to its high skin penetration, it can cause transdermal toxicity (Drug Metab. Dispos. 22:106-112, 1994) and cause diseases such as encephalopathy, acute manic psychosis, and cardiovascular disease. It can be done (Am. Mosq. Control Assoc. 11: 274-278, 1995). Therefore, its use is restricted for children, pregnant women, patients with low blood pressure, people with sensitive skin, etc. In particular, in the United States, the manufacture of mosquito repellent containing DEET at a concentration of 20% or more is prohibited.

For these reasons, recent efforts have been made to develop mosquito control agents using natural products with relatively few side effects, such as Achyranthes bidentata extract, Cyanotis arachnoidea extract, etc. (Korean Patent No. 10-1778051). Neem oil, etc. (Korean Patent Publication No. 10-2017-0049822), bay ( Pimenta racemosa ) extract, etc. (Korean Patent Publication No. 10-2004-0070903), clove oil, etc. (Korean Publication Patent) No. 10-2012-0014536), Hwangbyeok tree extract, etc. (Korean Patent No. 10-1470477), citronella essential oil, etc. (Korean Published Patent No. 10-2014-0042008) are mosquito control agents derived from natural products. It is proposed.

The present invention also discloses a Manhydrangea extract that has a mosquito-controlling activity.

The purpose of the present invention is to provide a composition for controlling mosquitoes using Manhydrangea extract.

Another object of the present invention is to provide a method for controlling mosquitoes using Manhydrangea extract.

Other or specific purposes of the present invention will be presented below.

As confirmed in the examples and experimental examples below, the present invention was completed by confirming the insecticidal effect of the Manhydrangea extract on Aedes tiger mosquitoes and red house mosquitoes.

Therefore, the composition for controlling mosquitoes of the present invention is characterized by containing Manhydrangea extract as an active ingredient.

In this specification, "Manhydrangea extract" refers to the leaves, stems, aerial parts, roots, or mixtures thereof of Manhydrangea, which are the target of extraction, with water, lower alcohols with 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.), methylene chloride, ethylene, acetone, Leach using hexane, ether, chloroform, ethyl acetate, butylacetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixture of these solvents. It refers to the obtained extract, an extract obtained using a supercritical extraction solvent such as carbon dioxide or pentane, or a fraction obtained by fractionating the extract. The extraction method is cold immersion, reflux, heating, etc., considering the polarity of the active substance, degree of extraction, and degree of preservation. Any method such as ultrasonic radiation or supercritical extraction can be applied. In the case of fractionated extracts, it includes fractions obtained by suspending the extract in a specific solvent and then mixing and standing it with a solvent of different polarity, as well as fractions obtained by column chromatography. In addition, the meaning of the extract includes concentrated liquid extract or solid extract from which the extraction solvent has been removed by methods such as freeze-drying, vacuum drying, hot air drying, and spray drying. Preferably, it refers to an extract obtained using water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof as an extraction solvent, especially an extract obtained using methanol.

In addition, in this specification, “active ingredient” refers to an ingredient that can exhibit the desired activity alone or in combination with a carrier that is not active on its own.

Also, in this specification, “mosquito control” means mosquito repellent or mosquito insecticide. Here, mosquitoes include mosquitoes of the Aedes genus such as Aedes aegypti and Aedes albopictus , red house mosquitoes ( Culex pipiens pallens ), small red house mosquitoes ( Culex tritaeniorhynchus ), and underground house mosquitoes ( Mosquitoes of the Culex genus such as Culex pipiens molestus Forskal , tropical house mosquito ( Culex quinquefasciatus ), and inatomii ( Culex inatomii ), Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito ( Anopheles gambiae ), It refers to mosquitoes of the genus Anopheles, such as Anopheles minimus and Anopheles stephensi .

In the composition of the present invention, the active ingredient may be included in any amount (effective amount) depending on use, formulation, etc., as long as it can exhibit mosquito control activity, etc., and a typical effective amount is 0.001% by weight based on the total weight of the composition. It will be determined within the range of 20.0% by weight. Here, “effective amount” refers to the amount of the active ingredient included in the composition of the present invention that can exhibit a mosquito control effect. Such effective amounts can be determined experimentally within the scope of the ordinary ability of those skilled in the art.

In addition to the active ingredients, the composition of the present invention may contain any compounds or substances derived from natural products known to have mosquito control activity in the art to enhance mosquito control activity. Such compounds or substances derived from natural products include, for example, 1-(imidazolin-2-yl)amino-1,2-diphenylethane compound (Korean Patent Application No. 2007-7029894), 1-(1,2-diphenyl- Ethyl)-3-(2-hydroxyethyl)-thiourea compound (Korean Patent Application No. 2007-7029893), N-(4-pyridyl)methylsulfonamide (Korean Patent Application No. 2007-7023622), 2 -Cyano-3-(halo)alkoxy-benzenesulfonamide (Korean Patent Application No. 2007-7014451), hydrazide compound (Korean Patent Application No. 2007-7012272), 6-(2-chloro-5-halophenyl )-Triazolopyrimidine (Korean Patent Application No. 2006-7014704), spiropiperidine derivative (Korean Patent Application No. 2006-7011568), nitrile compound (Korean Patent Application No. 2006-7011448), N-arylhydrazine Derivatives (Korean Patent Application No. 2006-7010797), etc. may be mentioned, as well as DEET, Achyranthes bidentata extract, Cyanotis arachnoidea extract, neem oil, and bay ( Pimenta ) as exemplified above. racemosa ) extract, clove oil, Hwangbyeol tree extract, citronella essential oil, etc.

In general, vegetable oils are known to have pest repellent and insecticidal activity (Biores. Technol. 99:2507-2515, 2008), and in addition to the oils exemplified above, any plant oil may be used according to the present invention as long as it has pest control activity. It may be included in the composition together with the active ingredient.

The composition of the present invention can be prepared in any dosage form according to its application type by a conventional method known in the art, including solid carriers, liquid carriers, or gaseous carriers acceptable in the related art in addition to the active ingredients. Specifically, liquid, emulsion, wettable powder, water soluble powder, water dispersible granule, water soluble granule, suspension, emulsion, powder. It can be manufactured in any dosage form such as dustable powder, granule, tablet, emulsifiable gel, spray, fumigant, aerosol, or smoke agent.

Solid carriers that can be used in the composition of the present invention include natural minerals, calcined natural minerals, inorganic salts, sugars, organic substances, plant powders, etc., specifically quartz, calcite, meerschaum, dolomite, chalk, kaolinite, Phylophyllite, selicite, halosite, metahalosite, wood clay, wood clay, pottery stone, decayite, allophen, shirasu, mica, talc, bentonite, activated clay, acid clay, pumice, attapulgite. , zeolite, diatomaceous earth calcined clay, perlite, siras balloon, vermiculite, atapulgus clay, calcined diatomaceous earth, magnesium carbonate, calcium carbonate, sodium carbonate, sodium bicarbonate, ammonium sulfate, sodium sulfate, magnesium sulfate, diammonium hydrogen phosphate, ammonium dihydrogen phosphate. , potassium chloride, glucose, fructose, sucrose and lactose, starch, powdered cellulose, dextrin, urea, urea derivatives, benzoic acid, benzoic acid salts, wood flour, cork flour, corn stalks, walnut shells, tobacco stems, fly ash, white carbon (e.g. , hydrous synthetic silica, anhydrous synthetic silica, hydrous synthetic silicate, etc.), fertilizers, etc. can be used.

In addition, liquid carriers that can be used in the composition of the present invention include aromatic hydrocarbons, aliphatic hydrocarbons, alcohols, ethers, esters, amides, oils and fats, and specifically xylene, alkylbenzene, phenylxylylethane, etc. , alkylnaphthalene, machine oil, normal paraffin, isoparaffin, naphthene, ethanol, isopropanol, cyclohexanol, phenoxyethanol, benzyl alcohol, ethylene glycol, propylene glycol, diethylene glycol, hexylene glycol, polyethylene glycol, polypropylene glycol. , propyl cellosolve, butyl cellosolve, phenyl cellosolve, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, propylene glycol monobutyl ether, propylene glycol, monophenyl ether, acetophenone, Cyclohexanone, γ-butyrolactone, fatty acid methyl ester, succinic acid dialkyl ester, glutamic acid dialkyl ester, adipic acid dialkyl ester and phthalic acid dialkyl ester, N-alkylpyrrolidone, soybean oil, flaxseed oil, rapeseed oil, palm oil. , cottonseed oil, castor oil, dimethyl sulfoxide, water, etc. can be used.

Additionally, examples of gaseous carriers that can be used in the composition of the present invention include air, nitrogen, carbon dioxide, dimethyl ether, butane, propane, and halogenated hydrocarbons.

In addition to the active ingredient and the carrier ingredient, the composition of the present invention may, if necessary, additionally contain one or more auxiliaries such as surfactants, dispersants, wetting agents, antifreeze agents, thickeners, preservatives, spreaders, colorants, and stabilizers.

As the surfactant, any surfactant such as nonionic surfactant, anionic surfactant, and cationic surfactant can be used. Specifically, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenyl ether, and polyoxyethylene styryl phenyl ether. , polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, castor oil ethylene oxide adduct, acetylene glycol, acetylene alcohol, ethylene oxide adduct of acetylene glycol. , ethylene oxide adducts of acetylene alcohol, nonionic surfactants such as alkyl glycosides, alkyl sulfuric acid ester salts, alkyl benzene sulfonates, lignin sulfonates, alkyl sulfosuccinates, naphthalene sulfonates, alkyl naphthalene sulfonates, naphthalene sulfonates. Salt of formalin condensate of ponic acid, salt of formalin condensate of alkylnaphthalenesulfonic acid, polyoxyethylene alkyl ether sulfuric acid or phosphoric acid ester salt, polyoxyethylene alkyl phenyl ether sulfuric acid or phosphoric acid ester salt, polyoxyethylene styryl phenyl ether sulfuric acid or phosphoric acid. Ester salts, polycarboxylic acid salts, polystyrene sulfonates, alkylamine salts, alkylammonium salts, silicone-based surfactants, fluorine-based surfactants, etc. may be used.

Also, as dispersants, nonionic dispersants (ethylene oxide/propylene oxide block polymer, alkylphenol polyglycol ether, tristyrylphenol polyglycol, etc.), anionic dispersants (lignin sulfonic acid, naphthalenesulfonic acid, phenol sulfonic acid, dibutylnaphthalenesulfonic acid, alkylaryl sulfonate, alkyl sulfate, alkyl sulfonate, etc.) or a mixture thereof can be used, and as a wetting agent, alkylnaphthalene sulfonate (especially diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) can be used, and as an antifreeze agent Methanol, ethanol, isopropanol, butanol, glycol, glycerin, diethylene glycol, etc. can be used as thickeners, and cellulose derivatives such as carboxymethyl cellulose, polyacrylic acid derivatives, polyvinylpyrrolidone, carboxyvinyl polymer, high-purity bentonite, and xanthan. Gum, modified clay, xanthan gum, guar gum, diutane gum, starch derivatives, etc. can be used, and dichlorophene, isothiazolene, benzyl alcohol, etc. can be used as preservatives.

In addition, the electrodeposition agent can be polyvinyl alcohol, polyvinylpyrrolidone, polyacrylate, polymethacrylate, polybutene, polyisobutylene, polystyrene, polyethyleneamine, polyethyleneamide, polyethyleneimine, polyether, etc., and colorants. As a stabilizer, inorganic pigments such as iron oxide, titanium oxide, and Prussian blue, or organic pigments such as alizarin dye, azo dye, and metal phthalocyanine dye can be used. As a stabilizer, sodium benzoate, potassium sorbate, and para-hydroxybenzoic acid can be used. Preservatives such as esters, 1,2-benzothiazolin-3-one, formalin solution, freeze-thaws such as polyhydric alcohols such as ethylene glycol, diethylene glycol, propylene glycol, and glycerin, starch, alginic acid, mannose, galactose, poly Vinylpyrrolidone, ester gum, freeze inhibitor, zeolite, desiccant such as calcium oxide, magnesium oxide, antioxidant such as phenol antioxidant, amine antioxidant, sulfur antioxidant, phosphoric acid antioxidant, benzophenone ultraviolet absorber, etc. Decomposition inhibitors such as ultraviolet absorbers can be used.

For more details regarding formulation types, carriers, and auxiliaries, see "Catalogue of Pesticide Formulation Types and International Coding System" Technical Monograph, 5th Edition by CropLife International (2002), Crop Life International and in Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides, FAO Plant Production and Protection Papers - 173, prepared by the FAO/WHO Joint Meeting on Pesticide Specifications, 2004, ISBN: 9251048576], Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995], and literature [Pesticide Preparation Guide, Japanese Agrochemical Society/Application Method Research Society edition, published by Japan Plant Protection Association, 1997]. These documents, together with other documents cited herein, are considered a part of this specification.

In another aspect, the present invention relates to a method for controlling mosquitoes using the composition for controlling mosquitoes of the present invention as described above.

The mosquito control method of the present invention includes the step of contacting the mosquito control composition of the present invention as described above with mosquitoes.

The step of bringing the mosquito control composition into contact with mosquitoes can be accomplished by spraying or spraying the mosquito control composition of the present invention at a place or location where mosquitoes live or are distributed, or by applying it to human skin, etc. that mosquitoes approach.

As described above, according to the present invention, a composition and method for controlling mosquitoes using a Manhydrangea extract can be provided. The composition for mosquito control of the present invention can be used for mosquito control by spraying or spraying on a place or location where mosquitoes live or are distributed, or by applying it to human skin, etc. that mosquitoes approach.

Figure 1 shows the results showing the insecticidal effect of Manhydrangea extract on Aedes mosquitoes and red house mosquitoes.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. However, the scope of the present invention is not limited to these examples.

< Example > Preparation of Manhydrangea extract

1L of fermented alcohol (95% ethanol) was added to 200g of dried root powder of Tagetes patula and soaked at room temperature for 5 days for extraction, followed by filtration with Whatman filter paper No. 2 to obtain a yellow extraction solution.

< Experiment example > Mosquito insecticide efficacy test

1. Experimental mosquito

Aedes albopictus and Culex pipiens used in the experiment were purchased from the Korea Centers for Disease Control and Prevention and reared under conditions of 26 ± 1°C and 60 ± 5% relative humidity without exposure to pesticides. Larvae were reared in plastic containers (24 × 35 × 5 cm) containing 0.5 g of sterilized feed (40 mesh chick powder feed: yeast, 1:1 weight ratio). Adult worms were supplied with a 10% sugar solution and sucked into live mice for egg laying.

The mosquitoes used in the test were of the same age group, 3 to 4 days after emergence from pupae. The test mosquitoes were fed a sugar solution instead of blood before use, and were starved for about 12 hours before the start of the test.

2. Insecticide effect test

2.1. Exam conditions

The insecticidal effect was investigated according to the Bottle Bioassay of the World Health Organization, Brogdon and McAllister (1998), and the U.S. Centers for Disease Control.

2.2. Test Methods

After dissolving an appropriate amount of the test substance in 0.5 mL of ethanol, the test substance solution was treated in a 500 mL glass bottle for each dose. The chemical solution was applied to the inside of the bottle by shaking and rolling it well so that the treatment solution was evenly applied to the wall. After drying in the hood for about 30 minutes to volatilize the solvent, 10 female adult mosquitoes were added. All experiments were performed in 3 repetitions. The control group was a test group that was not treated with the test substance (treated only with ethanol).

2.3. Results evaluation

Mosquitoes in the treated and untreated control groups were left under conditions of 12:12 hours of light and dark, 26 ± 1°C, and 60 ± 5% relative humidity, and the insecticidal rate was examined 24 hours after treatment.

3. Result - insecticidal effect

The mortality rates by dose for female adult Aedes mosquitoes and red house mosquitoes of the test substances are shown in Table 1 and Figure 1 below. The mortality rate was found to be an average of 97.0% for Aedes mosquitoes and an average of 98.3% for red house mosquitoes.

Claims (6)

A composition for controlling mosquitoes containing Manhydrangea extract as an active ingredient.
According to paragraph 1,
A composition in which the mosquito is a mosquito of the genus Aedes , a mosquito of the genus Culex , or a mosquito of the genus Anopheles.
According to paragraph 1,
The mosquitoes include Aedes aegypti, Aedes albopictus , red house mosquito ( Culex pipiens pallens ), small red house mosquito ( Culex tritaeniorhynchus ), underground house mosquito ( Culex pipiens molestus Forskal ), and tropical house mosquito. ( Culex quinquefasciatus ), Culex inatomii , Chinese spotted wing mosquito ( Anopheles sinensis ), Gambian mosquito ( Anopheles gambiae ), Anopheles minimus , or Anopheles stephensi .
According to paragraph 1,
A composition in which the mosquito is an Aedes albopictus or a red house mosquito ( Culex pipiens pallens ).
According to paragraph 1,
A composition characterized in that the extract is water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent extract of the roots of Manhydrangea.
A method for controlling mosquitoes comprising the step of contacting a mosquito with the composition of any one of claims 1 to 5.