WO2022169246A1 - Cosmetic material composition - Google Patents

Abstract

The present invention relates to a cosmetic material composition which is safe for the human body, improves the condition of the skin, and has cosmetic effects, by comprising, as an active ingredient, at least one type selected from the group consisting of thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, carnosine, and pharmaceutically acceptable salts thereof.

Description

cosmetic composition

The present invention relates to a cosmetic composition comprising thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, carnosine, and pharmaceutically acceptable salts thereof as active ingredients. .

The skin is the organ that occupies the largest area in the human body and plays a role in protecting the human body from the outside, and the skin barrier can be divided into a physical barrier, a chemical barrier, and an immune barrier according to its function. The physical barrier is a tight junction that attaches the gaps between skin cells, filling the gaps between cells and preventing harmful substances from entering. Chemical barriers are created when pH conditions are changed by substances secreted by cells or components such as antibacterial peptides, natural moisturizing factors, and free fatty acids are secreted to prevent harmful substances from entering. For example, antibacterial peptides (Defensin, LL-37, Psoriacin, etc.) generated from skin cells and flora can participate in primary immunity by removing bacteria and viruses introduced from the outside. The immune barrier is a barrier made by immune cells such as Langerhans cells, macrophages, and T cells that exist in the skin, and provides a defense mechanism against harmful substances that have passed through the physical and chemical barriers.

In recent years, as living standards improve, various cosmetic compositions for skin health management, such as improvement of skin barrier, have been provided in addition to efforts to maintain beautiful skin. The cosmetic composition may contain an active ingredient that imparts a condition improving effect such as anti-aging and skin protection and a cosmetic effect such as moisturizing and whitening. Conventionally, research has been actively conducted to reduce the side effects of cosmetic compositions by identifying and applying metabolites or extracts derived from natural materials such as microorganisms and plants as active ingredients. Recently, not only natural substances, but also substances known to exhibit a predetermined effect in fields such as medicines and health functional foods, as active ingredients in cosmetic compositions, have been studied.

Therefore, as substances that have been administered into the human body by various methods other than conventional application to the skin to obtain a predetermined effect, research on compositions that are safe for the human body and that can effectively improve the condition of the skin or beautify the skin are attracting attention. the current situation.

The present invention was created to solve the problems of the prior art as described above, and an object of the present invention is thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, carnosine and It is to provide a cosmetic composition comprising at least one selected from the group consisting of pharmaceutically acceptable salts thereof as an active ingredient.

The cosmetic composition according to the present invention comprises at least one selected from the group consisting of thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol and carnosine as an active ingredient. characterized.

More specifically, the cosmetic composition according to the present invention contains thiamine, pyridoxine, folic acid, cyanocobalamin, glycyletinic acid, aluminum sucrose octasulfate, lupeol and carnosine and pharmaceutically acceptable salts thereof as active ingredients. may include

In the present invention, "cosmetic composition" means to deliver the active ingredient included in the composition to the skin and skin cells constituting the skin by applying it to the skin.

In the present invention, the cosmetic composition may mean that it is provided for improving the condition of the skin, moisturizing the skin, or improving the skin barrier.

Specifically, the improvement of the skin condition can mean including increasing the activity and proliferation of skin cells such as dermal cells, increasing the amount of collagen synthesis in the skin cells, and reducing the amount of melanin synthesis in the skin cells. have. For example, the improvement of the skin condition is to improve the regeneration action of the skin through the activation and proliferation of skin cells, to increase the collagen synthesis ability of the skin cells to improve wrinkles or elasticity of the skin, and to synthesize melanin in the melanocytes By suppressing it, it may mean including providing a whitening or brightening effect to the skin. Skin whitening may mean whitening the skin through inhibition of melanin synthesis, and skin brightening may mean brightening dark skin through skin keratin management or normalization of oil-water balance. can The cosmetic composition of the present invention can provide a cosmetic effect by preventing the occurrence of skin blemishes and deterioration of elasticity through skin improvement.

Specifically, moisturizing the skin may mean increasing the expression rate of hyaluronic acid synthase in the skin cells, increasing the amount of hyaluronic acid synthesis in the skin cells, and increasing the moisture content in the skin cells. The cosmetic composition of the present invention can provide an anti-aging effect through skin moisturizing.

Specifically, improvement of the skin barrier means increasing the amount of antimicrobial peptide synthesis in skin cells, increasing the amount of collagen type 7 synthesis in skin cells, and increasing the expression level of antioxidant protein in skin cells. can The skin is exposed to oxidative stress every day and is prone to injuries. The cosmetic composition of the present invention improves antioxidant activity by increasing the expression level of antioxidant protein, By increasing the amount of synthesis, it is possible to provide an effect of improving the skin barrier by improving the regeneration action.

The cosmetic composition according to an embodiment of the present invention improves skin regeneration, increases skin cell proliferation, improves skin wrinkles or elasticity, increases collagen synthesis in skin cells, moisturizes skin, and increases hyaluronic acid synthase in skin cells. Increased expression rate, skin whitening or brightening, inhibition of melanin synthesis in melanocytes, improvement of skin barrier, increased production of antibacterial peptides in skin cells, increased synthesis of collagen 7 in skin cells, or increased expression of antioxidant proteins in skin cells use can be provided.

In the present invention, "active ingredient" means a substance or group of substances that imparts a beneficial effect on any one or more of skin condition, health and beauty.

In the present invention, "application" of an active ingredient means delivering the active ingredient to skin cells through application of the cosmetic composition to the skin by one or more methods of active transport, diffusion, and delivery through a transport medium.

Specifically, in the present invention, the active ingredient may be at least one vitamin B selected from the group consisting of thiamine, pyridoxine, folic acid and cyanocobalamin. Vitamin B is a water-soluble vitamin that acts as an enzyme as a cofactor for metabolism in cells and is used as a precursor for making enzymes. B vitamins are mainly absorbed through oral administration and used for cellular metabolism and immune response.

Thiamine, also called vitamin B1, has a structure in which aminopyrimidine and thiazolium are connected. Thiamine is generally absorbed through oral administration and is involved in carbohydrate metabolism and synthesis of nucleic acids in the human body.

When thiamine and a pharmaceutically acceptable salt of thiamine are applied to the skin, the cell proliferation capacity of the skin, the collagen synthesis ability of the skin cells, the moisture content of the skin cells, the amount of collagen 7 synthesis in the skin cells and the production of antibacterial peptides are increased. , has the effect of inhibiting the synthesis of melanin in melanocytes. Thiamine in the present invention may be obtained from grains, legumes, meat, fruits or yeast, but may also be purchased and used in domestic and foreign markets.

Pyridoxine, also called vitamin B6 or hydroxymethylpyridine, is absorbed through oral administration and used for glycogen metabolism, amino acid synthesis, and prevention and treatment of anemia in the human body.

When pyridoxine and a pharmaceutically acceptable salt of pyridoxine are applied to the skin, it increases the cell proliferation ability of the skin, the collagen synthesis ability of the skin cells, the moisture content of the skin cells, the amount of collagen 7 synthesis in the skin cells, and the production of antibacterial peptides. , has the effect of inhibiting the synthesis of melanin in melanocytes. In the present invention, pyridoxine can be obtained from grains, vegetables, and fruits, but can also be purchased and used commercially at home and abroad.

Folic acid, also called vitamin B9, folic acid, or folic acid, has a structure in which 2-amino-4-hydroxypteridine, p-aminobenzoic acid, and polyglutamate are linked. Folic acid is absorbed through oral administration and is used for nucleic acid synthesis and activation of vitamin B12 in the human body.

When folic acid and pharmaceutically acceptable salts of folic acid are applied to the skin, it increases the cell proliferation ability of the skin, the collagen synthesis ability of the skin cells, the moisture content of the skin cells, the amount of collagen 7 synthesis in the skin cells, and the production of antibacterial peptides. , has the effect of inhibiting the synthesis of melanin in melanocytes. In the present invention, folic acid can be obtained from grains, vegetables, and fruits, but can also be purchased and used in domestic and overseas markets.

Cyanocobalamin, also called vitamin B12 or cyanocobalamin, is a coordination compound containing cobalt. Cyanocobalamin is absorbed through oral administration and used for the prevention and treatment of anemia in the human body.

When cyanocobalamin and a pharmaceutically acceptable salt of cyanocobalamin are applied to the skin, the cell proliferation ability of the skin, the collagen synthesis ability of the skin cells, the moisture content of the skin cells and the production of antibacterial peptides are increased, and the melanin of the melanocytes is increased. It has the effect of inhibiting synthesis. In the present invention, cyanocobalamin can be obtained by fermentation of microorganisms, but can also be purchased and used in domestic and foreign markets. Specifically, another active ingredient of the present invention may be one or more selected from the group consisting of glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, and carnosine.

Glycyrrhetinic acid, also called enoxolone, is a pentacyclic triterpenoid aglycone metabolite of glycyrrhizine. Glycyretinic Acid is absorbed through oral administration and used to improve digestion and metabolize prostaglandins in the human body.

When glycyrrhetinic acid and a pharmaceutically acceptable salt of glycyrrhetinic acid are applied to the skin, the collagen synthesis ability of skin cells, the amount of collagen 7 synthesized in the skin cells, and the production of antibacterial peptides in the skin cells has the effect of increasing In the present invention, glycyrrhetinic acid can be obtained from plants such as licorice, but can also be purchased and used in domestic and foreign markets.

Aluminum sucrose octasulfate (aluminum sucrose octasulfate) is a sucrose complex of aluminum hydroxyaluminum, and it is absorbed through oral administration and used to prevent ulcers in the human body.

When aluminum sucrose octasulfate and pharmaceutically acceptable salts of aluminum sucrose octasulfate are applied to the skin, there is an effect of increasing the collagen synthesis ability of the skin cells, the moisture content of the skin cells, and the production of antibacterial peptides. Aluminum sucrose octasulfate can be obtained by reacting an ammonium salt of sucrose octasulfate with aluminum chloride, but it can also be purchased and used commercially at home and abroad.

Lupeol (lupeol) is a type of triterpene, absorbed through oral administration and used for anti-tumor and cancer prevention in the human body.

When lupeol and a pharmaceutically acceptable salt of lupeol are applied to the skin, it increases the cell proliferation ability of the skin, the collagen synthesis ability of the skin cells, the moisture content of the skin cells, and the production of antibacterial peptides, and improves the melanin synthesis of melanocytes. has a deterrent effect. In the present invention, lupeol may be obtained from plants, but may be purchased and used commercially at home and abroad.

Carnosine, also called beta-alanyl-L-histidine, has a structure in which beta-alanine and L-histidine are linked. Carnosine is known to act as a buffer for muscles.

When carnosine and a pharmaceutically acceptable salt of carnosine are applied to the skin, the cell proliferation capacity of the skin, the moisture content of the skin cells, the amount of collagen 7 synthesis in the skin cells, the production of antibacterial peptides and the expression of antioxidant proteins in the skin cells It increases the amount and has the effect of inhibiting melanin synthesis in melanocytes. In the present invention, carnosine can be obtained from meat, but can also be purchased and used in domestic and foreign markets.

The cosmetic composition according to an embodiment of the present invention is a group consisting of thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, carnosine, and pharmaceutically acceptable salts thereof. It may contain one or more selected from the group as an active ingredient, and when containing two or more kinds from the group as an active ingredient, each component may be included in the same ratio.

The cosmetic composition according to an embodiment of the present invention may include the active ingredient in an amount of 0.000001 to 10% by weight, respectively, based on 100% by weight of the total. Preferably, the active ingredient may be included in an amount of 0.00001 to 1% by weight based on 100% by weight of the total cosmetic composition.

Alternatively, the cosmetic composition may include each of the active ingredients in an amount of 0.01 ppm to 100,000 ppm based on the total weight. Preferably, the active ingredient may be included in an amount of 0.01 to 10,000 ppm based on the total weight of the cosmetic composition. Hereinafter, the concentration of the active ingredient is also applied to the pharmaceutically acceptable salt of each ingredient.

For example, based on the total weight of the cosmetic composition, thiamine may be included in an amount of 100,000 ppm or less. In order to obtain a significant effect on promoting skin cell activity, it is preferable to include thiamine in an amount of 10,000 ppm or less, and most preferably, it may include 1,000 ppm or less. In order to obtain a significant effect on increasing the amount of collagen synthesis in skin cells, antibacterial peptide synthesis and collagen type 7 synthesis in skin cells, it is preferable to contain thiamine in an amount of 1,000 ppm or less, and most preferably, it contains 100 ppm or less. can In order to obtain a significant effect on the expression level of hyaluronic acid synthase in skin cells, thiamine is preferably included in an amount of 100,000 ppm or less, and most preferably, it may be included in an amount of 10,000 ppm or less. In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include thiamine in an amount of 1 ppm or more, more preferably 5 ppm or more, and most preferably 10 ppm or more.

For example, based on the total weight of the cosmetic composition, pyridoxine may be included in an amount of 10,000 ppm or less. In order to obtain a significant effect for enhancing skin cell activity, increasing the amount of collagen synthesis in skin cells and increasing the amount of antibacterial peptide, it is preferable to contain pyridoxine at 1,000 ppm or less, and most preferably, it may contain 100 ppm or less. In order to obtain a significant effect on the expression level of hyaluronic acid synthase in skin cells, it is preferable to include pyridoxine in an amount of 10,000 ppm or less, and most preferably, it may include 1,000 ppm or less. In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include pyridoxine in an amount of 1 ppm or more, more preferably 10 ppm or more, and most preferably 100 ppm or more. In order to obtain a significant effect on increasing the amount of collagen type 7 synthesis in skin cells, pyridoxine is preferably included in an amount of 100 ppm or less, and most preferably, it may be included in an amount of 10 ppm or less.

For example, based on the total weight of the cosmetic composition, folic acid may be included in an amount of 1,000 ppm or less. In order to obtain a significant effect on promoting skin cell activity, folic acid may be included in an amount of 100 ppm or less, more preferably 10 ppm or less, and most preferably 1 ppm or less. In order to obtain a significant effect on increasing the amount of collagen synthesis in skin cells, folic acid is preferably included in an amount of 10 ppm or more, and most preferably, it may be included in an amount of 100 ppm or more. In order to obtain a significant effect on the expression level of hyaluronic acid synthase in skin cells, folic acid is preferably included in an amount of 100 ppm or less, and most preferably, it may be included in an amount of 10 ppm or less. In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include folic acid in an amount of 10 ppm or more, and most preferably, it may include 100 ppm or more. In order to obtain a significant effect on increasing the amount of antibacterial peptide synthesis and collagen type 7 synthesis in skin cells, it is preferable to include folic acid in an amount of 100 ppm or less, and most preferably, it may include 10 ppm or less.

For example, based on the total weight of the cosmetic composition, cyanocobalamin may be included in an amount of 1,000 ppm or less. In order to obtain a significant effect on promoting skin cell activity, it is preferable to include cyanocobalamin in an amount of 100 ppm or less, more preferably 10 ppm or less, and most preferably 1 ppm or less. In order to obtain a significant effect on increasing the amount of collagen synthesis in skin cells, the expression level of hyaluronic acid synthase in the skin cells, and the amount of antibacterial peptide synthesis, the content of cyanocobalamin is preferably 1,000 ppm or less, and most preferably 100 ppm or less. can be included as In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include cyanocobalamin in an amount of 1 ppm or more, and most preferably, it may include 10 ppm or more.

For example, based on the total weight of the cosmetic composition, glycyrrhetinic acid may be included in an amount of 100 ppm or less. In order to obtain a significant effect on increasing the amount of collagen synthesis in skin cells, it is preferable to include glycyrrhetinic acid in an amount of 10 ppm or less, more preferably 1 ppm or less, and most preferably 0.1 ppm or less. . In order to obtain a significant effect on increasing the amount of antibacterial peptide synthesis and collagen type 7 synthesis in skin cells, it is preferable to include glycyrrhetinic acid in an amount of 10 ppm or less, more preferably 5 ppm or less, most preferably 1 It may contain less than ppm.

For example, based on the total weight of the cosmetic composition, aluminum sucrose octasulfate may be included in an amount of 100 ppm or less. In order to obtain a significant effect on increasing the amount of collagen synthesis in skin cells and expression of hyaluronic acid synthase in skin cells, it is preferable to contain aluminum sucrose octasulfate at 100 ppm or less, and most preferably, it contains 10 ppm or less. can In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include 100 ppm or less of aluminum sucrose octasulfate, and most preferably, it may include 10 ppm or less. In order to obtain a significant effect on increasing the amount of antibacterial peptide synthesis, aluminum sucrose octasulfate may be included in an amount of 100 ppm or less.

For example, based on the total weight of the cosmetic composition, lupeol may be included in an amount of 100 ppm or less. In order to obtain a significant effect on promoting skin cell activity, it is preferable to include lupeol in an amount of 50 ppm or less, and most preferably, it may include 5 ppm or less. In order to obtain a significant effect on increasing the amount of collagen synthesis in skin cells, it is preferable to include lupeol in an amount of 10 ppm or less, more preferably 1 ppm or less, and most preferably 0.1 ppm or less. In order to obtain a significant effect on the expression level of hyaluronic acid synthase in skin cells, it is preferable to include lupeol in an amount of 10 ppm or less, more preferably 5 ppm or less, and most preferably 1 ppm or less. In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include lupeol in an amount of 10 ppm or less, more preferably 5 ppm or less, and most preferably 1 ppm or less. In order to obtain a significant effect on increasing the amount of antibacterial peptide synthesis, lupeol may be included in an amount of 100 ppm or less.

For example, based on the total weight of the cosmetic composition, carnosine may be included in an amount of 100,000 ppm or less. In order to obtain a significant effect on promoting skin cell activity, it is preferable to include carnosine in an amount of 100,000 ppm or less, and most preferably, it may include 10,000 ppm or less. In order to obtain a significant effect on increasing the expression level of hyaluronic acid synthase in skin cells and the amount of antibacterial peptide synthesis, it is preferable to include carnosine in an amount of 1,000 ppm or less, and most preferably, it may include 100 ppm or less. In order to obtain a significant effect on the reduction of melanin production of melanocytes, it is preferable to include 100 ppm or more of carnosine, and most preferably, it may include 1,000 ppm or more. In order to obtain a significant effect on increasing the amount of collagen type 7 synthesis in skin cells, it is preferable to include carnosine in an amount of 1,000 ppm or less, more preferably 100 ppm or less, and most preferably 10 ppm or less. In order to obtain a significant effect on increasing the expression of antioxidant protein in skin cells, it is preferable to include carnosine in an amount of 200 ppm or less, and most preferably, it may include 20 ppm or less.

As used herein, the term "cosmetic composition" refers to a composition for applying an active ingredient included in the composition to skin cells by directly applying or spraying it to the skin.

The cosmetic composition according to the present invention is a lotion, facial lotion, body lotion, nourishing cream, moisture cream, eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, cleansing cream, cleansing lotion , cleansing oil, cleansing foam, soap or body wash formulation.

The cosmetic composition according to the present invention may be for use on the scalp in the skin. In this case, the cosmetic composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nourishing lotion, hair shampoo, hair conditioner, hair treatment, hair Cream, hair nourishment cream, hair moisture cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nourishment pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, wave agent for hair, hair It may be in the form of a bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair mousse or hair spray.

When the composition of the present invention is formulated in a liquid form, the composition of the present invention may include a carrier for the active ingredient. The carrier is, for example, water, ethanol, castor oil, glycerin, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbate. It may be a fatty acid ester such as hearth, but is not limited thereto. These may be used alone or in combination of two or more.

When the composition of the present invention is formulated as a paste, cream or gel, the composition of the present invention may include a carrier for the active ingredient. The carrier is, for example, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivatives such as tracanth, hydroxyethyl, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, cetostearyl alcohol or chloride It may be stearyltriethylammonium, but is not limited thereto. These may be used alone or in combination of two or more.

When the composition of the present invention is formulated as a powder or spray, the composition of the present invention may include a carrier for the active ingredient. The carrier may be, for example, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, but is not limited thereto. These may be used alone or in combination of two or more. When the composition of the present invention is formulated as a spray, a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether may be further included.

When the composition of the present invention is formulated as a soap, the composition of the present invention may include a carrier for the active ingredient. The carrier may be, for example, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc., but is limited thereto. it's not going to be These may be used alone or in combination of two or more.

The composition of the present invention may further include additives commonly used for cosmetic compositions. The additive may be, for example, purified water, a surfactant, a moisturizer, a lower alcohol, a chelating agent, a disinfectant, an antioxidant, a preservative, a color, or a fragrance, but is not limited thereto. These may be used alone or in combination of two or more.

Above, all components included in the present invention do not exceed the regulations set by each country. Preferably, the cosmetic composition of the present invention may include the individual components mentioned within a range that does not exceed the maximum amount stipulated in the "Cosmetics Safety and Technical Code" set by the Chinese government.

The skin improvement method according to an embodiment of the present invention includes thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, carnosine, and pharmaceutically acceptable salts thereof. It comprises the step of treating the skin with a cosmetic composition containing at least one selected from the group consisting of as an active ingredient.

The skin improvement method is for improving the regeneration action of the skin, increasing the cell proliferation ability of the skin, improving wrinkles or elasticity of the skin, increasing the collagen synthesis ability of the skin cells, or for moisturizing the skin, Increase the expression rate of hyaluronic acid synthase in skin cells, for whitening or brightening the skin, inhibiting melanin synthesis in melanocytes, improving the skin barrier, increasing the production of antibacterial peptides in skin cells, or skin It is possible to increase the amount of collagen 7 synthesis in cells, or increase the expression level of antioxidant protein in skin cells.

The skin improvement method according to an embodiment of the present invention includes applying the cosmetic composition to a lotion, facial lotion, body lotion, nutrition cream, moisture cream, eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, and makeup base. , foundation, powder, cleansing cream, cleansing lotion, cleansing oil, cleansing foam, soap or body wash formulation.

The method for improving skin according to an embodiment of the present invention includes applying the cosmetic composition to a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, Hair Moisture Cream, Hair Massage Cream, Hair Wax, Hair Aerosol, Hair Pack, Hair Nutrient Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Drying Agent, Hair Preservative, Hair Dyeing Agent, Hair Wave Agent, Hair Bleaching Agent, Hair Gel, Hair It includes the step of treating the scalp in the form of a glaze, a hair dresser, a hair lacquer, a hair moisturizer, a hair mousse or a hair spray.

The skin improvement method includes a skin condition improvement method, a skin moisturizing method, and a skin barrier improvement method.

The cosmetic composition according to the present invention is harmless to the human body, improves the condition of the skin, provides a skin moisturizing effect, and can impart an effect of improving the skin barrier.

Specifically, the cosmetic composition according to the present invention can increase the proliferative ability of skin cells. Accordingly, the activity of skin cells is increased to give vitality to the skin and provide a lively effect.

Specifically, the cosmetic composition according to the present invention can increase the collagen synthesis ability of skin cells. Accordingly, the elasticity of skin cells is increased to provide an effect of preventing aging.

Specifically, the cosmetic composition according to the present invention can increase the expression rate of hyaluronic acid synthase in skin cells. Accordingly, the moisture content in the skin cells is increased, providing an effect of moisturizing and providing elasticity to the skin.

Specifically, the cosmetic composition according to the present invention can inhibit melanin synthesis in melanocytes in the skin. Accordingly, it provides a whitening effect on the skin.

Specifically, the cosmetic composition according to the present invention increases the production of antibacterial peptides in skin cells. Accordingly, by improving the immunity of the skin, it provides the effect of protecting the skin and preventing inflammation.

Specifically, the cosmetic composition according to the present invention increases the amount of collagen 7 synthesis in skin cells. Accordingly, it provides a protective effect through the structure and maintenance of the skin barrier by improving the structural retention and wound regeneration of the epidermis and dermis.

Specifically, the cosmetic composition according to the present invention increases the expression level of antioxidant protein in skin cells. Accordingly, by removing active oxygen from the skin exposed to the external environment first in the human body, it protects the skin from oxidation and provides a protective effect through the construction and maintenance of the skin barrier.

1 is an image showing the results of Western blot to confirm the expression of carnosine SPRR1a as an active ingredient according to the present invention.

The objects, specific advantages and novel features of the present invention will become more apparent from the following detailed description and preferred embodiments. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples. In addition, in describing the present invention, if it is determined that a detailed description of a related known technology may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

Experimental Example 1. Efficacy evaluation on skin cell activity by active ingredient

Thiamine (Sigma Aldrich, T4625-100g), pyridoxine (Sigma Aldrich, PHR1036-500MG), folic acid (Sigma Aldrich, F8758-5G), cyanocobalamin (Sigma aldrich, V2876-5g), glycyreti used in the following experimental examples Nick acid (Sigma Aldrich, E0180500), aluminum sucrose octasulfate (Sigma Aldrich, Y0001324), lupeol (Sigma Aldrich, L5632-100mg), carnosine (Sigma Aldrich, C9625-5G) and their pharmaceutically acceptable As the salt, a commercially available product was used.

In order to evaluate the effect of each active ingredient on the activity and proliferation of skin cells, the color development of formazan generated by the decomposition of tetrazolium salt was confirmed using Cell Counting Kit-8 (CCK8, Dojindo, Japan). Since cells with active and proliferative activity actively produce energy in mitochondria, the dehydrogenase used for energy production decomposes tetrazolium salts to produce formazan. For color development, absorbance was measured at 450 nm with an ELISA Reader (Epoch, Bio-tech Instrument INC., USA).

After inoculating human foreskin-derived human fibroblasts (dermal cells, HS68) into the bottom of a culture dish, penicillin (100 IU/ml), streptomycin (100 μg/ml) (GibcoBRL, USA) and 10% fetal bovine serum ( Dulbecco's modified eagles medium (DMEM, GibcoBRL, USA) containing fetal bovine serum; FBS, GibcoBRL, USA) was added and cultured at 37°C in an incubator (Forma Scientific, France) containing 5% carbon dioxide. When the fibroblasts reached about 60 to 70% of the surface of the culture dish, the cells were recovered by treatment with 0.25% trypsin (Gibco BRL, USA) and cultured. Each well of a 96-well culture plate (Falcon Co) 1 x 10 ⁴ cells were dispensed per sugar and cultured for 1 day. After culturing for one day, each active ingredient was mixed with DMEM according to the experimental concentration in Table 1 below, put into each well, and incubated for 24 hours again, after which 10 μl of CCK-8 solution was added to each well, % CO ₂ was reacted for 1 hour in a donated cell incubator. And absorbance was measured at 450 nm by ELISA reade. The experiment was performed in the same three wells for each concentration, and the results were averaged and shown in Table 1. At this time, cell activity was compared with a relative value to a positive control treated with FBS at a concentration of 3%, and the degree of increase or decrease was expressed as a percentage.

active ingredient	treatment concentration	Cell activity sensitivity (%)
Thiamine	100 ppm	3.5
	1,000 ppm	23.7
pyridoxine	100 ppm	75.4
folic acid	1 ppm	21.2
Cyanocobalamin	1 ppm	24
lupeol	0.01 ppm	30.9
	0.1 ppm	55.8
	1 ppm	73.2
	5 ppm	87.3
carnosine	1,000 ppm	34.7
	10,000 ppm	126

When thiamine, pyridoxine, folic acid, cyanocobalamin, lupeol and carnosine were each used as active ingredients, although there was a difference in the treatment concentration for each active ingredient, the proliferation of dermal cells was found to be significant.

Experimental Example 2. Efficacy evaluation on collagen synthesis of skin cells by active ingredient

The effect of each active ingredient on collagen synthesis in skin cells was evaluated. As dermal cells, HS68 capable of collagen synthesis was used, and PICP (Procollagen Type I C-peptide) EIA assay was used. Type I precursor collagen C-terminal peptide (PICP), which is typically synthesized the most in fibroblasts, was quantitatively measured. 1.5 x 10 ⁵ cells were dispensed per well in a 24-well culture plate and cultured for 24 hours. Each active ingredient was added to DMEM without serum, and treated for 48 hours. 20 μl of the culture supernatant was taken and used for collagen synthesis measurement.

The amount of type I procollagen C-terminal peptide was measured using the PICP EIA kit (Takara shuzo, Japan, Cat. # MK101). The enzymatic immunoassay is a one-step sandwich method. A 96-well culture plate is coated with a mouse monoclonal antibody against PIP (procollagen I peptide), and 20 μl of a cell culture medium at 37°C for 3 hours and an anti-mouse PIP monoclonal antibody labeled with peroxidase. reacted with H ₂ O ₂ and tetramethylbenzidine, and 100 μl of 1N sulfuric acid was added to terminate the reaction. The absorbance was measured at the wavelength. Quantitative measurements were made by making a graph with the PICP standard concentration.

The treatment concentration for each active ingredient is as shown in Table 2 below, and the degree of increase or decrease is expressed as a percentage by comparison with a control group that is not treated with anything.

active ingredient	treatment concentration	Collagen synthesis (%)
Thiamine	100 ppm	14.3
pyridoxine	100 ppm	4.7
folic acid	100 ppm	14.4
Cyanocobalamin	100 ppm	8.8
Glycyretinic Acid	0.1 ppm	14
aluminum sucrose Octasulfate	10 ppm	8.8
lupeol	0.1 ppm	8.3

When thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate and lupeol were used as active ingredients, although there was a difference in the treatment concentration for each active ingredient, the increase in collagen synthesis was found to be significant. In the case of thiamine at a concentration above a certain concentration and in the case of folic acid at a concentration below a certain concentration, it was found that collagen synthesis was reduced.

Experimental Example 3. Efficacy evaluation on the expression of hyaluronic acid synthase in skin cells by active ingredient

The effect of each active ingredient on the expression of hyaluronic acid synthase in skin cells was evaluated. Hyaluronic acid is a substance that has an important influence on the moisture content of cells by keeping the cells in a state of absorbing moisture in the skin, and the expression level of HAS3 (hyaluronan synthase 3), an enzyme required for the synthesis of hyaluronic acid, was confirmed.

HaCaT cells were used as skin cells, and Dulbecco's modified containing penicillin (100 IU/ml), streptomycin (100 μg/ml) (GibcoBRL, USA) and 10% fetal bovine serum (FBS, GibcoBRL, USA). eagles medium (DMEM, GibcoBRL, USA) was added and cultured in an incubator (Forma Scientific, France) containing 5% carbon dioxide at 37°C. 1 x 10 ⁶ cells were dispensed per well in a 24-well culture plate and cultured for 24 hours. Herein, each active ingredient was added to DMEM without serum and treated for 72 hours. RNA was extracted from the cells and the expression level of HAS3 was confirmed through qPCR (Qiagen Rnase mini kit).

The treatment concentration for each active ingredient is as shown in Table 3 below, and the relative expression level of the control group that was not treated with anything was set to 1.00 and expressed as a relative value.

active ingredient	treatment concentration	Relative mRNA expression
Thiamine	100 ppm	1.37
	10,000 ppm	2.74
pyridoxine	100 ppm	1.49
	1,000 ppm	1.80
folic acid	1 ppm	1.07
	10 ppm	1.22
Cyanocobalamin	1 ppm	1.18
	100 ppm	1.37
aluminum sucrose Octasulfate	10 ppm	23.7
lupeol	1 ppm	1.10
	5 ppm	1.50
	10 ppm	2.40
carnosine	100 ppm	1.20

When thiamine, pyridoxine, folic acid, cyanocobalamin, aluminum sucrose octasulfate, lupeol and carnosine were used as active ingredients, although there was a difference in the treatment concentration for each active ingredient, the mRNA expression level of hyaluronic acid synthase was found to be at a significant level, It was confirmed that aluminum sucrose octasulfate showed a remarkably excellent effect.

Experimental Example 4. Efficacy evaluation for melanin production of melanocytes by active fraction

The effect of each active ingredient on the amount of melanin production in melanocytes of the skin was evaluated. As melanocytes, melanocyte B16 was used, and Dulbecco's modified containing penicillin (100 IU/ml), streptomycin (100 μg/ml) (GibcoBRL, USA) and 10% fetal bovine serum (FBS, GibcoBRL, USA). eagles medium (DMEM, GibcoBRL, USA) was added and cultured in an incubator (Forma Scientific, France) containing 5% carbon dioxide at 37°C. 1 x 10 ⁵ cells per well were dispensed in a 6-well culture plate and cultured for 24 hours. Herein, each active ingredient was added to DMEM without serum and treated for 72 hours.

The treatment concentration for each active ingredient is as shown in Table 4 below, and the melanin production amount of the control group that was not treated with anything was set at 100 and compared with relative values.

active ingredient	treatment concentration	Relative melanin production
Thiamine	10 ppm	88.2
pyridoxine	100 ppm	85.9
folic acid	100 ppm	88.5
Cyanocobalamin	10 ppm	84.3
Glycyretinic Acid	100 ppm	87.1
aluminum sucrose Octasulfate	1 ppm	80.5
lupeol	1 ppm	93.1
carnosine	100 ppm	65.9
	1,000 ppm	77.6

When thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol and carnosine were used as active ingredients, although there was a difference in the treatment concentration for each active ingredient, the reduction in melanin production was found to be significant.

Experimental Example 5. Efficacy evaluation for antibacterial peptide synthesis by active ingredient

The effect of each active ingredient on the synthesis of antibacterial peptides in skin cells was evaluated. HaCaT cells were used as skin cells, and Dulbecco's modified containing penicillin (100 IU/ml), streptomycin (100 μg/ml) (GibcoBRL, USA) and 10% fetal bovine serum (FBS, GibcoBRL, USA). eagles medium (DMEM, GibcoBRL, USA) was added and cultured in an incubator (Forma Scientific, France) containing 5% carbon dioxide at 37°C. 1 x 10 ⁶ cells were dispensed per well in a 24-well culture plate and cultured for 24 hours. Here, each active ingredient was added to DMEM without serum and treated for 24 hours. Then, after exposure at 42°C, a condition that inhibits the production of antibacterial peptides, for 15 minutes, 100 μl of the cell culture medium was removed and the amount of HBD2 (human beta defensin2) production, an antibacterial peptide, was measured by ELISA (Alpha Diagnostic. Intl., 100-240-BD2). was used for quantification. 100 μl of the culture medium was added to the HBD2 antibody-coated wells and then treated with HBD2 antibody capable of binding to HRP (horseradish peroxidase). After that, a substrate (TMB, Tetramethyl benzidine) reacting with HRP was added, and the color change as the substrate was decomposed was quantified by measuring the absorbance at 450 nm with an ELISA reader. During the experiment, a series dilution of HBD2 standard material (Alpha Diagnostic. Intl., 100-240-BD2) was performed and absorbance was measured at 450 nm in the same manner. After calculating the equation corresponding to the graph, the absorbance value of the sample was substituted for y to obtain the concentration of HBD2 corresponding to the x value. The HBD2 concentration value of the untreated control group was regarded as 100%, and the HBD2 concentration of each sample group was converted to a percentage and shown in Table 5.

active ingredient	treatment concentration	HBD2 production sensitivity (%)
Thiamine	1ppm	203.3
	10ppm	209.9
	100ppm	190.1
pyridoxine	1ppm	149.2
	10ppm	138.9
	100ppm	177.1
folic acid	0.1ppm	170.4
	1ppm	132.5
	10ppm	114.6
Cyanocobalamin	100ppm	122.5
Glycyretinic acid	0.1ppm	137.6
	1ppm	193.4
aluminum sucrose Octasulfate	100ppm	132.7
lupeol	100ppm	122.8
carnosine	100ppm	141.1

When thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol and carnosine were used as active ingredients, although there was a difference in the treatment concentration for each active ingredient, the increase rate of HBD2 production was significant. It was confirmed that glycyrrhetinic acid showed a remarkably excellent effect.

Experimental Example 6. Efficacy evaluation for collagen type 7 synthesis in skin cells by active ingredient

The effect of each active ingredient on collagen type 7 synthesis in skin cells was evaluated. HaCaT cells were used as skin cells, and Dulbecco's modified containing penicillin (100 IU/ml), streptomycin (100 μg/ml) (GibcoBRL, USA) and 10% fetal bovine serum (FBS, GibcoBRL, USA). eagles medium (DMEM, GibcoBRL, USA) was added and cultured in an incubator (Forma Scientific, France) containing 5% carbon dioxide at 37°C. 1 x 10 ⁶ cells were dispensed per well in a 24-well culture plate and cultured for 24 hours. Here, each active ingredient was added to DMEM without serum and treated for 24 hours. The amount of collagen 7 synthesis was quantified using ELISA (LSOBIO, Human COL7A1/Collagen VII ELISA kit, LS-F11164).

The treatment concentration for each active ingredient is as shown in Table 6 below, and the degree of increase or decrease is expressed as a percentage compared with the control group that is not treated with anything.

active ingredient	treatment concentration	Collagen 7 synthesis amount (%)
Thiamine	100 ppm	12.6
pyridoxine	10 ppm	8.1
folic acid	10 ppm	7.0
Glycyretinic Acid	1 ppm	12.0
carnosine	10 ppm	55.0

When thiamine, pyridoxine, folic acid, glycyrrhetinic acid, and carnosine were used as active ingredients, although there was a difference in treatment concentration for each active ingredient, the mRNA expression level of collagen 7 was found to be significant.

Experimental Example 7. Efficacy evaluation of carnosine on the expression of antioxidant proteins in skin cells

The effect of carnosine was evaluated by quantifying the expression level of antioxidant protein in skin cells. As skin cells, HaCaT cells were treated with Dulbecco's modified eagles medium (FBS, GibcoBRL, USA) containing penicillin (100 IU/ml), streptomycin (100μg/ml) (GibcoBRL, USA) and 10% fetal bovine serum (FBS, GibcoBRL, USA). DMEM, GibcoBRL, USA) was added, and culture was performed in an incubator (Forma Scientific, France) containing 5% carbon dioxide at 37°C. 1 x 10 ⁶ cells were dispensed per well in a 24-well culture plate and cultured for 24 hours. After dispensing in (basic culture medium and active ingredient condition information requested), UVB 50mJ/cm ² was treated (30W, 312nm, BIO-SUN, Vilber Lourmat Deutschland GmbH) to reduce SPRR1a (small proline rich protein 1a), Here, cells were treated with 20 ppm of carnosine in DMEM without serum for 24 hours. Thereafter, the degree of recovery of SPRR1a expression level was measured. Proteins were extracted from the cultured cells using a lysis buffer, and proteins were separated by size through SDS-PAGE, transferred to a membrane, and then the proteins were quantified using the SPRR1a antibody.

6 is a western blot result of the SPRR1a protein before UV treatment, after UV treatment as a control group, and after UV treatment at 20 ppm carnosine treatment. As a result of carnosine treatment, it was confirmed that the expression of SPRR1a was increased by 338% compared to the UV-treated control group that was not treated with anything.

It goes without saying that the present invention is not limited to the embodiments described above, and a combination of the embodiments or a combination of at least one of the embodiments and a known technology may be included as another embodiment.

Although the present invention has been described in detail through specific examples, this is for the purpose of describing the present invention in detail, and the present invention is not limited thereto. It will be clear that the transformation or improvement is possible.

All simple modifications and variations of the present invention fall within the scope of the present invention, and the specific scope of protection of the present invention will be made clear by the appended claims.

Claims (15)

1. Thiamine, pyridoxine, folic acid, cyanocobalamin, glycyrrhetinic acid, aluminum sucrose octasulfate, lupeol, carnosine and at least one selected from the group consisting of pharmaceutically acceptable salts thereof as an active ingredient A cosmetic composition, characterized in that
2. The method of claim 1,

   The cosmetic composition,

   A cosmetic composition, characterized in that it improves skin regeneration.
3. 3. The method of claim 2,

   The cosmetic composition,

   A cosmetic composition, characterized in that it increases the cell proliferation ability of the skin.
4. The method of claim 1,

   The cosmetic composition,

   A cosmetic composition, characterized in that it improves wrinkles or elasticity of the skin.
5. 5. The method of claim 4,

   The cosmetic composition,

   A cosmetic composition, characterized in that it increases the collagen synthesis ability of skin cells.
6. The method of claim 1,

   The cosmetic composition,

   A cosmetic composition for moisturizing the skin.
7. 7. The method of claim 6,

   The cosmetic composition,

   A cosmetic composition, characterized in that it increases the expression rate of hyaluronic acid synthase in skin cells.
8. The method of claim 1,

   The cosmetic composition,

   A cosmetic composition for skin whitening or brightening.
9. 9. The method of claim 8,

   The cosmetic composition,

   A cosmetic composition, characterized in that it inhibits the synthesis of melanin in melanocytes.
10. The method of claim 1,

    The cosmetic composition,

    A cosmetic composition for improving the skin barrier.
11. 11. The method of claim 10,

    The cosmetic composition,

    A cosmetic composition, characterized in that it increases the production of antibacterial peptides in skin cells.
12. 11. The method of claim 10,

    The cosmetic composition,

    A cosmetic composition characterized in that it increases the amount of collagen 7 synthesis in skin cells.
13. 11. The method of claim 10,

    The cosmetic composition,

    A cosmetic composition, characterized in that it increases the expression level of antioxidant protein in skin cells.
14. 14. The method according to any one of claims 1 to 13,

    The composition includes lotion, facial lotion, body lotion, nourishing cream, moisture cream, eye cream, essence, cosmetic ointment, spray, gel, pack, sunscreen, makeup base, foundation, powder, cleansing cream, cleansing lotion, cleansing oil, A cosmetic composition, characterized in that it is a cleansing foam, soap or body wash formulation.
15. 14. The method according to any one of claims 1 to 13,

    The skin is the scalp,

    The composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, hair aerosol, Hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair A cosmetic composition, characterized in that it is in the form of a mousse or hairspray.

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