KR20190012743A - Fish cake contained undenatured collagen type II and manufacturing method thereof - Google Patents

Abstract

The present invention relates to fish cake containing non-denatured type II collagen, and more specifically, to fish cake using a composite including chicken sternal cartilage extract powder, which is a raw material of a composition for preventing or treating arthritis of Patent No. 10-1565159, and herbal materials as filling ingredients, and allowing fish paste dough and fish cake meat to protect the filling ingredients, and to a manufacturing method thereof. According to the present invention, it is possible to prevent a form and features of filling ingredients from being deformed due to edible oil of a high temperature being in contact with the filling ingredients made from collagen and herbal composite materials, thereby maintaining efficiency of non-denatured type II collagen and herbal composite materials and unique taste and texture of fish cake. The fish cake containing non-denatured type II collagen of the present invention is provided in a form of a food material good to chew, swallow, and digest as a method of allowing elderly people to intake non-denatured type II collagen and the herbal composite materials constantly and conveniently for health management and anti-aging. Also, as a popularly familiar food material, the fish cake of the present invention can be helpful for diet, skin care, and health.

Description

Technical Field [0001] The present invention relates to a fish cake containing a non-denatured type 2 collagen,

The present invention relates to chick chest cartilage extract powder having a preventive effect against aging and arthritis, and a raw material containing a herbal ingredient and a meat dough or fish paste flesh wrapped around the ingredient. More specifically, the ingredients include chicken chest cartilage extract powder, honey, It contains the ingredients, prevents the high-temperature edible oil from directly touching the ingredients, and uses the potato dough, which is highly effective as a food material, to be easily eaten by the public. The present invention relates to a method of manufacturing a fish paste which can be used as a fish paste.

Recently, lifestyle diseases such as diabetes and hypertension, degenerative diseases such as dementia, arthritis and arteriosclerosis, which are regarded as the exclusive items of the elderly population, are increasingly recognized as a general disease as the age of onset gradually decreases and becomes widespread. The desire to prevent aging and eliminate the early symptoms of degenerative diseases / early treatment and to live healthy without disease has become a research force in all fields.

From the biological point of view of cell level, the harmful oxygen and collagenase are considered to be the key factors of aging. The harmful oxygen attacks the cell membrane lipids and prevents the skin cells from being damaged by the damaged cell membranes. Increases in collagenolytic enzymes are related to arthritic disease by fiber degeneration and destruction, and appear to be an important cause of wrinkles.

Fish cake is a popular food that can easily ingest a low calorie high quality protein. Among various types and forms of fish paste, fish paste which includes other ingredients in fish paste has both fish paste and inner ingredients used in the shell.

Aging is one of the representative life phenomena, and it refers to all the physiological changes that the body undergoes over time. Many diseases such as cancer, cardiovascular disease, obesity and fatty liver are basically related to the deterioration of cells and individual organs and organs, and there is a deep discussion about whether this is regarded as a disease or aging process. .

As one of the representative diseases related to aging, 5% of the Korean population suffers from chronic arthritis, and a large number of elderly people who are not diagnosed with the disease name of arthritis suffer pain and discomfort related to joints. The direct cause of arthritis is not yet clear, but T cells play an important role in inflammatory mediators and local release of cytokines, resulting in a sustained autoimmune reaction that destroys the joints. Fatigue, helplessness, and pain, arthritis is accompanied by fever and physical weakness. In addition, muscle atrophy and muscle spasm appear near the inflamed joints, affecting movement of the joints.

Various therapeutic agents such as cortisone, corticosteroids, steroid drugs and non-steroidal anti-inflammatory drugs such as aspirin, pyroxycam and indymethacin have been used for the treatment. These chemotherapeutic agents have no fundamental therapeutic effect, they relieve pain and temporarily eliminate inflammation, but because they put a heavy burden on the stomach, it is difficult to take the amount needed for pain relief continuously for a long time.

Non-denatured type 2 collagen is highly absorbed up to the intestinal immune system by digestion in the gastrointestinal tract with little structural change of collagen. This non-modified type 2 collagen has a cartilage regeneration effect with collagen which occupies most of the cartilage constituent material except for 70 ~ 80% moisture. Natural products such as chickens chest cartilage extract powder, honey, onion, reed roots, goji berries, ganoderma, mint, gill root, purlins, corn oil, peanut roots and oak root roots have traditionally been treated for over a thousand years for arthritis and inflammation And has been used in practical disease treatment research with its effects and functions by recent medical researches.

The present invention relates to a composition comprising a natural composite material effective for various inflammatory diseases including arthritis and a composition of non-modified type 2 collagen closest to the collagen structure in the body, as a starting material, and using fish cake as a crust for protecting the ingredient, The natural products used in the present invention will be briefly described as follows.

Chicken sternal cartilage extract powder is a major constituent of collagen, which corresponds to the type 2 type of collagen. Collagen is one of the major constituent proteins of proteins, chondroitin sulfate, water, phosphorus, and ash. It is a major constituent of skin or articular cartilage and occupies a wide distribution of human tissues such as ligaments, bones, and blood vessels. It is classified into types 1, 2, 3 to 20 according to in vivo distribution or property. The major component of joints is type 2 collagen, except for water. Type 2 collagen acts as a lubricant for cartilage, and it plays a role in helping movements of joints by binding moisture in tissues, thereby relieving the pain of osteoarthritis and rheumatoid arthritis, and delaying cartilage regression of human joints. Reported. Collagen can be consumed as much as food, but collagen, which is a large molecule and consumed, is decomposed from the digestive tract into amino acids and collagen does not reach joints and cartilage tissues. Non-denatured type 2 collagen is recognized as a useful substance that reaches the small intestine in almost the form in the body and shows higher functionality than denatured collagen ingested in the form of pig shell or foot.

Honey has been used for health, beauty and medical purposes in most cultures for the last 2,500 years. It contains 69% glucose and fructose. It can be used as a sweetener which is healthier than white sugar. It helps recovery of blood glucose, recovery of muscle, and glycogen restoration after exercise. It contains high levels of minerals and vitamins. It has excellent antibacterial and antifungal properties and is used as a natural preservative.

Onion (Allium cepa, English onion), among the onion belonging to the leek, it is what we call garden onion, bulb onion, shallot in English. It has been known to have healing effects from ancient times and has been with humanity for over 5,000 years. Cold, asthma, bacterial infections, respiratory diseases, angina, and cough. I will. Even the World Health Organization (WHO) has confirmed that it is beneficial to people with reduced appetite and people with inflammatory symptoms. Chronic asthma, allergic bronchitis, common cough related to colds, and cold syndrome patients are effective in protecting the immune system and overcoming inflammation.

The reed roots (nogeun, hragmites australis, reed root in English) have recently been found to be powerful components for synthesizing acetylcholine, a neurotransmitter, and are being actively studied for use as aging inhibitors. Traditionally, it has been used for diseases such as vomiting, urinary system and arthritis. It is harvested in spring and autumn, dried, boiled or used as a medicine. It is effective against various inflammation with strong sterilizing action.

Lycium chinense (English name: Matrimony vine) has been known since ancient times as a fruit that regains energy and strength, and increases physical strength and longevity. It is the second most abundant source of vitamin C in plants and contains 21 essential minerals, including rare germanium, and 18 amino acids. It is about five times the level of antioxidants found in major plants known as superfoods. Some anti-inflammatory ingredients, essential fatty acids such as linoleic acid to help reduce body fat, polysaccharides that contribute to heart and blood pressure maintenance, and growth hormones and anti-bacterial compounds that help maintain the balance of the immune system.

The fruit of the genus Jupiter (Scientific name Actinidia polygama, English silver vine) has been used for centuries as a preventative health supplement in Asia for hypertension and alternative therapies for arthritis pain. Vitamin C is 10 times higher than green tea and is much higher than fruit varieties such as strawberries, kiwi, blueberries and lemons. The antiinflammatory properties of giardia officinalis, which is used to treat rheumatoid arthritis and alleviate arthritis pain, are used in lung inflammation and anticancer studies.

Mint (Menthae canadensis, English Menthae Herba). Helps in respiratory health and digestion. It is also used to treat various syndromes due to hormonal imbalance. It promotes red blood cell and hemoglobin production with high iron content and improves energy regal and wound healing ability. Limonene, cineol, pinene, vitamin A, vitamin C, riboflavin and thiamine, and antioxidants are powerful tools to prevent chronic diseases, including cancer, caused by oxidants that attack healthy cells.

Valeriana fauriei is a relative plant belonging to the same genus. It has a small pupa, broad leaf pupa, and a giant pupa. They are all used as medicinal materials and have a peculiar smell. From the second century on, it was widely used in Europe for the treatment of anxiety and neurological conditions. Recently, many studies have been made as one of the medicines for improving the quality of sleep, and they are also used for altitude sickness, menstrual irregularities, stomach cramps, arthritis, bruises. Pharmacological actions have been reported for sedation, antipyretic action, hypotension, inhibition of Gram-positive bacteria, and diuretic action.

Pueraria lobata (Puerariae radix) is a bark of roots and has no smell. It has been used in Asia for over 1000 years as a maternal herb to reduce heat and remove toxic substances from the body. It directly inflates blood vessels and reduces peripheral resistance to lower blood pressure and alleviate the rigid symptoms of hypertension. Recent studies have shown that they have anti - cancer and estrogen - like effects and can be used as natural breast augmentation and cosmetic products, which is effective for middle - aged women and menopausal women.

Cornus officinalis (Cornus officinalis) Cornus officinalis (Cornus officinalis), when used with other medicines fruit is known to strongly expose the efficacy of the drug itself and the relative. Used for the treatment of diabetes, arthritis and impotence. It can also be used to strengthen the knee, to control body fluids, or to treat menstrual irregularities. The astringent obtained by boiling the bark is used as a fever and malaria inhibitor.

Peony root (scientific name Paeonia lactiflora, English Peony Root) has been used as food and medicine in Asia for centuries because of its high nutritional value. The roots are known to have excellent antibacterial, antifungal, antiallergic, analgesic, anticoagulant and sedative effects. Flavonoids, tannins, proanthocyanidins, asparagines, sitosterols, glycosides and monoterpenes. Muscle relaxation, skin health, vascular injury and inflammation, and prevention of liver inflammation.

Brewer's yeast contains 1 (thiamine), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxine), B9 (folate), H or B7 (biotin). These vitamins help break down carbohydrates, fats and proteins that supply energy to the body. Supports the nervous system, maintains the muscles used for digestion, and contributes to keeping the skin, hair, eyes, mouth and liver healthy. By lowering the blood sugar level, it improves the glucose tolerance and helps the management and treatment of diabetes. It also helps to lose weight by lowering the cholesterol level.

The root of the eucalyptus tree (Scientific name Eleutherococcus sessiliflorus, English eleuther) has been used medicinally in Asia for thousands of years and has become a more widely known medicinal plant in western Europe over the past 100 years. It strengthens the immune system and reduces various symptoms and inflammation reaction in the early stage of disease. It stimulates the metabolism and reduces the fatigue and improves the activity and energy level of the nervous system. In recent studies, it has been reported that the root of the oak tree reduces the pain of the infected area, which is a scientific basis for traditional use that is effective for inflammatory diseases such as osteoarthritis and gout.

As described above, the composition containing the composite material of the natural herbal composite material and the chest chest cartilage extract powder has a very high absorption rate of collagen than the widely known chicken legs, pig shells, and foot pads, and thus is effective for prevention of osteoarthritis and cartilage regeneration. It can be cooked variously, and it can be enjoyed deliciously in a healthy and high-protein food form of low calories.

It is an object of the present invention to provide a fish paste including the natural herbal composite material and the chest chest cartilage extract powder and a method for producing the same.

Collagen is composed of glycosaminoglycans (GAGs or mucoadas glycans) and proteins. It is a three-dimensional linear structure with a negative charge. It acts as a transport medium of mineral such as magnesium, calcium, phosphorus, It is involved in bone metabolic function. It is especially important in the organelles, the cancellous bone, and acts to defend inflammation against viruses or bacteria for intracellular stabilization. The cartilage of each joint in the body consists of about 60-80% water, 15-20% collagen, 3-5% glucosamine and chondroitin, and hyaluronic acid.

Although it is known that it is abundantly contained in chicken legs, pig shells, and paws among general foods, most of them are decomposed and absorbed into amino acid level during digestion process, and only very few of them are used for collagen synthesis. Combined with a natural need for anti-aging and information on foods that are known to have high collagen content, it is recommended to take only one serving daily. High calorie foods such as high-calorie chickens, pig shells, and paws that go far beyond calories are recommended for obesity The paradoxical situation that leads to this is everyday.

In order to solve the present paradoxical situation of consuming low-efficiency collagen through foods that induce obesity, Chicken Chest Cartilage Powder as a main material of non-denatured type 2 collagen which has been found to have the highest absorption rate in the body among natural edible collagen, Lt; / RTI > It uses a high-protein low-fat fish paste (fish paste) as a method to protect and conveniently ingest a natural herbal composite which helps absorption and regeneration of non-modified type 2 collagen and body collagen.

According to the present invention, by eating high quality non-denatured type 2 collagen and pharmaceutical composition in the form of fish cake, it is possible to enjoy convenient and delicious food for health without inducing obesity such as chicken legs, pig shells or foot paws.

A cross-section of a fish cake made of fish cake containing a non-modified type 2 collagen and ingredients to protect the ingredients
1 - Ingredients: Chicken chest cartilage extract powder in potato or potato mousse, Composition kneaded with natural herbal composite powder and honey
2 - Crust: fish paste made from fish paste

In order to achieve the object of the present invention, the chest chest cartilage extract powder and the dried powder of onion, reed roots, ginger fruit, ganoderma lucidum, peppermint leaves, Prepare them separately and mix them with mashed mush or mashed steamed sweet potatoes, and then add honey bees to form the kneaded mixture of natural herb complexes into a sticky bar shape. The ingredients thus prepared were measured for activation of hyaluronidase, skin elasticity test and skin wrinkle inhibition test were conducted by human body test, and the bone mineral density and elasticity of the experimental animals were measured and observed. As a result, It was confirmed that the symptoms of arthritis can be alleviated.

The present invention relates to a composition comprising chickens chest cartilage extract powder and honey, and dried powder of onion, reed roots, ginger fruit, ganoderma powder, mint leaf, purlins, gill root, corn oil, peony root, brewer's yeast, And a potato mousse or sweet potato mousse containing the composition as a starting material, and the ingredients are fully sealed with a fish paste and then cooked, roasted or fried to provide a foodstuffed fish paste.

In the present invention, the chick breast cartilage extract powder used in the ingredients of the fish paste according to the present invention and onion, reed roots, goji berries, ganoderma lucidum, peppermint leaves, pruning roots, The composition consisting of roots provides a food composition for improving anti-aging and joint inflammation.

In the present invention, the composition of the ingredients of the fish paste except for the chewing gum mousse or steamed sweet potato mousse contains 10 to 40% by weight of the chest chest cartilage extract powder, 1 to 20% by weight of honey, 1 to 10% by weight of onion, 1 to 10% 5 to 15% by weight of ganoderma powder, 5 to 15% by weight of ganoderma powder, 1 to 10% by weight of mint leaf, 1 to 10% 5 to 10% by weight of peanut root, 1 to 10% by weight of brewer's yeast, and 5 to 10% by weight of root root.

[Example]

Hereinafter, preferred embodiments of the present invention will be described. The following examples are provided to aid understanding of the present invention, but the present invention is not limited to the following examples.

<Examples 1 to 5> Preparation of Ingredients for Anti-Aging and Joint Inflammation Treatment According to the Present Invention>

1 to 10 wt% of onion, 1 to 10 wt% of reed roots, 5 to 20 wt% of ginger root, 5 to 15 wt% of ginkgo nut, 1 to 10 wt% of mint leaves, 1 to 10 wt% of ginger root, Dried pellets of 5 to 10 wt.%, Gill root 5 to 15 wt.%, Corn oil 5 to 10 wt.%, Peanut root 5 to 10 wt.%, Brewer's yeast 1 to 10 wt. And homogenously mixed. Then, 10 to 40% by weight of chest chest cartilage extract powder was added and mixed homogeneously. The thus prepared mixed powder was cooled, and the resulting mixture was put in a mashed mousse. The mixture was mixed with 100% by weight of a mixed powder in a ratio of 100% by weight, and finally mixed with 1 to 20% by weight of honey to prepare a starting material.

Honey is more or less than the ratio shown in the above Table 1 in order to maintain the viscosity of the starting material itself properly for the purpose of facilitating the molding of the entire material and preventing the deterioration of the ingredients in the distribution process due to the sterilization and antibacterial action peculiar to honey Which is influenced by the humidity and temperature of the blade to be manufactured.

The prepared ingredients were foamed in soy sauce oil at 150 ° C to 180 ° C, wrapped completely in a hermetically sealed kelp flesh in the form of dough. At this time, the time was shortened when compared to the case of frying a complete fish cake of general 100% fish paste to minimize the heat applied to the ingredients.

&Lt; Comparative Example 1 &

The same procedure as in Example 1 was carried out except that 100% by weight of the mixed powder was kneaded to 200% by weight of mousse and 1 to 20% by weight of honey were mixed and mixed.

&Lt; Comparative Example 2 &

The same procedure as in Example 1 was carried out except that 100% by weight of the mixed powder was kneaded to 300% by weight of mousse and 1 to 20% by weight of honey were mixed and mixed.

&Lt; Comparative Example 3 &

The same procedure as in Example 1 was carried out except that 100% by weight of the mixed powder was kneaded into 400% by weight of mousse and 1 to 20% by weight of honey was mixed and mixed.

&Lt; Evaluation 1: Change in viscosity of the ingredient according to the composition ratio of the ingredients &lt; RTI ID = 0.0 &gt;

Composition of Ingredients in the same Moist Weight The change of the powder component ratio does not have a great influence on the viscosity of the finished material itself, and when the honey does not enter at all, the ingredient material tends to become rough, It was found that honey should be absolutely included even for sterilization and distribution safety as well as visual completeness of the finished product.

<Evaluation 2: Variation of flavor of finished product according to weight change of sweet potato mousse>

The product according to the present invention has a characteristic flavor of the herbal medicine material in the nature of the ingredient. In the case of Example 1, the clear herbal medicine flavor and the potato flavor are felt together with the finished product, but the aroma of the herb medicine is lessened as the content of the mousse is higher I could. The amount of non-modified 2-type collagen and herbal composite materials included in the single dose should be further examined and calculated. However, considering the purpose and efficacy of the present invention, It is also useful to let people who eat fish cake by hand recognize that it is a special product.

EXPERIMENTAL EXAMPLE 1 Measurement of Inhibition of Hyaluronidase Activity of Component Material Composition According to the Invention [

Hyaluronic acid is a substance contained in many cartilage tissues in vivo, plays a major role in improving the elasticity of cartilage tissue, has high moisture content, and has excellent viscoelasticity. In general, accelerated degradation of hyaluronic acid is observed in osteoarthritis patients. There are reports that hyaluronidase and the like increase the cell death of articular cartilage cells and decrease the metabolism of cartilage cells to promote degenerative changes of cartilage tissue. Therefore, inhibition of hyaluronidase, which is a degradative enzyme of hyaluronic acid, is thought to be a therapeutic effect of osteoarthritis.

In order to examine the hyaluronidase activity inhibition of the mixture of the present invention, the activity was measured using the Morgan-Elson Assay method. Hyaluronic acid dissolved in phosphate buffer solution and the extract mixture prepared in the above example dissolved in DMSO were added to each well and cultured at 37 ° C for 20 minutes. After incubation, the compound 48/80 solution was added and secondary cultured. After hyaluronic acid was added and cultured for the third time, K3BO3 was added and the reaction was stopped by heating in hot water. After centrifugation, N-acetylglucosamine, which is produced by degradation of hyaluronic acid by hyaluronidase, was quantified by colorimetry at 585 nm using DMAB (p-dimethylaminobenzaldehyde), and its activity was verified. Celecoxib (Pfizer) was used as a positive control.

The results are shown in Table 2.

As shown in Table 2, in Example 3 of the mixture of the present invention, the activity inhibition of hyaluronidase was 63.9 ± 0.47% and the inhibition of positive control celecoxib activity was 62.7 ± 0.38, And the inhibition effect was high.

Experimental Example 2 Skin Elasticity Test of Ingredient Composition According to the Present Invention [

Skin elasticity effects were measured with the mixtures prepared according to Examples 1 and 3. The measurement method was as follows: 10 healthy women without skin disease were divided into left and right crows feet at 25 ° C, 50% relative humidity and room temperature and humidity conditions. 1, on the right side, Example 3 was divided into small portions of 8 grams of mung bean egg size per day for 4 weeks, and divided into 30 minutes before breakfast. The elasticity of the skin was measured by Cutometer MPA580 (Courage and Khazaka, Kiln, Germany).

The R2 value in the measuring equipment means the gross elasticity of the skin due to the ability to re-deform the skin. In Example 1, the R2 value was increased by 13.6% in the measurement result, and in Example 3, the R2 value was increased by 18.9% in the measurement result, and the skin elasticity was increased.

<Experimental Example 3: Skin wrinkle test of ingredient material composition according to the present invention>

The wrinkle effect of the skin was measured with the mixture prepared in Example 3. The subjects were 10 healthy women without skin disease at 25 ℃, 50% relative humidity and room temperature and humidity condition. The subjects were divided into left and right eyes at the crows feet, The rats were divided into two groups of 8 grams of green bean sprout sized for 6 weeks and divided into two groups and fed 30 minutes before breakfast. And then analyzed for skin wrinkles by performing image analysis on replica skin using Visiometer (Skin-Visiometer SV 600, Courage & Khazaka, Germany).

R3 value means Average Roughness. In other words, it is a direct parameter related to the depth of wrinkles. Therefore, it was observed that there was a difference in the wrinkle-improving effect because the difference between -0.05 and -0.051 in the R3 value was decreased from 6 weeks (0.181) to 0 week (0.232).

EXPERIMENTAL EXAMPLE 4: Animal Effect Test of Ingredient Composition According to the Present Invention [

The following experiment was conducted to confirm the effect of the elasticity of the bone mineral density and the bone bioindex of the mixture of the present invention.

As experimental animals, 10 rats weighing 240-260 g are randomly divided into two groups of 5 rats each. A negative control (ovariectomized rats, protein containing 4000 mg / kg calcium base) and a test group (ovariectomized rats, 10 g / kg feed of Example 3). After an adaptation period of one week, anesthetize with 2% sodium pentobarbitol solution by intraperitoneal injection and excision of both ovaries. Two days after the operation, the vaginal test is performed. After 5 days, the vaginal interval is checked and the success is confirmed. (1 g / kg of protein containing 4000 mg / kg of calcium) in a negative control for 8 weeks after one week of operation, and 10 g / kg of the mixture of Example 3 in the test group. After 8 weeks of testing, the mice are dissected and the surrounding thigh tissue is removed to fill the right femur and tibia. And keep it at minus 70 degrees. The tibia is kept in gauze impregnated with physiological saline solution. Test bone density and elasticity after 1 day.

The bone mineral density of the thigh bone was increased by 3.66% in Example 3 (1.245 ± 0.037) compared to the control (1.201 ± 0.051), and elasticity was 38.57% elasticity in Example 3 (425.7 ± 238.1) compared to the control (307.2 ± 59.7) And the effect is shown on the joints.

As described above, the present invention provides chickens chest cartilage extract powder and a herbal composite material, which are core raw materials of non-modified type 2 collagen, in the form of fish paste, which is a popular food for easy and convenient ingestion in daily life, It is a new healthy wellbeing food in the fine fish paste market. It is effective for prevention of aging, skin abuse, and health care. It is a useful invention contributing to the promotion of public health.
The detailed description of the invention is merely exemplary of the invention, which is used for the purpose of describing the invention only, and is not used to limit the scope of the invention as defined in the claims or the claims.
It is therefore to be understood that various modifications and equivalents may be resorted to by those skilled in the art.
Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (3)

Composition which is used as a raw material of fish paste in which non-modified type 2 collagen and herbal composite material are mixed with an additive material such as rice flour or rice flour paste, sweet potato or potato mousse to form a food material A fish paste characterized by using the composition of claim 1 as a carrier material and using meat or fish meat as a shell for protecting the ingredients and providing a taste and texture of the fish paste The composition of claim 1, which is a pharmaceutical composition,

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