CA3047321A1 - Polymerizing enzymes for sequencing reactions - Google Patents

Polymerizing enzymes for sequencing reactions Download PDF

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Publication number
CA3047321A1
CA3047321A1 CA3047321A CA3047321A CA3047321A1 CA 3047321 A1 CA3047321 A1 CA 3047321A1 CA 3047321 A CA3047321 A CA 3047321A CA 3047321 A CA3047321 A CA 3047321A CA 3047321 A1 CA3047321 A1 CA 3047321A1
Authority
CA
Canada
Prior art keywords
polymerizing enzyme
amino acid
recombinant
srmgqwymi
ihkerpvgyki
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3047321A
Other languages
English (en)
French (fr)
Inventor
Jonathan M. Rothberg
Brian Reed
Mohammad Wadud BHUIYA
Manjula PANDEY
Thomas CHRISTIAN
Jeremy Lackey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of CA3047321A1 publication Critical patent/CA3047321A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CA3047321A 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions Pending CA3047321A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662436410P 2016-12-19 2016-12-19
US62/436,410 2016-12-19
PCT/US2017/067427 WO2018118997A2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Publications (1)

Publication Number Publication Date
CA3047321A1 true CA3047321A1 (en) 2018-06-28

Family

ID=61148468

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3047321A Pending CA3047321A1 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Country Status (11)

Country Link
US (2) US11312944B2 (https=)
EP (1) EP3555272A2 (https=)
JP (2) JP2020501573A (https=)
KR (2) KR20190093659A (https=)
CN (1) CN110268055A (https=)
AU (1) AU2017378990B2 (https=)
BR (1) BR112019012181A2 (https=)
CA (1) CA3047321A1 (https=)
MX (1) MX2019007248A (https=)
TW (1) TWI788318B (https=)
WO (1) WO2018118997A2 (https=)

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ES2937927T3 (es) 2018-01-29 2023-04-03 St Jude Childrens Res Hospital Inc Método para la amplificación de ácidos nucleicos
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
US10865441B2 (en) * 2019-03-13 2020-12-15 Abclonal Science, Inc. Mutant Taq polymerase for faster amplification
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US11959105B2 (en) * 2019-06-28 2024-04-16 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
US11712715B2 (en) 2019-10-11 2023-08-01 Quantum-Si Incorporated Surface modification in the vapor phase
US20230095295A1 (en) * 2020-02-10 2023-03-30 BioSkryb Genomics, Inc. Phi29 mutants and use thereof
JP2023554368A (ja) 2020-12-15 2023-12-27 クアンタム－エスアイ インコーポレイテッド 単一分子速度論による分子バーコード分析
CN116083393B (zh) * 2023-02-14 2025-09-23 南方科技大学 一种dna装配分子机器及其应用
CN121969741A (zh) * 2023-09-25 2026-05-01 成都今是科技有限公司 用于单分子测序的核酸聚合酶及其突变株
CN119082075B (zh) * 2024-09-12 2025-06-27 湖南艾科瑞生物工程有限公司 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用

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Also Published As

Publication number Publication date
WO2018118997A2 (en) 2018-06-28
US20180362941A9 (en) 2018-12-20
US20180208911A1 (en) 2018-07-26
KR20190093659A (ko) 2019-08-09
TWI788318B (zh) 2023-01-01
BR112019012181A2 (pt) 2019-11-05
JP2020501573A (ja) 2020-01-23
JP2023026651A (ja) 2023-02-24
US12545901B2 (en) 2026-02-10
CN110268055A (zh) 2019-09-20
TW201835328A (zh) 2018-10-01
KR20230052995A (ko) 2023-04-20
EP3555272A2 (en) 2019-10-23
AU2017378990A1 (en) 2019-06-20
AU2017378990B2 (en) 2024-05-16
WO2018118997A3 (en) 2018-08-02
US11312944B2 (en) 2022-04-26
MX2019007248A (es) 2019-08-16
US20230058852A1 (en) 2023-02-23

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