KR20190000175A - Cosmetic composition for preventing uv-induced skin damage comprising the extract of solidago virgauea l. var coreana nakai - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising an extract of Solidago virgaurea as an active ingredient. The cosmetic composition prevents skin cells from being damaged by UV rays, prevents the generation of active oxygen, and promotes the growth of cells damaged by UV rays. According to the present invention, the extract of Solidago virgaurea absorbs UV B, prevents damage to skin cells from UV B, and promotes the growth of cells damaged by UV B. In addition, the extract of Solidago virgaurea of the present invention prevents the generation of active oxygen induced by UV B.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for protecting skin against ultraviolet rays,

The present invention relates to a cosmetic composition which inhibits skin damage and production of active oxygen caused by ultraviolet rays containing an undiluted extract as an active ingredient.

The skin is divided into three layers of epidermis, dermis and subcutaneous fatty tissue in order from the outside, and functions to protect the human body from physical and chemical stimulation of the external environment. The most important environmental factors affecting the protective function of the skin are ultraviolet rays. The ultraviolet rays irradiated to the skin increase the generation of free radicals and reactive oxygen species (ROS) in the skin , DNA, cell membranes and proteins, leading to skin aging and inflammatory lesions of the skin.

Ultraviolet rays are divided into ultraviolet rays C (200 to 280 nm), ultraviolet rays B (280 to 320 nm) and ultraviolet rays A (320 to 400 nm) depending on wavelengths. Ultraviolet rays reaching the surface and affecting the human body are ultraviolet rays A and B It is known.

In particular, ultraviolet B induces oxidative stress by inducing the production of reactive oxygen species (ROS) such as hydroxyl radicals and superoxide anion, disrupting the antioxidant defense system, Not only induces inflammation, but also deepens diseases such as inflammation, photoaging and skin cancer.

Therefore, in order to protect skin cells from ultraviolet rays and prevent aging, it is important to strengthen the antioxidant defense system capable of effectively suppressing oxidative stress caused by active oxygen and efficiently removing active oxygen.

Therefore, recently, research on natural substances which are harmless to human body and have more powerful antioxidative effect has been actively carried out, and it is attracting attention as a strategy for protecting skin from ultraviolet rays.

There have been a number of studies to examine whether known antioxidants can be used as skin protectants against skin damage due to exposure to ultraviolet light. Carotenoids, alpha-tocopherol, alpha-lipoic acid and astaxanthin have O ₂ quenching activity (-) - epigallocatechingallate belonging to catechins, curcumin belonging to cucuminoids, resveratrol belonging to stilbenoids, gallic acid belonging to tannins, ascorbic acid belonging to tannins, ascorbic acid, coenzyme Q10 and polyphenols such as catechin, Sesamines belonging to the league turbulence, and pyrocatechol, caffeic acid and pyrogallol have been reported to exhibit no or very weak O ₂ scavenging activity (Carotenoid Science, Vol.11, 2007, 16-20).

There are few studies that can effectively inhibit skin damage due to exposure to ultraviolet rays. Especially, it has been confirmed that there are few studies related to natural extracts having excellent skin protection effect with few side effects.

Therefore, development of a new cosmetic material which suppresses the production of active oxygen by exposure to ultraviolet rays and is excellent in protection against skin damage has been desired.

On the other hand, Solidago virgaurea L. var coreana Nakai is a perennial plant belonging to the family Asteraceae, which is a perennial herbaceous plant. It grows beneath the mountain forests of the whole country and is distributed geographically in Japan, Manchuria, China, Taiwan, and the Philippines.

It has a unique taste and flavor and is mainly used for food. It has a pharmacological effect and its outposts are called "one branch 黄花", which is used as medicines for cold, headache, pain, dryness, kidney, pneumonia. The main ingredients are Caffeic acid, Quercetin, Rutin, Astragalin, Chlorogenic acid, Solidagosaponin, Limonen, Bornylacetate, Borneol.

Regarding the cosmetic material research using such a non-sterilizing agent, recently, a cosmetic composition containing an extract of Narakami staple, anchovy, and zoospora of Korean Patent No. 10-1528275 has been proposed.

Accordingly, the inventors of the present invention have found that the extract of the present invention is excellent in skin cell damage and active oxygen production inhibition effect by ultraviolet rays, and also has a regenerating effect of skin cells damaged by ultraviolet rays, thereby being useful as cosmetic composition for skin protection against ultraviolet ray exposure And thus the present invention has been completed.

KR 10-1528275 B1

 Carotenoid Science, Vol.11, 2007, 16-20

It is an object of the present invention to provide a cosmetic composition which inhibits damage of skin cells caused by ultraviolet rays containing an undiluted extract.

Another object of the present invention is to provide a cosmetic composition which promotes the proliferation of skin cells damaged by ultraviolet rays containing an undiluted extract.

It is still another object of the present invention to provide a cosmetic composition which inhibits the production of active oxygen by ultraviolet rays containing an undiluted extract.

In order to achieve the above object, the cosmetic composition of the present invention is characterized in that it protects skin from ultraviolet rays and contains an undiluted extract as an active ingredient.

The above-mentioned non-averted extract absorbs ultraviolet ray B, inhibits skin cell damage induced by ultraviolet ray B, and promotes the proliferation of skin cells damaged by ultraviolet ray B.

The above-mentioned non-microorganism extract is characterized in that production of active oxygen induced by ultraviolet ray B is suppressed.

Wherein the extract is an extract obtained by using water, an organic solvent or a mixed solvent thereof, and the organic solvent is at least one selected from the group consisting of alcohols having 1 to 5 carbon atoms.

Characterized in that the non-dried extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a cosmetic composition which contains, as an active ingredient, an undesirable extract, inhibits skin cell damage and active oxygen production by ultraviolet rays, and promotes proliferation of cells damaged by ultraviolet rays. It is possible to satisfy consumers' demand for a cosmetic composition derived from a small natural product.

1 is a graph showing the results (ultraviolet ray B absorption efficiency) of Experimental Example 1 according to the present invention.
2 is a graph showing the results (cytotoxicity) of Experimental Example 2 according to the present invention.
3 is a graph showing the results (antioxidant effect) of Experimental Example 3 according to the present invention.
4 is a graph showing the results of Experimental Example 4 according to the present invention (inhibitory effect on active oxygen production in cells).
Fig. 5 is a graph showing the results of Experimental Example 5 according to the present invention (skin cell damaging inhibitory effect). Fig.
6 is a graph showing the results (skin cell regeneration promoting effect) of Experimental Example 6 according to the present invention.

Hereinafter, the present invention will be described in detail.

The cosmetic composition according to the present invention protects the skin exposed to ultraviolet rays. The protection of the skin means that damage to skin cells induced by exposure to ultraviolet rays is suppressed and the production of active oxygen is inhibited. It also includes promoting regeneration of skin cells damaged by ultraviolet light.

In order to protect the skin from ultraviolet rays, the present invention provides a cosmetic composition comprising an unstable extract as an active ingredient. The unstable extract has no irritation to the skin and has excellent stability of the product after formulation, Is suitable as a composition.

In order to protect the skin from ultraviolet rays, it is preferable that the anhydrous extract is contained in an amount of 0.01 to 10% by weight in the cosmetic composition. If the concentration is too low, skin protection effect against ultraviolet rays is insignificant. There is a disadvantage that the manufacturing cost is increased without showing the protective effect. In view of the skin protection effect and the production cost, it is most preferably contained in the range of 0.05 to 0.1% by weight.

The extract may be in a liquid form, and may be prepared in powder form through an additional process such as extraction, concentration under reduced pressure, freeze drying, or spray drying. Of course.

Hereinafter, the method for preparing the above-mentioned undiluted extracts will be more specifically described.

The extract may be obtained by extracting and isolating from nature using an extraction and separation method known in the art, and the extract is extracted from undrawn with an appropriate solvent. The extract is prepared by extracting hot water, polar solvent-soluble extract Or non-polar solvent-soluble extracts.

The type of the solvent is not limited, and any solvent acceptable in the art may be used, and water or an organic solvent may be used as an example. The organic solvent may be at least one kind of alcohol having 1 to 5 carbon atoms including methanol, ethanol, propanol, isopropanol, and butanol. It is possible to use at least one of organic solvents in combination, but there is no need to be limited thereto.

The extraction method may be selected from the group consisting of a hot water extraction method, a cold extraction extraction method, a reflux cooling extraction method, a solvent extraction method, a steam distillation method, an ultrasonic extraction method, a leaching method and a pressing method.

As an example of the above extraction method, the undissolved product is pulverized, and at least one extraction solvent selected from water having a dry weight of 1 to 50 times by volume and an alcohol having 1 to 5 carbon atoms is added, and the solution is dried at 4-30 DEG C for 2-7 days Followed by stirring to extract an active ingredient, which is then filtered and concentrated by a reduced pressure concentrator. In addition, it can be prepared as a powder by lyophilizing if necessary.

At this time, the above-mentioned undesirable grains can be used as it is, but it is usually dried and used. The drying method can be natural drying method such as shade. The above-mentioned pulverization particle size can be easily extracted, for example, about 10 to 100 mesh, and is not limited thereto. In addition, filtration, concentration under reduced pressure, freeze-drying and the like may be satisfactorily carried out according to a generally known method.

In addition, the cosmetic composition of the present invention may contain any conventional ingredients other than the above-mentioned effective ingredients, including conventional additives such as diluents, excipients, buffers, dispersants, surfactants, colorants, perfumes or sweeteners can do. In addition, the cosmetic composition may further include a functional cosmetic ingredient, a moisturizer, and other additives that impart a cosmetic function to the skin, and may further include at least one cosmetically acceptable carrier incorporated in a general skin cosmetic composition . Examples of the carrier component include animal oils, vegetable oils, waxes, paraffin, silicone, lactose, silica, talc, water, ethanol, benzyl alcohol, glycerol aliphatic ester and aliphatic alcohol sulfuric acid. Of course.

The cosmetic composition according to the present invention may also be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray , But is not limited thereto.

In addition, the formulation examples are the same as those of the conventional cosmetic composition, except that the undiluted extract of the present invention is added.

Hereinafter, the present invention will be described in more detail with reference to the following experimental examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example  One: Undisturbed  Preparation of extract

The undiluted outposts were thoroughly cleaned, shredded and shredded to about 100 mesh using a pulverizer. Then, 200 ml of 80% ethanol was added to 20 g of the crushed unmealized sample, followed by stirring at 20 ° C for 72 hours. The mixture was filtered through a filter paper, concentrated under reduced pressure using a rotary evaporator, and lyophilized to obtain a powdery extract.

Experimental Example  1: ultraviolet ray Absorption capacity  exam

The following experiment was carried out to confirm the ultraviolet absorbing effect of the anthocyanin extract according to the present invention.

Specifically, the unlabeled extract prepared in Example 1 was measured for its optical density in a spectral range of 280 to 700 nm using a UV / Vis spectrophotometer. The results are shown in Fig. At this time, the undiluted extract was diluted to a concentration of 100 ppm, and 80% ethanol was used as a diluting solvent.

As shown in FIG. 1, the extract of Example 1 according to the present invention showed high absorbance in the region of ultraviolet ray B (280 to 320 nm).

From this, it was found that the skin cells can be protected against ultraviolet B exposure by containing an extract of anisostea as an active ingredient at a concentration of about 100 ppm.

Experimental Example  2: Cytotoxicity test

The following experiment was carried out to confirm whether or not the anhydrous extract according to the present invention had a direct effect on skin cell death.

Specifically, HaCaT cells were inoculated into a 96-well plate at a concentration of 1.0 × 10 ⁴ per well using DMEM (in 10% FBS, 1% Penicillin / streptomysin) as a medium. After 24 hours, The resulting anhydrous extract was treated to the culture medium by concentration. After 72 hours, the MTT reagent was treated with 100 μl of each well, incubated at 37 ° C. in a CO ₂ incubator for 4 hours, and the optical density was measured at 570 nm using an ELISA. The results are shown in Fig.

As shown in FIG. 2, the extract of Example 1 according to the present invention was found to have no direct effect on skin cell death up to a concentration of 100 ppm.

Experimental Example  3: Antioxidant test

The following experiment was conducted to confirm the antioxidant activity of the anthocyanin extract according to the present invention.

Specifically, 5 ml of a solution of 7 mM ABTS (2,21-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) and 5 ml of a 2.6 mM potassium persulfate solution were mixed and shaded for 12 hours. The ABTS free radical solution was added in an amount equivalent to 50 μl of the solution according to the concentration of the unlabelled extract obtained in Example 1, and the reaction was carried out at room temperature for 10 minutes in the light-shielded state Then, the optical density was measured at 517 nm using an ELISA. Ascorbic acid was used as a positive control, and the results are shown in FIG.

As shown in FIG. 3, the extract of Example 1 according to the present invention was found to have excellent ABTS radical scavenging activity. From these results, it was found that the antioxidant activity was increased about 48% by adding the extract of Seoren Seed Extract as an active ingredient at a concentration of about 10 ppm.

Experimental Example 4: Inhibition of active oxygen production in cells

The following experiment was conducted to confirm the efficacy of inhibiting the production of intracellular active oxygen induced by ultraviolet B irradiation of an undiluted extract according to the present invention.

Specifically, HaCaT cells were inoculated into a 96-well black plate at a concentration of 1.0 × 10 ⁴ per well using DMEM (in 10% FBS, 1% Penicillin / streptomysin) as a medium. After 24 hours, the medium was removed and the DCFH-DA (2 ', 7'-Dichlorodihydrofluorescin diacetate) reagent was pretreated for 30 minutes. DCFH-DA (2 ', 7'-Dichlorodihydrofluorescin diacetate) reacts with active oxygen in the cell to produce a fluorescent substance (2', 7'-Dichlorodihydrofluorescein: DCF) Active oxygen can be measured.

Next, after the DCFH-DA reagent was removed, 50 μl of PBS buffer was treated for each well, and the unlabeled extract obtained in Example 1 was treated for each concentration. After irradiation of ultraviolet ray B at a dose of 30 mJ / cm 2, the PBS buffer was removed, and the medium was treated with 100 μl per each well, and the undiluted extract obtained in Example 1 was treated by concentration. After incubation for 2 hours, fluorescence was measured at excitation 480nm / emission 530nm using a microplate fluorometer. Ascorbic acid was used as a positive control. The results are shown in Fig.

As can be seen from the attached FIG. 4, the extract of Example 1 according to the present invention was found to have an excellent inhibitory effect on active oxygen production in cells. From this result, it was found that the production of intracellular active oxygen induced by ultraviolet ray B was reduced by about 37% by containing the extract of Echinodermus as an active ingredient at a concentration of about 100 ppm.

Experimental Example  5: skin cell damage inhibition test

The following experiment was conducted to confirm the effect of inhibiting skin cell damage induced by ultraviolet B irradiation of an undiluted extract according to the present invention.

Specifically, HaCaT cells were inoculated into a 96-well plate at a concentration of 1.0 × 10 ⁴ per well using DMEM (in 10% FBS, 1% Penicillin / streptomysin) as a medium. After 24 hours, the medium was removed. Then, PBS buffer was treated with 50 μl of each well, and then the unlabeled extract obtained in Example 1 was treated by concentration. After irradiation with ultraviolet ray B at a dose of 30 mJ / cm 2, the PBS buffer was removed, and the medium was treated with 100 μl of each well. Then, the undiluted extract obtained in Example 1 was treated by concentration.

After incubation for 24 hours, 50 쨉 l of the LDH reagent was treated with 50 쨉 l of the medium, left for 30 minutes at room temperature, and then the optical density was measured at 490 nm using ELISA. Ascorbic acid was used as a positive control. The results are shown in Fig.

As shown in FIG. 5, the extract of Example 1 according to the present invention was found to have excellent skin cell damaging activity. From this, it was found that the skin damage induced by ultraviolet B irradiation was reduced by about 69% by incorporating the anthocyanin extract as an active ingredient at a concentration of about 100 ppm.

Experimental Example  6: skin cell proliferation promotion test

The following experiment was conducted to confirm the effect of promoting the proliferation of damaged skin cells by irradiation with ultraviolet ray B of the anthocyanin extract according to the present invention.

Specifically, HaCaT cells were inoculated into a 96-well plate at a concentration of 1.0 × 10 ⁴ per well using DMEM (in 10% FBS, 1% penicillin / streptomysin) as a medium. After 24 hours, The PBS buffer was treated with 50 쨉 l per each well, and the unlabeled extract obtained in Example 1 was treated by concentration. After irradiation with ultraviolet ray B at a dose of 30 mJ / cm 2, the PBS buffer was removed, and the medium was treated with 100 μl per each well. The extract of the averages obtained in Example 1 was treated by concentration.

After 24 hours, the MTT reagent was treated with 100 μl of each well, incubated at 37 ° C. in a CO ₂ incubator for 4 hours, and the optical density was measured at 570 nm using ELISA. Ascorbic acid was used as a positive control. The results are shown in Fig.

As shown in the attached FIG. 6, the extract of Example 1 according to the present invention was found to have an excellent cell proliferation promoting effect. From this, it was found that the proliferation of the damaged cells was increased by about 16% by irradiating with ultraviolet ray B, by adding the anion extract to the active ingredient at a concentration of about 50 ppm.

Experimental Example  7: Material stability test

The following experiments were carried out to confirm the stability of raw materials of the anthocyanin extract according to the present invention.

Specifically, the anhydrous extract obtained in Example 1 was prepared as a solution having a concentration of 10,000 ppm, and stored for 3 months at 25 ° C., 42 ° C. and a cycle (0 ° C. → 25 ° C. → 40 ° C. for 8 hours each) , pH change and microbial contamination were measured.

As a result, it was confirmed that the anhydrous extract of Example 1 was stable for 3 months under all storage conditions without aggregation, sedimentation, discoloration, alteration, microbial contamination and pH change. Thus, it was confirmed that the stability of the undiluted extract according to the present invention was high.

Formulation Example  1: Lotion

The lotion containing the above-mentioned Example 1 was prepared by the following method with the contents shown in Table 1 below.

Materials No. 2, No. 3, No. 4 and No. 8 were sequentially added to the reaction vessel and dissolved by stirring. Subsequently, the No. 5 substance was dissolved by heating at about 60 ° C. and then added to the No. 9 material. Finally, substances No. 1, No. 6 and No. 7 were added to the No. 9 substance, and the mixture was thoroughly agitated, followed by aging at 25 ° C for 3 days to prepare a lotion.

Composition ratio of Formulation Example 1 number Raw material Content (% by weight) One The extract of Example 1 1.0 2 glycerin 3.0 3 Butylene glycol 2.0 4 Propylene glycol 2.0 5 Polyoxyethylene hardened castor oil 1.0 6 ethanol 10.0 7 Triethanolamine 0.1 8 Phenoxyethanol 0.0001 9 Purified water Balance

Formulation Example  2: Nutrition Lotion

The nutritional lotion containing the above-mentioned Example 1 was prepared in the following manner in the contents of Table 2 below.

Materials 10, 11, 13, and 14 were heated to 80 to 85 ° C while mixing and stirring. Subsequently, the materials 2, 3, 4, 5, 6, 7, 8, 9 and 12 were heated and dissolved at 80 to 85 ° C while being mixed and stirred, and then emulsified in mixing blends 10, 11, 13 and 14. After the emulsification was completed, the mixture was cooled to 35 ° C with stirring using an agitator, the material No. 1 was added thereto, cooled to 25 ° C, and aged at 25 ° C for 3 days to prepare nutritious lotion.

Composition ratio of Formulation Example 2 number Raw material Content (% by weight) One The extract of Example 1 1.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbitan stearate 1.0 7 Pro-type glycerin monostearate 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 Phenoxyethanol 0.0001 14 Purified water Balance

Formulation Example  3: Serum

The serum containing the above Example 1 was prepared in the following Table 3 in the contents.

Materials 2, 3, 5 and 8 were heated to between 40 and 45 ° C while mixing and stirring, and materials 4, 6 and 7 were added thereto for emulsification. After the emulsification was completed, the mixture was cooled to 35 ° C with stirring using an agitator, the material 1 was added thereto, cooled to 25 ° C, and aged at 25 ° C for 3 days to prepare a serum.

Composition ratio of Formulation Example 3 number Raw material Content (% by weight) One The extract of Example 1 1.0 2 Xanthan gum 0.02 3 Butylene glycol 10.0 4 Phospholipid 10.0 5 Hydroxyethyl cellulose 0.1 6 Sodium hyaluronate 5.0 7 Phenoxyethanol 0.0001 8 Purified water Balance

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

A cosmetic composition for skin protection against ultraviolet rays, characterized by containing an undiluted extract as an active ingredient.
The method according to claim 1,
Said non-averted extract absorbs ultraviolet ray B,
Inhibits skin cell damage induced by ultraviolet ray B,
And promoting the proliferation of the skin cells damaged by the ultraviolet ray B. The present invention relates to a cosmetic composition for skin protection against ultraviolet rays.
The method according to claim 1,
The cosmetic composition for protecting skin against ultraviolet rays, wherein the non-aroma extract is effective for inhibiting the production of active oxygen induced by ultraviolet ray B.
4. The method according to any one of claims 1 to 3,
The non-averaged extract is an extract extracted with water, an organic solvent or a mixed solvent thereof,
Wherein the organic solvent is at least one selected from the group consisting of alcohols having 1 to 5 carbon atoms.
4. The method according to any one of claims 1 to 3,
The cosmetic composition for protecting skin against ultraviolet rays, wherein the non-isostatic extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.

Images