KR20180065994A - External composition for skin containing germinated seeds of ginseng extract - Google Patents

Abstract

The present invention relates to a composition for skin external preparation, containing an extract of germinated ginseng seeds. More particularly, the present invention relates to the composition for skin external preparation, wherein the composition contains the extract of germinated ginseng seeds and thus has excellent effects in skin protection, anti-aging and anti-wrinkling, skin elasticity improvement, whitening, skin moisturizing, prevention and improvement of dry skin diseases such as atopic diseases and the like, sebum controlling, anti-inflammatory, prevention or alleviation of skin troubles such as acne and the like, skin complexion improvement, keratin removal, hair loss prevention, anti-dandruff, improvement of shine and softness of hair, improvement of shape and elasticity of the face or body, and improvement of lines of the face or body.

Description

[0001] The present invention relates to an external composition for skin containing ginseng seed extract,

The present invention relates to a composition for external application for skin containing ginseng ginseng seed extract, and more particularly to a composition for external application for skin which contains ginseng ginseng seed extract to excellently improve the skin's overall effect.

In recent years, a composition for external application for skin such as cosmetics using natural products has been developed in order to solve the above problems in moisturizing, whitening, anti-aging effect and the like while reducing side effects of skin caused by various chemical substances and the like. Accordingly, the present inventors have studied the possibility of application as an external preparation for skin to improve overall skin in various natural products not yet widely known.

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ganoderma and has been used as a herbal medicine in Korea, China and Japan for over 2,000 years to prevent disease and prolong its life. The known potency and effects of ginseng are as follows: action on the central nervous system, anti-cancer and anti-cancer activity, immune function regulation, anti-diabetic effect, liver function enhancement, cardiovascular disorder improvement and anti-arteriosclerosis effect, Antioxidant activity, antioxidant activity, and antioxidant activity of the present invention. Ginsenoside, which is a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. However, ginsenoside content and composition vary greatly depending on the site such as ginseng roots, ginseng leaves, and ginseng fruit .

Recently, consumers' interest in natural cosmetics has been increasing, and at the same time, many cosmetics utilizing herbal materials have been released, and ginseng has been studied as a plant material having important skin effect of cosmetics. However, most studies using only ginseng root extract or ginsenoside components of ginseng roots and ginseng polysaccharides are performed.

Korean Patent Publication No. 2009-0130801

The inventors of the present invention have repeatedly and variously conducted experiments to confirm that the ginseng seed extract can provide a safe and sufficient protection and improvement effect to the skin. While researching, it has been found that the ginseng seed extract does not germinate The present invention has been accomplished based on the discovery that the extract has better skin protection and improvement effects. In addition, the present inventors have found that ginseng ginseng seed extract extracted by subcritical extraction method is more excellent in skin protection and improvement effect, and completed the present invention.

Accordingly, it is an object of the present invention to provide a composition for external application for skin having an excellent skin protecting and improving effect by containing germinated ginseng seed extract as an active ingredient. It is another object of the present invention to provide a composition for external application for skin which is useful for protecting and improving skin by containing an effective ingredient of a germinated ginseng seed extract extracted by a subcritical extraction method.

To achieve the above object, the present invention provides a composition for external application for skin containing germinated ginseng seed extract.

More specifically, the ginseng seed extract used in the present invention is extracted from germinated seeds of ginseng. Ginseng seeds germinated more than the germination state before the ginseng contains more essential ingredients needed for life phenomenon, can provide more powerful effects.

The germination ginseng seed can be germinated by culturing the ginseng seed in a clean water at a temperature of 20 ° C to 25 ° C. At this time, it is preferable that ginseng seeds are germinated by scratching their husks. It is preferable that ginseng seeds are put into water and then cultured for 7 to 10 days after watering once or twice a day. It is good to place in daylight where daylight is available.

The method for producing germinated ginseng seeds used in the present invention is not particularly limited, and it is preferable to use a method of producing germinated ginseng seeds by extracting ginseng seeds, extracting the ginseng seeds from the ginseng seeds, or extracting the extracts, And the chemical substance itself exhibiting the main effect contained in the extract.

In addition, the ginseng seed extract is preferably extracted with Subcritical Water Extraction. In conventional supercritical extraction methods, the density of supercritical fluids is similar to that of liquids, and viscosity and diffusion coefficients are similar to those of gases. Therefore, when a supercritical fluid is used as an extraction solvent, it can be easily penetrated into a micro-particle of a plant and has an appropriate molecular density, so that a desired substance extract can be obtained by surrounding molecules of a desired substance and coming out. At this time, as a substance which can be used as an extraction solvent by making it into a supercritical state, it should first be non-toxic, and since the supercritical state is high temperature / high pressure, there is no danger of explosion and excellent dissolving power should be used as an extraction solvent. Once these conditions are met, any substance can be made into a supercritical fluid for extraction.

On the other hand, the subcritical technology used in the present invention is used as an extraction solvent in a state in which the solvent does not exceed the critical point. Depending on what solvent is selected, the subcritical technology can achieve the same performance as supercritical extraction. Use water. Since supercritical carbon dioxide can not extract polar substances, organic solvents should be used together as an auxiliary solvent. However, supercritical carbon dioxide can extract both nonpolar and polar substances because of its superior extraction ability and range of operating conditions. Do. In addition, in the case of extraction with subcritical water, a hydrolysis reaction can also proceed.

According to this extraction method, the extract of ginseng seed can be extracted in a short time using the change of dielectric constant (e) of water according to temperature and pressure in a solid or semi-solid sample. Therefore, extraction of ginseng seeds by subcritical extraction method is faster than extraction by conventional solvent extraction method, and since water is used as an extraction solvent, it is harmless to human body and environment because there is no residual organic solvent or heavy metal. On the other hand, since the process proceeds to the low-temperature process, the activity of the extract is maintained and the thermo-denatured substance can be extracted, and the process itself can easily separate and recover the extract, thereby reducing the operation cost and recycling the solvent. It is also advantageous to obtain a useful substance selectively according to extraction conditions such as temperature and pressure.

The ginseng seed extract according to the subcritical extraction method used in the present invention can be specifically prepared as follows. After preparing the ginseng seed, the ginseng seed is decomposed at a pressure of 50 to 200 MPa, preferably 100 MPa by using a subcritical extractor to extract the active ingredient. The effective components are not sufficiently extracted at a pressure of less than 50 MPa and the extraction of the effective component is less than the pressure at a pressure exceeding 200 MPa. After extraction, the filter is filtered once with a filter having a pore size of 1.2 mu m, once with a filter having a pore size of 0.45 mu m, and once with a filter having a pore size of 0.2 mu m to remove impurities and microorganisms.

The composition of the present invention may contain the ginseng seed extract in an amount of preferably 1 to 50% by weight based on the total weight of the composition. If the content of the active ingredient is less than 1% by weight, it is difficult to expect the skin effect of the ingredient. If the content exceeds 50% by weight, the efficacy of the ingredient is not greatly increased.

The composition of the present invention can be used as an external preparation for skin to protect or generally improve the skin.

More particularly, the composition according to the present invention may be for skin damaging and skin protection. It is possible to inhibit or prevent the damage of skin cells caused by active oxygen or ultraviolet rays by containing germinated ginseng seed extract having an effect of inhibiting the production of active oxygen and inhibiting oxidative damage caused by active oxygen, This can protect your skin. The skin may be scalp or hair.

In addition, the composition according to the present invention can prevent or ameliorate skin aging or wrinkles by inhibiting skin damage caused by active oxygen or ultraviolet rays, promoting collagen production or inhibiting biosynthesis of matrix metalloproteinase (MMP-1) And the elasticity of the skin can be improved.

The composition according to the present invention can be used for skin whitening by inhibiting tyrosinase activity and inhibiting the production of melanin or by whitening the pigment deposited on the skin.

The composition according to the present invention promotes the differentiation of keratinocytes, thereby facilitating the elimination of keratinocytes and the differentiation or keratinization of epidermal cells, which is a process of producing new keratinocytes, thereby effectively removing keratin. Therefore, . In addition, the composition according to the present invention can keep the amount of moisturizing factor and lipid in the stratum corneum normally and maintain the transglutaminase expression by maintaining the turnover rate of the stratum corneum in the normal range. This promotes the differentiation of keratinocytes and maintains skin moisture, thereby enhancing skin moisturizing and skin barrier function, thereby preventing or treating skin dryness diseases such as atopy and psoriasis.

In addition, the composition according to the present invention suppresses excess sebum secreted when applied to the skin, reduces active oxygen and promotes collagen synthesis to reduce pores, and suppresses skin troubles by reducing the expression of inflammatory factors It can be used as a composition for external application for skin for reducing pores, controlling sebum and improving the symptoms of skin troubles, and further preventing or improving acne, enlargement of pores and seborrheic dermatitis due to excessive secretion of sebum.

Meanwhile, the composition according to the present invention can prevent or ameliorate inflammation by inhibiting biosynthesis of cyclooxygenase-2 (COX-2), thereby alleviating skin troubles including acne. In addition, excellent antioxidant power can prevent the generation of skin irritation. Here the skin contains the scalp.

Meanwhile, the composition according to the present invention may be used for improving the color of skin including the scalp or hair, and may be used for preventing hair loss, for anti-dandruff or for improving the gloss or softness of hair.

Furthermore, the composition according to the present invention can promote the differentiation of adipocytes, thereby improving the volume and elasticity of the face and the body. When the composition for external application for skin according to the present invention is applied to areas where the volume and the elasticity of the face and the body are required to be improved, volume and elasticity are enhanced. The area where volume and elasticity are required to be increased is the area that is depressed due to aging have. For example, when the composition according to the present invention is applied to a depressed ball, the volume may be enhanced by promoting differentiation of adipocytes. Another example of a negative that requires the improvement of volume and elasticity is the chest and the buttocks. When the composition according to the present invention is applied to the chest and the buttocks, the chest and buttocks are resilient and bulky.

In addition, the composition according to the present invention promotes the differentiation of adipocytes by enhancing the volume and elasticity of the face and body, promoting the biosynthesis of collagen and inhibiting collagen degradation, Can improve the damaged face and line of the body.

The composition of the present invention contains ginseng ginseng seed extract or germination ginseng seed extract extracted by subcritical extraction method, thereby providing skin protection and improvement effect superior to compositions containing common ginseng seed extract.

Furthermore, the composition of the present invention can be used not only for protecting skin but also for preventing and improving skin dryness diseases such as anti-aging, anti wrinkle, skin elasticity improvement, whitening, skin moisturizing and atopy, sebum control, anti- It is possible to provide an excellent effect in preventing or improving symptoms, exfoliation, improvement in skin color, prevention of hair loss, anti dandruff, improvement of gloss and softness of hair, volume and elasticity enhancement of face or body, .

Accordingly, the composition of the present invention can be used in various fields in the field of cosmetics or medicine.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the moisture content increase rate of a composition according to an embodiment of the present invention and a comparative example. FIG.
FIG. 2 is a graph showing the TEWL (water hardness loss) measurement results of a composition according to an embodiment of the present invention and a comparative example.

Hereinafter, the present invention will be described with reference to the following examples. The examples are intended to further illustrate the present invention and are not intended to limit the scope of the present invention.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope of the invention.

[Example 1] Ginseng ginseng seed extract

Ginseng seeds germinated in 70% pure water and 30% alcohol are left at 25 ℃ for 6 hours to extract the active ingredients. After the extraction, the filter is filtered once with a filter having a diameter of 3 μm and once with a filter having a filter having a diameter of 1 μm to remove foreign matter. Finally, microorganisms are removed with a filter to obtain germination ginseng seed extract.

[Example 2] Gout subcritical ginseng seed extract

Ginseng seeds germinated are extracted with active ingredient for 4 hours at 40 ° C and 20 MPa using a subcritical extraction device. After extraction, the filter is filtered once with a filter having a 1.2 μm gap, once with a filter having a 0.45 μm gap and once with a filter having a 0.2 μm gap to remove germs and microorganisms to obtain germination ginseng seed extract.

[Comparative Example] The ginseng seed extract

Ginseng seeds that are not germinated are put into 70% purified water and 30% alcohol and left at 25 ℃ for 6 hours to extract active ingredients. After the extraction, the filter is filtered once with a filter having a diameter of 3 μm and once with a filter having a filter having a diameter of 1 μm to remove foreign matter. Finally, microorganisms are removed with a filter to obtain a general ginseng seed extract.

[Test Example 1] Inhibition of active oxygen production

Antioxidant effect was investigated by comparing the ability to remove reactive oxygen species (ROS) from cells by ultraviolet (UV) irradiation. As a positive control, trolox (Aldrich Co.), which is generally used to compare antioxidative effects, was used. In addition, the test group was not used as a control group. (UVB 30 mJ / cm ² ) was applied to a human HaCaT keratinocyte monolayer culture system (PBoukamp, German Cancer Research Center, Heidelberg. German), and then the germinated ginseng seed Ginseng seed extract of Example 2 and non-germinated ginseng seed extract of Comparative Example were treated as shown in Table 1, respectively. Then, the amount of ROS was measured with a fluorescent plate reader using DCFH-DA (2 ', 7'-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc.). The inhibition ratio of ROS formation was calculated as a ratio to the control group treated with ultraviolet light, and the results are shown in Table 1.

Test substance ROS generation inhibition rate (%) Test substance ROS generation inhibition rate (%) Control group 0 Trolox 0.1 mM 42.7 Example 1 (10 ppm) 33.2 Example 1 (1 ppm) 17.5 Example 2 (10 ppm) 39.8 Example 2 (1 ppm) 21.3 Comparative Example (10 ppm) 19.9 Comparative Example (1 ppm) 11.2

The germinated ginseng seed extract prepared according to Example 1 and the germination germination seed extract of Example 2 were compared with the control group in the human HaCaT keratinocytes monolayer culture system in the human HaCaT keratinocytes monolayer culture system, It shows an effect of significantly erasing oxygen. This is an excellent activity similar to that of trolox used as an activity index of antioxidants. However, the non-germinated general ginseng seed extract of Comparative Example shows a less active oxygen scavenging effect than the germinated ginseng seed extract of the present invention. That is, the germinated ginseng seed extract prepared according to Example 1 of the present invention and the germination ginseng seed extract of Example 2 are more effective in eradicating active oxygen which is a cause of general skin damage, so that the skin including scalp and hair It is possible to confirm that it has an effect of preventing wrinkle formation, lowering of elasticity and pigmentation, and the like.

[Test Example 2] Inhibitory activity against DNA oxidative damage by single cell gel electrophoresis (SCGE, Comet assay)

After culturing human normal fibroblasts (their primary cultures) for 24 hours, germinated ginseng seed extract prepared according to Example 1, germination ginseng seed extract of Example 2, non-germinated ginseng seed extract of Comparative Example and positive The control group, vitamin E, was treated at each concentration, treated with H ₂ O ₂ (final concentration 10-3M) after 12 hours, and stained with electrophoresis, and observed with a fluorescence microscope. At this time, the length (um) of the tail, which is the result of DNA cleavage in each of 25 cells, was analyzed using an image analyzer, KOMET 3.1 (Kinetic Imaging, England) Respectively.

Test material tail length (탆) Inhibition rate (%) Test substance Tail length (탆) Inhibition rate (%) Control group 121.1 0.0 Vitamin E (10ppm) 68.9 43.10 Example 1 (10 ppm) 73.3 39.47 Example 1 (1 ppm) 91.2 24.69 Example 2 (10 ppm) 71.1 41.29 Example 2 (1 ppm) 88.4 27.00 Comparative Example (10 ppm) 95.2 21.39 Comparative Example (1 ppm) 103.1 14.86

In the results of Table 2, the germinated ginseng seed extract prepared according to Example 1 by cell gel electrophoresis and the germination ginseng seed extract of Example 2 were superior to the non-germinated ginseng seed extract of Comparative Example in oxidative DNA Inhibitory activity, similar to vitamin E, a positive control. Therefore, it can be confirmed that the ginseng seed extract according to the present invention acts as a DNA oxidative damage inhibitor which inhibits DNA single strand break by hydroxyl radical (.OH). This is an important protective effect for protecting the genes of cells of the skin including the scalp or hair.

[Experimental Example 3] Effect of promoting the production of procollagen

The procoagulant activity of the germinated ginseng seed extract prepared in Example 1 and the germination ginseng seed extract of Example 2 was compared with that of the non-germinated ginseng seed extract of vitamin C, which is a positive control, and the ginseng seed extract of Comparative Example. Pro-collagen is a collagen-inducing substance that is necessary for collagen production and anti-aging. Vitamin C is known to be an essential ingredient in the synthesis of collagen.

The test was carried out by placing human fibroblasts at 5,000 cells / well in a 96-well microtiter plate containing 2.5% fetal bovine serum-containing DMEM (Dulbecco's Modified Eagle's Media) And cultured until it grew to about 90%. Thereafter, the cells were cultured in a serum-free DMEM medium for 24 hours, treated with 10 ^-4 molar concentrations of each of the above Examples 1 and 2, Comparative Example and vitamin C dissolved in a serum-free DMEM medium for 24 hours, Collecting. After 24 hours, the amount of procollagen liberated in the medium was measured using a procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan).

The degree of procollagen production in the untreated group was taken as 100 and the degree of procollagen production in the group treated with the test substance was determined. The results are shown in Table 3 below.

Test substance Procollagen production (%) Untreated group 100 Vitamin C 120 Example 1 117 Example 2 119 Comparative Example 104

The higher the degree of production of procollagen, the higher the degree of production of collagen, and thus the generation of skin wrinkles can be prevented. Table 3 shows that the germinated ginseng seed extract prepared according to Example 1 and the germination ginseng seed extract of Example 2 are in vitro synthesized from vitamin C and procollagen which are known to be essential components for collagen synthesis But the non-germinated ginseng seed extract of Comparative Example had a lower degree of promotion of pro-collagen production than those of Examples 1 and 2. Thus, it can be seen that Examples 1 and 2 according to the present invention effectively promote collagen production, thereby contributing to the production of collagen in the skin, as well as an anti-wrinkle effect as well as an anti-aging effect.

[Test Example 4] Whitening effect test (measurement of inhibitory effect on melanin formation using mouse pigment cells)

The ginseng seed extract prepared in Example 1 and the germination ginseng seed extract of Example 2 were measured for their ability to inhibit melanogenesis by comparing the ginseng seed extract of the Comparative Example and the hydroquinone as a positive control.

The cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 nM 12-O-tetradecanoyl (10 μM) Acetate, and 1 nM cholera toxin at 37 ° C in the presence of 5% CO ₂ . The cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 10 ⁵ cells / well (cells / well) in a 24-well plate. And cultured. As test materials, hydroquinone was used at a concentration of 10 ppm in each of Examples 1 and 2 and Comparative Examples. At this time, the hydroquinone was used as a positive control. Then, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. The rate of inhibition of melanin formation was calculated according to the following formula (1) Dooley's method).

Test substance Melanin production inhibition rate (%) Untreated group 100 Hydroquinone (positive control) 41.1 Example 1 36.1 Example 2 38.7 Comparative Example 18.3

As shown in Table 4, the germinated ginseng seed extract prepared according to Example 1 of the present invention and the germination ginseng seed extract of Example 2 exhibited a degree of inhibition of melanin formation similar to that of hydroquinone, which is a known whitening substance, . And the inhibition rate of malalanin production is superior to that of the non-germinated ginseng seed extract of the comparative example. Thus, it can be seen that the composition of the present invention is excellent in whitening effect by effectively inhibiting the production of melanin in the skin.

[Test Example 5] Skin moisturizing test (Differentiation induction test of human keratinocytes)

The ginseng seed extract prepared according to Example 1 of the present invention and the germination ginseng seed extract of Example 2 were tested for their skin barrier function and skin moisturizing ability using absorbance.

First, human keratinocytes cultured in primary culture were placed in a culture flask and adhered to the bottom. Then, the cells of Examples 1 and 2 and Comparative Examples were added to the culture solution prepared as shown in Table 5 below, The cells were cultured for 5 days until they grew to about 80%. The cells were harvested and washed with PBS (phosphate buffered saline). The cells were washed with 10 mM Tris-HCl buffer (Tris-HCl buffer, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) , pH 7.4), sonication was performed for 3 minutes, and the mixture was boiled for 10 minutes. This was centrifuged at 1200 rpm for 30 minutes, and the separated precipitate was suspended in 1 ml of PBS and the absorbance at 340 nm was measured.

Separately, a part of the solution after the sonication was taken to measure the protein content, and was used as a standard for evaluation of the degree of cell differentiation. The test results are shown in Table 5 below, in which low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were used as negative / positive control groups and low calcium concentration test substances were added.

Test substance Concentration used The ability to differentiate in keratinocytes (%) Control group Low Ca < ^{2 +} & gt ^; (0.03 mM) 100 Hign Ca < ^{2 +} & gt ^; (1.2 mM) 206 Example 1 0.1 ppm 178 0.05 ppm 151 Example 2 0.1 ppm 183 0.05 ppm 166 Comparative Example 0.1 ppm 142 0.05 ppm 120

As shown in Table 5, when the amount of CE (cornified envelope) produced during keratinocyte differentiation was measured to compare cell differentiation promoting effects, The germinated ginseng seed extract prepared according to Example 1 of the present invention and the germination ginseng seed extract of Example 2 not only promoted differentiation in keratinocytes but also showed superior efficacy to the ginseng seed extracts not germinated in Comparative Example . Accordingly, it can be seen that the composition according to the present invention enhances the skin barrier function and improves the skin moisturizing ability.

[Production Examples 1 and 2, Comparative Production Examples 1 and 2]

The compositions containing the extracts prepared in Examples 1 and 2 and Comparative Examples were prepared by mixing in a conventional manner according to the composition shown in Table 6 below.

Compounding ingredient Production Example 1 Production Example 2 Comparative Preparation Example 1 Comparative Production Example 2 Purified water To 100 To 100 To 100 To 100 Example 1 0.1 - - - Example 2 - 0.1 - - Comparative Example - - - 0.1 Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 Preservative, incense Suitable amount Suitable amount Suitable amount Suitable amount Triethanolamine 0.1 0.1 0.1 0.1

(Unit: wt%)

[Test Example 6] Measurement of skin moisture persistence

To measure the skin moisture change and the sustainability at the time of one application, the moisture increase amount of each of Preparation Examples 1 and 2 and Comparative Preparation Examples 1 and 2 prepared by a conventional method according to the composition of Table 6 was measured using a corneometer Respectively.

First, 25 women aged 30 years or older were selected and washed with running water in flowing water. The test area was marked with a circle 1.5 cm in diameter at a distance of 5 cm from the wrist. The moisture content of the test area was measured by the following method using a moisture analyzer (CM825, Courage & Khazaka, Germany) after waiting for 30 minutes in a constant temperature and humidity chamber maintained at 23 ± 1 ° C and 45 ± 5% RH.

<Measurement method of moisture meter>

The amount of water present in the epidermis of the skin was measured by measuring the degree of ion of water using a sensor, numerically calculating the amount of moisture, and then measuring the moisture content.

1. A probe of a corneometer is placed on the skin area to be measured.

2. When the probe is pressed against the skin, the skin's electrical conductivity is quantified and displayed on the screen.

3. Measurement was repeated with different measurement sites.

4. After one measurement, the sensor was wiped with a tissue such as a kim wipe and then re-measured.

Thereafter, the same preparations of Preparation Examples 1 and 2 and Comparative Preparations 1 and 2 were applied to both test strips on both test strips, followed by 30 minutes, 1 hour, 2 hours, 4 hours, and 6 hours The moisture content of the test site was measured. The water content increase rate is a calculated value relative to the state before application of Production Examples 1 to 2 and Comparative Production Examples 1 and 2, and the average results are shown in Table 7 and FIG.

Before application 30 minutes 1 hours 2 hours 4 hours 6 hours Control group
(Mud) 26.20 26.11 26.21 26.15 26.19 26.17 Production Example 1 26.22 42.23 40.44 38.72 36.94 34.57 Production Example 2 26.17 42.81 41.51 40.17 38.93 36.66 Comparative Preparation Example 1 26.19 29.20 27.55 26.12 25.6 25.1 Comparative Production Example 2 26.21 37.21 36.22 33.14 29.25 27.22

As can be seen from Table 7 and FIG. 1, when Preparation Examples 1 and 2 containing germinated ginseng seed extract were applied, the prescription of Comparative Preparation Example 2 containing a common ginseng seed extract was applied to the skin It can be seen that the high moisture content lasts for a long time. In particular, in the case of Preparation Example 2 using ginseng extract of ginseng subcritical ginseng, as shown in Fig.

[Test Example 7] Measurement of skin moisturizing effect

TEWL (Trans Epidermal Water Loss) was measured to measure long-term skin moisturizing effect. At this time, tests were carried out using the same Production Examples 1 and 2 and Comparative Production Examples 1 and 2 as in Test Example 6. [

First, 30 women aged 30 years or older were allowed to apply Preparation Examples 1 and 2 and Comparative Preparations 1 and 2 twice a day for 4 weeks on both cheeks, 2 weeks after application, 4 weeks after application, TEWL (Trans Epidermal Water Loss) was measured by the following method at constant temperature and humidity (24 ° C, 40% relative humidity).

<TEWL measurement method>

The amount of moisture evaporating from the skin was calculated according to the area and time, and the amount of water evaporated was read and quantified with the temperature and humidity electronic sensor to measure the moisture of the skin.

1. Environmental conditions: The temperature and humidity were kept constant and only the minimum number of persons other than the measurer and the subject were left. At this time, the air flow was blocked as much as possible.

2. Place the TEWL probe on the subject's measurement site.

3. When placing the probe on the measurement site, place it lightly so that excessive force is not applied to the skin to leave marks on the skin, and make an angle of 90 degrees with the ground.

4. The value at which the TEWL value of the skin becomes constant as time passes is measured for about 1 to 3 minutes.

5. Handle the probe as far as possible so that the temperature of the measurer is not transmitted.

6. The amount of water evaporated per hour per area (g / m ² / h) was calculated.

The moisture resistance was compared with the measured TEWL, and the results are shown in Fig. As can be seen from FIG. 2, in the case of Production Examples 1 and 2, the amount of moisture evaporation was significantly reduced as compared with Comparative Production Example 2. That is, in Comparative Preparation Example 2 using common ginseng seed extract 2 weeks later, the skin moisture content was reduced to about 104%, but in Production Examples 1 and 2 of the present invention, the moisture content was maintained at over 125%. In particular, in the case of Preparation Example 2 using ginseng extract of ginseng subcritical ginseng, about 120% of the skin moisture was retained even after 4 weeks, and thus it was found that it has excellent skin moisturizing power.

[Test Example 8] Confirmation of skin elasticity improving effect

In order to confirm the skin elasticity improvement effect in humans, the moisture increase was evaluated using a corneometer.

110 healthy women from 30 to 40 were compared with the preparation examples 1 and 2. The nutritional creams were applied to the left side of the face for one week for 12 weeks for each of the four groups of preparation examples 1 and 2, 575, C + K Electronic Co., Germany). The results are shown in Table 8 below. The results in Table 8 are reported as ΔR8 (R8 (left) -R8 (right)) of Cutometer SEM 575, where the R8 value indicates the nature of the viscoelasticity of the skin.

Experimental product Skin elasticity effect Production Example 1 69 Production Example 2 73 Comparative Preparation Example 1 52 Comparative Production Example 2 58

As shown in Table 8, Production Examples 1 and 2 containing the germinated ginseng seed extract of the present invention are further improved in skin elasticity as compared with the group treated with Comparative Production Example 2 containing germinated seed extracts which are not germinated . Therefore, it can be confirmed that the composition containing germinated ginseng seed extract of the present invention is very effective for improving skin elasticity.

[Test Example 9] Confirmation of skin wrinkle improving efficacy

In order to confirm the wrinkle-improving effect of the composition of the present invention on humans, the above Production Examples 1 and 2 and Comparative Production Examples 1 and 2 were used.

In order to confirm the wrinkle-improving effect of the above-mentioned Production Examples 1 and 2 and Comparative Production Examples 1 and 2, the following evaluation was made. 110 healthy volunteers of 40 healthy women were treated with the nutrient cream once daily for 12 weeks for each of the four groups of Production Examples 1 and 2 in comparison with those of Production Examples 1 and 2. The replicas were then drained using silicone to remove wrinkles The state was measured with a visiometer (SV600, Courage + Khazaka electronic GmbH, Germany) and the images were analyzed. The results are shown in Table 9 below. The values in Table 9 below are the average values obtained by subtracting the pre-application parameter values from the respective parameter values after 12 weeks of application.

After 8 weeks of use
Clinical outcome R1 R2 R3 R4 R5 Production Example 1 41 43.2 45 15.9 11 Production Example 2 33 32.8 36 12.4 7 Comparative Preparation Example 1 69 68.6 76 31.2 22 Comparative Production Example 2 58 58.1 63 26.5 18

R1: Difference value between the maximum value and the minimum value of the wrinkle contour line R2: The wrinkle contour line is arbitrarily divided into 5 squares,

R3: the highest value of R1 divided by 5

R4: Average value obtained by subtracting the value of each apex and valley from the baseline of the wrinkle contour line

R5: Difference between the baseline of the wrinkle contour line minus the outline of each wrinkle

As shown in Table 9, Production Examples 1 and 2 containing the germinated ginseng seed extract of the present invention were found to have excellent skin wrinkle-reducing effect as compared to Comparative Preparation Example 2 containing ginseng seed extracts not germinated there was.

[Test Example 10]

In order to evaluate the skin blood circulation promoting effect of the composition for external application for skin according to the present invention, the degree of blood circulation in the skin was measured using LDPI (Laser Doppler Perfusion Imager). LDPI is a widely used and widely used device for measuring blood circulation in skin. It is a highly sensitive device that can measure not only blood velocity and quantity in the capillaries of the skin, but also flow in the small artery and the parenchyma.

The face was soaked in a constant temperature and humidity chamber, and then adapted for 30 minutes. Initial values were measured using LDPI. First, the initial blood flow in the lower part of the forehead of 30 women with cold hands and feet was measured by LDPI. The results of the blood flow measured after the preparation examples 1 and 2 and the comparative preparation examples 1 and 2 were used for the test subjects for 1 week were compared with the initial measured values (skin blood flow change) .

LDPI results before and after using cosmetics - skin blood flow Test substance Percent change of skin blood flow after 1 week use (%) Production Example 1 11 Production Example 2 14 Comparative Preparation Example 1 One Comparative Production Example 2 5

The results of Table 10 show that Production Examples 1 and 2 containing the germinated ginseng seed extract of the present invention significantly increase the skin blood flow rate compared with Comparative Production Example 2 containing the germinated ginseng seed extract which did not germinate, The improvement in color tone can be confirmed. This suggests that a composition containing germinated ginseng seed extract according to the present invention can contribute to effectively transmit nutrients of the skin and inhibit and delay skin aging.

[Test Example 11] Effect of suppressing sebum secretion

&Lt; 5a-reductase inhibitory effect &lt; RTI ID = 0.0 &gt;

In order to confirm 5α-reductase inhibitory effect, the ratio of [ ¹⁴ C] testosterone to [ ¹⁴ C] dihydrotestosterone (DHT) in HEK293-5αR2 cells was measured. HEK293 cells were transfected with p3 x FLAG-CMV-5αR2, and cultured in 2.5 × 10 ⁵ cells / well in a 24-well plate (Park et al., 2003, JDS Vol. 31, pp. 191-98). The next day, the medium was changed to a new medium supplemented with an enzyme substrate and an inhibitor. 0.05 [mu] Ci [ ¹⁴ C] testosterone (Amersham Pharmacia biotech, UK) was used as the substrate of the medium.

In order to confirm the degree of inhibition of 5α-reductase activity, Examples 1 and 2 of the germinated ginseng seed extract according to the present invention and a comparative example of ginseng seed extract not germinated were placed in a 37 ° C., 5% CO ₂ incubator for 2 hours Lt; / RTI &gt; At this time, no ginseng seed extract was used as a negative control, and finasteride was used as a positive control. Thereafter, the culture medium was collected, and the steroid was extracted with 800 쨉 l of ethyl acetate. The upper organic solvent layer was separated, dried, and the remaining residue was again dissolved in 50 쨉 l ethyl acetate to obtain a silica plastic sheet kiesel gel 60 F254 ) Using ethyl acetate-hexane (1: 1) as the solvent.

After drying the plastic sample in air, the bath system was used to measure the amount of isotope. The dried plastic sheet and x-ray film were put together in a bath cassette, and after 1 week, the testosterone remaining in the film and isotopes of dihydrotestosterone The conversion and inhibition rates were calculated according to the following equations (2) and (3), respectively, and the results are shown in Table 11 below.

Test substance Conversion Rate (%) Inhibition rate (%) Negative control group 48.0 - Positive control group 27.6 42.5 Example 1 32.2 32.9 Example 1 29.8 37.9 Comparative Example 41.1 14.4

In the results shown in the above Table 11, 5α-reductase, which converts testosterone into dihydrotestosterone and binds to the receptor protein in cytoplasm to enter the nucleus to activate sebaceous glands and promote differentiation, Activity of the ginseng seed extract of Examples 1 and 2 according to the present invention was inhibited more effectively than that of the non-germinated ginseng seed extract of Comparative Example, thereby blocking the conversion of testosterone to dihydrotestosterone. Therefore, it can be confirmed that the composition according to the present invention effectively inhibits the activity of 5? -Reddactase, thereby suppressing the hypersecretion of sebum.

&Lt; Effect of suppressing sebum secretion &

In order to examine the effect of the composition for external application for skin according to the present invention on sebum secretion suppression, the following evaluation was made. 30 men and women who felt that sebum secretion was high were selected and the nutritional creams of Preparation Examples 1 and 2 and Comparative Preparation Examples 1 and 2 were made every day for four weeks in designated areas. The results are shown in Table 12. The results are shown in Table 12 below. The results are shown in Table 12 below. The results are shown in Table 12 below. Respectively.

Test substance Sebum reduction rate (%) After 2 weeks After 4 weeks Production Example 1 33 24 Production Example 2 39 31 Comparative Preparation Example 1 3 One Comparative Production Example 2 11 7

The results of Table 12 show that Production Examples 1 and 2 containing the germinated ginseng seed extract of Examples 1 and 2 according to the present invention as an active ingredient are superior to those of Comparative Preparation Example 2 containing germinated general ginseng seed extract It can be seen that the secreted sebum can be effectively inhibited.

Test Example 12 Effect of LPS (Lipopolysaccharide) Induced Cyclooxygenase-2 (COX-2) on Biosynthesis

Human fibroblasts (their primary cultures) were cultured in a 12-well plate incubator at a concentration of 105, and then subjected to LPS treatment. Then, the germinated ginseng seed extract of Example 1, the germination ginseng seed extract of Example 2, Ginseng seed extract at 1 ppm and 50 ppm. On the second day of culture, cells were harvested and the amount of cyclooxygenase-2 (COX-2) produced using the Westren blot method was quantitated. The comparison with the value measured with a densitometer with the control group as 100 is shown in Table 13 below. The control group was an untreated group cultured without treatment of ginseng seed extract.

Test substance (concentration: ppm) COX-2 biosynthesis (%) Example 1 50 35 One 49 Example 2 50 28 One 41 Comparative Example 50 52 One 77 Control group - 100

As can be seen from the results of Table 13, the germination ginseng seed extract of Example 1 and the germination germination germination seed extract of Example 2 according to the present invention are superior to the non-germinated ginseng seed extract of Comparative Example in the amount of LPS Induced decrease in the biosynthesis of cyclooxygenase-2 in a concentration-dependent manner, thereby suppressing the inflammatory reaction, thereby exhibiting the effect of alleviating the skin trouble symptoms.

[Test Example 13] Lipogenesis inhibition test

3T3-L1 cells, a fibroblast cell line of mice, were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) (Dulbecco's modified eagles medium, GIBCO BRL, Life Technologies) Well plates in a culture plate at 1 x 05 cells / well. After 2 days, the cells were replaced with fresh DMEM (containing 10% FBS) medium and cultured for 2 days. Then, the cultured cells were again induced to differentiate into DMEM (containing 10% FBS) containing 1 μg / ml insulin, 0.5 mM IBMX and 0.25 μM dexamethasone, and compared with those of Examples 1 and 2 After two days of treatment, caffeine (positive control) was treated at a concentration of 50 μM, and the cells were exchanged with insulin-containing DMEM for 5 days. After 5 days, the cells were replaced with normal medium (DMEM, containing 10% FBS) and cultured while observing until the cells morphologically changed into adipocytes.

In order to evaluate the effect of the composition according to the present invention on the inhibition of fat accumulation in adipocytes, staining III (S4136, sigma-aldrich) was carried out using 3T3-L1 adipocytes differentiated as above. The adipocytes were fixed with 4% paraformaldehyde (pH 7.2) in phosphate buffer at room temperature, washed with PBS (phsphate buffered saline), stained with SIII and then photographed and compared visually. The negative control group was prepared by adding a test substance or a test substance to which no comparative substance was added, and treated with 50 μM caffeine as a positive control group. The level of inhibition of fat accumulation was graded by dividing the degree of staining by +++, ++, +, -. The results are shown in Table 14.

sample % Inhibition Negative control group +++ Positive control group + Example 1 + Example 2 ++ Comparative Example -

As shown in Table 14, the ginseng seed extract according to Examples 1 and 2 of the present invention has lipid synthesis inhibitory effect equal to or better than that of caffeine, which is a known lipid synthesis inhibitor, It was found that the lipid synthesis inhibitory effect is superior to that of the extract of the seeds. Therefore, lipid synthesis is inhibited, and sebum is reduced, so that occurrence of acne can be suppressed.

[Test Example 14] Test for improvement of acne and reduction of sebum secretion and stimulation

120 persons having acne were divided into three groups of 10 persons each, and the cosmetic composition prepared in Preparation Examples 1 to 2 and Comparative Preparation Examples 1 to 2 was used for one month to subjects belonging to each group. Acne improvement scale is from 1 point to 5 points, 1 point is 'No', 3 points are 'Normal' and 5 points are 'Very agree'. The experimental results are shown in Table 15 below with an average score of 10 persons.

The acne extermination period was based on the number of days of disappearance readings, and the results were based on the results after 1 month with or without recurrence of acne. The reduction of sebaceous secretion was from 1 point to 5 points, 1 point was 'No', 3 points were 'Normal' and 5 points were 'Very agree'. The experimental results are shown in Table 15 below with an average score of 10 persons. The presence or absence of skin irritation was evaluated as (number of stimuli) / (total number of test subjects).

Inflammatory acne improvement Acne-bloating period Recurrence of acne Decrease sebaceous glands Presence or absence of stimulation Production Example 1 3.8 3.6 1.6 3.4 1.7 Production Example 2 4.2 4.4 1.4 3.8 1.8 Comparative Preparation Example 1 1.4 1.2 4.2 1.5 1.2 Comparative Production Example 2 2.2 2.4 3.8 1.9 1.5

As shown in Table 15, the production examples 1 and 2 had no recurrence of acne as compared with the comparative preparative examples 1 and 2, and the acne improvement was excellent overall. In addition, Production Example 2 containing germanium ginseng seed extract according to the present invention is superior to Production Example 2 containing germinated ginseng seed extract in terms of acne improvement, sebum secretion and stimulation relaxation effect.

[Test Example 15] Antimicrobial effect on dandruff

Antibacterial effect of tea tree oil, which is widely used in anti-dandruff products as Examples 1 and 2, Comparative Example, zinc pyrithione, pylontonol amine and natural product, Were compared using the Minimum Inhibitory Concentration (MIC) assay.

The MIC measurement method is as follows. Each sample was diluted at a predetermined concentration interval in a liquid medium and cultured by inoculation with P. vorulatus ovalet, and then the degree of growth of the microbial cells at each sample concentration was observed to determine the minimum concentration for inhibiting microbial growth.

Ingredients Example 1 Example 2 Comparative Example Zinc pyrichion Protonolamine Tea trio day MIC (ppm) 800-1000 700 to 850 1300 ~ 1500 5 to 10 100 to 200 800-1000

According to the MIC measurement, the MICs of Examples 1 and 2 were lower than those of representative anti-dandruff materials such as zinc pyrithione or pyrrothonolamine. However, compared to the MIC of natural tea tree oil having a MIC of 1000 ppm, Respectively. In addition, the ginseng seed extract showed better anti-inflammatory effect than the ginseng seed extract, and thus it is expected to alleviate the symptoms of scalp troubles such as itch, Compared to synthetic antimicrobial zinc pyrithione and pylotonol amine, the antimicrobial activity was low, but antimicrobial activity of antimicrobial growth inhibitory effect similar to tea trioyl, which is currently applied to many products, is exhibited. Considering that the ginseng ginseng seed extract is a natural product and can be used safely because it is safe for human body, it will have sufficient utility value as anti-dandruff and anti-itch ingredients.

[Production Examples 3 and 4, Comparative Production Example 3]

Compositions containing the extracts of Examples 1 and 2 and Comparative Examples were prepared by mixing according to the conventional method with the composition shown in Table 17 below.

Component (% by weight) Production Example 3 Production Example 4 Comparative Production Example 3 Example 1 (germinated ginseng seed extract) 0.1 - - Example 2 (germination germination seed extract) - 0.1 - Comparative Example (Common ginseng seed extract) - - 0.1 Caprylic Capric Triglyceride 70 70 70 ethanol 10 10 10 water Balance Balance Balance

[Test Example 16] Test for preventing hair loss

In order to test the effect of preventing the hair loss of the composition according to the present invention, the experiment on the effect of preventing hair loss was carried out for sixteen weeks on the subjects having the initial hair loss phenomenon using the composition prepared in Table 17 above. The compositions of Preparation Examples 4 and 5 and Comparative Preparation Example 6 were used once a day for 16 weeks, and 16 weeks later, when the hair was wrapped again, decolorization was collected and counted. The results are shown in Table 18 .

Treated group Number of dropouts (average) / standard deviation Control group 82.80 / 10.32 Production Example 3 52.23 / 7.41 Production Example 4 44.71 / 6.85 Comparative Production Example 3 72.36 / 9.22

As shown in Table 18, when the preparations 3 and 4 containing the germinated ginseng seed extract were treated, the number of decay moths was significantly reduced as compared with Comparative Preparation Example 3 containing the ginseng seed extract not germinated .

[Test Example 17] Hair gloss enhancement effect

0.1 g of the hair sample was washed with 0.1 g of shampoo, washed thoroughly with flowing water, and then the hair sample was dried at 25 ° C and 50% humidity for 6 hours. Then, 0.1 g each of the compositions of Production Examples 3 and 4 and Comparative Production Example 3 of Table 17 were applied, and the gloss was measured using a cone photometer in the following manner.

The hair samples were measured for gloss in a cone photometer equipped with a halogen lamp. The cone photometer has a light incident angle of 30 degrees and can obtain a reflected value from 0 to 90 degrees. The gloss was calculated by the following equation.

The gloss is less diffuse and the more the specular reflection occurs, the better the result. The measurement results are shown in Table 19 below.

Treated group d Sf G Control group 6.49 68.41 10.54 Production Example 3 6.32 110.23 17.44 Production Example 4 6.29 137.52 21.86 Comparative Production Example 3 6.43 92.3 14.35

From the results shown in Table 19, it can be seen that the hair gloss was significantly improved when the preparations 3 and 4 containing the germinated ginseng seed extract were treated, as compared with Comparative Preparation Example 3. [

[Test Example 18] Confirmation of promotion effect of adipocyte differentiation

Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Lonza, Inc. (Walkersbille, MD, USA) and cultured according to Lonza's guidelines. Adipocyte differentiation was carried out using a method recommended by Ron, except that troglitazone (TRO) was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells. Specifically, mesenchymal stem cells are differentiated into adipocytes by inserting 1 g / ml of insulin, 1 M of dexamethasone, DEXA, 0.5 mM of isobutylmethylxanthine (IBX) and 2 M of TRO into the culture medium of mesenchymal stem cells . In the case of the experimental group, germinated ginseng seed extract of Example 1, germination germination seed extract of Example 2, common ginseng seed extract of comparative example, troglitazone, glibenclamide (Sigib-aldrich , USA) were each treated with 10M (treated at intervals of two days for 10 days), and 10M of each was added to the medium. After 10 days of induction of differentiation of mesenchymal stem cells into adipocytes, the cell culture medium was removed and the cells were washed with phosphate buffered saline (PBS). Cells were washed with 0.2 ml / cm ² of 10% formalin / PBS solution Was fixed for 10 minutes. The lipid bodies in a washed once fixed cells in 60% isopropanol solution 0.5ml / cm ^2, and oil red O (Oil Red O) for 10 minutes for dye dissolved in 60% isopropanol in 0.2ml / cm ² adipocytes Lt; / RTI &gt; The dyed oil red oi was eluted with a 100% isopropanol solution, and the absorbance was measured at a wavelength of 500 nm.

The results are shown in Table 20 below. Each data value was calibrated to an IDX negative control.

Treated group Oil Red Relative Dyeing Ratio Control group 1.00 Example 1 2.75 Example 2 3.12 Comparative Example 1.54

As shown in Table 20, ginseng ginseng seed extract was superior to non-ginseng ginseng seed extract in promotion of differentiation of adipocytes (P value <0.01).

When the composition for external application for skin according to the present invention is formulated in the form of a cosmetic composition, it may be formulated into cosmetic formulations such as softening lotion, convergent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, And the formulations thereof are not particularly limited. In addition, the composition according to the present invention can also be used as a lubricant, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

In addition, the composition according to the present invention may be a pharmaceutical composition. The pharmaceutical composition may further contain a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a pharmaceutical adjuvant such as a salt and / or a buffer for controlling osmotic pressure, and other therapeutically useful substances. The pharmaceutical composition may be formulated into lotions, creams, ointments or gels. The pharmaceutical composition is preferably transdermally administered.

The dosage of the germinated ginseng seed extract which is an effective ingredient of the pharmaceutical composition varies depending on the age, sex, and weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, will be. Dosage determinations based on these factors are within the level of those skilled in the art. Generally, the dosage ranges from 0.0001 mg / kg / day to about 2000 g / kg / day.

Formulation examples of the cosmetic composition and the pharmaceutical composition according to the present invention will be described below. However, the present invention can be applied to various formulations other than the following examples.

[Formulation Example 1] Flexible lotion (skin lotion)

A flexible lotion was prepared according to a conventional method according to the composition shown in Table 21 below.

ingredient Content (% by weight) Germination ginseng seed extract 1.0 glycerin 3.5 Oleyl alcohol 1.5 ethanol 5.5 Polysorbate 80 3.2 Carboxyl vinyl polymer 1.0 Butylene glycol 2.0 Propylene glycol 2.0 Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 2] Nutritional lotion (Milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 22 below.

ingredient Content (% by weight) Germination ginseng seed extract 1.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capriciculiglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 3] Nourishing cream

Nutritive creams were prepared according to the compositions shown in Table 23 below in a conventional manner.

ingredient Content (% by weight) Germination ginseng seed extract 1.0 glycerin 3.5 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capriciculiglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 4] Massage cream

Massage cream was prepared according to a conventional method according to the composition shown in Table 24 below.

ingredient Content (% by weight) Germination ginseng seed extract 1.0 glycerin 8.0 Butylene glycol 3.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 5] Pack

The packs were prepared in a conventional manner according to the composition shown in Table 25 below.

ingredient Content (% by weight) Germination ginseng seed extract 1.0 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative Suitable amount Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 6] Pharmaceutical preparation for local administration (patch)

The patches were prepared in a conventional manner according to the compositions shown in Table 26 below.

ingredient Content (% by weight) Germination ginseng seed extract 1.0 Beta-1,3-glucan 3.0 Diethylamine 0.7 Sodium sulphate 0.1 Polyoxyethylene lauryl ether (E.O. = 9) 1.0 Polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000) 1.0 Viscous paraffin oil 2.5 Caprylic acid ester / capric acid ester (Cetiol LC) 2.5 Polyethylene glycol 400 3.0 Polyacrylic acid (Carbopol 934P) 1.0 Purified water Balance system 100

Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

Claims (7)

A composition for external application for skin for improving the symptoms of scalp and hair trouble containing ginseng extract of ginseng as an active ingredient. 2. The composition for external application for skin according to claim 1, wherein the germinated ginseng seed extract is extracted by subcritical extraction. [3] The composition for external application for skin according to claim 1, wherein the ginseng seed extract is 1 to 50% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the trouble symptoms of the scalp and hair are hair loss, dandruff, or hair loss and dandruff. A composition for external application for skin for improving at least one of gloss and softness of hair containing ginseng extract of ginseng as an active ingredient. [Claim 7] The composition for external application for skin according to claim 5, wherein the ginseng seed extract germinated is extracted by subcritical extraction. [Claim 6] The composition for external application for skin according to claim 5, wherein the ginseng seed extract is 1 to 50% by weight based on the total weight of the composition.

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