KR20180051095A - Diet beverage comprising white grup and preparation method thereof - Google Patents

Abstract

The present invention relates to a diet beverage containing a cicada larva and a method for manufacturing the same. The present invention performs self-aggregation by surface coating a mixed extraction solution with chitosan, wherein the mixed extraction solution is manufactured with an old pumpkin extract, a Lycium chinense extract, a poria cocos extract, a psyllium seed extract, an angelica root extract, and a kudzu extract depending on a composition ratio based on the cicada larva as a main material. The present invention can remove a unique odor of the cicada larva; can be conveniently drunk; can remove cytotoxicity; can improve bioavailability; can provide excellent antimicrobial activity; can provide an excellent blood cholesterol and neutral lipid reducing effect; and can be applied to the diet beverage. According to an embodiment of the present invention, the present invention provides an excellent effect of reducing plasma neutral lipid, plasma cholesterol, neutral lipid of epididymis kidney tissue, neutral lipid of kidney tissue, and cholesterol in liver tissue through an efficiency experiment using a mouse. Provided is the diet beverage capable of improving or preventing diseases selected from a group including hypercholesterinemia, hyperlipidemia, a coronary artery disease which is a complication of the hypercholesterinemia and hyperlipidemia, angina, a carotid disease, cerebral stroke, cerebral arteriosclerosis, cholesterol gallstone, type 2 diabetes, lipodystrophy, and obesity and chronic inflammatory disorders.

Description

TECHNICAL FIELD The present invention relates to a diet drink containing slugs and a preparation method thereof,

The present invention relates to a diet drink containing slugs and a method of preparing the same, and more particularly, to a slimming diet containing slugs as a main ingredient, an old pumpkin extract, a Gugija extract, a Baekbokryeong extract, a tea extract, It is convenient to drink, to remove toxic odor peculiar to slugs by self-aggregation by surface coating with a mixed extract of chitosan combined with a certain amount, to have no cytotoxicity, to improve bioavailability, to have excellent antimicrobial activity The present invention also relates to a technology for application as a diet drink excellent in blood cholesterol and neutral lipid lowering effect.

Obesity is dangerous in itself, but it causes complications such as respiratory disorders, heart disease, arteriosclerosis and cancer, which are very important for management and treatment as modern dietary management. Currently, Korea is using sibutramine, an appetite suppressant, and orlistant, which is a fat absorption inhibitor, to raise blood pressure and accelerate heart rate, so it is important for people with related diseases. . Therefore, research on obesity control and diet using natural materials is needed for the development of agriculture industry and the possibility of global competitiveness as well as the possibility of development if the fusion technology of Korean traditional medicine is poor.

On the other hand, a slug (scientific name: Protaetia brevitarsis seulensis (Kolbe)) refers to a larva (grub worm) belonging to the white spotted bellflower, and its herbicidal properties are slightly colder, and taste is mainly weed, bad blood, bad blood, , White pimples (white in white pupil eyes), broken bones, broken or sprained, broken heart, and so on. It is a medicinal product introduced in Dongbok-bong (traditional medicine book), which is a traditional medicine of Korea. In 2014, it announced the improvement of the white spotted flower larva (slug) announced by Gyeonggi Province Agricultural Research Institute for three weeks against Rat As a result of liver function tests, 33.1% of active oxygen elimination component, 51.2% of total glutathione, and 36.0 ~ 44.1% of liver enzymes were decreased in liver damage, 23.4% of triglyceride, 18.4% of total cholesterol, , Arteriosclerosis index It was found to have 58.7% lower, respectively.

As described above, the patent related to the slugs includes a protein source derived from a flower fly larva (Japanese Patent Registration No. 10-1359507), a method for producing white spotted spotted larvae for food, a registered patent No. 10-1491771, a method for producing a white spotted spotted flower suspension And an anti-obesity composition containing the same, a composition for preventing and treating an inflammatory disease comprising as an active ingredient a white spotted flower of the Japanese Patent Registration No. 10-1382400, a registered patent No. 10-1474900, a method for producing a snack using slugs as a main ingredient , Patent No. 10-1616688, Method for producing functional food using slugs, Registration No. 10-1349136, Composition for prevention or treatment of fatty liver or hyperlipidemia comprising insect glicosaminoglycan as an active ingredient, 1622784 Diet energy bar using edible insect and method of manufacturing thereof, Patent No. 10-1493916 Method of edifying insect, the edible insect, A is being studied as a functional food and the like method of manufacturing a food dough for using.

On the other hand, chitosan is present as chitin in shrimp, crab and mushroom which are the bark of insects and crustaceans, and the chemical name is Poly (1 → 4) 2-amino-2-deoxy-β-D-Glucan. Chitin has a low effective utilization rate and is mainly used for various purposes such as water-soluble, fermentable, or other derivatives. The properties of chitosan are variously used as natural biomaterials because they are well known in the fields of pharmaceuticals, foods, cosmetics, and household goods, and have known antioxidant ability, antimicrobial activity, cholesterol control, lipid adsorption and transfer function.

The patent related to the fermented chitosan for use in medicines is described in Patent No. 10-1564668, and a method for preparing the fermented chitosan for immune enhancement, and its use and registration Patent No. 10-0735675 There is a manufacturing method for improving the effective utilization ratio of collagen by increasing the low molecular weight and hydrophilicity.

In recent years, a variety of health drinks that combine wellness, health, diet, and tradition have gained popularity, and competition to launch related products in the beverage and restaurant industry is fierce. Accordingly, many health products using slugs or chitosan have been developed and marketed. However, there is a problem that the unique smell of slugs can not be removed and the sensory preference is low and the consumer's desire is not satisfied. The bioavailability, antibacterial activity, The effect of cholesterol lowering is insufficient.

Therefore, there is a demand for a slug-containing diet drink excellent in bioavailability, antimicrobial activity, visceral fat and cholesterol-lowering effect, which can satisfy consumer's desire because of its unique smell removed from the slugs and excellent in sensory preference.

1. Korean Patent No. 10-1359507 2. Korean Patent No. 10-1491771 3. Korean Patent No. 10-1382400 4. Korean Patent No. 10-1564668 5. Korean Patent No. 10-1659927 6. Korean Patent No. 10-0735675 7. U.S. Patent No. 6,521,268

1. Rolls B. J. et al., Obes. Res. 6: 1-11, 1988 2. Hansen D.L. et al., Am. J. Clin. Nutr. 68: 1180-1186, 1998 3. SME Technology Innovation Development Project Challenge: Research Director Ryouji "Development of Netraceuticals peptide conjugated glucan natural immunity reinforcing material" (2001. 5 ~ 2002. 4) 4. SangBong Seo, "A Study on the Development of Novel Water-soluble Chitosan and Its Applications to Environment, Fine Chemicals and Medical Use" Tokyo Institute of Technology (2003) 5. Hyung-Min Kim, SangBong Seo et al., "Nitric oxide production by high molecular weight water-soluble chitosan via nuclear factor-kB activation" International Journal of Immunopharmacology 22 (2000) 923-933

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a method for removing slimy odor, which is convenient for drinking, cytotoxicity, bioavailability, Which has excellent antimicrobial activity and is excellent in blood cholesterol and neutral lipid lowering effect.

In order to achieve the above-mentioned object, the present invention provides a method for producing a safflower extract, comprising: 10 to 50% by weight of a slug extract, 5 to 30% by weight of an old pumpkin extract, 3 to 20% By weight to 30% by weight of the extract, 3 to 10% by weight of the extract and 5 to 20% by weight of the extract of Angelica gigas Nakai; And 0.5 to 10% by weight of chitosan based on the total weight of the mixed extract.

The diet drink containing the slugs may be selected from the group consisting of hypercholesterolemia, hyperlipidemia or a complication thereof consisting of coronary artery disease, angina pectoris, carotid artery disease, stroke, cerebral arterial sclerosis, cholesterol gallstone, type II diabetes, lipid disorders, obesity and chronic inflammatory disorders Improvement or prevention of a disease selected from the group may be possible.

The present invention also relates to a method for producing the extract of the present invention which comprises (a) 10 to 50% by weight of a slug extract, 5 to 30% by weight of an old pumpkin extract, 3 to 20% by weight of a gugija extract, 5 to 20% 3 to 10% by weight of the extract and 5 to 20% by weight of the extract of Angelica gigantosa L. extract to obtain a mixed extract; And (b) mixing 0.5 to 10% by weight of chitosan with respect to the total weight of the mixed extract to obtain a mixed solution.

The slug extract can be prepared by distilling a mixture of 6 to 15 times by weight of water with high-pressure sterilized and freeze-dried slugs at 80 to 150 ° C for 8 to 20 hours.

Each of the old pumpkin extract, Gugija extract, Baekbokryei extract, tea extract, chrysanthemum extract and Angelica gigantum extract was mixed with 25 to 50 times by weight of water to old pumpkin, gugija, To < RTI ID = 0.0 > 150 C < / RTI > for 8 to 20 hours.

The step (b) may be performed in a nitrogen atmosphere and at a temperature of 60 to 120 ° C with stirring at 3000 to 5000 RPM.

The manufacturing method may further include (c) mixing the mixture and agar to gel, and sterilizing the mixture.

According to the present invention, a mixed extract prepared by combining old pumpkin extract, Gugija extract, Baekbok-ri extract, Cha-eu extract, Angelica keiskei extract, aggregation to provide a diet drink which is excellent in eliminating the unpleasant smell unique to the slugs, is convenient for drinking, has no cytotoxicity, has improved bioavailability, is excellent in antimicrobial activity, and has a blood cholesterol and neutral lipid lowering effect .

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a transmission electron microscope (TEM) image and (b) a self-aggregated particle schematic diagram of (a) a slime-containing diet drink prepared from Example 1 of the present invention.

In the following, various aspects and various embodiments of the present invention will be described in more detail.

The present invention relates to a method for producing a roasted poultice, which comprises 10 to 50% by weight of a slug extract, 5 to 30% by weight of an old pumpkin extract, 3 to 20% by weight of a Gujija extract, 5 to 20% And 5 to 20% by weight of Angelica gigas Nakai extract; And 0.5 to 10% by weight of chitosan based on the total weight of the mixed extract.

Old pumpkin (Cucurbita spp.) Is low in calories, emits waste in the body, promotes diuretic action, is good for diet and skin beauty, and is widely used to remove postpartum and post-operative swelling.

Lycium chinense Miller has been widely used for sexual function improvement since its convergence, and its weakness is sweet (cold). There is no strength in the back and knee, weakness in the blood, weakness in the face, whitish hair, effective in sleeping when not sleeping, pharmacological action, non-specific immune enhancement, hematopoiesis, cholesterol lowering, anti- Blood glucose levels were reported. It is good for supplementing the energy of tiredness and fatigued body. It is good for Betaine to inhibit liver fat accumulation, to restore damaged liver, and also to have a lot of beta carotene and vitamin C.

Poria cocas wolf is taste and sweet, its quality is not cleared to one side, it acts on heart, spleen and lungs. It is mainly applied to various symptoms caused by weak spleen function and smooth metabolism of the human body due to its action of strengthening spleen function and promoting diuretic action, and has no particular adverse effect. And fungi belonging to the fungus of the sclerotium refers to. Depending on the characteristics and processing methods, the gyeongryeong is divided into red ginseng (red ginseng), ginseng (ginseng), and bokryeoppy (茯 笭 skin). From July to March of next year, it is collected from pine forests and used as medicinal material.

The tea plant (Plantaginis Semen) has the effect of removing heat with plantain seeds and eliminating the phlegm and improving the sight. The taste is sweet and the quality is cold. Ishim Jung Lim, Samgwaji Co., uses the leaves and seeds as medicinal materials in the name of blue, and the leaves are called "car" and "tea". Both leaves and seeds have the efficacy of diuretic, fever, germane and Jinhae. Seeds are effective for cystitis, urethritis, gonorrhea, diarrhea, hepatitis and hypertension. Leaves are used for the treatment of colds, coughs, bronchitis, sore throat, jaundice, hepatitis and hematuria as well as symptoms of poor urination. The leaves contain plantaginin and aucubin. Seeds absorb the water and contain a lot of swelling fiber, which helps the bowel movement.

칡 (Pueraria lobata (Willd.) Ohwi) is mainly extracted from the soil in early spring and late autumn, cut into rhizomes, and dried in the sun. Sungwi is the sweetness, the god, the quantity. It is nontoxic, and goes to the spleen, the stomach, the lungs and the bladder. It treats the symptoms such as fever headache, infinite, and anti-sickness due to the external appearance. It sweats, eliminates the burning caused by the digestion, makes the food taste, digest well, and eliminates the heat of the chest. Hypertension, brain disease, tinnitus, lower limb symptoms and improve the pomegranate pomegranate more than 600 times the amount of vegetable estrogen is a large amount of health is also effective in women's menopausal health is also reported.

Angelica gigas Nakai uses Angelica gigas Nakai roots in Korea. In late autumn, remove the head (蘆 head) and the root (鬚 根) and cut it into thin slices and knead it with alcohol or roast it with alcohol. The taste is sweet and very warm and the quality is warm. Angelica contains essential oil, mortar and vitamin B12. It is very good medicine for women such as menstruation relief, menopause, and menstrual flow, because it has excellent blood circulation and efficacy. Constipation due to blood vessels, such as constipation, stiff and dry constipation, geriatric constipation is good. If you wash your face with water, you can make your skin moisturized and acne effective.

The diet drink containing the slugs according to the present invention is advantageous in that the active ingredients of the natural herbal medicine described above can be efficiently accumulated by using chitosan so that it is not only convenient for drinking but also eliminates the unpleasant smell of slugs and has antibacterial activity and bioavailability .

(A) transmission electron microscope (TEM) images and (b) self-aggregated particle flow diagrams of the slimy diet drink prepared from Example 1 of the present invention in Fig. 1 .

Referring to FIG. 1 (a), it can be confirmed that the mixed extract is self-aggregated by the surface treatment of chitosan to exhibit a micelle shape.

1 (b) shows a micellar particle of FIG. 1 (a) in detail, wherein the four green particles represent the mixed extract according to the present invention, and the ocher color surrounding the four green particles is chitosan . As a result, it can be understood that the mixed extract is coated on the surface by chitosan. That is, it may mean that the extracted component, the weak component or the active ingredient of the mixed extract is self-aggregated by chitosan.

The diet drink containing the slugs may be selected from the group consisting of hypercholesterolemia, hyperlipidemia or a complication thereof consisting of coronary artery disease, angina pectoris, carotid artery disease, stroke, cerebral arterial sclerosis, cholesterol gallstone, type II diabetes, lipid disorders, obesity and chronic inflammatory disorders Improvement or prevention of a disease selected from the group may be possible. It is not limited to the diseases listed above.

If you take too much cholesterol and do not consume excess cholesterol from exercise, you may develop a disease caused by excessive cholesterol intake. The disorder caused by the excessive intake refers to a hypercholesterolemia or a disease caused by the abnormality in cholesterol control function and accumulating cholesterol in the body or blood vessels at an appropriate level or higher, and it is also referred to as a cholesterol metabolism disorder-related disorder.

The present invention also relates to a method for producing the extract of the present invention which comprises (a) 10 to 50% by weight of a slug extract, 5 to 30% by weight of an old pumpkin extract, 3 to 20% by weight of a gugija extract, 5 to 20% 3 to 10% by weight of the extract and 5 to 20% by weight of the extract of Angelica gigantosa L. extract to obtain a mixed extract; And (b) mixing 0.5 to 10% by weight of chitosan with respect to the total weight of the mixed extract to obtain a mixed solution.

The slug extract can be prepared by distilling a mixture of 6 to 15 times by weight of water with high-pressure sterilized and freeze-dried slugs at 80 to 150 ° C for 8 to 20 hours.

As a specific example, the slugs fasted for 2 to 5 days are heated at 90 to 160 ° C, preferably 110 to 130 ° C, 0.5 to 2.0 kgf / cm ^3, preferably 0.8 to 1.5 kgf / cm ³ and saturated steam conditions High pressure sterilization for 5 to 25 minutes, preferably 10 to 15 minutes, can prevent irreversible coagulation and denaturation of enzyme and tissue proteins.

The autoclaved slugs are frozen at -40 to -100 ° C, preferably -60 to -80 ° C for 8 to 30 hours, preferably 12 to 24 hours, and then frozen for 20 to 55 hours, preferably 24 to 48 hours Lyophilizing for a period of time; And

The lyophilized slugs are mixed with water in an amount of 8 to 12 times by weight, preferably 9 to 11 times by weight, at 110 to 120 ° C, preferably at 113 to 117 ° C for 10 to 14 hours, Followed by distillative extraction for 13 hours to prepare a slug extract.

Through such a step of producing the slug extract, it is possible to improve problems such as aversion and flavor in the food of slugs.

Each of the old pumpkin extract, Gugija extract, Baekbokryeong extract, tea extract, ginseng extract and ginseng extract is mixed with 25 to 50 times by weight water, preferably 30 to 50 times by weight, 40 times the weight of the mixture is heated at 80 to 150 DEG C, preferably 110 to 120 DEG C, more preferably 113 to 117 DEG C for 8 to 20 hours, preferably 10 to 14 hours, more preferably 11 to 13 hours For a period of time.

The number average molecular weight of the chitosan may be 100,000 to 250,000, preferably 13 to 180,000, more preferably 140,000 to 16, and the amount of chitosan to be mixed may preferably be 1 to 3%.

As a specific example of the production of the chitosan, stirring the mixture of a crust of an insect and an aqueous hydrochloric acid solution of 2 to 8% by weight at room temperature for 20 to 25 hours, preferably 22 to 23 hours, The mixture of the inorganic depleted mixture and the 1-6 wt% caustic soda aqueous solution is stirred and heated at 80 to 100 캜, preferably 85 to 90 캜, for 3 to 8 hours, preferably 5 to 6 hours, Removing; Deacetylating a mixture of the organic depleted mixture and a 40-50 wt% aqueous sodium hydroxide solution by heating; And chitosan having a number average molecular weight of 100,000 to 250,000 can be prepared through vacuum drying the solid shape obtained by filtering the deacetylated mixture.

The step (b) may be carried out in a nitrogen atmosphere at a temperature of 60 to 120 ° C, preferably 80 to 100 ° C, at a temperature of 3000 to 5000 RPM, preferably 3500 to 4000 RPM, To 30 minutes, preferably 10 to 20 minutes.

If the temperature range and the stirring speed range are out of the above-mentioned numerical range or not in the nitrogen atmosphere, the bioavailability, antimicrobial activity, weight loss effect, blood cholesterol and neutral lipid And the reduction effect was remarkably decreased.

The manufacturing method may further include (c) mixing the mixture and agar to gel, and sterilizing the mixture.

As a specific example, 0.5 to 5% by weight, preferably 1 to 3% by weight of the agar may be mixed with the mixed solution, and sterilization may be performed at 90 to 100 ° C for 3 to 10 minutes.

Hereinafter, production examples and embodiments according to the present invention will be described in detail with reference to the accompanying drawings.

Preparation Example 1: Preparation of slug extract

The slugs were fasted for 3 days, washed well and lyophilized. First to third instar screening of at least 3 g of the slugs after 20 hours and frozen at -70 ℃ freezer then autoclaved for 13 minutes, and the saturated water vapor in the autoclave 120 ℃ and 1.0 kgf / cm ³ under a condition, in the freeze-dryer 30 Lt; / RTI > Then 1,000 g of dried slugs were added to 10,000 mL of purified water and distilled at 115 ° C for 12 hours to prepare a slug extract.

Production Example 2: Preparation of mixed extract

A well-dried old pumpkin extract was prepared by adding 300 g of old pumpkin to 10,000 ml of purified water and distilling the mixture at 115 ° C for 12 hours.

Each of the extracts was prepared by extracting Gugija, Baekbokryeong, Chaejeon, Chung, and Angelicae in the same manner as above. The extracts of 30% by weight of the slug extract prepared in Preparation Example 1, 10% by weight of the old pumpkin extract, , 15% by weight of Baekbokryoung extract, 15% by weight of tea extract, 15% by weight of Angelica gigantosa extract, and 9% by weight of Lilium extract were mixed for 30 minutes to prepare a mixed extract.

Production Example 3: Preparation of chitosan

The collected insect carcass was stirred in 5% hydrochloric acid solution for 20 hours at room temperature to remove the minerals. The organic matter was removed by heating at 80 ° C for 5 hours under caustic soda 3% solution to obtain chitin. This was mixed with 45% Deacetylation reaction was performed under heating in a solution, and the solid shape filtered by filtration was vacuum-dried to prepare chitosan having a number average molecular weight of 150,000.

Example 1: Preparation of a diet drink containing slugs

3 wt% of chitosan was mixed with the total weight of the mixed extract prepared in Preparation Example 2, and the mixture was stirred at 4000 rpm under a nitrogen atmosphere and a temperature of 90 ° C. The mixed extract was surface-coated with chitosan to self- To prepare a diet drink.

Example 2

3% by weight of chitosan was mixed with stirring at 4000 rpm in a nitrogen atmosphere at 80 ° C with respect to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Example 3

3% by weight of chitosan was mixed with stirring at 4000 rpm in a nitrogen atmosphere at 100 ° C with respect to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Example 4

3% by weight of chitosan was mixed with stirring at 3500 rpm in a nitrogen atmosphere at 90 ° C to the total weight of the mixed extract solution prepared in Preparation Example 2 to prepare a diet drink.

Example 4

3% by weight of chitosan was mixed with stirring at 4500 rpm in a nitrogen atmosphere at 90 ° C to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Comparative Example 1

3% by weight of chitosan was mixed with stirring at 4000 rpm in air at room temperature to prepare a diet drink, in the same manner as in Example 1, except that the total weight of the mixed extract prepared in Preparation Example 2 was mixed.

Comparative Example 2

3% by weight of chitosan was mixed with stirring at 4000 rpm in air at 90 ° C with respect to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Comparative Example 3

3% by weight of chitosan was mixed with stirring at 4000 rpm in a nitrogen atmosphere at 40 ° C with respect to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Comparative Example 4

3% by weight of chitosan was mixed with stirring at 4000 rpm in a nitrogen atmosphere at 140 ° C to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Comparative Example 5

3% by weight of chitosan was mixed with stirring at 2500 rpm in a nitrogen atmosphere at 90 ° C with respect to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Comparative Example 6

3% by weight of chitosan was mixed with stirring at 5500 rpm in a nitrogen atmosphere at 90 ° C with respect to the total weight of the mixed extract prepared in Preparation Example 2 to prepare a diet drink.

Experimental Example 1: Cytotoxicity test

The cytotoxicity of the diet drinks prepared from the mixed extracts prepared in Preparation Example 2, Examples 1 to 5 and Comparative Examples 1 to 6 was measured by MTS assay using PC-12 cell line. As a positive control, sodium azide (NaN ₃ , sodium azide) was used for 48 hours at 37 ° C and 5% CO ₂ . Absorbance was measured at 490 nm by adding 0.2 ml of sodium azide to each of the above samples at 0.1, 0.05, 0.001 and 0.005% by weight, respectively.

As a result, it was confirmed that no cytotoxicity was observed at any of the concentrations of the mixed extract prepared in Preparation Example 2, Examples 1 to 5, and Comparative Examples 1 to 6.

Experimental Example 2: Bioavailability experiment

The dietary beverages prepared from the mixed extracts prepared in Preparation Example 2, Examples 1 to 5 and Comparative Examples 1 to 6 were prepared as 1 wt% solutions, and FITC, a fluorescent target material, The bioavailability after 48 hours in rat liver, blood and urine after injection into the tail vein of rats is shown in Table 1 below. Bioavailability was calculated as the percentage of FITC released at each time divided by the mass of injected FITC.

Referring to the following Table 1, only 68.1% of the mixed extract prepared in Preparation Example 2 was absorbed in the liver at 48 hours, and only 77.0% was excreted into the urine through blood. The samples of Examples 1 to 5 were absorbed in the liver by 84% or more at 48 hours, and were discharged into the urine through the blood of 95% or more. However, the sample of Comparative Example 1 in which chitosan was stirred by mixing was lower than that of Examples in the rate of discharging into urine for 48 hours. Comparative Example 2 in which chitosan was stirred in air, temperature range exceeding 60 to 120 占 폚 Of Comparative Examples 3 to 4 and Comparative Examples 5 to 6 mixed at a stirring speed range exceeding 3000 to 5000 RPM showed higher bioavailability than Comparative Example 1 but about 5 to 6% have.

division liver blood Urine Production Example 2 68.1% 79.2% 77.0% Example 1 84.4% 96.1% 95.2% Example 2 84.5% 96.0% 95.5% Example 3 84.2% 96.3% 95.2% Example 4 84.7% 96.5% 95.0% Example 5 84.3% 96.6% 95.1% Comparative Example 1 74.2% 84.3% 84.1% Comparative Example 2 80.5% 87.5% 88.2% Comparative Example 3 80.1% 87.1% 88.1% Comparative Example 4 79.5% 87.0% 88.4% Comparative Example 5 78.3% 87.3% 88.5% Comparative Example 6 79.2% 86.9% 88.1%

Experimental Example 3: Efficiency Experiment

As a test animal, ICR mice were used for dietary beverage prepared from the mixed extract prepared in Preparation Example 2, Examples 1 to 5 and Comparative Examples 1 to 6, Respectively.

In this experiment, the basic diet was based on AIN-76, the protein source was casein (Japan), the carbohydrate source was corn starch (Cheiljedang), and the fat source was corn oil (subject) . The dietary beverage used in this experiment was orally fed at a constant daily dose of 1 ml, and the diets and drinking water were freely taken. The animals were kept at constant temperature (20 ± 2 ℃), humidity (50 ± 5%) and maintained in a light condition for 12 hours. Two animals were kept in a polycarbonate cage. Cholesterol in the animal circulates along the bloodstream and is synthesized in several organs. When the cholesterol level in the bloodstream is high, it is a major cause of arteriosclerosis. The analysis items are (1) weight change and dietary intake, (2) blood cholesterol and neutral lipid .

After the breeding, the animal was fasted for 12 hours, then the ether was inhaled and the blood was collected from the abdominal subclavian vein. The plasma was separated by centrifugation (15 mins, 3,000 rpm) to measure the lipid content Respectively.

The internal organs (liver, epididymal, kidney) of each animal were immediately removed after blood sampling, rinsed several times with PBS (phosphate buffered saline) solution, and surface moisture was removed and compared. Plasma neutral lipids were measured by absorbance at 550 nm using the Asan kit for color measurement using enzymatic method of McGowan et al. (1983). Plasma total cholesterol was determined by the enzyme method of Allain et al. (1974) The asan kit was used to measure the absorbance at 500 nm. The absorbance was compared with the cholesterol standard curve. The lipid of the organs was cut by the method of Folch et al. (1957) ml, Chloroform: Methyl alcohol 2: 1, and neutral lipids and cholesterol were quantified in the same manner as in plasma. Each item was statistically treated as an average value.

(1) weight change

The body weight change and the dietary intake of the experimental animals are shown in Tables 2 and 3, respectively.

The results are shown in Table 2 below. In the case of the experimental animals fed orally with diet drinks prepared in Examples 1 to 5, the body weight was similar or decreased compared to that before the experiment, while the mixed extracts prepared in Preparation Example 2, The weight of the experimental animals and the normal group fed orally with the diet drinks prepared from the control group were increased compared to those before the experiment.

Referring to the following Table 3, it was confirmed that the dietary intake of the mixed extract prepared in Preparation Example 2, the dietary beverage prepared in Examples 1 to 5 and Comparative Examples 1 to 6, .

Although the dietary intake of all the experimental animals is similar, the weight of the experimental animals consuming the diet drinks of Examples 1 to 5 is not increased, and they are similar to those before the experiment, Respectively.

division Before the experiment (g) After the experiment (g) Production Example 2 27.01 29.95 Example 1 27.65 26.68 Example 2 27.51 27.05 Example 3 27.00 26.65 Example 4 27.11 26.98 Example 5 27.80 27.01 Comparative Example 1 27.75 28.32 Comparative Example 2 27.55 28.85 Comparative Example 3 27.45 28.48 Comparative Example 4 27.23 28.42 Comparative Example 5 26.89 28.06 Comparative Example 6 27.88 29.11 Normal group 27.85 30.04

division Test Control (g) Production Example 2 4.0 Example 1 3.9 Example 2 4.1 Example 3 4.3 Example 4 4.0 Example 5 3.9 Comparative Example 1 4.0 Comparative Example 2 4.1 Comparative Example 3 4.2 Comparative Example 4 4.2 Comparative Example 5 4.3 Comparative Example 6 4.2 Normal group 5.1

(2) Blood cholesterol and neutral lipids

Plasma neutral lipid, plasma cholesterol, epididymal neutral lipid, neutral lipid in kidney tissue and cholesterol in liver tissue of experimental animals are shown in Tables 4 to 8 below.

The results are shown in Table 4. Referring to Table 4, in the test control group in which the diet drinks of Examples 1 to 5 were consumed, the plasma neutral lipid was remarkably decreased compared to the experiment, while the mixed extract prepared in Preparation Example 2 and the diet drinks of Comparative Examples 1 to 6 There was no significant change in the intake of the test group, and the plasma neutral lipid level was increased in the normal group.

division Before the experiment (mg / dL) After the experiment (mg / dL) Production Example 2 121 119 Example 1 125 92 Example 2 126 95 Example 3 116 90 Example 4 126 101 Example 5 121 99 Comparative Example 1 121 120 Comparative Example 2 129 128 Comparative Example 3 110 118 Comparative Example 4 115 113 Comparative Example 5 118 118 Comparative Example 6 121 115 Normal group 124 136

Referring to the following Table 5, in the test control group in which the diet drinks of Examples 1 to 5 were consumed, the plasma cholesterol was remarkably decreased compared with that before the experiment, while the production group 2, the comparative examples 1 to 6 and the normal group showed no significant change You can see the sound.

division Before the experiment (mg / dL) After the experiment (mg / dL) Production Example 2 154 153 Example 1 156 122 Example 2 155 131 Example 3 149 130 Example 4 151 128 Example 5 152 118 Example 6 153 119 Example 7 159 121 Comparative Example 1 158 159 Comparative Example 2 157 156 Comparative Example 3 156 158 Comparative Example 4 150 148 Comparative Example 5 149 143 Comparative Example 6 151 148 Normal group 155 154

As shown in Table 6, the neutral lipids of the epididymis were markedly decreased in the test control group in which the diet drinks of Examples 1 to 5 were consumed, whereas the production of the diet drinks of Comparative Examples 1 to 6 was not significantly changed, In the normal group, it was confirmed that the neutral lipid of the hypothalamus increased.

division Before experiment (mg / g) After the experiment (mg / g) Production Example 2 24 23 Example 1 23 12 Example 2 28 18 Example 3 25 12 Example 4 26 10 Example 5 21 11 Comparative Example 1 22 21 Comparative Example 2 24 23 Comparative Example 3 25 26 Comparative Example 4 28 28 Comparative Example 5 26 30 Comparative Example 6 23 24 Normal group 24 34

Referring to Table 7, in the test control group in which the diet drinks of Examples 1 to 5 were consumed, the neutral lipid of the kidney tissue was remarkably decreased compared to that before the experiment, while the production of Example 2 and Comparative Examples 1 to 6 showed no significant change , And the normal group showed an increase in the neutral lipid of kidney tissue.

division Before the experiment (mg / dL) After the experiment (mg / dL) Production Example 2 3.2 3.1 Example 1 3.1 1.6 Example 2 3.0 1.7 Example 3 3.1 1.8 Example 4 3.0 1.6 Example 5 3.2 1.5 Comparative Example 1 3.2 2.8 Comparative Example 2 3.0 2.9 Comparative Example 3 3.1 3.2 Comparative Example 4 3.3 3.2 Comparative Example 5 3.0 3.0 Comparative Example 6 3.1 3.1 Normal group 3.4 3.8

Referring to Table 8, in the test control group in which the diet drinks of Examples 1 to 5 were consumed, the cholesterol of the liver tissue was remarkably decreased compared to that before the experiment, while the production group 2, the comparative examples 1 to 6 and the normal group showed significant changes .

division Before the experiment (mg / dL) After the experiment (mg / dL) Production Example 2 3.3 3.2 Example 1 3.3 2.1 Example 2 3.2 2.2 Example 3 3.1 2.0 Example 4 3.3 2.4 Example 5 3.5 2.4 Comparative Example 1 3.1 2.9 Comparative Example 2 3.5 3.4 Comparative Example 3 3.3 3.3 Comparative Example 4 3.6 3.5 Comparative Example 5 3.4 3.3 Comparative Example 6 3.0 2.8 Normal group 3.0 2.9

From the above results, it can be seen that the diet drinks manufactured through the examples according to the present invention can reduce the amount of the neutral lipids in the epididymal and kidney tissues, and thus can improve the visceral fat. In particular, the cholesterol And improvement of fat and cholesterol.

Experimental Example 4: Antibacterial activity test

The diet drinks prepared in Example 1 and Comparative Examples 1 to 6 were diluted with a 1 wt% solution to measure the minimum inhibitory concentration (MIC) required for inhibiting the growth of microorganisms against Streptococcus mutans and Escherichia coli ATCC 8739 Are shown in Table 9 below.

Referring to Table 9, it was confirmed that the MIX for Streptococcus mutans and E. coli was 1/5 to 1/6 that of Comparative Example 1 to 6, and that the antibacterial activity was remarkably excellent.

division Streptococcus mutans Escherichia coli ATCC8739 Example 1 25 μM 50 μM Comparative Example 1 100 [mu] M 250 μM Comparative Example 2 120 μM 300 μM Comparative Example 3 100 [mu] M 260 μM Comparative Example 4 125 μM 270 μM Comparative Example 5 115 μM 260 μM Comparative Example 6 118 μM 250 μM

Experimental Example 5: Sensory evaluation

The dietary drinks prepared from the mixed extracts prepared in Preparation Example 2, Examples 1 to 5 and Comparative Examples 1 to 6 were subjected to sensory evaluation.

20 panelists (10 males and 10 females) with more than three years of sensory experience in the food-related field tasted the diet drinks containing the slugs of Examples and Comparative Examples, followed by sensory evaluation, and the 9-point scale method 9). The results are shown in Table 10 below.

Referring to the following Table 10, the diet drinks containing slugs prepared in Examples 1 to 5 are superior to those of Preparation Example 2 and Comparative Examples 1 to 6 in overall flavor, overall taste, flavor, It can be confirmed that the unpleasant odor and taste specific to the slugs are significantly reduced.

- Overall incense, overall taste, hump, cusp and overall preference: 1 point = very bad, 9 points = very good

- Unpleasant smell and taste of slugs: 1 point = very weak, 9 points = very strong

division Production Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Overall incense 3.05 6.50 6.25 6.15 6.25 6.15 3.85 4.00 3.75 3.65 3.55 3.80 Overall flavor 3.20 6.45 6.05 6.20 6.20 6.10 4.05 3.80 3.85 3.60 3.50 3.75 End 1.80 6.55 6.45 6.50 6.55 6.40 3.50 3.55 3.30 3.35 1.95 2.85 cove 1.95 6.75 6.15 6.65 6.60 6.65 2.35 2.85 2.55 2.50 2.70 2.40 Unpleasant smell unique to slugs 7.85 1.05 1.35 1.10 1.15 1.20 6.25 5.85 6.70 6.55 6.85 6.15 Unpleasant taste of slugs 7.90 1.25 1.25 1.30 1.20 1.05 5.90 6.05 5.85 6.80 6.20 6.15 Comprehensive preference 1.98 6.45 6.30 6.25 6.30 6.25 2.05 2.35 2.10 2.15 2.05 2.20

Claims (7)

The extract of Liliaceae extracts in an amount of 5 to 30% by weight, the extract of Lilac extract in an amount of 3 to 10% by weight and the extract of Liliaceae in an amount of 10 to 50% by weight, an old pumpkin extract in an amount of 5 to 30% A mixed extract containing 5 to 20% by weight of Angelica gigas Nakai extract; And
And 0.5 to 10% by weight of chitosan based on the total weight of the mixed extract. The method according to claim 1,
Improvement of a disease selected from the group consisting of hypercholesterolemia, hyperlipidemia or a complication thereof selected from the group consisting of coronary artery disease, angina pectoris, carotid artery disease, stroke, cerebral arterial sclerosis, cholesterol gallstone, type II diabetes, lipid disorders, obesity and chronic inflammatory disorders A diet drink containing slugs characterized by being preventable. (a) 10 to 50% by weight of an extract of slugs, 5 to 30% by weight of an old pumpkin extract, 3 to 20% by weight of a ginger extract, 5 to 20% by weight of an extract of Baekbokryeong, 5 to 30% And 5 to 20% by weight of Angelica gigas Nakai extract to obtain a mixed extract; And
(b) mixing 0.5 to 10% by weight of chitosan with respect to the total weight of the mixed extract to obtain a mixed solution. The method of claim 3,
The slug extract is prepared by distilling a mixture obtained by mixing 6 to 15 times by weight of water with high pressure sterilized and freeze dried slugs by distillation extraction at 80 to 150 DEG C for 8 to 20 hours. ≪ / RTI > The method of claim 3,
Each of the old pumpkin extract, Gugija extract, Baekbokryei extract, tea extract, chrysanthemum extract and Angelica gigantum extract was mixed with 25 to 50 times by weight of water to old pumpkin, gugija, To 150 DEG C for 8 to 20 hours. The method of claim 3,
Wherein the step (b) is performed by stirring at 3000 to 5000 RPM under a nitrogen atmosphere and a temperature of 60 to 120 占 폚. The method of claim 3,
(c) mixing the mixture and agar to gel, and then sterilizing the slurry.

Images