KR102065660B1 - Haemophysalis longicorni Repellent Composition Having Plant Extracts or Essential Oils - Google Patents

Abstract

The present invention relates to a small cattle tick repellent composition, and more particularly to a small cattle tick repellent composition comprising an essential oil.
The small rubbing mite repellent composition is (a) Zanthoxylum piperitum (Zanthoxylum piperitum) extract or (b) Eucalyptus (Eucalyptus), Citronella (Citronella), Lemongrass (Lemongrass), Palmarosa (Palmarosa), Lavender Essential oils selected from the group consisting of Bulgarian, Cinnamon, Campor, Myrrh, Peppermint and Clove bud.
Zanthoxylum piperitum (Zanthoxylum piperitum) extract or essential oil according to the present invention is not only harmless to the human body and the environment, but also excellent in repelling small cattle mites, severe fever that has not been developed vaccine or antiviral agent so far Thrombocytopenia syndrome (SFTS) can be prevented.

Description

Haemophysalis longicorni Repellent Composition Having Plant Extracts or Essential Oils}

The present invention relates to a small cattle mites repellent composition, and more particularly to a small cattle mites repellent composition comprising a plant extract or essential oil.

The wild mite (aka killer mite), which has become an issue recently, is distributed throughout the country as Haemophysalis longicorni , which belongs to the true mite family, and is mainly active in May-August. Unlike the parasitic in animals, they are known to prefer outdoor hosts such as mountains and fields. In particular, small-crowned tick is a mediator of severe febrile thrombocytopenia syndrome (SFTS) virus, and the mortality rate is about 6-10%, and the proportion of small-crowned tick infected with SFTS virus is 0.5%.

Symptoms of severe febrile thrombocytopenic syndrome (SFTS) are symptoms of digestive symptoms such as vomiting, diarrhea, and loss of appetite. Hemorrhagic symptoms such as lymphadenopathy, mucosal or conjunctival hyperemia have been reported.

Although vaccines and antivirals have not been developed so far, it is best to manage these carriers themselves, but the host of ticks is too diverse (eg wild rats, cats, birds, squirrels, deer, etc.) Due to their characteristics, there is a problem that it is difficult to control them completely.

Therefore, there is an urgent need for a management strategy that can minimize personal hygiene protection or contact with small ticks during outdoor activities.

Korean Patent Publication No. 2017-0035700 discloses a natural acaricide for ticks using a compound purified from Melissa essential oil (Melmar officinalis , lemon balm) essential oil and derivatives thereof, and the applicant has disclosed α-pinene ( α-pinene, β-pinene, α-terpinene, α-terpinene, limonne, γ-terpinene, terpinolene, terpinene-4- Terpinene-4-ol, α-terpineol, terpinyl acetate, isobornyl acetate and α, β-thujone Although a small rubbing mite repellent composition including a one-fold supercritical extract containing at least one compound selected from the group consisting of the disclosed, but more effective acaricides or repellents for small rubbing mites.

Therefore, the present inventors have tried to find a material having a repellent activity against small ruminant ticks, which are not known yet, as a result, Eucalyptus (Eucalyptus), Citronella (Litgrassgrass), Lemongrass (Lemongrass), Lavender Bulgarian (Lavender Bulgarian) The essential oil or Zanthoxylum piperitum extract of plant-derived essential oils such as Peppermint, Clove bud, etc. has an excellent repelling effect against small rutin mites and completes the present invention. Was done.

It is an object of the present invention to provide a repellent composition which is not only harmless to the human body and the environment, but also has excellent small repellent effect.

In order to achieve the above object, the present invention (a) Zanthoxylum piperitum (Zanthoxylum piperitum) extract or (b) Eucalyptus (Eucalyptus), Citronella (Citronella), Lemongrass (Lemongrass), Palmarosa (Palmarosa) , Essential oils selected from the group consisting of Lavender Bulgarian, Cinnamon, Campor, Myrrh, Peppermint and Clove bud Provides a composition for avoiding small cattle ticks.

The invention also relates to (a) Zanthoxylum piperitum extract and (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian And essential oils selected from the group consisting of Bulgarian, Cinnamon, Campor, Myrrh, Peppermint, Clove bud and Cardamon. Provides a composition for avoiding small cattle ticks.

The present invention also provides a small sesame mite repellent prepared using the composition.

In the present invention, the repellent is characterized in that it is used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, smokers, fumigants, granules and solids.

Zanthoxylum piperitum extract or essential oil according to the present invention is not only harmless to the human body and the environment, but also has a small fever repellent effect, so severe fever has not been developed vaccine or antiviral agent so far Thrombocytopenia syndrome (SFTS) can be prevented.

In the present invention, to develop a material having an excellent repelling effect against small cattle mites while harmless to the human body and the environment.

In the present invention, the repellent experiments for small rutin mites were carried out on plant extracts and essential oils of unknown repellent effect on small rutin mites. As a result, essential oils such as Eucalyptus, Citronella, Lemongrass, Lavender Bulgarian, Peppermint, Clove bud, and Xanthocillum piperitum (Zanthoxylum piperitum) extract was found to have a repelling effect on small cattle mites.

Accordingly, the present invention provides in one aspect: (a) Zanthoxylum piperitum extract or (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Palmarosa, Small, characterized in that it contains an essential oil selected from the group consisting of Lavender Bulgarian, Cinnamon, Campor, Myrrh, Peppermint and Clove bud It relates to a composition for avoiding small ticks.

The zanthoxylum piperitum extract and the essential oil are typically obtained by extraction such as steam distillation or hydrodistillation, supercritical extraction.

In the present invention, the Zanthoxylum piperitum (Zanthoxylum piperitum) extract or essential oil is preferably contained 0.1 to 50% by weight relative to the total weight of small rubbing mite repellent composition.

When the content ratio of the Zanthoxylum piperitum extract or essential oil is less than 0.1% of the total weight, the small cattle mite astringent effect of the present invention may be small, and more than 50% by weight of the total weight. In this case, the addition content of the relatively other general composition may be difficult to exhibit a proper acaricide effect in the blending ratio, and may also cause a problem that the economics should be taken into account in preparing the repellent.

In the present invention, the synergistic effect may be obtained by using Zanthoxylum piperitum and other essential oils, which were excellent in repelling small cattle mites when used alone.

In the present invention, Zanthoxylum piperitum extract, Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon Respiratory experiments on small cattle mites were performed using essential oils such as Camphor, Myrrh, Peppermint, Clove bud, and Cardamon. As a result, it was confirmed that the avoidance duration is increased than when used alone.

Accordingly, the present invention provides in another aspect: (a) Zanthoxylum piperitum extract and (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Palmarosa, Contains essential oils selected from the group consisting of Lavender Bulgarian, Cinnamon, Campor, Myrrh, Peppermint, Clove bud and Cardamon It relates to a small rubbing mite repellent composition, characterized in that.

The zanthoxylum piperitum extract and essential oils are characterized in that it comprises 0.1 to 50% by weight relative to the total weight of the composition, if not in the above range, as described above, has an appropriate acaricide effect Hard to get

In another aspect, the present invention provides a small sesame mite repellent prepared using the small sesame mite repellent composition.

The small tick mite repellent may be used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, smokers, fumigants, granules and solids.

Small rubbing mite repellent according to the present invention can be used a conventional treatment method of acaricides, coating treatment, immersion treatment and fumigation treatment, the application dose is also possible to apply the conventional acaricide.

The acaricide may be prepared and applied in various forms, may be prepared in the form of a liquid, emulsion, coating or granules by dissolving or dispersing in one or more solvents for coating and dipping treatment, optionally using a surfactant for fumigation treatment In combination with a bulking agent, for example a liquid solvent, pressurized liquefied gas and / or a solid carrier, and are preferably used in the form of smokers, fumigants, coatings, granules and solids.

To prepare such various types of preparations, solvents and additives commonly used in the art can be used in the essential oils according to the invention. For example, it can be prepared in liquid form using ethanol or water, which is harmless to the human body, as a dispersion, can be prepared in solid form using conventional excipients, etc., and microcapsules using poly (-caprolactone) (PCL). It can also be prepared in the form.

In addition, the concentration of the active ingredient in the acaricide is used in an amount effective to carry out acaricide mechanism with respect to the tick, the specific concentration at this time depends on the type of formulation and the method of application.

EXAMPLE

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1 Evaluation of Repellency on Small Mite Mite

The repellent effect of small oil mite ( Haemaphysalis longicornis ) was confirmed as follows, and the repellent effect of essential oil and Zanthoxylum piperitum extract (supercritical fluid extract) was confirmed.

At this time, the Zanthoxylum piperitum extract is pulverized the dried Xanthorum piperitum mixer (Green-mix; DA5500), separated to a particle size of less than 2mm and then a supercritical fluid extractor for research (CO ₂ supercritical fluid extraction (0.5L) equipment (SCFE-0500, Ilshin Autoclave) was extracted. That is, the temperature of the crystallization tank was preheated to the experiment temperature (40 ° C) before the sample was injected into the crystallization tank. When the temperature of the crystallization tank is stabilized, the sample is injected into the crystallization tank by using a high pressure pump. When the temperature reaches a steady state over time, when CO ₂ is reached at 200, 300, 400 bar through the upper line, respectively. A certain amount was injected until.

First, a 2.5cm diameter circle was placed in the center of a 9cm diameter filter paper and a thick paper was placed on the circle. 6.25% of the sample was sprayed twice at the top about 20 cm away from the filter paper. At this time, the injection amount was about 90 ~ 100㎛.

After the injection, the sample was allowed to penetrate the filter paper for 10 minutes, and then the filter paper was placed in a petri dish, and then 10 small blue ticks were sprayed at the center of the sample-free circle. It was observed for 5 minutes whether it penetrated out of a circle, the evasion rate was evaluated according to following formula (1), and the result is shown in Table 1. At this time, the control group did not process anything, and the positive control group was treated with 5% of Icaridine (Bayer, Germany), and the experimental result is the average value repeated three times.

[Equation 1]

Sample (6.25%) Average Evasion Rate (%) ± SD 10 min 30 min 60 min 90 min Control 0 0 0 - Positive control
(Icaridin 5%) 100 91.3 ± 0.4 76.9 ± 0.9 56.2 ± 0.95 Eucalyptus 96.67 ± 0.5 73.33 ± 1.11 47.17 ± 2.23 1.30 ± 1.68 Citronella 90.33 ± 1.26 54.97 ± 2.05 7.43 ± 2.75 0 Lemongrass 92.53 ± 1.15 39.33 ± 1.89 1.30 ± 2.25 0 Palmarosa 76.87 ± 0.91 29.43 ± 2.77 0 0 Lavender bulgaria 93.70 ± 1.44 40.10 ± 2.59 0 0 Cinnamon 79.63 ± 1.67 57.17 ± 1.91 6.43 ± 2.20 0 Camphor 86.1 ± 1.15 41.83 ± 2.61 1.17 ± 2.02 0 Myrrh 68.57 ± 2.32 12.77 ± 2.21 0 0 Peppermint 94.00 ± 1.31 49.57 ± 2.71 9.47 ± 0.81 0 Clove bud 98.83 ± 0.29 71.93 ± 2.84 37.80 ± 1.87 3.10 ± 2.74 Cardamon 37.53 ± 3.14 0 0 0 Zanthoxyli Pericarpium Extract 100 86.57 37.73 ± 2.18 5.17 ± 1.80

As shown in Table 1, Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, Camper, Mir ( Myrrh, Peppermint, Clove bud oil, and Zanthoxylum piperitum extracts have been found to have a repelling effect on small ticks, especially Eucalyptus, Clove. Bud (Clove bud) and Zanthoxylum piperitum (Zanthoxylum piperitum) extract was found to have a repelling effect up to 90 minutes after treatment.

Example 2 Evaluation of Synergy Avoidance on Small Mite Mite

In order to examine the synergistic effect of Zanthoxylum piperitum extract and other essential oils showing excellent effects in Example 1, the repellency was evaluated in the same manner as in Example 1, and the results are shown in Table 2 Shown in

Complex ingredients (2.5% each) Average Evasion Rate (%) ± SD 10 min 30 min 60 min 90 min Control 0 0 0 - Positive control
(Icaridin 5%) 100 91.3 ± 0.4 76.9 ± 0.9 56.2 ± 0.95 * ZP + Eucalyptus 87.3 ± 0.96 53.1 ± 0.62 28.8 ± 1.71 0.4 ± 0.75 ZP + Citronella 81.2 ± 1.14 46.1 ± 0.97 21.3 ± 1.85 0 ZP + Lemongrass 86.0 ± 1.16 60.0 ± 1.25 32.9 ± 1.00 1.17 ± 2.02 ZP + Palmarosa 87.1 ± 1.57 39.5 ± 1.25 10.4 ± 1.50 0.17 ± 0.29 ZP + Lavender Bulgarian 80.5 ± 2.21 37.5 ± 2.21 13.0 ± 0.29 0 ZP + Cinnamon 90.9 ± 1.00 61.5 ± 1.51 36.4 ± 2.36 20.8 ± 1.23 ZP + Camphor 93.9 ± 0.65 69.8 ± 1.08 35.8 ± 1.06 9.63 ± 1.21 ZP + Myrrh 88.4 ± 1.57 67.0 ± 1.71 34.5 ± 2.15 0.6 ± 1.10 ZP + Peppermint 83.1 ± 1.06 45.9 ± 0.85 31.3 ± 1.25 2.9 ± 2.65 ZP + Clove bud 92.9 ± 0.70 72.8 ± 2.04 39.2 ± 0.72 16.13 ± 1.57 ZP + Cardamon 81.6 ± 0.64 53.6 ± 1.30 14.1 ± 1.73 0 ZP + Camphor 83.8 ± 1.17 45.9 ± 0.85 16.0 ± 2.11 0

* ZP: Zanthoxyli Pericarpium

As shown in Table 2, when the Zanthoxylum piperitum extract and other essential oils were used together, the avoidance duration increased, and essentials such as Palmarosa, Camphor, Myrrh, etc. The oil was found to improve the repellent ability for small rubbing mite due to the synergistic effect than using alone.

As described above in detail specific parts of the present invention, it will be apparent to those skilled in the art that these specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. will be. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

(a) Zanthoxylum piperitum extract or (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon (Cinnamon), Camper (Camphor), Peppermint (Peppermint) and Clove bud (Clove bud) comprising a essential oil selected from the group consisting of small rubbing mite repellent composition.
According to claim 1, wherein the Zanthoxylum piperitum (Zanthoxylum piperitum) extract or essential oil composition for avoiding small mite mites, characterized in that containing 0.1 to 50% by weight relative to the total weight of the composition.
(a) Zanthoxylum piperitum extract and (b) Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon (Cinnamon), Camper (Camphor), Peppermint (Peppermint), Clove bud (Clove bud) and Cardamon (Cardamon) essential oils selected from the group consisting of small repellent mite repellent composition characterized in that it comprises.
According to claim 3, The xanthoxylum piperitum (Zanthoxylum piperitum) extract and essential oil composition for avoiding small mite mites, characterized in that it comprises 0.1 to 50% by weight relative to the total weight of the composition.
Small tick mite repellent comprising the composition of claim 1 or 3.
The small repellent mite repellent of claim 5, wherein the repellent is used in the form of a formulation selected from the group consisting of liquids, emulsions, coatings, fumigants, fumigants, granules and solid agents.