KR20180100839A - A composition comprising heat water extract of gojiberry, fig, and agastache rugosa as active ingredients - Google Patents

Abstract

The present invention relates to a composition comprising heat water extracts of Lycium Barbarum, Ficus carica and Agastache rugosa, and it was confirmed that cytotoxicity was low while exhibiting a synergistic effect on antioxidant activity, immunity enhancement, whitening, wrinkle reduction, inhibition of collagenase and collagen production promoting activity, thereby being suitable for antioxidation, immunity enhancement, whitening, skin wrinkle reduction.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a composition comprising an extract of hot pepper, a fig and a hot water extract of a persimmon as an active ingredient,

The present invention relates to a composition for antioxidant, immunity enhancement, whitening, or wrinkle improvement comprising hot water extract of goji berries, figs and syrup as an active ingredient.

Due to recent advances in science and technology and modern medicine, the average life expectancy of humans is gradually increasing, and interest in health is increasing to improve quality of life. This tendency is increasing regardless of generation and age. In addition, there is a growing interest in medicines, health supplements, and cosmetics containing natural products, and the research and development thereof is increasing. In particular, the functions of health supplement foods and cosmetics, including the efficacy of natural drug products and natural products, are expanding to scientific scopes such as antioxidants, cholesterol suppression, prevention of obesity, prevention of immune and disease, and prevention of aging.

Gojiberry, also known as Chinese boxthorn or Matrimony Vine, represents a variety of species belonging to the family Solanacea and the Lycium genus. Among the various species of the genus Lycium, Lycium Barbarum and Lycium chinense are most commonly used in oriental medicine. For example, Lycium, also known as Fructus lycii Barbarum fruit has long been known as a medicinal plant that helps maintain health in general, especially in Asia. In addition, recent studies report that Lycium barbarum not only enhances and controls the immune system, but also has aging and anti-cancer properties, decreased blood fat and sugar levels, and arterial blood pressure reduction. United States Patent Application Publication No. 2006/0198810 discloses the efficacy of a mixture of rosemary extract, Chinese angelica extract and wolfberry extract, which promotes lipid synthesis of skin cells. Also, Lycium The characteristics of certain components of barbarum are already known. According to this, zeaxanthin dipalmitate is known to be antioxidant and anti-inflammatory, while Lycium barbarum Polysaccharides (LBP) and 2-O- (β-D-glucopyranosyl) -ascorbic acid (analogs of vitamin C) are known for their immunostimulatory and antioxidant properties.

The fig tree (Ficus carica L) is a deciduous shrub belonging to the genus Phalaenopsis, originating in the western part of Asia. It is 2 ~ 4m in height. The bark is grayish white, the leaves are alternate, broad ovate, thick, surface rough, and hairs on the back. Latex is athlete's foot, boils, hemorrhoids, leaves athlete's foot or neuralgia, fruit is known to be effective in blood pressure lowering, constipation. Korean Patent No. 10-0512285 discloses a medicinal soap composition having an antimicrobial effect and a fungus containing fig fruit or leaf extract.

Agastache rugosa (Fisch. et Meyer) O. Kuntze, Kwakyeong) is a perennial herbaceous plant of Lamiaceae (Labiatae), which is dried and toppled over the ground. It is called Agastachis herba in Latin or Pogostemonis herba. In China, the same name Pogostemon cablin is commonly used together with Goguryeo as Pogostemonis herba (Latin name: Pogostemonis herba). It has been traditionally used as an herbal medicine (herbal medicine) such as anorexia, vomiting, dryness, diarrhea, and fever. The research on this has been carried out by combining plant extracts such as amino acids and Gwak (Japanese Patent Laid-Open No. 9-71527), and the like. Methylcavicol, rosmarinic acid, anethole, and other substances have been reported through studies on the composition of the wastewater.

The inventors of the present invention have been studying antioxidative, immunostimulating, whitening and skin antiaging agents originating from natural products, and have found that a composition containing hot water extract of gojiberry, fig and syrup contains antioxidant activity, immunity enhancement, whitening, It was confirmed that cytotoxicity was low while exhibiting a synergistic effect on inhibition of decomposition enzyme and collagen production promoting activity, thereby completing the present invention.

It is an object of the present invention to provide a composition for antioxidant, immunity enhancement, whitening, inhibition of collagenase, promotion of collagen production, and wrinkle improvement, which comprises hot water extract of goji berries, figs and syrups as an active ingredient.

The present invention provides an antioxidative composition comprising, as an active ingredient, an extract of gojiberries, figs and pomegranates.

In another aspect, the present invention provides a composition for enhancing immunity, which comprises an extract of goji berries, figs and pomegranate as an active ingredient. As used herein, the term "immunological enhancement" means increasing the immune response or activity of an in vivo immune system.

In another aspect, the present invention provides a composition for skin whitening comprising an extract of gojiberries, figs and pomegranate as an active ingredient. In the present invention, "skin whitening" means a result of inhibition of the production of melanin. More specifically, as the production of melanin is inhibited, symptoms caused by an increase in melanin such as stain, freckles, Can be understood.

In another aspect, the present invention provides a composition for inhibiting collagenase, which comprises an extract of goji berries, figs and bamboo shoots as an active ingredient.

In another aspect, the present invention provides a composition for promoting collagen production comprising an extract of gojiberries, figs, and chrysanthemum as an active ingredient.

In another aspect, the present invention provides a composition for improving wrinkles comprising an extract of gojiberries, figs and pearlescentes as an active ingredient.

When the extract is obtained by treating an extraction solvent, various extraction solvents may be used, and a polar solvent may preferably be used. Suitable polar solvents include purified water, alcohols (such as methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol) dimethyl-formamide) and DMSO (dimethyl sulfoxide). More preferably, the extraction solvent used in the present invention includes purified water, anhydrous or lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, And a mixed solvent of the lower alcohol and purified water. Most preferably, the extract of the present invention is a hot-water extract.

As used herein, the term "extract" is meant to be used in the art as a crude extract as described above, but broadly it also includes a fraction that further fractionates the extract. That is, the extract according to the present invention includes not only those obtained by using the above-described extraction solvent but also those obtained by additionally applying a purification process thereto. For example, the extract may be fractionated by passing the fraction through an ultrafiltration membrane having a cut-off value at a constant molecular weight, or by separation by various chromatographies (prepared for separation by size, charge, hydrophobicity, or affinity) Fractions obtained through various purification methods performed are also included in the above extract. In addition, the extract according to the present invention can be prepared in powder form by an additional process such as re-extraction, vacuum distillation, freeze-drying, spray drying or a combination thereof.

According to one embodiment of the present invention, the extract comprises (i) 5 to 20 parts by weight, or 7 to 12 parts by weight, or 10 to 15 parts by weight of goji berries; (ii) 5 to 20 parts by weight, or 7 to 12 parts by weight, or 10 to 15 parts by weight of figs; And (iii) from 60 to 90 parts by weight, or from 65 to 85 parts by weight, or from 70 to 80 parts by weight, respectively.

According to one embodiment of the present invention, the extract may be a mixture of extracts for each of high-fat goji berries, figs, and sashes at a weight ratio of 1: 1: 1, 2: 1: 1, or 2: 1: 2.

According to one embodiment of the present invention, the composition of the present invention comprises a pharmaceutically effective amount of the above-mentioned extract of Goji berries, figs and persimmon extracts of the present invention; And a pharmaceutically acceptable carrier. As used herein, the term "pharmaceutically effective amount" means an amount sufficient to achieve the efficacy or activity of the extract described above.

When the composition of the present invention is prepared from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers to be contained in the pharmaceutical composition of the present invention are those conventionally used in the present invention and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. In addition, the pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably administered orally.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on such factors as formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, And typical dosages range from 0.001-100 mg / kg on an adult basis.

Another aspect of the present invention provides a food composition comprising as an active ingredient an extract of Gojiberries, Figs, and Chrysanthemum of the present invention.

The food composition according to the present invention includes all forms such as functional food, nutritional supplement, health food and food additives. These types can be prepared in various forms according to conventional methods known in the art. For example, as a health food, the extracts of Goji berries, figs and chrysanthemums of the present invention can be prepared in the form of tea, juice and drink and then drunk, granulated, encapsulated and powdered. Furthermore, in addition to the extracts of Koji Berry, Figs and Chrysanthemum according to the present invention, known substances or active ingredients known to have immunosuppression, whitening, collagenase inhibition, collagen production promotion, . ≪ / RTI >

When the composition of the present invention is prepared with a food composition, it contains not only extracts of the above-mentioned high-fat berry, fig and berry, but also components which are usually added during the production of food, for example, proteins, carbohydrates, fats, Nutrients, flavoring agents, and flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors such as tau martin, stevia extract (e.g., rebaudioside A and glycyrrhizin), and synthetic flavors (saccharin, aspartame, etc.) may be used as flavorings. For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, .

In the food composition of the present invention, the preferred content of the extract of the present invention is 0.01 to 100% by weight or 0.01 to 50% by weight in the finally prepared food. In order to use the extract of the present invention as a food additive in the form of powder or concentrate, it can be used.

Another aspect of the present invention provides a cosmetic composition comprising an extract of Gojiberries, figs and syrups as an active ingredient.

The cosmetic composition according to the present invention may be prepared in any form conventionally produced in the art, and in this case, it may be prepared by including a carrier and the like conventionally used in the formulation of cosmetic composition. According to one embodiment, the carrier may comprise from about 1% to about 99.99% by weight, preferably from about 5% to about 99% by weight of the composition, based on the total weight of the cosmetic composition of the present invention.

According to one embodiment, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray May be formulated, but are not limited thereto. More specifically, when the composition of the present invention is used as a cosmetic composition, it may be formulated into cosmetic formulations selected from lotions, emulsions, creams, essences, gels, packs and cleansing creams; And a makeup cosmetic formulation such as a foundation.

When the cosmetic composition according to one embodiment is formulated into a paste, cream or gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, Zinc oxide and the like may be used. When the composition of the present invention is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydro Propellants such as carbon, propane / butane or dimethyl ether.

When the cosmetic composition according to one embodiment is formulated as a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl Benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the cosmetic composition according to one embodiment is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component; Suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

The composition of the present invention comprising the extract of Gojiberries, figs and chrysanthemum as an active ingredient achieves an excellent effect through synergy in antioxidation, immunity enhancement, whitening, suppression of collagenase, promotion of collagen production and wrinkle improvement .

Fig. 1 is a graph showing the antioxidative effect of hot water extract of Gojiberry, Fig.
Fig. 2 is a graph showing the cell survival rate of the hot-water extract of Gojiberry, Fig.
FIG. 3 is a graph showing the effect of hot water extract of goji berries, figs, and pomace on the proportion of TNF-a fraction of macrophages.
Fig. 4 is a graph showing the effect of hot water extract of goji berries, figs, and syrups on melanin production.
FIG. 5 is a graph showing an effect of inhibiting tyrosinase activity by hot-water extract of goji berries, figs and syrups.
6 is an electrophoresis result showing the effect of hot water extract of goji berries, figs, and syrups to inhibit keratinocyte-derived MMP-1 gene expression.
FIG. 7 is a graph showing the effect of the hot-water extract of Goji berries, figs, and syrups to promote type 1 procollagen production.
FIG. 8 is a graph showing the effect of the hot-water extract of Gojiberry, Fig.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the description. It is to be understood, however, that the scope of the present invention is not limited to these embodiments and that all changes, equivalents, and alternatives falling within the spirit and scope of the present invention are included . DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

Production Example 1

Production Example 1-1

10 g of distilled water was added to each of 50 g of goji berries, 50 g of figs and 50 g of sieve, and the mixture was heated at 95 ° C for 4 hours using a heating mantle equipped with a reflux condenser. The mixture was filtered, And dried to obtain respective extracts.

Production Example 1-2

The koji berry extract, the fig extract, and the bamboo extract of Preparation Example 1-1 were mixed at a weight ratio of 1: 1: 1 and 2: 1: 2, respectively.

Production Example 2

Production Example 2-1

14.93 g of goji berries, 9.29 g of figs and 75.78 g of bamboo shoots were mixed, and then 10 times of distilled water was added. The mixture was heated at 95 ° C for 4 hours using a heating mantle equipped with a reflux condenser, And lyophilized to obtain an extract.

Production example 2-2

The goji berries, figs, and syrups were extracted, filtered and concentrated as in Preparation Example 2-1, followed by spray drying to obtain an extract.

Experimental Example 1: Measurement of antioxidant activity

DPPH (2,2-diphenyl-1-picrylhydrazyl). After each sample was treated with DPPH (250 μM) (Positive control: Vitamin C, 50 μg / mL), the reaction was induced at 37 ° C for 30 minutes. The antioxidant activity was calculated from the OD value obtained by measuring the absorbance at 517 nm after completion of the reaction, and the results are shown in Fig.

As shown in Fig. 1, the extracts obtained by separately extracting hot water extract of gojiberries, figs, and syrups were mixed at a ratio of 1: 1: 1, and the mixture was mixed with 14.93, 9.29, and 75.78 parts by weight of gojiberry, The antioxidant activity was measured by extracting, lyophilization or spray drying. FIG. 1 shows that the method of extracting the mixture after mixing and extracting the goji berries, figs and syrups alone was more remarkable in terms of antioxidant efficacy.

Experimental Example 2: Measurement of cell viability

2-1. Cell culture

Mouse macrophage RAW264.7 cells were cultured in RPMI 1640 medium containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 10% FBS in a 100 mm cell culture dish at a density of 70-80% Lt; / RTI >

2-2. Cell viability measurement

MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphinyltetrazolium bromide) assay. The cultured mouse macrophage cell line RAW264.7 was adjusted to a concentration of 1 to 10 ⁶ cells / ml using RPMI 1640 medium, then inoculated in a 96 well plate and preincubated for 18 hours at 5% CO 2 and 37 ° C. Then, the medium was removed and the extracts were treated with medium containing the concentrations of 100, 200 and 400 ug / mL, respectively. After 24 hours, 10 μl of MTT solution (stock concentration: 5 mg / ml) was added and an additional reaction was induced for 3 hours. 100 μl of DMSO was added to each well for reaction termination and formazan crystal dissolution. The cell viability was calculated from the OD value obtained by measuring the absorbance at 570 nm of the amount of MTT reduced to formazan, and the result is shown in Fig.

As shown in FIG. 2, the extracts obtained by separately extracting hot water extracts of gojiberries, figs, and syrups were mixed at a ratio of 1: 1: 1, and the seeds were mixed at 14.93, 9.29, and 75.78 parts by weight of gojiberry, The cell viability was found to be in the range of 80 to 120% in the cells treated with the extract and lyophilized or spray dried. Based on the above results, it was confirmed that the extracts did not affect cell viability at the concentration range tested.

EXPERIMENTAL EXAMPLE 3 Effect of Extract Concentration on Immune Enhancement - Measurement of TNF-α production from macrophages

The cultured mouse macrophage cell line RAW264.7 was adjusted to a concentration of 1 to 10 ⁶ cells / ml using RPMI 1640 medium, inoculated on a 96-well plate, and preincubated for 18 hours at 5% CO ₂ and 37 ° C . After the medium was removed, the medium containing the extracts and the positive control (LPS, 100 ng / mL) at the concentrations of 100, 200 and 400 ug / mL was treated and cultured. Twenty-four hours later, the supernatant was transferred to another 96-well plate (100 l) and the concentration of TNF-α was measured according to manufacturer's instructions using an enzyme-linked immunosorbent assay (ELISA) kit from R & D Systems. .

As shown in FIG. 3, the extracts obtained by separately extracting hot water extracts of gojiberries, figs, and syrups were mixed at a ratio of 1: 1: 1, and the mixture was mixed with 14.93, 9.29, and 75.78 parts by weight of gojiberry, TNF-α production was measured in the extract, lyophilized or spray-dried group, and the highest level was confirmed in the experimental group after mixing. From the above results, it was confirmed that the method of extracting after mixing alone is more effective in enhancing the immunity enhancing effect than the method of separately extracting and mixing the goji berries, figs, and pears.

Experimental Example 4: Measurement of melanin production

4-1. Cell culture

B16-F10 cells, a mouse melanoma cell line, were cultured in a 100 mm cell culture dish using DMEM medium containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 10% FBS at a density of 70-80% Lt; / RTI >

4-2. Melanin concentration measurement

The mouse melanoma cell line B16-F10 cells were adjusted to a concentration of 1 × 10 ⁵ cells / ml using DMEM medium containing penicillin (100 IU / ml) and streptomycin (100 μg / ml) and 10% FBS Then, the cells were inoculated into a 96-well plate and pre-cultured for 18 hours at 5% CO ₂ and 37 ° C. The medium was then removed and cultured in the presence of 1600 ug / mL of each extract and a control (positive control: PTU (Phenylthiourea), 10 μM) containing medium and alpha-melanocyte-stimulating hormone (α-MSH). After 24 hours, the medium was removed and 1N NaOH was added to dissolve melanin. The melanin content was calculated by measuring the absorbance at 405 nm, and the results are shown in Fig.

As shown in Fig. 4, extracts obtained by separately extracting hot water extracts of goji berries, figs, and sake extracts were mixed at a ratio of 1: 1: 1 and the mixture of goji berries, figs, And melanogenesis in the freeze-dried or spray-dried group was confirmed to be similar to that of the positive control.

Experimental Example 5: Measurement of tyrosinase enzyme activity

The mouse melanoma cell line B16-F10 cells were adjusted to a concentration of 1 × 10 ⁵ cells / ml using DMEM medium containing penicillin (100 IU / ml) and streptomycin (100 μg / ml) and 10% FBS Then, the cells were inoculated into a 96-well plate and pre-cultured for 18 hours at 5% CO ₂ and 37 ° C. The medium was then removed and cultured in the presence of 1600 ug / mL of each extract and control medium plus alpha-melanocyte-stimulating hormone (α-MSH). After 10 minutes, the cells were disrupted using RIPA buffer, and the supernatant was separated by centrifugation at 14000 rpm for 20 minutes at 4 ° C. Then, 0.1% L-DOPA (L-3,4-dihydroxyphenylalanine) was added thereto, followed by incubation at 37 ° C for 3 hours. The tyrosinase enzyme activity was calculated by measuring the absorbance at 475 nm, and the results are shown in FIG.

As shown in FIG. 5, the extracts obtained by separately extracting hot water extracts of gojiberries, figs, and syrups were mixed at a ratio of 1: 1: 1, and the mixture of the sesame seeds, figs, and syrups were mixed at 14.93, 9.29, and 75.78 parts by weight, respectively Tyrosinase activity was measured by extracting, lyophilization or spray drying. The activity of the enzyme was decreased to a similar extent as the control group.

Experimental Example 6: Measurement of expression of MMP-1 gene derived from keratinocytes

6-1. Cell culture

Human keratinocyte HaCaT cells are cultured in 100 mm cell culture dishes at a density of 70-80% using DMEM media containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 10% FBS .

6-2. MMP-1 gene expression measurement

In order to investigate the level of expression of MMP-1 at the transcription level, each sample and control (positive control: Vitamin C, 50 ug / mL) were treated for a certain period of time and irradiated with UVB ultraviolet light. Total RNA was extracted using Trizol reagent . The extracted total RNA was amplified by PCR using first strand cDNA synthesis kit (Thermo scientific). The sense and antisense primer sequences of the target proteins used were prepared by reference to the existing literature and GAPDH was used as a control gene. PCR amplification was carried out using a Hipi PCR kit (Elpis biotech) containing 250 mM dNTP, 10 mM KCl, 50 mM KCl and 1.5 mM NgCl ₂ , and MMP-1 primers and control GAPDH primers Hipi PCR kit was used in 20ul. PCR was performed at 95 ° C for 45 seconds, annealing at 60 ° C for 45 seconds, and extension at 72 ° C for 1 minute. A total of 30 cycles were performed. The DNA amplified by PCR was electrophoresed on 1.5% agarose gel, and the intensity of the fractionated DNA bands was measured. The results are shown in FIG.

- MMP-1 forward 5'-ATT CTA CTG ATA TCG GGG CTT TGA-3 ', MMP-1 reverse 5'-ATG TCC TTG GGG TAT CCG TGT AG-

- glyceraldehyde 3-phosphate dehydrogenase (GAPDH) forward 5'-CCA GCC GAG CCA CAT CGC TC-3 ', GAPDH reverse 5'-TGA CCT TGG CCA GGG GTG CT-

As shown in FIG. 6, the extracts obtained by separately extracting hot water extracts of gojiberries, figs, and syrups were mixed at a ratio of 1: 1: 1, and the mixture of goji berries, figs, and syrups were mixed at 14.93, 9.29, and 75.78 parts by weight, respectively The expression of MMP-1 gene in the extract and freeze-dried or spray-dried group was confirmed to be decreased in gene expression.

Experimental Example 7: Measurement of collagen production ability derived from fibroblast

7-1. Cell culture

Human fibroblast HS68 cells were cultured in 100 mm cell culture dishes at a density of 70-80% using DMEM media containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 10% FBS .

7-2. Type 1 Procollagen Measurements

The cultured human fibroblast HS68 cells were adjusted to a concentration of 1 × 10 ⁶ cells / ml using DMEM medium containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 10% FBS , And inoculated on a 96-well plate and preincubated for 18 hours at 37 ° C and 5% CO ₂ . Then, the medium was removed and the extract-containing medium was simultaneously treated and cultured. After 24 hours, 100 μl of the supernatant was transferred to another 96 well plate, and the concentration of procollagen was measured according to the manufacturer's instruction using an enzyme-linked immunosorbent assay (ELISA) kit of Abcam. The results are shown in FIG.

As shown in FIG. 7, the extracts obtained by separately extracting hot water extracts of gojiberries, figs, and syrups were mixed at a ratio of 1: 1: 1, and the mixture of gosyberries, figs, and syrups was mixed at 14.93, 9.29, and 75.78 parts by weight The production of type 1 procollagen was measured by extraction, lyophilization or spray drying and the results were confirmed to be increased.

Experimental Example 8: Measurement of PPAR-α, δ activity derived from monkey kidney cells

8-1. Cell culture

Monkey kidney cell line, Cos-7 cells, was cultured in 100 mm cell culture dish at 70-80% density using DMEM media containing penicillin (100 IU / ml), streptomycin (100 μg / ml) and 10% FBS Lt; / RTI >

8-2. Type 1 Procollagen Measurements

Monkey kidney-derived cell line Cos-7 cells were adjusted to a concentration of 4 × 10 ⁶ cells / ml using DMEM medium, inoculated into a 24-well plate, and preincubated for 24 hours at 5% CO ₂ and 37 ° C. . Then, pFR-Luciferase (10 μg / ml) and pFR-PPAR (1.5 μg / ml) were introduced into the cells using Lipofectamine from Thermo Fisher and cultured for 24 hours. The medium was then removed and the phenol-free DMEM medium supplemented with each extract was treated at the same time for 24 hours. After incubation, cells were lysed with lysis buffer and reacted with substrate 1: 1. After the reaction, the absorbance was immediately measured with a luminometer. The results are shown in FIG.

As shown in Fig. 8, the extracts obtained by separately extracting hot water, goji berries, figs, and syrups were mixed with 14.93, 9.29, and 75.78 parts by weight of extracts, δ activity, it was confirmed that the activity was increased in mixed extract and mixed spray drying group. This shows a synergistic effect on the whitening and skin epidermal regeneration effects in the complex extracts rather than the efficacy of each single extract.

As can be seen from the results of the above Experimental Examples, the composition comprising the hot water extract of Koji Berry, the fig and the sake according to the present invention is effective for antioxidation, immune enhancement, whitening, collagenase inhibition, collagen production promotion, , Which is equivalent to that of the positive control, which is due to the synergistic action between the extracts of Gojiberry, Fig.

The above description is only illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. It should be noted that the embodiments disclosed in the present invention are not intended to limit the scope of the present invention and are not intended to limit the scope of the present invention. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

Claims (8)

An antioxidant composition comprising an extract of goji berries, figs and pomegranates.
A composition for enhancing immunity comprising an extract of Goji Berry, a fig and a pomegranate.
A composition for skin whitening comprising an extract of Goji berries, figs and pomegranates.
A composition for improving wrinkles comprising an extract of Goji berries, figs and pomegranates.
5. The composition according to any one of claims 1 to 4, wherein the extract is extracted with hot water.
[Claim 6] The composition according to claim 5, wherein the extract is a mixed extract of 5 to 20 parts by weight of goji berries, 5 to 20 parts by weight of figs, and 60 to 90 parts by weight of a sieving solution.
The composition according to claim 5, wherein the extract is prepared by extracting hot pepper, fig and syrup respectively with hot water and mixing in a weight ratio of 1: 1: 1, 2: 1: 1, or 2: 1: 2.
The composition according to any one of claims 1 to 4, wherein the extract is contained in an amount of 0.01 to 100 parts by weight based on 100 parts by weight of the total composition.

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