KR20170128098A - Skin external agent for skin whitening comprising an extract of fermented wheat germ - Google Patents

Abstract

The present invention relates to an external composition for skin whitening containing a fermented wheat germ or an extract thereof as an effective component, or relates to an external composition for preventing, improving or treating pigmented skin. The fermented wheat germ is obtained by grafting yeast onto wheat germs or culture medium including the germs and then cultivating the wheat germs. The composition of the present invention is capable of having proper stability without toxicity as well as suppressing the activation of tyrosinase, impeding melanin synthesis, and improving pigmented skin, thereby being used as an external composition for skin whitening or preventing, improving, and treating pigmented skin.

Description

[0001] The present invention relates to a skin external agent for skin whitening,

The present invention relates to a composition for external application for skin whitening comprising an extract of a fermented product of wheat germ as an active ingredient.

Skin exposed to ultraviolet rays protects the body from ultraviolet rays by synthesizing melanin pigment in melanocytes. The melanin pigment, which is temporarily synthesized by ultraviolet stimulation, migrates to surrounding keratinocytes and accumulates. Increased synthesis and accumulation of melanin pigment causes pigmentation on the skin, resulting in black coloration. In order to inhibit such pigmentation, a method of inhibiting the synthesis of melanin producing enzyme tyrosinase or inhibiting its activity to reduce the production of melanin has been proposed.

Known whitening ingredients include albutin, kojic acid, azelaic acid, aloesin, 4-butyl resorcinol, resveratrol, ceramide, sphingosine-1-phosphate, sphingolipid, (L-ascorbic acid) and derivatives thereof, and green tea leaf-derived extracts (Korea Patent Laid-Open Publication No. 2015-0025540), hydroquinone (Korean Patent Publication No. 2016-0014271) .

However, when they are applied to the skin, there is a problem that they are limited in the amount of use due to problems of skin safety such as irritation and redness, or that the effect is insignificant. In particular, hydroquinone also suffers from safety problems due to the addition of compounds to counteract its stimuli.

Therefore, there is a demand for the development of a whitening ingredient which is safe to the living body and is superior in ability to inhibit melanin synthesis than existing substances.

Under these circumstances, the present inventors have made intensive efforts to develop an excellent composition for external whitening skin whitening, and as a result, found that the extract of the wheat germ fermented product is excellent in safety and excellent in the effect of inhibiting melanin synthesis, .

The object of the present invention is to provide a skin whitening cosmetic composition containing a wheat germ fermented product or an extract thereof.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating dermatological pigmentary diseases containing wheat germ fermented product or extract thereof.

It is still another object of the present invention to provide a method for preventing or treating dermatological pigmentary diseases, comprising the step of administering a fermented product of wheat germ or an extract thereof or a pharmaceutical composition of the present invention to a subject in need thereof.

It is another object of the present invention to provide a quasi-drug composition for skin whitening comprising a fermented product of wheat germ or an extract thereof.

It is yet another object of the present invention to provide a method for whitening skin, comprising the step of administering the wheat germ fermented product or extract thereof, or the composition of the present invention to a subject in need thereof.

As one aspect for attaining the object of the present invention, the present invention provides a cosmetic composition for skin whitening comprising a wheat germ fermented product or an extract thereof.

As used herein, the term "wheat germ" means wheat germ. This is a nutrient-rich embryo of wheat kernel that is removed from the flour milling process, accounting for about 2 to 3% of the total wheat grain. Wheat germs are known to be rich in vitamin E (tocopherol), known to be antioxidant vitamins so far, and recent studies have shown that natural substances extracted from fermented wheat germs have the effect of restoring the damaged immune function of the human body. However, skin whitening effects of wheat embryos are not known.

The wheat embryo herein may include, but is not limited to, the dried, ground, concentrated, and mixtures thereof of the wheat embryo.

As used herein, the term "fermentation" means any activity or process involving enzymatic or metabolic degradation of an organic material with a microorganism.

As used herein, the term "wheat germ fermentation product" refers to the result of enzymatic or metabolic degradation of wheat germ using microorganisms.

The fermented product of the wheat germ in the present invention may be obtained by inoculating a microorganism into a wheat embryo or a culture medium containing the same and culturing the same. Specifically, the microorganism may be at least one selected from the group consisting of yeast, lactic acid bacteria, bacteria, and fungi.

The yeast may be, for example, Saccharomyces cerevisiae , S. ellipsoideus , S. coreanus , Saccharomyces cylindusensis (" Saccharomyces cerevisiae " S. carlsbergensis), Saccharomyces access to my Paz Astoria Augustine (S. pastorianus), Saccharomyces access to my lactis (S. lactis), Saccharomyces access to my ruksi (S. rouxii), to access Shijo Saccharomyces pombe Mai (Schizosaccharomyces pombe ), Zygosaccharomyces major , Hansenula anomala , Brettanomyces bruxellensis , B. custersianus , Dekkera , cyano do (Dekkera anomala), Streptomyces up balk our jeneseu (Streptomyces olivochromogenes), or Streptomyces draw three-house (S. griseus), but the purpose can be skin whitening effect of the present application A no longer be limited, which can be represented. For example, the yeast may be Saccharomyces cerevisiae.

The lactic acid bacteria may be, for example, Bifidobacterium sp.), Lactobacillus genus (Lactobacillus sp.), Lactococcus lactis in (Lactococcus sp.), Peddie Oh caucus in (Pediococcus sp.), Streptococcus genus (Streptococcus. sp), or the current Kono Stock in (Leuconostoc sp.), but are not limited to those which can exhibit skin whitening effects for the purposes of this application. For example, the lactic acid bacteria is Bifidobacterium bipyridinium bushes (B. bifidum), Bifidobacterium breather probe (B. breve), Bifidobacterium ronggeom (B. longum), Bifidobacterium Annie Marlies (B. animalis), Bifidobacterium lactis (B. lactis), Lactobacillus ash's also a filler (L. acidophilus), Casey Lactobacillus (L. casei), Lactobacillus joined Lee (L. gasseri), Lactobacillus del Brewer L. delbrueckii spp., L. bulgaricus , L. helveticus , L. fermentum , L. paracasei , Lactobacillus spp., Lactobacillus sp. L. plantarum , L. reuteri , L. rhamnosus , L. salivarius , L. lactis , and the like), Lactobacillus spp ., Lactobacillus sp. , Peddie Oh Celebi jiae Caucus (P. cerevisiae), Peddie Oh Caucus kids CD Rock City (P. acidilactici), Streptococcus lactis (S. lactis), Streptococcus thermostat Phillies (S. thermophiles), Streptococcus Crescent Morris (S. cremoris), or the current mail center Stock Pocono Roy Rhodes (L. mesenteroides) Lt; / RTI > Lactic acid bacteria may be used herein in the same sense as lactic acid bacteria.

The fungus used in the fermentation may include, but is not limited to, mushrooms. Examples of the mushrooms include shiitake mushroom, oyster mushroom, mushroom mushroom, mushroom mushroom, mushroom mushroom, mushroom mushroom, Ginger mushroom, or mushroom, but is not limited thereto, as long as it can exhibit a skin whitening effect for the purpose of the present invention.

The amount of inoculum, culture temperature, culture humidity, and incubation time of the microorganism used to produce the wheat germ fermented product of the present invention can be appropriately selected by those skilled in the art in consideration of the kind of microorganism used for fermentation. As a non-limiting example, the cultivation of the present invention can be carried out at a temperature of 20 ° C to 40 ° C for 12 to 60 hours. Specifically, the cultivation of the present invention is carried out at 25 ° C to 35 ° C, 28 ° C to 32 ° C or 30 ° C and / or 12 hours to 50 hours, 20 hours to 50 hours, 30 hours to 50 hours, Hour to 42 hours or 40 hours.

As used herein, the term "extract of wheat germ fermented product" refers to a substance from which microorganisms have been removed from a wheat germ fermentation product.

The removal of the present invention may include any method for removing microorganisms (for example, yeast cells) contained in microbial fermentations known in the art. Specifically, the extract of the wheat germ fermented product of the present invention may be a supernatant obtained by subjecting the wheat germ fermented product of the present invention to centrifugation. More specifically, the centrifugation may be carried out at a rotational speed of 6,000 rpm to 10,000 rpm, 7000 rpm to 9000 rpm, 7500 rpm to 8500 rpm, 7800 rpm to 8200 rpm or 8000 rpm and / 5 minutes to 40 minutes, 5 minutes to 30 minutes, 10 minutes to 120 minutes, 10 minutes to 90 minutes, 10 minutes to 60 minutes, 10 minutes to 40 minutes, 15 to 30 minutes, 15 to 120 minutes, 15 to 90 minutes, 15 to 60 minutes, 15 to 40 minutes, 15 to 30 minutes, 15 to 25 minutes, or 20 minutes.

The extract of the wheat germ fermented product of the present application includes the extract of the wheat germ fermented product of the present invention and an extract of all the formulations which can be formed using an extract such as a dilute solution, a concentrate, a dried product, a controlled preparation, a purified product or a mixture thereof. Specifically, the extract of the wheat germ fermented product of the present invention may be a dried product, more specifically, a lyophilized product.

In addition, the extract of the wheat germ fermented product of the present invention can be obtained from the wheat germ fermentation by any method as long as it has a skin whitening effect or prevention, improvement or therapeutic effect of dermatological pigmentary diseases, A method such as a cold extraction method in which water is immersed in a solvent and extraction is performed at room temperature of 10 to 25 ° C, a heating extraction method in which heating is carried out at 40 to 100 ° C, an ultrasonic extraction method in which ultrasonic waves are extracted, and a reflux extraction method using a reflux condenser . These methods may be performed alone or in combination of two or more methods.

The kind of the extraction solvent used in the extraction of the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent of the present invention include water, a C1-4 alcohol, hexane, ethyl acetate, chloroform, dichloromethane and mixtures thereof And the extraction solvent of the present invention may be hydrothermal.

The extract of the present invention may comprise fractions thereof. The term "fraction " as used herein refers to the result obtained by performing a fractionation to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fractions of the present invention is not particularly limited and may be carried out according to a method commonly used in the art. A solvent fractionation method performed by treating various solvents, an ultrafiltration fractionation method performed by passing through an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity ), A combination thereof, and the like.

The kind of the fraction solvent used to obtain the fraction of the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvents of the present application include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of one or more.

The wheat germ fermented product or the extract thereof of the present invention may be used in an amount of 0.001 to 10% by weight based on the total weight of the composition of the present invention, specifically 0.01 to 7% by weight, 0.01 to 5% by weight, 0.01 0.05 to 10% by weight, 0.05 to 7% by weight, 0.05 to 5% by weight, 0.05 to 1% by weight, and 0.01 to 3% 0.1 wt.% To 7 wt.%, 0.1 wt.% To 5 wt.%, 0.1 wt.% To 3 wt.% %, 0.1% to 2%, 0.1% to 1%, 0.5% to 10%, 0.5% to 7%, 0.5% to 5%, 0.5% to 3% From 0.5% to 2%, from 0.5% to 1%, from 0.8% to 10%, from 0.8% to 7%, from 0.8% to 5%, from 0.8% to 3% %, 0.8 wt% to 2 wt%, 0.8 wt% may be included as to 1% or 1% by weight.

The wheat germ fermented product or extract thereof of the present invention may be contained in the composition of the present invention at 0.1 μg / ml to 100 μg / ml. Specifically, the wheat germ fermented product of the present invention or an extract thereof may be added to the composition of the present invention at a concentration of 0.1 μg / ml to 70 μg / ml, 0.1 μg / ml to 50 μg / ml, 0.5 μg / ml to 70 μg / ml, 1 μg / ml to 50 μg / ml, 5 μg / ml, and 1 μg / ml to 50 μg / ml, 0.8 μg / ml to 70 μg / ml, 0.8 μg / ml to 50 μg / ml, Ml to 50 μg / ml, 8 μg / ml to 70 μg / ml, 8 μg / ml to 50 μg / ml, 10 μg / ml to 70 μg / ml, or 10 μg / ml To 50 μg / ml.

According to one embodiment herein, the compositions herein may inhibit tyrosinase activity and / or inhibit melanin synthesis.

The cosmetic composition of the present invention may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, And may be formulated and provided in a selected form.

The cosmetic composition of the present application may further comprise a cosmetically acceptable carrier.

The kind of the cosmetically acceptable carrier of the present invention is not particularly limited so long as it does not impair the activity and properties of the cosmetic composition of the present invention, and any cosmetically acceptable carrier conventionally used in the art can be used. Non-limiting examples of the cosmetically acceptable carrier include saline, sterilized water, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and the like. These may be used alone or in combination of two or more. The cosmetically acceptable carriers herein may comprise non-naturally occuring carriers.

The cosmetically acceptable carriers herein vary according to the formulation of the cosmetic composition.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, it is preferable to use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used as a carrier component. But are not limited to, propellants such as hydrocarbons, propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Propylene glycol, 1,3-butyl glycol oil and the like can be used. Particularly, it is possible to use an oil such as cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan Fatty acid esters can be used, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto.

When the formulation of the cosmetic composition of the present invention is a soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, But is not limited thereto.

In the case where the formulation of the cosmetic composition of the present invention is a pack, a fill-off pack containing polyvinyl alcohol or the like, a wash-off pack containing a pigment such as kaolin, talc, zinc oxide, , Or a mask sheet pack, and is not particularly limited thereto.

The cosmetic composition of the present invention may further comprise components included in conventional skin external agent components such as water, a surfactant, a moisturizer, a lower alcohol, a chelating agent, a bactericide, an antioxidant, an antiseptic, a pigment and a fragrance.

As another aspect for achieving the object of the present invention, the present invention provides a pharmaceutical composition for preventing or treating dermatological pigmentary diseases containing wheat germ fermented product or extract thereof.

In the pharmaceutical composition of the present application, the wheat germ, the wheat germ fermented product and the extract thereof are all applied as described above. The term "dermatological pigmentary disease" as used herein means a disease having a symptom of excessive deposition of pigment on the skin. Specifically, the dermatological pigmentation disease may be stain, freckles, dullness or melanoma.

As used herein, the term "prevention" refers to any action that inhibits or delays the occurrence of a symptom of dermatological pigmentary disease, such as spots, freckles, dullness or melanoma.

As used herein, the term "treatment" refers to any action whereby the pharmaceutical composition of the present invention alleviates or alleviates the symptoms of dermatological pigmentary diseases such as spots, freckles, dullness, or melanoma.

In the present application, the pharmaceutical composition may further comprise a pharmaceutically acceptable carrier.

As used herein, the term "pharmaceutically acceptable carrier" refers to a carrier or diluent that does not irritate the organism and does not interfere with the preventive or therapeutic activity and properties of the dermatological pigment disease symptom of the composition. Examples of the pharmaceutical carrier which is acceptable for the composition to be formulated into a liquid solution include sterilization and sterilization which are suitable for a living body and include saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol And one or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added.

In addition, the pharmaceutically acceptable carriers herein may comprise non-natural carriers.

The pharmaceutical compositions herein may be administered in single or multiple doses in a pharmaceutically effective amount.

As used herein, the term "pharmaceutically effective amount" means an amount sufficient to prevent or treat a disease at a reasonable benefit / risk ratio applicable to medical prophylaxis or therapy, and the effective dose level will depend on the severity of the disease, , The body weight of the patient, the health, the sex, the sensitivity of the patient to the drug, the time of administration of the composition used, the route of administration and the rate of excretion, the duration of the treatment, factors including drugs used in combination with or co- May be determined by factors well known in the medical arts.

In another aspect for achieving the object of the present invention, the present invention provides a method for preventing or treating dermatological pigmentary diseases, comprising the step of administering a wheat germ fermented product, an extract thereof, or a pharmaceutical composition of the present invention to a subject in need thereof ≪ / RTI >

In the method for preventing or treating dermatological pigmentary diseases of the present invention, the wheat germ, the wheat germ fermented product, the extract of the wheat germ fermented product, and the dermatological pigmentation disease, prevention and treatment are as described above.

The term "administering " as used herein means introducing a given substance into a subject by any suitable method, and the composition of the present invention may be administered via any conventional route by which it can reach a target in vivo. The administration route of the composition of the present invention is not particularly limited, but may be orally or parenterally. Specifically, it can be administered parenterally, and more specifically, it can be applied to the skin (i.e., transdermal administration). Specifically, the administration of the present invention can be carried out once to four times a day, twice to three times, or twice. In addition, administration of the present invention can be carried out for more than 4 weeks, more than 8 weeks, 4 to 12 weeks or 8 to 12 weeks.

According to another aspect of the present invention, there is provided a skin whitening quasi-product composition containing a wheat germ fermented product or an extract thereof.

The extracts of wheat germ, wheat germ fermented product, and wheat germ fermented product in the quasi-drug composition of the present invention are as described above.

As used herein, the term " quasi-drug "refers to products which are used for diagnosis, treatment, improvement, alleviation, treatment or prevention of diseases of human or animal, Quasi-drugs are products that are used for the purpose of treating or preventing diseases of humans or animals, products that are mild or have no direct action on the human body.

The quasi-drug composition of the present invention can be manufactured in a form selected from the group consisting of body cleanser, foam, soap, mask, ointment, cream, lotion, essence and spray, but is not limited thereto.

When the wheat germ fermented product of the present invention or an extract thereof is used as a quasi-drug additive, the wheat germ fermented product or its extract of the present invention can be used as it is or can be used in combination with other quasi-drugs or quasi-drug components and can be suitably used according to a conventional method .

In another aspect for achieving the object of the present invention, the present invention provides a method for whitening skin, comprising the step of administering a wheat germ fermented product, an extract thereof, or a composition of the present invention to a subject in need thereof .

In the method of whitening the skin of the present invention, the wheat germ, the wheat germ fermented product, the extract of the wheat germ fermented product, the composition and administration of the present invention are as described above.

The wheat germ fermented product, its extract, or the composition of the present invention may be administered to an individual in need thereof in an effective amount.

 The level of effective dose can be determined by a person skilled in the art on the basis of common sense to the extent that the desired skin whitening effect is exhibited.

The composition of the present invention has no toxicity and is not only stable, but also inhibits tyrosinase activity, inhibits melanin synthesis, and improves pigmentation to provide a skin whitening external preparation, a dermatological pigmentation prevention, It can be used as a therapeutic external preparation.

FIG. 1 is a graph showing the results of performing a cytotoxicity test on an extract of wheat germ fermented product (denoted as CJ) on Melan-a cells according to an embodiment of the present invention.
FIG. 2 is a graph showing inhibition of tyrosinase activity of the extract of wheat germ fermented product (CJ) according to one embodiment of the present invention.
FIG. 3 is a graph showing inhibition of melanin synthesis of the extract (CJ) of the wheat germ fermented product according to one embodiment of the present invention.
FIG. 4 is a graph comparing changes in visual evaluation of skin color according to the use of the product of the wheat germ fermented product extract-containing product (CJD cream) and the negative control product (control cream) according to one embodiment of the present invention.
FIG. 5 is a graph comparing the increase / decrease rate of the skin melanin index (MI) according to the use time of the product of the extract of wheat germ fermented product (CJD cream) and the negative control product (control cream) according to one embodiment of the present invention.

Hereinafter, the present invention will be described in more detail in the following examples. However, these examples are provided only for the understanding of the present application, and the present invention is not limited thereto.

Manufacturing example  One: Of fermented wheat germ  Extract preparation

50 g of wheat embryo (CJ Cheiljedang) and 500 g of water were added to the flask, followed by thorough mixing and sterilization at 121 占 폚 for 15 minutes. Then, 5% (2.5 g) of dry yeast (Baker's yeast (Angelica japonica), Angel Yeast) was inoculated on the wheat germ, and fermented at 30 DEG C for 40 hours and then incubated at 8000 rpm for 20 minutes The supernatant was taken by centrifugation. The supernatant was lyophilized and recovered to prepare an extract of the wheat germ fermented product.

In this Example, the extract of the wheat germ fermented product was named 'CJ'.

Experimental Example 1 : Cell viability assay (cell viability assay)

The cytotoxicity of the wheat germ fermented extract (CJ) of Preparation Example 1 was measured in melan-a cells.

Specifically, melan-a cells were plated in 96-well plates at 5 × 10 ⁴ cells / well. Then, 10% fetal bovine serum (FBS), 50 U / ml penicillin, 50 μg / ml streptomycin, 200 nM povol 12 The cells were cultured in a medium supplemented with phobol 12-myristate 13-acetate (PMA) at 37 ° C and 10% CO ₂ for 24 hours. After the incubation, the starvation state was maintained for 12 hours, and the RPMI 1640 medium (control), from which the extract of the wheat germ fermentation product of Preparation Example 1 and the additive (supplement) were excluded, Respectively. To measure the cell viability, WST-1 (water-soluble tetrazolium salt-1, Roche) was diluted 1/10 in RPMI 1640 medium without additives and treated with 100 μL per well. After reacting for a period of time, the absorbance was measured at 450 nm. Thereafter, the relative cell viability of the melan-a cells treated with the extract (CJ) of the wheat germ fermented product of Preparation Example 1 (experimental group) was determined for RPMI 1640 treated group (control group) except for the additive.

As a result, it was confirmed that no cytotoxicity was observed when the extract of the wheat germ fermented product was treated at 100 μg / ml or less (FIG. 1). Therefore, in the following Examples and Experimental Examples, the concentration of the extract of the wheat germ fermentation product was set to 50 μg / ml or less.

Experimental Example  2: Evaluation of whitening efficacy

2-1: Tyrosinase ( tyrosinase ) Active inhibition confirmation

20 μl of a 0.1 M phosphate buffer solution in a test tube, 20 μl of HBSS (Hanks' Balanced Salt Solution, Sigma-Aldrich) dilution of the extract of the wheat germ fermentation product of Preparation Example 1 and 20 μl of a mushroom tyrosinase solution (1500 U / ml) And 40 μl of a 1.5 mM tyrosine solution was added to prepare a sample solution. The sample solution was reacted at 37 ° C. for 10 minutes to 15 minutes, and absorbance was measured at 490 nm using an ELISA reader. As the blank, the 0.1 M phosphate buffer (pH 6.5) was added to the sample solution in place of the HBSS dilution of the extract of the wheat germ fermented product. Then, the activity inhibition rate was calculated as shown in the following formula 1

[Formula 1]

a: Absorbance after reaction of blank sample

b: Absorbance after reaction of the sample solution

a ', b': absorbance measured by replacing with buffer instead of tyrosine

As a result, the extract of wheat germ fermented product (CJ) showed a strong enzyme similar to that of the positive control [arbutin, 1 mM, '+'] shown at the concentration of 10 μg / ml and 50 μg / ml (Fig. 2).

2-2: Confirmation of inhibition of melanin synthesis

Melan-a cells were added to a 24-well plate so as to be 3x10 ⁴ cells / well. Then, the cells were cultured in RPMI 1640 medium for 24 hours in a cell incubator so that the cells adhered well to the plate. The extracts (CJ) of the embryo fermented product were dissolved in RPMI 1640 medium and added twice at three times every 6 days for each concentration (1 ㎍ / ml, 10 ㎍ / ml, 50 ㎍ / ml) for 6 days. Then, the cells were dissolved in cell lysis buffer (Bio-rad), and the supernatant was separated by centrifugation at 17,000 rpm for 30 minutes to quantitate the protein (BCA method), and 1N NaOH was added thereto at 405 nm Absorbance was measured and the amount of melanin relative to the amount of protein measured was calculated.

As a result, the melanin synthesis inhibitory activity of the extract of the wheat germ fermented product was particularly excellent at a concentration of 10 ㎍ / ml or more, and this activity was confirmed by treating the arbutin (1 mM) with the treatment group (positive control, ) (Fig. 3).

Example  One: Wheat embryo Fermented  Extract-containing Cosmetic  Whitening efficacy evaluation

1-1. Make-up cream

A cosmetic cream containing an extract of the wheat germ fermented product of Production Example 1 as an active ingredient was prepared according to a conventional method as in the composition (% by weight) shown in Table 1 below.

1-2. Whitening cream Clinical assessment of efficacy

1-2-1. Outline of experiment

The skin whitening effect of the makeup cream (hereinafter referred to as "CJD cream") prepared in Example 1-1 was evaluated in the negative control group (cream containing the same amount of purified water in place of the extract of the wheat germ fermentation product in the composition of Table 1, " control cream "), respectively. The subjects were 22 Korean women aged between 30 and 55 with pigmented skin and pigmentation.

After randomization (block randomization), the control cream and CJD cream were applied for 12 weeks. After 12 weeks of use, (Skin melanin index) and safety evaluation were carried out to evaluate the effectiveness of the cream containing the extract of wheat germ fermented product for whitening of human skin. Specific sample information and methods of use are summarized in Table 2 below.

Clinical evaluation subjects were asked to wash their skin at each evaluation point, and after 20 minutes of skin stabilization in a space where air movement and direct sunlight were not observed under constant temperature and humidity conditions (22 ± 2 ° C, 50 ± 5%), . The measurement and evaluation procedures are summarized in Table 3 below.

1-2-2. Visual evaluation of skin color

Visual evaluation of skin color (including pigmentation) was carried out by two testers at 0 to 9 levels according to the visual evaluation criteria shown in Table 4.

As a result, as shown in Table 5, when compared with before use of the product, at the 8th week after using the product, the skin color visual evaluation score of the CJD cream use group was statistically significantly decreased, and the effect of brightening (whitening) ≪ 0.05).

As a result of the comparison between the groups, as shown in Table 6 and FIG. 4, the CJD cream had a statistically significant decrease in the skin visual evaluation score as compared with the control cream group at 8 weeks, and the whitening effect of CJD cream (P < 0.05).

1-2-3. Melanin index measurement

The skin melanin index (up to 8 weeks) and skin color were measured on the right and left cheeks of the subjects before use (0 weeks), 4 weeks, 8 weeks, and 12 weeks of use.

Mexameter® MPA580 (C + K, Germany) is a device that measures the amount of melanin and hemoglobin, which are the main factors that determine skin color, using the principle of absorption. The melanin index is measured as the absorption rate in two different wavelength regions. The erythema index is measured as the absorption rate of the absorption band of hemoglobin and the skin absorption rate in the wavelength range excluding the interference of other pigments such as bilirubin. The skin absorption rate of each wavelength region is digitized and indicated by Melanin Index (E.I.) and Erythema Index (E.I.). In this study, the test site (cheek, pigmentation site) was repeatedly measured three times at each evaluation point and the average value of Melinin index was analyzed to obtain accurate measurement values.

The skin melanin index (M> I) of the CJD cream group was significantly reduced (improved) in both the cheek area and the pigmentation area at 4 weeks and 8 weeks after use of the product (FIG. 5) . On the other hand, the melanin index (MI) of the control cream group was significantly reduced (improved) only in the cheek area after 8 weeks of use. Table 7 summarizes the change of melanin index by time.

On the other hand, the skin melanin index (MI) of the CJD creams decreased significantly (at 4 weeks after using the product) compared to the control cream group. Table 8 shows comparison data between groups of changes in skin melanin index at different time points.

6-2-4. Safety evaluation

After 4 weeks, 8 weeks, and 12 weeks after the use of the test product (CJD cream and control cream), the skin condition of the subject of clinical evaluation was observed and the skin condition for subjective and objective stimulation was confirmed through question and answer with the subject And recorded and evaluated. As a result, no skin adverse events were observed in all subjects undergoing clinical evaluation (Table 9).

From the foregoing, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described examples are illustrative in all aspects and not restrictive. The scope of the present invention should be construed to cover all modifications and variations derived from the meaning and scope of the appended claims rather than the foregoing detailed description and equivalents thereof.

Claims (10)

A skin whitening cosmetic composition containing wheat germ fermented product or extract thereof.
The method according to claim 1,
Wherein the wheat germ fermented product is obtained by inoculating yeast into a wheat embryo or a culture medium containing the same and culturing the same.
3. The method of claim 2,
Wherein the yeast is Saccharomyces cerevisiae, wherein the yeast is Saccharomyces cerevisiae.
The method according to claim 1,
Wherein said culturing is carried out at a temperature of 20 to 40 DEG C for 12 to 60 hours.
The method according to claim 1,
Wherein the wheat germ fermented product or the extract thereof contains 0.001 wt% to 10 wt% of the total cosmetic composition.
The method according to claim 1,
Wherein the composition inhibits tyrosinase activity. &Lt; RTI ID = 0.0 &gt; 18. &lt; / RTI &gt;
The method according to claim 1,
Wherein the composition inhibits melanin synthesis.
A pharmaceutical composition for preventing or treating dermatological pigmentary diseases containing wheat germ fermented product or extract thereof.
9. The method of claim 8,
Wherein the dermatological pigmentary disease is spots, freckles, spots or melanomas.
A composition for skin whitening comprising a wheat germ fermented product or an extract thereof.

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