KR20180097102A - A cosmetic composition comprising fermented extract of coffee grounds - Google Patents

Abstract

The present invention relates to a whitening costmetic composition. Provided is a cosmetic composition containing a fermented extract of coffee ground obtained by inoculating a Bacillus spp. strain or a Schizosaccharomycesspp. strain or a Saccharomycesspp. strain.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition comprising a coffee ground fermented extract as an active ingredient,

The present invention relates to a cosmetic composition containing a coffee ground fermented extract as an active ingredient.

Skin is the primary barrier of the human body. It protects the internal organs of the body from external stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants, and plays an important role in the maintenance of body homeostasis such as body temperature control.

As the industry develops, environmental pollution becomes serious, and there is a growing interest in skin aging prevention and whitening. In particular, human skin constantly undergoes changes, the most representative of which is degradation of the skin caused by aging and a reduction in visual beauty. The final appearance of skin appearance as a result of skin aging is wrinkle formation, skin elasticity decrease, senile spot formation, and wrinkle formation is considered to be the most typical phenomenon.

Lipids, proteins, polysaccharides and nucleic acids, which are major components of the skin, can be oxidized to destroy skin cells and tissues, and the skin may eventually age. Particularly, oxidation of proteins can cause collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, etc., which are connective tissues of the skin, to be cleaved and hyperinflammation reaction. In the case of further deepening, mutation due to DNA mutation, Cancer induction can be promoted.

Therefore, it is necessary to protect the cell membrane by eradicating reactive oxygen species which are mediated by reactive oxygen species, ultraviolet ray irradiation, and inflammation reaction occurring during the metabolism of the body, regenerate damaged cells through active metabolism, and restore skin health desirable.

(Japanese Patent Laid-open No. 4-9320), hydroquinone (Japanese Patent Laid-open No. 6-192062), kojic acid (Japanese Patent Laid-open No. 56-7710), arbutin Rosinase inhibitory activity and was used as a whitening cosmetic. However, these ingredients are poor in stability among cosmetic formulations, may be decomposed to cause coloration or odor, and their safety or efficacy in vivo is unclear and their use is limited.

The cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

On the other hand, the coffee residue, which is extracted from coffee beans, is a large amount of wastes generated by the world over 6 million tons per year. However, there is a lack of utilization and treatment methods. Therefore, studies on the recycling of coffee beans have been actively carried out, and research has been conducted on the use of coffee beans as fuel, feed or fertilizer additives, as substrates for producing mushrooms, and as raw materials for biodiesel.

As a result of careful examination of the possibility and application of the coffee bean, the present inventors have found that the effective ingredient having skin whitening activity can be significantly increased by the fermentation process by the microorganism, and utilized it as a material for functional cosmetics.

Disclosure of Invention Technical Problem [8] The present invention has been made to solve the above-mentioned problems of the prior art, and an object of the present invention is to provide a cosmetic composition having not only functionality but also biostability.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a coffee ground fermented extract.

In one embodiment, the fermented extract is selected from the group consisting of Bacillus spp . , Schizosaccharomyces sp . spp . ), Or a strain of the genus Saccharomyces spp .

In one embodiment, the Bacillus subtilis strain may be Bacillus subtilis .

In one embodiment, the strain of the genus Schizosaccharomyces sp. May be a schizosaccharomyces pombe .

In one embodiment, the Saccharomyces sp. Strain may be Saccharomyces carlsbergensis .

In one embodiment, the cosmetic composition may be for skin whitening.

In one embodiment, the cosmetic composition is comprised of a softening agent, a nutritional lotion, a water cream, a nutritional cream, a massage cream, an essence, an ampoule, a gel, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, Lt; / RTI > may be formulated into one or more selected from the group.

Since the cosmetic composition according to one aspect of the present invention contains an active ingredient of a natural raw material, it is not only beneficial to skin health but also has excellent skin whitening effect.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

An aspect of the present invention provides a cosmetic composition comprising a coffee ground fermented extract as an active ingredient.

The coffee grounds are extracted residues produced in the process of producing coffee hot water extracts, which are estimated to produce over 6 million tons / year worldwide. (Saenger, M., Hartge, E. Werther, J. Ogada, T. and Siagi, Z. 2001. Combustion of coffee husks. Renew. Energ. 23: 103- 121.), a functional material source (Simoes, J., Madureira, P., Nunes, FM, Domingues, MR Vilanova, M. and Coimbra, MA 2009. Immunostimulatory properties of coffee mannans. : 1036-1043.), A biodiesel feedstock (Franca, AS, Oliveira, LS and Ferreira, ME 2009. Kinetics and equilibrium studies of methylene blue adsorption by spent coffee grounds. Desalination 249: 267-272. ought.

The coffee grounds contain various physiologically active ingredients. By inoculating and fermenting specific microorganisms, the whitening activity and the antioxidative effect can be remarkably increased.

The above-mentioned " comprising as an active ingredient " is intended to mean an effective amount of an effective amount capable of exhibiting a skin improving effect, such as collagen synthesis associated with a wrinkle-reducing effect, elasticity improvement or skin whitening, skin irritation alleviation, It can mean to do.

The above-mentioned "extract" refers to a solvent in which an effective ingredient contained in an extraction raw material has been transferred by contacting a solvent and an extraction raw material under specific conditions. If a substance is separated from a natural product, the extraction method, You can include them all regardless. For example, it may include extracts of components dissolved in a solvent from natural products by using water or an organic solvent, and specific components of natural products, such as oils obtained by extracting only specific components such as oil.

The extract may be obtained by drying the coffee bean after the coffee bean has been pulverized, or by various extraction methods known in the art such as a hot water extraction method and an ethanol extraction method.

Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol , And may be extracted with ethanol, preferably.

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

The extract is washed with water, dried and pulverized, and extracted and extracted with a solvent having a volume of 8 to 12 times the weight of the raw material for about 1 to 24 hours by a conventional method such as circulation extraction, pressure extraction, Followed by filtration. In addition, the extract can be obtained in powder form by an additional process such as vacuum distillation or freeze-drying.

The extract may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

The term " fermentation " means a process in which a microorganism decomposes an organic substance using an enzyme. It is known that a raw material that has undergone fermentation by the microorganisms has an effect of at least 2 times to a maximum of several tens of times greater than that before fermentation. Fermented metabolites contain large amounts of amino acids, organic acids and antioxidants that are beneficial to the skin, promoting skin metabolism and making skin texture resilient and smooth. In addition, as the fermentation process proceeds, the particles become smaller and the toxicity such as heavy metals is decomposed to increase the absorption rate and alleviate skin troubles and allergy side effects.

The above-mentioned " fermentation extract " is obtained by drying the extract naturally or by using an apparatus, specifically a rotary vacuum concentrator and a freeze drier, diluting the extract to a certain concentration in a solvent, preferably purified water, It can be prepared by inoculation and fermentation.

The coffee ground fermented extract can increase the skin care effect such as skin whitening and antioxidant effect by the interaction of the fermentation product of the fermentation product or the newly generated fermentation product in the fermentation process, .

The fermented extract may be selected from the group consisting of Bacillus spp . , Schizosaccharomyces spp . , Or Saccharomyces sp. spp . ) Strain. ≪ / RTI >

The Bacillus subtilis strain may be Bacillus subtilis .

The Bacillus subtilis is a gram-positive bacterium and is known to be a probiotic agent having endospore formation in soil and in water.

The above-mentioned ski-investigated caromyces subsp. Strain may be a Schizosaccharomyces pombe for skiing.

The above-mentioned ski-inspired Karamoissefombe is a cleavage yeast belonging to the ascomycetes, as well as Saccharomyces cerevisiae, a sprouting yeast, but is not closely related evolutionarily. The ski survey caromaiethsufombe is located in an evolutionary higher system than baking yeast and has more similarity with mammalian cells than baking yeast.

The Saccharomyces sp. Strain may be Saccharomyces carlsbergensis .

The saccharomyces carassbergensis is a lower fermented brewer's yeast isolated from a beer factory in Denmark and can efficiently decompose organic matter to synthesize high quality amino acids, nucleic acids, fatty acids, hormones, vitamins and various minerals.

The present inventors have found that the above-mentioned strains can effectively improve the skin whitening effect by effectively converting the active ingredient of the coffee bean and increasing the whitening active ingredient.

The cosmetic composition may be used for skin whitening. The "skin whitening" mentioned above is intended to mitigate the formation of various biomolecules such as melanin, redox hemoglobin, carotene and melanoid affecting the skin color, thereby alleviating skin pigmentation phenomena such as dullness, freckles and stains, It means an effect of alleviating redness and improving skin brightness and uniformity.

The cosmetic composition may be at least one selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Can be formulated.

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. The cosmetic composition may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Manufacturing example  :  Coffee shop  Preparation of fermented extract

Roasted coffee bean flour was purchased and used for the experiment. The water-soluble component was removed by extraction with water at 90 ° C for 30 seconds using a coffee maker, and the coffee bean powder in which the water-soluble component was removed was dried to remove moisture to prepare a coffee bean.

200 times volume of microorganism medium was added to the prepared coffee grounds and the fermentation was inoculated and fermented at a temperature of 30 ° C for 72 hours. The fermented product was filtered through a 500-mesh filter and a 0.2-μm filter, followed by freeze-drying to obtain a coffee ground fermented extract.

Example  And Comparative Example

To verify the skin whitening effect of the fermented extract, fermented extracts were prepared by varying fermentation strains as shown in Table 1 below, and examples and comparative examples were set.

division Fermentation strain Example 1 Bacillus subtilis Example 2 Schizosaccharomyces pumice Example 3 Saccharomyces carlsbergensis Comparative Example 1 Non fermentation Comparative Example 2 Lactobacillus Casei Comparative Example 3 Bifidobacterium bifidum Comparative Example 4 Monascus anka Comparative Example 5 Saccharomyces cerevisiae Comparative Example 6 Zymomonas mobilis

Experimental Example  1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

The samples of Examples and Comparative Examples were each suspended in purified water at a concentration (50 mg / L), and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added to each well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example  2: tyrosinase inhibitory activity test

The tyrosinase inhibitory activity was measured in vitro to compare the whitening effects of the examples and the comparative examples.

Each of the samples of the Examples and Comparative Examples was suspended at a concentration of 50 mg / L, and then 220 μL of 0.1 M phosphate buffer (pH 6.5), 20 μL of the sample solution and 20 μL of tyrosinase (1500 U / mL to 2000 U / mL) Respectively. 40 μl of a 1.5 mM tyrosinase solution was added and reacted at 37 ° C for about 15 minutes.

Absorbance was measured at 490 nm using an ELISA reader. A 0.1 M phosphate buffer (pH 6.5) was used instead of the sample solution.

As a control, kojic acid at a concentration of 50 μM was used, and tyrosinase inhibitory activity was calculated according to the following equation. The evaluation results are shown in Table 2 below.

[Mathematical Expression]

Tyrosinase inhibitory activity (%) = 100 x (1 - (absorbance of each test substance / absorbance of negative control))

division Tyrosinase inhibitory activity (%) Example 1 30.5 Example 2 32.7 Example 3 36.1 Comparative Example 1 11.5 Comparative Example 2 19.6 Comparative Example 3 15.7 Comparative Example 4 14.8 Comparative Example 5 17.5 Comparative Example 6 14.9 Positive control group 36.4

The samples of Examples 1 to 3 effectively inhibited the activity of tyrosinase and the tyrosinase inhibitory activity was evaluated to be comparable to that of the positive control (kojic acid).

On the other hand, the samples of Comparative Examples 2 to 6, which were fermented by other strains, showed relatively insufficient tyrosinase inhibitory activity, and the tyrosinase inhibitory activity of the coffee ground extract of Comparative Example 1, which had not undergone fermentation, .

Experimental Example  3: Measurement of polyphenol content

The content of polyphenols contained in the fermented extracts of Examples and Comparative Examples was measured. Polyphenol is excellent in the removal activity and antioxidant activity of radicals in the body, effectively inhibits tyrosinase and melanin production and activity, and thus has excellent skin whitening effect.

Quantification of polyphenol was performed according to AOAC. 1 mL of 1N-Folin-Ciocalteu phenol reagent reagent was added to 3 mL of the sample solution diluted 100 times with the samples of Examples and Comparative Examples. Add 0.2 mL of 1N HCl, add 1 mL of saturated Na ₂ CO ₃ solution, mix and leave at room temperature for 1 hour. After measuring the absorbance at 640 nm, the content of polyphenol was calculated by comparing the absorbance of the standard curve with the standard curve of tannic acid.

The contents of polyphenols contained in the samples of Examples and Comparative Examples were measured and the results are shown in Table 3 below.

division Polyphenol content (mg GAE / g DW) Example 1 6.42 Example 2 5.57 Example 3 5.32 Comparative Example 1 2.24 Comparative Example 2 3.34 Comparative Example 3 3.75 Comparative Example 4 3.57 Comparative Example 5 4.01 Comparative Example 6 3.86

The polyphenol contents of the samples of Examples 1 to 3 were evaluated to be significantly higher than those of Comparative Examples 1 to 6. In particular, the sample of Comparative Example 1 which did not undergo fermentation contained a relatively small amount of polyphenol.

The results showed that Bacillus subtilis, skiing caromaiethsum pombe, and Sakaromyces carlsbergensis produced a large amount of polyphenol through fermentation process for coffee beans, and antioxidant activity and whitening activity of coffee bean extract were significantly Suggesting that it can be increased.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (7)

A cosmetic composition comprising a coffee ground fermented extract as an active ingredient. The method according to claim 1,
The fermented extract may be selected from the group consisting of Bacillus spp . , Schizosaccharomyces spp . , Or Saccharomyces sp. spp . ). ≪ / RTI > 3. The method of claim 2,
Wherein the Bacillus subtilis strain is Bacillus subtilis . 3. The method of claim 2,
The cosmetic composition according to claim 1, wherein the Schizosaccharomyces sp. Strain is a ski-examined Schizosaccharomyces pombe . 3. The method of claim 2,
Wherein the Saccharomyces sp. Strain is Saccharomyces carlsbergensis . 6. The method according to any one of claims 1 to 5,
A cosmetic composition for skin whitening. 6. The method according to any one of claims 1 to 5,
Cosmetics formulated with at least one selected from the group consisting of softening longevity, nutritional lotion, moisture cream, nutritional cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Composition.