KR20220159637A - A Composition for Prevention of Hair Loss and Promotion of Hair Growth Comprising Extract of Lycopus lucidus Turcz - Google Patents

Abstract

The present invention relates to a composition for prevention of hair loss and promotion of hair growth comprising extract of Lycopus lucidus Turcz. The composition proliferates dermal papilla cells, increases VEGF expression in dermal papilla cells and secretion of IGF-1 protein in mouse blood, and increases the number of hair follicles and the thickness of skin tissue, thereby preventing hair loss or promoting hair growth.

Description

Composition for preventing hair loss and promoting hair growth containing takran extract {A Composition for Prevention of Hair Loss and Promotion of Hair Growth Comprising Extract of Lycopus lucidus Turcz}

The present invention relates to a composition for preventing hair loss and promoting hair growth comprising a takran extract.

Hair loss refers to a state in which there is no hair in an area where hair normally exists, and generally refers to the loss of terminal hair, which is thick hair on the scalp. Unlike vellus hair, which is colorless and thick, terminal hair can cause cosmetic problems if it falls out. In the case of Korea, there are about 50,000 to 70,000 hairs, and it is normal to lose about 50 to 70 hairs a day, but if more than 100 hairs are lost, it can be judged that hair loss is in progress.

There are various causes of such hair loss. In the case of androgenetic alopecia, genetic causes and androgen, the male hormone, are considered to be important factors. There is a difference. Alopecia areata is assumed to be an autoimmune disease, and telogen effluvium is temporary hair loss that occurs after severe physical and mental stress such as endocrine disease, nutritional deficiency, drug use, childbirth, fever, and surgery, and part of the hair does not complete the growth period. It has been reported that it is caused by the transition to the maintenance phase and dropout.

Human hair is primarily in charge of a defense function from external stimulants such as ultraviolet rays, but also plays an aesthetic function of expressing an individual's external image. Therefore, if hair loss progresses, self-confidence in one's appearance may decrease and sociability may decrease, and in severe cases, it may cause depression.

Currently, representative drugs approved by the FDA as drugs capable of promoting hair growth and mimic growth include minoxidil and finasteride. However, the above drugs are accompanied by side effects such as hair growth and sexual dysfunction of the whole body, and in the case of minoxidil, the specific mechanism of action is also not clearly clarified, and it is unpredictable that other side effects appear when taken for a long time. Therefore, it is necessary to develop a composition for preventing hair loss and promoting hair growth that is safe for the human body while promoting the proliferation of dermal papilla cells in hair follicles and increasing the expression of growth factors.

Republic of Korea Registration No. 10-1914744 (2018.10.29)

One aspect is to provide a food composition for preventing hair loss or promoting hair growth comprising a takran extract.

Another aspect is to provide a pharmaceutical composition for preventing hair loss or promoting hair growth comprising a takran extract.

One aspect is to provide a food composition for preventing hair loss or promoting hair growth comprising a takran extract.

The taekran is a perennial plant belonging to the Lamiaceae family, and may be a plant that grows in wetlands or streams. The taekran may also be referred to as Xipsari, Jishun, Poongyak, Horan, Yongjo, Hopo, and Rhodiola.

According to one embodiment, the takran may be collected from the aerial part of the plant before flowering or dried, but is not limited thereto.

According to one embodiment, the extract may be extracted with an extraction solvent selected from the group consisting of water, C ₁ ~ C ₄ alcohol, or a mixed solvent thereof. For example, the extract may be extracted by refluxing and stirring taekran with distilled water.

As used herein, the term 'hair loss' may belong to this without particular limitation if the number of hairs outside the normal hair growth level is less than the general number. According to one embodiment, the hair loss may be at least one selected from the group consisting of androgenetic hair loss, female pattern hair loss, circular hair loss, and telogen hair loss.

The hair may include hair roots and hair follicles of the head, hair, eyelashes, eyebrows, beard, armpits, pubic hair, and all parts of the body with hair roots and hair follicles.

As used herein, the term 'anti-hair loss' refers to any activity that inhibits or delays hair loss by administering the composition of the present invention.

As used herein, the term 'hair growth promotion' is meant to include the meaning of hair growth as well as hair growth.

The food composition may additionally contain food additives, and the suitability as a 'food additive' is determined according to the general rules and general test methods of the Food Additive Code approved by the Food and Drug Administration, unless otherwise specified. judged according to the standard.

Examples of items listed in the 'Food Additives Code' include chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as dark pigment, licorice extract, crystalline cellulose, and guar gum, L- and mixed preparations such as sodium glutamate preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations.

Foods containing the active ingredient of the present invention include bread, rice cakes, dried confections, candies, chocolates, chewing gum, confectionery products such as jams, ice creams, ice creams, ice cream products such as ice cream powders, milks, low-fat milks, lactose-degraded milks, Processed milk, goat milk, fermented milk, buttermilk, concentrated milk, milk cream, buttermilk, natural cheese, processed cheese, powdered milk, dairy products such as whey Processed meat products, processed egg products, meat products such as hamburgers Fish cakes, ham, Fish meat products such as sausages and bacon, etc. Ramen, dried noodles, raw noodles, fried noodles, deluxe dried noodles, improved deep-fried noodles, frozen noodles, noodles such as pasta, fruit drinks, vegetable drinks, carbonated drinks, soy milk , beverages such as lactobacillus beverages such as yogurt, beverages such as mixed beverages, soy sauce, soybean paste, gochujang, chunjang, cheonggukjang, mixed soy sauce, vinegar, sauces, tomato ketchup, curry, seasoning foods such as dressings, margarine, shortening, and pizza. It is not limited.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages; and the like. In addition, the composition of the present invention may include fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not critical, but is generally selected in the range of 0.01 to 0.1 part by weight per 100 parts by weight of the composition of the present invention.

Another aspect is to provide a pharmaceutical composition for preventing hair loss or promoting hair growth comprising a takran extract.

The description of the takran, taklan extract, hair loss, hair loss prevention, and hair growth promotion is the same as that described above.

In the present invention, the pharmaceutical composition may be formulated in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories or sterile injection solutions according to conventional methods. have. In detail, when formulated, it may be prepared using diluents or excipients such as commonly used fillers, weighting agents, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, and the like. Such a solid preparation may be prepared by mixing at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, gelatin, or the like, with a composition containing the fermented product of Sancheongmok or a pharmaceutically acceptable salt thereof. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized formulations and tablets. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for suppositories, Witepsol, Macrosol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like can be used.

An appropriate dosage of the composition of the present invention varies depending on the condition and body weight of the patient, the severity of the disease, the type of drug, and the time, but can be appropriately selected by those skilled in the art.

Administration of the composition containing the takran extract according to the present invention proliferates dermal papilla cells, increases VEGF expression in dermal papilla cells and IGF-1 protein secretion in mouse blood, and increases the number of hair follicles and skin tissue thickness, thereby preventing or preventing hair loss It can stimulate hair growth.

1 is data confirming the proliferation effect of dermal papilla cells.
2 is data confirming the relative expression level of VEGF, a dermal papilla cell growth factor gene.
3 is a photograph confirming the hair growth effect of the hair-removed mouse.
Figure 4 is a photograph confirming the skin tissue by cutting the skin of the mouse after hair removal.
Figure 5 is data confirming the amount of IGF-1 protein secretion in the blood of the mouse after hair removal.

Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

Example 1. Preparation of takran extract

100 g of dried taklan was pulverized and extracted with 1,000 mL of distilled water at 98 ° C. for 6 hours or more under reflux and stirring. The extracted takran hot water extract was firstly filtered through a 20 mesh vibrating sieve, then adsorbed and filtered with a second perlite to remove insoluble impurities. Thereafter, the filtered takran hot-water extract was concentrated under reduced pressure at 87 ° C using a rotary vacuum concentrator (EYELA, Tokyo, Japan), and the vacuum-concentrated hot-water extract was freeze-dried at -80 ° C (Operon, Gimpo-si, Korea) and then powdered.

Example 2. Confirmation of the proliferation effect of dermal papilla cells

In order to confirm that the takran extract is effective for hair growth, the proliferation effect of dermal papilla cells was confirmed. 5.0 × 10 ³ of dermal papilla cells in a 24-well plate After culturing in cells/well, the hot water extract of takran was treated at a concentration of 50 μg/mL and cultured for 48 hours. After culturing, the MTT assay was performed by washing twice with phosphate buffered saline. As a positive control group, commercially available medicinal yeast extract for preventing hair loss was treated with a concentration of 50 μg/mL, and in the negative control group (untreated group), positive control group, and taklan hot water extract group, 2% concentration of fetal bovine serum (Fetal Bovine Serum , FBS) was added.

As shown in Figure 1, it was confirmed that the dermal papilla cells in the group treated with the hot water extract of takran were more proliferated than the negative control group and the positive control group.

Example 3. Confirmation of dermal papilla cell growth factor gene expression promotion effect

The expression of VEGF (vascular endothelial growth factor), a growth factor gene of takran hot water extract, was confirmed. Dermal papilla cells constituting the hair root were 5 × 10 ³ in a 24-well plate. After culturing with cells/well, the hot water extract of taekran was 50 It was treated at a concentration of μg/mL and cultured for 48 hours. As a positive control group, commercially available medicinal yeast extract for preventing hair loss was treated with a concentration of 50 μg/mL, and in the negative control group (untreated group), positive control group, and taklan hot water extract group, 2% concentration of fetal bovine serum (Fetal Bovine Serum , FBS) was added. After extracting total RNA from cultured cells, cDNA was synthesized using a reverse transcription kit (Qiagen). RT-PCR was performed using Taqman gene expression master-mix (Applied Biosystems).

As shown in Figure 2, it was confirmed that the gene expression of VEGF in dermal papilla cells (HFDPC) increased more than twice as compared to the negative control group (untreated group) in the group treated with takran hot water extract.

Example 4. Confirmation of hair growth effect in mouse hair growth experiment model

All of the hair growing on the back of 7-week-old C57BL/6 female mice was removed using an epilator, and mice with skin wounds during the hair removal process were excluded from the experiment, and 5 mice were used for each treatment group. was experimented with. The takran hot water extract treatment group was provided by mixing the takran hot water extract with the general feed (AIN-93G, Raon Bio, Korea) at a concentration of 200 mg/kg. It was provided for 5 weeks, and the degree of hair growth of the hair-removed mice was observed every week. As a positive control group, a commercially available medicinal yeast extract for preventing hair loss was added to the feed at a concentration of 200 mg/kg and provided.

As shown in Figure 3, most of the mice in the untreated group did not grow hair, and the mice with hair grew only partly toward the tail, while all 5 mice in the positive control group and the takran hot water extract treatment group grew hair, among which Some confirmed that most of the hair on the back grew evenly at 5 weeks.

Example 5. Confirmation of the number of hair follicles in hair growth experiment mice

After cutting the skin of the mouse of each treatment group vertically and horizontally, H & E (Hematoxylin & Eosin) staining was performed on the cut surface, and the skin tissue was observed under a fluorescence microscope.

As shown in Figure 4, it can be seen that the number of hair follicles in the positive control group and the takran hot water extract treatment group increased significantly compared to the untreated group, and it was confirmed that the hair growth cycle in the takran hot water extract treatment group was significantly advanced compared to other groups. did

Example 6. Checking the amount of IGF-1 protein secretion in the blood of hair growth test mice

After sacrificing the 5-week-old mice, blood was collected and left at room temperature to separate serum. The amount of secreted IGF-1 protein in serum was measured using a mouse IGF-1 ELISA Kit (MG100, R&D systems, USA).

As shown in Figure 5, it was confirmed that the amount of IGF-1 protein in the blood of the takran hot water extract treatment group increased significantly compared to the untreated group, which was significantly increased than the positive control group.

Claims (4)

A food composition for preventing hair loss or promoting hair growth, comprising an extract of takran.
According to claim 1,
The extract is extracted with an extraction solvent selected from the group consisting of water, C ₁ ~ C ₄ alcohol, or a mixed solvent thereof,
A food composition for preventing hair loss or promoting hair growth.
According to claim 1,
Characterized in that the hair loss is at least one selected from the group consisting of male pattern hair loss, female pattern hair loss, circular hair loss and telogen hair loss,
A food composition for preventing hair loss or promoting hair growth.
A pharmaceutical composition for preventing hair loss or promoting hair growth comprising an extract of takran.

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