KR20180007957A - Method for red sea cucumber aquaculture using adhesive microalgae - Google Patents

Abstract

The present invention relates to a method of farming red sea cucumbers by using adhesive microalgae. The method includes: a step (1) of attaching Achnanthes brevipes var. intermedia, Achnanthes sancti pauli, and Melosira octogona by forming an adhesive substrate horizontally on the floor of a water tank having a predetermined volume and collecting and communicating seawater of Jeju island; a step (2) of cultivating the adhesive bacillariophyceae through sunlight by moving the adhesive substrate with the adhesive bacillariophyceae to an outdoor water tank; a step (3) of collecting and fixing the substrate vertically to a red sea cucumber farming tank; and a step (4) of simultaneously cultivating red sea cucumbers and the adhesive microalgae in the red sea cucumber farming tank. As such, the present invention is capable of farming sea cucumbers safely and economically by using adhesive microalgae collected from seawater and cultivated.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for forming red sea germs using an adhesive microalga,

The present invention relates to a method for culturing sea cucumbers using naturally occurring adherent microalgae isolated from Jeju lava seawater and using adherent microalgae which are used as food in the red sea gypsy culture.

About 1500 species of sea cucumber are known all over the world. There are about 14 kinds of sea cucumber which belong to the pure sea cucumber sea cucumber and the sea cucumber which are distributed in Korea. They are classified into blue sea cucumber, red sea cucumber and black sea cucumber according to their colors.

The sea cucumbers distributed in Korea are classified into 14 species belonging to the pure sea cucumber sea turtles and the sea cucumber, and they are classified into blue sea cucumber, red sea cucumber and black sea cucumber according to their color. Results It is known that there is a genetic polymorphism in one locus, and they are treated as separate species.

Cheonghae seaweed accounts for most of the sea cucumbers produced in Korea. Chunghae is distributed more widely than red sea ginseng in rocks, small stones, sandy or sandy areas connected with pebbles except pure red areas. When I was a child, I live in mixed seaweeds and reef areas with red sea gins. It has a good flow of algae and a lot of dissolved oxygen in the sea area. It tastes less than red sea ginseng and is cheaper than black sea ginseng.

The Black Sea is mainly eel pearl, which is eutrophicated and has a lot of influence. The Black Sea is loved and loved by the Chinese people. It occupies most of the production together with the sea cucumber, and seed production is also common. The body color is very black, so it is easy to distinguish from red sea ginseng, the taste is much lower than that of red sea ginseng and its price is cheaper, but the price is higher than that of blue sea ginseng.

Red sea ginseng is a special product of Jeju area. It is mainly grown in Jeju, Gangwon, and Ulleungdo coasts. It is produced in very small amount, but has higher inorganic nutrients such as calcium, phosphorus and magnesium than blue sea cucumber. The red sea ginseng is larger than the blue sea ginseng. It is the most preferred sea cucumber of the Japanese. Especially, it is possible to live in the recording area called the desert of the sea and it is helping to recover the sea environment.

 This type of sea cucumber is mainly prepared for the production of seeds in October or November, three months before the natural spawning season. After inducing spawning by stimulation of the surface, stimulation of the surface and temperature of the water, spraying the sperm suspension And it is put on the Pentutula larvae and fed with ginseng. In general farms, more than 200 grams of seongsan will be shipped, with seed, festive, and onshore aquaculture.

The feed of the sea cucumber is mainly used in accordance with the growth stage of the sea cucumber. In the case of the Chinese couscous feed, in the case of the ginseng feed, it is composed of crude protein, crude fat, crude fiber and whey. At this time, the price of fish meal, which is used as a raw material for crude protein, has risen, and some of them have been replaced with vegetable protein such as soybean meal.

In recent years, techniques for using microalgae in aquaculture have been developed. Microalgae is a producer of organic matter from minerals using solar energy in the ocean. It is an important food for marine ecosystems such as zooplankton and fish, Respectively. Especially in aquaculture, it is used directly as feed for aquaculture such as abalone, sea cucumber, or feed for food such as altemia and rotifers, and its use is expanding.

The microalgae, which are currently being produced commercially, are mainly cultured using a photobioreactor capable of culturing at a high density. In a conventional photobioreactor, a culture medium is supplied according to the type of microalgae to be cultivated in a constant volume tank, microalgae are inoculated, and then pure culture is carried out by blocking the infestation of protozoa and molds harmful to the growth of microalgae The culture solution is filtered and sterilized.

A gas containing a carbon source such as carbon dioxide is aerated in the culture solution so that sufficient photosynthesis can be achieved, and the gas and the nutrients in the culture solution are stirred to be uniformly dispersed. For efficient photosynthesis, light of a certain wavelength is irradiated using an LED or the like depending on the characteristics of the microalgae to be cultured. Particularly, in a photoreactor, it is necessary to maintain a culture liquid at a constant temperature in order to cultivate microalgae in a large amount with a constant growth efficiency. Therefore, a heating apparatus, a heat exchanger, and the like are installed and cultivated according to the season.

Therefore, there is a problem that a large amount of energy is consumed in the mass cultivation of microalgae due to the supply and aeration of the culture medium, the irradiation of light through the light source, and the control of the water temperature. In particular, in the case of the four seasons in Korea, , There is a need for the development of a method that can be used for aquaculture.

Meanwhile, as shown in Figure 1, the Lava Seawater is seawater flowing through the basalt layer of Jeju Island and flowing into the ground underground. Lava seawater is found in some parts of western part of Jeju Island and eastern part and contains high concentration of minerals. In addition, while general seawater is exposed to unstable environment such as domestic sewage, industrial wastewater, and port pollution, it takes a lot of cost to process industrial material, while lava seawater is subjected to natural purification and filtration by volcanic rock layer to block heavy metal adsorption and harmful substances Therefore, safety, stability, and economic efficiency are ensured, and the water intake cost is inexpensive as compared with deep seawater taken from the deep sea.

The inventors of the present application confirmed that a certain amount of adherent diatoms could be obtained together with lava sea water in the process of culturing using Jeju lava seawater, and established a method for mass culture of these adherent diatoms. Adhesive diatoms identified in the present invention can be used as a feed alternative energy source for the aquaculture industry or as a valuable resource as a basis for various industrial fields such as medicine, environment, and life industry.

Korean Patent No. 10-1449557 discloses a feed composition for sea cucumber which comprises microalgae chlorella as a main component fermented by adding a microorganism to a mixture of microalgae, kelp, seaweed, parasites, rice gut, manganese, yulmu and minerals and a method for producing the same . In Korean Patent No. 10-0904983, seaweeds used as food for sea cucumbers are pulverized, and pulverized seaweeds are boiled to remove a certain amount of mucilage so that they do not adhere to each other. The seaweed feed, which can be finely ground and filtered, A manufacturing method and a manufacturing apparatus. Korean Patent No. 10-2012-0095826 discloses a method of treating carbon dioxide such as Scenedesmus sp., Chlorella sp., Spirulina sp. And the like, as well as treating biodiesel, feed additives, Discloses a photobioreactor for micro-algae high-density culture capable of realizing low-cost, high-quality, and mass-production of microalga useful in production, and a microalgae culture and harvesting method using the same. Korean Patent Registration No. 10-1256773 discloses a method for producing a wastewater treatment or biomass comprising culturing microalgae using mixed light of red light and blue light as a light source, And the amount of nitrogen and phosphorus removal can be increased. In particular, as the red light source and the blue light source, a wastewater treatment method using microscopic algae using wavelength selective LED light irradiation using a low power consumption type LED (light emitting diode) and a method for increasing productivity of microalgae biomass are disclosed. However, the above-mentioned inventions differ in the constitution and effect from the mass culturing method of the adherent microalgae of the present invention, in which the micro-prongs attaching micro-algae in natural sea water are mass-cultured and adhered and grown in direct sea cow culture.

It is necessary to establish the species selection and culture method which can be used as an excellent feed source in the aquaculture style with ease in large scale cultivation among the adherent diatoms. It is possible to identify the diatoms that are included in the lava sea water in the process of culturing using Jeju Lava Seawater and cultivate it as a dominant species and directly use it for the sea cucumber culture. By using the adventitious microalgae The purpose is to provide a method of form.

In order to solve the above-mentioned problems, the present invention provides a method for producing a water-soluble diatomaceous earth including the steps of (1) forming an adhering substrate horizontally on a bottom surface of a tank having a predetermined volume, (2) a step of culturing the adhered substrate to which the adhering substrate is attached to the outdoor water tank and culturing the adhering substrate with sunlight; (3) recovering the adhering substrate and fixing the adhering substrate vertically to the red sea gyep aquaculture tank; (4) of culturing the red sea germs using the adherent microalgae.

By massively culturing adherent microalgae from the lava water according to the present invention and using it in an environmentally friendly manner for sea cucumber cultivation, it is possible to reduce the feed cost required for the sea cucumber culture and to use it for the sea cucumber culture that supplies healthy and eco- The culture environment of sea cucumbers can be improved.

1 shows the location and general description of the distribution of lava water in Jeju Island.
Figure 2 shows the comparison of the characteristics of lava water distributed in Jeju Island with general sea water. (http://jejutp.or.kr/lavawater/whatis/what.htm)
Fig. 3 is a graph showing the distribution of the attached diatoms Achnanthes brevipes var. It is an optical microscope photograph of intermedia (A), Achnanthes sancti-pauli (B) and Melosira octogona (C).
Fig. 4 is a graph showing the distribution of Ahnanthes brevipes var. intermedia (A), Achnanthes sancti-pauli (B) and Melosira octogona (C).
FIG. 5 is a photograph showing a state in which an attachment matrix with attached diatoms attached thereto is installed in a sea cucumber culture tank.
FIG. 6 is a photograph of a sea cucumber containing an attachment matrix with adherent diatoms attached thereto.
FIG. 7 is a photograph of red sea ginseng that is adherent to diatoms at night.
FIG. 8 is a photograph showing a state in which sea cucumbers are harvested from an adhered substrate adhering to an adherent diatom. FIG.
FIG. 9 is a table showing the red sea gypsy pattern of the liquor type and circulation filtration method using lava sea water from 2013 to 2014.

In general, adherent diatoms are the primary producers of organic matter from photosynthesis and are commonly used as food organisms in aquaculture. It is mainly fed from aquaculture water and grows attached to aquaculture tank or cultivated phalanx to be fed to aquatic animals. Especially, it is a food of abalone such as abalone, seashell, bivalve such as shellfish, echinoderm such as sea cucumber, sea urchin, It is used as an early food organism when raising larvae. These adherent diatoms are mainly grown in natural seawater by attaching them to wings for attachment of aquaculture animals such as polycarbonate or polyvinyl chloride.

However, the culture of adherent diatoms is difficult to mass-produce because of the adherent nature of the culture method of conventional floating microalgae. That is, as the microalgae grow by attachment and growth, the light transmission rate by self-shading decreases, the growth of microalgae becomes stagnant, and the number of dead cells increases, and there is a problem that microalgae There is also difficulty in collecting.

On the other hand, some parts of western part of Jeju Island and the eastern part are thickly distributed from the surface of the volcanic rock formed by hardening of lava to about 150m below the sea surface. The rock layer occupies a high percentage of pores capable of storing groundwater, (Fig. 1). The Seogwipo layer under the volcanic rock is supported by the thick lava seawater layer in the volcanic rock layer as a low permeable layer and the boundary and junction between the glassy clastic breccia and the lava flows in the volcanic rock layer are composed of saltwater groundwater It is easy to make it. Jeju Lava Seawater is buried at a depth of 70m or more underground and is completely isolated from germs, barns, and hazardous chemicals, but contains a larger amount of minerals than ordinary seawater (Fig. 2).

As shown in FIG. 2, the number of general microorganisms was found to be 60,000 CFU / ml in general seawater, but was not detected in lava seawater.

In the present invention, microorganisms collected with clean lava sea water are separated and identified, and thus , Fragilariopsis sp. And Melosira nummuloides . In addition, a large-scale culture method was established to allow the adherent diatoms to be cultivated as a dominant species, and the method of isolating adherent diatoms from the lava waters of Jeju Island, Application No. 10-2016-0042700, Ahnanthes brevipes var. From the Jeju Lava Seawater was investigated . intermedia , Achnanthes sancti-pauli and Melosira octogona were further isolated and identified, and their mass culture methods and methods were established.

1. Acquisition of Adherent Diatoms from Lava Seawater

The temperature of cultured marine aquatic diatoms is 16-24 degrees and the salinity is 25 ‰ or higher. In the case of underground seawater distributed in Jeju Island, it maintains 17-18 and 25 ‰ during the year. Condition. In particular, when lava seawater is continually communicated, it is possible to maintain the water temperature change in the open hearth above 17 degrees in the winter season and below 24 degrees in the summer season. Therefore, considering that the marine- This is possible.

The inventors of the present invention have confirmed that phytoplankton can be taken together with the lava sea water during the biological test, and as a result, it has been found that Fragilariopsis sp. And Melosira nummuloides in addition to Achnanthes brevipes var. intermedia , Achnanthes sancti-pauli and Melosira octogona . The phytoplankton identified above is a species of which the industrial application is not well known in the country.

The initial lava-seawater inflow contains 2-4 cells / L of adherent diatoms. When they are attached to the 5th planes through the adherent matrix (nylon net), they are adhered to such a degree that they can be visually observed. .

Fig. 3 is a graph showing the distribution of the attached diatoms Achnanthes brevipes var. intermedia and optical micrographs of (A) and Achnanthes sancti-pauli (B) and Melosira octogona (C), Figure 4 is a scanning electron micrograph thereof. Based on the trial and error, the identified adherent tidal algae could be cultured in the following manner.

(1) Preparation of water tanks (or nogi) with microalgae

Adherence Prepare a tank in a location where it is easy to supply lava water for diatom cultivation. The water tank should have a flat bottom of 3 ~ 6 m × 5 ~ 10 m × 0.5 ~ 1.5 m in width, length and depth so that lava seawater is poured at one end of the water tank and drained at the other end so that a constant water flow is formed in the water tank do. The velocity of lava seawater is determined by the quantity of water received by the pump and the size of the tank, and is adjusted in consideration of the amount of time that the adherent gypsum can attach to the adhered substrate sufficiently. Therefore, it takes 2 to 4 rounds of lava seawater per day, and it is adjusted so that it is about 2 rotations in summer and about 4 rotations in winter.

It is preferable that a barrier membrane is laid on the hearth in order to prevent the inflow of pathogenic microorganisms from the bottom of the hearth when the adherent anchovy is adhered in the open hearth instead of the attachment bath.

(2) Attachment substrate

In order to obtain anchovy from the lava seawater, the attached substrate to which the adherent algal algae can attach is installed on the bottom of the aquarium (or the ooze). The bonding efficiency of the nylon mesh (0.1 mm) was the best for the adhered substrate. The larger the mesh size, the lower the adherence efficiency of the adherent type algae. If the mesh size is smaller than that, It was not easy.

Adhesive substrate material can be used as nylon, PP, or PE, but nylon has the highest attachment rate of sticky gypsy. The adhesive substrate spreads wide on the bottom of the attachment bath and the edge is fixed to the bottom of the wall. Or the attachment substrate may be fixed to the frame. After the adherence to the adherent substrate, the adherent adherent tyrosinase begins to multiply and oxygen generated by the photosynthetic action of the adherent adenovirus migrates into the water and collects below the bottom of the adhered substrate, causing the adherent substrate to float from the bottom surface do.

In this case, the adherent tidal algae in which the adhered substrate is exposed outside the seawater may dry out, or parasitism may propagate. Therefore, in order to prevent the adhered substrate from floating, a cross-shaped or straight-shaped incision is made in the attached substrate at an interval of 0.5 to 1 m so as to allow the gas produced by the adherent tidal flow to be discharged. A grid wire spaced 3 to 5 m apart on top of the adhered substrate prevents the adhered substrate from flooding.

When the attachment substrate is installed and the attachment substrate is attached to the attachment substrate for about 4 to 5 days, attachment of the adhesion film is completed and the attachment substrate is transferred to the culture tank for mass culture.

2. Adherence Mass culture of diatoms

(1) Attachment Attachment Species composition

Adhesive diatoms have a wide variety of diatoms, among which adherent diatoms used in abalone culture are mainly Cocconeis sp., Melosira sp. Navicula britannica , Navicula sp. Navicula closterium , Navicula longissima , Navicula marina , Navicula sp. And it is divided into a crawling type, an upright type, and a Matrix type according to the growing position, and there is a kind which has a multiplicity of growth or a colony growth, a mobility and a lack thereof, and a very fast mobility.

Adhesive diatoms obtained from Lava Seawater in Jeju Island were tested by Fragilariopsis sp. And Melosira nummuloides, in addition to Achnanthes brevipes var. intermedia, Achnanthes sancti-pauli and Melosira octogona were constantly dominant and thus favorable for establishing large-scale culture conditions of the adherent gusts obtained from lava seawater.

Achnanthes brevipes there is. intermedia And Achnanthes sancti - pauli and Melosira octogona is possible throughout the year and were incubated with the attached diatoms suitable for use in food, such as abalone, sea cucumber and excellent nutrition.

(2) Attachment Adherence  Mass culture

Lava seawater distributed in Jeju Island maintains 17-18 degrees during the year, and it is possible to maintain more than 17 degrees in winter and 24 degrees in summer. Salinity is also maintained at least 25 ‰ throughout the year, so it is possible to cultivate attached diatoms throughout the year. Table 1 shows the changes in the attached diatomaceous floatation state according to the measured daily illuminance and cloudiness in spring.

Variation of the attached diatomic flotation condition with daily illuminance and cloudiness time Illumination (Lux) Attached diatomic floating state Cloudiness Remarks 07:00 4400 - 0 08:00 22700 - 0 09:00 21100 + One 09:40 59500 ++ 0 15:00 33500 +++ 4 16:30 22300 +++ 4 17:30 9700 +++ 18:30 2700 - July 17, 2013 07:30 9500 - One 08:30 31900 + One 09:30 67200 ++ One 10:30 90100 +++ One 14:00 105100 +++ One 17:00 56400 +++ One 18:00 49500 +++ One 19:00 27200 + One

1) cloudiness; 0 (cloud 100%), 1 (80-90%), 2 (60-80%), 3 (40-60%), 4 (10-40% condition ; + (Little), ++ (many), +++ (very many)

The mass culture of the attached diatoms according to the present invention is basically based on outdoor cultivation using sunlight. In the case of Jeju Island, natural light is generally maintained at over 4,000Lux at sunrise and over 2,000Lux at sunset, and the maximum amount of light during summer is 100,000Lux. Considering that the sunrise time is 9 hours and 30 minutes for the winter time and 14 hours and 45 minutes for the winter time, sunlight is irradiated to the fine - grained diatomite more than 12 hours a day.

Considering that the life cycle of the common adhesive diatoms is up to 14 days, the life of fine algae is shortened during the summer when the growing conditions are better, and the production is increased.

3. Mass cultured adherent diatoms used in sea cucumber culture

(1) Collection of attachment matrix and composition of sea cucumber

Adherent diatoms that have grown in the adhered matrix will grow again, and if the biomass increases, the adherent matrix with adherent diatoms is recovered before it is naturally detached from the adhered matrix.

In the nylon mesh (0.1 mm) as an adherent substrate, Fragilariopsis sp., Melosira nummuloides, and Achnanthes brevipes var. intermedia, Achnanthes sancti-pauli , and Melosira octogona , which are transferred to sea cucumber culture habitat and used as bait for sea cucumbers. I have Achnanthes brevipes . intermedia And Achnanthes sancti - pauli and Melosira The octogona species increased proliferation in summer and decreased proliferation in winter, but they were able to grow continuously throughout the year. The attached substrate can be cut horizontally on the bottom of the water tank (or the ooze) to meet the water depth of the sea cucumber culture tank and installed perpendicularly from the bottom surface of the sea cucumber tank.

FIG. 5 is a photograph showing a state in which an attachment matrix with attached diatoms attached thereto is installed in a sea cucumber culture tank. The adhered substrate to which the adherent diatoms are attached is arranged to be perpendicular from the bottom surface of the sea cucumber bath. The diatoms attached to the adhered substrate are cultured with the sea cucumber culture repeatedly in the attached state. At this time, it is necessary that the adhesive diatoms absorb light uniformly to facilitate the propagation, and it is necessary that the attachment substrate is easily folded so that the attachment substrate can be easily accessed and the diatomaceous earth can be absorbed. In the present invention, the edges of the attachment substrate may be fixed to the wall surface of the sea cucumber tank or to an independently attached attachment substrate fixing device, or may be fixed to the frame so that the edges of the attachment substrate are fixed.

FIG. 6 is a photograph of a sea cucumber containing an attachment matrix with adherent diatoms attached thereto. Adhesive diatoms Achnanthes brevipes var. Intermedia and Achnanthes sancti-pauli and Melosira octogona are highly proliferated at the upper part of light transmission and increase greatly from the water surface to 70 cm, and slow from the lower 70 cm below the water surface. Therefore, the microalgae that are grown at the upper part are partially removed from the adherend surface due to the overgrowth, and moved to the lower part, and again grow in the lower part, so that a certain amount of microalgae are continuously cultured.

FIG. 7 is a photograph of red sea ginseng that is adherent to diatoms at night. A plurality of attachment substrates are arranged at regular intervals to allow a sufficient amount of diatoms to be supplied to the cultured sea cucumber. The cultured sea cucumbers stay in the lower part of the sea cucumber aquaculture under the adhered substrate in the daytime and move to the upper part of the adherent substrate in the nighttime to perform the feeding activity.

FIG. 8 is a photograph showing a state in which sea cucumbers are harvested from an adhered substrate adhering to an adherent diatom. FIG. The ginseng or the seongsam cultured for a certain period is separated from the adhered substrate by using water pressure. Adhesive diatoms Achnanthes brevipes var. Red sea ginseng fed intermedia , Achnanthes sancti- pauli , and Melosira octogona showed uniform growth, good growth rate and mortality. Ahnanthes brevipes var. intermedia , Achnanthes sancti-pauli and Melosira octogona .

(2) Experiment of sea cucumber with diatom

In order to establish a method of cultivating red sea germs using adherent microalgae, the adhered substrates with adherent diatoms acquired from lava sea water were tested for their suitability for red sea gypsy cultivation from April 2013 to October 2014. Respectively.

① Growth rate experiment

Table 2 shows the growth of red sea ginseng ( Stichopus japonicus ) ginseng using adherent diatoms. From April to June 2013, we observed the growth rate and mortality of red ginseng ginseng with an initial gross weight of 200 g. The sea cucumber cultivation was 1.0 m × 0.4 m × 0.3 m, the temperature was maintained at 15.5 ~ 17.8 ℃ and the salinity was 29.0 ~ 33.0 ‰. As shown in Table 1, the dietary intake of diatoms alone increased by more than 5 times and the mortality rate was as low as 5.26%.

Attachment Red sea germ using diatoms ( Stichopus japonicus ) Growth of ginseng date 4/14 4/22 4/29 5/6 5/13 5/22 5/27 6/3 6/6 6/9 6/17 6/24 Total population 95 95 95 95 95 95 95 92 92 92 92 90 Gross weight (g) 200 230 275 318 420 522 663 695 761 832 955 1028 Individual average weight (g) 2.1 2.4 2.9 3.4 4.4 5.5 7.0 7.6 8.3 9.0 10.4 11.4

Water temperature: 15.5 ~ 17.8 ℃, Salinity: 29.0 ~ 33.0 ‰

② Step 3  Growth rate experiment

Table 3 is a table showing the spring and summer growth of red sea ginseng ( Stichopus japonicus ) seongsan using adherent diatoms. The sea cucumber cultivation was 1.0 m × 0.4 m × 0.3 m, the water temperature was maintained at 13.0 ~ 15.0 ℃, and the salinity was 29.0 ‰. As shown in Table 2, the gross weight was about twice that of diatoms only for 43 days, and the mortality rate was 12.5%.

Attachment Red sea germ using diatoms ( Stichopus japonicus ) Spring growth of the celestial god date 3/16 3/23 4/29 Total population 40 40 35 Gross weight (g) 5440 5842 8090 Individual average weight (g) 136 ± 86.6 146 ± 92.7 231 + - 123.4

Water temperature 13.0 ~ 15.0 ℃, Salinity 29.0 ‰

Table 4 shows that the red sea ginseng ( Stichopus japonicus ) is a table showing the summer and summer growth of Sungsan. The sea cucumber cultivation was 1.0 m × 0.4 m × 0.3 m, and the water temperature was maintained at 17.0 ~ 17.5 ℃ and the salinity was 29.0 ~ 33.0 ‰. As shown in Table 3, the total weight was about twice that of diatoms only for 27 days, and the mortality rate was 12.5%.

Attachment Red sea germ using diatoms ( Stichopus japonicus ) Growing summer in the summer 5/13 5/22 2/57 6/3 6/9 Total population 10 10 10 10 10 Gross weight (g) 678.5 758.5 783.5 1006.0 1056.0 Individual average weight (g) 67.9 ± 19.5 75.9 ± 23.4 87.1 + - 39.8 111.8 + 41.5 117.3 + - 44.7

Water temperature 17.0 ~ 17.5 ℃, Salinity 29.0 ~ 33.0 ‰

(3) Lava seawater  Establishment of method of using sea cucumber

The present invention relates to a process for the production of anchovy diatoms Achnanthes brevipes var. intermedia , Achnanthes sancti- pauli , and Melosira octogona were cultured and mass cultured, and a method of breeding red sea germ using lava sea water as breeding water was established. In general, the aquacultural aquaculture can be carried out with a water-based system and a circulatory system in order to ensure a clean and well-suited aquaculture. In the case of irrigation water, if the available water resources are abundant, the natural irrigation water will be continuously fed. Depending on the natural environment, the water quality of the irrigation water can be changed. Especially, due to seasonal changes in water temperature, Is a risk factor. In addition, there is a risk of mass mortality due to pathogenic bacteria introduced from the outside, and power consumption is high in the heater for maintaining the water temperature in the winter season.

In the case of the circulation filtration, it has recently been used in the point of being able to regulate the culture conditions such as water temperature and dissolved oxygen by filtering and disinfection by forming a closed drinking water environment. However, energy is consumed to secure a constant quality of water in the breeding season according to the season, and when the aquaculture system is stopped by an unexpected accident, there is a danger that the abrupt deterioration of the quality of the breeding water leads to massive waste.

However, as shown in Fig. 1, lava seawater is naturally filtered by volcanic rock layer, and there is little organic matter and pathogens as it permeates into the underground of the land. The lava seawater maintains a constant water temperature throughout the year and maintains an average water temperature of 17 ° C during the winter It was judged that it was possible to carry out the safe water style.

FIG. 9 is a table showing the red sea gypsy pattern of the liquor type and circulation filtration method using lava sea water from 2013 to 2014. As shown in FIG. 5, red sea ginseng cultivation by the water-based method was possible in the winter when energy use increased, and it was found that the results were good in the mortality rate and the expiratory rate.

In addition, it is possible to expect a water purification function by diatoms while simultaneously cultivating adherent diatoms and sea cucumbers. By providing a culture environment similar to natural environment, it is possible to carry out the quality red sea gypsy culture.

The method of mass culturing adherent microalgae according to the present invention can adhere adherent microalgae which reproduce easily in nature and mass cultivate them and cultivated microalgae can be easily and safely used in sea cucumber culture. And thus it is industrially applicable.

Claims (4)

Jeju Island Lava Seawater is taken and exposed to the adhered substrate for a certain period of time, and adherent diatoms are attached to the adherent substrate
A method for culturing sea cucumbers using an adherent diatom, which is separated from lava waters of Jeju Island, characterized in that the adherent diatoms are cultured while culturing the adhered substrate in a sea cucumber culture aquarium.
(1) a step of horizontally installing an adherent substrate in a water tank having a constant volume and communicating the seawater of Jeju Island with lava water to attach adhesive diatoms;
(2) a step of moving the adhering substrate to which the adhesive diatoms are attached to an outdoor water tank and mass-culturing the same using sunlight;
(3) a step of culturing adherent diatoms while supplying the red sea goby to the prey and (3) culturing the adherent diatom, and recovering the adhered substrate and vertically fixing the red ginseng to the sea sea ginseng Way.
3. The method according to claim 1 or 2, wherein the adherent diatoms are selected from the group consisting of Achnanthes brevipes there is. intermedia or Achnanthes sancti - pauli or Melosira octogona . The method for culturing sea cucumbers according to the present invention is characterized in that the method comprises the steps of:
[4] The method according to claim 3, wherein the attachment substrate is a nylon net having a mesh size of 0.1 to 0.3 mm, and a criss-cross or a straight cut is formed in the net with an interval of 0.5 to 1 m. Method for the production of sea cucumber using.

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