KR102625769B1 - A composition for preventing, treating or improving hair loss comprising Rhynchosia Nulubilis extract and Polygonum Multiflorum extract and use thereof - Google Patents

Abstract

The present invention relates to a composition for preventing, treating, alleviating, or promoting hair loss, and uses thereof, containing a Rhynchosia nulubilis extract and a Polygonum multiflorum extract as active components, and uses thereof. According to one aspect, it has been found that the composition comprising a mixed extract of Rhynchosia nulubilis and Polygonum multiflorum of the present invention can effectively enhance hair follicle cell proliferation, especially in the presence of the male hormone testosterone, and the composition can be used for the treatment or prevention of hair loss.

Description

A composition for preventing, treating or improving hair loss comprising Rhynchosia Nulubilis extract and Polygonum Multiflorum extract and use thereof}

The present invention relates to a composition for preventing, treating, improving, or promoting hair growth containing medicinal soybean extract and red water extract as active ingredients, and its use.

Hair loss refers to an abnormal increase in the number of hairs falling out during the growth cycle of hair follicles, as the ratio of hair growing in hair follicles in the catagen or telogen phase increases compared to hair growing in hair follicles in the growth phase. The causes of such hair loss are discussed as poor blood circulation, excessive male hormone action, excessive sebum secretion, decreased scalp function due to peroxides and bacteria, genetic factors, aging, and stress. In general, the most typical form of hair loss is male-pattern alopecia, which is caused by excessive action of male hormones and occurs dependent on androgens, male hormones. Representative androgens include testosterone (testosterone) and DHT (dihydrotestosterone).

Drugs approved by the U.S. FDA for hair loss treatment include minoxidil and Propecia (Finasteride), but Propecia has typical side effects such as erectile dysfunction and decreased libido due to its mechanism of suppressing male hormones, and it cannot be used for women of childbearing age. There is. In addition, minoxidil preparations such as Rogaine are known to cause side effects such as erythema, heart palpitations, and arrhythmia with prolonged use. In fact, the hair loss treatment effect of minoxidil is very low, at about 30% of the patients to whom it is applied. Therefore, various studies are being conducted on raw materials to effectively treat hair loss diseases without skin irritation or hormonal side effects, with efficacy similar to or higher than that of existing hair loss treatments. In particular, research on hair loss improvement functional cosmetics using various natural product-derived substances is being conducted. It is being done actively.

Under this background, a mixed extract was prepared by mixing the Yakbean extract and the Red Water extract at a specific ratio, and the excellent hair loss treatment and hair growth promotion effects of the mixed extract were confirmed to complete the present invention.

Republic of Korea Patent No. 10-2216651

One aspect provides a pharmaceutical composition for preventing or treating hair loss, comprising as active ingredients a medicinal bean extract and a red bean extract.

Another aspect provides a food composition for improving hair loss or promoting hair growth, comprising as active ingredients a soybean extract and a red bean extract.

Another aspect provides a feed composition for improving hair loss or promoting hair growth, comprising a medicinal bean extract and an extract of red bean sprouts as active ingredients.

Another aspect provides a cosmetic composition for improving hair loss or promoting hair growth, which includes a medicinal bean extract and a red bean extract as active ingredients.

One aspect is to provide a pharmaceutical composition for preventing or treating hair loss, comprising as active ingredients a medicinal bean extract and a red bean extract.

As used herein, the term "Rhynchosia Molubilis" refers to small-grained black beans that have black skin and are smaller than regular black beans. There are soybeans, soybeans, medicinal soybeans, Dawon soybeans, and soybeans No. 2. Although they are much smaller than ordinary black beans, they contain much more genistein and daidzein, which are useful isoflavones, than other black beans. It is done.

The term "Polygonum Multiflorum" in this specification refers to a perennial vine grass, and the vines grow up to 3 to 4 m in length by entangling each other. The rhizome is hard and woody and extends sideways, with tubers of various shapes forming at the end. It is called Jeokhasuo because of the reddish appearance of the stem with tuberous root bark and vertical lines in the xylem. The tuberous roots of the red seaweed contain oxymethylanthrachinone derivatives, lecithin, etc., and their efficacy is to nourish the liver, kidneys, and blood, strengthen tendons and bones, relieve fatigue in the lower back and knees due to kidney weakness, and improve blood flow. It is known to be used for cervical lymph node tuberculosis, carbuncle poisoning, hemorrhoids, and postpartum disease, as it causes blockage of stool due to lack of juice.

The term "extract" in this specification refers to the result, such as a liquid component obtained by immersing the desired material in various solvents and then extracting it at room temperature or at a heated state for a certain period of time, and a solid component obtained by removing the solvent from the liquid component. It can mean. In addition, in addition to the above results, it is to be comprehensively interpreted to include extracts of all formulations that can be formed using the extract itself and the extract, such as dilutions of the results, concentrates, crude preparations, purifications, or mixtures thereof. You can.

The extract may be extracted from a natural, hybrid, or variant plant of the corresponding plant, and may also be extracted from a plant tissue culture.

The extraction solvent for the extract may be a protic polar solvent or an aprotic polar and non-polar solvent. The protic polar solvent may be water, methanol, ethanol, propanol, isopropanol, or butanol. The aprotic polar solvent may be dichloromethane, tetrahydrofuran, ethyl acetate, acetonitrile, dimethylformamide, dimethyl sulfoxide, acetone, 2-butanone, or hexamethylphosphoramide. The non-polar solvent may be pentane, hexane, chloroform, or diethyl ether. The non-polar solvent excludes benzene. The solvent may be a C1-C6 alcohol, a C3-C10 ester, such as a C3-C10 acetate, a C3-C10 ketone, a C1-C6 unsubstituted or halogenated hydrocarbon, a C2-C10 ring ether, or mixtures thereof. or it may be a mixture of one or more of the above solvents and water. The solvent may be ethanol, propanol, acetonitrile, ethyl acetate, acetone, 2-butanone, chloroform, dichloromethane, hexane, mixtures thereof, or a mixture of one or more of the above solvents and water. The hydrocarbon may be an alkane, alkene, or alkyne.

The extract is water; C1 to C4 lower alcohols such as methanol, ethanol, propanol, and butanol; Polyhydric alcohols such as glycerine, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, bezene, hexane, diethyl ether, and dichloromethane. It may be extracted with one or more solvents selected from the group. Specifically, the extract may be extracted with water and/or ethanol as a solvent.

The extract may be extracted by one or more methods selected from the group consisting of reduced pressure high temperature extraction, boiling water extraction, reflux extraction, hot water extraction, cold immersion extraction, room temperature extraction, ultrasonic extraction, steam extraction, and fractional extraction.

As used herein, the term “fraction” refers to a result obtained by performing fractionation to separate a specific component or specific group of components from a mixture containing various components.

The fractionation method for obtaining the above fraction is not particularly limited and may be performed according to methods commonly used in the art. Non-limiting examples of the fractionation method include fractionation performed by treating various solvents, ultrafiltration fractionation performed by passing through an ultrafiltration membrane with a certain molecular weight cut-off value, and various chromatography methods (size, charge, hydrophobicity or There are chromatographic fractionation methods (designed for separation based on affinity), and combinations thereof.

The type of fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fractionating solvent include polar solvents such as water and alcohols having 1 to 4 carbon atoms; Non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane; Or a mixed solvent thereof, etc. may be mentioned. These may be used alone or in combination of one or more types, but are not limited thereto.

The composition may include the medicinal bean extract and the red bean extract at a concentration ratio of 1:1 to 16:1, specifically, 1:1 to 16:1, 1:1 to 14:1, and 1:1 to 1:1. 12:1, 1:1 to 10:1, 1:1 to 8:1, 1:1 to 7:1, 1:1 to 6:1, 1:1 to 5:1 1:1 to 4:1 , 2:1 to 16:1, 2:1 to 14:1, 2:1 to 12:1, 2:1 to 10:1, 2:1 to 8:1, 2:1 to 7:1, 2 :1 to 6:1, 2:1 to 5:1 2:1 to 4:1, 3:1 to 16:1, 3:1 to 14:1, 3:1 to 12:1, 3:1 to 10:1, 3:1 to 8:1, 3:1 to 7:1, 3:1 to 6:1, 3:1 to 5:1 3:1 to 4:1, 4:1 to 16:1 , 4:1 to 14:1, 4:1 to 12:1, 4:1 to 10:1, 4:1 to 8:1, 4:1 to 7:1, 4:1 to 6:1 or 4 It may be included at a concentration ratio of :1 to 5:1.

The composition may contain the Yakbean extract at a concentration of 1 to 100 ug/mL, specifically, 1 to 100 ug/mL, 1 to 80 ug/mL, 1 to 60 ug/mL, and 1 to 40 ug/mL. , 1 to 20 ug/mL, 5 to 100 ug/mL, 5 to 80 ug/mL, 5 to 60 ug/mL, 5 to 40 ug/mL, 5 to 20 ug/mL, 10 to 100 ug/mL, 10 to 80 ug/mL, 10 to 60 ug/mL, 10 to 40 ug/mL, 10 to 20 ug/mL, 15 to 100 ug/mL, 15 to 80 ug/mL, 15 to 60 ug/mL, 15 It may be included at a concentration of from 40 ug/mL, 15 to 20 ug/mL, 20 to 100 ug/mL, 20 to 80 ug/mL, 20 to 60 ug/mL, or 20 to 40 ug/mL.

The composition may contain a dripping sewage extract at a concentration of 1 to 50 ug/mL, specifically, 1 to 50 ug/mL, 1 to 40 ug/mL, 1 to 30 ug/mL, and 1 to 20 ug/mL. mL, 1 to 10 ug/mL, 5 to 50 ug/mL, 5 to 40 ug/mL, 5 to 30 ug/mL, 5 to 20 ug/mL, 5 to 10 ug/mL, 10 to 50 ug/mL , it may be included at a concentration of 10 to 40 ug/mL, 10 to 30 ug/mL, or 10 to 20 ug/mL.

The composition may be prepared by mixing the medicinal bean extract and the dripping water extract or/or mixing the medicinal bean extract and the dripping water extract and then extracting them by the method described above.

The term “alopecia” as used herein refers to a condition in which hair disappears from areas where hair should normally exist. Hair goes through a hair cycle consisting of a growth phase (anagen), a catagen phase, and a resting phase (telogen). More specifically, the growth phase, in which keratinocytes stimulated by the dermal papilla actively divide and proliferate and hair grows; the catagen phase, in which the blood supply to the hair bulb is cut off and the dermal papilla separates from the hair follicle; and cell proliferation. It stops and goes through a telogen phase, in which hair does not grow, and then goes into a growth phase again, or a shedding phase (exogen), in which hair falls out from the scalp. Each human hair has an independent growth cycle, with some entering the shedding phase and some entering the growth phase, maintaining the same level of hair count overall. However, in the case of hair loss, this balance tilts toward the shedding phase, causing hair to gradually disappear. The causes of hair loss are diverse, and largely include internal factors such as male hormone degeneration, autoimmunity, endocrine disease, childbirth, menopause, and aging, and external factors such as nutritional deficiencies, drugs, stress, scalp contamination, scalp dryness, and Demodex. there is.

The hair loss may include one or more selected from the group consisting of alopecia areata, male pattern baldness, female pattern baldness, neuropathic alopecia, pityriasis alopecia, seborrheic alopecia, pre-age alopecia, senile alopecia, and postpartum alopecia, which are now limited. no.

The hair loss may be hair loss caused by testosterone, and specifically, it may be male pattern hair loss caused by testosterone, a male hormone.

The composition may exhibit one or more characteristics selected from the group consisting of promoting the activity of dermal papilla cells, promoting proliferation of dermal papilla cells, and inhibiting death of dermal papilla cells. Specifically, the dermal papilla cells may be cells damaged by testosterone.

The term “treatment” as used herein refers to any action that improves or beneficially changes the symptoms of a hair loss disease by administering the composition of the present invention.

The term “prevention” as used herein refers to any action that suppresses or delays the possibility of developing a hair loss disease or disease by administering the composition of the present invention.

The pharmaceutical composition may include a pharmaceutically acceptable carrier. The “pharmaceutically acceptable carrier” may mean a carrier or diluent that does not irritate living organisms and does not inhibit the biological activity and properties of the injected compound. Here, “pharmaceutically acceptable” means that it does not inhibit the activity of the active ingredient and does not have any toxicity beyond what the subject of application (prescription) can adapt to. The type of carrier that can be used in the pharmaceutical composition can be any carrier that is commonly used in the art and is pharmaceutically acceptable. Non-limiting examples of the carrier include lactose, dextrose, maltodextrin, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, glycerol, ethanol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or Mineral oil, etc. may be mentioned. These may be used individually or in combination of two or more types. The pharmaceutical composition may be prepared into an oral formulation or a parenteral formulation depending on the route of administration by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. The pharmaceutical composition can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions according to conventional methods.

When formulating the pharmaceutical composition, it may be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, or surfactants, but may not be limited thereto.

When the pharmaceutical composition is prepared as an oral dosage form, it can be prepared in the form of powder, granules, tablets, pills, sugar-coated tablets, capsules, solutions, gels, syrups, suspensions, wafers, etc., along with a suitable carrier, according to methods known in the art. It can be prepared as a dosage form. At this time, examples of suitable pharmaceutically acceptable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, and wheat starch, cellulose, methylcellulose, and ethylcellulose. Cellulose such as sodium carboxymethylcellulose and hydroxypropylmethylcellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable. Yu, etc. can be mentioned. In the case of formulation, if necessary, diluents and/or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants may be included in the formulation.

When the pharmaceutical composition is prepared as a parenteral formulation, it can be formulated in the form of injections, transdermal administration, nasal inhalation, and suppositories along with a suitable carrier according to methods known in the art. When formulated as an injection, suitable carriers include sterile water, ethanol, polyols such as glycerol or propylene glycol, or mixtures thereof, preferably Ringer's solution, phosphate buffered saline (PBS) containing triethanol amine, or sterile injectable solution. Isotonic solutions such as water or 5% dextrose can be used. When formulated for transdermal administration, it can be formulated in the form of ointments, creams, lotions, gels, external solutions, paste preparations, linear preparations, and aerol preparations. In the case of nasal inhalation, it can be formulated in the form of an aerosol spray using suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, and carbon dioxide. When formulated as a suppository, the base is Wethepsol ( witepsol), Tween 61, polyethylene glycols, cocoa fat, laurel paper, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, sorbitan fatty acid esters, etc. can be used.

The pharmaceutical composition may be administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount" means an amount sufficient to treat or prevent a disease with a reasonable benefit/risk ratio applicable to medical treatment or prevention, and the effective dose level is determined by the severity of the disease, the activity of the drug, and the patient's Factors including age, weight, health, gender, patient's sensitivity to drugs, administration time of the composition of the present invention used, administration route and excretion rate, treatment period, drugs mixed or used simultaneously with the composition of the present invention used, and other factors. It can be determined based on factors well known in the medical field. The pharmaceutical composition of the present invention can be administered alone or in combination with ingredients known to exhibit therapeutic effects on known hair loss diseases. It is important to consider all of the above factors and administer the amount that will achieve the maximum effect with the minimum amount without side effects.

The dosage of the pharmaceutical composition can be determined by a person skilled in the art considering the purpose of use, the degree of addiction of the disease, the patient's age, weight, gender, antecedent history, or the type of substance used as an active ingredient. For example, the pharmaceutical composition of the present invention can be administered at about 0.1 ng to about 1,000 mg/kg, preferably 1 ng to about 100 mg/kg per adult, and the frequency of administration of the composition of the present invention is specifically determined by this. Although not limited, it can be administered once a day, or the dose can be divided and administered several times. The above dosage or frequency of administration does not limit the scope of the present application in any way.

Another aspect is to provide a method for treating or preventing hair loss comprising administering the pharmaceutical composition for preventing or treating hair loss to an individual. The same parts as described above also apply to the above method.

The term "individual" used herein may include, without limitation, mammals, birds, reptiles, farmed fish, etc., including dogs, cats, rats, livestock, humans, etc., that develop or are at risk of developing hair loss disease. , the entity may exclude humans.

The pharmaceutical composition may be administered singly or multiple times in a pharmaceutically effective amount. At this time, the composition can be formulated and administered in the form of a solution, powder, aerosol, injection, infusion solution (injection), capsule, pill, tablet, suppository, or patch. The administration route of the pharmaceutical composition for preventing or treating hair loss disease can be administered through any general route as long as it can reach the target tissue.

The pharmaceutical composition is not particularly limited, but depending on the purpose, intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, transdermal patch administration, oral administration, intranasal administration, intrapulmonary administration, intrarectal administration, etc. It can be administered through the route. However, during oral administration, it can be administered in an unformulated form, and since the active ingredients of the pharmaceutical composition may be denatured or decomposed by stomach acid, the oral composition is formulated to coat the active agent or protect it from decomposition in the stomach. It can also be administered orally in the form of a tablet or oral patch. Additionally, the composition can be administered by any device that allows the active substance to move to target cells.

Another aspect is to provide a food composition for improving hair loss or promoting hair growth, which includes a medicinal bean extract and a red bean extract as an active ingredient. The same parts as described above also apply to the composition.

The term “improvement” as used herein refers to any action that improves or beneficially changes the symptoms of a hair loss disease by administering the composition of the present invention.

The term “hair growth promotion” or “hair growth promotion” as used herein may mean any action that increases hair growth from hair follicles, for example, proliferation of hair follicle cells, activity of hair follicle cells, or growth of hair follicle cells. It can refer to any action that increases the total amount of hair, such as the effect of promoting hair.

In the present specification, the terms “hair loss improvement” or “hair growth promotion” may be used with the same meaning, and have the same meaning as another term for hair growth or hair growth promotion used in the art.

The term "food" in this specification refers to meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, and alcoholic beverages. , vitamin complexes, health functional foods, and health foods, etc., and include all foods in the conventional sense.

The food can be manufactured by methods commonly used in the industry, and can be manufactured by adding raw materials and ingredients commonly added in the industry. Additionally, the food formulation can be manufactured without limitation as long as it is a formulation recognized as a food. The food composition can be manufactured in various types of formulations, and unlike general drugs, it is made from food as a raw material, has the advantage of not having side effects that may occur when taking the drug for a long time, and is highly portable, so the food composition of the present invention It can be taken as a supplement to improve the effect of hair loss.

The food composition may be a health functional food composition.

The term “health functional food” in this specification refers to food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with Act No. 6727 on Health Functional Food, and “functional” refers to food that is manufactured and processed using functional ingredients useful to the human body. It means controlling the structure and function of nutrients or obtaining useful effects for health purposes such as physiological effects.

The above-mentioned health food refers to food that has a more active health maintenance or promotion effect compared to general food, and health supplement food refers to food for the purpose of supporting health. In some cases, the terms health functional food, health food, and health supplement may be used interchangeably. Specifically, the health functional food is a food manufactured by adding the composition to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspending it, and has specific health effects when consumed. However, unlike regular drugs, it has the advantage of being made from food and eliminating the side effects that can occur when taking the drug for a long period of time.

Since the food composition can be consumed on a daily basis, it can be expected to be highly effective in improving hair loss diseases, so it can be very useful.

The food composition may further include a physiologically acceptable carrier. The type of carrier is not particularly limited and any carrier commonly used in the art can be used. Specifically, the food composition may include additional ingredients that are commonly used in food compositions to improve smell, taste, vision, etc. For example, it may include vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, etc. Additionally, it may contain minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr). Additionally, it may contain amino acids such as lysine, tryptophan, cysteine, and valine.

In addition, the food composition contains preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching powder, high bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisole (BHA), butylhydroxide) roxitoluene (BHT), etc.), colorants (tar color, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleaching agents (sodium sulfite), seasonings (MSG monosodium glutamate, etc.), sweeteners (dulcine, cyclemate, saccharin) , sodium, etc.), flavorings (vanillin, lactones, etc.), leavening agents (alum, D-potassium hydrogen tartrate, etc.), strengtheners, emulsifiers, thickeners (grease), coating agents, gum base agents, anti-foam agents, solvents, improvers, etc. May contain food additives. The additives can be selected depending on the type of food and used in an appropriate amount.

The food composition can be added as is or used together with other foods or food ingredients, and can be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment). In general, when producing a food or beverage, the food composition of the present invention may be added in an amount of 50 parts by weight or less, specifically 20 parts by weight or less, relative to the food or beverage. However, when consumed for a long time for health and hygiene purposes, the content may be below the above range. Since there is no problem in terms of safety, the active ingredient may be used in amounts above the above range.

As an example of the food composition, it can be used as a health drink composition, and in this case, it may contain various flavoring agents or natural carbohydrates as additional ingredients like regular drinks. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; polysaccharides such as dextrins and cyclodextrins; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as thaumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame can be used. The ratio of the natural carbohydrate may be generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g, per 100 mL of the health drink composition of the present invention.

In addition to the above, the health drink composition includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol or carbonating agent. Additionally, it may contain pulp for the production of natural fruit juice, fruit juice beverage, or vegetable beverage. These ingredients can be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health drink composition of the present invention.

The food composition may contain the extract of the present invention in various weight% if it can exhibit an effect of improving or preventing hair loss disease. Specifically, the extract may be added in an amount of 0.00005 to 100% by weight or 0.05 to 80% by weight based on the total weight of the food composition. It may be included as a %, but is not limited thereto.

Another aspect is to provide a feed composition for improving hair loss or promoting hair growth, which includes medicinal bean extract and red water extract as active ingredients. The same parts as described above also apply to the composition.

The term “feed” in this specification may mean any natural or artificial diet, meal, etc., or a component of the meal, for or suitable for eating, ingestion, and digestion by animals.

The type of feed is not particularly limited, and feed commonly used in the art can be used. Non-limiting examples of the feed include plant feeds such as grains, roots and fruits, food processing by-products, algae, fiber, pharmaceutical by-products, oils and fats, starches, cucurbits or grain by-products; Examples include animal feeds such as proteins, inorganic substances, fats and oils, minerals, oils and fats, single-cell proteins, zooplanktons or food. These may be used individually or in combination of two or more types.

The feed composition may further include known additives that can be added to improve or prevent hair loss diseases depending on the formulation. The feed composition may be in the form of a highly concentrated liquid, powder, or granule. The feed composition may further include any protein-containing organic cereal flour commonly used to meet the dietary needs of animals. The feed composition can be used by adding it to animal feed by dipping, spraying, or mixing.

The feed composition may additionally contain substances that have various effects, such as nutrient supplementation and weight loss prevention, improvement of digestibility of fiber in feed, improvement of milk quality, prevention of reproductive disorders and improvement of conception rate, and prevention of high temperature stress in summer. For example, mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, complex minerals, trace mineral preparations such as zinc, copper, cobalt, and selenium, kerotene, vitamin E, vitamins A, D, E, and nicotinic acid. , vitamins such as vitamin B complex, protective amino acids such as methionine and lysic acid, protective fatty acids such as fatty acid calcium salts, probiotics (lactic acid bacteria), yeast cultures, live bacteria such as mold fermentation products, yeast agents, etc. may be included.

The feed composition can be applied to a number of animal diets, including mammals and poultry, that is, feed and drinking water.

The feed composition may include all substances added to the feed (i.e., feed additives), feed raw materials, or the feed itself fed to the individual.

Another aspect is to provide a cosmetic composition for improving hair loss or promoting hair growth, which includes medicinal bean extract and red water extract as active ingredients. The same parts as described above also apply to the composition.

The formulation of the cosmetic composition is not limited as long as it is a typical cosmetic formulation, but for example, skin lotion, astringent lotion, or nourishing lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing cream, etc. It may be water, pack, powder, body lotion, body cream, body oil, or body essence.

The cosmetic composition may contain substances arbitrarily selected according to the formulation or purpose of use of the cosmetic, for example, preservatives, stabilizers, surfactants, solubilizers, moisturizers, emollients, ultraviolet absorbers, preservatives, disinfectants, antioxidants, pH It may further include conditioners, organic and inorganic pigments, fragrances, cooling agents or antiperspirants. The mixing amount of the above additional ingredients can be easily selected by a person skilled in the art within a range that does not impair the purpose and effect of the present invention.

When the formulation of the cosmetic composition is a paste, cream or gel, the carrier component may include, for example, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. It may include etc.

When the formulation of the cosmetic composition is a powder or spray, the carrier component may include, for example, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder. In particular, in the case of a spray, chlorofluorocarbons may be added. May contain propellants such as lohydrocarbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, the carrier component may include, for example, a solvent, a solubilizing agent, or an emulsifying agent, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, and benzyl alcohol. , benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition is a suspension, the carrier component may include, for example, water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. It may include microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant.

When the formulation of the cosmetic composition is a surfactant-containing cleansing agent, the carrier component may include, for example, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosylate. It may include nitrate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

In one embodiment, the cosmetic composition is a skin external formulation and includes hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, It may have one or more formulations selected from the group consisting of eyebrow hair growth agents, eyelash hair growth agents, and eyelash nutrients.

Another aspect is to provide a method for improving hair loss or promoting hair growth, comprising applying the cosmetic composition for improving hair loss or promoting hair growth to an individual. The same parts as described above also apply to the above method.

The method may be a cosmetic method by improving hair loss or promoting hair growth.

The term “application” as used herein includes methods of administration or application to the skin of a subject, such as application by fingers, brush, cotton ball, pad, spray, sponge, cotton swab, roll-on, etc. . A person skilled in the art can easily determine the appropriate method to apply.

In the method, the applying step may be applying the composition at intervals of 1 minute to 90 minutes, specifically 1 minute to 90 minutes, 5 minutes to 90 minutes, 1 minute to 60 minutes, and 5 minutes to 90 minutes. It may be at intervals of 60 minutes, 1 minute to 30 minutes, 5 minutes to 30 minutes, 10 minutes to 30 minutes, 10 minutes to 90 minutes, or 10 minutes to 60 minutes.

In the method, the applying step may be applying the composition 1 to 30 times, specifically 1 to 30 times, 1 to 25 times, 5 to 30 times, 5 to 25 times, It may be applied 1 to 20 times, 5 to 20 times, 10 to 20 times, 10 to 30 times, or 10 to 25 times.

According to one aspect, it was confirmed that the composition containing the mixed extract of medicinal bean and red water of the present invention can effectively improve the proliferative ability of dermal papilla cells, and in particular, can effectively proliferate dermal papilla cells even in the presence of testosterone, a male hormone. , the composition can be used for hair loss treatment or prevention.

Figure 1 is a diagram showing the proliferative ability of Yakkong extract on dermal papilla cells.
Figure 2 is a diagram showing the proliferative ability of the extract of Red Sage extract on dermal papilla cells.
Figure 3 is a diagram showing the proliferative ability of the mixed extract of Yakkong and Jeokhasoo on testosterone-treated dermal papilla cells.

This will be described in more detail through examples below. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1: Preparation of mixed extract of medicinal soybean and red sewage water

In order to prepare a mixed extract containing Yakbean extract and Jeokshu extract, the following experiment was performed.

Specifically, 70% alcohol (70% ethanol) extraction was performed for 15 hours at 31.5°C for each of Yakkong or Red Water. The extract was freeze-dried and powdered. Afterwards, in the following examples, the extracts of the medicinal soybean or red water extract were used independently or mixed at a concentration ratio of 1:1 to 8:1 and used in the experiment.

Example 2: Evaluation of dermal papilla cell proliferative ability of medicinal bean extract or red water extract

In order to evaluate the hair papilla cell proliferative ability of each of the Yakkong extract or the Jeokhasu extract prepared in the above example using the MTT assay, the following experiment was performed.

Specifically, dermal papilla cells were seeded in a 96-well plate, and DMEM medium containing 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin was used under conditions of 5% CO ₂ and 37°C. and cultured for 24 hours. Afterwards, the medium was replaced with serum-free DMEM and serum starvation conditions were performed. Next, the medicinal bean extract or the dripping water extract prepared in Example 1 was treated for 72 hours at various concentrations (yakkong: 2.5 to 40 μg/mL, dripping water: 2.5 to 20 μg/mL), and 5 mg/mL 110 μL of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reagent was applied per well and incubated for 1 hour. In living cells, purple formazan is formed through intracellular enzymatic reactions. After treatment with MTT reagent for 1 hour, the medium was removed, 200 μL of DMSO was added per well to dissolve the formed formazan, and the absorbance was measured at 540 nm.

As a result, it was confirmed that when the medicinal bean extract and the red bean extract were treated alone, the dermal papilla cells proliferated (Figures 1 and 2). Based on the above results, it was confirmed that the medicinal bean extract or the red bean extract had the ability to proliferate dermal papilla cells. Able to know.

Example 3: Evaluation of dermal papilla cell proliferative ability of Yakbean extract or Jeoksoo extract in the presence of testosterone

Testosterone is a hormone that plays an important role in the development and maintenance of male sexual characteristics, including the growth of facial and body hair. It is known to be associated with male pattern baldness (androgenetic alopecia), and affects the growth and proliferation of dermal papilla cells. can affect

Therefore, in the presence of testosterone, the following experiment was performed to evaluate the hair papilla cell proliferative ability of each extract of Yakkong or Jeokhasuo extract.

Specifically, dermal papilla cells were seeded in a 96-well plate, and DMEM medium containing 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin was used under conditions of 5% CO ₂ and 37°C. and cultured for 24 hours. Afterwards, the medium was replaced with serum-free DMEM and serum starvation conditions were performed. Next, the Yakkong extract or the Jeokshu extract prepared in Example 1 was pretreated for 1 hour, and then 200 uM of testosterone and extract per well were treated and cultured for 5 days. Afterwards, 110 μL per well of 5 mg/mL MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reagent was added and incubated for 1 hour. After MTT reagent treatment, the medium was removed, 200 μL of DMSO was added per well to dissolve the formed formazan, and the absorbance was measured at 540 nm. Additionally, Minoxidil, known as a hair loss treatment, was used as a positive control.

As a result, it can be seen that in the presence of testosterone, treatment with the Yakbean extract and the Red Water extract alone or with minoxidil does not induce the proliferation of dermal papilla cells (FIG. 3). Therefore, based on the above results, it can be seen that Yakkong extract or Jeokhasoo extract does not have the ability to proliferate dermal papilla cells in the presence of testosterone, which means that the dermal papilla cell proliferation ability damaged by testosterone is not restored with Yakkong extract or Jeokhasoo extract alone.

Example 4: Evaluation of dermal papilla cell proliferation ability of mixed extract of Yakkong and Jeoksoo in the presence of testosterone

In the presence of testosterone, the following experiment was performed to evaluate the hair papilla cell proliferative ability of the mixed extract of Yakkong and Jeokhasoo.

Specifically, the experiment was performed by the method described in Example 3, and instead of the Yakkong extract or the Jeonghasoo extract, the Yakbean extract and the Jeokhasoo extract were mixed and treated at a ratio of 1:1 to 8:1.

As a result, it was confirmed that, unlike Example 3, in which the extract of Yakbean or Sorghum extract was treated in the presence of testosterone, the mixed extract of Yakkong and Sorghum extract could induce the proliferation of dermal papilla cells even in the presence of testosterone (Figure 3). In particular, it was confirmed that a mixed extract in which Yakbean extract and Red Water extract were mixed at a concentration ratio of 4:1 showed excellent proliferative ability. Therefore, based on the above results, it can be seen that the mixed extract of Yakkong and Jeonghasuo has the ability to proliferate dermal papilla cells even in the presence of testosterone, which means that the proliferative ability of dermal papilla cells damaged by testosterone can be restored with the mixed extract of Yakkong and Jeokhasoo.

The description of the present invention described above is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. will be. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive.

"This patent is research conducted with the support of the National Research Foundation of Korea's Advanced Research Talent Growth Support Project, which is funded by the government (Ministry of Science and ICT) in 2023 (No. NRF-2020M3H1A1073304)."

Claims (9)

A pharmaceutical composition for preventing or treating hair loss, comprising as active ingredients a soybean extract and a dropwort extract,
The composition, wherein the medicinal bean extract and the red bean extract are included in a concentration ratio of 2:1 to 8:1. The method according to claim 1, wherein the extract is a group consisting of water, methanol, ethanol, propanol, butanol, glycerin, butylene glycol, propylene glycol, methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane. A pharmaceutical composition extracted with one or more solvents selected from. The pharmaceutical composition according to claim 1, wherein the extract is extracted by one or more methods selected from the group consisting of reduced pressure high temperature extraction, boiling water extraction, reflux extraction, hot water extraction, cold needle extraction, room temperature extraction, ultrasonic extraction, steam extraction, and fractional extraction. . The pharmaceutical composition according to claim 1, wherein the composition comprises a medicinal bean extract and a red bean extract at a concentration ratio of 4:1 to 8:1. The method of claim 1, wherein the hair loss includes at least one selected from the group consisting of alopecia areata, male pattern baldness, female pattern baldness, neuropathic alopecia, pityroid alopecia, seborrheic alopecia, pre-age alopecia, senile alopecia, and postpartum alopecia. Pharmaceutical composition. The method according to claim 1, wherein the composition is a pharmaceutical composition that represents one or more selected from the group consisting of promoting the activity of dermal papilla cells, promoting proliferation of dermal papilla cells, and inhibiting death of dermal papilla cells. A food composition for improving hair loss or promoting hair growth, comprising as active ingredients a soybean extract and a dropwort extract,
The composition, wherein the medicinal bean extract and the red bean extract are included in a concentration ratio of 2:1 to 8:1. A feed composition for improving hair loss or promoting hair growth comprising medicinal bean extract and red water extract as active ingredients,
The composition, wherein the medicinal bean extract and the red bean extract are included in a concentration ratio of 2:1 to 8:1. A cosmetic composition for improving hair loss or promoting hair growth, comprising medicinal bean extract and red water extract as active ingredients,
The composition, wherein the medicinal bean extract and the red bean extract are included in a concentration ratio of 2:1 to 8:1.