KR20140106058A - Composition for enhancing immunological activity comprising soluble salmon milt extract and vegetable lactic acid bacteria - Google Patents

Abstract

The present invention relates to a composition for enhancing immunological enhancement, comprising soluble salmon milt extract and vegetable lactic acid bacteria. Moreover, the present invention relates to a food composition and a pharmaceutical composition for preventing, improving, and treating cancer or allergy, comprising soluble salmon milt extract and vegetable lactic acid bacteria.

Description

Technical Field [0001] The present invention relates to a composition for enhancing immunity comprising a water-soluble salmon lily extract and a vegetable lactic acid bacterium.

The present invention relates to an immunoconjugate composition comprising a water-soluble salmon lily extract and a vegetable lactic acid bacterium. The present invention also relates to food compositions and pharmaceutical compositions for preventing, ameliorating or treating cancer or allergies, including water-soluble salmon extracts and vegetable lactic acid bacteria.

One of the major causes of many modern diseases that are difficult to repair, such as cancer, hypertension, obesity, arteriosclerosis, diabetes, allergies, collagen diseases, and kidney diseases, is the damage of genes due to oxygen free radicals. Active oxygen also plays a role in killing pathogenic microorganisms such as bacteria that enter the body, but it has the function of removing this active oxygen in the body because it damages its own cells or genes when it occurs in excess. In particular, harmful reactive oxygen (Oxygen) is harmful when exposed to ultraviolet rays, received electromagnetic waves from TVs, computers, microwave ovens, mobile phones, etc., harmful air such as exhaust gases, It is known that overeating occurs for various reasons such as stress. Antioxidant enzymes such as superoxide dismutase (SOD) and vitamin C present in the body play a role in inhibiting or eliminating the production of active oxygen, but they can not completely protect cells or genes from active oxygen. Nucleic acid plays an important role in healing genes damaged by free radicals. Nucleic acid is known to enhance disease immunity and stimulate metabolism to heal genes, thereby preventing disease or aging (N. Sudo et al., Clinical and Experimental Allergy, 2000, Volume 30, 979-987).

Nucleic acid is produced in the body in two ways. The first method is the nobo synthesis method in which amino acids and the like are made from the raw material. The second method is salvage synthesis using nucleic acids from foods. The total amount of nucleic acids made by synthesis of de novo and saliva is kept constant. Therefore, if the amount of nucleic acid in the food is decreased, the synthesis of denobo is increased, and when the amount of nucleic acid is increased, the synthesis of denobo is decreased. Since senescence declines at older ages, the synthesis of de novo is reduced, and if the food is not supplied with nucleic acid, it will become chronicly deficient in the body. The reduction of nucleic acid production by the de novo synthesis inhibits gene healing, so it is necessary to increase the salvage synthesis by supplying the nucleic acid from the outside.

In addition, nucleic acids made from de novo synthesis and nucleic acids made by salvage synthesis differ in terms of their use. Cancer cells are known to proliferate mainly by using nucleic acids made by denovo synthesis. Therefore, it is known that the high-nucleic acid expression decreases the rate of cancer proliferation because it increases salvage synthesis and reduces denovo synthesis.

Because nucleic acids are present in cells, it is possible to ingest nucleic acids regardless of whether they contain meat, fish, vegetables, or grains. However, the amount of nucleic acid varies greatly depending on the type of food. Foods with low protein content or refined foods contain very little nucleic acid, and sugar or cooking oil contains little or no nucleic acid. Nutrient content is not high in foods called treasures of vitamins such as vegetables and fruits. Foods that contain a relatively large amount of nucleic acids include anchovies, beef, pork, chicken, tuna, sardines, flounder, steak, tuna fish, clams, oysters, and soybeans. .

In particular, salmon roe is rich in nucleic acids and is being utilized as a material for various functional foods and cosmetics. For example, Japanese Patent Registration No. 3975448 provides a health food for the prevention of menopausal disorders comprising a nucleic acid obtained from salmon worm, and Korean Patent Publication No. 2012-0063646 discloses a method for producing a functional sap for cosmetic using salmon testes .

The present inventors have found that a composition comprising a water-soluble salmon extract and a plant lactic acid bacterium has an effect of enhancing immune function by inducing the production of interferon (INF-γ) and interleukin (IL-12), which are immunological related physiologically active substances, The present invention has been completed upon confirming that the present invention can be utilized as a medicine for health functional foods and immune function related diseases.

One object of the present invention is to provide a food composition for immunomodulation comprising a water-soluble salmon lily extract and a vegetable lactic acid bacterium.

Another object of the present invention is to provide a pharmaceutical composition for the prevention or treatment of cancer including water-soluble salmon roe extract and vegetable lactic acid bacteria.

It is another object of the present invention to provide a pharmaceutical composition for the prevention or treatment of allergy including water-soluble salmon roe extract and vegetable lactic acid bacteria.

In one aspect, the present invention relates to a food composition for enhancing immunity comprising a water-soluble salmon lily extract and a vegetable lactic acid bacterium.

In another aspect, the present invention relates to a pharmaceutical composition for preventing or treating cancer, which comprises a water-soluble salmon extract and a vegetable lactic acid bacterium.

In another aspect, the present invention relates to a pharmaceutical composition for preventing or treating allergy, which comprises a water-soluble salmon extract and a vegetable lactic acid bacterium.

Preferably, in the present invention, the vegetable lactic acid bacterium may be Pediococcus pentosaceus .

Also preferably, the composition of the present invention may be a composition further comprising yeast extract, curcuma langa extract or a combination thereof.

Preferably, the composition of the present invention further comprises at least one selected from the group consisting of yeast extract, pearl keratinocyte, vitamin C, nicotinic acid, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin B12, magnesium oxide, collagen, glucosamine, And at least one selected from the group consisting of lactic acid, turmeric extract, excipient, flavor and sweetener.

Also preferably, the composition of the present invention comprises 30-40% by weight of a water-soluble salmon extract, 0.25-0.70% by weight of a vegetable lactic acid bacterium, 10-15% by weight of a yeast extract, 0.05-0.1% by weight of a turmeric extract, Excipients, fragrances, and sweeteners.

Also preferably, the composition of the present invention has the function of improving, preventing or treating cancer or allergy through immune enhancement.

Hereinafter, the present invention will be described in more detail.

As used herein, the term "salmon roe extract" refers to an extract derived from a testis of salmon, preferably extracted from salmon roots to remove water and extract nucleic acid and protein to high purity. More preferably, the salmon roe extract may be composed of 25 to 50% by weight of nucleic acid, 25 to 60% by weight of protein and a residual water content. The nucleic acid may contain DNA or RNA, and the protein may be a basic protein containing a large amount of a basic amino acid such as arginine, preferably protamine.

As used herein, the term "water-soluble salmon roe extract" refers to an extract prepared by treating a salmon roe extract with a nuclease and / or a protease to make the nucleic acid and protein contained in the extract low-molecular- . The original salmon ginseng extract is insoluble in water because it contains a polymeric nucleic acid and a protein. However, when the water-soluble salmon ginseng extract is used as in the present invention, it can be used variously in medicine or foodstuffs such as a drink, Can be used effectively. However, if the nucleic acid and the protein are reduced to low molecular weight without limitation, the original nucleic acid and the protein may lose the effect. Therefore, the low molecular weight nucleic acid and the protein contained in the water-soluble salmon extract of the present invention have a molecular weight of 1,000 to 3,000 .

In the present application, the water-soluble salmon extract can be produced using conventional methods known in the art. For example, after removing the skin, blood vessels, blood vessels, etc. from saliva, the smell can be removed by dipping in water and washing with water. The salmonid gland extract can then be prepared by comminuting salmon gland with water and treating with nuclease and / or protease.

In addition, the composition of the present invention may further comprise yeast extract, turmeric extract, or a combination thereof for the purpose of further enhancing the immune function or for imparting desirable characteristics of the form. More preferably, yeast extract, pearl keratin calcium, vitamin C, nicotinic acid, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin B12, magnesium oxide, collagen, glucosamine, shark cartilage, hyaluronic acid, , An excipient, a flavoring agent, and a sweetener.

Preferably, the composition of the present invention comprises 25-45% by weight of a water-soluble salmon extract, 0.15-0.80% by weight of a vegetable lactic acid bacterium, 7.5-17.5% by weight of a yeast extract, 0.01-0.15% by weight of a turmeric extract, , Fragrances, and sweeteners. More preferably, the composition contains 30 to 40% by weight of a water-soluble salmon extract, 0.25 to 0.70% by weight of a vegetable lactic acid bacterium, 10 to 15% by weight of a yeast extract, 0.05 to 0.1% by weight of a turmeric extract, . ≪ / RTI > If it exceeds the above range, the increase in the efficacy is not high compared with the increase in the amount, so that there is no economical efficiency. If the above range is exceeded, the expected immune enhancing effect may not be exhibited.

As used herein, the term "vegetable lactic acid bacteria" refers to bacteria that produce lactic acid using a plant material (e.g., fruit) as a medium. As shown in the specific examples of the present invention, vegetable lactic acid bacteria are useful for enhancing immune function because they increase the production of INF-γ and IL-12 in vivo and are excellent in acid resistance (see Tables 1 to 3 of the Examples) ). Examples of the vegetable lactic acid bacteria that can be used in the present invention include Lactobacillus sp.), the genus Pediococcus sp.), Leuconostoc sp. sp.), the genus Weissella sp.) and Lactococcus genus (Lactococcus sp.), and the like, but it is not limited thereto as long as it corresponds to vegetable lactic acid bacteria. Preferably, Pediococcus ( Pediococcus) pentosaceus NB-17 may be used.

The term "yeast extract" as used herein refers to an extract derived from cells of yeast, which is widely used in foods such as baker's yeast, brewer's yeast, milk yeast and Tetora's yeast, and includes nucleic acids, amino acids, proteins, peptides, vitamins, And salts such as phosphoric acid. Preferably, the yeast extract of the present invention can be used with an RNA content of 10% or more, more preferably 30% or more.

The term " curcuma " langa extract "refers to an extract of turmeric, which is a perennial plant with ginger, and is not particularly limited to an extraction site, but is preferably extracted from a root or stem. The turmeric extract is obtained by a conventional method for producing an extract For example, the extract can be extracted using an extraction solvent such as an organic solvent or water, or can be obtained by a method such as a cold extraction method, a warm extraction method, a heat extraction method, or an ultrasonic extraction method. A fractionation solvent is added to the above extract Therefore, the turmeric extract of the present invention can be filtered, concentrated, or dried to remove the solvent. Thus, the dried turmeric extract of the present invention can be obtained by extracting a concentrated liquid, ≪ / RTI >

The inventors of the present invention found that when the composition of the present invention comprising water-soluble salmon extract and plant lactic acid bacteria was administered to experimental rats, the concentrations of interferon (INF-γ) and interleukin (IL-12) .

Specifically, in the examples of the present invention, 30 mice were administered with the compositions at different concentrations for 4 months after preparing a composition containing water-soluble salmon roe extract and vegetable lactic acid bacteria (Table 4 of the example). As a result, The concentration of INF-γ and IL-12 was only 10 ng / ml before administration of the composition of the present invention, but the concentration of INF-γ increased to 145-190 ng / ml after 4 months of administration and the concentration of IL- It was confirmed that it was significantly increased from 1000 ng / ml to 1350 mg / ml (Examples Table 5 and Table 6). These results far exceeded the results of the single administration of vegetable lactic acid bacteria (Examples 1 and 2).

INF-γ is known to activate the cytotoxic T lymphocyte and macrophage immune function, and IL-12 is known to activate macrophages and regulate the cellular immune response. Since the composition of the present invention has been confirmed to increase the concentrations of INF-γ and IL-12 in in vivo, it can be developed as a composition for improving immunity.

Furthermore, the composition of the present invention can be developed as a functional food or a pharmaceutical composition for the prevention, improvement or treatment of various immune diseases requiring immunity enhancement, for example, cancer or allergy.

In relation to cancer, it is known that INF-γ acts to inhibit the proliferation of cancer cells, and IL-12 is known as a substance that assists activation of killer cells attacking cancer. The composition of the present invention, And can be effective in preventing or treating cancer.

In addition, the allergic reaction is known to be caused by an imbalance of Th1 helper cell type 1 and Th helper cell type 2. INF-γ and IL-12 are cytokines of Th1, The composition of the present invention can effectively work to prevent or treat allergy.

When the composition of the present invention is used as a pharmaceutical composition, it may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions, and may be formulated into powders, granules, tablets, capsules, suspensions, Emulsions, syrups, aerosols and the like, external preparations, suppositories or sterilized injection solutions, and the like. More specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like, and such solid preparations may contain at least one excipient and / or a lubricant. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration may include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, suppositories, and the like.

The preferred dosage of the composition varies depending on the condition and the weight of the patient, the degree of the disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. For a more preferable effect, the dose of the composition of the present invention is preferably 3,000 mg per day based on an adult of 60 kg, but is not limited thereto. The administration may be carried out once a day or divided into several doses. The compositions of the present invention may be administered to a mammal, including a human, in various ways. All modes of administration may be expected, for example, by oral, intravenous, intramuscular, subcutaneous injection, and the like. The pharmaceutical dosage form of the composition of the present invention may be used in the form of a pharmaceutically acceptable salt of the active ingredient, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable combination.

When the composition of the present invention is used as a food composition, it may contain an acceptable food-aid additive and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.

In the present invention, the term " food " means a natural product or a processed product containing one or more nutrients, preferably a state of being able to be directly eaten through a certain processing step, , Health functional foods, beverages, food additives and beverage additives. Examples of the food include various foods, beverages, gums, tea, vitamin complex, and functional foods. In addition, in the present invention, the food may contain special nutritional foods (e.g., crude oil, spirits, infant food, etc.), meat products, fish products, tofu, jelly, noodles (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, natural flavors (eg, ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages and other health supplement foods. The health functional food, beverage, food additive or beverage additive can be produced by a usual production method.

In the food, the amount of the composition of the present invention may be 0.00001% to 50% by weight of the total food. When the food is a beverage, the amount of the composition is 0.001 g to 50 g , Preferably from 0.01 g to 10 g, but is not limited thereto.

The composition comprising the water-soluble salmon roe extract of the present invention and the plant lactic acid bacteria induces the production of interferon (INF-γ) and interleukin (IL-12) which are immunological related physiologically active substances and activates the immune function. And further food and pharmaceutical applications are possible to prevent, ameliorate and treat cancer and allergy through immune function enhancement.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example  1. Assessment of Immune Function Activation Ability of Plant Lactic Acid Bacteria

Lactobacillus plantum SK1 ( Lactobacillus < RTI ID = 0.0 > plantum SK1 ), Lactobacillus plantum SK2 ( Lactobacillus plantum SK2 ), Lactobacillus plantum SK3 ( Lactobacillus plantum SK3 ), Lactobacillus sheep ( Lactobacillus sake), Casey Lactobacillus (Lactobacillus casei ), and Pediococcus (INF-γ) and interleukin (IL-12), respectively, were orally administered to experimental rats.

10 rats were orally administered with 1 mg of dried vegetable lactic acid bacterium per kg of the weight of the rat per day for 6 months, and the blood of the rats administered orally with the botanical lactobacillus was collected every month to obtain the immune-related physiologically active interferon (INF- γ) and interleukin (IL-12) were examined. Table 1 shows the results of the production of interferon (INF-γ) according to the administration of vegetable lactic acid bacteria. Table 2 shows the results of production of interleukin (IL-12) according to administration of vegetable lactic acid bacteria (unit: ng / ml). As shown in Table 1 and Table 2, all six vegetable lactic acid bacteria increased the production of INF-γ and IL-12, and in particular, Lactobacillus plantum SK3 ) and Pediococcus pentosaceus powder, the production of INF-γ and IL-12 was high.

(unit:
ng / ml) Control L. plantum SK1 L. plantum SK2 L. plantum SK3 L. sake L. casei P. pentosaceus 0 months 10 10 10 10 10 10 10 1 month 12 50 30 60 40 45 50 2 months 8 60 55 85 55 50 70 3 streams 14 100 65 120 70 90 99 4 months 14 125 95 150 80 110 135 5 months 10 120 100 160 75 105 120 6 months 10 135 95 160 85 105 130

(unit:
ng / ml) Control L. plantum SK1 L. plantum SK2 L. plantum SK3 L. sake L. casei P. pentosaceus 0 months 120 10 10 10 10 10 10 1 month 110 280 550 150 120 350 700 2 months 150 350 700 220 180 550 950 3 streams 160 580 750 350 380 680 1,200 4 months 110 610 700 480 400 750 1,250 5 months 120 550 720 550 360 740 1,200 6 months 130 600 750 550 400 700 1,150

Example  2. In the artificial gastric juice of the selected lactic acid bacteria Acid resistance  evaluation

Pediococcus ( Pediococcus) , which showed excellent ability to induce the production of an immunological-related bioactive substance in Example 1 above, pentosaceus ) and Lactobacillus plantum SK3 ( Lactobacillus plantum SK3 ) were tested for acid resistance by using artificial gastric juice of pH 2.5.

As shown in Table 3 below, the survival rate of Pediococcus pentosaceus was 90% or more in artificial gastric juice of pH 2.5 after 3 hours in the case of Pediococcus pentosaceus as a result of confirming acid resistance using artificial gastric juice. This shows that the selected Lactobacillus can be safely reached to the intestines without dying on oral administration. On the other hand, Lactobacillus Term Plan SK3 (Lactobacillus plantum SK3 ) survived about 10% at 3 hours and were found to be very weak in stomach acid compared with Pediococcus pentosaceus .

Processing time
Strain name 0 hours 1 hours 2 hours 3 hours 4 hours 5 hours Pediococcus pentosaceus 1 * 10 ¹⁰ 1 * 10 ¹⁰ 1 * 10 ¹⁰ 0.9 * 10 ¹⁰ 0.85 * 10 ¹⁰ 0.8 * 10 ¹⁰ Lactobacillus
plantum SK3 1 * 10 ¹⁰ 0.9 * 10 ¹⁰ 0.5 * 10 ¹⁰ 1 * 10 ⁹ 0.6 * 10 ⁹ 0.2 * 10 ⁹

Example  3. Assessment of Immune Function Activation Ability of Salmon Root Extract and Plant Lactic Acid Bacteria

A composition according to the compositional table of Table 4 below was prepared as a composition containing water-soluble salmon roe extract and vegetable lactic acid bacteria.

ingredient Content (mg) ratio Water-soluble salmon ginseng extract
(SALMON MILT EXTRACT) 375.0 37.5% Yeast extract
(YEAST EXTRACT) 125.0 12.5% Pearl keratin calcium
(PERL OYSTER CALCIUM) 40.0 4.0% Vitamin C 22.0 2.2% Vegetable lactic acid bacteria NB-17
(Pediococcus pentosaceus) 4.5 0.45% Nicacinic acid (NIACIN) 4.0 0.4% Vitamin A 0.8 0.08% Vitamin B1
(Thiamine Hydrochloride) 0.4 0.04% Vitamin B2 0.4 0.04% Vitamin B6
(Pyridoxine Hydrochloride) 0.6 0.06% Vitamin D3 0.07 0.007% Vitamin B12 0.53 0.053% Magnesium oxide
(MAGNESIUM OXIDE) 20.0 2.0% Collagen (pork skin)
(COLLAGEN (PORK SKIN)) 16.0 1.6% GLUCOSAMINE 5.0 0.5% SHARK CARTILAGE 3.0 0.3% Hyaluronic acid (HYALURONIC ACID) 3.0 0.3% Turmeric extract (TURMERIC EXTRACT POWDER) 0.7 0.07% Excipients, fragrances, sweeteners 382.0 38.2% Sum 1,000.0 100.0%

For 40 rats, immuno-related efficacy studies were performed using the composition according to Table 4 above. 30 rats were orally administered with 25 mg, 50 mg and 100 mg of the composition per kg of the mice once a day for 4 months, and 10 rats were used as a control. Blood was collected from rats fed orally with the above composition every month to examine changes in the concentration of interferon (INF-γ) and interleukin (IL-12), which are immunological related physiologically active substances. The method for measuring the concentrations of INF-y and IL-12 is the same as in Example 1.

Table 5 shows the results of the production of interferon (INF-y) upon administration of the composition of the present invention, and Table 6 shows the results of the production of interleukin (IL-12). As shown in Tables 5 and 6, when administered at a dose of 50 mg or more per kg of rat weight, the levels of interferon (INF-γ) and interleukin (IL-12) as immuno-related physiologically active substances were significantly higher than those before administration. Using these results, a daily dose of about 3,000 mg can be determined on a 60 kg adult basis.

(Unit: ng / ml) Control 25mg / Kg 50mg / Kg 100 mg / Kg 0 months 10 10 10 10 1 month 12 40 70 80 2 months 8 70 100 110 3 streams 14 110 180 180 4 months 14 145 190 185

(Unit: ng / ml) Control 25mg / Kg 50mg / Kg 100 mg / Kg 0 months 120 10 10 10 1 month 110 700 850 900 2 months 150 850 1,050 1,100 3 streams 160 900 1,200 1,300 4 months 110 1,000 1,300 1,350

Claims (9)

A water-soluble salmon ginseng extract, and a vegetable lactic acid bacterium. The food composition according to claim 1, wherein the vegetable lactic acid bacterium is Pediococcus pentosaceus . The food composition of claim 1, further comprising yeast extract, turmeric extract or a combination thereof. The method according to claim 1, wherein the yeast extract, pearl keratinocyte, vitamin C, nicotinic acid, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin D3, vitamin B12, magnesium oxide, collagen, glucosamine, shark cartilage, hyaluronic acid, An extract, an excipient, a flavoring, and a sweetener. The composition according to claim 4, which comprises 30 to 40% by weight of a water-soluble salmon extract, 0.25 to 0.70% by weight of vegetable lactic acid bacteria, 10 to 15% by weight of yeast extract, 0.05 to 0.1% And a sweetener. The food composition according to claim 1, which has a function of preventing or improving cancer through immunization. The food composition according to claim 1, which has a function of preventing or improving allergy through immune enhancement. A water-soluble salmon extract, and a vegetable lactic acid bacterium. A pharmaceutical composition for the prevention or treatment of allergy, comprising a water-soluble salmon extract and a vegetable lactic acid bacterium.