KR20210024357A - Antioxidant and moisturizing cosmetic composition containing mixed extracts including Prunus yedoensis flower, Peach tree flower and Chrysanthemum flower extract - Google Patents

Abstract

The present invention relates to an antioxidant and skin moisturizing cosmetic composition containing a mixed extract including Prunus × yedoensis flower, Prunus persica flower and Chrysanthemum flower extracts. According to the present invention, it is possible to provide the antioxidant and skin moisturizing cosmetic composition containing a mixed extract including Prunus × yedoensis flower, Prunus persica flower and Chrysanthemum flower extracts.

Description

Antioxidant and moisturizing cosmetic composition containing mixed extracts including Prunus yedoensis flower, Peach tree flower and Chrysanthemum flower extract}

The present invention relates to an antioxidant and moisturizing cosmetic composition containing a mixed extract comprising a Yoshino cherry tree flower, a radish tree flower and a chrysanthemum flower extract, and more specifically, a Yoshino cherry tree flower, a radish tree flower, a chrysanthemum flower, and other extracts. It relates to an antioxidant and moisturizing cosmetic composition containing a mixed extract comprising a Yoshino cherry tree flower, a radish tree flower and a chrysanthemum flower extract having more effective antioxidant and moisturizing functions by containing the mixed extract containing it.

As human skin ages, various internal and external factors change it.

In other words, internally, the secretion of various hormones that regulate metabolism decreases, the function of immune cells and the activity of cells decrease, thereby reducing the biosynthesis of immune proteins and biological components necessary for the living body, and externally, due to the destruction of the ozone layer. As a result, the amount of ultraviolet rays reaching the surface of the sun's rays increases, and as environmental pollution becomes more intense, free radicals and active harmful oxygen increase, so that the thickness of the skin decreases, wrinkles increase, and elasticity decreases. In addition, the complexion of the skin becomes dull, and skin problems occur frequently, and various changes occur, such as increased spots, freckles and age spots.

As aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, and glycoproteins, which are substances constituting the skin, change or decrease. Symptoms arise, and oxidative stress due to free radicals and free radicals and free radicals occurs. In addition, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokines, is known to cause inflammation in most cells that make up the skin due to aging or ultraviolet rays. Biosynthesis increases, and the biosynthesis of matrix metalloproteinase (MMP), an enzyme that degrades skin tissue by these inflammatory factors, increases, and NO (nitric oxide) production by iNOS (inducible nitric oxide synthase) is increased. It is known to increase.

In other words, the biosynthesis of substrate materials is reduced due to the decrease in cellular activity and micro-inflammation due to the naturally occurring endogenous aging, and external factors such as an increase in stress due to various harmful environments and an increase in reactive oxygen species caused by sunlight. As a result, decomposition and degeneration are accelerated, the skin substrate is destroyed and thinner, leading to various symptoms of skin aging.

Therefore, it is a reality that many studies are being conducted on active ingredients that can prevent and improve such aging phenomena. As an example, Korean Patent Publication No. 10-2002-0080657 has been disclosed.

On the other hand, the skin is a representative organ that exhibits an immune response while retaining moisture in the body and performing an essential barrier function to protect the inside from the invasion of external factors.

Here, hyaluronic acid (HA), a representative factor that maintains skin moisture, is one of the three major elements of the skin along with collagen and elastin, and has a strong hydrophilic property to form a gel by combining with water, which plays an important role in maintaining skin moisture. It is known as a natural moisturizer.

Therefore, substances that induce the synthesis of hyaluronic acid or inhibit the decomposition are effective in moisturizing the skin and preventing aging.In particular, among hyaluronic acid synthase (HAS), HAS2 and HAS3 are known to play a crucial role in the synthesis of hyaluronic acid. , Recently, studies to promote the production of hyaluronic acid through the increase of HAS gene expression have been actively conducted.

Accordingly, in the present invention, it was confirmed that extracts such as Yoshino cherry tree flowers, radiant tree flowers, and chrysanthemum flowers among the Namwon-Jiri mountain area resource plants have antioxidant and moisturizing functions, and accordingly, Yoshino cherry tree flowers, radiant tree flowers, chrysanthemum flowers, and others. It is intended to develop a cosmetic composition with excellent antioxidant and moisturizing functions using extracts.

In order to solve the above problems, the present invention contains a Yoshino cherry tree flower, a radish tree flower, a chrysanthemum flower, and a mixed extract including other extracts, so that the antioxidant and moisturizing functions are more effective, such as Yoshino cherry tree flowers, radish tree flowers, and chrysanthemums. It is an object to provide an antioxidant and moisturizing cosmetic composition containing a mixed extract containing a flower extract.

In order to solve the above problems, the antioxidant and moisturizing cosmetic composition according to an embodiment of the present invention provides an antioxidant and moisturizing cosmetic composition containing a mixed extract including a Yoshino cherry tree flower, a radish tree flower and a chrysanthemum flower extract. can do.

Here, the Yoshino cherry tree flower extract, the radish tree flower extract, and the chrysanthemum flower extract are characterized in that the Yoshino cherry tree flower, the radish tree flower, and the chrysanthemum flower are extracted using one of water or an organic solvent as a solvent, respectively.

In addition, the mixed extract is characterized in that it comprises a Yoshino cherry tree flower extract, a radix tree flower extract, and a chrysanthemum flower extract in a weight ratio of 1.5 to 3.5: 1.5 to 2.5: 2 to 3.

In addition, the antioxidant and moisturizing cosmetic composition is characterized in that it contains 5 to 10% by weight of the mixed extract based on the total weight of the cosmetic composition.

In addition, the mixed extract may further include sapodilla fruit extract and peppermint fermentation broth.

These antioxidant and moisturizing cosmetic compositions include emulsions, creams, lotions, essences, packs, suspensions, pastes, gels, powders, lipsticks, makeup bases, foundations, lotions, ointments, gels, patches, essences, cleansing foams, and cleansing creams. , Cleansing water, soap, shampoo, rinse, and spray formulation.

Antioxidant and moisturizing cosmetic composition containing a mixed extract comprising a Yoshino cherry tree flower, a radish tree flower, and a chrysanthemum flower extract according to an embodiment of the present invention as described above is a Yoshino cherry tree flower, a radish tree flower, a chrysanthemum flower and other extracts By containing the mixed extract containing, it has an antioxidant and moisturizing function, and by using together, it is complementary to each other, and the antioxidant and moisturizing function may be more effective than when each extract is used alone.

Hereinafter, the description of the present invention with reference to the drawings is not limited to a specific embodiment, and various transformations may be applied and various embodiments may be provided. In addition, the content described below should be understood to include all conversions, equivalents, and substitutes included in the spirit and scope of the present invention.

In the following description, terms such as first and second are terms used to describe various elements, and their meanings are not limited, and are used only for the purpose of distinguishing one element from other elements.

The same reference numbers used throughout this specification denote the same elements.

The singular expression used in the present invention includes a plurality of expressions unless the context clearly indicates otherwise. In addition, terms such as "comprise", "include" or "have" described below are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification. It is to be construed and not to preclude the possibility of the presence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

An antioxidant and moisturizing cosmetic composition (hereinafter referred to as a'cosmetic composition') containing a mixed extract comprising a Yoshino cherry tree flower, a radish tree flower and a chrysanthemum flower extract according to an embodiment of the present invention is a Yoshino cherry tree flower, a radiant tree flower, and By containing the mixed extract containing the chrysanthemum flower extract, it is possible to provide a cosmetic composition superior in antioxidant and moisturizing functions.

First, the Yoshino cherry tree extract contained in the mixed extract has the ability to produce hyaluronic acid and contains polyphenols and flavonoids, so it can effectively reduce free radicals through antioxidant effects and protect cells, thereby preventing skin aging.

In addition, the radix tree flower extract can express HAS2 and HAS3 related to skin moisturizing, and it contains polyphenols and flavonoids, so it can exhibit antioxidant effects, supply nutrition to the skin, and to create vitality in the skin as a conditioning role. Can help.

In addition, chrysanthemum flower extract has excellent hyaluronic acid production ability, has excellent moisturizing effect, can hold dryness and remove dead skin cells that cause moisture loss.It contains polyphenols and flavonoids, so it has an antioxidant effect. , Vitamin A, B1, C and other nutrients are rich, so it can help improve skin diseases such as atopy and hives.

As described above, Yoshino cherry tree extract, radix tree flower extract, and chrysanthemum flower extract have the same antioxidant and moisturizing functions, but there is a difference in efficacy in detail, such as Yoshino cherry tree extract, radix tree flower extract, and chrysanthemum flower extract are mixed. By using it as a mixed extract, the present invention allows the antioxidant and moisturizing functions to appear more effectively.

These Yoshino cherry tree flower extracts, radiant tree flower extracts, and chrysanthemum flower extracts can be prepared by extracting Yoshino cherry tree flowers, radiant tree flowers, and chrysanthemum flowers using one of water or an organic solvent as a solvent, respectively.

The Yoshino cherry tree flowers, radiant tree flowers, and chrysanthemum flowers used here are resource plants in the Namwon and Jirisan mountains, and can be harvested and dried in Namwon City, and dried for 24 hours to 72 hours at 35 to 45°C, and at 42°C. It is preferable to use one dried for 48 hours.

At this time, if the drying temperature is less than 35°C, the drying time may take too long or water evaporation is not well performed, so that the extraction efficiency may decrease. If the drying temperature exceeds 45°C, burning, destruction or deformation of the active ingredient may occur. .

In addition, when the drying time is less than 24 hours, drying is not performed well, and when the drying time exceeds 72 hours, since drying is sufficiently performed, it is inefficient, and the extraction efficiency may be lowered.

The organic solvent is preferably ethanol or 1,3-butylene glycol, but methanol, isopropanol, butanol, and the like may also be used. In addition, when using ethanol, it is preferable to use 50% ethanol, but this is only an example of the present invention, so it is not limited thereto.

More specifically, the Yoshino cherry tree flower extract or the radiant tree flower extract or the chrysanthemum flower extract is a raw material (Yoshino cherry tree flower or radish tree flower or chrysanthemum flower) 4 to 6 parts by weight of 95 to 100 parts by weight of water or an organic solvent added and heated After that, it can be concentrated and lyophilized to prepare. At this time, it is preferable to add 95 parts by weight of water or an organic solvent to 5 parts by weight of the raw material.

In this case, if the raw material is less than 4 parts by weight, the efficacy of the extract appears less, and if it exceeds 6 parts by weight, it may affect the scent and color, which are the organoleptic properties of the cosmetic.

In addition, if the solvent is less than 95 parts by weight, the active ingredient is not completely leached and the extract is concentrated even if only slightly evaporated, and the active ingredient in the extract may be locally destroyed by excessive heat. If the amount exceeds 100 parts by weight, the amount of the active ingredient is increased. It can be done, but it is inefficient when lyophilized.

On the other hand, when water is used as a solvent, it can be heated at 95 to 105°C for 230 to 250 minutes, and when an organic solvent is used, it can be heated at 60 to 85°C for 3 to 12 hours, preferably when using water It can be heated at 100° C. for 240 minutes or at 80° C. for 4 hours when using an organic solvent.

At this time, when using water, the temperature is 95°C, the time is 230 minutes, when using an organic solvent 60°C, if less than 3 hours, the active ingredient may not be completely extracted. When using water, the temperature is 105°C and the time is 250 When using an organic solvent, if it exceeds 85°C for 12 hours, the active ingredient may be destroyed, and the yield of the active ingredient to be extracted no longer increases, so further extraction is meaningless.

A mixed extract can be prepared by combining the extracted Yoshino cherry tree flower extract, radish tree flower extract, and chrysanthemum flower extract. Its antioxidant and moisturizing functions can be improved.

That is, the mixed extract made by mixing the Yoshino cherry tree flower extract, the radix tree flower extract, and the chrysanthemum flower extract may contain each extract in a weight ratio of 1.5 to 3.5: 1.5 to 2.5: 2 to 3, and 3.5: 1.5: 2 or It is more preferable to include it in a weight ratio of 1.5:2.5:3.

When included in such a weight ratio, it can exhibit the most desirable antioxidant and moisturizing functions.

In addition, the mixed extract may contain 5 to 10% by weight based on the total weight of the cosmetic composition, and 7% by weight is most preferred.

At this time, with respect to the total weight of the cosmetic composition, if the mixed extract is less than 5% by weight, the antioxidant and moisturizing function is insignificant, and if it exceeds 10% by weight, allergic reactions, itching, itching, etc. may occur, so there is a problem in skin safety. And can affect the organoleptic properties.

In addition, the mixed extract of the cosmetic composition according to an embodiment of the present invention may further include sapodilla fruit extract and peppermint fermentation liquid to improve antioxidant effect and preservation.

Sapodilla fruit extract removes free radicals to prevent aging, contains vitamin A to help maintain healthy mucous membranes and skin, and contains tannin, a polyphenol, to help with dermatitis with anti-inflammatory and soothing effects. By giving, the dried sapodilla fruit can be prepared by extracting the dried sapodilla fruit using either water or an organic solvent as a solvent, and the extraction conditions can be extracted under the same conditions as the other extracts.

Peppermint fermentation broth has antioxidant and antibacterial effects, reduces evaporation of moisture, and improves the moisturizing effect, and improves the preservability of cosmetic compositions. A mixture of peppermint extract with xylitol powder, moringa powder, and cornelian milk powder. After fermentation by adding lactic acid bacteria can be prepared.

The peppermint extract used at this time is effective in alleviating allergic symptoms because it contains peppermint antioxidants and has an anti-inflammatory effect. It can be prepared by extracting dried peppermint using either water or an organic solvent as a solvent. Conditions can be extracted under the same conditions as the other extracts.

In addition, xylitol powder can reduce evaporation of moisture so that a moisturizing effect can be exhibited excellently, and it can be helpful for skin health.

In addition, moringa powder and cornus powder can improve the preservability of cosmetic compositions through antibacterial and purifying effects.

The lactic acid bacteria are preferably Bifidobacterium bifidum and Streptococcus thermophilus, but are not limited thereto.

Specifically, mint fermentation broth is prepared by mixing 20 to 30 parts by weight of xylitol powder, 0.1 to 0.5 parts by weight of moringa powder, 0.1 to 1 part by weight of cornus powder, and 0.1 to 1 part by weight of cornflower powder to 100 parts by weight of peppermint extract, and then adding lactic acid bacteria and naturally fermenting at room temperature. Can be. After that, it can be filtered and used.

At this time, if the amount of xylitol powder is less than 20 parts by weight, the moisturizing function improvement effect may be insignificant, and if it exceeds 30 parts by weight, the sensory properties of the cosmetic composition may be affected.

If the moringa powder is less than 0.1 parts by weight, the efficacy of the moringa powder is insignificant, and if it exceeds 0.5 parts by weight, there may be a problem in skin safety.

If the amount of cornus oil powder is less than 0.1 part by weight, the efficacy of the cornus oil powder is insignificant, and when it exceeds 1 part by weight, skin safety problems may occur and the sensory properties of the cosmetic composition may be affected.

The thus prepared peppermint fermentation broth is prepared by fermentation with lactic acid bacteria, so that the efficacy of each material is more excellent, so that it can effectively act on improving the function of the cosmetic composition.

These sapodilla fruit extract and peppermint fermentation broth may be further included in 2 to 2.5 parts by weight and 0.5 to 1 parts by weight, respectively, in the mixed extract.

In this case, when the sapodilla fruit extract and the peppermint fermentation broth are further included in each of the above ranges, synergistic effects such as antioxidant and moisturizing function appearing as they are further included are insignificant, and when the sapodilla fruit extract and the peppermint fermentation broth are respectively less than the above range, a problem may occur in skin safety.

That is, the mixed extract includes a Yoshino cherry tree flower extract, a radish tree flower extract, a chrysanthemum flower extract, a sapodilla fruit extract, and a mint fermentation broth in a weight ratio of 1.5 to 3.5: 1.5 to 2.5: 2 to 3: 2 to 2.5: 0.5 to 1 It can be, and it is preferable to include in a weight ratio of 3.5: 1.5: 2: 2.5: 0.5 or 1.5: 2.5: 3: 2.5: 0.5.

Antioxidant and moisturizing cosmetic compositions containing such mixed extracts include emulsions, creams, lotions, essences, packs, suspensions, pastes, gels, powders, lipsticks, makeup bases, foundations, lotions, ointments, gels, patches, essences, It may be formed as a cleansing foam, cleansing cream, cleansing water, soap, shampoo, conditioner, spray formulation, but is not limited thereto.

As described above, the cosmetic composition for antioxidant and moisturizing containing a mixed extract comprising a Yoshino cherry tree flower, a radish tree flower and a chrysanthemum flower extract according to an embodiment of the present invention is a Yoshino cherry tree flower, a radish tree flower, a chrysanthemum flower and By containing a mixed extract including other extracts, it has antioxidant and moisturizing functions, and by using them together, it is complementary to each other, so that the antioxidant and moisturizing functions may be more effective than when each extract is used alone.

Hereinafter, the experimental examples to be carried out are provided to aid in understanding, and are merely illustrative of the present invention, and the scope of the present invention is not limited to the following experimental examples.

In addition, in order to find out the effect of the cosmetic composition, examples and experimental examples were described using water and ethanol as solvents, but when preparing the cosmetic composition, 1,3-butylene glycol ) Can be used mainly.

[Preparation Example 1] Preparation of Yoshino cherry tree flower extract

The Namwon-Jiri mountain region resource plants, Yoshino cherry tree flowers, were dried, 95 parts by weight of 50% ethanol was added to 5 parts by weight of the dried Yoshino cherry tree flowers, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 2] Preparation of radix tree flower extract

A radiant tree flower, a resource plant in the Namwon-Jiri mountain area, was dried, 95 parts by weight of 50% ethanol was added to 5 parts by weight of the dried radiant tree flower, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 3] Preparation of chrysanthemum flower extract

Chrysanthemum flowers, a resource plant in the Namwon-Jiri Mountain region, were dried, 95 parts by weight of 50% ethanol was added to 5 parts by weight of the dried chrysanthemum flowers, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 4] Preparation of sapodilla fruit extract

95 parts by weight of 50% ethanol was added to 5 parts by weight of dried sapodilla fruit, heated at 80° C. for 4 hours, concentrated, and freeze-dried to prepare.

[Preparation Example 5] Preparation of peppermint fermentation broth

100 parts by weight of peppermint extract was prepared by mixing 20 parts by weight of xylitol powder, 0.5 parts by weight of moringa powder, and 1 part by weight of cornus powder, followed by addition of lactic acid bacteria and natural fermentation at room temperature.

[Examples 1 and 2] Preparation of mixed extract

Preparation Examples 1 to 5 were mixed according to the weight ratio of Table 1 to prepare the mixed extracts of Examples 1 and 2.

[ Experimental example 1] Contents of polyphenols and flavonoids

In order to confirm whether it has an antioxidant function, the contents of polyphenols and flavonoids of Preparation Examples 1 to 3 were measured by the following method.

For polyphenols, 80 µl of Preparation Examples 1 to 3 and 20 µl of 1N Folin reagent were added to a 96-well plate, incubated for 5 minutes at room temperature, _{100 µl of 2% Na 2} CO ₃ reagent was added, and then at room temperature for 30 minutes. After incubation, ELISA absorbance was measured at 750 nm.

For flavonoids, 10 μl of Preparation Examples 1 to 3 and 100 μl of diethylene glycol reagent were added to a 96-well plate, 10 μl of 1N NaOH was added, and then incubated at 37° C. for 1 hour, and the ELISA absorbance was measured at 420 nm.

The results are shown in Table 2 below.

As can be seen from Table 2, it was confirmed that all of Preparation Examples 1 to 3 contained polyphenols and flavonoids.

Accordingly, it was determined that all of the Yoshino cherry tree flower extract, the radix tree flower extract, and the chrysanthemum flower extract of Preparation Examples 1 to 3 can exhibit an antioxidant function.

[ Experimental example 2] Antioxidant function

In order to confirm the antioxidant function, the extracts of Preparation Examples 1 to 3 and Examples 1 and 2 were measured for free radical scavenging activity (SC) in the following manner to evaluate the antioxidant function.

DPPH (1,1-diphenyl-2-picrylhydrazyl radical) solution (60 M) was prepared. Then, the concentration of Examples 1 to 3 was adjusted to 1%, and the DPPH solution was mixed in a 1:1 ratio, followed by vortexing (vortex mixing). After reacting at room temperature for 30 minutes, absorbance was measured at 520 nm to obtain free radical scavenging activity (SC) according to the formula of Equation 1 below.

Ascorbic acid was used as a positive control.

[Equation 1]

Free radical scavenging ability (%) = (control absorbance-Example absorbance) / (control absorbance) X100

The results are shown in Table 3 below.

As can be seen from Table 3 above, it was confirmed that Preparation Examples 1 to 3, Examples 1 and 2 all showed higher free radical scavenging ability than the control group.

In particular, it could be confirmed that the free radical scavenging ability of Examples 1 and 2 was particularly high.

This is because Preparation Examples 1 to 3 may exhibit an antioxidant function, but it is judged that the antioxidant function is higher when used in combination than when used alone.

[ Experimental example 3] Moisturizing function

In order to confirm the moisturizing effect, the extracts of Preparation Examples 1 to 3 and Examples 1 and 2 were analyzed for their moisturizing function by measuring hyaluronic acid secretion ability and the expression level of HAS2 and HAS3 in the following manner.

Hyaluronic acid secretion ability was determined by using DMEM (High glucose, Welgene, Daegu, Korea) containing heat inactivation 10% fetal bovine serum (Merck, MA, USA) and 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA). 5% CO ₂ , cultured in a cell incubator at 37°C. Each sample was treated at a concentration of 50 μg/mL for 48 hours, and then measured by HA ELISA.

The expression level of HAS2 and HAS3 was determined ^{by dispensing HaCaT cells into 6x10 5} cells in a 6 well plate and incubating for 24 hours, washing twice with FBS free medium, replacing with FBS free medium, and treating the sample with 50 μg/ml. Incubated for 48 hours. To extract RNA from cells after 48 hours, tri reagent (RNAiso PLUS, Takara, Otsu, Japan), which is a total RNA extraction reagent, was used. 1 mL of Tri reagent was added to the cells and vortexed for 15 seconds. Then, after centrifugation at 15,000 rpm for 15 minutes, 200 µl of chloroform was added, vortexed for 20 seconds, centrifuged, and then the supernatant was transferred to a new tube. An equal amount of cold isopropanol was added and left at room temperature for 10 minutes. This was again centrifuged at 15,000 rpm for 20 minutes to obtain an RNA precipitate. After washing the RNA precipitate with 75% ethanol, the precipitate was dried at room temperature and dissolved in 0.1% diethyl pyrocarbonate (DEPC) water. RNA concentration (1 OD=40 μg/mL) was measured at 260 nm using an Evloution 260 Bio UV-Vis spectrophotometer (Thermo Scientific, CA, USA), and then reverse transcription kit (RNA to cDNA EcoDry Premix (Oligo dT), Clontech, USA) was used to synthesize cDNA from 1 μg RNA. After the synthesized cDNA was diluted 7 times with sterile distilled water, real-time PCR was amplified in Light Cycler 2.0 (Roche) using Fast Start DNA MasterPLUS SYBR Green I kit (Roche, Mannheim, Germany).

PCR conditions were carried out at 50° C. for 60 minutes.

ATRA was used as a brewing control group.

The results are shown in Table 4 below.

As can be seen from Table 4, it was confirmed that the hyaluronic acid secretion ability appeared in Preparation Examples 1 and 3, and Examples 1 and 2, and it was confirmed that the hyaluronic acid secretion ability appeared higher than that in Examples 1 and 2.

In addition, it was confirmed that HAS2, HAS3 expression occurs only in Preparation Example 2, Examples 1 and 2.

This is because Preparation Examples 1 to 3 may exhibit a moisturizing effect, but it is judged that the moisturizing effect will be higher when used in combination than when used alone.

[ Formulation example 1 to 5] Essence Preparation

Formulation Examples 1 to 5, which are cosmetic compositions, were prepared using the extracts of Preparation Examples 1 to 3 and Examples 1 and 2, respectively, and are shown in Table 5 below.

[ Experimental example 4] Check the skin moisturizing effect

In order to confirm the moisturizing effect of Formulation Examples 1 to 5, 50 healthy women aged 30 to 50 were tested.

In order to confirm the effect of improving skin moisturization, Formulation Examples 1 to 4 were divided into 10 people and applied twice a day (morning and evening) to the face for 2 weeks.

Before the experiment begins, determine the 2 areas to be measured, measure the moisture content of the skin 3 times, apply for 2 weeks (2 times/day), and then use the skin moisture content measuring device to use the epidermal layer just below the stratum corneum. The moisture of the was measured. The measurement results are shown in Table 6.

As can be seen from Table 6, it can be seen that Formulation Examples 1 to 3 exhibit a moisturizing effect, but it was confirmed that formulation Examples 4 and 5 exhibit a higher moisturizing effect.

[ Experimental example 5] Preservation evaluation

Formulation Examples 4 and 5 were stored at a constant temperature and humidity (high temperature, 40°C, and the pH change was measured periodically for 10 weeks to investigate the preservability of each formulation example, and the results are shown in Table 7).

As can be seen in Table 7, it was confirmed that the pH change of Formulation Example 5 was less than that of Formulation Example 4.

Accordingly, it is judged that Formulation Example 5 has better storage properties than Formulation Example 4.

The embodiments of the present invention have been described above with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains can be implemented in other specific forms without changing the technical spirit or essential features of the present invention. You will be able to understand. Therefore, the embodiments described above are illustrative and non-limiting in all respects.

Claims (6)

Antioxidant and moisturizing cosmetic composition containing a mixed extract comprising a Yoshino cherry tree flower extract, a radish tree flower extract and a chrysanthemum flower extract.
The method of claim 1,
The Yoshino cherry tree flower extract, radix tree flower extract, and chrysanthemum flower extract,
Antioxidant and moisturizing cosmetic composition, characterized in that the Yoshino cherry tree flowers, radiant tree flowers, and chrysanthemum flowers are each extracted using one of water or an organic solvent as a solvent.
The method of claim 1,
The mixed extract,
An antioxidant and moisturizing cosmetic composition comprising a Yoshino cherry tree flower extract, a radix tree flower extract, and a chrysanthemum flower extract in a weight ratio of 1.5 to 3.5: 1.5 to 2.5: 2 to 3.
The method of claim 1,
The antioxidant and moisturizing cosmetic composition,
Antioxidant and moisturizing cosmetic composition comprising 5 to 10% by weight of the mixed extract based on the total weight of the cosmetic composition.
The method of claim 1,
The mixed extract,
An antioxidant and moisturizing cosmetic composition further comprising sapodilla fruit extract and peppermint fermentation broth.
The method of claim 1,
The antioxidant and moisturizing cosmetic composition,
Emulsion, cream, lotion, essence, pack, suspension, paste, gel, powder, lipstick, makeup base, foundation, lotion, ointment, gel, patch, essence, cleansing foam, cleansing cream, cleansing water, soap, shampoo, rinse , Antioxidant and moisturizing cosmetic composition, characterized in that the formulation of a spray.