KR20180013422A - Composition comprising Amla extract for preventing lose of hair or promoting of hair - Google Patents

Abstract

The present invention relates to a composition comprising an amla extract for preventing hair loss or promoting growth of hair. According to the present invention, a composition comprising an amla extract is safe not by showing cytotoxicity, has excellent antioxidant effects, increases enzyme activity helping in forming hair and expression of hair growth factors and promotes growth of hair in vivo, thereby having excellent effects of alleviating hair loss or promoting growth of hair and being used as a tea bag composition or a functional hair product for preventing hair loss or promoting growth of hair.

Description

[0001] The present invention relates to a composition for preventing hair loss or promoting hair growth comprising Amla extract,

The present invention relates to a composition for preventing hair loss or for promoting hair growth, and more particularly to a composition for preventing hair loss or promoting hair growth, which comprises an aqua extract.

As we move into modern society, patients with hair loss are increasing rapidly, and interest and anxieties about it are getting worse. Hair on the scalp of our body grows by 0.3mm every day, growing 15cm in a year, 85% of the total is growing, and the remaining 15% is said to stop growing. The missing hair is mainly hair that has stopped growing, which means that the percentage of hair that has stopped growing is increasing. In normal cases, the hair falls off 80 to 100 times a day, but if it does not, more than 100 are lost. When hair growth is shortened due to weakening of the hair cells and the growth period is extended, .

Hair baldness (毛毛 症, Baldness) refers to the hair of the body, especially the lack of hair. The most common cause of hair loss is known as genetic swelling, male hormone, and aging phenomenon. In addition, it is known that the hair loss is caused by the blood circulation disorder, excessive stress, nutritional imbalance, seborrheic dermatitis, Are also known to work in combination. In humans with genetic predisposition, testosterone is converted to 5-α dihydrotestosterone (DHT) by 5-α reductase, thereby delaying the protein synthesis of hair follicles and increasing the percentage of dormant hair follicles. do. The hair of the front part of the scalp and the hair of the two parts of the scalp are locally changed into a soft palate and become gradually thinner and shorter in length, and the hair follicle is miniaturized and disappears diffusely.

Treatment for hair loss includes oral dosing, topical application, hair grafting, and FDA approved medications Minoxidil and Finasteride. Minoxidil was developed as a vasodilator for the treatment of hypertension, but as a side effect, it was developed as a hair growth agent. Finasteride, which is used as an oral medicine, is used for the treatment of male pattern hair loss through a mechanism of lowering the concentration of dihydrotestosterone (DHT), an intermediate metabolite of androgen, by inhibiting the activity of 5-α reductase type II. However, the efficacy of Minoxidil and Finasteride is not uniform in every person, and side effects are reported. Currently, treatment for alopecia is not perfect, and the demand for effective and safe treatments is not decreasing. Therefore, there is a need for differentiated research through prevention of effective hair loss using herbal medicines and development of therapeutic substances.

Amla contains a variety of nutrients such as phyllembiln, gallic acid, zeatin and vitamin C. Particularly, natural vitamins such as 20 times of orange and 160 times of apple C.

The inventors of the present invention have made extensive efforts to overcome the problems of the prior arts. As a result, the inventors of the present invention have confirmed the excellent hair loss prevention or hair growth promoting effect and completed the present invention.

Accordingly, it is a main object of the present invention to provide a composition for preventing hair loss or for promoting hair growth, which comprises an extract of Amara which is excellent in preventing hair loss or promoting hair growth.

According to one aspect of the present invention, there is provided a hair loss preventing or hair growth promoting composition comprising an extract of Amaranth as an active ingredient.

As the number of patients with hair loss increased rapidly in the modern society, the treatment methods for them have also been diversified. However, the development of a complete method for treating alopecia is still insignificant. Accordingly, the inventors of the present invention have found that the extract of Amaranthus exudus is excellent in the prevention of hair loss and promotion of hair growth, while studying effective hair loss preventive and therapeutic substances using herbal medicines, and completed the present invention.

In the present invention, the extract can be extracted with any solvent conventionally used, and is preferably extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

In addition, the above extract can be extracted by any extraction method conventionally used, and preferably, Amara can be extracted by immersing in a solvent for 2 to 4 hours. As an extraction method using an extraction solvent, a conventional plant extraction method such as hot water extraction, reflux cooling extraction, ultrasonic extraction, supercritical extraction and the like can be used.

In the production method according to an embodiment of the present invention, the extract may be an ethanol extract, but is not limited thereto. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient in the medicinal product may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited and includes, for example, hot water extraction, cold extraction, ultrasonic extraction and reflux extraction. When the extract is concentrated, methods such as concentration under reduced pressure and reverse osmosis can be used. The post-concentration drying step includes, but is not limited to, spray drying, hot air drying, freeze drying, vacuum drying, vacuum drying, foam drying, high frequency drying,

In the present invention, the extract is concentrated and powdered to be used as a material for a functional tea bag.

In the present invention, the extract is used as a material for functional shampoos and treatments.

According to the experimental example of the present invention, in order to confirm the bioresistance of the aqua extract of the present invention, the in vivo test revealed that the aqua extract had an excellent effect on wool activity or a similar level as that of the positive control, The number of hair follicles was increased similarly to the positive control group. As a result of measuring the enzymatic activity to aid in hair formation, it was confirmed that enzyme activity was increased when Alaa extract was treated. In addition, it was confirmed that Alaa extract increased the expression of hair growth factor. Furthermore, it was confirmed that Amla extract is suitable as an antioxidant substance which has a great effect on decreasing DHT, which is the main cause of hair loss, by reducing the active oxygen which destroys proteins and helps circulation of the scalp. These results indicate that the extract of Amaranthus according to the present invention can exhibit the effect of preventing hair loss or accelerating hair growth (see Examples 1 to 6).

In addition, the aqua extract of the present invention does not show cytotoxicity and is safe as a tea bag material and a cosmetic material.

In the present invention, the extract is contained in an amount of 0.01 to 40% by weight based on the total weight of the composition. The weight range was set in consideration of the preference of each product and the stabilization of the product formulations when the product was developed from a functional tea bag product to a functional shampoo and a treatment product. In the case of shampoos and treatments, the formulation may not be stabilized when the weight range is exceeded, and the formulation may not be formed.

As described above, the composition containing the AQUA extract of the present invention is safe because it does not exhibit cytotoxicity, has excellent antioxidative effect, increases the activity of enzymes and hair growth factors that help in hair formation, So that it can be used as a tea bag composition for improving hair loss or promoting hair growth or as a functional hair product.

Fig. 1 is a diagram showing the results of measuring the antioxidative capacity (DPPH) of aqua extract.
FIG. 2 is a graph showing the results of measuring the antioxidative activity (SOD) of aqua extract.
Fig. 3 is a graph showing the measurement results of the dermal papilla cell proliferating ability of the aqua extract.
4 is a diagram showing a method of deriving a K value using Photoshop.
FIG. 5 is a graph showing the results of measurement of bovine ability in vivo (0 day).
6 is a graph showing the results of measurement of bovine ability in vivo (7 days).
Fig. 7 is a graph showing the results of measurement of bovine ability in vivo (10 days).
8 is a graph showing the results of measurement of bovine ability in vivo (14 days).
FIG. 9 is a graph showing the results of measurement of bovine ability in vivo (21 day).
Fig. 10 is a diagram showing the results of visual evaluation of bovine ability.
Fig. 11 is a diagram showing the results of evaluating the K value of bovine ability.
12 is a view showing the result of evaluating the bodily function in the tissue of the skin.
13 is a view showing the result of counting the number of hair follicles in the skin tissue.
14 is a graph showing the results of measurement of ALP enzyme activity.
15 is a graph showing the results of measurement of? - GT enzyme activity.
FIG. 16 is a graph showing the results of measuring changes in VEGF as a hair growth factor. FIG.
17 is a graph showing the results of measurement of IGF-1 change as a hair growth factor.
18 is a graph showing the results of measurement of changes in TGF-beta1 as a hair growth factor.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  One: Amla  Manufacture of water extracts

The cancer used in this experiment was imported from India and used dried raw material. 80 L of purified water was added to 10 kg of the un-pulverized raw material and then extracted at 95 캜 for 3 hours for the first time and 3 hours for the second time. It was then concentrated at 60 DEG C to 25 brix.

Example  2: Amla  Manufacture of alcohol extracts

Amaranthus sp. Extract was also imported from India. After adding 80L of alcohol to 10kg of raw material that was not ground, it was extracted at 95 ℃ for 3 hours for 1st and 2nd for 3 hours. It was then concentrated at 60 DEG C to 25 brix.

Experimental Example  One: Antioxidant ability  Measure

DPPH (2,2-diphenyl-1-pycryl-hydrazyl) and Superoxide were measured to evaluate the antioxidative activity of the extracts.

DPPH (2,2-diphenyl-1-pycryl-hydrazyl) is a very stable, water-soluble free radical, a violet compound that exhibits characteristic absorption at 520 nm. When it meets with antioxidants, the radical is cleared and DPPH's unique index, purple, The color difference was quantified by colorimetric determination of radical scavenging ability. Specifically, a DPPH solution was prepared by dissolving DPPH in MeOH, and then the sample was subjected to freeze-drying for 24 hours using a freeze dryer (Ilshin BioBase, FDS850) , And the yield was shown in Table 1.) was mixed with 1: 1, followed by shading and incubation at room temperature for 30 minutes. Then, the absorbance at 520 nm was measured. As a positive control, 50 μM ascorbic acid was used. The results were calculated by the following formula 1, and the results are shown in FIG.

Material name Solution before freeze-drying (mL) After freeze-drying the powder (g) yield(%) Amla 300 61.6 19.3 Amra Spirits 300 47.4 15.8

[Formula 1]

As a result, as shown in FIG. 1, the antioxidative ability of the extract of Amaranthus according to the present invention showed a concentration-dependent effect. The antioxidative effect of vitamin C was similar to that of the positive control group, Vitamin C, at 500 ㎍ / mL (Amla.; 72 ± 2.6%, Amra alcohol; 75.3 ± 0.5% Amaranthus sp. Extract showed a high antioxidative effect of about 60% at a low concentration of 10 μg / mL to 66 ± 1.5%.

In addition, Superoxide was measured for superoxide dismutase (SOD) activity using the principle that the oxidation rate of pyrogallol, which catalyzes the conversion of superoxide into H ₂ O ₂ in the presence of superoxide, is low. For the test, SOD assay kit (Dojindo Molecular Technologies, Rockville, USA) was used. Absorbance was measured by ELISA reader. Specifically, the samples were diluted to a concentration of 10 μg / mL, 50 μg / mL, 100 μg / mL, and 500 μg / mL, and 20 μL of the diluted samples were dispensed into a 96-well plate. 20 [mu] l of the working solution was incubated at 37 [deg.] C for 20 minutes and the absorbance was measured at 450 nm. As a positive control, 500 μg / mL trolox was used. The resultant value was calculated by the following formula 2, and the result is shown in FIG.

[Formula 2]

As a result, as shown in FIG. 2, the antioxidant ability of the extract of Amlazoa according to the present invention increased in a concentration-dependent manner and showed a significant difference from the negative control group. The highest antioxidant activity was observed in the 100 μg / mL Amlautral (64.9 ± 0.3), Amla (65.5 ± 0.6), and the positive control, 500 μg / mL trolox (33.7 ± 0.7% Antioxidant effect.

Experimental Example  2: Dairy cattle Cell proliferation ability  Confirm

Human dermal papilla cells (HFDPC) were used. Specifically, the cells were cultured in 96-well plates at a cell number of 1 × 10 ⁴ for 24 hours. After culturing the 96-well plate for 1 day by adding a medium containing a diluted compound, MTT reagent was used to measure cell proliferation ability. The results are shown in Fig.

As shown in FIG. 3, minoxidil, amaranth extract and alcohol extract used as a positive control group showed significant differences in cell proliferating ability at all concentrations except 500 μg / mL, as shown in the MTT assay. Ampicillin, and 500 ㎍ / mL of alcohol extract showed cytotoxicity. Cell proliferation was increased by 1.3-fold in the positive control group, minoxidil 1 μg / mL, as well as in 1 μg / mL of minoxidil at 100 μg / mL of Amaranthis extract and alcohol extract.

Experimental Example  3: C57BL / 6J Mouse Growth Follicle Induction Evaluation

The dorsal hair of a 6-week-old mouse with a period of resting period of hair growth was excised with an animalclipper (Panasonic ER1431p, Japan) and the hair removed with a hair removal cream bit (veet, Reckitt Benckiser, France). The test drug was applied for 3 weeks from the third day after the second epilation.

Specifically, application of the test drug was carried out after the mouse was anesthetized with an ether and fixed with a tape on a test stand. The mice were kept stationary for 5 minutes after application to avoid licking the test material. For the gross evaluation of wool, ethyl ether was anesthetized on the days 0, 7, 10, 14, 21, and the photographs were taken between the individuals and groups. The criteria were 0-19% 39% (+/-), 40-59% (+), 60-79% (++), 0-100% (+++). In order to obtain the numerical results for wool, the K value representing the black color of the image was derived using Photoshop program. The K value was converted into data by placing the back part of the mouse on the scale line set by using Photoshop (FIG. 4). The results are shown in Fig. 5 to Fig.

In order to quantify the data on wool, a total of 7 people participated and evaluated the degree of wool as 0 ~ 4 points. On day 21, all of the test drug groups showed a higher wool effect than the negative control group, and in particular, it showed a similar wool effect to minoxidil used as a positive control group in the Amaranth solution application group (see FIGS. 5 to 10).

In addition, the degree of darkness of wool (darkness / black) and the value of K were significantly increased compared with the negative control group at 7 days (19.2 ± 3.4). On the 10th day, all of the test drugs showed a significant increase compared to the negative control (8.9 ± 1.3), but in particular Amla (52 ± 4.8) showed similar bioresistance to minoxidil (50.3 ± 4.6) of Sigma. On the 14th day, Sigma minoxidil (73.8 ± 2.4) and Amara (76.3 ± 1.6) showed similar bioresistance. On the 21st day after the end of the experiment, the Amarant (75.3 ± 1.1) and Amara (78.1 ± 1.1) It was confirmed that it exhibited similar bioresistance to sigma minoxidil (74.1 ± 1.3) (see FIG. 11).

Experimental Example  4: Histological observation

After 10 and 21 days from the start of the animal test, fixed skin tissue was stained with hematoxylinandeosin (H & E), and the number of hair follicles per 5 mm ² area was counted by an optical microscope at a magnification of 100, and the results are shown in FIG. 12 and FIG.

The number of hair follicles in the skin tissue was found to increase with time in all test drugs. Also, at 21 days, the number of tissue follicles of all test drugs showed a significant difference when compared to negative control (42 ± 11). Sigma minoxidil tissue hair follicles (120.4 ± 15.5) were twice as many as those of modern medicine minoxidil (76.2 ± 8.4) and Amla (90.5 ± 11.3).

Experimental Example  5: Measurement of enzymatic activity of skin tissue

The skin tissue was cut and made into micro slices under ice-cooling. A certain amount of the slices were weighed, and then homogenized with a homogenizer by adding phosphate buffer solution (PBS) four times the skin tissue. After that, the homogenate was centrifuged at 12,000 rpm for 20 minutes using a centrifuge at 4 ° C, and the supernatant was analyzed for alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (γ-GT) using an automatic biochemical analyzer. The results are shown in Figs. 14 and 15.

ALP enzyme is known as an angiogenesis index of hair growth. It induces wool function by nutrition by capillary blood vessels. ALP enzymes show high activity during hair growth period and decrease significantly at rest period. The ALP enzyme in the skin tissue was measured and compared with the negative control group (33 ± 13 U / L) 10 days after the test substance application, the positive control group modern drugs minoxidil (155.25 ± 32.84 U / L), sigma minoxidil (156.8 ± 7.6 U / L) shows a significant increase. The test drug showed a significant increase from the control group (119.67 ± 46.6 U / L) compared to the negative control group. After 21 days of the test substance application, the negative control (132.33 ± 4.16 U / L), respectively.

γGT is involved in the in vivo oxidative stress, glutathione metabolism, and the absorption and secretion of amino acids and peptides through the cell membrane, and is highly expressed in proliferating and dividing cells, exhibiting high activity during hair growth, and greatly decreasing in resting period . As a result, the positive control group, minoxidil (37.25 ± 13.86 U / L) and sigma minoxidil (39.67 ± 9.87 U / L), compared to the negative control group (2.75 ± 1.25 U / L) (12.5 ± 2.12 U / L) compared to the negative control group.

(23.6 ± 5.5 U / L) and minoxidil (24.67 ± 5.03 U / L) compared to the negative control group (21.3 ± 2.88 U / L)

Experimental Example  6: Measurement of hair growth factor in skin tissue

Vascular endothelial growth factor (VEGF), insulin like growth factor (IGF) -1 and transforming growth factor beta 1 (TGF-β1) were measured. Specifically, the mouse skin tissue was extracted with the eszy-Blue ™ Total RNA extraction kit (iNtRON Biotechnology, Korea), and then extracted with TaqMan RNA-to-Ct ™ 1-Step Kit IGF-1), transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF) were mixed and incubated for 15 min at 48 ° C, 1 min at 95 ° C for 15 min, And the threshold cycle value was measured by repeating 40 times for 1 minute at 20 DEG C. The results are shown in FIGS.

Vascular endothelial growth factor (VEGF) is a typical wool-related factor by promoting neovascularization in the dormant period and inducing new hair growth period to participate in scalp and hair cell division. The expression of VEGF mRNA in the dermal tissues was significantly increased in Sigma minoxidil (2.57 ± 0.15) and Amara (4.5 ± 0.69) when compared to the negative control group (1.00 ± 0.06) (See Fig. 16).

In addition, insulin like growth factor (IGF) -1, a hair growth factor present in the skin, promotes functional activity of the hair to inhibit hair cell death. On day 21, IGF-1 mRNA in the dermal tissue was measured and no significant change between the groups was observed (see FIG. 17).

Transforming growth factor beta 1 (TGF-β1) is a factor that interferes with hair growth in the period of hair growth. As a result of measurement of TGF-β1 mRNA in the skin tissue on the 21st day, no significant change between the groups was observed (see FIG. 18).

In conclusion, the Amaranth gum of the present invention showed a high antioxidative effect and significantly increased the growth rate of the dermal papilla cells. In addition, wool activity was significantly higher than that of 3% Sigma minoxidil, which was used as a positive control in gross and histological evaluation (hair follicle number) of Amaranthus spp. These bioredeses are thought to shorten the time for hair regrowth in the hair growth cycle by ALP, γ-GT, and VEGF involved in the angiogenesis in the skin tissue, which affect hair growth in C57BL / 6J.

Claims (5)

A composition for preventing hair loss or promoting hair growth comprising an extract of Amla as an active ingredient.
The composition for promoting hair loss prevention or hair growth according to claim 1, wherein the aqua extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
[2] The composition for promoting hair loss prevention or hair growth according to claim 1, wherein the extract is concentrated and powdered to be used as a material for a functional tea bag.
The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the extract is used as a material for functional shampoos and treatments.
[5] The composition for promoting hair loss prevention or hair growth according to claim 4, wherein the extract is contained in an amount of 0.01 to 30% by weight based on the total weight of the composition.

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