KR20180128529A - 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링 - Google Patents

췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링 Download PDF

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KR20180128529A
KR20180128529A KR1020187034291A KR20187034291A KR20180128529A KR 20180128529 A KR20180128529 A KR 20180128529A KR 1020187034291 A KR1020187034291 A KR 1020187034291A KR 20187034291 A KR20187034291 A KR 20187034291A KR 20180128529 A KR20180128529 A KR 20180128529A
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cells
cell
differentiation
day
expression
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Korean (ko)
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벤자민 프라이어
다이나 라니아우스카스
마르샤 블랙무어
하이연 왕
코스타딘카 리로바
쉘리 넬슨
엘리자베스 로소차
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얀센 바이오테크 인코포레이티드
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KR1020187034291A 2013-11-01 2014-05-21 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링 Abandoned KR20180128529A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US61/962,158 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
US13/998,974 2013-12-30
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Related Parent Applications (1)

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KR1020167014409A Division KR20160079072A (ko) 2013-11-01 2014-05-21 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링

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KR20180128529A true KR20180128529A (ko) 2018-12-03

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Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020187034291A Abandoned KR20180128529A (ko) 2013-11-01 2014-05-21 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링
KR1020167014409A Ceased KR20160079072A (ko) 2013-11-01 2014-05-21 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링
KR1020167014406A Abandoned KR20160079071A (ko) 2013-11-01 2014-06-17 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링
KR1020187034483A Withdrawn KR20180130001A (ko) 2013-11-01 2014-06-17 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링

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Application Number Title Priority Date Filing Date
KR1020167014409A Ceased KR20160079072A (ko) 2013-11-01 2014-05-21 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링
KR1020167014406A Abandoned KR20160079071A (ko) 2013-11-01 2014-06-17 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링
KR1020187034483A Withdrawn KR20180130001A (ko) 2013-11-01 2014-06-17 췌장 내분비 세포로의 분화를 위한 인간 만능 줄기세포의 현탁 및 클러스터링

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EP (2) EP3063268A4 (https=)
JP (2) JP2016534731A (https=)
KR (4) KR20180128529A (https=)
CN (2) CN105793413A (https=)
AU (4) AU2014342995C1 (https=)
BR (1) BR112016009393A8 (https=)
CA (2) CA2928639A1 (https=)
MX (2) MX2016005657A (https=)
PH (2) PH12016500782A1 (https=)
RU (2) RU2689710C2 (https=)
SG (2) SG11201603045VA (https=)
WO (2) WO2015065524A2 (https=)

Families Citing this family (23)

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Publication number Priority date Publication date Assignee Title
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
JP7637323B2 (ja) * 2016-06-23 2025-02-28 ティソン バイオテック，インコーポレイテッド 副甲状腺ホルモン１型受容体を発現する細胞およびその使用
CA3042495A1 (en) 2016-11-10 2018-05-17 Viacyte, Inc. Pdx1 pancreatic endoderm cells in cell delivery devices and methods thereof
MX2019005476A (es) * 2016-11-16 2019-07-04 Cynata Therapeutics Ltd Ensayo de celulas madre pluripotentes.
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
CN111246864A (zh) * 2017-07-21 2020-06-05 森玛治疗公司 干细胞衍生的胰腺β细胞的重聚
JP7000896B2 (ja) * 2017-08-23 2022-01-19 王子ホールディングス株式会社 細胞シート形成部材、細胞シート形成部材の製造方法、および、細胞シートの製造方法
JP2021509812A (ja) * 2018-01-05 2021-04-08 プレートレット バイオジェネシス， インコーポレイテッド 巨核球を産生するための組成物および方法
CN112041428B (zh) * 2018-01-18 2024-05-24 新加坡科技研究局 用于在悬浮培养物中分化人多能干细胞系的方法
KR102115360B1 (ko) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 성체 줄기세포 배양액 및 그 제조방법
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
CN114173837A (zh) 2019-05-31 2022-03-11 W.L.戈尔及同仁股份有限公司 生物相容性膜复合材料
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
EP3975926A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
EP3976236A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. A biocompatible membrane composite
US20210062157A1 (en) * 2019-08-27 2021-03-04 United Therapeutics Corporation Methods and compositions for culturing alveolar cells
CA3150235A1 (en) 2019-09-05 2021-03-11 Alireza Rezania Universal donor cells
PH12022550542A1 (en) 2019-09-05 2023-03-20 Crispr Therapeutics Ag Universal donor cells
CN114729323B (zh) * 2019-11-22 2024-09-17 诺和诺德股份有限公司 旋转聚集的神经微球及其应用
WO2022144855A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells
JPWO2022203051A1 (https=) * 2021-03-25 2022-09-29
JP2022151855A (ja) * 2021-03-25 2022-10-07 株式会社カネカ 多能性幹細胞集団の製造方法
CN116218766A (zh) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 一种制备胰腺前体细胞的方法、培养基及其应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090115984A (ko) * 2001-11-09 2009-11-10 아르테셀 사이언스, 인크. 지방 조직에서 유래된 기질세포의 내분비 췌장 분화 및 이의 용도
DK3441459T3 (da) * 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
EP2088190A4 (en) * 2006-11-09 2011-01-05 Japan Government METHOD FOR THE CULTURE AND PASSING OF AN EMBRYONAL PRIMATIVE STEM CELL AND METHOD FOR INDUCING THE DIFFERENTIATION OF THE EMBRYONAL STEM CELL
US20090053182A1 (en) * 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
EP2559756B1 (en) * 2007-07-01 2019-12-18 Janssen Biotech, Inc. Single pluripotent stem cell culture
JP2011514169A (ja) * 2008-03-17 2011-05-06 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 幹細胞培養のためのマイクロキャリア
EP2942392B1 (en) * 2008-06-30 2018-10-03 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
WO2011019092A1 (en) * 2009-08-12 2011-02-17 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
JP5936134B2 (ja) * 2010-06-15 2016-06-15 国立大学法人京都大学 ヒト人工多能性幹細胞の選択方法
PL2853589T3 (pl) * 2010-08-31 2018-05-30 Janssen Biotech, Inc Różnicowanie ludzkich zarodkowych komórek macierzystych
MY177150A (en) * 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
US8951798B2 (en) * 2011-10-13 2015-02-10 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
FI2794857T3 (fi) * 2011-12-22 2026-03-25 Janssen Biotech Inc Ihmisen alkion kantasolujen erilaistaminen yksittäisiksi hormonaalisiksi insuliinipositiivisiksi soluiksi
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
BR112015015714A2 (pt) * 2012-12-31 2017-07-11 Janssen Biotech Inc suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas
WO2015065524A2 (en) * 2013-11-01 2015-05-07 Janssen Biotech, Inc. Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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