KR102389374B1 - Cosmetic composition for preventing thermal skin aging comprising alpinia officinarum root extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing goryanggang extract as an active ingredient, and more particularly, by including goryanggang extract as an active ingredient, it effectively inhibits active oxygen production, apoptosis and collagen degradation induced by infrared rays. At the same time, it is characterized in that it effectively inhibits the secretion of histamine and NO (nitric oxide), which are vasodilators involved in skin temperature rise.

Description

COSMETIC COMPOSITION FOR PREVENTING THERMAL SKIN AGING COMPRISING ALPINIA OFFICINARUM ROOT EXTRACT}

The present invention relates to a cosmetic composition containing a goryanggang extract as an active ingredient, and more particularly, a vasodilator substance that effectively inhibits the generation of active oxygen, apoptosis, and collagen degradation induced by infrared rays and at the same time increases the skin temperature. It relates to a cosmetic composition for improving or inhibiting thermal aging of the skin, comprising as an active ingredient a goryanggang extract that effectively suppresses the secretion of phosphorus histamine and NO (nitric oxide).

The skin is largely divided into three layers: the epidermis, the dermis, and the subcutaneous fat tissue in order from the outside, and functions to protect the human body from physical and chemical stimuli from the external environment. Among the environmental factors that affect the skin's defense function, the most important is ultraviolet rays, and ultraviolet rays irradiated to the skin increase the formation of free radicals and the generation of reactive oxygen species (ROS) in the skin. It induces oxidative stress on cellular components such as , DNA, cell membranes and proteins, leading to skin aging and inflammatory lesions of the skin.

Among the environmental factors that affect the skin's defense function, recently, many studies have been reported that thermal aging, which is caused by an increase in skin temperature by external stimuli such as infrared rays, is an important factor causing skin aging. As the skin temperature increases, the production of MMP, a matrix proteolytic enzyme that decomposes collagen, increases, and the collagen present in the dermis of the skin is rapidly reduced, causing the skin to lose elasticity and wrinkles to increase. It refers to a skin aging phenomenon in which damage to skin cells is promoted as the production of active oxygen in skin cells is promoted. The human skin temperature is maintained at an average of 31~32℃, and as the skin temperature rises above that by external stimuli such as infrared rays, thermal aging is induced and skin aging is progressed.

Infrared radiation is an electromagnetic wave with a wavelength range of 780 nm to 1,000 nm. Among infrared rays, near-infrared rays can be transmitted deeply to the subcutaneous tissue layer of the skin. became In addition, when continuously exposed to an environment in which the skin temperature is increased by infrared rays, capillaries are expanded and the inflow of inflammatory substances is increased, resulting in skin inflammatory response and thermal aging.

A lot of research has already been done on photoaging by ultraviolet rays, and products using inorganic sunscreens TiO ₂ and ZnO ₂ and various organic sunscreens are sold in various forms, so photoaging by ultraviolet rays is effectively suppressed.

Recently, as interest and demand for heat aging due to skin temperature rise other than photoaging caused by ultraviolet light has increased, products that prevent or suppress skin aging caused by heat are being actively developed. Most of the products use functional materials to lower the skin temperature, and the effect is insignificant.

Therefore, there is an urgent need to develop excellent new materials capable of effectively improving or suppressing thermal aging induced by infrared rays.

Goryanggang ( Alpinia officinarum ) is a perennial herb belonging to the ginger family, and its rhizomes harvested in autumn and dried are used. Kaoryanggang contains cineol, cardinene, eugenol, kaemprid, quercetin, isoramnetin and galangol as main ingredients.

Accordingly, the present inventors have completed the present invention by confirming that the goryanggang extract can be usefully used as a cosmetic composition capable of improving or inhibiting skin thermal aging.

KR 10-199592 B1 KR 10-1822092 B1

It is an object of the present invention to provide a cosmetic composition having an infrared-induced active oxygen production inhibitory effect containing a goryanggang extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition having an infrared-induced collagen degradation inhibitory effect containing a goryanggang extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition having an inhibitory effect on the secretion of histamine and NO (nitric oxide), which are vasodilators, containing goryanggang extract as an active ingredient.

To achieve the above object, the cosmetic composition for improving or inhibiting skin heat aging containing the goryanggang extract of the present invention as an active ingredient is characterized in that it includes the goryanggang extract as an active ingredient.

The goryanggang extract is characterized in that it has an effect of inhibiting the generation of active oxygen in cells, apoptosis and collagen degradation induced by infrared rays.

The goryanggang extract is characterized in that it has an effect of inhibiting the secretion of histamine and NO (nitric oxide), which are vasodilation inducers.

The goryanggang extract is water, an organic solvent, or a mixed solvent extract thereof, and the organic solvent is at least one selected from the group consisting of alcohols having 1 to 5 carbon atoms.

The goryanggang extract is obtained by pulverizing goryanggang, adding an extraction solvent in an amount of 1 to 50 times the dry weight of the extract, extracting the active ingredient at 4 to 30° C. for 2 to 3 days, and then filtering it to remove the solid content. characterized in that it is manufactured.

The goryanggang extract is characterized in that it is included in an amount of 0.00001 to 10% by weight based on the total weight of the cosmetic composition.

According to the present invention, it effectively inhibits the generation of active oxygen, apoptosis, and collagen degradation induced by infrared rays and at the same time effectively inhibits the secretion of histamine and NO (nitric oxide), which are vasodilators involved in skin temperature increase, There is an advantage in that it can satisfy consumers' demand for cosmetic compositions derived from natural products that improve the disadvantages of aging-related products.

1 is a graph showing the results of testing the antioxidant efficacy of Experimental Example 1 according to the present invention.
Figure 2 is a graph showing the results of experiments on the inhibitory effect of active oxygen production in cells of Experimental Example 2 according to the present invention.
3 is a graph showing the results of testing the apoptosis inhibitory efficacy of Experimental Example 3 according to the present invention.
4 is a graph showing the results of testing the collagen degradation inhibitory efficacy of Experimental Example 4 according to the present invention.
5 is a graph showing the results of testing the histamine secretion inhibitory efficacy of Experimental Example 5 according to the present invention.
6 is a graph showing the results of testing the NO (nitric oxide) secretion inhibitory effect of Experimental Example 6 according to the present invention.
7 is a graph showing the results of testing the cytotoxicity of Experimental Example 7 according to the present invention.

Hereinafter, the present invention will be described in detail.

The cosmetic composition for improving or inhibiting skin thermal aging according to the present invention is characterized in that it contains goryanggang extract as an active ingredient. This goryanggang extract effectively inhibits the generation of active oxygen in cells, apoptosis and collagen degradation induced by infrared rays, and at the same time effectively inhibits the secretion of histamine and NO (nitric oxide), which are vasodilators involved in skin temperature rise. has a deterrent effect. In addition, the goryanggang extract does not irritate the skin, and the stability of the product after formulation is also excellent, so it is suitable as a cosmetic composition derived from natural products.

The goryanggang extract is preferably included in an amount of 0.00001 to 10% by weight based on the total weight of the cosmetic composition. If the content is too small, the effect of improving or inhibiting infrared induced thermal aging is insignificant, and if the content is higher, additional thermal aging This is because there are disadvantages such as an increase in manufacturing cost without showing an increase in improvement or inhibitory efficacy.

In addition, the goryanggang extract may be in a liquid state, but it is natural that one prepared in a powder state through an additional process such as concentration under reduced pressure, freeze-drying, or spray-drying after extraction may be used.

The manufacturing method of the goryanggang extract will be described in more detail as follows.

The goryanggang extract may be obtained by extracting and separating from nature using a method known in the art for extraction and separation.

In this case, the extraction is extracted from goryanggang river using an appropriate solvent, and may include all of a hot water extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract.

In addition, as an appropriate extraction solvent for extracting the extract from the goryanggang, any extraction solvent acceptable in the art may be used, but preferably at least one of water and an organic solvent having 1 to 5 carbon atoms may be used. there is. That is, any one of alcohols having 1 to 5 carbon atoms, including purified water, methanol, ethanol, propanol, isopropanol, and butanol, is used alone or in a mixture of two or more It is preferable, but it is not necessary to be limited thereto.

As the goryanggang extraction method, any one of the methods such as hot water extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, compression, etc. may be selected and used, but it is not necessary to be limited thereto. . That is, there is no limitation on the method for preparing the goryanggang extract of the present invention, and any known method may be used.

According to a specific embodiment of the present invention, the preparation of the goryanggang extract may be carried out as follows.

The goryanggang extract is obtained by pulverizing goryanggang, adding an extraction solvent in an amount of 1 to 50 times the dry weight of the extract, extracting the active ingredient at 4 to 30° C. for 2 to 3 days, and then filtering it to remove the solid content. , can be manufactured.

And after extraction, it may further include a step of concentration under reduced pressure, freeze-drying or spray-drying. At this time, since the concentration under reduced pressure, freeze-drying or spray-drying follows a method known in the field of extracts, a detailed description thereof will be omitted.

On the other hand, the cosmetic composition of the present invention may include, without limitation, conventionally acceptable ingredients in addition to the active ingredients, for example, diluents, excipients, buffers, dispersants, surfactants, colorants, flavorings or sweeteners, such as conventional adjuvants. can do.

In addition, the cosmetic composition may further add a functional cosmetic ingredient that imparts cosmetic functionality to the skin, a moisturizer, and other additives, and may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics. . Animal oil, vegetable oil, wax, paraffin, silicone, lactose, silica, talc, water, ethanol, benzyl alcohol, glycerol aliphatic ester, aliphatic alcohol sulfate, etc. may be used as the carrier component, but the type is not limited. is natural

In addition, the cosmetic composition according to the present invention is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and a spray group consisting of It may have any one formulation selected from, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through the following experimental examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

(Example 1): Preparation of goryanggang extract

After washing and drying the kaoliang steel, it was pulverized into 10 to 100 mesh using a pulverizer. Then, after taking 20 g of crushed goryanggang, 200 ml of 70% ethanol was added thereto, stirred at 25° C. for 48 hours to extract, filtered, concentrated under reduced pressure with a rotary concentrator, and freeze-dried to prepare a goryanggang extract.

(Experimental Example 1): Antioxidation test

In order to confirm the antioxidant efficacy of the goryanggang extract according to the present invention, the following experiment was conducted.

Specifically, a DPPH solution with a concentration of 0.32 mM was added to the solution for each concentration of the goryanggang extract obtained in Example 1, reacted for 20 minutes at room temperature in a light-shielded state, and then the absorbance at 517 nm was measured using ELISA. measured. And the result is shown in FIG.

As can be seen in FIG. 1 , as a result of treating the goryanggang extract of Example 1 according to the present invention, excellent DPPH radical scavenging activity was confirmed. From this, it was found that when the goryanggang extract was included as an active ingredient, the antioxidant effect was increased by about 60%.

(Experimental Example 2): Intracellular active oxygen production inhibition test

The following experiment was conducted to confirm the inhibitory efficacy of the goryanggang extract according to the present invention to inhibit the generation of active oxygen in cells induced by infrared irradiation.

Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, inoculate HaCaT cells in a 96-well black plate to 1×10 ⁴ per well, and after 24 hours, the medium was removed and DCFH-DA (2', 7'-Dichlorodihydrofluorescin diacetate) was pretreated for 30 minutes. DCFH-DA (2', 7'-Dichlorodihydrofluorescin diacetate) reacts with intracellular reactive oxygen species to produce a fluorescent substance (2', 7'-Dichlorodihydrofluorescein: DCF). Active oxygen can be measured. Next, the DCFH-DA reagent was removed and 50 μl of PBS buffer was treated in each well, and the goryanggang extract obtained in Example 1 was treated by concentration. And after irradiating infrared rays for 15 minutes using IR-A (PHILIPS lamp), PBS buffer was removed and the medium was treated by 100 μl per well, and then the goryanggang extract obtained in Example 1 was treated by concentration. And after 2 hours, fluorescence was measured at excitation 480nm/emission 530nm using a microplate fluorometer. Ascorbic acid was used as a control. And the result is shown in FIG.

As can be seen in FIG. 2 , as a result of the treatment of the goryanggang extract of Example 1 according to the present invention, it was confirmed that the infrared-induced intracellular active oxygen production was excellently inhibited. From this, it was found that when the goryanggang extract was included as an active ingredient, the generation of active oxygen in cells induced by infrared irradiation was reduced by about 66%.

(Experimental Example 3): Skin cell damage inhibition test

In order to confirm the effect of inhibiting skin cell damage induced by infrared irradiation of the goryanggang extract according to the present invention, the following experiment was conducted.

Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, inoculate HaCaT cells in a 96-well plate so that it becomes 1.0×10 ⁴ per well, and after 24 hours, the medium After removal, 50 μl of PBS buffer was treated in each well, and the goryanggang extract obtained in Example 1 was treated by concentration. And after irradiating infrared rays for 15 minutes using IR-A (PHILIPS lamp), PBS buffer was removed and the medium was treated by 100 μl per well, and the goryanggang extract obtained in Example 1 was treated by concentration. And 24 hours later, 100 μl of MTT reagent was treated in each well, and incubated at 37° C., CO ₂ for 4 hours in an incubator, and absorbance (Optical Density) was measured at 570 nm. Ascorbic acid was used as a control. And the result is shown in FIG.

As can be seen in FIG. 3 , as a result of treating the goryanggang extract of Example 1 according to the present invention, it was confirmed that the infrared ray-induced cell damage was effectively inhibited. From this, it was found that when the goryanggang extract was included as an active ingredient, the cell damage induced by infrared irradiation was reduced by about 13%.

(Experimental Example 4): Collagen degradation inhibition test

The following experiment was carried out to confirm the effect of inhibiting collagen degradation induced by infrared irradiation of the goryanggang extract according to the present invention.

Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, NIH3T3 cells were seeded in a 96-well plate to 5.0×10 ⁴ per well. When 24 hours have elapsed after inoculation, infrared rays are irradiated for 15 minutes using IR-A (PHILIPS lamp), washed with PBS buffer, and then changed to serum-free DMEM medium, and the goryanggang extract obtained in Example 1 is concentrated by concentration. The medium was treated. And 24 hours later, using Sircol soluble collagen assay kit (Biocolor, UK), the absorbance (Optical Density) was measured at 555 nm using ELISA for collagen content. Retinoic acid was used as a control. And the result is shown in FIG.

As can be seen in FIG. 4 , as a result of the treatment of the goryanggang extract of Example 1 according to the present invention, the efficacy of excellently inhibiting infrared-induced collagen degradation was confirmed. From this, it was confirmed that when the goryanggang extract was included as an active ingredient, the collagen content increased by about 120%.

(Experimental Example 5): Histamine secretion inhibition test

The following experiment was carried out to confirm the effect of the goryanggang extract according to the present invention to inhibit the secretion of histamine, which is a vasodilation inducer.

Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, RBL-2H3 cells were inoculated in a 96-well black plate to 5.0×10 ⁴ per well, and after 24 hours After removing the medium, the goryanggang extract obtained in Example 1 was treated in the medium for each concentration and cultured for 30 minutes. After changing to a medium containing 1 mg/ml of C48/80 and incubating for 20 minutes, 10 μl of 1N NaOH solution and 50 μl of 0.1% OPA solution were mixed in 50 μl of the medium, and reacted for 5 minutes at room temperature in the dark. The reaction was terminated by adding 10 μl of 1N HCl solution, and fluorescence was measured at excitation 360 nm/emission 450 nm using a microplate fluorometer. Cetirizine was used as a control group. And the result is shown in FIG.

As can be seen in FIG. 5 , as a result of the treatment of the goryanggang extract of Example 1 according to the present invention, it was possible to confirm an excellent histamine secretion inhibitory effect. From this, it was found that when the goryanggang extract was included as an active ingredient, the histamine production induced by C48/80 was reduced by about 26%.

(Experimental Example 6): NO (nitric oxide) secretion inhibition test

The following experiment was conducted to confirm the inhibitory efficacy of the goryanggang extract according to the present invention, which is a vasodilation inducer, on the secretion of NO (nitric oxide).

Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, inoculate Raw264.7 cells in a 96-well plate so that it becomes 2.0×10 ⁴ per well, and after 24 hours, the medium was removed and replaced with a medium containing 1ug/ml LPS (lipopolysaccharide), and the goryanggang extract obtained in Example 1 was treated by concentration. Then, after 24 hours of incubation, the same amount of Griss reagent was mixed with 50 μl of the medium and left for 20 minutes in the dark at room temperature, and then the absorbance (Optical Density) was measured at 540 nm. As a control, L-NMMA was used. And the result is shown in FIG.

As can be seen in FIG. 6 , as a result of the treatment with the goryanggang extract of Example 1 according to the present invention, excellent NO (nitric oxide) secretion inhibitory efficacy was confirmed. From this, it was found that when goryanggang extract was included as an active ingredient, LPS (lipopolysaccharide)-induced NO (nitric oxide) production was reduced by 49%.

(Experimental Example 7): Cytotoxicity test

In order to confirm whether the goryanggang extract according to the present invention has a direct effect on skin cell death, the following experiment was performed.

Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, HaCaT cells were inoculated in a 96-well plate to be 1×10 ⁴ per well, and after 24 hours passed, the Example The goryanggang extract obtained in step 1 was treated in the medium by concentration. And after 72 hours, 100 μl of MTT reagent was treated in each well and incubated for 4 hours at 37° C. in a CO2 incubator, and absorbance (Optical Density) was measured at 570 nm. And the result is shown in FIG.

As can be seen in FIG. 7 , as a result of treatment with the goryanggang extract of Example 1 according to the present invention, it was confirmed that there was no direct effect on apoptosis of skin cells up to a concentration of 100 ppm.

(Experimental Example 8): Raw material stability test

In order to confirm the raw material stability of the goryanggang extract according to the present invention, the following experiment was conducted.

Specifically, the goryanggang extract obtained in Example 1 was prepared as a solution with a concentration of 10,000 ppm, and under the conditions of light exposure, 25°C, 45°C, and cycle (0°C→25°C→45°C, each 8 hours), 3 After storage for one month, the properties, pH, specific gravity and microbial contamination were measured.

As a result, it was confirmed that the goryanggang extract of Example 1 was stable without agglomeration, precipitation, discoloration, odor, microbial contamination, pH change, and specific gravity change under all storage conditions for 3 months. Therefore, it was confirmed that the stability of the goryanggang extract according to the present invention was high.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

Contains goryanggang extract as an active ingredient,
The goryanggang extract is
A cosmetic composition for improving or inhibiting skin thermal aging, characterized in that it suppresses the generation of active oxygen in cells, cell damage and collagen degradation induced by infrared rays, and suppresses the secretion of histamine and NO (nitric oxide), which are vasodilation inducers .
delete delete According to claim 1,
The goryanggang extract is
water, an organic solvent, or a mixed solvent extract thereof,
The organic solvent is at least one selected from the group consisting of alcohols having 1 to 5 carbon atoms. A cosmetic composition for improving or inhibiting thermal aging of the skin.
5. The method of claim 4,
The goryanggang extract is
It is prepared by pulverizing high-strength steel, adding an extraction solvent in an amount of 1 to 50 times its dry weight, extracting the active ingredient at 4 to 30° C. for 2 to 3 days, and then filtering it to remove the solid content. A cosmetic composition for improving or inhibiting skin thermal aging.
6. The method of any one of claims 1, 4 and 5, wherein
The goryanggang extract is
A cosmetic composition for improving or inhibiting skin thermal aging, characterized in that it is included in an amount of 0.00001 to 10% by weight based on the total weight of the cosmetic composition.

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