KR20230103028A - Composition for prevention of hair loss or improvement of hair growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or stimulating hair growth. It has an excellent effect of promoting the activity of the wind/beta-catenin signaling pathway, and thus can be usefully used as a composition for preventing hair loss or promoting hair growth.

Description

Composition for prevention of hair loss or improvement of hair growth}

The present invention relates to a composition for preventing hair loss or promoting hair growth, and specifically, by containing cacao extract as an active ingredient, inducing proliferation of dermal papilla cells to promote hair growth and inhibiting the activity of 5-alpha-reductase to dehydrogenate It relates to a composition for preventing hair loss or stimulating hair growth, which has an excellent effect of inhibiting testosterone production as well as promoting the activity of the wint/beta-catenin signaling pathway.

In addition to the simple function of biologically protecting the scalp, hair has been considered important as a means of expressing beauty and individuality in terms of beauty. In particular, in a modern society that values beauty, hair occupies the most important part in satisfying the desire for individual expression regardless of age or gender.

In recent years, not only male pattern baldness, but also a change in dietary habits and an increase in stress caused by social environments have led to an increase in the number of female hair loss populations, and their age is also decreasing regardless of age or gender. Moreover, in recent years, as the regulation or restriction of internal and external activities has been followed by the prolonged corona pandemic, the number of hair loss population due to increased stress, lack of exercise, irregular lifestyle, etc. is rapidly increasing.

There are about 100,000 to 150,000 hairs in the human body, and each hair has a different cycle and repeats growth and shedding through anagen, catagen, and telogen. This hair growth cycle is repeated over 3 to 6 years. As a result, it is a natural phenomenon that about 50 to 70 hairs fall out per day, but if the number of hairs that fall out exceeds 100, it is likely to be a symptom of hair loss.

In general, 'alopecia' refers to an increase in the number of abnormally falling hairs due to a decrease in the proportion of hair in the growth phase and an increase in hair in the catagen or telogen phase during this cycle. Alopecia, in which hair is lost from the scalp, is caused by various factors, and is usually divided into congenital genetic predisposition and acquired alopecia formed by receiving other environments. Currently, the causes of hair loss include internal factors such as the action of male hormones or external factors such as mental stress in daily life and accumulation of lipid peroxide in the scalp. there is.

Specifically, in terms of the hair growth cycle, alopecia refers to an increase in the number of hairs that fall out abnormally due to a shortened hair growth rate in the hair growth cycle and an increased number of hairs in the catagen or telogen phase. Hair loss is a normal phenomenon, but normal people have a lot of hair in the growth phase, whereas people with alopecia have a lot of hair in the resting phase. In other words, the characteristic of people exhibiting alopecia is miniaturization of the hair. As hair loss progresses, the period of the growth phase is shortened, the transition to the catagen phase and the telogen phase is promoted, and the volume of the subsequent hair dermal papilla is reduced, so the hair follicles are gradually miniaturized. Therefore, for the treatment of hair loss, it may be a more preferable method to allow hair follicles in the telogen phase to quickly go to the anagen phase and to increase the shortened anagen phase.

Hair loss prevention agents that can improve or treat alopecia that are currently widely used include minoxidil and oral finasteride, which are external skin preparations approved by the US Food and Drug Administration (FDA), and approved by the Ministry of Food and Drug Safety (MFDS) in Korea. There is an oral dutasteride that has been received. Minoxidil, which has been used as a treatment for hypertension, has been known to promote hair growth through a mechanism that expands blood vessels through hyperpolarization of cell membranes and supplies nutrients to hair follicles, but recent studies have shown that minoxidil promotes hair growth by acting directly on hair follicle cells. It has been reported to activate the signaling pathway of Wnt/β-catenin, which is a mechanism. Finasteride is the ingredient name of Propecia, which was developed as a treatment for benign prostatic hyperplasia and then introduced to the treatment of alopecia. ) is known to inhibit the production of DHT, which causes hair loss. However, it is true that anti-hair loss drugs such as minoxidil, finasteride, dutasteride, etc. show a large effect temporarily in terms of efficacy, but the effect varies greatly between individuals, and the effect disappears immediately when discontinued, and various dermatitis when used for a long time However, as many side effects such as loss of libido and induction of gynecomastia are concerned, it is difficult to maintain these effects for a long period of time.

Cacao (scientific name: Theobroma cacao L.) is the fruit of the cacao tree, a perennial plant belonging to the family Sterculiaceae. It is also widely cultivated in tropical regions such as West Africa, Nigeria, Ghana, Sri Lanka, and Indonesia. The cacao tree is a tall tree reaching 10 to 15 m in height, and the fruit is spindle-shaped with a length of 15 to 25 cm and contains 40 to 60 seeds (or seeds) in the fruit.

The name cacao was originally the name of a cacao tree, which was initially used to refer to unfermented pods, but recently matured seeds made by picking only seeds from cacao trees and fermenting them moderately at 45°C or less for 3 to 9 days. , In other words, it refers to the seeds in the fruit that are fermented, washed and then dried. Today, cacao generally refers to cacao beans.

Cacao beans contain fat (35-50%), dietary fiber (about 16%), protein (about 11%), as well as alkaloids (1.3-2%), including theobromine (2%) and a small amount of caffeine. %) and other phenolic compounds (10%), flavanol-3-ol monomers such as (+)-catechin and (-)-epicatechin), which are phenolic compounds, and Dimers such as procyanidin B1 and procyanidin B2 and various oligomers such as anthocyanins and proanthocyanidins are contained. In particular, it contains about 3.6 times more antioxidant polyphenols than green tea and about 60 times more catechins than green tea, and the content of flavonoids is much higher than that of green tea, almonds, and hazelnuts.

As such, antioxidant components such as flavonoids and polyphenols contained in cacao beans promote blood circulation and strengthen blood vessel walls, which has a great preventive effect on various heart diseases. It is known to be effective in dieting by promoting the decomposition of body fat. In addition, recently, the effect of reducing the LDL (low density lipid) oxidation and lipoxydase activity of procyanidin based on cacao-derived polyphenols, the dose-dependent effect of chocolate consumption on plasma oxidative damage, and free radical-induced red blood cells Many studies on antioxidant efficacy, such as the inhibitory effects of flavanols and procyanidins on hemolysis, are being actively conducted.

Regarding the efficacy of the cacao extract, Korean Registered Patent Nos. 10-1902988 and 10-0973782 disclose an effect of improving skin conditions and promoting skin cell proliferation, but examples of use for the efficacy of preventing hair loss or promoting hair growth It was not known.

The inventors of the present invention have made diligent efforts to develop a composition that can prevent hair loss, induce hair growth, and effectively act on safe hair loss prevention or hair growth promotion without side effects even when used on the human body for a long time. It was confirmed that there was, and came to complete the present invention.

Accordingly, the technical problem to be achieved by the present invention relates to a composition for preventing hair loss or stimulating hair growth, by containing cacao extract as an active ingredient, promoting hair growth by inducing proliferation of dermal papilla cells and inhibiting the activity of 5-alpha-reductase. It is an object of the present invention to provide a composition for preventing hair loss or stimulating hair growth, which has an excellent effect of suppressing the production of dihydrotestosterone by inhibiting and promoting the activity of the wint/beta-catenin signaling pathway.

In order to achieve the above object, the present invention provides a composition for preventing hair loss or promoting hair growth containing cacao extract as an active ingredient,

In order to achieve the above object, the present invention contains cacao extract as an active ingredient to promote hair growth by inducing proliferation of dermal papilla cells and inhibiting the production of dihydrotestosterone by inhibiting the activity of 5-alpha-reductase. Of course, it provides a composition for preventing hair loss or promoting hair growth that promotes the activity of the wint / beta-catenin signaling pathway.

By containing the cacao extract of the present invention as an active ingredient, there is no skin irritation or cytotoxicity, so it has excellent human safety, induces the proliferation of dermal papilla cells to promote hair growth, and inhibits the activity of 5-alpha-reductase to produce dihydrotestosterone. Since it is very effective in promoting the activity of the wint/beta-catenin signaling pathway as well as inhibiting, it can be usefully used as an active ingredient in cosmetic, pharmaceutical and health functional food compositions for preventing hair loss or promoting hair growth.

1 is a graph showing the analysis results for the activity promoting effect of the wint/beta-catenin signaling pathway by the cacao extract of the present invention.

The present invention relates to a composition for preventing hair loss or promoting hair growth containing cacao extract as an active ingredient.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is one well known and commonly used in the art.

Hereinafter, the present invention will be described in more detail.

In the present invention, cacao refers to cacao beans obtained by taking out only seeds from cacao trees, fermenting, washing with water, and drying.

In the present invention, the cacao bean is a cacao tree ( Theobroma cocoa L.) species, and currently, there are about 20 cacao varieties and hundreds of hybrids in the genus Theobroma, among which Forastero cacao, Creole, which is commercially distributed in the market Criollo cacao, Trinitario cacao, and Nacional cacao, as well as four hybrid fruit seeds, are included.

In the present invention, cacao beans are morphologically composed of outer skin, inner skin, endosperm, embryo, etc., and cacao nibs are fermented, dried, roasted (roasted) cacao beans, and then separated from the shell (outer skin) It refers to the finely crushed kernels (endodermis). At this time, the content of cacao nibs is considered to be about 85% of the total content of cacao beans. Afterwards, the paste phase made into a liquid form by crushing the cacao nibs is called cocoa mass (cocoa liquor or cocoa bitter), and the cocoa mass is again made of cacao butter, which is fat, and the remaining fat-free part. It is separated into cocoa and used for product utilization.

In the present invention, cacao beans include cacao nibs, which are kernels (endodermis), in which the fermented seeds are dried and roasted, and then the skin (outer skin) is peeled off, and then crushed into an appropriate size through a re-roasting process. , It is not limited to the type of cacao beans.

In the present invention, the cacao extract refers to an extract obtained by a specific extraction method from both cacao bean skins and cacao nibs exfoliated with the cocoa beans, a diluted or concentrated solution of the extracts, a dried product obtained by drying the extracts, It refers to extracts of all formulations that can be formed using the extract itself and the extract, such as a preparation or a purified product of the extract, or a mixture thereof.

In the present invention, the cacao extract can be extracted from natural, hybrid or mutant plants of the cacao bean, and can also be extracted from plant tissue culture.

In the present invention, a method for extracting cacao extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods. In addition, as long as the effective activity of the useful components of the extract is not removed, the temperature is not particularly limited.

In addition, the cacao extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract subjected to conventional purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using ultrafiltration membranes with a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity) Active fractions obtained through various additional purification and extraction methods, such as separation by a method for separation according to sex) or extracts obtained by natural conditions or fermentation products using various microorganisms, are also included in the extract of the present invention.

The type of extraction solvent used in the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, anhydrous or hydrous lower alcohols having C1 to C4 carbon atoms (eg methanol, ethanol, propanol and butanol), a mixed solvent of the water and lower alcohols, propylene glycol, butylene glycol, At least one selected from the group consisting of glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, and hexane, and may be used alone or in combination of two or more.

According to one embodiment of the present invention, the cacao extract may be prepared according to a conventional method known in the art, that is, under conventional temperature and pressure conditions, using a conventional solvent, but preferably the solvent An extract can be prepared by hot water extraction of cacao bean hulls and cacao nibs one or more times using, and a dried extract obtained by freeze-drying or spray-drying after distillation of the solvent extract under reduced pressure can be prepared. In a specific embodiment of the present invention, reflux extraction was performed to obtain cacao bean husk and cacao nibs extract.

In the present invention, the extract obtained by heat extraction or cold extraction is filtered to remove floating solid particles by filtering out particles using, for example, nylon, or by using cryofiltration, and then used as is or freeze-dried, hot-air-dried, It can be dried using spray drying or the like.

Therefore, the extraction method of cacao extract promotes hair growth by inducing the proliferation of dermal papilla cells, which is the object of the present invention, inhibits the activity of 5 alpha-reductase to inhibit the production of dihydrotestosterone, as well as wint/beta - It is not particularly limited as long as it is sufficient to exhibit the characteristics of the effect of promoting the activity of the catenin signaling pathway.

Although many studies on the cause of hair loss or promoting hair growth are continuously and actively conducted, the exact biological mechanism related to hair loss is still very complicated.

Hair repeats the hair cycle of growth, catagen, rest, and growth, and alopecia refers to a state in which the resting period is long and the growth period is short. It has been believed that hair regeneration is almost impossible until now because hair follicles atrophy when the resting period is long, and most of the hair loss prevention agents currently on the market expect only the hair loss prevention effect of growing hair.

Hereinafter, the invention for preventing hair loss or promoting hair growth of the present invention will be described in detail.

In the present invention, "hair loss" refers to a state in which there is no hair in an area where hair should normally exist, and specifically means that the terminal hair (thick and black hair) of the scalp is lost, and various forms from slight thinning of hair to complete baldness appears as The cause of hair loss is not limited, but examples include hereditary androgenetic alopecia (baldness), alopecia areata, ringworm of the head (tinea capitis) caused by fungal infection, telogen effluvium, hair growth, hair production disorders, etc. Scarring alopecia may mean that it appears in various forms due to various causes, such as hair loss caused by lupus, idiopathic folliculitis, lichen planus pilaris, hair loss caused by burns and trauma.

In the present invention, the types of hair loss include scarring alopecia in which hair is not regenerated due to the destruction of hair follicles due to scarring, and non-scarring alopecia in which hair is regenerated after the symptomatic site is razed by maintaining hair follicles without forming scars. Scarring alopecia is caused by trauma, burns, leprosy or bacterial infection, and non-scarring hair loss includes alopecia areata, hair growth, telogen effluvium, drug-induced alopecia, secondary syphilis, dermatophytosis, endocrine disorders, and dermatomyositis. which is caused by, etc.

The term "prevention" of the present invention refers to preventing something or a phenomenon from occurring, that is, preventing hair loss from occurring, and a product that prevents hair loss along with hair growth is called an anti-hair loss agent. In addition, it is divided into a hair growth agent that nourishes the hair roots to grow hair, a hair growth agent that promotes blood circulation in the scalp to help prevent hair loss, and a hair growth agent that turns thinned vellus hair into healthy terminal hair.

In the present invention, the target of application of the anti-hair loss agent means that the focus is on scarring hair loss. Among scarring alopecia, androgenetic alopecia, in which male hormones act as an important etiology, accounts for about 80% of all hair loss symptoms. Genetic factors, the concentration of androgen, a male hormone in the blood, and a high concentration of dihydrotestosterone (DHT: dihydrotestosterone), which binds to androgen receptors present in scalp hair follicles, are known to be the main causes. DHT regulates hair growth by binding to the androgen receptor in the scalp dermal papilla. At this time, the binding of DHT transmits a cell destruction signal to the genes of keratinocyte cells to produce apoptosis factors, DKK-1 and TGF-β. This means that hair loss occurs due to the destruction of hair cells.

In addition, in the present invention, 5 alpha-reductase (5α-reductase), an enzyme that catalyzes the irreversible reduction of testosterone to DHT, together with nicotinamide adenine dinucleotide phosphate (NADPH) as a hydrogen donor. reductase) is associated with hair loss. That is, DHT produced in this way inhibits protein synthesis around the hair follicle, thereby reducing cell proliferation of hair cells, shrinking scalp hair follicles, and converting thick and long healthy hair into thin and short hair to progress hair loss.

In the present invention, "hair growth" means that hair grows in a hair follicle, and the hair includes hair roots and hair follicles of the head, hair, eyelashes, eyebrows, beard, armpits, pubic hair, and all parts of the body with hair roots and hair follicles do.

In the present invention, "acceleration" means supplying nutrients to the root of the hair so that the hair grows quickly, thereby controlling the growth cycle of the hair. At this time, the activity of outer root sheath cells, including dermal papilla cells and hair follicle stem cells constituting hair follicles, and various cytokines and growth factors produced by these cells play an important role. In particular, regeneration of hair follicles and activation of stem cells are followed for periodic hair growth, which is achieved through activation of the Wnt/β-catenin signaling pathway. In addition, growth factors such as vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and fibroblast growth factor (FGF) promote the growth phase of hair, and transforming growth factor (TGFβ-1) , Epidermal growth factor (EGF), etc. means to regulate the hair growth cycle by inducing the catagen phase of the hair follicle.

Therefore, in the present invention, the hair loss prevention or hair growth promoting effect promotes dermal papilla cell proliferation by the cacao extract, inhibits the activity of 5 alpha-reductase, and promotes the activity of the wint/beta-catenin signaling pathway. may be achieved by

In an embodiment, the composition for preventing hair loss or promoting hair growth (or for promoting hair growth) of the present invention may be used as a cosmetic composition, a pharmaceutical composition (pharmaceutical composition, for quasi-drugs), or a health functional food composition. The composition of the present invention may be formulated in any form that can be applied to the skin in general, but may be formulated in the form of an external preparation for skin. The composition of the present invention may be formulated into a formulation that can be applied to the skin, such as, for example, liquid, cream, paste, or solid.

The cacao extract of the present invention may be included in the composition or formulation as an agent for preventing hair loss or a hair growth promoter.

According to one embodiment of the present invention, the composition containing the cacao extract of the present invention is formulated into a cosmetic formulation and can be used in various ways such as hair cosmetics having an effect of preventing hair loss or promoting hair growth.

In one embodiment of the present invention, in the cosmetic composition for preventing hair loss or stimulating hair growth, the content of the cacao extract is not particularly limited, but is preferably contained in an amount of 0.001 to 30.0% by weight based on the total weight of the composition. More preferably, it is contained in an amount of 0.1 to 10% by weight based on the total weight of the composition, and may be more preferably 1.0 to 5% by weight. However, when the cacao extract is less than 0.001% by weight, the hair growth promoting effect is insignificant, and when it exceeds 30% by weight, the stability of the formulation is impaired and skin irritation may occur.

In the cosmetic composition of the present invention, when the cacao extract is 1 to 5% by weight based on the total weight of the composition, it has been shown to have an effect of preventing hair loss or promoting hair growth in vitro or clinically. More preferably, the content of the cacao extract may be 1 to 10.0% by weight, most preferably 2.0 to 5% by weight, based on the total weight of the composition. In the present invention, it was confirmed through test examples that the effect of preventing hair loss or promoting hair growth was remarkably excellent when the cacao extract was within the above content range.

The cosmetic composition containing the cacao extract of the present invention is not particularly limited in formulation, and may be appropriately selected according to the purpose. For example, softening lotion (skin lotion and milk lotion), nourishing lotion, essence, nourishing cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, Body lotion, body cream, body oil, body essence, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair Massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dressing , hair lacquer, hair moisturizer, hair mousse and hair spray, but may be prepared in any one or more formulations selected from the group consisting of, but not limited thereto, preferably hair tonic, hair conditioner, hair essence, hair lotion, hair Nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair It can be formulated as a drying agent, hair preservative, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair mousse or hair spray.

When the composition containing the cacao extract of the present invention is formulated into a cosmetic formulation, the composition of the present invention may include all components commonly used in cosmetics in addition to the extract, such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, It may contain a component selected from the group consisting of polymeric polysaccharides, sphingo lipids, and seaweed extracts.

In the present invention, any polymeric peptide can be selected as long as it can be incorporated into cosmetics, but preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be selected. Polymer peptides can be purified and obtained by conventional methods such as a purification method from a culture medium of microorganisms, an enzyme method, or a chemical synthesis method, or can be used after being purified from natural products such as pig or cow dermis or silkworm silk fibers.

In the present invention, as the polymeric polysaccharide, any polysaccharide that can be blended with cosmetics can be selected, but hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) is preferably selected. can For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

In the present invention, any sphingolipid can be selected as long as it can be blended with cosmetics, but ceramide, phytosphingosine, sphingoglycolipid and the like can be preferably selected. Sphingolipids can be purified by conventional methods or obtained by chemical synthesis from mammals, fish, shellfish, yeast, or plants.

In the cosmetic composition of the present invention, in addition to the above essential ingredients, other ingredients commonly used in cosmetic preparations may be incorporated as needed. In this case, the ingredients include oil and fat components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, and blood circulation promoters. , cooling agents, antiperspirants, purified water and the like can be used.

Ingredients included in the composition formulated as a cosmetic formulation of the present invention may include ingredients commonly used in cosmetics in addition to the extract as an active ingredient, for example, stabilizers, solubilizers, vitamins, pigments, and fragrances Such conventional adjuvants and carriers are included.

The pharmaceutical composition in the present invention means prepared for the purpose of preventing or treating diseases, and the pharmaceutical composition containing the cacao extract of the present invention further includes appropriate carriers, excipients and diluents commonly used in the preparation of pharmaceutical compositions. can include

The pharmaceutical composition containing the cacao extract according to the present invention is formulated according to conventional methods, such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, sprays, external preparations, suppositories and sterile injection solutions, It may be formulated and used preferably in the form of a powder, granule, tablet or spray, more preferably in the form of a nasal spray, and diluents such as lubricants, wetting agents, flavoring agents, emulsifying agents, suspending agents, preservatives, surfactants, etc. It may be formulated into a parenteral formulation using an excipient and used.

In addition, it may be formulated and used in the form of external preparations, suppositories, external preparations for the skin, and sterile injection solutions. Specifically, the composition of the present invention can be used by a method such as directly applying or spreading to the hair or scalp. The hair to which the composition of the present invention is applied includes hair roots and hair follicles of the head, hair, eyelashes and eyelashes, beard, armpits, pubic hair, hair roots throughout the body, and all parts with hair follicles.

The pharmaceutical composition containing the cacao extract of the present invention can be administered in a pharmaceutically effective amount. The pharmacologically effective amount means an amount that is sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and does not cause side effects. It may be determined according to the activity of the drug, sensitivity to the drug, method of administration, time of administration, route of administration and excretion rate, duration of treatment, factors including drugs used in combination or concurrently, and other factors well known in the medical field.

Specifically, it is generally 0.01 mg to 5000 mg per day per 1 kg of body weight of the subject to be administered, and may be administered once or several times a day at regular time intervals according to the judgment of a doctor or pharmacist, but is not limited thereto. That is, the preferred dosage of the pharmaceutical composition containing the sialic acid-containing whey protein of the present invention varies depending on the condition and body weight of the patient, the severity of the disease, the type of drug, the route and duration of administration, but can be appropriately selected by those skilled in the art. . However, for desirable effects, the complex of the present invention is preferably administered at 0.2 to 200 mg per day, preferably 2 to 100 mg.

Administration may be administered once a day, or may be administered in several divided doses. The dosage is not intended to limit the scope of the present invention in any way.

The cacao extract of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes. All modes of administration can be envisaged, for example by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

In addition, the pharmaceutical composition containing the cacao extract of the present invention has little toxicity and side effects, so it can be safely used even when taken for a long period of time for the purpose of prevention or treatment.

In the present invention, the pharmaceutical composition may be used alone or in combination with other pharmaceutically active compounds exhibiting an effect of preventing or improving hair loss-related diseases, or in a suitable set.

The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And it can be single or multiple administrations. It is important to administer the amount that can obtain the maximum effect with the minimum amount without causing side effects in consideration of all of the above factors, and can be easily determined by those skilled in the art.

For example, but not limited thereto, in addition to the pharmaceutical composition containing the cacao extract of the present invention as an active ingredient, conventional anti-hair loss agents such as minoxidil, finasteride, spironolactone, or dutasteride can be selected for additional administration.

As used herein, the term "administration" means introducing the pharmaceutical composition of the present invention to a subject by any suitable method, and the route of administration of the composition of the present invention can be various oral or parenteral routes as long as it can reach the target tissue. can be administered through

The administration method of the pharmaceutical composition according to the present invention is not particularly limited, and may follow a method commonly used in the art. As a non-limiting example of the administration method, the composition may be administered by oral administration or parenteral administration. Specifically, the composition of the present invention can be used by a method such as directly applying or spreading to the hair or scalp.

The pharmaceutical composition according to the present invention may be prepared in various formulations depending on the desired administration method.

The frequency of administration of the composition of the present invention is not particularly limited thereto, but may be administered once a day or administered several times by dividing the dose.

As another aspect, the present invention provides a method for preventing or treating hair loss-related diseases comprising administering a pharmaceutical composition containing cacao extract.

In the present invention, diseases related to causes of hair loss include Alopecia Areata, Androgenetic Alopecia, Telogen Efffluvium, Traumatic, Trichoti lomania, Pressure Alopecia, etc. It may include, but is not limited to, nasal congestion, syphilitic (Alopecia syphlltiac), seborrheic alopecia (Alopecia seborrhecia), symptomatic, scarring, congenital alopecia, etc., and may refer to all diseases caused by hair loss from the hair follicle. there is

In the present invention, the subject of hair loss may refer to all animals, including humans, having or are likely to have a hair loss-related disease. The animal may be not only humans but also mammals such as cattle, horses, sheep, pigs, goats, camels, antelopes, dogs, and cats that require treatment for similar symptoms, but are not limited thereto.

Specifically, the preventive or therapeutic method of the present invention may include administering the composition in a pharmaceutically effective amount to a subject having or at risk of developing a hair loss-related disease. The administration method is as described above.

The prevention may mean any act of suppressing or delaying the onset of hair loss-related diseases by administering the composition for preventing hair loss or stimulating hair growth according to the present invention to a subject.

In addition, the treatment may refer to any action that improves or benefits the symptoms of a hair loss-related disease by administering the composition of the present invention to a subject suspected of having a hair loss-related disease.

For administration, the composition of the present invention may contain pharmaceutically acceptable carriers, excipients or diluents in addition to the above-described active ingredients. The carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

An appropriate dosage of the composition of the present invention varies depending on the condition and weight of the patient, the severity of the disease, the type of drug, and the time, but can be appropriately selected by those skilled in the art. Specifically, the dosage of the sialic acid-containing whey protein may be 50 to 500 mg.

The amount of the composition of the present invention can be appropriately adjusted according to individual differences such as age, severity of lesions, etc., or formulation, and it is preferable to apply an appropriate amount to the scalp once or several times a day and use it for one week to several months. In an embodiment of the present invention, the composition for preventing hair loss or stimulating hair growth was used 7 times a week for 3 months, and as a result, it was confirmed that an excellent effect of preventing hair loss or stimulating hair growth appeared (Formulation Example 2 and Test Example 5).

In the present invention, the food may be a 'functional food' or 'functional food', and may mean a food in which the functionality of a general food is improved by adding the cacao extract of the present invention, preferably the cacao nibs extract, to the general food.

In the present invention, functional effects can be roughly divided into physical properties and physiological functions. When the cacao extract of the present invention is added to general foods, the physical properties and physiological functions of the general foods will be improved, and the present invention provides Food can be comprehensively interpreted as 'functional food (health functional food)' or 'functional food (health functional food)'.

In the present invention, food refers to food manufactured and processed using raw materials or ingredients having useful functionalities for the human body according to Act No. 10219 on Health Functional Foods, which regulate nutrients for the structure and function of the human body or physiological It means to take it for the purpose of obtaining useful effects for health purposes such as medical action.

In the present invention, the type of food may be specifically a health functional food. The health functional food includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, organic acids, protective colloidal thickeners agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. These components may be used alone or in combination, and the proportion of these additives is generally selected from the range of 0.001 to 50 parts by weight per total weight of the composition.

In the present invention, the health functional food is a food that emphasizes the bioregulatory function of food, and is a food that has added value to act and express for a specific purpose using physical, biochemical, and bioengineering methods. The ingredients of these health functional foods are designed and processed to sufficiently exert the body's control functions related to body defense and regulation of body rhythm, prevention and recovery of diseases, and are food additives, sweeteners or functional foods acceptable as food. may contain raw materials.

In the present invention, the health functional food may be any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and pills, but is not limited thereto. Specifically, the health functional food in the form of a tablet is obtained by granulating a mixture of a composition containing cacao extract, excipients, binders, disintegrants, and other additives in a conventional manner, and then compression molding by adding a lubricant or the like, or directly It can be produced by compression molding. In addition, the health functional food in the form of a tablet may contain a bitter agent, etc., if necessary, and may be coated with an appropriate coating agent, if necessary.

In the present invention, hard capsules among capsule-type health functional foods can be prepared by filling a mixture of a composition containing cacao extract and additives such as excipients, or a granular material or coated granular material thereof in a conventional hard capsule. Soft capsules can be prepared by filling a mixture of a composition containing cacao extract and additives such as excipients into a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a colorant, and a preservative, if necessary.

In the present invention, the health functional food in the form of a ring can be prepared by molding a mixture of a composition containing cacao extract, an excipient, a binder, a disintegrant, etc. in an appropriate method, and, if necessary, a coating with white sugar or other suitable coating agent, or It can also be clothed with starch, talc or any other suitable material.

In addition, the health functional food in the form of granules can be granularly prepared by a mixture of a composition containing cacao extract, an excipient, a binder, a disintegrant, etc. by an appropriate method, and may contain a flavoring agent, a bitter agent, etc., if necessary. .

In the present invention, term definitions for excipients, binders, disintegrants, lubricants, bitter agents, flavoring agents, etc. are known in the art and may include the same or similar functions.

In addition, the type of food may be a food additive, and the food additive refers to a material used by adding, mixing, infiltrating, or other methods to food for manufacturing, processing, or preservation of food. The food additives include natural products and synthetic products, and can be classified according to functions and uses. Currently, 370 kinds of chemical synthetic products and 50 kinds of natural additives are permitted as food additives in Korea. They are mainly preservatives, fungicides, antioxidants, coloring agents, coloring agents, bleaching agents, seasonings, sweeteners, flavoring agents, expanding agents, reinforcing agents, and improvers depending on the use. , emulsifier, thickener (thickener) and stabilizer, coating agent, gum base agent, antifoaming agent, solvent, release agent, insect repellent, quality improver and other additives for food manufacturing.

In the present invention, the form of the food additive may include powder, granule, tablet, capsule or liquid form, and may specifically be in the form of a capsule, but is not limited to the above form.

In the present invention, when used as a food composition containing cacao extract, the cacao extract may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the cacao extract may be suitably determined according to its purpose of use (prevention, health or improvement, therapeutic treatment).

Hereinafter, preferred preparation examples, formulation examples, examples and test examples will be described in detail to aid understanding of the present invention. However, the following preparation examples, formulation examples, examples, and test examples are only for exemplifying the present invention and may be modified in various other forms, and the scope of the present invention is not limited only to the following examples, and equivalent It includes all variations of technological ideas. Preparation examples, formulation examples, examples and test examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

[Preparation Example 1] Preparation of cacao extract

In general, in the cacao extract manufacturing method, the cacao extract is the content of the cacao bean or the content of the cacao bean depending on the type of raw material for the origin of the cacao bean according to the type of cacao tree, the harvest time, fermentation conditions and methods, and the primary pretreatment processing method such as drying. Since the difference in components is large and its functional, nutritional and physiologically active characteristics can be different accordingly, in the present invention, more qualitatively commercialized cacao beans (harvested, fermented and dried in Ecuador in 2020) are purchased and used

The purchased cacao beans were roasted at 170 ° C. for 15 minutes using an oven, and then the peeled outer skin (hereinafter referred to as skin) and the inner skin (hereinafter referred to as cacao nibs) were separated and ground to a particle size of 1 mm or less with a miller to powder Powdered cacao bean shells and cacao nibs were frozen at -20 ° C and used as samples for this test.

4 to 10 parts by weight of 50% acetone and 50% ethanol were added to 1 part by weight of each of the powdered cacao bean hulls and cacao nibs, which were the samples, and extracted while refluxing at a temperature of 40 to 70 ° C. for 4 to 6 hours. Then, each extract was centrifuged to collect the obtained supernatant, and the remaining residue was repeatedly extracted 3 times and filtered using a 0.2 μm filter (Whatman No. 2). Each of the obtained filtrate was adsorbed on a styrene-based porous resin, and then fractionated with 20% ethanol and then sequentially added with 60% ethanol to obtain a solvent fraction. Each of these fractions was freeze-dried under reduced pressure and concentration to remove the solvent, and then cacao bean skin extract (Example 1) and cacao nibs extract (Example 2) were prepared, respectively. : 0.85 ratio mixed cacao extract prepared by mixing (Example 3) was also used in this test.

[Test Example 1] Proliferative effect of dermal papilla cells

In order to determine the effect of cacao extract on the proliferation of human hair follicle dermal papilla cells (HHFDPC), which is the main cell of hair growth, the proliferation capacity of dermal papilla cells was measured. Human dermal papilla cells were purchased from PromoCell, and the HHFDPC was supplemented with a culture medium additive (Supplement Mix; PromoCell, Heidelberg, Germany), 100 units/mL of penicillin, and 100 μg of streptomycin. Cell Growth Medium; PromoCell, Heidelberg, Germany) was cultured at 37°C and 5% CO ₂ conditions. Specifically, after culturing HFDPC, it was inoculated into a 96-well plate (1.5 x 10 ⁴ cells/well), and incubated in a 5% CO ₂ incubator at 37° C. for 24 hours. HFDPC cells were used in this test.

DMSO (Dimethylsulfoxide) diluted 1:1000 in DMEM (Dulbecco's modified Eagle's medium) containing 1% fetal bovine serum (FBS), penicillin 100 units/mL and streptomycin 100 μg 1 ng/mL βFGF to the cultured cells As a control, 0.1 μg of minoxidil was used as a positive control, and each concentration of 0.1, 0.5, and 1 μg was treated for Examples to Example 3, respectively, and then cultured again for 48 hours under 37 ° C. and 5% CO ₂ conditions. did After 72 hours, the cell culture supernatant was removed by centrifugation, washed once with PBS (Phosphate Buffer Saline) solution, and MTT (3-[4,5-dimethylthiazol-2-yl]-2 dissolved in 1 mg/mL ,5-diphenyltetrazolium bromide) reagent (Sigma, USA) was diluted 10 times, mixed with the medium, treated, and incubated for 1-2 hours, after which the MTT reagent was removed. When formazan crystals are observed in each well, 200 μl of DMSO per well is added and gently shaken for 30 minutes in a light-shielded state to dissolve the formazan crystals. The degree of cell proliferation was evaluated. CCK-8 was treated in a culture solution at a ratio of 1:10 and incubated for 1 hour, and then the absorbance at 450 nm was repeatedly measured three times using a spectrophotometer for each well, and the average of the absorbance values was calculated.

Table 1

The measurement results of the dermal papilla cell proliferation rate are shown in Table 1, and the results confirmed that the dermal papilla cell proliferation effect is shown in all treatments at concentrations of 0.1, 0.5, and 1 μg, respectively, for Examples 1 to 3. . Example 1 showed a relatively low dermal papilla cell proliferation rate compared to minoxidil at the same 0.1 μg treatment concentration as the positive control minoxidil, whereas Examples 2 and 3 showed a relatively higher dermal papilla cell proliferation rate than minoxidil. Significance between Examples 2 and 3 No difference was seen. In addition, it was shown that the pattern of dermal papilla cell proliferation rate at each of 0.5 and 1 μg treatment concentrations for Examples 1 to 3 was not significantly different from the result of 0.1 μg treatment concentration.

Therefore, it can be confirmed that the effect of promoting dermal papilla cell proliferation is not only much greater in cacao nibs extract than in cacao bean hull extract, but also superior to minoxidil.

[Test Example 2] Inhibitory effect of 5-alpha-reductase activity

In order to evaluate the activity of 5 alpha-reductase in dermal papilla cells, dermal papilla cells were dispensed at 0.8 x 10 ⁶ cells/dish in a 60 mm cell culture dish. After 24 hours of culture, the existing culture medium was removed, and no additives were added (0 mg/mL) as a control for each dish, finasteride (1 mg/mL) as a positive control, Examples 1 to 3 (1 mg/mL) were treated, respectively, and exchanged again with the culture medium containing each of them, and cultured for 24 hours. After culturing, the existing culture medium was removed, washed with 1 x PBS, and treated with Trypsin/EDTA to make each single cell. After centrifugation, the cells were lysed using an extraction buffer (880 mM Sucrose, 1.5M CaCl ₂ ), and then the supernatant obtained by centrifugation was taken and used as a crude enzyme solution for the measurement test of 5-alpha-reductase activity inhibition ability. used The measurement and analysis method calculated the activity of 5-alpha-reductase by measuring the change in the amount of NADPH, a substrate used when testosterone is converted to dihydrotestosterone, by absorbance, and the crude enzyme solution (protein 35 μg), 20 μM testosterone (Sigma, USA) and 320 μM NADPH (Sigma, USA) were mixed and incubated for 30 minutes, and then the absorbance of the mixture was measured at 340 nm using a spectrophotometer to obtain 5 alpha - The activity of the reductase was analyzed.

Table 2

As shown in Table 2 above, when finasteride, known to inhibit the activity of 5-alpha-reductase, was treated at a concentration of 1 mg/mL, a relative activity rate of 74.26±1.46% was shown, whereas in Example 1, finasteride While the activity rate of 5 alpha-reductase was relatively low compared to lead, Example 2 was calculated as 51.11 ± 1.98 and Example 3 was 50.73 ± 1.56, respectively, and no significant difference between Examples 2 and 3 was seen. These results indicate that both Examples 2 and 3 inhibit the activity of 5-alpha-reductase more effectively than finasteride.

Therefore, it can be confirmed that the effect of inhibiting the activity of 5-alpha-reductase is much greater in the cacao nibs extract than in the cacao bean hull extract, and significantly superior to finasteride.

[Test Example 3] Wint/beta-catenin signaling pathway activation effect

The method for measuring the activation level of dermal papilla cells, which are critically involved in hair follicle formation and regeneration, starts with a protein called Wnt, which is known to play an important role in maintaining and activating genes expressed during the growth of the hair cycle. Beta-catenin/TCF (T-cell factor) was measured using the TCF reporter luciferase system, which is a method for evaluating the activation of the wint/beta-catenin signaling pathway that is transferred to transcription factors.

As for human dermal papilla cells (HHFDPC), HFDPC cells cultured in the same way as in Test Example 1 were used in this experiment, and Wnt3a protein was used as a positive control to activate the wint/beta-catenin signaling pathway, and as a negative control. used DMSO. About 3x10 ⁴ of the Wnt reporter HEK293A cells were dispensed into each well in a 96-well plate, and 18 hours later, each concentration of 5 μg, 10 μg, and 20 μg for Examples 1 to 3 was treated. did Then, Wint reporter analysis was performed according to the protocol using Promega's Luciferase assay kit, and Wint/beta-catenin promoter activity was measured using PerkinElmer's luminometer Victor.

As shown in FIG. 1, the activity of the wint/beta-catenin signaling pathway was found to be almost similar to that of the untreated negative control in Example 1, whereas the wint/beta in Examples 2 and 3 were compared to the untreated negative control. -Catenin signal was amplified by 9.2-fold and 9.3-fold, respectively. In addition, the test groups of Examples 2 and 3 did not show a concentration-dependent aspect, but it was confirmed that there was a repeatable effect at a concentration of around 20 μg/mL. In particular, Examples 2 and 3 showed a slightly higher level of effect compared to the positive control Wnt3a protein (8.6 times) binding to the direct receptor of wint/beta-catenin.

Therefore, it can be confirmed that Examples 2 and 3 have an excellent hair growth promoting effect by activating the TCF transcription factor by promoting the activity of the wint/beta-catenin signaling pathway, which is a hair growth promoting signal of hair follicle stem cells.

Taken together, from the results of Test Examples 1, 2, and 3, the cacao nibs of Example 2 and the cacao mixed extract of Example 3 of the Preparation Example showed the effect of promoting proliferation of dermal papilla cells and the activity of 5-alpha-reductase. It was confirmed that the inhibitory effect and the effect of promoting the activation of the beta-catenin signaling pathway were excellent. In the present invention, when preparing a composition for preventing hair loss or promoting hair growth, cacao nibs extract or cacao bean shell, which is the endothelium of cacao beans, It was concluded that it is okay to use either extract of cacao bean extracts that have not been removed. Therefore, the composition of the formulation example below was prepared using only Example 2, which is preferably cacao nibs extract.

[Formulation Example 1] Hair Essence

In this formulation example, the hair essences of Example 2 and Comparative Example 1 were prepared in a conventional manner according to the prescriptions shown in Table 3 below.

Table 3

[Test Example 4] Safety confirmation test on human skin

In order to confirm whether the cacao extract-containing composition according to the present invention is safe for human skin when used, a skin irritation test was performed as part of skin safety verification.

A panel of 30 men and women composed of Catholic University students was selected as the subjects of the present invention, and the hair essences of Example 2 and Comparative Example 1 in Table 3 were used for the test subjects. Specifically, after washing the back skin, the water was wiped off, and it was allowed to wait for 15 minutes in a constant temperature and humidity room (temperature 24 ± 4 ° C, relative humidity 40-45%). The same amount of the scalp essence of Example 2 was sufficiently applied to the cotton wool so that it did not flow, and it was attached to the position of the skin on the back. After 24 hours and 48 hours after attaching the test substance, the subject indicated the degree of stimulation from 0 to 4 points (0 = no stimulation, 1 = very weak, 2 = weak, 3 = moderate, 4 = severe). (measured twice in total), stimuli such as stinging and burning were evaluated.

Table 4 below shows the number of subjects who complained of irritation according to the rating for each time period, and the average value was calculated to derive a mean score for skin irritation.

table 4

From the results in Table 4, the hair essence composition containing 10% of Example 2 showed no skin irritation, the same as Comparative Example 1 not containing the cacao nibs extract.

Therefore, it was confirmed that the composition according to the present invention using the cacao nibs extract was also safe for the human body, did not irritate the scalp, and had no side effects even when used for a long period of time.

[Formulation Example 2] Hair Tonic

In this formulation example, the hair tonic of Examples 2-1 to 2-5 and Comparative Example 2 according to the content of the composition of Example 2 was 0.1, 0.5, 1.0, 2,.0 and 5.0% by weight, the prescription shown in Table 5 below It was prepared by the usual method according to.

table 5

[Test Example 5] Confirmation of the effect of promoting hair growth

From the results of Test Examples 1, 2, and 3, the effect of promoting the proliferation of dermal papilla cells, the effect of inhibiting the activity of 5-alpha-reductase, and the effect of promoting the activation of beta-catenin signaling pathway in cacao nibs extract was remarkably excellent. As confirmed, in the present invention, the hair growth stimulating effect test was conducted on the hair tonic composition of Example 2 as cacao extract.

For the human body application test, the Ministry of Food and Drug Safety's "Guidelines for human application test of cosmetics that help alleviate hair loss symptoms" were applied, and the test subjects for the present invention were found to have significantly less hair than normal or hair loss through a questionnaire. A total of 60 men and women in their 20s to 50s who were recognized to have symptoms were divided into two groups consisting of 50 experimental groups and 10 non-control groups, and the purpose and purpose of the present invention was explained to all of them, and After receiving the consent to participate in the application experiment, an experiment to verify the effect of the present invention on the improvement of hair growth was conducted centering on the subjects.

Specifically, the test subject was a panel consisting of 60 adult men and women, 10 students and 50 of their direct descendants, consisting of Catholic University students and their direct descendants, and the experimental group was the hair tonic composition of Formulation Example 2, the above Examples 2-1 to 2- A total of 50 people were selected by dividing into 5 groups according to 5, and 10 people each were selected, and the remaining 10 people were selected as a control group. In order to confirm the hair growth promoting effect, Examples 2-1 to 2-5 and Comparative Example 2 were used, and were used for hair and scalp 7 times a week for 3 months.

After use, it was evaluated by dividing it into three stages according to the degree of improvement or damage as an evaluation criterion for four evaluation items such as thickness of hair, degree of denseness, volume and elasticity, respectively, and for each of Examples 2-1 to 2-5 An overall evaluation was judged (degree of improvement: +5, +4, +3, +2, +1, no improvement: 0, degree of impairment: -1, -2, -3, -4, -5). After 3 months from the start of use, the evaluation of the thickness and density of hair was carried out using a hair diagnostic device IS-3000C (Besutopia, Korea) to measure the change in hair diameter and number of hairs in the phototrichogram And, together with the results, the evaluation of the thickness, density, volume and elasticity of the hair was added together with the results of the user questionnaire in the form of a questionnaire survey, and the overall evaluation was calculated and determined.

table 6

From the results of Table 6, it was evaluated that the hair growth promoting effect was very high in Examples 2-3 to 2-5 compared to Comparative Example 2 containing no cacao nibs extract.

Therefore, it was confirmed that the cacao extract can be effectively used for hair growth promotion when preparing a hair tonic composition of 1% or more.

In summary, by containing the cacao extract of the present invention as an active ingredient, it promotes hair growth by inducing the proliferation of dermal papilla cells and inhibits the activity of 5 alpha-reductase to inhibit the production of dihydrotestosterone as well as wint/ It was confirmed that the effect of promoting the activity of the beta-catenin signaling pathway was excellent.

Having described specific parts of the present invention in detail above, it is clear that these specific techniques are only preferred embodiments for those skilled in the art, and the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Hereinafter, formulation examples of cosmetic, pharmaceutical and food compositions for preventing hair loss and promoting hair growth containing the cacao nibs extract according to the present invention are presented, which are the following formulation examples for the cosmetic and pharmaceutical compositions for preventing hair loss and promoting hair growth It is not intended to be limiting, but merely intended to be specific.

[Formulation Example 3] Cosmetic formulation

[Formulation Example 3-1] Hair shampoo

Hair shampoo was prepared in a conventional manner according to the composition shown in Table 7 below.

table 7

[Formulation Example 3-2] Hair Serum

Hair serum was prepared in a conventional manner according to the composition shown in Table 8 below.

Table 8

[Formulation Example 3-3] Hair nutrient lotion

According to the composition shown in Table 9 below, a hair nourishing lotion was prepared in a conventional manner.

Table 9

[Formulation Example 4] Pharmaceutical formulation

[Formulation Example 4-1] Preparation of powder

Powders were prepared by a conventional method according to the compositions shown in Table 10 below.

Table 10

[Formulation Example 4-2] Preparation of capsule formulation

According to the composition shown in Table 11 below, capsules were prepared by filling gelatin capsules in a conventional manner.

Table 11

[Formulation Example 4-3] Preparation of ointment

An ointment was prepared by a conventional method according to the composition shown in Table 12 below.

Table 12

[Formulation Example 5] Food formulation

[Formulation Example 5-1] Production of Drinking Agent

After mixing 80 mg of cacao nibs extract, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide, 300 ml of purified water was added and each bottle was filled to 200 ml. After filling the bottle, the beverage was prepared by sterilizing at 130 ° C. for 4 to 5 seconds.

[Formulation Example 5-2] Preparation of granules

80 mg of cacao nibs extract, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose, and 550 mg of starch were mixed, molded into granules using a fluid bed granulator, and then filled into bags.

[Prior art literature]

[Patent Literature]

(Patent Document 0001) Republic of Korea Patent Registration No. 10-1902988

(Patent Document 0002) Republic of Korea Patent Registration No. 10-0973782

Claims (5)

A composition for preventing hair loss or promoting hair growth comprising cacao extract as an active ingredient. According to claim 1,
The composition promotes hair growth by inducing the proliferation of dermal papilla cells, inhibits the activity of 5-alpha-reductase to inhibit the production of dihydrotestosterone, and promotes the activity of the wint/beta-catenin signaling pathway. A composition for preventing hair loss or promoting hair growth. According to claim 1,
The composition is a composition for preventing hair loss or promoting hair growth, characterized in that for increasing the thickness, density, volume and elasticity of the hair. According to claim 1,
The content of the cacao extract is 0.001 to 30.0% by weight relative to the total weight of the composition, characterized in that the composition for preventing hair loss or promoting hair growth. According to any one of claims 1 to 4,
The composition is a composition for preventing hair loss or promoting hair growth, characterized in that the cosmetic composition, pharmaceutical composition and food composition.

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