KR20200019303A - Wood cultivated ginseng Matured solution and manufacturing method - Google Patents

Abstract

The present invention relates to an aging solution for wood-cultivated ginseng and a manufacturing method thereof, and more specifically, a method for manufacturing an aging solution for wood-cultivated ginseng by using wood-cultivated ginseng with a high saponin content together with minerals and saccharide. In other words, the present invention relates to an aging solution for wood-cultivated ginseng, or a cosmetic composition includes the aging solution as an active ingredient for regenerating skin, preventing or removing skin wrinkles, preventing or removing skin aging, relieving skin inflammation, or improving skin lightening; and to a manufacturing method thereof. Therefore, the manufacturing method for an aging solution for wood-cultivated ginseng can avoid destruction of active ingredients in wood-cultivated ginseng as much as possible by mixing minerals and saccharide in wood-cultivated ginseng, aging, and extracting the same, and can provide the cosmetic composition for regenerating skin, preventing or removing skin wrinkles, preventing or removing skin aging, relieving skin inflammation, or improving skin lightening by enhancing activities of an enzyme contained in the wood-cultivated ginseng. In addition, the manufacturing method for an aging solution for wood-cultivated ginseng can significantly reduce production costs using minerals and saccharide, can provide excellent a skin lightening effect, a skin regeneration effect, and an antioxidant effect by extracting various phytonutrients of wood-cultivated ginseng.

Description

Goat's ginseng matured solution and its manufacturing method {Wood cultivated ginseng Matured solution and manufacturing method}

The present invention relates to a goat goat aging liquid and a method for preparing the same, and more particularly, to a method for producing a goat goat aging liquid using a mineral powder and a sugar with high saponin content.

Specifically, the present invention is a goat composition for aging or skin aging containing the aging liquid as an active ingredient, to prevent or improve skin wrinkles, to prevent or improve skin aging, to improve skin inflammation or to improve skin whitening cosmetic composition and its preparation It is about a method.

Panax Ginseng ginseng CA Meyer ) is a cultivated seed that is artificially planted with seed or seedling under mountain forests. Goat ginseng is a perennial herbaceous plant belonging to the family arboraceae, and is a representative medicinal crop in Korea that has been widely used for thousands of years. It is very sensitive to environmental factors including soil, and is very demanding in cultivation conditions. However, it is known to have little toxicity and to be effective for all diseases.

According to the Law on Promotion of Forestry and Mountain Village Promotion, revised in July 2011, Sanyang Ginseng is a clean ginseng produced in a natural state without the installation of artificial facilities such as shading barriers in the mountainous areas of Article 2-1 of the Mountain Management Act. It is prescribed. Goat ginseng is a forest product produced in a forest, as it is, in a natural state for a long time (10 to 20 years) and is clean (no pesticides, no premium), and is nurtured and managed to ensure naturalness and cleanliness in the law on forestry and mountain village promotion. Goat's ginseng in the present invention, of course, refers to goat's goat prescribed in the above laws.

Wild ginseng has been called a mysterious elixir since ancient times. The fruit of ginseng excreted in nature by birds and beasts is called wild ginseng, and it is today's wild ginseng that began to cultivate the wild ginseng collected from nature with human hands. Therefore, goat ginseng and wild ginseng are known to have similar efficacy.

Among the 35 types of saponins contained in goat ginseng, the main role of saponins medically revealed in Healthweek magazine is "Rb1 is hepatic and fatigue recovery, Rb2 is diabetes control and obesity and arteriosclerosis, hepatocellular proliferation, Rh1 is liver function improvement, Fatigue recovery, platelet aggregation inhibition, Rh2 is anticancer and immune enhancement, Rg1 is memory enhancement and prevention of dementia, diabetes control, obesity prevention, fatigue recovery, Rg2 is memory enhancement and prevention of dementia, platelet aggregation inhibition, Rg3 is anticancer and anticancer drug resistance Function, immunity enhancement, Re to protect liver, promote bone marrow cell production, enhance memory and prevent dementia, other Rf, Ro, Rd, etc. inhibit lipid peroxidation, brain nerve cell analgesic, alcohol detoxification, inflammation treatment, protein and lipid synthesis And adrenal cortex hormone secretion promoting activity. "

On the other hand, in Heo Jun's 'Consensus Bogam', Sansam is said to be effective in boosting energy, boosting immunity, eliminating toxins in the body, and improving chronic fatigue by improving the function of the human body. In addition, saponins contained in wild ginseng is said to strengthen the disease resistance and improve immunity to a strong constitution.

The wild ginseng grown in the closest way to this wild ginseng is a natural health food that contains ingredients similar to wild ginseng. It is a mysterious food that helps to prevent immunity and develop immunity to modern people who have reduced physical strength.

Among them, Jeju Sanyangsam is cultivated at high altitudes of more than 500m above Halla Mountain, and the western part of the island is cultivated mainly in the western provinces of Jeju Island because of its better habitat than the east of Halla Mountain. Jeju goats are attracting attention because they show better results than other goats in other regions. Jeju goats produce goats that have excellent marketability through excellent habitat and strict quality control.

Saponin, known as the major medicinal ingredient of goat's ginseng, is commonly called ginsenoside. Other medicinal ingredients are nitrogen compounds, which are proteins, amino acids, nucleic acids, alkaloids, and fat-soluble ingredients that are dissolved in oil. Essential oils, polyacetylenes, phenolic compounds, water-soluble vitamins (0.05%), minerals (4-, etc. and carbohydrates contain monosaccharides and polysaccharides) (Rural Development Administration).

Existing goat ginseng extract is prepared by a conventional method known in the art, that is, using a conventional solvent under the conditions of the usual temperature and pressure, a general extraction method, separation and purification methods known in the art are used. . In addition, methods such as hot water extraction, hot water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction are used. However, this manufacturing method has the disadvantage of increasing the cost of energy consumption, low stability of the process.

And microbial (lactic acid bacteria) method, acid and enzyme hydrolysis method, etc. The microorganism (lactic acid bacteria) method has the advantage that can selectively strengthen the specific saponin, but the selection and culture technology of lactic acid bacteria It is difficult, and the process is complicated and difficult to manage, the hydrolysis method is different in the selectivity or conversion efficiency of the specific saponin according to the type of acid or enzyme, and generally selectivity or conversion than the method by the microorganism The disadvantage is that the efficiency is somewhat lower.

Prior art Republic of Korea Patent No. 10-0824285 describes the invention for the saccharified ginseng and ginseng extract, including the process of fermentation with lactic acid bacteria or enterobacteriaceae, as described above the method by the microorganism is selected and cultured The problem is that the technology is difficult, the process is complex and difficult to manage.

Fermentation and maturation may be mixed in the same concept in the study of natural products, but fermentation and maturation differ in the process.

Fermentation is inoculation of natural microorganisms with specific microorganisms, such as yeast or bacteria, to obtain the metabolites of microorganisms in which natural ingredients are replaced with other ingredients or ingested natural ingredients by the action of microorganisms. It produces a product and can detect the process of fermentation because the fungus is visible. As a representative example of fermentation, when boiled soybeans are placed on rice straw and left for 2 to 3 days at 40-50 ° C., the Bacillus subtilis of rice straw breaks down the protein of soybean to produce glutamic acid and produces sticky viscous substance.

On the other hand, aging is to leave the natural product for a while under certain conditions to synthesize or change the active ingredient by the interaction of the enzyme or the component of a protein, such as a gene present in the natural product. This decreases or increases, and this ripening process is often not visible to the naked eye. As an example of aging, if the seed is aged for 6 to 12 months after heat sterilization, irritating aldehydes are reduced and esters are increased to improve taste.

Therefore, the present invention invented a method of extracting an effective phytonutrient of goat goat by osmotic pressure and ripening using minerals and sugars without using a fermentation method using fungi or fermentation broth.

The present inventors have completed the present invention to increase the useful value as a functional product by extracting a variety of phytonutrient (phytonutrient) of goat ginseng as a manufacturing method to significantly lower the production cost using minerals and sugars.

An object of the present invention is to prepare a mature goat ginseng liquid, specifically, after extraction by mixing and ripening the minerals and sugars in the goat ginseng, not only the active ingredients of goat ginseng are not destroyed as much as possible, the activity of the enzyme contained in goat ginseng It is to provide a cosmetic composition for increasing skin regeneration, preventing or improving skin wrinkles, preventing or improving skin aging, improving skin inflammation, or improving skin whitening.

It is another object of the present invention to provide a method for producing a goat's ripening liquid excellent in whitening, skin regeneration and antioxidant effects by extracting various phytonutrients of goat's ginseng as a method of significantly lowering the production cost using minerals and sugars. It is.

As one embodiment for solving the above problems, the present invention provides a composition for improving the skin condition containing a goat goat aging liquid or an active ingredient thereof.

The present inventors devised an aging system for activating enzymes using minerals and sugars to produce more efficacious ingredients contained in natural products at the time of natural aging. (MSA method). The aging method is not only high efficacy by activating the metabolism of the natural product as it is without any organic solvent or artificial manipulation, it is possible to obtain a safe raw material having a new effect and function not seen in the existing natural product.

In order to obtain the above raw material, the present invention, as one embodiment, goat ginseng ripening liquid is characterized in that the extract is prepared by aging by mixing minerals and sugars in goat ginseng. The mineral and sugar are mixed with goat ginseng to improve the activity of the enzyme to increase the yield of the main bioactive material of goats.

All living organisms have a variety of enzymes that catalyze biochemical reactions. Since the enzyme is composed of proteins, it shows all the properties of the protein, and unlike inorganic catalysts, it is greatly influenced by environmental factors such as temperature, pH (hydrogen ion concentration), protein denaturant, and loses its activity when the steric structure is irreversibly denatured. Also do. That is, all enzymes are most active under certain conditions, and one enzyme catalyzes one chemical reaction or a similar group of chemical reactions, does not cause rejection and produces no byproducts.

By physiological activity in these organisms, plants secrete phytoalexin, which is a plant that synthesizes phytoalexin itself as part of its defense mechanism when the plant is in an adverse environment. Plants can increase their resistance to disease by producing antibacterial substances called phytoalexins as an important means of defense against invaders and protecting themselves against germs.

In the present invention, the method for preparing goat goat aging liquid is ginseno, which is the main bioactive substance of goats ginseng due to the osmotic pressure caused by minerals and sugars, without adding an organic solvent or applying heat in the process of obtaining an effective ingredient contained in goats ginseng. Ginsenoside, non-saponinic acidic polysaccharide, panaxan, adenosine, pyroglutamic acid, acidic peptide, polyacetylenes, phenolic compounds compounds) and other phytonutrients can be easily produced and eluted and obtained in concentrated form without destroying as much as possible.

The phytoalexin is specifically synthesized by goats in the present invention, when the goats are placed under stress conditions, specifically, when the goats are placed under osmotic conditions by minerals and sugars, they are strengthened from goats. Ginsenoside, a major bioactive substance of goat ginseng, acidic polysaccharides of non-saponins, panaxan, adenosine, pyroglutamic acid, acidic peptide, poly Polyacetylenes, phenolic compounds, and other valid phytonutrients are fortified with phytoalexin. The phytonutrient (phytonutrient) is a physiologically active substance contained in vegetables and fruits to inhibit free radicals attacking cells in the body through a powerful antioxidant action and functions to treat and prevent intracellular DNA damage.

In the present invention, goat ginseng is most preferably based on Jeju goats produced in Jeju in Korea, goats are not limited to this, and all types of goats may be defined as goats of the present invention.

The present inventors confirmed the effects of the extract by measuring the cell activity, skin regeneration effect, melanin production inhibition, etc. by extracting the aging of minerals, sugars and goat ginseng without any extraction solvent.

The minerals include various complex mineral components of any one or more of Ca, Mg, Cu, Zn, Na, K, Fe, P, Cl, Ge, Pt, Se, and especially magnesium, calcium, which are four essential minerals necessary for the human body. In addition to potassium, sodium, zinc and selenium, as well as potassium, phosphorus and other inorganic nutrients necessary for plant growth, stabilizing goat ginseng, and acts as a catalyst to promote the activity of various enzymes. The minerals catalyze the production and elution of phytonutrient components, which are the major bioactive substances present in goat ginseng. The content of the mineral is preferably 0.1 to 50% by weight, more preferably 1.0 to 30% by weight, most preferably 5.0 to 15% by weight relative to the total weight of the mixture of sugars and minerals.

The sugar may be selected from any one or more of sugar, honey, sucrose, glucose (glucose), allulose, tagatose, starch syrup, oligosaccharide, fructose, glucose, preferably Preferably, any one or more of sugar, honey, sucrose (sucrose) or allulose or oligosaccharides may be selected and used.

The saccharide of the present invention destroys the cell walls of the goats by osmotic pressure so that phytonutrient components, which are the main physiologically active substances contained in goats, are produced and eluted, thereby preventing contamination by rot bacteria. The mixing ratio of the sugar and goat ginseng is preferably a weight ratio of 15: 85 to 70: 30, more preferably a weight ratio of 25: 75 to 50: 50, and most preferably 30: 70 to 35: 65 weight ratio.

When the mixing ratio of the sugar and goat ginseng is outside the weight ratio of 15: 85 to 70: 30, the sugar content is insignificant and the phytonutrient component of the goat may not be easily produced and eluted or contaminated by decayed bacteria. .

If the sugar is 25% or more relative to the water, the sterilization effect starts to appear, thereby obtaining the effect of the aging process rather than fermentation. When the ratio of sugar is low and the fermentation is made, it is determined that the fermentation process using the mineral sugar of the present invention proceeds to aging, there is a problem that all materials must be discarded. As described above, the present invention should proceed to aging, not fermentation.

When sugar exceeds 70% of moisture, excessive sugars inhibit the activity of enzymes present in goat ginseng, reducing the production rate of phytonutrient components of goat ginseng and decreasing the melanin production inhibitory effect of goat ginseng matured liquid. have.

However, the goat ginseng of the present invention is not only made of water, and the total weight is measured including other substances, and therefore, the mixing ratio of the sugar and goat ginseng is preferably based on a weight ratio of 15:85 to 70:30.

In the present invention, in order to confirm the optimum aging conditions using minerals and sugars, the tendency of aging according to the content of used minerals and the concentration of goat ginseng extract was confirmed. On the basis of the maturation period of 20 weeks, the mineral content was tested by applying 0.01 to 70% by weight relative to the total weight of the sugar and mineral mixture.

When the content of the mineral in the aging process of 0.1 to 50% by weight, it was confirmed that the skin regeneration effect is gradually increased. When the content of the mineral is 5.0 to 15% by weight, the most excellent effect was obtained, and the effect decreased as the amount approached 0.01 to 70% by weight out of 0.1 to 50% by weight. Therefore, it was confirmed that the mineral content based on the efficiency using goat ginseng and sugars is most appropriately mixed at 0.1 to 50% by weight relative to the total weight of the mixture of sugars and minerals.

When the content of the mineral is less than 0.1% by weight, the mineral content is insignificant, the effect of improving the activity of the enzyme in goat ginseng is insignificant, and the degree of generation and elution of the effective phytonutrient component of goat ginseng is lowered. The effect of wrinkle improvement is lowered.

When the content of the mineral is more than 50% by weight, the mineral content is excessive, the production and elution of the phytonutrient component of goat ginseng due to the osmotic pressure of saccharides is reduced, and the effect of wrinkle improvement of the goat ginseng ripening liquid is somewhat reduced. There is a decreasing problem.

Hereinafter, a method for preparing a goat ginseng ripening liquid of the present invention will be described in detail.

The method for producing a goat's ripening liquid of the present invention

Washing and drying the goats (dehydrated) (S10);

In step (S10) to wash and drained goat ginseng (optimum conditions) sugar and minerals by mixing uniformly and then aged to prepare a goat ginseng aging liquid (S20);

And eluting the goat ginseng ripening liquid of the step (S20) (S30).

In addition, the method for producing a goat ginseng ripening liquid of the present invention may further include a step (S40) for 1 to 4 weeks after performing the step (S30).

First, the step (S10) to wash the goat. In the step (S10) after washing the goats with purified water to remove foreign substances, only remove the water attached to the surface of goats. At this time, it is preferable not to apply heat so that moisture contained in the goat.

Next, in step (S10) to perform a step (S20) to prepare a goat ginseng aging liquid by mixing the goat and ginseng removed and drained (optimum conditions) with sugars and minerals. Goat ginseng and saccharides of the step (S10) 30: 70 ~ 85: 15 weight ratio, preferably 50: 50 ~ 75: 25 weight ratio, more preferably 65: 35 ~ 70: 30 weight ratio of the enzyme and at the same time Minerals that govern activity are mixed in an amount of 0.1-50% by weight, preferably 1.0-30% by weight, more preferably 5.0-15% by weight, based on the total weight of the mixture of sugars and minerals. At this time, the step (S20) is preferably carried out during the ripening period of 20 to 24 weeks. If the content of sugar is more than 70, the dissolution effect may be rather easy, but rather, the activity of Enzyme is lowered and the effect tends to be lower than the optimal condition, whereas if it is less than 15, it is contaminated with microorganisms and fermentation or decay occurs. According to the results of application experiments according to the ratio under the premise that the active enzymes of goats ginseng are not the same, as shown in the efficacy results, the overall efficacy of goats ginseng is higher when the mineral content is higher. there was. Therefore, the ratio of minerals is less than 0.1% of the sugars applied, shows little effect on the activity of the enzyme during the aging process, when the excess of more than 50% is also not aging smoothly, the efficacy and application to cosmetic formulations It does not have the adverse effect.

The present invention is applied to determine the optimum ripening period using minerals, sugars and goats ginseng, the content of minerals is applied to 0.1 ~ 50% by weight relative to the total weight of the mixture of sugars and minerals, weekly aging state and The tendency of the skin regeneration effect was confirmed. As a result of confirming the aging trend for 48 weeks and about 1 year, it was confirmed that the skin regeneration effect increased as the concentration of goat goat aging solution increased regardless of the aging period.

On the other hand, if the ripening period is less than 20 weeks, the ripening period is too short, so that the enzyme and phytonutrient components are not eluted in the goat ginseng ripening liquid. The increase of the effect was confirmed to be insignificant. That is, the time point at which the goat goat aging liquid of the present invention reaches the optimum effect may be 20 to 24 weeks, and since there is no contamination by the bacteria in the aging process, the goat goat aging liquid is deteriorated even after aging for more than 24 weeks. There was no problem. However, if more than 24 weeks, the yield of enzymes and phytonutrient components is slowed down, which is economically inefficient.

When the maturation period is 20 to 24 weeks, the antioxidant and antiwrinkle effect of the goat's goat aging solution appears best. When the fermentation period exceeds 24 weeks, the yield of goat ginseng matured eluate does not increase significantly compared to the increase in fermentation period, and the antioxidative and antiwrinkle effect of the goat ginseng matured solution is insignificant.

The step (S20) is preferably carried out at a temperature of 20 to 40 ℃, when the enzyme of the goat and phytonutrient (phytonutrient) components of goats at a temperature of 20 to 40 ℃ produced and eluted without deterioration or destruction Antioxidant and antiwrinkle effect of the aging solution is the best. When the temperature of the step (S20) is more than 40 ℃ saccharides are deteriorated during aging, the phytonutrient component of goats ginseng is not sufficiently produced and eluted, and the effect of antioxidant and wrinkle improvement of goats aging liquid is reduced. If the temperature of the step (S20) is less than 20 ℃ temperature is too low to ripen, there is a problem that the elution of enzymes and phytonutrient components of goat ginseng is not properly made.

Performing step (S30) to elute the goat ginseng ripening liquid of the step (S20). The step (S30) may be carried out using a pressure filter or a reduced pressure filter, preferably may be performed using a reduced pressure filter.

After performing the step S30, the step S40 of aging for 1 to 4 weeks may be further performed. At this time, the step (S40) is preferably carried out in a closed container for 1 to 4 weeks. Antiaging and whitening effect of goat ginseng aging liquid is insignificant if aging is less than 1 week in the step (S40), and if aged more than 4 weeks, antioxidant and whitening effect of goat ginseng aging liquid due to excessive ripening There is a problem that decreases.

Goat ginseng ripening liquid of the present invention is applicable to various fields, it may be included in the cosmetic composition in detail.

The cosmetic composition can be prepared in various forms of formulation, can be appropriately selected according to the desired, in detail, flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing It may be prepared in the form of a water, pack, spray, powder or lipstick.

In addition, the cosmetic composition may include other components blended with a conventional cosmetic composition as needed. For example, it may contain a humectant, an organic or inorganic pigment, a ultraviolet absorber, an antioxidant, a pH adjuster, an alcohol, a pigment, a perfume, and the like.

In the present invention, in the use of minerals and sugars of natural cultivated wild ginseng (Wood cultivated ginseng), by maximally activating enzymes (Enzyme) in the plant body by optimal catalytic action and osmotic pressure, the main bioactive substance containing goats ginseng Ginsenosides, acidic polysaccharides of non-saponins, panaxan, adenosine, pyroglutamic acid, acidic peptides, polyacetylenes, phenolic compounds ( phenolic compounds) and other effective phytonutrient ingredients to be produced and eluted, and may be usefully used in cosmetic compositions using the goat goat aging liquid.

The present invention provides, as one embodiment, a goat ginseng aging liquid and a method for preparing the same, which increase skin regeneration effect and the activity of keratinocytes, including aging goat ginseng using minerals and sugars.

Aging method of processing goat ginseng using sugar and mineral of the present invention is a new attempt, goat ginseng maturing liquid of the present invention is different from the existing goat ginseng extract through the aging method of the present invention, the antioxidant effect, intracellular antioxidant and high cell It has a protective effect, a very good melanin production inhibitory effect, and an intracellular antioxidant effect that not only whitens but also improves wrinkles.

Figure 1 is a graph showing the cell growth effect of goat ginseng aging solution (P.Ginseng MSA), Samyang ginseng extract (P.Ginseng Ext).
Figure 2 is a graph showing the antioxidant effect of the goats aged (P.Ginseng MSA), Samyang ginseng extract (P.Ginseng Ext).
Figure 3 is a graph showing the melanin production inhibition rate of goat ginseng aging solution (P.Ginseng MSA), Samyang ginseng extract (P.Ginseng Ext).

Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples and Experimental Examples. These Examples, Comparative Examples and Experimental Examples are for illustrating the present invention, but the scope of the present invention is not limited thereto.

Example  One. Goat's ginseng  Aging liquid (P. Ginseng MSA Manufacturing

Using the following manufacturing method, a goat goat aging liquid was prepared.

Step S10: After washing the goats with purified water to remove foreign substances, only the moisture attached to the surface of goats were removed.

S20 step: Goat ginseng and sugar of step S10 are mixed in a weight ratio of 3: 1, and mixed with minerals 10% by weight relative to the total weight of the mixture of sugar and minerals, and then aged 20 weeks at room temperature 20-30 ℃ A mature eluate was prepared.

Step S30: The goat ginseng of step S20 was extracted with a vacuum filter to prepare a goat ginseng aging liquid (P.Ginseng MSA).

S40 step: Goat aging liquid of the S30 step was further aged for 4 weeks.

Comparative example  One. Goat's ginseng  Extract (P. Ginseng Ext Manufacturing

Goats ginseng was washed with purified water to remove foreign substances, only the water attached to the surface of goats ginseng was removed, and then extracted with a reduced pressure filter using 30% EtOH as an extraction solvent to prepare a goat ginseng extract of Comparative Example 1 (P.Ginseng Ext) It was.

Experimental Example  1. Cell growth effects Cell growth effect )

Cell growth effect test through fibroblasts was carried out through skin fibroblasts.

Samples of different concentrations were prepared by diluting Goat Ginseng MSA and Goat Ginseng Ext of Example 1 and Comparative Example 1 in purified water on Human Dermal Fibroblasts adult (HDFa cells). Inoculated and incubated at 37 ℃ for 3 days to observe the growth effect of the cells.

The concentration of the sample diluted in purified water (% by volume) (Example 1)
Cell Growth Effect of P.Ginseng MSA (Comparative Example 1)
Cell Growth Effect of P.Ginseng Ext One 160.0% 117.0% 0.5 151.0% 121.0% 0.25 135.0% 112.0% 0.12 121.0% 102.0% 0.06 112.0% 101.0% 0.03 101.0% 96.0%

As a result, it was confirmed that goat ginseng aging solution (P.Ginseng MSA) showed a higher cell growth effect than the general solvent extract (P.Ginseng Ext).

As shown in Table 1, goat ginseng aging liquid (P.Ginseng MSA) showed a high cell growth effect at low concentrations in general, whereas general solvent extracts appeared only in the high concentration region of the preceding, almost ineffective. Could confirm.

Therefore, goat ginseng ripening liquid shows a high cell growth effect, and accordingly, high skin regeneration effect of goat ginseng ripening liquid can be inferred.

Experimental Example  2. Antioxidant effect in cells

All cells in the human body are constantly oxidizing due to internal and external factors, causing aging of the cells. Therefore, the antioxidant effect of the cells is also a way to prevent aging. In this experiment, it was confirmed how goats aged (P.Ginseng MSA) and goatsax extract (P.Ginseng Ext) prevented the oxidation of these cells.

Primary cultured human dermal fibroblast (HDFN) and DCFH-DA (2,7-dichlorodihydroflourescein diacetate) were used to determine the effect of the sample on the antioxidant activity in cells. Specifically, HDFN was precultured for 1 day, and then diluted samples were added to each concentration for 1 day. Discard the culture medium, washed twice with PBS, replaced with fresh medium containing 2.5uM DCHF-DA and reacted for 30 minutes. After washing with PBS again, the fluorescence was measured (495nm, 525nm) to investigate the antioxidant capacity of the cells.

The concentration of the sample diluted in purified water (% by volume) Example 1
Intracellular Antioxidant Effect of P.Ginseng MSA (Comparative Example 1)
Intracellular Antioxidant Effect of P.Ginseng Ext One 38.0% 6.0% 0.1 12.0% 2.0% 0.01 7.0% 0.5% 0.001 5.0% 0.1%

As a result, it was confirmed that goat ginseng aging solution (P.Ginseng MSA) showed a much higher antioxidant activity, that is, antioxidant effect than goat ginseng extract (P.Ginseng Ext) using a general solvent.

As shown in Table 2, unlike the conventional solvent extraction goat ginseng goat goat aging solution has a high intracellular antioxidant effect of about 40%, it was confirmed that the effect is maintained to some extent even in the low concentration region.

Based on these results, it was possible to directly confirm the anti-aging effect at the cellular level.

Therefore, goat ginseng aging solution has a high intracellular antioxidant effect, and thus the anti-aging effect of goat ginseng aging solution can be inferred.

Experimental Example  3. Whitening effect

In order to determine the whitening effect of the sample, the melanin production inhibitory effect using melanocytes was confirmed. The cell-line used for the test was B16F10 Melanoma derived from mouse tumor cells of C57BL6, and the test method used a whitening method commonly used in the industry. Specifically, 135 μl of a subcultured B16F10 cell-line adjusted to 5 × 10 ⁴ cells / mL was put into 96well and incubated for 24 hours. Then, the supernatant was added to a new 96well and the absorbance at 490 nm was measured to investigate the melanin production inhibitory effect.

Concentration of sample diluted in purified water (% by volume) Example 1
Inhibition of Melanin Production by P.Ginseng MSA (Comparative Example 1)
Inhibition of Melanin Production by P.Ginseng Ext One 47.0% 18.0% 0.50 36.0% 8.0% 0.25 19.0% 3.0% 0.12 7.0% 1.0%

As a result, goat ginseng aging solution (P.Ginseng MSA) showed a significantly higher melanin production inhibition rate than goat ginseng extract (P.Ginseng Ext) using a common solvent.

P.Ginseng MSA showed more than a certain effect even at low concentrations, whereas P.Ginseng Ext decreased significantly as the concentration decreased, and the effect was low at low concentrations.

Therefore, goat ginseng ripening liquid has a high melanin production inhibitory rate, thereby inferring the whitening effect of goat ginseng ripening liquid.

conclusion

Goat ginseng extract through aging was confirmed to have higher cellular activity than goat ginseng extract obtained under general solvent extraction conditions, and through this, it was confirmed that a component having excellent skin regeneration, anti-aging and whitening effects was obtained.

It was confirmed that the efficacy of goat ginseng aging solution (P.Ginseng MSA) has a cell growth effect.

Goat ginseng aging solution (P.Ginseng MSA) was confirmed to have an intracellular antioxidant effect.

It was confirmed that goat ginseng aging solution (P.Ginseng MSA) had melanin production inhibitory effect.

The efficacy of P.Ginseng MSA has invented a dual functional material that can have skin regeneration effect, anti-aging effect and whitening effect based on cell growth effect, high intracellular antioxidant effect and melanin production inhibitory effect. Confirmed.

Aging method of processing goat ginseng using the sugar and mineral of the present invention is a new attempt, goat ginseng maturing solution of the present invention is different from the existing goat ginseng extract through the aging method of the present invention, cell growth effect and intracellular antioxidant effect, Melanin production inhibitory effect has not only skin regeneration effect but also anti-aging effect and whitening effect.

Claims (8)

Washing and drying the goats (dehydrated) (S10);
In step (S10) to prepare the goat ginseng matured solution by mixing and uniformly mixing the goat and ginseng removed by washing and draining the sugar and minerals (S20);
Extracting the mature goat ginseng of the step (S20) (S30); And
After the step (S30) after the step of aging for 1 to 4 weeks (S40) further comprising the method for producing a goat goat aging liquid, characterized in that it further comprises. The method of claim 1,
The sugar and goat ginseng is a 15: 85 ~ 70: 30 method of producing a goat goat matured liquid, characterized in that mixed in a weight ratio. The method of claim 1,
The mineral is a method for producing a goat goat aging liquid, characterized in that mixed with 0.1 to 50% by weight relative to the total weight of the mixture of sugars and minerals. The method of claim 1,
The ripening period of the step (S20) is a method for producing a goat goat aging liquid, characterized in that 20 to 24 weeks. The method of claim 1,
The sugars are sugar, honey, sucrose (sucrose), glucose (glucose), allulose (Allulose), tagatose (Tagatose), starch syrup, oligosaccharide, fructose, characterized in that it comprises any one or more of the glucose Method for producing a goat's ripening liquid. The method of claim 1,
The mineral is Ca, Mg, Cu, Zn, Na, K, Fe, P, Cl, Ge, Pt, Se production method of goat goat aging liquid, characterized in that it comprises any one or more minerals. Goat ginseng maturing liquid prepared by the method of any one of claims 1 to 6. A cosmetic composition comprising a goat's aging liquid prepared by claim 7.

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