KR101615314B1 - Composition for prevention of hair loss and promotion of hair growth containing extract of feremented herbal medicine - Google Patents
Composition for prevention of hair loss and promotion of hair growth containing extract of feremented herbal medicine Download PDFInfo
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- KR101615314B1 KR101615314B1 KR1020150076068A KR20150076068A KR101615314B1 KR 101615314 B1 KR101615314 B1 KR 101615314B1 KR 1020150076068 A KR1020150076068 A KR 1020150076068A KR 20150076068 A KR20150076068 A KR 20150076068A KR 101615314 B1 KR101615314 B1 KR 101615314B1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
Abstract
Description
The present invention relates to a composition for preventing hair loss and promoting hair growth comprising a fermented extract of a medicinal substance.
Hair is a vital part of the body that is a measure of health and determines the appearance, although it is not an important organ for life maintenance [1]. Hair loss has been recognized as a series of aging phenomena, but it has recently been revealed that hair loss is caused by various causes, such as stress, westernized eating habits, nutritional imbalance, and changes in social activities.
The hair is produced in hair follicles, a structure in which the skin is embedded by continuous growth of stromal cells in the base of the scalp, and has a hair cycle including various growth stages. Hair consists of anagen, growing phase, 90% of normal hair, catagen, transitional phase with growth arrest and hair growth, telogen, resting phase, growth and hair loss are repeated according to the four stages of the growth cycle.
The cause of hair loss is genetic factors, stress and aging. The representative mechanism is the conversion of male hormone testosterone (T) to dihydrotestosterone (DHT) by 5α-reductase (RD) . At age, DHT is increased and the protein synthesis of hair follicle cells is delayed, resulting in an increase in the percentage of dormant hair follicles, which leads to a rapid progression of hair loss.
Formation of hair has a cyclically cycling pattern. The anagen and gowing phases, which account for 90% of normal hair, the catagen and transitional phase, the telogen, resting phase, the hair grows and hairlessly undergoes four stages of hair cycle, including exogenous hair growth. In particular, during the retrogression, many hair follicles undergo apoptosis and enter the dormant period, thereby reducing the size of the hair follicles.
Minoxidil coating agent is mainly used as a therapeutic agent for alopecia, which is used as a main treatment for alopecia of male in addition to oral finasteride agent. However, these drugs should be used for a long period of time, and interest in natural products is increasing recently due to accompanying side effects such as cardiovascular disorders, skin irritation, decreased sexual function, malformation, itching, erythema, epidermal detachment and dry dermatitis.
Therefore, in order to comply with these shortcomings of existing products and to respond to the demand for products that are safe and effective for continuous use, researches on natural products including oriental herb medicine and herbal medicines, which are traditionally known to be effective for hair loss prevention and hair growth, It is progressing.
For example, Korean Patent No. 10-1498201 discloses a composition for preventing hair loss or improving hair growth comprising an extract of Aspergillus oryzae as an active ingredient. Korean Patent No. 10-1484033 also discloses a composition for preventing hair loss or hair growth comprising peanut bark extract The present invention relates to a composition for accelerating a reaction.
The inventors of the present invention completed the present invention while studying a natural material having an effect of preventing hair loss and promoting hair growth more remarkably.
It is an object of the present invention to provide a composition for preventing hair loss and promoting hair growth.
In order to achieve the above object,
The present invention
A composition for promoting hair loss prevention and hair growth, comprising a fermentation product of a medicinal composition, wherein the medicament comprises a herbal composition.
Also,
A method for preparing a composition for preventing hair loss and promoting hair growth comprising the steps of: preparing a medicinal mixture; and fermenting the medicinal mixture by inoculating the microorganism.
The composition of the present invention is effective in preventing hair loss and promoting hair growth.
Figure 1 shows the compositional change of the complex fermentation product according to the fermentation time.
FIG. 2 shows the hair growth effect with time after applying the samples to the hairless mouse, respectively.
Figure 3 shows changes in ALP and gamma-GT activity of mouse skin. Values represent the mean ± standard deviation of 5 mice. The test materials were applied to C57BL / 6 mice and the like for 3 weeks. Control group: saline, S-1: complex extract, S-2: complex fermented product, minoxidil: 5% minoxidil.
Values with different superscripts (a, b, c, d) are significantly different in ANOVA and Duncan's multiple range tests (p <0.05).
Figure 4 shows the effect of complex extract and complex fermented product on the expression of IGF-1. The test results were obtained using real-time PCR.
Results were expressed as mean ± standard deviation (n = 5). Values with different superscripts are significantly different in ANOVA and Duncan's multiple range tests (p <0.05).
FIG. 5 shows the effect of the combined extract and complex fermented product on the expression of VEGF (vascular endothelial growth factor). The test results were obtained using real-time PCR. Results were expressed as mean ± standard deviation (n = 5). Values with different superscripts are significantly different in ANOVA and Duncan's multiple range tests (p <0.05).
FIG. 6 shows the effect of complex extract and complex fermented product on the expression of TGF-? 1. The test results were obtained using real-time PCR. Results represent the mean ± standard deviation of 5 mice. Control group: saline, S-1: complex extract, S-2: complex fermented product, minoxidil: 5% minoxidil.
The present invention
A fermentation product of the pharmaceutical composition,
The medicament is for a composition for preventing hair loss and for promoting hair growth, which comprises a perennial herb.
Also,
Preparing a pharmaceutical mixture; and
Inoculating the medicament mixture with a microorganism and fermenting the medicament mixture,
And to a method for producing a composition for promoting hair growth.
Hereinafter, the present invention will be described in detail.
Medicinal
The medicinal product of the present invention includes a rhizome. The medicinal composition of the present invention may further comprise at least one selected from the group consisting of the outer perennial plant, the superficial root, the perennial plant, the mint leaf, the perennial plant, the perilla leaf, the camellia seed, the ginseng seed, the green tea and the licorice. Preferably, the medicament of the present invention is a mixture of Rhizobium, Rhizobia, Rhizobia, Phloxobacterium, Mint Leaf, Mature Rhizobia, Lyophyllum, Camellia seed, Ginseng seed, Green tea and Licorice. The medicinal materials of the present invention may be obtained by purchasing commercially available medicinal materials, or may be directly harvested or cultivated.
Medicinal mixture
The medicinal mixture of the present invention is a mixture of plural medicinal materials, including Houttuynia cordata. For example, the medicinal mixture of the present invention is a mixture of Rhizobium, Rhizobia, Rhizobia, Phloxobacterium, Mint Leaf, Mature Rhizobia, Lyophyllum, Camelliaceae, Ginseng Seed, Green Tea and Licorice. Preferably, the pharmaceutical composition of the present invention comprises 50 to 200 parts by weight of the top leaf, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the perianth, 50 to 200 parts by weight of the peppermint leaf, 50 to 200 parts by weight of the peppermint, 50 to 200 parts by weight of hemp leaf, 50 to 200 parts by weight of camellia, 50 to 200 parts by weight of ginseng seed, 50 to 200 parts by weight of green tea, and 50 to 200 parts by weight of licorice.
Medicinal Fermentation product
The fermented product of the medicinal mixture of the present invention is prepared by inoculating a strain into a pharmaceutical mixture and fermenting it and extracting it. At this time, it is preferable to sterilize the medicinal substance mixture and inoculate the strain into the sterilized medicinal substance mixture. At this time, water may be added to the pharmaceutical mixture before or after sterilization. The water may be added at 5 to 15 times the weight of the pharmaceutical preparation.
The strain is preferably a lactic acid bacterium, more preferably a lactobacillus, still more preferably a mixture of strains of the genus Lactobacillus, i.e., a complex strain. Most preferably, the strain is a mixture of strains selected from the group consisting of Lactobacillus adicopilus, Lactobacillus brevis, and Lactobacillus plantarum, i.e. a composite strain.
The fermentation is carried out by inoculating the herbicide mixture with the strain and fermenting at 25 to 35 DEG C for 2 to 10 days. The fermentation conditions are not particularly limited and may be any ordinary conditions.
The extraction is carried out by applying heat to the fermentation product of the pharmaceutical mixture. At this time, when the water content of the fermented product of the medicinal mixture is too low, water may be added to extract hot water.
The present invention reduces the side effects and improves hair loss prevention and hair growth promoting effects by fermenting and extracting the pharmaceutical mixture without performing simple extraction, for example, simply hydrothermal extraction.
Composition for preventing hair loss and for promoting hair growth
The composition of the present invention is useful for preventing hair loss, alleviating hair loss, improving hair loss, treating hair loss, promoting hair growth, improving scalp blood circulation, improving scalp hygiene, alleviating inflammation of scalp, relieving skin irritation of scalp, It has the effect of increasing density or increasing hair thickness.
The composition of the present invention is a cosmetic composition, a food composition or a pharmaceutical composition.
The cosmetic composition of the present invention can be prepared in any formulations conventionally manufactured in the art and can be used as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant- , Shampoos, sprays, and the like, but is not limited thereto. Preferably, the cosmetic composition of the present invention includes all types of cosmetic compositions used for hair and scalp. For example, the cosmetic composition of the present invention may be in the form of a hair cosmetic composition such as a shampoo, a hair conditioner, a hair gel, a hair lotion, a hair pack, a hair essence, a hair nutrient, It may be in the form of cosmetics for use.
Meanwhile, the present invention provides a cosmetic method for preventing hair loss and promoting hair growth, which comprises treating the scalp with a hair loss preventing and hair growth promoting composition according to the present invention. In this case, The compositions of the invention may be applied to the scalp. At this time, the composition of the present invention can be appropriately selected by those skilled in the art, and the specific treatment method of treating the scalp is not limited thereto.
The pharmaceutical composition of the present invention may contain 0.01 to 99 parts by weight, preferably 0.02 to 60 parts by weight, of the composition for preventing hair loss and hair growth of the present invention, based on 100 parts by weight of the pharmaceutical composition, Preferably 0.02 to 30 parts by weight. However, this can be increased or decreased according to the needs of the practitioner, and can be appropriately increased or decreased according to the condition of the scalp, the presence of inflammation, and the progress of hair loss.
The pharmaceutical composition of the present invention can be administered orally or parenterally, but is preferably applied to the skin and administered for transdermal absorption. The pharmaceutical composition of the present invention can be used in the form of a general pharmaceutical preparation or a milky lotion for skin application. The pharmaceutical compositions of the present invention can be prepared using conventional carriers acceptable for pharmaceutical or cosmetic use. For example, in the case of a preparation for oral administration, it can be prepared using excipients, binders, disintegrants, lubricants, solubilizers, suspending agents, preservatives or extenders.
The dose of the pharmaceutical composition of the present invention may be determined by a specialist depending on various factors such as the skin condition of the patient and age, but is generally 0.01 μg to 200 μg, preferably 0.01 μg to 50 μg ≪ / RTI > Also, the daily dosage of the pharmaceutical composition or a half, 1/3 or 1/4 dose thereof is contained, and it can be administered 1 to 6 times a day. However, in the case of long-term administration for the purpose of skin health maintenance and hygiene, the amount may be less than the above range, and since the active ingredient has no problem in terms of safety, it may be used in an amount exceeding the above range.
The food of the present invention may be a health supplement food, a health functional food, a functional food, and the like, but is not limited thereto, and it is also possible to add a composition for preventing hair loss and promoting hair growth of the present invention to natural food, processed food, do.
The food composition of the present invention can be used as it is as an active ingredient, or can be used in combination with other food or food composition, and can be suitably used according to a conventional method, as a composition for promoting hair loss prevention and hair growth of the present invention. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, improvement, or therapeutic treatment). In general, the composition for preventing hair loss and hair growth of the present invention may be added in an amount of 0.1 to 70 parts by weight, preferably 2 to 50 parts by weight, based on 100 parts by weight of the raw material for food or beverage, . The effective dose of the hair loss prevention and hair growth promoting composition may be used in accordance with the effective dose of the pharmaceutical composition but may be less than the above range for the purpose of health and hygiene or for long- , And the active ingredient can be used in an amount in the above range because there is no problem in terms of safety.
There is no particular limitation on the kind of the food. The food composition may be used in the form of tablets, hard or soft capsules, liquids, suspensions, and the like, which may contain conventional excipients, such as excipients in the case of oral preparations, Binders, disintegrators, lubricants, solubilizers, suspending agents, preservatives or extenders.
Examples of the foods to which the hair loss prevention and hair growth promoting composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen and other noodles, gums, ice cream, Beverages, tea, drinks, alcoholic beverages and vitamin complexes, and other nutrients, but are not limited to these kinds of foods.
Preparation phase of the medicinal mixture
The step of preparing the pharmaceutical preparation of the present invention is a step of preparing the pharmaceutical preparation and allowing it to be fermented. At this time, water can be added or an additional sterilization treatment can be performed.
Fermentation step of the pharmaceutical mixture
The step of fermenting the pharmaceutical preparation of the present invention is a step of inoculating a strain into the pharmaceutical preparation and then fermenting the same.
The method of the present invention for preventing hair loss and promoting hair growth may further comprise the step of extracting the fermented product after the fermentation step. The extraction step may be performed by heat extraction or hot water extraction of the fermented product.
Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. It should be understood, however, that the invention is not limited to the disclosed embodiments, but is capable of many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.
≪ Materials and methods >
material
The ginseng seeds, green leaves and licorice were purchased from Kyungdong Market (Seoul) and stored in refrigerator.
The combined strains were Lactobacillus adicophilus DDS-1, Lb. brevis KCTC 13094, Lactobacillus plantarum subsp. Plantarum (Lactobacillus plantarum subsp. Plantarum) KCTC3106 were each prepared in the same volume in a liquid medium and used in the experiments of the present invention.
Manufacture of complex extracts
(Weight basis) were mixed with each other in the same manner as in Example 1. The mixture was mixed with water at a weight ratio of 1:10 and then subjected to hot extraction at 95 to 100 ° C for 6 hours. The hot-water extract was filtered using a filter cloth, and the filtrate was concentrated twice using a vacuum rotary condenser. The concentrate was used as a complex extract in the following experiments.
complex Fermented Produce
(Weight basis) were mixed with each other in the same manner as in Example 1. The mixture was mixed with water at a weight ratio of 1:10 and sterilized by autoclave (1.5 atm, 121 ° C, 15 minutes). Then, the bacterial strain (Lactobacillus adicopilus DDS-1, Lactobacillus brevis KCTC 13094 and Lactobacillus plantarum subsp. Plantarum KCTC3106) was inoculated into the sterilized mixture and cultured at 30 ° C for 5 days to effect fermentation Water was obtained. The fermented product was extracted by heating at 95 to 100 DEG C for 6 hours. The extract was filtered using a filter cloth, and the filtrate was concentrated twice using a vacuum rotary condenser. The concentrate was used as a composite fermented product.
mouse
Six-week-old C57BL / 6 male mice were obtained from BioLink and grown under conditions of a temperature of 23 ± 3 ° C and a relative humidity of 50 ± 10% for 12 hours. At this time, dietary and drinking water were freely ingested and used for the experiment after 7 days adaptation period. Four groups of 5 mice were used for each sample. Twenty experimental animals were used.
All animal experiments were conducted according to the Ethics Guidelines of the Korea University Animal Experimental Ethics Committee (KUIACUC-2014015-2).
Hair growth experiment
The mice were anesthetized by intraperitoneal injection of pentobarbital (50 mg / kg, Entobar, Hanlim Pharm.) And then hair was removed from the back of the mouse using a hair clipper. In order to remove the hair follicles and fine hairs remaining in the skin, hair removal agents (NiClin, Ildong Pharma) were applied to the skin and left for 5 minutes. Then, a hair removal spatula was used. The epilated C57BL / 6 male mice were washed with lukewarm water to remove the hair removal agent, and after recovery for 24 hours, they were separated into normal (control), experimental (S-1, S-2) and minoxidil Each drug was applied 200 μl per day to the epidermis of the epidermis once daily. 5% minoxidil solution in which physiological saline was dissolved in normal group and minoxidil group in extraction solvent and compound extract (S-1) and complex fermented product (S-2) in test group were applied.
Analysis of hair growth effect by gross evaluation
On the 7th, 14th, and 21st days after the start of the experiment, the hair was lightly anesthetized to check for hair growth and then taken using a digital camera. The hairy condition was visually judged, scored and compared by group; 0% to 20% (0), 20% to 40% (1), 40% to 60% (2), 60% to 80% (3), 80% to 100% (4).
The mean value of each group was calculated. On the 21st day of the experiment, 10 hairs per head were extracted from the dorsal area and the average hair length and hair weight were measured.
Measurement of enzymatic activity of skin tissue
At the 3rd week of experiment, the skin tissue was cut and made into a micro slice under ice-cooling. A predetermined amount of the micro-slices was weighed, and a homogenate was prepared using a homogenizer by adding 4 times (by weight) phosphate buffer solution (PBS) to the skin tissue. The homogenate was centrifuged at 12,000 rpm for 20 minutes at 4 ° C using a centrifuge. After centrifugation, the supernatant was analyzed for alkaline phosphatase (ALP) and γ-Glutamyl transpeptidase (γ-GT) using an automatic biochemical analyzer (Konelab 20XT, Thermo, Finland).
Molecular Biological Analysis Using Real-Time Quantitative Polymerase Chain Reaction
To investigate the expression of IGF-1, VEGF, hair growth inhibitor and hair growth factor TGF-β1, known as hair growth factors, real-time polymerase chain reaction (PCR) (Ha, WH, Park, DH, Efficacy of seaweed extract on hair growth promotion in experimental study of C57BL / 6 mice. Archives of
Statistical processing
One-way ANOVA was performed using SPSS 18.0 for Windows (SPSS Inc., USA) statistical program. Duncan's multiple range test was used to test the differences between the groups. The statistical significance of this study was p <0.05.
<Experimental Example 1> Analysis of compositional change according to fermentation time
The compositional change of the complex fermented product prepared in the < preparation of composite fermented product > according to fermentation time was analyzed.
As a result, it was confirmed that the number of bacteria increased continuously after 5 days. The content of total sugar tended to decrease with fermentation and uronic acid, polyphenols and flavonoids were continuously increased until fermentation for 5 days. And thereafter increased slightly thereafter. The change of active substance showed similar tendency to the increase of bacterial count. As a result of measuring the changes in the components during the fermentation process, it was judged that the fermentation of the complex fermented product was most suitable on the 5th day of fermentation. Therefore, the following experiments were conducted using the complex fermented product fermented for 5 days (FIG.
<Experimental Example 2> Analysis of compositional change according to fermentation
The compositional difference between the complex extract and the complex fermented product was analyzed.
As a result, the total sugar content of the complex extract was 235.6 mg / ml, whereas the complex fermented product was 65.4 mg / ml, indicating that the content of total sugar decreased by fermentation. On the other hand, uronic acid, polyphenols, and flavonoids, which are the active ingredients of plant material, are 8.9 mg / ml, 648.7 μg / ml and 203.3 μg / ml, respectively, Respectively. It was presumed that the active substances contained in plant cells were liberated through the fermentation process (Table 1).
<Experimental Example 3> Visual evaluation of fermentation effect
The C57BL / 6 mouse is the most widely used model in hair research because it has the advantage of having human-like black hairs that can easily determine the growth cycle of hair growth by color. Since the genetic characteristics of the hair cycle entering the dormancy from 6 weeks of age are well known, there are many advantages to starting the experiment at this time.
After depilating the mouse, the composite extract and the composite fermented product of the present invention were applied daily, and the aspect of hair growth was visually observed and recorded using a digital camera.
As a result, on the 1st day after application of the sample, the skin color of the back of all the groups became bright red, and the hair growth in all groups could not be observed even on the 7th day after application of the sample. However, at the 14th day after application of the sample, hair growth began in the entire skin, which was hair removed from the minoxidil group and the complex fermentation group (S-2). In the S-1 group, But the remaining three had black skin. On the other hand, some skin was black in the control group. On the 14th day after application of the sample, differences between the groups were confirmed. On the 21st day, the minoxidil group and complex fermented product group (S-2) showed overall hair growth, while the complex extract group (S-1) showed hairiness in some rats, but the hair growth progression in the control group was minimal 2).
(S-1), combined fermented product group (S-2) and minoxidil group showed higher hair growth than the control group. Particularly, the S-2 and minoxidil groups with the composite fermented product showed the highest values (Table 2).
Each value represents the mean ± standard deviation (n = 5).
Hair regrowth scoring index: 0% -20% (0), 20% -40% (1), 40% -60% (2), 60% -80% 100% (4).
a) p < 0.05, significantly different from the control group
<Experimental Example 3> Evaluation of fur length and hair weight
The hairs were extracted from the hair of the hair of the mice of Experimental Example 2, and hair length and hair weight were measured.
As a result, hair length in the 4th week of experiment was long in the order of the minoxidil group, the positive control group, S-1 and S-2, and the control group, respectively, and there was a significant difference between the groups. Especially, there was significant difference between S-2 and minoxidil group which was not different by visual observation.
As for hair weights, S-1, which is a control group and an experimental group, was not detected in the whole area such as S-2 and minoxidil group but only hair of hairy area was measured. Therefore, S-2 and minoxidil group There is no meaning in comparison. Nevertheless, the control and experimental groups, S-1, measured only the hair of the hairy area, and these hair were also significantly different from the hair of the S-2 and minoxidil groups. Significant differences were also noted between the S-2 and minoxidil groups (p <0.05) (Table 3).
Each value represents the mean ± standard deviation (n = 5).
Control group: saline, S-1: complex extract, S-2: complex fermented product, minoxidil: 5% minoxidil
A and b in the same row are significantly different in ANOVA and Duncan's multiple range tests (p <0.05).
<Experimental Example 4> Variation of enzyme activity of skin tissue
For the mice of Experimental Example 2, the skin tissue was cut at the 3rd week of experiment and the enzyme activity fluctuation was evaluated using the cut.
The activity of ALP in the skin tissue was measured to be 498.70 IU / mg in the minoxidil group and 453.50 IU / mg in the test group S (FIG. 3A). The activity of minoxidil was 55.1% in the minoxidil group and 41.1% and 24.1% in the S-1 and S-2 groups, respectively (p <0.05).
As a result of measuring γ-GT activity in skin tissues, the minoxidil group (52.42 IU / mg) showed about 51.0% higher activity than the control group (34.72 IU / mg), while S-1 (39.15 IU / mg) ) Group. γ-GT activity was high in the order of minoxidil>S-2>S-1> control group (FIG. 3B).
ALP, which is mainly involved in angiogenesis, is an index that smoothes the hair growth by supplying nutrients to capillary vessels densely packed in the dermal papilla. It can indirectly estimate the hair growth by ALP measurement. (Iida, M .; Matsuzaki, T., Hair cycle-dependent changes in alkaline phosphatase activity in the mesenchyme and epithelium in mouse volvulus follicles. Development, growth & differentiation 2007, 49, (3), 185-195.). As a result of the measurement of the enzymatic activity of ALP in the skin of the present experiment, a dose - related increase was observed in the experimental group, and it was considered that the increase of ALP by the test substance affected the hair growth effect.
γ-GT is an enzyme that catalyzes the synthesis of keratin protein by separating cysteine from glutathione or tripeptide, and is expressed as one of the hair growth marker components when the growth period of hair growth cycle is induced (Hamada, K .; Suzuki, K., Evaluation of biochemical indices as a hair cycle marker in C3H mice. Experimental Animals 1996, 45, (3), 251-256.). In this experiment, γ-GT activity in the skin tissue was not significantly different in the experimental group S-1, but S-2 was significantly increased in comparison with the control group, indicating that the composite fermented product also influenced the hair growth effect.
<Experimental Example 5> Effect of complex extracts and complex fermented products on expression of hair growth factor-related genes
Hair growth promotes growth in the dermis papillae, the core part of growth, and the affected dermal papilla cells release growth factors or inhibitors into the hair follicle epithelial cells, which then circulate in a state of growth and degeneration. Known growth factors include insulin-like growth factor (IGF) -1 and vascular endothelial growth factor (VEGF). In this experiment, changes in IGF-1 and VEGF expression by compound extract (S-1) and complex fermented product (S-2) were observed on the 21st day after application of the sample.
<5-1> Evaluation of expression of IGF-1
Insulin-like growth factor (IGF) -1 is known to promote the growth of hair follicles and epithelial cells cultured in vitro, prevent cell death of hair cells, and promote hair growth. In addition, there is a report that hair growth is significantly increased in IGF transgenic animals (Tang, L .; Bernardo, O .; Bolduc, C .; Lui, H.; Madani, S.; Shapiro, J., The expression of insulin-
As a result, an increase in the expression of IGF-1 was apparent in the minoxidil group and the S-2 group. The expression of IGF-1 by minoxidil was 3.73-fold higher than that of the normal group and that of the group S-2 treated with complex fermentation was increased by 3.47-fold (FIG. 4).
≪ 5-2 > Evaluation of expression of VEGF
VEGF is known as one of the typical growth factors for vascular endothelial growth factor. (Li, LF; Guo, J .; Gao, ZF, Overexpression), which is known as a factor that enhances hair growth and differentiation of hair follicle cells by improving blood circulation by growing vascular endothelium Clinical and experimental dermatology 2003, 28, (4), 429-33). In order to confirm whether the hair growth promoting effect of the combined extracts and complex fermented products was related to blood circulation, the expression of VEGF was confirmed by using the tissue on the 21st day after the start of application of the test substance.
As a result, it was confirmed that VEGF expression was increased in S-2 group, and VEGF expression level in S-2 group was higher than minoxidil group, but there was no significant difference. The expression of VEGF by minoxidil was 2.3-fold higher than in the normal group and 3.3-fold higher in the group treated with S-2 (FIG. 5).
<5-3> Evaluation of Expression of TGF-β1
It is known that TGF-β1 induces hair loss through the mechanism of inhibiting growth by allowing growth hair to enter the regressor. TGF-β1 has been shown to interfere with the development and maturation of hair follicles (Tang, L .; Bernardo, O., Bolduc, C .; Lui, H.; Madani, S .; Shapiro, J., The expression of insulin-
In this experiment, the effect of the complex extract and complex fermented product of the present invention on the expression of TGF-β1 was measured.
As a result, the expression level of TGF-β1 was increased by 10% in the combined extract and complex fermented product groups, but there was no significant difference. In the minoxidil-treated group, the expression level of TGF-β1 was decreased by 13% as compared with the control group, but there was no significant difference. The expression level of TGF-β1 was not significantly different between the control and experimental groups and the minoxidil treatment group, which was a positive control (FIG. 6).
<5-4> Conclusion
The results of <5-1> to <5-3> are summarized as follows. In addition to the change of hair growth, hair density and thickness evaluated by naked eyes, as well as the expression of hair growth factors IGF-1 and VEGF The S-2 group, which is an experimental group, has an effect comparable to that of the positive control group, minoxidil. Based on the results of molecular biology, it is presumed that the mechanism of complex extracts and complex fermented products is promoted by regulating growth factors such as IGF and VEGF, thereby promoting growth of hair follicles and surrounding tissues and improving blood circulation.
Claims (10)
Wherein said medicinal herb contains 50 to 200 parts by weight of the top leaf, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the peppermint, 50 to 200 parts by weight of the plantain, 50 to 200 parts by weight of camellia seeds, 50 to 200 parts by weight of ginseng seeds, 50 to 200 parts by weight of green tea, and 50 to 200 parts by weight of licorice,
The fermentation product of the medicinal mixture is obtained by inoculating the pharmaceutical mixture with a strain selected from the group consisting of Lactobacillus adicopilus, Lactobacillus brevis and Lactobacillus flutarium, and fermenting at 25 to 35 DEG C for 2 to 10 days By weight of the composition.
Wherein the fermented product of the medicinal mixture is prepared by inoculating a strain into the medicinal mixture and fermenting the same to extract it.
Wherein said medicinal herb contains 50 to 200 parts by weight of the top leaf, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the peppermint, 50 to 200 parts by weight of the plantain, 50 to 200 parts by weight of camellia seeds, 50 to 200 parts by weight of ginseng seeds, 50 to 200 parts by weight of green tea, and 50 to 200 parts by weight of licorice,
The fermentation product of the medicinal mixture is obtained by inoculating the pharmaceutical mixture with a strain selected from the group consisting of Lactobacillus adicopilus, Lactobacillus brevis and Lactobacillus flutarium, and fermenting at 25 to 35 DEG C for 2 to 10 days ≪ / RTI >
A food composition for preventing hair loss and promoting hair growth.
Wherein the fermented product of the medicinal substance mixture is prepared by inoculating a strain into the medicinal substance mixture, fermenting the same, and extracting it.
Wherein said medicinal herb contains 50 to 200 parts by weight of the top leaf, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the perennial plant, 50 to 200 parts by weight of the peppermint, 50 to 200 parts by weight of the plantain, 50 to 200 parts by weight of camellia seeds, 50 to 200 parts by weight of ginseng seeds, 50 to 200 parts by weight of green tea, and 50 to 200 parts by weight of licorice,
The fermentation product of the medicinal mixture is obtained by inoculating the pharmaceutical mixture with a strain selected from the group consisting of Lactobacillus adicopilus, Lactobacillus brevis and Lactobacillus flutarium, and fermenting at 25 to 35 DEG C for 2 to 10 days ≪ / RTI >
A pharmaceutical composition for preventing hair loss and promoting hair growth.
Wherein the fermented product of the medicinal substance mixture is prepared by inoculating a strain into the medicinal substance mixture, fermenting the same, and extracting the fermented product to thereby prevent hair loss.
Wherein the fermentation product of the medicinal substance mixture is inoculated to the medicinal substance mixture at a temperature ranging from 25 to 35 DEG C for 2 days to 5 days at a temperature of < RTI ID = 0.0 > 25 C < / RTI >≪ RTI ID = 0.0 > 10 < / RTI >
A method for preparing a cosmetic composition for preventing hair loss and promoting hair growth.
Wherein the fermentation product of the medicinal substance mixture is inoculated to the medicinal substance mixture at a temperature ranging from 25 to 35 DEG C for 2 days to 5 days at a temperature of < RTI ID = 0.0 > 25 C < / RTI >≪ RTI ID = 0.0 > 10 < / RTI >
A method for producing a food composition for preventing hair loss and promoting hair growth.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106821907A (en) * | 2017-02-14 | 2017-06-13 | 玉林祺钟商贸有限公司 | A kind of efficient cosmetic hair care and preparation method |
| WO2018062751A1 (en) * | 2016-09-27 | 2018-04-05 | (주)아모레퍼시픽 | Composition containing lysate of lactobacillus plantarum for hair or scalp |
| KR102137252B1 (en) * | 2020-04-23 | 2020-07-24 | 주식회사 제이투케이바이오 | fermented extract for preventing stress alopecia using Lactobacillus plantarum J2K-27, and manufacturing method of it, and cosmetic composition containing fermented extract |
| KR20220107964A (en) | 2021-01-26 | 2022-08-02 | 주식회사 리스큐어바이오사이언시스 | A Composition for promoting hair growth comprising extracellular vesicles derived from Lactobacillus curvatus as active ingredients |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101267198B1 (en) | 2012-08-15 | 2013-05-27 | 김진옥 | A method which manufactures shampoo for preventing loss of hair and promoting growth of hair |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101267198B1 (en) | 2012-08-15 | 2013-05-27 | 김진옥 | A method which manufactures shampoo for preventing loss of hair and promoting growth of hair |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018062751A1 (en) * | 2016-09-27 | 2018-04-05 | (주)아모레퍼시픽 | Composition containing lysate of lactobacillus plantarum for hair or scalp |
| CN106821907A (en) * | 2017-02-14 | 2017-06-13 | 玉林祺钟商贸有限公司 | A kind of efficient cosmetic hair care and preparation method |
| KR102137252B1 (en) * | 2020-04-23 | 2020-07-24 | 주식회사 제이투케이바이오 | fermented extract for preventing stress alopecia using Lactobacillus plantarum J2K-27, and manufacturing method of it, and cosmetic composition containing fermented extract |
| KR20220107964A (en) | 2021-01-26 | 2022-08-02 | 주식회사 리스큐어바이오사이언시스 | A Composition for promoting hair growth comprising extracellular vesicles derived from Lactobacillus curvatus as active ingredients |
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