KR20060125081A - Manufacturing method of anticancer egg and anticancer egg - Google Patents

Abstract

A method for making functional eggs having anti-cancer property is provided to allow application of eggs as a health-aid food having anti-cancer property by feeding easily available sargassum fulvellum and gleditsia japonica var. koraensis NAKAI to chickens. The method for making functional eggs having anti-cancer property comprises the steps of: mixing sargassum fulvellum with 5-15 wt% of gleditsia japonica var. koraensis NAKAI based on the total weight of sargassum fulvellum; carrying out hydrothermal extraction of the mixture to obtain extract; diluting the extract with distilled water in a ratio of 1:40-60; feeding the diluted extract to chickens as drinking water in an amount of 150-250 ml per day; and collecting functional eggs from the chickens.

Description

Manufacturing method and anticancer egg and anticancer egg

1 is a photograph showing the cell death by BLC eggs according to the present invention

Figure 2 is a diagram showing the DNA ladder by the BLC egg according to the present invention

Figure 3 is a graph showing the change in tumor size by BLC eggs according to the present invention

Figure 4 is a photograph showing the change in tumor size caused by BLC eggs according to the present invention

5 is a graph showing the growth inhibitory effect of breast cancer cells by BLC eggs according to the present invention

The present invention relates to a method for producing anticancer functional eggs and eggs, and more specifically, to mix the mother and a juniper tree to extract the liquid or to prepare a powder, so that they can be obtained by feeding the chicken eggs.

Sargassum fulvellum is a brown algae widely distributed in the seas of Korea and Japan, and has a height of 1 to 3m and a disk shape at the bottom. The branch is triangular or triangular and twisted. The leaf is bent downward, spatula or oval, with a weak middle to the center of the leaf. Upper leaf is needle-shaped, serrate, without middle, dark yellowish brown. There are several bubbles and the shape varies according to the position, and the base of the branch is larger than the upper one. Reproductive deposits are long, columnar, with short stalks, arranged in gunshots. It grows in rocks near low ships and is used for food and feed. It is distributed in Korea and Japan.

In the prior art (Application No. 10-2003-0002053), as a result of uterine cancer cell experiment, uterine cancer cell survival rate was 0.6% and 99.4% anticancer activity was observed at 1,600 µg / ml concentration of Maban extract.

On the other hand, the juvenile tree (Gleditschia japonica var koraensis Nakai) has the leaflets inverted, the needles are small, and the stalks are inverted. Gleditsia japonica for inarmata, without thorns, a little unroasted, with a little kerchief (Gleditsia japonica var stenocarpa), with thick thorns, rounded and untwined (Gleditsia sinensis), similar to the juniper tree, but small leaves 15-35mm long, smaller, spiny, rounded in cross section similar to sculptor, and not twisted like pods of azabis. Gleditsia japonica is called juniper tree (Gleditsia Linn). Belongs to).

However, despite the fact that excellent anti-cancer function has been proved, no method has been proposed so far using the mother-child and juniper trees commonly distributed in Korea.

An object of the present invention is to obtain a functional egg excellent in anticancer properties using a mother and a juniper tree.

A feature of the present invention for achieving the above object is to obtain a cancer-fighting egg by feeding the chicken by extracting the liquid or preparing a powder by mixing the mother and a juniper tree.

The method of mixing the banban and jujube tree is to cut the banban and jujube tree evenly after each section (in the case of jujube, leaves, berries, and thorns are the same). After mixing by weight%, hot water is extracted with distilled water. Another method is to equalize evenly by section after cutting, and to dry the hatban to make 15-25% of moisture, and then pulverized by 50-150 mesh (mesh), and then to the total weight of the hatban. Mix 5-15% by weight of wood.

An embodiment of the present invention having the features as described above will be described in more detail below.

end. BLC Extract Preparation

After collecting, collecting, selecting and evaluating the origin of Sargassum fulvellum and Gleditschia japonica var koraensis Nakai, identifying the origin, cutting it down, and then evenly weighing the banban 5000g and juniper 500g. In a round flask double extracting device, 18 L of distilled water was administered, boiled at 160 ° C. for 4 hours, sterilized and filtered to prepare an extract of about 9 L (hereinafter referred to as BLC extract).

I. BLC Powder Manufacturing

After collecting, collecting, selecting, and evaluating the banban and juniper trees, identifying them, cutting them, and then evenly weighing them by area. The banban is dried to 15-25% of moisture, and then crushed into 100mesh by taking 5000g of banban and 500g of jujube. And mixed to prepare a powder (hereinafter referred to as BLC powder).

All. BLC Egg Manufacturer

The BLC extract is diluted in distilled water at a ratio of BLC extract 1: 50-60 (preferably 55) of distilled water and fed to the laying hens for 30 days for drinking. The daily amount of drinking water at an appropriate cage temperature (21 ° C.) of laying hens is 2 weeks.

Alternatively, the prepared powder is added to the general feed for laying hens by adding 10% by weight of mabanban and 1% by weight of juniper to the feed, respectively, and feeding the feed containing the powder of maban and jujube to the laying hens for 30 days. Breed by The daily feed of 1.8 kg of laying hens is about 120 g.

Looking at the characteristics of the eggs obtained from the laying hens (hereinafter referred to as BLC eggs), the egg is eggshell color of the egg is yellow and slightly fishy, whereas the anti-cancer functional egg is pale reddish and fishy It smells and tastes rather delicious.

The anticancer properties of the anticancer functional egg having the configuration as described above will be described below.

First, the concentrated powder of BLC eggs produced by laying hens, which were fed BLC extracts for 30 days without dilution, reduced the number of breast cancer cells by 30% in cell experiments of 400 µg / ml and the BLC eggs were -40 to The weight of breast cancer tumors was reduced by 71.4% in BLC-based egg powder 1000 mg / kg / day animal experiments made by vacuum, freezing and drying at -50 ° C and air pressure of 1 x 10 ^-1 mb for 30 minutes. Represents weight and is equal to or less)

In addition, in order to investigate the mechanism by which the BLC egg (BLC / HEN egg) inhibits growth in human breast cancer cells and reduces the proliferation of the cells, the BLC / HEN egg is used at a concentration of 100 µg / ml for 48 hours. After the treatment, Hoechst 33258 staining was performed to observe the morphology of the cells. As shown in FIG. 1, the proliferation of breast cancer cells was decreased in the cells treated with BLC / HEN egg as compared to the control group (Untreated). Induced nucleic acid fragmentation and condensation were observed in cells induced by apoptosis.

In addition, to confirm whether human breast cancer cells induce apoptosis when exposed to BLC / HEN egg, DNA laddering was performed. As a result, BLC / HEN egg was injected into MCF-7 cells at a concentration of 100 µg / ml for 48 hours. At the time of treatment, as shown in Fig. 2, a ladder was formed in which DNA fragmentation can be observed characteristically.

In the process of apoptosis of breast cancer cells by BLC eggs, cleavage of PARP, a death substrate related to DNA repair by increasing the expression of Bax protein that promotes cell death and reducing the expression of Bcl-2 protein that inhibits cell death. The apoptosis mechanism was thought to occur through

In addition, the BLC egg suppresses the proliferation of human breast cancer cells by increasing the expression of p53 protein, a representative cancer suppressor gene in the human body.

In addition, BLC egg concentrate powder was made of stock using EtOH, and then diluted in medium for each concentration to confirm growth inhibition effect in human breast cancer cells for 72 hours. As shown in FIG. 5, the proliferation of breast cancer cells was concentration-dependent. A tendency to decrease was observed, especially at a concentration of 400 µg / ml BLC egg concentrate powder, the inhibitory effect of human breast cancer cells was 30% compared to the control group not treated.

In addition, the BLC egg-concentrated powder was ingested with AIN-76A Diet at a dose of 4,000 ppm freely in nude mice, and the negative water was freely ingested with autoclave water, but MCF-7 cells were transplanted into mice. Human breast cancer cells, MCF-7 cells, were transplanted on the 29th day after the diet containing the BLC egg concentrate powder was fed for 28 days. After ingestion of the feed containing the same amount of BLC egg concentrate powder for 28 days after cell transplantation, which ranged from 29 to 56 days, the tumors were weighed by autopsy and compared with the control group without the BLC egg powder. % Decreased

The size change of the tumor by the BLC egg (BLC / HEN egg) is shown in Figures 3 and 4, it can be seen that the tumor is significantly reduced compared to the control.

This test was conducted by the veterinary department of Seoul National University and simultaneously with the BLC extract.

In addition, the mother board includes components such as Lg protein, Policonoid, Viotinogen, Asphaltic Acid, Alaninogen, Nitrominoid, Linanogen, Geroniolein, and Rivoflavinoid.

Among them, Lg protein inhibits prostate cancer cell growth (Koch S; 2000), and Policonoid has been proven to inhibit adult female cancer cell metastasis proliferation (Adamek HE; 1999). It has been demonstrated that uterine cancer cells have HeLa Cell main enzyme production inhibitory activity and human neural tube carcinogenesis inhibitory activity (Cus kelly G; 1999).

Biotinogen has been shown to be effective in the treatment of human pancreatic cancer (Murat A; 2000), and Avidin-biotin-peroxidase has been shown to inhibit human esophageal cancer (Raida M; 1999).

Asphaltic acid has been demonstrated to have human antitumor erythrocyte growth function (Gigoux V, Univ. Rang. France; 1999), Alaninogen has been shown to inhibit neuronal cancer cell proliferation (Wu SN; 2000), and Nitrominoid is lymphocytic leukemia Gall Cancer Cell proliferation inhibitory activity (J Tokida; 1959) and the efficacy of treatment of cancer tumor cell line has been verified (White IN; 1989).

Linanogen has been shown to inhibit cancer cell growth of human nervous system membranes (Elisabetsky E; 1999), Geroniolein has been shown to inhibit human lung and skin cancer cell growth (Yu SG, Wisconsin Univ .; 1995), and Rivoflavinoid is human oral cancer And pharyngeal cancer therapeutic efficacy (Negri E; 2000).

In addition, the above-mentioned jujube tree has nine components, such as glycidia-saponin, seryl alcohol, nonacoic acid, stigmasterol, cytosterol, phycetin, glycidsin, fustin, and triacanthin.

Among them, Gleditschia-saponine has the efficacy of anti-cancer, antibacterial, drainage, anti-inflammatory and expectorant. G. sapogenin is a non-saccharide that is produced by the decomposition of G. saponine, and is divided into tritelfenoids, steroids, and ingredients. Gleditschia-saponine Has the same potency as

Gledigenin and Gledinin have the efficacy of G. sapogenin.

Olenolic acid (caryophylline) has the same potency as tritelfenoids, echinocystic acid has the respiratory boosting effect of normal cells, and prosapogenin has the effect of G. sapogenin as the prosaccharide glycoside of sapogenin.

Pentose is composed of aldose and keto sugar, and arazinoose is a kind of aldopentose. Has the crude function of echinocystic acid.

On the other hand, in the above-described embodiment of the present invention, the anticancer experiment was performed only for breast cancer, but as described above, various components included in the components of the mother and abacus showed anticancer efficacy against various cancers in addition to breast cancer. Applicable to various cancers, including.

According to the present invention as described above it is possible to obtain an egg with anticancer function by making an extract and powder using a mother and a juniper tree easily obtained in Korea.

Claims (5)

The mother liquor, 5-15% by weight of juniper tree, and the extract obtained by hot water extraction with distilled water, extract 1: 1: distilled water 40-60 to chickens for 20-40 days a day for drinking water 150-250ml Anticancer functional egg manufacturing method characterized in that to obtain an egg by taking. The mother powder and powder composed of 5-15% by weight of juniper powder with respect to the mother powder is added to the feed for general laying hens, 5-15% by weight based on the weight of the feed to feed the chicken to obtain eggs Hamam functional egg manufacturing method characterized in that. The method according to claim 1, wherein the mother and a juniper extract extracts the mother and a juniper tree evenly divided by parts to mix 5-15% by weight of juniper to the total weight of the mother board, distilled water 3 to 1100g of the mixture Anti-cancer functional egg manufacturing method characterized in that to obtain an extract after filtering for 4 hours administration 150-200 ℃ hot water extraction at 150-200 ℃. The method of claim 2, wherein the mother board and jujube tree powder is cut evenly by the area after cutting the mother board and jujube tree, and the mother board is dried to 15-25% water content and then ground to 50-150 mesh (mesh) powder And, anti-cancer functional egg manufacturing method, characterized in that to prepare a mixed powder by mixing the juniper to 5-15% by weight relative to the total weight of the powdered mother board as described above. Anticancer functional egg, characterized in that produced by any one of claims 1 to 4.

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