KR102015366B1 - Haemophysalis longicorni Repellent Composition - Google Patents

Abstract

The present invention relates to a small cattle mites repellent composition, and more particularly, to a small cattle mites repellent composition comprising a plant-derived compound.
The small rubbing mite repellent composition is (-)-limonene oxide (Limonene oxide), (+)-limonene oxide (Limonene oxide), (±)-citronellal (Citronellal), 3- ethylene (Carene), cinnamic aldehyde 1 or more compounds selected from the group consisting of (cinnamaldehyde), citral, eucalyptol, menthol, menthone, thymol and β-caryophyllene do.
The compound according to the present invention is not only harmless to the human body and the environment, but also has a small small mite repelling effect, and thus can prevent severe febrile thrombocytopenic syndrome (SFTS), which has not yet been developed as a vaccine or antiviral agent.

Description

Haemophysalis longicorni Repellent Composition

The present invention relates to a small cattle mites repellent composition, and more particularly, to a small cattle mites repellent composition comprising a plant-derived compound.

The wild mite (aka killer mite), which has become an issue recently, is distributed throughout the country as Haemophysalis longicorni , which belongs to the true mite family, and is mainly active in May-August. Unlike the parasitic in animals, they are known to prefer outdoor hosts such as mountains and fields. In particular, small-crowned ticks are mediators of severe febrile thrombocytopenic syndrome (SFTS) virus, and the mortality rate is around 6-10%, and the proportion of small-crowned ticks infected with SFTS virus is 0.5%.

Symptoms of severe febrile thrombocytopenic syndrome (SFTS) are associated with digestive symptoms such as vomiting, diarrhea, and loss of appetite. Hemorrhagic symptoms such as lymphadenopathy, mucosal or conjunctival hyperemia have been reported.

Although vaccines and antivirals have not been developed so far, it is best to manage these carriers themselves, but the host of ticks is too diverse (eg wild rats, cats, birds, squirrels, deer, etc.) Due to its characteristics, there is a problem that it is practically difficult to control them completely.

Therefore, there is an urgent need for a management strategy that can minimize personal hygiene protection or contact with small ticks during outdoor activities.

Korean Patent Publication No. 2017-0035700 discloses a natural acaricide for ticks using a compound purified from Melissa essential oil (Melmar officinalis , lemon balm) essential oil and derivatives thereof, and the applicant has disclosed α-pinene ( α-pinene, β-pinene, α-terpinene, α-terpinene, limonne, γ-terpinene, terpinolene, terpinene-4- Terpinene-4-ol, α-terpineol, terpinyl acetate, isobornyl acetate and α, β-thujone Although the composition for repelling small cattle mites containing one or more supercritical extracts containing one or more compounds selected from the group consisting of the above, there is a need for acaricides or repellents for small cattle mites excellent effect.

Accordingly, the present inventors have tried to find a material having a repellent activity against small rubbing mite, which is not yet known, (-)-limonene oxide (Limonene oxide), (+)-limonene oxide (Limonene oxide), (±) Citronellal, 3-Carene, Cinnanamaldehyde, Citral, Eucalyptol, Menthol, Menthone, Thimol, β- It was confirmed that essential oil-derived compounds such as Caryophyllene have an excellent repelling effect against small cattle mites, and the present invention was completed.

It is an object of the present invention to provide a repellent composition which is not only harmless to the human body and the environment, but also has excellent small repellent effect.

In order to achieve the above object, the present invention is (-)-limonene oxide (Limonene oxide), (+)-limonene oxide (Limonene oxide), (±)-citronellal (Citronellal), 3-Carene (Carene), Compounds selected from the group consisting of cinnamaldehyde, citral, eucalyptol, menthol, menthone, thymol and β-caryophyllene It provides a small rubbing mite repellent composition comprising the above.

In the present invention, the compound is characterized in that it comprises 0.1 to 50% by weight relative to the total weight of the composition.

The present invention also provides a small tick mite repellent prepared using the composition.

In the present invention, the repellent is characterized in that it is used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, smokers, fumigants, granules and solids.

The compound according to the present invention is not only harmless to the human body and the environment, but also has a small small mite repelling effect, and thus can prevent severe febrile thrombocytopenic syndrome (SFTS), which has not yet been developed as a vaccine or antiviral agent.

In the present invention, to develop a material having an excellent repelling effect against small cattle mites while harmless to the human body and the environment.

In the present invention, the repellent experiment was performed on essential oil and plant extracts of unknown oils and plant extracts for which the repellent effect is not known. As a result, essential oils such as Eucalyptus, Citronella, Lemongrass, Lavender Bulgarian, Peppermint, Clove bud, and Xanthocillum piperitum (Zanthoxylum piperitum) extract was found to have a repelling effect on small cattle mites.

In the present invention, in order to identify a compound having a higher repellent activity, the evacuation evaluation of small sesame mites was performed on a single component constituting the essential oil having a repelling effect on small sesame mites and analogs thereof. As a result, (-)-limonene oxide (Limonene oxide), (+)-limonene oxide (Limonene oxide), (±)-citronellal (Citronellal), 3-carene (Carene), cinnanamaldehyde (cinnamaldehyde), sheet Ral (Citral), Eucalyptol (Eucalyptol), Menthol (Menthol), Menthone (Menthone), thymol (Thymol), β- Caryophyllene (Caryophyllene) has been found to have a repelling effect on small cattle mites.

Accordingly, the present invention is, in one aspect, (-)-limonene oxide (Limonene oxide), (+)-limonene oxide (Limonene oxide), (±)-citronellal (Citronellal), 3-Carene (Carene), Cinnam One or more compounds selected from the group consisting of aldehyde (cinnamaldehyde), citral, eucalyptol, menthol, menthone, thymol and β-caryophyllene It relates to a small rubbing mite repellent composition comprising a.

In the present invention, the compound is preferably contained 0.1 to 50% by weight relative to the total weight of small rubbing mite repellent composition.

When the content of the compound is less than 0.1% of the total weight, the small agar mite astringent effect that is desired in the present invention may be small, and when it exceeds 50% by weight of the total weight, the content of relatively other common compositions is increased. It may be difficult to show a suitable fertilizing effect in proportion to the blending ratio, and may also cause a problem in that economics should be taken into account in preparing the repellent.

In another aspect, the present invention provides a small sesame mite repellent prepared using a small sesame mite repellent composition comprising the compound.

The small tick mite repellent may be used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, smokers, fumigants, granules and solids.

Small rubbing mite repellent according to the present invention can be used a conventional treatment method of acaricides, coating treatment, immersion treatment and fumigation treatment, the application dose is also possible to apply the conventional acaricide.

The acaricide may be prepared and applied in various forms, may be prepared in the form of a liquid, emulsion, coating or granules by dissolving or dispersing in one or more solvents for coating and dipping treatment, optionally using a surfactant for fumigation treatment In combination with a bulking agent, for example a liquid solvent, pressurized liquefied gas and / or a solid carrier, and are preferably used in the form of smokers, fumigants, coatings, granules and solids.

To prepare such various forms of preparations, solvents and additives conventionally used in the art can be used for the compounds according to the invention. For example, it can be prepared in liquid form using ethanol or water, which is harmless to the human body, as a dispersion, can be prepared in solid form using conventional excipients, etc., and microcapsules using poly (-caprolactone) (PCL). It can also be prepared in the form.

In addition, the concentration of the active ingredient in the acaricide is used in an amount effective to carry out acaricide mechanism with respect to the tick, the specific concentration at this time depends on the type of formulation and the method of application.

EXAMPLE

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1 Evaluation of Repellency on Small Mite Mite

The repellent effect of small oil mite ( Haemaphysalis longicornis ) was confirmed as follows, and the repellent effect of essential oil and Zanthoxylum piperitum extract (supercritical fluid extract) was confirmed.

At this time, the Zanthoxylum piperitum extract is pulverized the dried Xanthorum piperitum mixer (Green-mix; DA5500), separated to a particle size of less than 2mm and then a supercritical fluid extractor for research (CO ₂ supercritical fluid extraction (0.5L) equipment (SCFE-0500, Ilshin Autoclave) was extracted. That is, the temperature of the crystallization tank was preheated to the experiment temperature (40 ° C) before the sample was injected into the crystallization tank. When the temperature of the crystallization tank is stabilized, the sample is injected into the crystallization tank by using a high pressure pump, and when the temperature reaches a steady state over time, when CO ₂ is reached at 200, 300, 400 bar through the upper line, respectively. A certain amount was injected until.

First, a 2.5cm diameter circle was placed in the center of a 9cm diameter filter paper and a thick paper was placed on the circle. 6.25% of the sample was sprayed twice at the top about 20 cm away from the filter paper. At this time, the injection amount was about 90 ~ 100㎛.

After 10 minutes of injection, allow the sample to penetrate the filter paper, insert the filter paper into the petri dish, and then spin 10 small blue ticks in the center of the sample-free circle. It was observed for 5 minutes whether it penetrated out of a circle, the evasion rate was evaluated according to following formula (1), and the result is shown in Table 1. At this time, the control group did not process anything, and the positive control group was treated with 5% of Icaridine (Bayer, Germany), and the experimental result is the average value repeated three times.

[Equation 1]

Sample (6.25%) Average Evasion Rate (%) ± SD 10 min 30 min 60 min 90 min Control 0 0 0 - Positive control
(Icaridin 5%) 100 91.3 ± 0.4 76.9 ± 0.9 56.2 ± 0.95 Eucalyptus 96.67 ± 0.5 73.33 ± 1.11 47.17 ± 2.23 1.30 ± 1.68 Citronella 90.33 ± 1.26 54.97 ± 2.05 7.43 ± 2.75 0 Lemongrass 92.53 ± 1.15 39.33 ± 1.89 1.30 ± 2.25 0 Palmarosa 76.87 ± 0.91 29.43 ± 2.77 0 0 Lavender bulgaria 93.70 ± 1.44 40.10 ± 2.59 0 0 Cinnamon 79.63 ± 1.67 57.17 ± 1.91 6.43 ± 2.20 0 Camphor 86.1 ± 1.15 41.83 ± 2.61 1.17 ± 2.02 0 Myrrh 68.57 ± 2.32 12.77 ± 2.21 0 0 Peppermint 94.00 ± 1.31 49.57 ± 2.71 9.47 ± 0.81 0 Clove bud 98.83 ± 0.29 71.93 ± 2.84 37.80 ± 1.87 3.10 ± 2.74 Cardamon 37.53 ± 3.14 0 0 0 Zanthoxyli Pericarpium Extract 100 86.57 37.73 ± 2.18 5.17 ± 1.80

As shown in Table 1, Eucalyptus, Citronella, Lemongrass, Palmarosa, Lavender Bulgarian, Cinnamon, Camper, Mir ( Myrrh, Peppermint, Clove bud oil and Zanthoxylum piperitum extracts have been found to have a repelling effect on small mite mites, especially Eucalyptus, Clove Bud (Clove bud) and Zanthoxylum piperitum (Zanthoxylum piperitum) extract was found to have a repelling effect up to 90 minutes after treatment.

Example 2: GC-MS Analysis

In order to identify the compounds constituting the essential oil and Zanthoxylum piperitum extract which have been found to have a repellent effect against small mite mite in Example 1, 0.01 g of the sample was dissolved in 1 ml of hexane and centrifuged. The supernatant was then obtained, filtered through a micro filter (0.45 μm) and analyzed by GC / MS. The analysis was performed using the GC / MS equipment (240-MS, Varian) under the following conditions. VF-5ms (30mm × 0.25mm × 0.25mm) for column, He (1mL / min) for carrier gas, Injection temperature is 250 ℃, Oven temperature is 50 ~ 250 ℃ / 3 ℃, Injection volume is 1µl, injection The component was analyzed under the split ratio of 20: 1, the mass range was 28 ~ 550 in the MDS (mass selective detector), and the acquisition mode was analyzed under the scan mode. The main components are shown in Table 2.

No. Samples Major peak One Eucalyptus dl-Limonene, α-Terpinyl acetate, α-Pinene 2 Citronella Geraniol, Camphene, dl-Limonene 3 Lemongrass E-citral, Nerol, Z-citral 4 Peppermint L-Mentho, 1-Menthone, Menthofuran 5 Lavender bulgaria β-Ocimene-x, Linalyl acetate, 1,3,6-Octatriene 6 Peppermint L-Menthol, l-Menthone, Iso-Methyl acetate 7 Clove bud Eugenol, trans-Caryophellene, Iso-Eugenol 8 Zanthoxyli Pericarpium Deca- (2E, 4E) -dienal, Hept- (2E) -enal, Hexanoic acid

Example 3: Evaluation of repellency of small compound mite mites of a single compound

In order to identify compounds exhibiting a repelling effect on small rutin mites of the single compound identified in Example 2 and analogue compounds added thereto, the repellency of small rutin mites was evaluated in the same manner as in Example 1, The results are shown in Table 3.

Compound (6.25%) Average Evasion Rate (%) ± SD 10 min 30 min 60 min 90 min 120 min Control 0 0 0 - - Positive control
(Icaridin 5%) 100 91.3 ± 0.4 76.9 ± 0.9 56.2 ± 0.95 41.2 ± 1.25 (-)-Limonene oxide 90.73 ± 1.39 78.57 ± 1.8 38.10 ± 2.03 0.65 ± 1.13 0 (-)-Verbenone 4.13 ± 3.72 0 0 0 0 (-)-β-Pinene 96.77 ± 2.87 85.94 ± 1.21 51.63 ± 1.75 12.9 ± 2.36 0 (+)-Limonene oxide 94.10 ± 0.85 77.10 ± 0.85 44.63 ± 2.15 14.33 ± 2.80 0 (+)-Pulegone 75.63 ± 1.99 42.07 ± 1.91 6.87 ± 2.17 0 0 (+)-α-Pinene 100 85.53 ± 1.10 57.53 ± 1.72 31.27 ± 1.57 0 (+)-β-Pinene 100 86.10 ± 1.08 61.93 ± 1.31 32.28 ± 1.10 1.9 ± 2.87 (±) -Citronellal 100 98.3 ± 2.94 78.2 ± 1.83 37.47 ± 2.47 3.99 ± 3.55 (1R)-(+)-α-Pinene 87.50 ± 1.00 76.47 ± 1.25 52.27 ± 1.08 15.83 ± 2.25 0 (R)-(+)-Limonene 82.30 ± 2.34 49.47 ± 2.71 17.97 ± 4.38 6.30 ± 2.45 0 (S)-(-)-Limonene 83.20 ± 1.83 40.47 ± 1.27 6.30 ± 2.45 0 0 1-Undecyne 0 0 0 0 0 3-Carene 85.20 ± 2.00 66.53 ± 1.65 32.17 ± 2.76 1.73 ± 3.00 0 Camphor 48.40 ± 4.00 19.07 ± 1.25 0 0 0 cinnamaldehyde 100 100 82.97 ± 1.27 51.53 ± 1.91 34.77 ± 2.14 Citral 90.17 ± 1.42 50.40 ± 1.23 30.80 ± 1.15 3.07 ± 3.15 0 Eucalyptol 97.37 ± 2.55 86.43 ± 1.08 65.87 ± 1.97 34.97 ± 1.96 2.70 ± 2.61 Geraniol 1.73 ± 3.00 0 0 0 0 Menthol 85.53 ± 1.53 63.40 ± 0.35 31.90 ± 2.18 0 0 Menthone 81.83 ± 1.43 50.10 ± 1.71 15.67 ± 2.63 0.17 ± 0.29 0 Sabinene hydrate 56.13 ± 2.54 14.17 ± 2.11 0 0 0 Terpineol 97.90 ± 1.85 80.83 ± 1.88 40.10 ± 0.62 5.07 ± 1.80 0 Thymol 98.93 ± 0.95 87.13 ± 1.45 36.57 ± 2.69 18.50 ± 1.70 0.17 ± 0.29 Valencene 0 0 0 0 0 α-Pinene oxide 0.60 ± 1.04 0 0 0 0 β-Caryophyllene 80.20 ± 1.85 47.17 ± 1.45 9.00 ± 1.23 0 0

As shown in Table 3, (-)-limonene oxide (Limonene oxide), (+)-limonene oxide (Limonene oxide), (±)-citronellal (Citronellal), 3-carene (Carene), cinnamic aldehyde ( cinnamaldehyde, citral, eucalyptol, menthol, menthone, thymol, β-caryophyllene, etc. In particular, α-pinene (α-pinene), α-terpinene (α-terpinene), terpinene-4-ol (terpinene-4-ol) and α-terpineol (α-terpineol) It was confirmed that the repellent effect was excellent up to 120 minutes after the treatment.

As described above in detail specific parts of the present invention, it will be apparent to those skilled in the art that these specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. will be. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (4)

3-Carene (β) and β- Caryophyllene (Caryophyllene) A small cough mite repellent composition comprising at least one compound selected from the group consisting of.
The method of claim 1, wherein the compound is characterized in that less than 0.1% to 50% by weight relative to the total weight of the composition.
Small small tick mite repellent prepared using the composition of claim 1 or 2.
4. The repellent of claim 3, wherein the repellent is used in the form of one or more formulations selected from the group consisting of liquids, emulsions, coatings, fumigants, fumigants, granules and solid agents.