KR20180126912A - A composition for water tissue containing lava seawater - Google Patents

Abstract

The present invention relates to a composition for wet tissue containing lava seawater. The composition includes purified water, a moisturizing agent, a surfactant, a pH adjuster, citrus oil, and lava seawater salt. It is possible to improve the moisturizing power of the wet tissue.

Description

[0001] The present invention relates to a composition for lava seawater,

The present invention relates to a wet tissue composition containing lava seawater salt and Jeju bamboo sake extract.

Wet tissue is a kind of tissue paper. It refers to a damp tissue paper that contains moisture in the product during the manufacturing process. It is mainly used to wipe hands and face easily. In recent years, as the culture and the hygiene are emphasizing the convenience, the consumption of wipes is soaring and the supply of products is not so difficult. As a result, many small and medium sized companies are emerging.

As a prior art related to a wet tissue, Korean Patent Laid-Open Publication No. 10-2017-0033186 discloses a wet tissue prepared by impregnating a wet tissue with a composition for a wet tissue, wherein the wet tissue comprises: Moisturizer; Surfactants; pH adjusting agents; Wherein the antimicrobial agent is selected from the group consisting of a herbal extract, a moth leaf extract, a Ginkgo biloba extract, and a Sorghum extract. The humectant may be selected from the group consisting of pentylene glycol, 1,2-hexanediol, and caprylyl glycol. ≪ Desc / Clms Page number 2 >

As described above, in the prior art, the moisturizing power of the wet tissue is improved by the chemical composition. In the case of the chemical composition, There is a problem that can cause trouble or the like.

Korean Patent Publication No. 10-2017-0033186

SUMMARY OF THE INVENTION It is an object of the present invention to provide a wet tissue composition which can improve the moisturizing power of a wet tissue by using a natural composition such as an extract of Jeju Sauce.

The lava watersheet wet tissue composition of the present invention is characterized by containing purified water, a moisturizing agent, a surfactant, a pH adjusting agent, citrus oil, and lava seawater salt.

In addition, the wet tissue composition of the present invention is characterized by containing trehalose.

For example, the moisturizing agent may include one or more of a rape flower extract, an oilseed rape seed extract, and an islet extract.

As an example, the wet tissue composition of the present invention may comprise 0.1 to 0.2 parts by weight of a moisturizer, 0.04 to 0.06 parts by weight of trehalose, 0.05 to 0.07 parts by weight of a pH adjusting agent, 0.02 to 0.04 parts by weight of a surfactant, 0.01 to 0.01 part by weight of a citrus oil, 0.02 parts by weight, and 0.0005 to 0.002 parts by weight of lava salt seawater.

As used herein, the term "extract" refers to a substance which is extracted with water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, - Extracts obtained by leaching using dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixed solvent thereof, and extracts obtained using supercritical extraction solvents such as carbon dioxide and pentane Extract means the fraction obtained by fractionating the extract or the extract. The extraction method can be applied by any method such as cold rolling, refluxing, heating, ultrasonic irradiation, supercritical extraction, etc. in consideration of the polarity, extraction degree, . In the case of the fractionated extract, the fraction is suspended in a specific solvent and then mixed with a solvent having a different polarity. The extract is adsorbed on a column filled with silica gel or the like, and then a hydrophobic solvent, a hydrophilic solvent, Quot; means fractions obtained as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.

In addition to the above-mentioned composition, the wet tissue composition of the present invention may further comprise an ultraviolet protective component known in the art to enhance or add skin-related activity. For ultraviolet protection ingredients refer to Functional Cosmetics Revision according to the Cosmetics Act ("Standards and Testing Methods for Functional Cosmetics" in the Notification of the Ministry of Health and Welfare) and the Health Functional Foods Act ("Health Functional Food Standards and Specifications") These ingredients may be included in one or more of the compositions of the present invention.

The wet tissue composition of the present invention may be prepared according to a method of manufacturing a wet tissue composition which is customarily performed in the art, except that the above compositions are included.

As described above, according to the present invention, there is an advantage that an excellent moisturizing effect can be exhibited without causing side effects of skin by including Jeju Sori base extract and the like.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

The lava watersheet wet tissue composition of the present invention is characterized by containing purified water, a moisturizing agent, a surfactant, a pH adjusting agent, citrus oil, and lava seawater salt.

Purified water refers to pure water from which various minerals and organic matters have been completely removed, and can be produced by ion exchange or reverse osmosis. Here, various known technologies exist in the ion exchange method and the reverse osmosis method, and detailed description thereof will be omitted.

The moisturizing agent is added to and maintains moisture. In particular, in the present invention, the extract of Jeju Soseki is used as a natural composition. In addition, one or more of rape flower extract, rapeseed extract, and island scour extract may be further included.

The amount of the moisturizing agent is preferably 0.1 to 0.2 parts by weight based on 100 parts by weight of purified water. When the amount of the moisturizing agent is less than 0.1 parts by weight, moisture resistance is insufficient. When the amount is more than 0.2 parts by weight,

The pH adjusting agent is added to inhibit oxidation. Sodium citrate and citric acid may be used. The pH adjusting agent is not limited to the above. Can be used. The pH adjusting agent is preferably 0.05 to 0.07 part by weight based on 100 parts by weight of purified water. If the amount is less than 0.05 part by weight, the pH of the final product is not controlled smoothly. If the amount is more than 0.07 part by weight, As described above.

As the surfactant, polysorbate and the like can be used, but the surfactant is not limited thereto, and known surfactants may be used, except for those which are harmful to the human body and prohibited. The surfactant is preferably 0.02 to 0.04 part by weight based on 100 parts by weight of purified water. When the amount of the surfactant is less than 0.02 part by weight, the combination of purified water and other ingredients is not smooth. On the other hand, There is a problem that it is deteriorated as described above.

The citrus oil has a synergistic effect such as excellent moisturizing effect and atopic prevention effect when it comes into contact with human body. The citrus oil is preferably used in an amount of 0.01 to 0.02 part by weight based on 100 parts by weight of the purified water. When the amount is less than 0.01 part by weight, the amount of the citrus oil contributes to improving the moisture resistance of the final product. When the amount of the citrus oil is more than 0.02 parts by weight, And there is a possibility of causing skin troubles. Therefore, the above-mentioned content range is limited.

In addition to the above composition, the wet tissue composition of the present invention provides an example in which 0.04 to 0.06 part by weight of trehalose and 0.0005 to 0.002 part by weight of lava salt water are further added to 100 parts by weight of purified water.

The trehalose is a non-reducing saccharide having a positive structure and is one kind of saccharides present in nature in a broad range from microorganisms to plants and insects, by protecting the cell membrane and preventing moisture evaporation.

It has been found that trehalose is a saccharide that exhibits the function of inhibiting the degeneration of the three major nutrients in the body such as suppression of starch aging, inhibition of protein denaturation, and inhibition of lipid degradation. In addition, Trehalose is said to calm the skin and to create a smooth and transparent skin.

The sodium chloride constituting the lava salt water salt is physiologically indispensable to the animal and is contained in the body fluids to control the osmotic pressure of the body fluid. The sodium salt of the sodium chloride is combined with the phosphoric acid to maintain the acid base equilibrium of the body fluids as the buffer substance .

The lava salt seawater has a positive effect on the human body when it comes into contact with the skin, and it has been reported that it has better skin moisturizing power than urea, which is a natural moisturizing factor of the skin. Various skin troubles (skin soothing) It is also known to be effective for removal of wastes and exfoliation, acne, wrinkles and atopy.

< Manufacturing example > Jeju Sori  Manufacture of extracts, rape flower extracts and islet extracts

Preparation Example 1 Preparation of Jeju Sauce Extract

10% by weight of 70% ethanol was added to the sake bran powder and extracted at room temperature for 24 hours, followed by filtering with a filter paper. The filtrate was concentrated under reduced pressure and lyophilized to obtain a Jeju sake bran extract.

&Lt; Preparation Example 2 > Production of rape flower extract

10% by weight of 70% ethanol was added to the rape blossom powder, which was then extracted at room temperature for 24 hours and filtered through a filter paper. The filtrate was concentrated under reduced pressure and lyophilized to obtain a rape flower extract.

&Lt; Preparation Example 3 >

To the leaf powder of Acanthopanax koreanum, 10 times by weight of 70% ethanol was added and extracted at room temperature for 24 hours and then filtered with a filter paper. The filtrate was concentrated under reduced pressure and lyophilized to obtain an extract of aphids.

< Experimental Example > Moisture power  Confirm( Hyaluronic  acid (HA) measurement)

 HaCaT was dispensed at 2.5 × 10 4 cells / well on a 96-well plate and cultured in a 5% CO 2 cell incubator at 37 ° C for 24 h to allow the cells to adhere well to the plate. After 24 hours, the samples were treated by concentration and cultured and then measured by hyaluronic acid (R & D System, DY3614) ELISA kit.

The results were measured with an ELISA reader absorbance at 540 nm. The final amount of hyaluronic acid was determined by protein quantification of the cells. After cell culture and sample treatment, the medium was recovered and each well was washed with PBS. Cells were treated with 1N NaOH to dissolve the protein and quantitated by Lowry assay, one of the protein assay methods.

Bio-Rad's DC Protein Assay (500-0116) was used to quantify the protein. Reagent A: Reagent S = 50: 1, then treated with Reagent B and reacted at room temperature. The amount of protein was determined by measuring the absorbance at 750 nm in an ELISA reader. The amount of protein was determined using a standard curve of bovine serum albumin.

○ Hyaluronic acid (HA) production ability of extract Sample Concentration % Control 100.00 ± 2.81 Retinoic acid 206.30 ± 1.21 Lava seawater (hardness) 100 64.85 ± 1.07 500 70.10 ± 1.91 1000 84.70 +/- 1.39 Seok Changpo (ug / ml) 100 116.14 + - 2.31 10 103.91 + - 3.60 Gosaricin (ug / ml) 100 104.30 ± 1.51 10 95.34 + - 2.37 Aloe (ug / ml) 100 110.27 + - 4.76 10 91.36 ± 3.89 Perennial (ug / ml) 100 87.22 + - 0.54 10 75.05 ± 3.40 Rapeseed (ug / ml) 100 111.95 ± 3.29 10 92.77 ± 3.78 Jeju Bread Sauce (ug / ml) 100 216.74 ± 3.20 10 125.08 ± 1.96 Island peel (ug / ml) 100 97.78 ± 2.77 10 88.67 + 1.54 Root leeks (ug / ml) 100 92.72 + - 2.30 10 101.80 ± 3.54 White parasitism (camellia mistletoe) (ug / ml) 100 87.00 ± 2.89 10 72.30 ± 3.90 Pomegranate (ug / ml) 10 46.09 ± 2.19 One 59.19 + 1.31 Wooden leaves (leaves) (ug / ml) 10 93.88 ± 1.60 One 9.30 ± 1.97 Wildwood (twigs) (ug / ml) 10 6.83 ± 2.16 One 06.79 ± 3.79 Ground bed (ug / ml) 10 9.08 ± 2.63 One 7.38 ± 3.19

Hyaluronic acid is known to have a moisturizing effect on the skin, which can be used to confirm the degree of skin moisturization.

As shown in the above Table 1, the amount of each hyaluronic acid was expressed as% in comparison with the untreated group. As a result, the extracts of 9 kinds (such as Seokchonpo, Kosei, Aloe, , Hyaluronic acid production was increased by 216.74% (116.74% increase compared to control) in the extract of Jeju Sori extract compared to other extracts.

In the case of lava sea water, it was confirmed that hyaluronic acid was not produced. Four kinds of extracts (pomegranate, yellowtail, twig, and ground bed), which are highly toxic extracts, I could.

○ Hyaluronic acid (HA) production ability of high concentration extract Sample Concentration % Control 100.00 + - 2.64 Retinoic acid 214.31 + - 5.63 Seok Changpo (ug / ml) 400 98.99 + - 4.19 200 106.23 + - 5.94 Gosaricin (ug / ml) 400 86.58 8.07 200 80.42 + - 6.81 Aloe (ug / ml) 400 128.94 + - 3.68 200 98.08 + - 4.97 Perennial (ug / ml) 400 106.11 ± 7.12 200 81.44 + 3.66 Rapeseed (ug / ml) 400 207.33 + - 4.16 200 185.21 + - 0.83 Island peel (ug / ml) 400 135.02 + - 0.41 200 116.51 ± 3.28 Root leeks (ug / ml) 400 114.44 + - 2.63 200 89.71 + - 7.97 White parasitism (camellia mistletoe) (ug / ml) 400 98.23 + - 3.01 200 95.05 ± 3.23

To determine the hyaluronic acid production ability at high concentration, extracts without toxicity at 400 ug / ml were retested. As a result, as shown in Table 2, the highest yield of hyaluronic acid was 207.33% (relative to Control), 135.02% (control: 35.02%) of alopecum extract, 128.94% Compared with 28.94% in the same period).

As a result, it has been proved through experimentation that Jeju Sori extract, rape flower extract, and anthracnose extract have excellent efficacy in moisturizing.

< Example > Preparation of liquid composition

The liquid composition was prepared as shown in Table 3 above.

< Experimental Example  2> Human body experiment

Patch tests were performed on the human body to confirm that the liquid composition prepared according to Table 3 was safe for human application and had no skin irritation or other side effects. The test was carried out in a healthy adult male and female using a Finn chamber at the back with 40 ㎕ of the composition directly in the aluminum disk or on a paper disk and fixed on the skin with a scanpore tape After 24 hours, the patches were removed, and the results were judged after 5 hours. The results are expressed in terms of the degree of erythema and edema, and the criteria are given in Table 4 according to the criteria of the International Contact Dermatitis Research Group (ICDRG).

0 One 2 3 4 No flare Flare Fever and pus Fever, pus and
Small Severe edema and
Small

Based on the score determined according to the above criterion, the degree of stimulus was calculated according to the following equation, and a result of 0.01 was derived.

(%) = (Reactivity showing positive / reactivity of own population) × 100

It can be seen that the derived stimulus (0.01) corresponds to non-stimulation as shown in the evaluation table shown in Table 5 below.

Judgment No stimulation 0.0 to 0.75 Irritation 0.76 to 1.50 Light stimulus 1.51 to 2.50 Moderate irritation 2.51 to 4.00 River stimulation 4.01 or higher

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. Therefore, the technical scope of the present invention should not be limited to the contents described in the detailed description of the specification, but should be defined by the claims.

Claims (5)

Characterized in that the composition comprises purified water, a moisturizing agent, a surfactant, a pH adjuster, citrus oil, and lava seawater salt.
The method according to claim 1,
Disodium iodide; Trehalose; Characterized in that the composition comprises at least one of the following components:
The method according to claim 1,
Wherein the moisturizing agent comprises Jeju Soseki extract.
The method of claim 3,
Wherein the moisturizing agent comprises at least one of rape flower extract, rape seed extract, and an extract of an islet extract.
3. The method of claim 2,
0.1 to 0.2 parts by weight of a moisturizer, 0.04 to 0.06 parts by weight of trehalose, 0.05 to 0.07 parts by weight of a pH adjusting agent, 0.02 to 0.04 parts by weight of a surfactant, 0.01 to 0.02 parts by weight of citrus oil, 0.0005 to 0.002 parts by weight of citrus oil, By weight based on the total weight of the wastewater.