KR20190093659A - 시퀀싱 반응을 위한 중합 효소 - Google Patents

시퀀싱 반응을 위한 중합 효소 Download PDF

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KR20190093659A
KR20190093659A KR1020197020661A KR20197020661A KR20190093659A KR 20190093659 A KR20190093659 A KR 20190093659A KR 1020197020661 A KR1020197020661 A KR 1020197020661A KR 20197020661 A KR20197020661 A KR 20197020661A KR 20190093659 A KR20190093659 A KR 20190093659A
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Korean (ko)
Inventor
조나단 엠. 로스버그
브라이언 리드
모함마드 와두드 부이야
만줄라 판데이
토마스 크리스티안
제레미 래키
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퀀텀-에스아이 인코포레이티드
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Priority to KR1020237012002A priority Critical patent/KR20230052995A/ko
Publication of KR20190093659A publication Critical patent/KR20190093659A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
KR1020197020661A 2016-12-19 2017-12-19 시퀀싱 반응을 위한 중합 효소 Ceased KR20190093659A (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020237012002A KR20230052995A (ko) 2016-12-19 2017-12-19 시퀀싱 반응을 위한 중합 효소

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662436410P 2016-12-19 2016-12-19
US62/436,410 2016-12-19
PCT/US2017/067427 WO2018118997A2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Related Child Applications (1)

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KR1020237012002A Division KR20230052995A (ko) 2016-12-19 2017-12-19 시퀀싱 반응을 위한 중합 효소

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KR20190093659A true KR20190093659A (ko) 2019-08-09

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KR1020197020661A Ceased KR20190093659A (ko) 2016-12-19 2017-12-19 시퀀싱 반응을 위한 중합 효소
KR1020237012002A Ceased KR20230052995A (ko) 2016-12-19 2017-12-19 시퀀싱 반응을 위한 중합 효소

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Country Link
US (2) US11312944B2 (https=)
EP (1) EP3555272A2 (https=)
JP (2) JP2020501573A (https=)
KR (2) KR20190093659A (https=)
CN (1) CN110268055A (https=)
AU (1) AU2017378990B2 (https=)
BR (1) BR112019012181A2 (https=)
CA (1) CA3047321A1 (https=)
MX (1) MX2019007248A (https=)
TW (1) TWI788318B (https=)
WO (1) WO2018118997A2 (https=)

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US20230095295A1 (en) * 2020-02-10 2023-03-30 BioSkryb Genomics, Inc. Phi29 mutants and use thereof
JP2023554368A (ja) 2020-12-15 2023-12-27 クアンタム－エスアイ インコーポレイテッド 単一分子速度論による分子バーコード分析
CN116083393B (zh) * 2023-02-14 2025-09-23 南方科技大学 一种dna装配分子机器及其应用
CN121969741A (zh) * 2023-09-25 2026-05-01 成都今是科技有限公司 用于单分子测序的核酸聚合酶及其突变株
CN119082075B (zh) * 2024-09-12 2025-06-27 湖南艾科瑞生物工程有限公司 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用

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Publication number Publication date
WO2018118997A2 (en) 2018-06-28
US20180362941A9 (en) 2018-12-20
US20180208911A1 (en) 2018-07-26
TWI788318B (zh) 2023-01-01
BR112019012181A2 (pt) 2019-11-05
JP2020501573A (ja) 2020-01-23
JP2023026651A (ja) 2023-02-24
US12545901B2 (en) 2026-02-10
CN110268055A (zh) 2019-09-20
CA3047321A1 (en) 2018-06-28
TW201835328A (zh) 2018-10-01
KR20230052995A (ko) 2023-04-20
EP3555272A2 (en) 2019-10-23
AU2017378990A1 (en) 2019-06-20
AU2017378990B2 (en) 2024-05-16
WO2018118997A3 (en) 2018-08-02
US11312944B2 (en) 2022-04-26
MX2019007248A (es) 2019-08-16
US20230058852A1 (en) 2023-02-23

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