KR20180115169A - Cleansing composition comprising natural raw material extract and wet tissue comprising the same - Google Patents

Abstract

The present invention relates to a feminine cleanser composition comprising a natural raw material extract, a method for manufacturing the composition, and a feminine cleanser wet tissue containing the same. The feminine cleanser composition containing herbal extracts such as carbonated water, a motherwort extract, a mugwort extract, a green tea extract and the like of the present invention has a female skin calming and antimicrobial effect and can refresh the fatigue of mind and body by adding aroma oil and the like. The composition is expected to be widely used for feminine cleanser since the composition has excellent washing power and antimicrobial power and has no irritation and side effects.

Description

[0001] The present invention relates to a cleanser composition comprising a natural raw material extract and a wet tissue containing the natural cleansing composition,

The present invention relates to a female cleanser composition comprising a natural raw material extract and a female cleanser wet tissue containing the same.

Skin is a site that receives physical or chemical injury directly from various external factors, and has the ability to treat functional and structural damage.

Among them, the quality of women is the connection between the vulva and the uterus, which is the most humid and warmest place in the female body. Therefore, bacteria and fungi, which are the main causes of vaginitis, are suitable for habitat, so the diseases are likely to occur. Under normal conditions, many lactic acid bacteria live in the vagina, making the inside of the vagina mildly acidic and inhibiting the growth of pathogens and molds. However, it is difficult to prevent the growth of pathogens or fungi by using long-term antibiotics, long-term use of pills, use of tampons or loops or pregnancy or childbirth, When the balance inside the vagina breaks down due to hormonal balance changes, vaginitis may occur due to the proliferation of opportunistic pathogens that normally inhibit growth under normal conditions in the vagina. Vaginitis has been reported as bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis and Atrophic vaginitis. The most common disease is bacterial vaginosis, caused by Gardnerella vaginalis . Candida albicans , common with bacterial vaginosis, is caused by the fungus Candida albicans .

To prevent such female diseases, a cleaner or a vaginal cleanser or a vulva cleanser is often used. Local cleaning agents commonly used to prevent vaginitis include transparent or rosin-type detergents which are obtained by sulfonating fatty acids or distilled lauryl alcohol and synthesizing emollients, thickeners, nutrients, foaming agents and organic disinfectants Have been shipped. In addition, there are surfactants with detergency and povidone-iodine as a medicine as a main component. It is known that povidone-iodine, which is a chemical disinfectant by chemical synthesis, is effective for the prevention and treatment of candidiasis vaginitis, trichomoniasis vaginitis and other vaginal infections. However, it is known that povidone- , Contact dermatitis, etc., and it is known that iodine or total iodine in blood may be elevated in the vagina to cause hypothyroidism.

As a conventional technique for solving the side effects caused by the use of the disinfectant as a medicine as described above, the vaginal acidity is maintained at pH 3.2 by the secretion of lactic acid by beneficial microorganisms in the vagina in healthy women, On the basis of the fact that the harmful bacteria are inhibited or killed, a buffer solution having pH 3.5 with lactoserum extracted from milk and containing lactic acid as a main component has been proposed. However, , And it is difficult to expect fundamental treatment and prevention. In addition, as a general treatment of vaginitis, polyene-based drugs include nystatin and amphotericin B, and azole-based drugs include ketoconazole and miconazole There are imidazole drugs and triazole drugs such as itraconazole and fluconazole. When such an antibiotic is administered in an insufficient amount, the germs do not die, resulting in a greater difficulty in the subsequent treatment because of resistance. In addition, the chemical quality detergent used as a detergent has a limitation such that the detergent is deteriorated in long-term use, fungi are increased instead of removing germs, and the odor and unpleasant odor removal effect is hindered. Even if many methods as described above are developed, there is a problem that recurrence occurs well.

Although many drugs have been proposed as a method for treating vaginal cleansing and abnormal symptoms, there is still a lack of presentation of non-irritating drugs considering that the site of use is the most sensitive mucosal site for foreign substances. Thus, there is a desperate need to develop new technologies to meet the continuous need for drugs that have no irritation and side effects, such as compositions using natural extracts.

Korean Registered Patent No. 1218829 (December 28, 2012). Korean Patent Publication No. 2016-0015718 (Feb. Korean Registered Patent No. 1125758 (March 05, 2012).

In order to solve the above-mentioned problems, the present invention provides a female cleanser composition containing a natural herbal extract such as primrose hydrothermal water, motherwort extract, mugwort extract, and green tea extract, and having excellent washing power, moisturizing power and antibacterial activity.

In the present invention, the female cleaner composition is impregnated into a nonwoven fabric or a pulp substrate to provide a clean wipes for women.

According to an aspect of the present invention for solving the above problems, A mixture of motherwort extract, mugwort extract and green tea extract; And purified water.

In one embodiment of the present invention, the motherwort extract, the mugwort extract and the green tea extract may be solvent-extracted using ultra-high-pressure extraction using water or a mixed solvent of 1,2-hexanediol, glyceryl caprylate and ethylhexyl glycerin.

In one embodiment of the present invention, the female detergent composition may include 2 to 40 wt% of a primer spray, 0.01 to 3 wt% of a mixture of a motherwort extract, an mugwort extract and a green tea extract, and 45 to 95 wt% of purified water.

In one embodiment of the present invention, the motherwort extract, the mugwort extract, and the green tea extract may be mixed at a ratio of 1: 1 to 2: 0.5 to 1 by weight of the motherwort extract: mugwort extract: green tea extract.

In one aspect of the invention, the female detergent composition may further comprise one or more aroma oils selected from the group consisting of lavender, tea tree, geranium and bergamot.

Another embodiment of the present invention provides a female clean wipes wherein the female cleaner composition according to the present invention is impregnated into a nonwoven or pulp substrate.

The present invention provides a female cleanser composition comprising a natural herbal extract such as a primate, an extract of Momozo extract, a mugwort extract, a green tea extract, and purified water of a remaining amount and a wet tissue containing the same, It is expected to be able to be used widely as a cleaner for women because it has excellent washing power, moisturizing power and antibacterial power, and has no irritation and side effects.

Hereinafter, the present invention will be described in more detail with respect to a female cleanser composition containing a natural herbal extract such as moxibustion extract, moxa extract, green tea extract, and purified water, and a wet tissue including the same, according to the present invention. However, the following specific examples or examples are only a reference for explaining the present invention in detail, but the present invention is not limited thereto.

Unless otherwise defined, all technical and scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention is merely intended to effectively describe a specific embodiment and is not intended to limit the invention.

Also, the singular forms as used in the specification and the appended claims are intended to include the plural forms as well, unless the context clearly indicates otherwise.

The present invention relates to a primer composition; A mixture of motherwort extract, mugwort extract and green tea extract; And purified water.

That is, the present invention relates to a method for applying a mixture of a primate, a motherwort extract, a mugwort extract, and a green tea extract to a female cleanser composition mixed with a remaining amount of purified water, Is not only excellent in moisture and antibacterial activity, but also has excellent effects for use as a cleaner for women because it has little irritation to skin and allergic symptoms.

The inventors of the present invention have studied to develop a substance which shows excellent effects on the vaginal cleansing and antibacterial effect of women without containing a chemical substance such as a surfactant and the like. As a result, it has been found that a mixture of a primate, a motherwort extract, a mugwort It has been found that there is no need to use the separate chemicals as described above, and side effects such as skin irritation and the like due to the use of other chemicals are hardly generated. In addition, the present invention has been accomplished by confirming that it is possible to apply moisturizing, cleaning and antimicrobial effects to skin by using natural herbal extract and safely use on women's clean wipes which come into direct contact with the female locus.

It is one of the three largest mineral springs in the world, and is rich in minerals beneficial to human body, along with the Shasta mineral springs in the United States and the Napaurine mineral springs in England. It also contains simple carbonic acid and bicarbonate, is abundant in natural carbon dioxide, and is known to be effective for hypertension, diabetes, gastrointestinal diseases, skin diseases, and eye diseases. The present invention relates to a fountain spray gun located at Chojung-ri, North-side of Cheongwon-gun, Chungbuk province. The composition ratios are shown in Table 1 below.

Ca Mg P K Na Zn Fe Mn 28.6 7.39 0.1 1.71 18.9 0.01 0.01 0.002 Cu Pb Hg CN Cr CD Al . 0.005 Non-detection Non-detection Non-detection Non-detection Non-detection Non-detection .

In one embodiment of the present invention, the supernatant is preferably 2 to 40% by weight, more preferably 5 to 20% by weight, in consideration of the manufacturing process and production cost, while imparting a cooling sensation to the skin. It is possible to exhibit excellent washing, antibacterial and moisturizing effects in the above content range, to give a refreshing feeling to the skin and to further improve the cleaning effect.

In one embodiment of the present invention, the extraction method of the motherwort extract, the mugwort extract and the green tea extract is not particularly limited as long as it does not hinder the achievement of the object of the present invention. For example, It may be a solvent extraction using a mixed solvent of hexanediol, glyceryl caprylate, and ethylhexyl glycerin. When a mixed solvent is used, the cleaning and antibacterial effect is greatly improved as compared with the case of using each, and a moisturizing effect is exhibited can do.

In one embodiment of the present invention, the motherwort extract, the mugwort extract and the green tea extract may be prepared by ultra-high pressure extraction using water.

Ultra High Pressure Technology is a technology that can overcome stability problems such as discoloration and detachment of efficacy raw materials by conventional heat treatment by non-heat treatment extraction technology. It is based on Pascal's principle of using pressure of 100 ~ 1,000 MPa and transferring pressure instantaneously uniformly by using water or oil as pressure medium.

Ultra high pressure technology increases the stability of raw materials because no unwanted new chemical substances, byproducts, free radicals are generated, chemical stability does not occur, stability of active ingredient increases, and concentration of active ingredient .

In the present invention, it is preferable to use water as a pressure medium when extracting with the ultra-high pressure extraction method, and it may be extracted at a room temperature of 10 to 30 ° C using a pressure of 500 to 700 MPa, but is not limited thereto. In this range, the structure of the raw material is not destroyed, and the filter process is not added, which is effective.

In one embodiment of the present invention, the motherwort extract, the mugwort extract and the green tea extract may be subjected to solvent extraction using a mixed solvent of 1,2-hexanediol, glyceryl caprylate and ethylhexyl glycerin.

More specifically, extraction is carried out at a temperature of 15 to 30 占 폚 using a mixed solvent of 60 to 70% by weight of 1,2-hexanediol, 15 to 25% by weight of glyceryl caprylate and 5 to 15% by weight of ethylhexyl glycerin . With the composition of these mixed solvents, the properties (color, odor) and antimicrobial activity of the extract at the time of extraction are the most excellent in solubility with the active ingredient of the extract.

The term " extract " used in the present invention refers to an extract obtained by leaching or transferring from an extractive raw material, or by partially or completely concentrating the leached solution, or by drying the concentrate again, , A chemical substance itself contained in the raw material itself and exhibiting a main effect, and may further include a fraction obtained by further fractionating the solution obtained after the extraction.

More specifically, the motherwort extract may be an extract obtained by extracting using a motherwort leaf, the extract of mugwort may be an extract obtained by using a mugwort leaf, and the extract of green tea may be extracted by using a green tea leaf .

The above-mentioned Leonurus japonicus is a biennial plant of the dicotyledonous plant with a monocotyledonous plant. Motherwort is native to Korea, Japan, and China and grows to about 1.5 to 2 meters. The motherwort is an alkaloid, which is a plant base. It is an alkaloid which is a plant base and contains leonurine, leonuridine, stachyose, stachydrine, flavonoid, protocatechol, benzoic acid acid, and vitamin A, and is known to have pharmacological effects such as diuretic action, hemolytic action, stimulation of urination, decrease in uterine motion and relaxation, vasoconstriction, hypertension and anticancer effects.

The Artemisia asiatica Nakai is a perennial herbaceous perennial plant native to all parts of Korea, growing in sunny grasses and having a height of 60 to 120 cm. About 200 species are distributed in the northern hemisphere in the world, and about 30 species are distributed in Korea. Mugwort has been used for abdominal pain, vomiting, and hemostatic agent in herbal medicine for a long time. It has been used as an important medicinal agent for use in cold menstrual irregularity and uterine bleeding, lower abdominal pain, insect repellence and odor removal. Mugwort is widely used as a food with a unique flavor, taste and color. Essential ingredients of natural mugwort include essential oil components such as Chineon and Cescutelene, vitamins A, B ₁ , B ₂ and C and iron, potassium, calcium , And phosphorus. Especially, the essential oil component of mugwort shows antimicrobial activity against Staphyloccus aureus and fungi after 5 days of inoculation at 1,000 ppm concentration.

The green tea ( Camellia sinensis ; Green tea) is a tea made from unfermented tea leaves, and can be classified into green tea, black tea and oolong tea according to the fermentation process using the young leaves of Canellia spp. As a raw material . The pharmacological effect of green tea is to clear the head, to strengthen the liver, to reduce heat, to wash the waste in the body, to digestion, to relieve thirst, and the polyphenol compound, catechin, , Antibacterial effect, heavy metal removal effect, blood pressure lowering effect and so on.

The above-mentioned extracts of natural oriental herb have reduced skin irritation, have effects of moisturizing and cosmetic effects, and found that the effect is further improved when the motherwort extract, the mugwort extract and the green tea extract are mixed, Thus completing the present invention. In the present invention, it is advantageous that the mixture of the motherwort extract, the mugwort extract and the green tea extract in an amount of 0.01 to 3% by weight provides appropriate moisturizing and antibacterial effects without giving excessive skin irritation.

In one embodiment of the present invention, the mixture of motherwort extract, mugwort extract, and green tea extract may be a mixture of motherwort extract: mugwort extract: green tea extract at a weight ratio of 1: 1 to 2: 0.5 to 1, And a moisturizing effect. In one embodiment of the present invention, the motherwort extract, mugwort extract and green tea extract may be a solid obtained by filtering the solvent extract, followed by concentration under reduced pressure and drying under reduced pressure.

In one embodiment of the present invention, the mixture of motherwort extract, mugwort extract and green tea extract may be used in an amount of 0.01 to 3% by weight, more preferably 1 to 5% by weight, based on the total amount of the female detergent composition. Excellent antimicrobial and moisturizing effects can be exhibited without giving excessive skin irritation in the above content range.

In one aspect of the present invention, an essential oil component selected from the group consisting of lavender, tea tree, geranium, bergamot, peppermint oil and the like is added to the female detergent composition of the present invention in a trace amount , And aromatherapy to improve the physical and mental health, as well as antibacteria (antibacteria) and antivirus (antivirus) can be obtained additional effects such as.

Aromatherapy refers to aroma and therapy. Essential oils are extracted from plants, flowers, leaves, fruits, roots and stems by steam distillation method (organic solvent extraction method, compression method) It means extracted fragrance. It has volatility and has the property of being easily absorbed through respiratory and skin. Essential oils are composed of carbon, hydrogen and oxygen, and can be classified into compounds such as terpenoid hydrocarbons, alcohols, esters, aldehydes, ketones, lactones, phenols and ethers.

In one embodiment of the present invention, the purified water is not limited, but it is preferable to use purified water having passed through a six-stage water purification system because it has excellent wettable composition stability, and its content is 45 to 95% by weight, And more preferably 60 to 80% by weight. It exhibits excellent antibacterial and moisturizing effect as well as excellent feeling in the above content range.

Further, the female cleaner composition according to the present invention may further contain an alcohol, a humectant, a fragrance and the like within the range not hindering the object of the present invention.

Another embodiment of the present invention provides a female clean wipes wherein the female cleaner composition according to the present invention is impregnated into a nonwoven or pulp substrate.

In an embodiment of the present invention, the content of the female cleaner composition in the wet tissue is preferably in the range of 70 to 90% of the total moisture content, and thus is not limited thereto.

Generally, a wet tissue is produced by impregnating a substrate such as non-woven pulp or the like using cotton fiber or shredded fiber with a predetermined amount of a solution containing water, a surfactant, an alcohol, a humectant, a perfume, and an antiseptic.

The present invention can simultaneously perform a role of preservative in the composition impregnated into a wet tissue and a function of a humectant at the same time by containing a mixture of primrose powder, motherwort extract, mugwort extract and green tea extract.

Further, the wet tissue according to the present invention may further include a moisturizing agent, a fragrance, and the like within a range not hindering the object of the present invention.

The nonwoven fabric in the wet tissue of the present invention may be obtained by commercially available various nonwoven fabrics known in the art or may be prepared by various methods. The nonwoven fabric is not particularly limited, and for example, a dry nonwoven fabric or a wet nonwoven fabric such as a chemical bond, a thermal bond, an airlay, a spanless, a spunbond, a meltblown, a needle punching, a stitch bond, a clara, .

The female cleansing wet tissue of the present invention containing a superfine softening water has the effect of enhancing the texture of the wet tissue and enhancing the white sensation. Because of this effect, it is advantageous in terms of cost and effect because it does not require a substance to be added in order to enhance the color feeling and feel clean in the use of the wet tissue. In addition, it is not possible to know the definite cause by adding the superfine water to the wet tissue, but the skin contact feeling can be remarkably improved.

A wet tissue is a product that is repeatedly opened and closed without being completely sealed after opening. Thus, it is difficult to keep the refreshing feeling of the primer and the softening agent constantly. Thus, the plant extract containing the spirantol or spananthol, which is a substance capable of giving a refreshing feeling to the female cleansing wipes of the present invention, % Can be added to improve the cooling sensation without giving excessive stimulation.

In addition, the female clean wipes according to the present invention may further include a microbicide disinfectant. The microbial disinfectant may be defined as an aqueous solution or an aqueous dispersion containing a substance having a function of inhibiting or preventing microbial propagation and growth. The microbial sterilization product may be a silver antimicrobial agent or a natural antimicrobial agent though not particularly limited, and natural antimicrobial agents include wasabi extract and catechin. In order to exhibit a proper sterilizing power, it is preferable that the antimicrobial disinfectant contains 0.0001 to 0.002% by weight of a silver antimicrobial agent or a natural antimicrobial agent.

Hereinafter, the content of the present invention will be described in more detail with reference to Examples. It is to be understood that the embodiments are illustrative of the present invention in more detail, and the scope of the present invention is not limited thereto.

1) Antimicrobial activity test

The female clean compositions prepared in the examples were evaluated according to the CTFA (Cosmetic, Toiletry and Fragrance Association) test guidelines.

Three selected strains were selected from CTFA and purchased from the Microbial Resource Center (KCTC). Staphylococcus aureus KCTC1916, Escherichia coli KCTC 1039 and Candida albicans KCTC 7965 were used as the test strains.

Staphylococcus aureus and E. coli were inoculated into TSB (Tryptic Soy Broth; Sigma-Aldrich), respectively, and cultured at 37 ° C for 24 hours. The cells were diluted with 0.9% sodium chloride aqueous solution to 1 10 ⁶ cfu / (Sigma-Aldrich) and incubated at 25 ° C. for 48 hours with shaking. The cells were diluted with 0.9% sodium chloride aqueous solution to 1 × 10 ⁵ cfu / mL. Each of 50 g of each of the composition samples was inoculated with a strain, and 1 mL of each strain was inoculated within 30 minutes immediately after inoculation. The suspension was diluted twice with 9 mL of 0.9% aqueous sodium chloride solution for 24 hours at 37 DEG C for E. coli and E. coli at 25 DEG C for Candida For 48 hours, and then the viable cell count is measured. Thereafter, the viable cell count was measured every day according to the above-mentioned method, and the number of days in which no bacteria were measured was confirmed.

2) Cytotoxicity test

Cytotoxicity testing by indirect contact of wet tissue was conducted according to the ISO 10993-5: 2009 (E) method, commissioned by the professional organization iuvo BioScience. L929 cells (Korean Cell Line Bank), a kind of mouse fibroblast, were cultured in MEM (Minimal Essential Media; ThermoFisher Scientific) at 37 ° C and 5% CO ₂ for 24 hours at 1 × 10 ⁶ cells / vital solution and removed after 15 min. Latex rubber (Yuhan-Kimberly) was used as a negative control and USP (USP) high-density polyethylene standard was used as a positive control. The surface of L929 cells was covered with each sample, followed by incubation at 37 ° C and 5% CO ₂ for 24 hours. Cell degeneration and anomalies were observed under a microscope, and the degree of cytotoxicity was graded as shown in Table 2.

Rating Reactivity Reaction Zone Description 0 none No detection zone under specimen area or below One Minimal Some malformed or degenerated cells were detected under specimen 2 slightly Degenerative or degenerative cells were detected in the restricted area below the specimen 3 usually The detection area has a maximum size of 1.0 cm 4 seriousness Detect over 1.0 cm beyond the specimen

3) Evaluation of sensation of use such as washing power (sensory evaluation)

A total of 200 female consumers in their 20s and 50s were randomly selected as experimental groups, and their satisfaction with the use of wipes was evaluated in the local environment under the same conditions and conditions through questionnaires. Satisfaction with use was evaluated as very satisfied, satisfied, normal, dissatisfied, and very dissatisfied. Among them, satisfaction and satisfaction were added together and evaluated as a percentage.

[Example 1]

1) Preparation of mixture of motherwort extract, mugwort extract and green tea extract

(TFS-10) at 25 ° C for 20 hours at 25 ° C using water as a pressure medium, and then concentrated under reduced pressure at 47 ° C at 800 hPa, and dried under reduced pressure To produce motherwort extract.

1,000 g of the mugwort leaf was filtered using an ultra pressure extractor (TFS-10) at 25 ° C for 20 hours using water as a pressure medium at 600 MPa for 20 hours. The filtrate was concentrated under reduced pressure at 478 ° C at 800 hPa, Mugwort extract was prepared.

1,000 g of green tea leaves were filtered using an ultra pressure extractor (TFS-10) at 25 ° C. for 20 hours using water as a pressure medium at 600 MPa for 20 hours. The filtrate was concentrated under reduced pressure at 47 ° C. at 800 hPa, The green tea extract was prepared.

The motherwort extract, mugwort extract and green tea leaf extract were mixed at a weight ratio of 30:50:20 to prepare 1.5 g of a mixture.

2) Manufacture of female cleaner composition

100 g of a female cleaner composition was prepared by mixing 88.5% by weight of purified water, 10% by weight of superfine water, and 1.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract prepared in 1) above.

3) Manufacture of clean wipes for women

The fabric (spun lace, weight 70 g / m ² ) was cut into a size of 200 mm ㅧ 150 mm, and then 10 pieces were put into a pack, and the female cleanser composition was added to prepare a female cleansing wet tissue having a total moisture content of 80%.

The properties of the fabricated female cleaner composition and the clean wipes were measured and shown in Tables 3 to 5 below.

[Example 2]

In the same manner as in Example 1 except that the motherwort extract, the mugwort extract and the green tea leaf extract were mixed at a weight ratio of 40:40:20 in preparing the mixture of motherwort extract, mugwort extract and green tea extract in Example 1, ≪ / RTI >

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 3]

In the same manner as in Example 1 except that the mixture of the motherwort extract, the mugwort extract and the green tea leaf extract was mixed at a weight ratio of 25:50:25 in the preparation of the mixture of the motherwort extract, the mugwort extract and the green tea extract in Example 1, ≪ / RTI >

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 4]

In the same manner as in Example 1 except that the mixture of the motherwort extract, the mugwort extract and the green tea leaf extract was mixed at a weight ratio of 1: 1: 1 in the preparation of the mixture of the motherwort extract, the mugwort extract and the green tea extract in Example 1, ≪ / RTI >

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 5]

In Example 1, the same procedure as in Example 1 was conducted except that 0.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 6]

In Example 1, 2.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used in the same manner as in Example 1.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 7]

In Example 1, 3% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used in the same manner as in Example 1.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 8]

1) Preparation of mixture of motherwort extract, mugwort extract and green tea extract

1,000 g of the motherchose leaf was placed in 10 L of a mixed solvent of 68% by weight of 1,2-hexanediol, 22% by weight of glyceryl caprylate and 10% by weight of ethylhexyl glycerin and extracted with stirring in a shaking incubator at 25 캜 for 24 hours , Filtered through a filter paper, concentrated under reduced pressure at 800 ° C at 47 ° C, and dried under reduced pressure to prepare a motherwort extract.

1,000 g of the mugwort leaf was placed in 10 L of a mixed solvent of 68% by weight of 1,2-hexanediol, 22% by weight of glyceryl caprylate and 10% by weight of ethylhexyl glycerin and extracted with stirring in a shaking incubator at 25 캜 for 24 hours , Filtered through filter paper, concentrated under reduced pressure at 800 P Pa at 47 째 C, and dried under reduced pressure to prepare Mugwort extract.

1,000 g of green tea leaves were added to 10 L of a mixed solvent of 68% by weight of 1,2-hexanediol, 22% by weight of glyceryl caprylate and 10% by weight of ethylhexyl glycerin and extracted with stirring in a shaking incubator at 25 캜 for 24 hours , Filtered through a filter paper, concentrated under reduced pressure at 800 ° C at 47 ° C, and dried under reduced pressure to prepare a green tea extract.

The motherwort extract, mugwort extract and green tea leaf extract were mixed at a weight ratio of 30:50:20 to prepare 1.5 g of a mixture.

2) Manufacture of female cleaner composition

100 g of a female cleaner composition was prepared by mixing 88.5% by weight of purified water, 10% by weight of superfine water, and 1.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract prepared in 1) above.

3) Manufacture of clean wipes for women

The fabric (spun lace, weight 70 g / m ² ) was cut into a size of 200 mm ㅧ 150 mm, and then 10 pieces were put into a pack, and the female cleanser composition was added to prepare a female cleansing wet tissue having a total moisture content of 80%.

The properties of the fabricated female cleaner composition and the clean wipes were measured and shown in Tables 3 to 5 below.

[Example 9]

In Example 8, the same procedure as in Example 8 was carried out except that the motherwort extract, the mugwort extract and the green tea leaf extract were mixed at a weight ratio of 40:40:20 in preparing the mixture of motherwort extract, mugwort extract and green tea extract, ≪ / RTI >

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 10]

Example 8 is the same as Example 8 except that the mixture of the motherwort extract, the mugwort extract, and the green tea leaf extract was mixed at a weight ratio of 25:50:25 in preparing the mixture of motherwort extract, mugwort extract and green tea extract in Example 8 ≪ / RTI >

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 11]

Example 8 is the same as Example 8 except that the mixture of the motherwort extract, the mugwort extract and the green tea leaf extract was mixed at a weight ratio of 1: 1: 1 in preparing the mixture of the motherwort extract, the mugwort extract and the green tea extract in Example 8 ≪ / RTI >

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 12]

Example 8 was prepared in the same manner as in Example 8 except that 0.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 13]

Example 8 was prepared in the same manner as in Example 8, except that 2.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 14]

Example 8 was prepared in the same manner as in Example 8, except that 3% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 15]

The procedure of Example 1 was repeated except that 0.1 g of lavender was further added to the female cleaner composition prepared in Example 1 above.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Example 16]

The procedure of Example 8 was repeated except that 0.1 g of lavender was further added to the female cleaner composition prepared in Example 8 above.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 1]

Example 1 was prepared in the same manner as in Example 1 except that the motherwort extract alone was used in place of the motherwort extract, the mugwort extract and the green tea extract in the same amount as the mixture.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 2]

The same procedure as in Example 1 was carried out except that the mugwort extract was used in the same manner as in Example 1 except that the mugwort extract was used alone in the same amount as the mixture of the motherwort extract, the mugwort extract and the green tea extract.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 3]

The green tea extract was prepared in the same manner as in Example 1, except that the green tea extract was used in the same manner as in Example 1, except that the green tea extract was used alone in the same amount as the mixture of the motherwort extract, mugwort extract and green tea extract.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 4]

In Example 1, a 1: 1 weight ratio mixture of motherwort extract and mugwort extract instead of the motherwort extract, mugwort extract and green tea extract was used in the same amount as the mixture of the motherwort extract, mugwort extract and green tea extract. Was prepared in the same manner as in Example 1.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 5]

In Example 1, a 1: 1 weight ratio mixture of motherwort extract and green tea extract instead of the motherwort extract, mugwort extract and green tea extract was used in the same amount as the mixture of the motherwort extract, mugwort extract and green tea extract. Was prepared in the same manner as in Example 1.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 6]

In Example 1, except that the mixture of mugwort extract and green tea extract in a 1: 1 weight ratio mixture was used in the same amount as the mixture of motherwort extract, mugwort extract and green tea extract instead of mixture of motherwort extract, mugwort extract and green tea extract Was prepared in the same manner as in Example 1.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 7]

In Example 1, 3.5% by weight of a mixture of motherwort extract, mugwort extract and green tea extract was used in the same manner as in Example 1.

The properties of the fabricated female cleaner composition and the wet tissue were measured and shown in Tables 3 to 5 below.

[Comparative Example 8]

In Example 1, the same procedure as in Example 1 was carried out except that no superfine water was used, and further purified water was used as a superfine water softener content.

The properties of the fabricated female cleaner composition and the wet tissue were tested by the methods described above and are shown in Tables 3 to 5 below.

Antimicrobial activity test Unit: days sample Staphylococcus aureus Escherichia coli Candida Example 1 5 3 14 Example 2 4 2 13 Example 3 5 4 14 Example 4 6 5 14 Example 5 4 4 13 Example 6 4 2 12 Example 7 6 4 15 Example 8 6 3 15 Example 9 5 3 14 Example 10 6 4 15 Example 11 7 5 14 Example 12 6 5 13 Example 13 5 3 13 Example 14 7 5 15 Example 15 5 4 15 Example 16 5 3 14 Comparative Example 1 11 8 25 Comparative Example 2 10 10 24 Comparative Example 3 12 9 25 Comparative Example 4 9 7 21 Comparative Example 5 8 7 20 Comparative Example 6 9 6 21 Comparative Example 7 3 2 11 Comparative Example 8 11 9 25

As shown in Table 3, it was found that the antibacterial activity was excellent in Examples 1 to 16, in which the composition of the present invention was used, and when one of the natural herbal extracts was excluded, the antibacterial activity was significantly lowered as in Comparative Examples 1 to 6 And it was found that the extinction period was delayed. As shown in Comparative Example 8, when the supernatant was not added, it was found that the antimicrobial activity was remarkably decreased and the extinction time was delayed.

Cytotoxicity test sample Cytotoxicity rating Example 1 0 Example 2 0 Example 3 0 Example 4 0 Example 5 0 Example 6 0 Example 7 0 Example 8 0 Example 9 0 Example 10 0 Example 11 0 Example 12 0 Example 13 0 Example 14 0 Example 15 0 Example 16 0 Comparative Example 1 One Comparative Example 2 One Comparative Example 3 One Comparative Example 4 One Comparative Example 5 One Comparative Example 6 One Comparative Example 7 2 Comparative Example 8 One

As shown in Table 4, it was found that the composition of the present invention was safe for use in humans since the composition was not cytotoxic. In particular, when the content of the mixture of motherwort, mugwort, and green tea extract was 2% Of the total amount of the composition. In the case of Comparative Example 7 in which the content of the mixture of motherwort extract, mugwort extract and green tea extract was more than 3% by weight, when the results of Table 3 were compared with those of Table 3, the antimicrobial activity was superior but the cytotoxicity was greatly increased It was found to be unsuitable for use as a cleaner for women. On the other hand, when one component of the natural herbal extract was excluded or not, the cytotoxicity was observed in all experimental groups as in Comparative Examples 1 to 6 and Comparative Example 8, and in some cases, malformed or degenerated cells And the degree of detection is influenced.

Feeling satisfaction such as washing power sample satisfaction (%) Example 1 95 Example 2 94 Example 3 92 Example 4 93 Example 5 95 Example 6 96 Example 7 94 Example 8 93 Example 9 91 Example 10 91 Example 11 90 Example 12 90 Example 13 92 Example 14 90 Example 15 99 Example 16 98 Comparative Example 1 65 Comparative Example 2 61 Comparative Example 3 62 Comparative Example 4 71 Comparative Example 5 73 Comparative Example 6 72 Comparative Example 7 50 Comparative Example 8 60

As shown in Table 7, it was found that the wet tissues using the composition of the present invention had excellent overall satisfaction such as detergency and feelings of use. Particularly, when aromatherapy was applied together with lavender, satisfaction was the highest, 1 to 8, it has been found that satisfactory results such as detergency, irritation and the like are significantly lowered when they are outside the scope of the present invention.

From the above, it has been confirmed from the above that the present invention is completed when the female cleaner composition of the present invention and the wet tissue including the same are excellent in cleaning power and feeling in use for women's cleansing, and have excellent antibacterial power as compared with conventional wet tissues .

While the invention has been shown and described with respect to the specific embodiments, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It is obvious that it can be modified and changed.

Claims (6)

Superfine; A mixture of motherwort extract, mugwort extract and green tea extract; And purified water; ≪ / RTI > The method according to claim 1,
Wherein the motherwort extract, the mugwort extract and the green tea extract are solvent-extracted by ultra-high pressure extraction using water or a mixed solvent of 1,2-hexanediol, glyceryl caprylate and ethylhexyl glycerin. The method according to claim 1,
Wherein the composition comprises from 2 to 40% by weight of a primer spray, from 0.01 to 3% by weight of a mixture of a motherwort extract, an mugwort extract and a green tea extract, and from 45 to 95% by weight of purified water. The method of claim 3,
The mixture of motherwort extract, mugwort extract and green tea extract is a female detergent composition wherein the weight ratio of motherwort extract: mugwort extract: green tea extract is 1: 1 ~ 2: 0.5 ~ 1. The method according to claim 1,
Wherein the composition further comprises one or more aroma oils selected from the group consisting of lavender, tea tree, geranium, bergamot, and peppermint oil. A female cleansing wet tissue in which the female cleansing composition of any one of claims 1 to 5 is impregnated in a nonwoven or pulp substrate.