KR20190044293A - Haemophysalis longicorni Repellent Composition Having Plant Extracts or Essential Oils - Google Patents

Abstract

The present invention relates to a Haemaphysalis longicorni avoiding composition, and more specifically, to a Haemaphysalis longicorni avoiding composition comprising an essential oil. The Haemaphysalis longicorni avoiding composition comprises (a) a Zanthoxylum piperitum extract or (b) an essential oil selected from the group consisting of Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, Camphor, Myrrh, Peppermint, and Clove bud. According to the present invention, the Zanthoxylum piperitum extract or the essential oil not only is harmless to the human body and the environment, but also has an excellent Haemaphysalis longicorni avoiding effect. Therefore, the Zanthoxylum piperitum extract or the essential oil can prevent Severe Fever with Thrombocytopenia Syndrome (SFTS) of which a preventive vaccine or an antiviral agent has not been developed till now.

Description

(Haemophysalis longicorni Repellent Composition Having Plant Extracts or Essential Oils)

The present invention relates to a composition for avoiding small mite mites, and more particularly to a composition for avoiding small mite mite including a plant extract or an essential oil.

Recently, wild ticks (aka murder ticks) are distributed throughout Korea as Haemophysalis longicorni ( Lepidoptera : Lepidoptera : Lepidoptera : Lepidoptera : Lepidoptera : Lepidoptera : Lepidoptera : Lepidoptera: Unlike animals that are parasitic, it is said that they prefer a host living in the outdoors such as mountains and fields. In particular, the small mite mite is a mediator of the severe febrile thrombocytopenic syndrome (SFTS) virus with a mortality rate of 6-10%, and a small proportion of mites belonging to the scythia virus infected with SFTS virus is 0.5%.

Symptoms of severe febrile thrombocytopenic syndrome (SFTS) include high fever at 38 to 40 ℃ for 3 to 10 days in most patients, accompanied by digestive symptoms such as vomiting, diarrhea and anorexia, platelet and leukopenia symptoms are common And it is reported that hemorrhagic symptoms such as lymph node swelling, mucous membrane or conjunctival hyperemia are seen.

Although no vaccine or antiviral agent has yet been developed, it is best to manage these mediators themselves, but the host of ticks is too diverse (eg, wild rodents, cats, birds, squirrels, deer, etc.) Due to their nature, there is a real difficulty in controlling them completely.

Therefore, there is a great need for a management strategy that can minimize the contact with small mite ticks in personal hygiene protection and outdoor activities.

Korean Patent Laid-Open Publication No. 2017-0035700 discloses a natural acaricide for mites using a compound purified from Melissa officinalis (lemon balm) essential oil and a derivative thereof, and the present applicant has disclosed in Korean Patent No. 1358643 that α-pinene α-pinene, β-pinene, α-terpinene, limonene, γ-terpinene, terpinolene, Terpinene-4-ol, α-terpineol, terpinyl acetate, isobornyl acetate and α, β-thujone. A composition containing at least one compound selected from the group consisting of a superfine supercritical extract has been disclosed, but an acarbicide or a repellent agent for a small-scale mite belonging to a more effective composition is needed.

Therefore, the present inventors have found that Eucalyptus, Citronella, Lemongrass, Lavender Bulgarian, and Lavender Bulgarian, which have not yet been known, Essential oil or Zanthoxylum piperitum extract, such as Peppermint, Clove bud, etc., have excellent avoidance effects on small mites, and the present invention has been completed .

It is an object of the present invention to provide an escaping composition that is not only harmless to the human body and environment, but also exhibits a small catching effect of a mite.

In order to accomplish the above object, the present invention provides a method for the treatment and / or prophylaxis of (a) extract of Zanthoxylum piperitum or (b) extract of Eucalyptus, Citronella, Lemongrass, Palmarosa, Characterized in that it comprises an essential oil selected from the group consisting of Lavender Bulgarian, Cinnamon, Camphor, Myrrh, Peppermint and Clove bud. A composition for avoiding small mite mites is provided.

The present invention also relates to a composition comprising (a) Zanthoxylum piperitum extract and (b) an extract of Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Characterized in that it comprises an essential oil selected from the group consisting of Bulgarian, Cinnamon, Camphor, Myrrh, Peppermint, Clove bud and Cardamon A composition for avoiding small mite mites is provided.

The present invention also provides a small soaking mite repellent made using the composition.

In the present invention, the repellent is used in at least one form of form selected from the group consisting of a liquid agent, an emulsion, a coating agent, a fuming agent, a fumigant, a granule and a solid agent.

The Zanthoxylum piperitum extract or essential oil according to the present invention is not only harmless to humans and the environment but also has an effect of avoiding small mite mites. Therefore, it has been found that Zanthoxylum piperitum extract or essential oil, which has not yet developed a preventive vaccine or antiviral agent, Thrombocytopenic syndrome (SFTS) can be prevented.

In the present invention, an attempt was made to develop a material which is harmless to humans and the environment and which has an excellent avoidance effect against a small mite mite.

In the present invention, avoidance tests for small mite mites were carried out on plant extracts and essential oils in which the avoidance effect on small mite mites was unknown. As a result, essential oils such as Eucalyptus, Citronella, Lemongrass, Lavender Bulgarian, Peppermint, Clove bud, (Zanthoxylum piperitum) extracts were found to be effective against small mites.

Accordingly, the present invention provides, in one aspect, a composition comprising (a) Zanthoxylum piperitum extract or (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Characterized in that it comprises an essential oil selected from the group consisting of Lavender Bulgarian, Cinnamon, Camphor, Myrrh, Peppermint and Clove bud. The present invention relates to a composition for avoiding a mite.

The Zanthoxylum piperitum extract and the essential oil are preferably obtained by extraction such as steam distillation or hydrodistillation or supercritical extraction.

In the present invention, the Zanthoxylum piperitum extract or essential oil is preferably contained in an amount of 0.1 to 50% by weight based on the total weight of the composition for avoiding small mites.

When the content of Zanthoxylum piperitum extract or essential oil is less than 0.1% based on the total weight, the effect of the present invention may be small and it is more than 50% by weight based on the total weight , It is difficult to exhibit a proper anti-wrinkle effect due to a relatively small amount of addition of a relatively common composition, and economical considerations may be taken into account when manufacturing a repellent.

In the present invention, it was predicted that when Zanthoxylum piperitum, which is superior in avoidance of small mite mites, is used together with other essential oils, a synergistic effect can be obtained.

In the present invention, the extract of Zanthoxylum piperitum and the extract of Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, , Camphor, Myrrh, Peppermint, Clove bud, and Cardamon were used together to avoid small swallow mites. As a result, it was confirmed that the avoidance duration was increased as compared with the case of using alone.

Accordingly, the present invention provides, in a further aspect, a composition comprising (a) Zanthoxylum piperitum extract and (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Includes essential oils selected from the group consisting of Lavender Bulgarian, Cinnamon, Camphor, Myrrh, Peppermint, Clove bud and Cardamon. To a composition for avoiding small mite mites.

The Zanthoxylum piperitum extract and the essential oil are contained in an amount of 0.1 to 50% by weight based on the total weight of the composition. If they do not fall within the above range, It is hard to get.

In another aspect, the present invention provides a small soaking mite repellent made using the composition for avoiding the small mite.

The small swallow mite repellent may be used in one or more formulations selected from the group consisting of a liquid agent, an emulsion, a coating agent, a fumigant, a fumigant, a granule and a solid agent.

The present invention relates to a method for treating microbial mites, comprising the steps of: spraying, immersion, and fumigation, which are common methods of treating acaricides.

The acaricide may be prepared and applied in various forms and may be prepared in the form of a liquid, emulsion, coating or granule by dissolving or dispersing it in one or more solvents for application and immersion treatment, optionally using a surfactant And is preferably used in the form of a fuming agent, a fumigant, an application agent, a granule and a solid agent in combination with an extender, for example, a liquid solvent, a pressurized liquefied gas and / or a solid carrier.

In order to prepare these various types of preparations, solvents and additives conventionally used in this field can be used for the essential oils according to the present invention. For example, ethanol or water, which is harmless to the human body, can be prepared into a liquid form using a dispersion, and can be prepared into a solid form using conventional excipients and the like. The microcapsules can be prepared by using poly (-caprolactone) (PCL) Can also be produced.

In addition, the concentration of the active ingredient in the acaricide is used in an effective amount to effect the acaricidal action on the mite, and the specific concentration thereof depends on the form of the preparation and the application method.

[Example]

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example 1 Evaluation of Detachability of Small Acute Mites

The inhibitory effect of essential oil and Zanthoxylum piperitum extract (supercritical fluid extract) was confirmed by the following penetration inhibition test of small mite mite ( Haemaphysalis longicornis ).

At this time, the extract of Zanthoxylum piperitum was prepared by pulverizing dried Zanthoxylum piperitum in a green-mix (DA5500) and then separating it to a particle size of 2 mm or less, ₂ supercritical fluid extraction (0.5 L)) equipment (SCFE-0500, Ilshin Autoclave). That is, the temperature of the crystallization tank was preheated to the experimental temperature (40 ° C) before injecting the sample into the crystallization tank. When the temperature of the crystallization tank is stabilized, the sample is injected into the crystallization tank by using a high-pressure pump. After the temperature has reached the steady state over time, when the CO ₂ reaches the experimental pressure of 200, 300, 400 bar through the upper line .

First, a 2.5 cm diameter circle was placed at the center of the filter paper having a diameter of 9 cm, and a thick paper was placed on the circle. 6.25% of the sample was sprayed twice from the upper part about 20 cm away from the filter paper. At this time, the injection amount per one injection was about 90 ~ 100..

After the injection, the sample was allowed to permeate the filter paper, and then the filter paper was placed in a Petri dish. Ten small mite mites were spun in the center of the sample, and small mite mites were treated at intervals of 30 minutes It was observed for 5 minutes whether or not it entered outside the circle, and the avoidance rate was evaluated according to the following formula 1, and the results are shown in Table 1. At this time, the control group was not treated and the positive control group was treated with 5% of icaridine (Bayer, Germany).

[Equation 1]

Sample (6.25%) Average Avoidance Rate (%) ± SD 10 min 30 min 60 min 90 min Control group 0 0 0 - Positive control
(Icaridin 5%) 100 91.3 ± 0.4 76.9 ± 0.9 56.2 ± 0.95 Eucalyptus 96.67 ± 0.5 73.33 + - 1.11 47.17 ± 2.23 1.30 ± 1.68 Citronella 90.33 + - 1.26 54.97 + 2.05 7.43 ± 2.75 0 Lemongrass 92.53 + - 1.15 39.33 + 1.89 1.30 ± 2.25 0 Palmarosa 76.87 ± 0.91 29.43 ± 2.77 0 0 Lavender Bulgarian 93.70 + - 1.44 40.10 ± 2.59 0 0 Cinnamon 79.63 ± 1.67 57.17 ± 1.91 6.43 + - 2.20 0 Camphor 86.1 ± 1.15 41.83 + - 2.61 1.17 + 2.02 0 Myrrh 68.57 ± 2.32 12.77 + - 2.21 0 0 Peppermint 94.00 ± 1.31 49.57 ± 2.71 9.47 ± 0.81 0 Clove bud 98.83 ± 0.29 71.93 + - 2.84 37.80 ± 1.87 3.10 ± 2.74 Cardamon 37.53 + - 3.14 0 0 0 Zanthoxyli Pericarpium Extract 100 86.57 37.73 ± 2.18 5.17 ± 1.80

As can be seen in Table 1, there were no significant differences between Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, Camphor, Myrrh, Peppermint, Clove bud oil and Zanthoxylum piperitum extracts were found to have a repellent effect on small mites such as Eucalyptus, The extracts of Clove bud and Zanthoxylum piperitum showed a repelling effect even after 90 minutes of treatment.

Example 2: Assessment of synergistic avoidance ability against small mites

In order to examine the synergistic effect of Zanthoxylum piperitum extract and other essential oils showing excellent effects in Example 1, the repellency was evaluated in the same manner as in Example 1, and the results are shown in Table 2 Respectively.

Composite ingredients (2.5% each) Average Avoidance Rate (%) ± SD 10 min 30 min 60 min 90 min Control group 0 0 0 - Positive control
(Icaridin 5%) 100 91.3 ± 0.4 76.9 ± 0.9 56.2 ± 0.95 * ZP + Eucalyptus 87.3 ± 0.96 53.1 ± 0.62 28.8 ± 1.71 0.4 ± 0.75 ZP + Citronella 81.2 ± 1.14 46.1 ± 0.97 21.3 ± 1.85 0 ZP + Lemongrass 86.0 ± 1.16 60.0 + - 1.25 32.9 ± 1.00 1.17 + 2.02 ZP + Palmarosa 87.1 ± 1.57 39.5 ± 1.25 10.4 ± 1.50 0.17 ± 0.29 ZP + Lavender Bulgarian 80.5 ± 2.21 37.5 ± 2.21 13.0 ± 0.29 0 ZP + Cinnamon 90.9 ± 1.00 61.5 ± 1.51 36.4 ± 2.36 20.8 ± 1.23 ZP + Camphor 93.9 ± 0.65 69.8 ± 1.08 35.8 ± 1.06 9.63 ± 1.21 ZP + Myrrh 88.4 ± 1.57 67.0 + 1.71 34.5 ± 2.15 0.6 ± 1.10 ZP + Peppermint 83.1 ± 1.06 45.9 ± 0.85 31.3 ± 1.25 2.9 ± 2.65 ZP + Clove bud 92.9 ± 0.70 72.8 ± 2.04 39.2 ± 0.72 16.13 + - 1.57 ZP + Cardamon 81.6 ± 0.64 53.6 ± 1.30 14.1 ± 1.73 0 ZP + Camphor 83.8 ± 1.17 45.9 ± 0.85 16.0 ± 2.11 0

* ZP: Zanthoxyli Pericarpium

As shown in Table 2, when Zanthoxylum piperitum extract and other essential oils were used together, the avoidance duration was increased and the essentials of Palmarosa, Camphor, Myrrh, It was confirmed that the synergistic effect of oil improved the ability to avoid small mites.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

(a) Zanthoxylum piperitum extract or (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, Wherein the composition comprises an essential oil selected from the group consisting of Cinnamon, Camphor, Myrrh, Peppermint and Clove bud.
[2] The composition according to claim 1, wherein the Zanthoxylum piperitum extract or essential oil is contained in an amount of 0.1 to 50% by weight based on the total weight of the composition.
(a) Zanthoxylum piperitum extract and (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, Characterized in that it comprises an essential oil selected from the group consisting of Cinnamon, Camphor, Myrrh, Peppermint, Clove bud and Cardamon. / RTI >
[4] The composition according to claim 3, wherein the Zanthoxylum piperitum extract and the essential oil are contained in an amount of 0.1 to 50% by weight based on the total weight of the composition.
A small migratory tick repellent made using the composition of claim 1 or 3.
6. The small herbivore repellent according to claim 5, wherein the repellent is used in at least one form selected from the group consisting of a liquid agent, an emulsion, a coating agent, a fumigant, a fumigant, a granule and a solid agent.