KR20170100076A - Cosmetic composition comprising aged chrysanthemum - Google Patents
Cosmetic composition comprising aged chrysanthemum Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The present invention relates to a cosmetic composition containing a chrysanthemum matured product, wherein the composition obtained by the present invention exhibits a higher cell activity and an inflammation-relieving effect than a chrysanthemum extract using a conventional solvent, and the cosmetic composition containing the same also exhibits excellent anti- Anti-inflammatory and soothing effects.
Description
The present invention relates to a cosmetic composition containing chrysanthemums.
The extent to which aging of cells affects the aging of individuals (humans, animals) is not yet known. It is well known that the telomere at the end of the chromosome is shortened each time it breaks down and the cell dies when it reaches its limit. Recently, there has been research that collagen, which is the main component of cell epilepsy, is reduced to 65% of the 20s when it is in their 80s and is involved in aging. There are also studies that abnormal protein accumulation due to intracellular ER stress is involved in aging.
In skin aging, the three major components of connective tissue, collagen and proteoglycan, are catabolized by intermolecular cross-linking or branching, resulting in changes in cell-extracellular matrix interactions. In addition, the decrease of the polar cells and the turnover of the epidermal cells (turn over, usually 28 days) are reduced from 70 to 40% of their 30s, causing skin dullness. And collagen, ceramide reduction is the main cause of skin wrinkles due to aging. It has been reported that the administration of estrogen improves the moisturization by increasing the synthesis of hyaluronic acid as well as the collagen increase of the skin (EL-Domyati M et al .: Exp Dermatol 11, 2002)
Oxidative stress is known to be an important cause of aging. Cellular stressors include reactive oxygen species called reactive oxygen species (ROS), such as peroxides, hydroxy radicals, and hydrogen peroxide. RNS (reactive nitrogen species) such as nitric oxide and peroxynitrite are known, and lipid peroxides are also known as oxidative stress inducers. These oxides are known to bind to guanine, a DNA base, to induce mutagenesis, leading to skin aging and cancer. It has been reported that it participates in the arachidonic acid cascade and causes vasoconstriction and platelet aggregation to develop as a risk factor for arteriosclerosis or Alzheimer's disease. Vitamin C and B12 are known to be water soluble and lycopene and beta-carotene are known to be lipid soluble.
Chrysanthemum is a perennial herb belonging to the Compositae, mainly edible or medicinal. It is also called as "Ganja" and "Nakyanghwa". Chrysanthemums can be used in a variety of ways, including chrysanthemums, chrysanthemums, and tea. The pharmacological action of chrysanthemum has the functions of promoting blood circulation, warming, and pain. It can be used for fever, fever, headache, dizziness, earache, eye disease, tumor pain, fever, detoxification, analgesia and inflammation.
The effect of chrysanthemum on the primordial gangbok is' good for blood, take a long time and lighten the body, and do not grow old. It comforts the stomach, helps the five, and regulates the limbs. It is also effective for colds, headaches and dizziness. " In addition, references to the efficacy of chrysanthemum tea are also mentioned in the literature such as the Enhanced Forest Economy, Chungdang Shoten, and Divine Materia Medica. Chrysanthemum tea has these effects because it contains vitamin A, vitamin B1, choline, stachydrin, and adenine which are good for restoring eye and liver function among the components of chrysanthemum. Especially, chrysanthemum flowers contain trimethylcyclohexane, carbosilicic acid and acacetin, leaves and stems of adenine, and cholinestatidrine.
Although the usefulness of the above-mentioned chrysanthemum is found, the chrysanthemum has many problems to be used as it is, so it is a level of extracting useful components of chrysanthemum flower and chrysanthemum using the chrysanthemum flower as an additive. Korean Patent Publication No. 0278203 discloses a stress-relieved beverage produced by mixing ethanolic extracts of chrysanthemums with 12 kinds of food ingredients such as soybean embryo and bamboo shoots. However, this is merely the use of ethanol extract of chrysanthemum, and when chrysanthemum itself is used as it is, most of it remains in a state of concern with hydrothermal fluids, so that the functional materials contained in chrysanthemum can not be utilized sufficiently.
In order to solve the above-mentioned problems, the present invention provides a method for producing a chrysanthemum comprising the steps of mixing a small amount of saccharides and minerals in a sterilized environment to activate the enzyme, Respectively.
The present invention relates to a method for producing chrysanthemum chrysanthemums comprising mixing saccharide-sterilized saccharides and minerals with chrysanthemums and maintaining the sterilization conditions.
In the present invention, the mixture of saccharides and minerals with respect to the total weight of the aged material is not limited, but may be 1 to 10% by weight.
In the present invention, the saccharides and minerals are not limited, but sugar and minerals may be mixed in a weight ratio of 1 to 9: 1.
In the present invention, the saccharide may be, but not limited to, one or more selected from the group consisting of glucose, lactose, fructose, sugar, trehalose, xylitol, mannitol, sorbitol, erythritol and galacto-oligosaccharide.
In the present invention, the minerals may be any one or more selected from the group consisting of nephrite, talc, muscovite, sericite, kaolin, seamstone and organic germanium.
The present invention also relates to a cosmetic composition for preventing skin aging, improving wrinkles and moisturizing the skin, comprising the chrysanthemum matured product as an active ingredient.
The cosmetic composition of the present invention may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, And may be any one selected from the group consisting of.
The composition obtained by the present invention shows higher cell activity and inflammation-reducing effect than the conventional extracts of chrysanthemum using a conventional solvent, and it has been confirmed that the cosmetic composition containing the same exhibits excellent anti-aging, anti-inflammation and soothing effects.
The present invention relates to a method for producing chrysanthemum chrysanthemums comprising mixing saccharide-sterilized saccharides and minerals with chrysanthemums and maintaining the sterilization conditions.
The aging may be carried out under ordinary conditions in a region having a common temperature and pressure, but is not particularly limited, and is preferably 30 to 90 days at 15 to 30 DEG C, more preferably 30 to 50 days at 20 to 25 DEG C The antioxidative effect of the aged material is improved, the collagen production ability is increased, and the cytotoxicity is decreased.
In the present invention, the mixture of saccharides and minerals with respect to the total weight of the aged material is not limited, but may be 1 to 10% by weight.
In the present invention, the saccharides and minerals are not limited, but sugar and minerals may be mixed in a weight ratio of 1 to 9: 1.
According to one embodiment of the present invention, the expression of the chrysanthemum itself can be most effectively manifested within the above range, the cytotoxicity can be substantially eliminated, the inflammation can be smoothly improved, the skin lubrication can be improved, And the skin moisturizing effect is improved. Thus, the effect of improving skin aging can be remarkably improved.
In the present invention, the saccharide may be, but not limited to, one or more selected from the group consisting of glucose, lactose, fructose, sugar, trehalose, xylitol, mannitol, sorbitol, erythritol and galacto-oligosaccharide.
Glucose is widely contained in fruits, honey and vegetables in a small amount, and most of the commercially available crystalline glucose is prepared by hydrolyzing corn starch. Lactose is contained in the milk of all animals. Galactose and glucose are combined to form lactose. Fructose is widely distributed in plants along with glucose. Powdered honey is made by pulverizing honey. Honey is made up of 20% water and 80% carbohydrate. Most of them are glucose and fructose. Honey is in a liquid state, but because it contains a large amount of fructose, it absorbs moisture in the air and dissolves itself. In the case of high glucose, it also forms crystals. Sugar is made from raw sugar obtained by heating, precipitation and centrifugation of squeezed juice by squeezing sugar cane and sugar beet with high sugar content or sugar content. The brown sugar and the yellow sugar are produced by refining the raw sugar with little or no purification. And trehalous (trehalous) is widely found in nature such as plants and microorganisms, as well as protection against drying and freezing of food, as well as anti-aging starch. It is known that it has various excellent functions such as prevention of fatty acid corrosion, stabilization of food texture, improvement of taste and flavor. Powdered alcohol is powdered by evaporating water by partial dehydration in short time by adding dextrin to alcohol, suppressing degeneration of starch, inhibiting denaturation of protein, suppressing decomposition of lipid, etc. And sugar alcohols are xylitol, mannitol, sorbitol, erythritol, and galacto-oligosaccharides as non-cyclic linear polyhydric alcohols.
In the present invention, the minerals may be any one or more selected from the group consisting of nephrite, talc, muscovite, sericite, kaolin, seamstone and organic germanium.
Nephrite (nephrite, CaO 4 3 MgO SiO 2) is intertwined with the fine fibrous crystals refers to a compact-to-seomseok or tunok seomseok achieved the gyeonggoe, it said corresponding to jade. The quality is strong and does not break well, and when it is polished, it becomes glossy. The hardness is 5.5 ~ 6.0, the specific gravity is 2.9 ~ 3.0, the color is white and dark green. "It is known that ogal is tasteless and poisonous, it releases the heat of the stomach and intestines, relaxes the osmanthus, and expels waste from the body." Okamu promotes blood circulation and brightens the eyes. " It is known that the clinical effect of nephrite has been shown to be therapeutic effect such as headache, insomnia, burning of mouth and limb and electroacoustic adsorption of anion by Ca 2+ , which is excellent in sterilization action and waste adsorption ability and can prevent acidification of body fluids . The content of nephrite in the cosmetic composition of the present invention is preferably 1 to 10% by weight based on the total weight of the composition. If it is less than 1% by weight, the adsorption force by Ca 2+ becomes weak. If it exceeds 10% by weight, absorption of zinc, magnesium and iron components is interfered.
Talc (talc, Mg 3 Si 4 O 10 (OH) 2) is a rock belonging to the monoclinic system having a crystal structure such as mica, hardness of 1, specific gravity, and can be cut with a knife to 2.7 to 2.8, the color is white, silver white And pale green. Magnesium in talc is known to promote skin metabolism, contribute to the structural stabilization of ATP energy, and promote protein synthesis. Magnesium also has the ability to control contraction and relaxation of muscle contrary to calcium. Talc is mainly used for infant perspiration. It is used to treat eczema because it has the function of eliminating the sausage and reducing the heat. It is used as a base in cosmetics and as an additive in foods and pharmaceuticals. The content of talc in the cosmetic composition of the present invention is preferably 10 to 20% by weight based on the total weight of the composition. If it is less than 10% by weight, the skin metabolism and muscles become too shrunk, and if it exceeds 20% by weight, skin metabolism and muscles become too relaxed.
Muscovite (K (OHF 2 ) 2 Al 3 Si 3 O 10 ) is a monoclinic mineral that contains a small amount of iron and magnesium. The hardness is 2 ~ 2.4, the specific gravity is 2.7 ~ 3, colorless, white, yellow, gray, green, The fluoride component of muscovite is known to inhibit the activation of melanin pigment, which has a whitening effect and moisturizes the skin. The content of muscovite in the cosmetic composition of the present invention is preferably 20 to 35% by weight based on the total weight of the composition. If it is less than 20% by weight, skin whitening and moisturizing effect hardly appear, and if it exceeds 35% by weight, skin whitening and moisturizing effect become excessively excessive.
Sericite (KAl 2 (OH) 2 (AlSi 3 O 10 )) belongs to monoclinic, white or grayish white with pearly luster. Originally referred to as the main component minerals of crystalline schist, especially sericite schist, today refers to fine clay mined muscovite due to hydrothermal action. The chemical composition is almost the same as that of muscovite, but potassium (K) is generally less than muscovite and water (H 2 O) is somewhat higher. In addition, sericite contains various minerals essential for human body such as selenium (Se), zinc (Zn), magnesium (Mg), and calcium (Ca), and it shows heavy metal detoxification and antioxidant efficacy. In addition, sericite is used as a mixture of ceramics and refractory bricks, and there are many other uses such as paints, electric insulators, active materials and cosmetics. The content of sericite in the cosmetic composition of the present invention is preferably 10 to 20% by weight based on the total weight of the composition. If the amount is less than 10% by weight, the detoxification of heavy metals and the antioxidant effect are weakened. If the amount is more than 20% by weight, the intracellular total antioxidant system is destroyed and the detoxification of heavy metals and the antioxidant effect are more weakened.
China clay (kaolin, Al 2 Si 2 O 5 (OH) 4) is kaolinite (kaolinite) and halroyi the site is the main component, feldspar, in the rock o orthoclase o Soda Feldspar o as chairman seats jangseokryu is chemically by acid or water It is produced by decomposition weathering, and is pure white or slightly gray. Kaolin powder cleanses the skin and removes dirt and toxins from the surface of the skin, sebum from the pores, and dead skin cells, and is good for oily and acne skin. In terms of the term, it is called Baekseokji and Hwangseokji according to the color, and they are all used for skin troubles. The content of the kaolin in the cosmetic composition of the present invention is preferably 35 to 45% by weight based on the total weight of the composition. If the content is less than 35% by weight, the affinity between the constituents decreases, and the adsorption force is weakened. If the content exceeds 45% by weight, the affinity becomes too high and the adsorption force becomes too strong.
Pumice is a pore-rich mineral formed by solidification of volcanic rocks. The anatomic rocks are silicate minerals ([SiO 2 ] x ) including rhyolite, trachyte and phonolite, which are essential minerals such as calcium, iron, zinc, copper, nickel, chromium, cobalt, vanadium, . According to the herbal gangmok, the anatomic stone is known to treat the symptom of lump or lump in the skin, the severe lower abdominal pain, and it has the effect of lowering the wrinkles. It is also known to treat the symptoms of blurred vision in the eye. In the cosmetic composition of the present invention, the content of the hydrous stones is preferably 1 to 3% by weight based on the total weight of the composition. If it is less than 1% by weight, it hardly acts on the skin. If it is more than 3% by weight, heavy metals are harmful to the skin.
Organic germanium (Ge) exists in a trace amount, and has an oxygen supplying action, a semiconductor action (an electron flow regulating action, an anion effect), an immunity enhancing action, an interferon production, , The action of administra- tion, the excretion of heavy metals in the body, and the enhancement of natural healing power. Therefore, cosmetics using high purity germanium; Health tools such as bracelets, necklaces, and coats; Soap, and so on, and it is a substance that shows a strong growth rate in demand around the world. In the cosmetic composition of the present invention, the content of the anatomic stones is preferably 0.1 to 0.3% by weight based on the total weight of the composition. If it is less than 0.1% by weight, the effect of germanium does not appear. If it exceeds 0.3% by weight, it shows toxicity to the skin.
Minerals other than organic germanium in the above minerals include essential elements such as Ca, Mg, P and K and elements such as Fe, Zn, Se, Mn, Cu, Mo, Co, Cr, F, Ni, V, B, Se and other micro-elements, which can contribute to the cell's life activity. In particular, selenium among the above elements is a representative biodegradable enzyme that acts to remove hydrogen peroxide generated in the body as a coenzyme of glutathione peroxidase enzyme. Therefore, the elements such as selenium contained in the mineral complex component are known to promote the action of glutathione peroxidase, thereby removing the by-products of intracellular metabolism, and participating in cell division and differentiation.
Silicon (Si, silicon), which is commonly contained in the minerals, is a natural substance contained in uncooked grains. It is an important element accounting for 0.5% of body weight and plays an important role in protein synthesis as a connective tissue So that the connective tissue is an essential component for maintaining elasticity. Silicone also has research on immunity-enhancing functions. In order to use the silicon ingested by cells, various essential minerals such as boron, calcium, magnesium, manganese, and potassium are needed at the same time.
The present invention also relates to a cosmetic composition for preventing skin aging, improving wrinkles and moisturizing the skin, comprising the chrysanthemum matured product as an active ingredient.
The cosmetic composition of the present invention may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, And may be any one selected from the group consisting of.
The cosmetic composition of the present invention may contain, as an active ingredient, components commonly used in cosmetic compositions in addition to the above-mentioned chrysanthemum extracts, and may contain conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, Carrier.
Meanwhile, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, Containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. However, the present invention is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a convergent lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.
When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.
When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, Propane / butane, or dimethyl ether. ≪ RTI ID = 0.0 >
When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate , Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.
When the formulation of the cosmetic composition of the present invention is in the form of a suspension, the carrier component may include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester Suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
When the formulation of the cosmetic composition of the present invention is a cleansing composition containing an interfacial active agent, it is preferable to use, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, Fatty acid amide ether sulfate, alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.
Hereinafter, the present invention will be described in more detail by way of examples. However, these embodiments are provided to aid understanding of the present invention, and the scope of the present invention is not limited to these examples in any sense.
Unless otherwise defined, all technical and scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
(Example 1) After sterilization, 4.5 wt% sugar and 0.5 wt% minerals were mixed and aged for 45 days
4.5 g of sugar and 0.5 g of powdery pomegranate were mixed with 45 g of chrysanthemum and 50 g of purified water at 20 캜 in a sterilization room and then aged for 45 days in sterilized condition.
(Example 2) After mixing 4% by weight of sugar and 1% by weight of minerals in the sterilized state,
4 g of sugar and 45 g of powdered nephrite were mixed with 50 g of purified water at 20 ° C in 45 g of chrysanthemum and sterilized for 45 days in sterilized room.
(Example 3) After sterilization, 3 wt% sugar and 2 wt% minerals were mixed and aged for 45 days
The chrysanthemum purchased from the market was washed well and then dried. In a sterilization chamber, 3 g of sugar and 45 g of powdered nephrite were mixed with 45 g of chrysanthemum and 50 g of purified water at 20 캜 for 45 days in sterilized condition.
(Comparative Example 1) A mixture of 4.5% by weight of sugar and 0.5% by weight of minerals in a non-sterilized state and aged for 45 days
The rest of the procedure was the same except that it was made non-sterile in Example 1.
(Comparative Example 2) 45% by weight of sugar and 5% by weight of minerals were mixed in a non-sterilized state,
The rest of the procedure was the same except that 45% by weight sugar and 5% by weight nephrite were mixed in the non-sterile state in Example 1.
(Comparative Example 3) After sterilization, 0.45% by weight of sugar and 0.05% by weight of minerals were mixed and aged for 45 days
The remaining procedure was the same except that 0.45 wt% sugar and 0.05 wt% nephrite were mixed in Example 1.
(Comparative Example 4) After sterilization, 9 wt% sugar and 6 wt% minerals were mixed and aged for 45 days
The remaining procedure was the same except that in Example 1 9 wt% sugar and 6 wt% nephrite were mixed.
(Comparative Example 5) 45% by weight of sugar and 5% by weight of minerals in a sterilized state,
The remaining procedure was the same except that in Example 1 45 wt% sugar and 5 wt% nephrite were mixed.
(Comparative Example 6) Aging (except for minerals) for 45 days after mixing 5% by weight of sugar in a sterilized state,
The remaining procedure was the same except that only 5 wt% sugar was mixed in Example 1 and nephrite was not mixed.
(Comparative Example 7) After sterilization, 5% by weight of minerals were mixed and aged for 45 days (except for sugar)
The rest of the procedure was the same except that only 5 wt.% Nephrite was mixed in Example 1 and the sugar was not mixed.
(Comparative Example 8) Sterilized for 45 days after mixing with 15 wt% sugar in a sterilized state (minerals excluded)
The remaining procedure was the same except that only 15 wt% sugar was mixed in Example 1 and nephrite was not mixed.
(Comparative Example 9) After sterilization, 15% by weight of minerals were mixed and aged for 45 days (except for sugar)
The rest of the procedure was the same except that only 15% by weight parenthood was mixed in Example 1 and the sugar was not mixed.
(Comparative Example 10) A mixture of 2% by weight of sugar and 3% by weight of minerals in a sterilized state and aged for 45 days
The remaining procedure was the same except that in Example 1 2 wt% sugar and 3 wt% nephrite were mixed.
(Comparative Example 11) A mixture of 1% by weight of sugar and 4% by weight of minerals in a sterilized state and aged for 45 days
The remaining procedure was the same except that 1 wt% sugar and 4 wt% nephrite were mixed in Example 1.
(Comparative Example 12) General hot water extraction
The chrysanthemum purchased from the market was washed well and dried. The mixture was mixed with 50 g of chrysanthemum powder (50 g) at room temperature and mixed with 50 g of purified water at 80 ° C, and the mixture was allowed to stand for one day.
(Comparative Example 13) 30% ethyl alcohol extraction
The rest of the procedure was the same except that 30% ethyl alcohol was used in place of purified water at 80 ° C in Comparative Example 12.
(Comparative Example 14) 50% alcohol extraction
The rest of the procedure was the same except that in Comparative Example 12, 50% ethyl alcohol was used instead of purified water at 80 캜.
(Comparative Example 15) 70% alcohol extraction
The rest of the procedure was the same except that in Comparative Example 12, 70% ethyl alcohol was used instead of purified water at 80 캜.
(Comparative Example 16) 100% alcohol extraction
The rest of the procedure was the same except that in Comparative Example 12, 100% ethyl alcohol was used instead of purified water at 80 캜.
(Test Example 1) Antioxidant activity measurement (DPPH)
The antioxidative activity of the solution isolated from chrysanthemum was measured as follows.
50 uL of each solution of each test group was dispensed into a 96-well plate and 150 uL of 100 uM DPPH (1,1-diphenyl-2-picrylhydrazyl, Sigma, USA) solution dissolved in ethanol was added. After incubation at room temperature for 30 minutes, the absorbance was measured with an ultraviolet / visible spectrophotometer (SPECORD 200, Analytic jeca) at 530 nm using an ELISA reader (DYNEX, USA). As a control, 50 uM L-ascorbic acid (Sigma, USA) was used. The DPPH radical scavenging activity of each sample was calculated in comparison with the absorbance of the control group to which no sample was added. The absorbance was measured and the degree of antioxidant activity was measured according to the following equation.
Antioxidant activity (%) = [1- (sample absorbance 530 nm) / (absorbance 530 nm)] x 100
(Test Example 2) Measurement of cell activity
The macrophage cell line (RAW 264.7) used in the experiment was purchased from Korean cell line bank and the morphology was observed. Cells subcultured 10 times or less were used for the experiment. RAW 264.7 cells were subcultured in a 5% CO2 incubator at 37 ° C in DMEM (Invitrogen, USA) supplemented with 10% (v / v) FBS Respectively.
Cell safety (cytotoxicity)
Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using 3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) reagent. RAW 264.7 cells were seeded at a density of 1 × 10 5 cells / well in a 96-well plate and cultured at 37 ° C and 5% CO 2 for 24 hours. After the culture medium was removed, the sample was incubated in the treated medium for 24 hours, and the medium was removed and washed twice with PBS (phosphate buffered saline). MTT was dissolved in PBS at 300 μg / mL and 100 μL was added and incubated at 37 ° C and 5% CO 2 for 4 hours. DMSO was added to each well of 150 μL and stirred for 30 minutes. Absorbance was measured at 570 nm (reference 600 nm) using an ELISA reader (DYNEX, USA). The degree of cell proliferation was expressed as a percentage of the absorbance of the control.
Promoting collagen biosynthesis
Collagen aggregation performance was assessed by the use of antibodies that recognize the C-terminus of the collagen precursor (PIP, Procollagen Type 1 C-peptide). Cells were treated with 100 ug / mL of each sample at a concentration of 200 ug / mL, and the culture solution was collected to measure the amount of the C-terminus of the collagen precursor.
According to the above results, it was confirmed that the chrysanthemum matured according to the present invention had statistically significant activity against collagen biosynthesis, and thus it was confirmed that the chrysanthemum matured according to the present invention had a remarkably excellent effect for wrinkle improvement .
NF-kB activity inhibitory activity
To determine whether the activity of inhibiting the production of inflammatory cytokines (IL-1β, IL-6 and TNF-α) produced by LPS stimulation in RAW 264.7 cells was related to nuclear factor-kappaB (NF-κB), RAW 264.7 Cells were subcultured in 96-well plates at a density of 5 x 10 cells / well and stabilized for 24 hours. Samples were diluted to 0-0.5 mg / mL and stimulated with 1 ug / mL LPS (Lipopolysaccharide, Sigma, USA) 2 hours later. The expression of NF-κB was measured using a mouse nuclear factor-kappaB p65 ELISA kit (MyBioSource, USA) and a mouse inhibitory subunit of NF-κB (IKBa) ELISA kit (MyBioSource, USA) . The concentration of NF-κB p65 was calculated to 12 ng / mL, and the concentration of IKBα was diluted by 2 fold, compared with the standard curve obtained.
According to the above results, it was confirmed that the chrysanthemums of the present invention had a statistically significant effect on inhibition of NF-kB activity. Thus, the chrysanthemum matured according to the present invention has a remarkably excellent effect for relieving or improving inflammation .
The results of Test Example 1 and Test Example 2 are summarized in Table 1 below.
[Table 1] Measurement results of radical scavenging activity and cell activity
(Test Example 3) Evaluation of effect of cosmetic composition through sensory evaluation
The hue and flavor of the chrysanthemums prepared in Examples 1 to 3 and Comparative Examples 1 to 16 were evaluated by a sample of 40 adult women between 20 and 40 years old.
The evaluation was made using a conventionally used blind-test so that each sample experimenter was unaware of the sample numbers of Examples 1 to 3 and Comparative Examples 1 to 16 to prevent the prejudice of the samples from intervening.
In the evaluation process, each sample was simultaneously supplied to the experimenter. To prevent the influence of the results on the previous sample from affecting the evaluation of the next sample, wash the hands to treat the sample before evaluation of each sample. And the following samples were evaluated.
The evaluation score was 5 points of 5 points for each evaluation item, and the score was divided into 5 points, 5 points for the best, 4 points for the normal, 3 points for the normal, 2 points for the bad, and 1 point for the worse. The mean value for the sample was derived. The results are shown in Table 2 below.
[Table 2] Hue, incense
As shown in Table 2, when Examples and Comparative Examples were compared, in Examples 1 to 3, both color tone and flavor were evaluated. However, in the case of Comparative Examples 12 to 16, only the extracts were extracted with hot water or alcohol only. Experiments were sensitive to chrysanthemum, and the color tone was not so good. In addition, in the case of Comparative Examples 1 to 11, the chrysanthemum fermentation solution obtained in a non-sterilized condition, and in this case also, the evaluation of the hue and flavor of the subject is better than that of the chrysanthemum obtained through the examples of the present invention I did not receive it.
From the above results, it was found that the chrysanthemums obtained by the present invention had excellent antioxidative ability, collagen production ability and NF-kB inhibitory ability, and remarkably decreased cytotoxicity. Also, in the evaluation of the blinded method, And thus the present invention has been completed.
(Formulation Example 1) Cream cosmetic composition 100 g
Chrysanthematous material according to Example 1: 10.0 g
1,3-BG: 10.0 g
Glycerin: 5.1 g
Propylene glycol: 4.2 g
Tocopheryl acetate: 3.0 g
Triethanolamine: 1.0 g
Macadamia nut oil: 2.5 g
Polysorbate 60: 1.6 g
Sorbitan sesqui-citrate: 1.6 g
Propylparaben: 0.6 g
Preservatives: trace
Purified water: Remaining amount
(Formulation Example 2) Skin cosmetic composition 100 g
Chrysanthematous material according to Example 1: 10.0 g
1,3-BG: 10.0 g
Glycerin: 6.1 g
Propylene glycol: 5.3 g
Tocopheryl acetate: 2.0 g
Triethanolamine: 1.0 g
Polysorbate 60: 1.2 g
Sorbitan sesquiterrate: 1.3 g
Propylparaben: 0.1 g
Purified water: Remaining amount
Claims (7)
Wherein the mixture of the saccharide and the mineral is 1 to 10% by weight based on the total weight of the aged material.
Wherein the saccharides and minerals are mixed in a weight ratio of 1: 9: 1 with saccharides: minerals.
Wherein the saccharide is one or more selected from the group consisting of glucose, lactose, fructose, sugar, trehalose, xylitol, mannitol, sorbitol, erythritol and galactooligosaccharide.
Wherein the minerals are at least one selected from the group consisting of nephrite, talc, muscovite, sericite, kaolin, seamstone and organic germanium.
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102018213176A1 (en) | 2017-08-08 | 2019-02-14 | Mando Corporation | Control circuit for a three-phase motor and control method for a three-phase motor |
| KR20200005784A (en) * | 2018-07-09 | 2020-01-17 | 주식회사 크릴랜드 | Red or Gold Ginseng berry Aged solution and manufacturing method |
| KR20200019303A (en) * | 2018-08-13 | 2020-02-24 | 주식회사 제주볼케이노 | Wood cultivated ginseng Matured solution and manufacturing method |
| KR20210002875A (en) | 2019-07-01 | 2021-01-11 | 안명근 | Cosmetic composition for anti-inflametion, anti-wrinkle, whitening and anti-bacterial comprising Extract of Chrysanthemum morifolium |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100819211B1 (en) * | 2006-06-21 | 2008-04-02 | 서희동 | A manufacturing method of the plant ferment-extract |
| JP6490342B2 (en) * | 2014-03-12 | 2019-03-27 | 株式会社再春館製薬所 | Anti-skin aging agent containing Shiranui Chrysanthemum extract |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102018213176A1 (en) | 2017-08-08 | 2019-02-14 | Mando Corporation | Control circuit for a three-phase motor and control method for a three-phase motor |
| KR20200005784A (en) * | 2018-07-09 | 2020-01-17 | 주식회사 크릴랜드 | Red or Gold Ginseng berry Aged solution and manufacturing method |
| KR20200019303A (en) * | 2018-08-13 | 2020-02-24 | 주식회사 제주볼케이노 | Wood cultivated ginseng Matured solution and manufacturing method |
| KR20210002875A (en) | 2019-07-01 | 2021-01-11 | 안명근 | Cosmetic composition for anti-inflametion, anti-wrinkle, whitening and anti-bacterial comprising Extract of Chrysanthemum morifolium |
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