KR20170133297A - Cosmetic composition biomimetically designed from extracellular matrix for recovering skin cell - Google Patents

Abstract

The present invention relates to a cosmetic composition biomimetically designed from an extracellular matrix for recovering skin cells, and more particularly, the present invention relates to a cosmetic composition containing effective ingredients formed of low-molecular substances for effective skin permeation in a composition ratio similar to that of the extracellular matrix. The cosmetic composition of the present invention helps the skin to recover and maintain homeostasis, and is effective for the regeneration of skin cells.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition for skin cell regeneration,

The present invention relates to a cosmetic composition for stimulating the regeneration of skin cells by simulating an extracellular matrix, and more particularly, to a cosmetic composition containing an effective ingredient having a low molecular weight in a composition ratio similar to that of an extracellular matrix . The cosmetic composition of the present invention helps the skin to recover and maintain the homeostasis, and is effective for the regeneration of skin cells.

All life is aging with age, and it goes through aging process in skin as well. Efforts to delay such aging have been constantly being carried out, and thus the question of what is the nature of aging and why aging is constantly being raised. Skin aging can be roughly divided into two types depending on the factors. One of them, intrinsic aging, is that the structure and physiological functions of the skin are constantly declining while aging, and the other, extrinsic aging, is caused by accumulated external stresses such as sunlight . Both natural aging and extrinsic aging involve structural changes in the skin, which results in dry skin, loss of skin elasticity and wrinkles.

First, the structural changes of the skin due to aging are examined in more detail. The thickness of skin, dermis, and subcutaneous tissue, which are constituents of the skin, becomes thinner. In addition, the extracellular matrix (ECM) component of the dermis, which is responsible for elasticity and tensile of the skin, changes. The extracellular matrix is a three-dimensional support containing an aqueous solution of inorganic salts, nutrients, wastes and other large polysaccharide molecules. It forms a network in the space surrounding the cells to maintain and support the morphology of the tissue, and plays an important role in regulating cell signaling and cell growth and differentiation.

The ECM components that have been simulated so far are mostly derived from animals. However, animal-derived ingredients tend not to be used in cosmetics due to safety issues such as pathogen infection.

Collagen, a major component of 70-80% of the entire extracellular matrix, is one of the longest fiber structure proteins that regulates skin strength and elasticity, and is known to be closely related to skin aging, such as elasticity and wrinkle formation. However, since most collagen currently used is an animal-derived raw material and has a molecular weight of about 300,000 Da, its absorption into collagen itself is limited in its transmission through the skin.

One of the ECM components, hyaluronic acid, binds the cells of the dermis tissue in the skin and transports the nutrients, allowing the skin to maintain an adequate amount of moisture. However, since hyaluronic acid has a molecular weight of several hundred thousand to several millions, it is highly viscous and is limited in its application amount in cosmetics, and has a drawback in that it is very difficult to penetrate skin through direct application to the skin.

Accordingly, the inventors of the present invention have found that when a low-molecular component capable of permeating skin is used to make components such as tripeptides, low molecular weight hyaluronic acid, minerals, and vitamins, which are not animal-derived components, Cell regeneration can be promoted and the present invention has been completed.

Accordingly, an object of the present invention is to provide a cosmetic composition which simulates extracellular matrix to promote regeneration of skin cells and restore the function of damaged skin.

To achieve the above object, the present invention provides a cosmetic composition comprising a tripeptide composed of glycine-proline-hydroxyproline (Gly-Pro-Hyp), a low molecular weight hyaluronic acid, a mineral component and a vitamin as an active ingredient .

According to the present invention, a cosmetic composition prepared by simulating an extracellular matrix is characterized in that a tripeptide, a low molecular weight hyaluronic acid, a mineral component and a vitamin form a network in a space surrounding the cells to maintain and support the morphology of the tissue, Can control the growth and differentiation of the skin, thereby suppressing the aging of the skin, that is, reducing the elasticity and wrinkle formation. The low-molecular components capable of permeating the skin can effectively transmit through the skin to promote skin cell regeneration Restores the function of damaged skin.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a microscope photograph showing the wound repair ability of the composition according to the present invention. FIG.
FIG. 2 is a graph showing quantitative analysis of the wound healing ability according to the concentration of the composition according to the present invention. FIG.
3 is a graph showing the effect of the composition according to the present invention on the proliferation of dermal fibroblasts.
4 is a moire image showing the skin elasticity improving effect of the composition according to the present invention.

As can be seen from FIG. 1, the cell damage area was noticeably decreased in the experimental group using the above-mentioned Example 1 as compared with the control group treated only with the DMEM medium. In addition, as can be seen from FIG. 2, it was confirmed that the relative scar recovery ability of Example 1 was superior to that of the control group, and the scar repair ability was increased in proportion to the concentration.

Accordingly, the cosmetic composition of the present invention having the extracellular matrix mimicked can promote cell regeneration and restore skin damage quickly.

[Test Example 2] Cell proliferation experiment

Fibroblasts isolated from skin of forty people were seeded in 96 wells at ³ × 10 ³ per well and cultured in M106 medium for 24 hours. Cells were cultured in 1% FBS medium for 48 hours and 72 hours, and then cultured in a cell proliferation kit (Roche kit) (FBS) supplemented with 1% fetal bovine serum (FBS) as a control and 10% FBS as a positive control. Elisa, BrdU) and observed by ELISA. The results are shown in FIG.

FIG. 3 shows that Example 1 showed almost the same proliferative effect as that of the positive control after 48 hours and significantly higher cell proliferation after 72 hours than the positive control. Therefore, the cosmetic composition mimicking the extracellular matrix of the present invention can exhibit excellent skin fibroblast proliferation effect.

[Test Example 3] Skin safety test

To examine whether allergic and primary contact dermatitis to the composition prepared in Example 1 was caused in 40 healthy adults (average age of 39.6 years, excluding skin lesion holders), 20 쨉 l of the extract of the normal person was applied to the IQ chamber and then fixed to a chamber to perform a patch test. After applying the patch for 24 hours and removing the patch, the experimental area was marked. After 30 minutes and 24 hours, the skin was stimulated by the CTFA guidelines (1981) and Frosch & Kligman (1979) The test was performed according to the criteria shown in Table 2 below.

score characteristic 0 No reaction One Mild erythema, rash or spread 2 Moderate erythema 3 Strong erythema with edema 4 Strong erythema with edema and blisters

Class I (no stimulation range): less than 1

Class II (light stimulus range): less than 3

Grade III (moderate stimulus range): less than 5

Class IV (Strong Stimulus Range): 5 or higher

As a result of the patch test, the composition of the present invention was found to be a safe cosmetic composition due to a non-stimulated stimulation score of 0 in all subjects.

[Test Example 4] Elastic effect test on human skin

A cream formulation (Example 2) containing the above Example 1 and a cream formulation (Comparative Example 1) containing no Example 1 were prepared according to the usual production method with the composition shown in the following Table 3.

ingredient Example 2
(weight%) Comparative Example 1
(weight%) Glyceryl stearate One One Pentaerythritol tetrahexyl hexanoate 5 5 Hydrogenated polydecene One One Vegetable squalane 2 2 Behenyl alcohol 6.5 6.5 Cetearyl alcohol 0.6 0.6 Phage-60 glyceryl isostearate 2.5 2.5 Dimethicone 2.5 2.5 glycerin 6 6 Butylene glycol 5 5 Active ingredient mixture 5 5 Xanthan gum 0.1 0.1 Example 1 5 - antiseptic a very small amount a very small amount Pigment a very small amount a very small amount incense a very small amount a very small amount Purified water Balance Balance

In order to measure the effect of increasing the elasticity of the skin when the cream of Example 2 was applied on the face, 22 women aged 35 to 45 years were divided into two groups. In Group 1, The other group applied Comparative Example 1 twice a day in the morning and evening. After 3 weeks and 6 weeks, facial deflection angles were measured before and after application using moiré images that can measure skin elasticity. The moiré image is shown in Fig. 4, and the measurement results of the facial deflection angle are shown in Table 4. The values in Table 4 are average values of subjects in each group.

Angle (°) 0 weeks 3 weeks 6 weeks Example 2 35.42 34.93 33.59 Comparative Example 1 36.21 35.97 35.52

As shown in Fig. 4 and Table 4, after 6 weeks of use, Comparative Example 1 exhibited a reduction of facial deflection angle of 1.91% and Example 2 decreased 5.17%. It was found that Example 1 more effectively works to improve the facial deflection angle, and it is confirmed that the composition of the present invention has an effect of increasing skin elasticity.

[Test Example 5] Skin tone improvement experiment

When the function of the skin cells is restored and the regeneration is promoted, the uneven skin tone is uniformly improved. In order to confirm the improvement of skin tone, 22 women aged 35 to 45 years were divided into two groups. One group was applied to Example 2 and the other group was applied to Comparative Example 1 twice a day in the morning and evening. After 3 weeks and 6 weeks, facial stage DM-3 (Moritex, Japan) was used to evaluate the improvement of skin tone. The non-uniformity of skin brightness was measured and the results are shown in Table 5 below.

Unevenness of skin tone (%) 0 weeks 3 weeks 6 weeks Example 2 8.14 8.07 7.81 Comparative Example 1 8.12 8.10 8.03

As shown in Table 5, the unevenness of skin tone was reduced by 4.05% in Example 2, and by 1.11% in Comparative Example 1 in the evaluation after 6 weeks. Thus, it was confirmed that the composition according to the present invention is effective in uniformizing skin tone.

[Formulation Example 1] Flexible longevity

The formulated softening water containing the above Example 1 was prepared according to the usual production method with the composition shown in Table 6 below.

ingredient Content (% by weight) Betaine 3.0 Natto sword 3.0 Cellulose sword 0.005 ethanol 10.0 Polyoxyethylene hardened castor oil 0.2 Tocopheryl acetate 2.0 Example 1 10 antiseptic a very small amount Pigment a very small amount incense a very small amount Purified water Balance

[Formulation Example 2] Nutritional lotion

A nutritional lotion formulation containing the above Example 1 was prepared according to a conventional preparation method with the composition shown in Table 7 below.

ingredient Content (% by weight) Cetyl ethylene hexanoate 4.0 Cetostearyl alcohol 1.0 Chin type monostearyl stearate 1.0 Squalane 0.5 Example 1 5.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 glycerin 5.0 Triethanolamine 0.5 Carboxyvinyl polymer 0.2 antiseptic a very small amount Pigment a very small amount incense a very small amount Purified water Balance

[Formulation Example 3] Cream

The cream formulations containing Example 1 were prepared according to the usual preparation methods with the compositions shown in Table 8 below.

ingredient Content (% by weight) Glyceryl stearate 3.0 Polysorbate 60 1.0 Cetostearate 2.5 Wax 1.0 Squalane 2.0 Sorbitan sesquioleate 0.5 Cetyl ethyl hexanoate 0.5 Liquid paraffin 5.0 Active ingredient mixture 5.0 glycerin 3.0 Propylene glycol 3.0 Example 1 5.0 antiseptic a very small amount Pigment a very small amount incense a very small amount Purified water Balance

Claims (8)

A tripeptide comprising glycine-proline-hydroxyproline (Gly-Pro-Hyp), a low molecular weight hyaluronic acid, a mineral component and a vitamin as active ingredients,
Wherein the low molecular weight hyaluronic acid has a molecular weight of 500 to 5000 Da. The cosmetic composition according to claim 1, wherein the mixing ratio of the tripeptide and the low molecular weight hyaluronic acid is 1: 1 to 1: 2 by weight. The cosmetic composition according to claim 1, wherein the tripeptide is contained in an amount of 0.001 to 3% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the tripeptide and the low molecular weight hyaluronic acid are mixed at a weight ratio of 1: 2. The cosmetic composition according to claim 1, wherein the mineral component is at least one selected from the group consisting of magnesium chloride, calcium chloride, potassium chloride, magnesium fluoride, manganese fluoride, manganese sulfate, zinc sulfate, magnesium sulfate, . The cosmetic composition according to claim 1, wherein the mineral component is contained in an amount of 0.001 to 3% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the vitamin is at least one selected from the group consisting of inositol, ascorbic acid, pantothenic acid, niacin, folic acid, thiamine, riboflavin and niacinamide. The cosmetic composition according to claim 1, wherein the vitamin is contained in an amount of 0.001 to 3% by weight based on the total weight of the composition.

Images