KR20180105670A - 벡터-프리 유도만능 줄기세포를 생성시키기 위한 방법 및 벡터 - Google Patents

벡터-프리 유도만능 줄기세포를 생성시키기 위한 방법 및 벡터 Download PDF

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KR20180105670A
KR20180105670A KR1020187023128A KR20187023128A KR20180105670A KR 20180105670 A KR20180105670 A KR 20180105670A KR 1020187023128 A KR1020187023128 A KR 1020187023128A KR 20187023128 A KR20187023128 A KR 20187023128A KR 20180105670 A KR20180105670 A KR 20180105670A
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cells
ebna
reprogramming
vector
population
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아이탄 아브라함
토마스 페인
로버트 제이. 영
인바. 프리드리히 벤 눈
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론자 워커스빌 아이엔씨.
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KR1020187023128A 2016-01-12 2017-01-12 벡터-프리 유도만능 줄기세포를 생성시키기 위한 방법 및 벡터 Withdrawn KR20180105670A (ko)

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US201662277784P 2016-01-12 2016-01-12
US62/277,784 2016-01-12
PCT/US2017/013229 WO2017123789A1 (en) 2016-01-12 2017-01-12 Methods and vectors to produce vector free induced pluripotent stem cells

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KR (1) KR20180105670A (enExample)
CN (1) CN108778299A (enExample)
CA (1) CA3010764A1 (enExample)
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3010764A1 (en) * 2016-01-12 2017-07-20 Lonza Walkersville, Inc. Methods and vectors to produce vector free induced pluripotent stem cells
CA3078734A1 (en) * 2017-10-11 2019-04-18 Fate Therapeutics, Inc. Cellular reprogramming using temporal and transient plasmid vector expression system
CN108373998B (zh) * 2018-02-10 2019-08-27 安徽中盛溯源生物科技有限公司 一种通过蝾螈Oct4将人类血液细胞重编程为iPSC的方法
GB201805683D0 (en) * 2018-04-05 2018-05-23 Touchlight Ip Ltd Reprogramming vectors
JPWO2019244895A1 (ja) * 2018-06-18 2021-06-24 パール工業株式会社 形質転換細胞の製造方法
CN109679994B (zh) * 2018-12-13 2021-02-02 湖北汇智铭传生物科技股份有限公司 通过四环素诱导表达外源基因的游离载体及其构建方法
CN116348592A (zh) * 2020-10-02 2023-06-27 菲特治疗公司 诱导性多能干细胞的改善的重编程、维持和保存
CN114277190B (zh) * 2021-12-31 2024-07-23 安徽中盛溯源生物科技有限公司 一种hiPSC中外源基因残留检测用特异性DNA片段、引物、试剂盒和检测方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010537626A (ja) * 2007-09-04 2010-12-09 クイーンズランド ユニバーシティ オブ テクノロジー フィーダ細胞を含まない培地および培養系
EP3447128A1 (en) * 2008-06-04 2019-02-27 FUJIFILM Cellular Dynamics, Inc. Methods for the production of ips cells using non-viral approach
SG173469A1 (en) * 2009-02-20 2011-09-29 Ventria Bioscience Cell culture media containing combinations of proteins
CN103087991B (zh) * 2009-06-05 2018-06-12 富士胶片细胞动力公司 重编程t细胞和造血细胞的方法
ES2539487T3 (es) * 2009-11-04 2015-07-01 Cellular Dynamics International, Inc. Reprogramación episómica con compuestos químicos
WO2011159797A2 (en) * 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. A compendium of ready-built stem cell models for interrogation of biological response
US8765470B2 (en) * 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
JP2014520551A (ja) * 2011-07-11 2014-08-25 セルラー ダイナミクス インターナショナル, インコーポレイテッド 細胞のリプログラミング方法およびゲノムの改変方法
WO2013177228A1 (en) * 2012-05-22 2013-11-28 Loma Linda University Generation of integration/transgene-free stem cells
EP2853592B1 (en) * 2012-05-23 2019-02-20 Kyoto University Highly efficient method for establishing artificial pluripotent stem cell
WO2015006725A2 (en) * 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
SG10201807292YA (en) * 2014-03-04 2018-09-27 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
US20160362705A1 (en) * 2015-06-12 2016-12-15 Lonza Walkersville, Inc. Methods for Nuclear Reprogramming Using Synthetic Transcription Factors
AU2016318774B2 (en) * 2015-09-08 2022-08-18 FUJIFILM Cellular Dynamics, Inc. MACS-based purification of stem cell-derived retinal pigment epithelium
US10865381B2 (en) * 2015-10-20 2020-12-15 FUJIFILM Cellular Dynamics, Inc. Multi-lineage hematopoietic precursor cell production by genetic programming
CA3010764A1 (en) * 2016-01-12 2017-07-20 Lonza Walkersville, Inc. Methods and vectors to produce vector free induced pluripotent stem cells
CA3078734A1 (en) * 2017-10-11 2019-04-18 Fate Therapeutics, Inc. Cellular reprogramming using temporal and transient plasmid vector expression system
CN108410823B (zh) * 2018-03-26 2019-11-01 安徽中盛溯源生物科技有限公司 一种微环游离型载体高效重编程血液细胞生成iPSC的方法

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EP3402496A1 (en) 2018-11-21
US20170226483A1 (en) 2017-08-10
JP2019500910A (ja) 2019-01-17
CN108778299A (zh) 2018-11-09
WO2017123789A1 (en) 2017-07-20
EP3402496A4 (en) 2019-06-19
IL260452A (en) 2019-02-28
CA3010764A1 (en) 2017-07-20

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