KR102229482B1 - Eryobotrya japonica leaf mineral sugar aging solution having a skin protecting effect and a method for producing the same - Google Patents

Abstract

The present invention is a mixture of minerals and sugars in loquat leaves, ripening and extraction, the active ingredients of loquat leaves are not destroyed as much as possible, and the antioxidant effect by increasing the activity of enzymes present in the leaves of loquat trees in a living state. , It relates to a fermented liquid per mineral sugar of loquat tree leaves having a protective effect against irritation, a skin protective effect having a soothing effect and an anti-pollution effect, and a method for preparing the same.

Description

Eryobotrya japonica leaf mineral sugar aging solution having a skin protecting effect and a method for producing the same}

In the present invention, the active ingredients of the loquat tree leaves are not destroyed as much as possible by mixing minerals and sugars in the leaves of the loquat tree, and the activity of the enzymes present in the leaves of the loquat tree in a living state is increased. It relates to an aging liquid per mineral sugar of loquat leaves having an antioxidant effect, a protective effect against irritation, a soothing effect and an anti-pollution effect, and a method for preparing the same.

Entering the modern society, people have been exposed to various harmful environments and the skin of the face, hands, and body has weakened, and research on substances that have antioxidant effects on the skin, protective effects against irritation, soothing effects and anti-polluting effects. It continues.

As a substance having an antioxidant effect, a protective effect against irritation, a soothing effect and an anti-pollution effect on the skin, there are organic compounds prepared using organic chemistry and natural compositions prepared from natural raw materials. Organic compounds have limitations due to manufacturing cost and finiteness of resources, so studies of natural compositions having antioxidant effects, protective effects against irritation, soothing effects and anti-polluting effects on the skin are actively underway.

As a natural composition that has an antioxidant effect, a protective effect against irritation, a soothing effect and an anti-pollution effect on the skin, a composition prepared using plant extracts is in the spotlight, and research is actively underway.

Among them, loquat trees are in the spotlight, and various studies are being conducted. The Loquat Tree ( Eriobotrya japonica ( Thunb .) Lindl . ) Is a plant of the genus Rosaceae Loquat, and is a small tall, evergreen 3 to 5m tall tree grown mainly for ornamental purposes. The yellow fruit is used for food. Leaves are called loquat leaves and seeds are called loquats, and when water is added to destroy them, the scent of benzaldehyde is produced.

As a conventional technology having a soothing effect on the skin and protecting against irritation by using the loquat tree, Korean Patent No. 10-1566320 includes "Bongseonhwa, Yeongsil, Maca, Loquat tree leaf and yambin root complex fermentation extract. A cosmetic composition for skin rejuvenation, soothing and irritation has been announced, which is made by fermenting a complex extract containing loquat leaves into black oyster fungi to have a skin soothing and irritating effect.

The Republic of Korea Patent Registration No. 10-1566320 shows a feature of fermentation of complex extracts using black tiger mycobacteria.

On the other hand, in the study of natural products, fermentation and aging are sometimes mixed under the same concept, but fermentation and aging are different in the process.

Fermentation is to inoculate a natural product with specific microorganisms such as yeast or fungi, and by the activity of the microorganism, the component of the natural product is changed to another component or the metabolite of the microorganism that ingests the component of the natural product is obtained. It produces a product, and the process of fermentation can be detected because the action of the fungus is visible. As a representative example of fermentation, boiled soybeans are placed on rice straw and left for 2 to 3 days at 40-50°C. Bacillus bacillus in rice straw decomposes the protein of soybeans to produce glutamic acid and produces a thread-shaped sticky viscous material.

On the other hand, aging is the synthesis or change of active ingredients by the action of enzymes, a kind of protein such as genes present in natural products, or interactions between components by leaving natural products under certain conditions for some time. This decreases or increases, and this aging process is often not observed with the naked eye. As an example of ripening, if the sperm is heat-sterilized and then aged for 6 to 12 months, irritating aldehydes decrease and esters increase, thereby improving taste.

Therefore, the composition of the Korean Patent Registration No. 10-1566320 proceeds by fermentation rather than the process of maturation, and the ingredients inside the loquat tree leaves change during the fermentation process, so that the antioxidant effect, the protective effect against irritation, and the soothing effect There was a problem in that it was difficult to take a result showing an appropriate efficacy for.

In addition, since there was no conventional technology dealing with the anti-polluting effect using loquat wood, there was no known technology for having anti-polluting effect using loquat wood.

In accordance with the above-described problems, the need for raw materials having antioxidant effects, protective effects against irritation, soothing effects and anti-polluting effects has increased as they are obtained by applying a new method to loquat leaves.

Therefore, in the present invention, without using a fermentation method using fungi or fermentation broth, a method of extracting an effective phytonutrient from loquat leaves by osmotic pressure and aging using minerals and sugars was invented.

This inventor extracts various phytonutrients from loquat leaves with a manufacturing method that significantly lowers the production cost compared to efficiency by using mineral powders and sugars, and this invention can increase the value of utilization as a functional product by checking its efficacy. The invention was completed.

Republic of Korea Patent Registration No. 10-1566320 (announced on November 5, 2015), "Cosmetic composition for skin regeneration, soothing and alleviating irritation, including complex fermented extracts of Bongseonhwa, Youngsil, Maca, Loquat leaves and Yambin roots"

In order to solve the above problems, the present invention is a mixture of minerals and sugars in a loquat tree leaf, ripening and extraction, so that the active ingredients of the loquat tree leaves are not destroyed as much as possible, but also exist in the leaves of the loquat tree in a living state. The purpose of this is to provide a fermented solution per mineral of loquat tree leaves that has an antioxidant effect, a protective effect against irritation, a soothing effect and an anti-pollution effect on the human body by increasing the activity of the enzyme.

Another object of the present invention is a manufacturing method that significantly lowers the production cost compared to the efficacy that is difficult to expect from a general extract using minerals and sugars. By extracting various phytonutrients from the leaves of the loquat tree, it has an antioxidant effect on the human body and protection against irritation. It is to provide a method for preparing a fermentation solution per mineral sugar of loquat tree leaves having excellent skin protection effect with excellent effect, soothing effect and anti-pollution effect.

In order to solve the above problems, the present invention comprises the steps of washing and drying loquat leaves (S10); Step (S20) of preparing a fermented solution per mineral of a loquat tree leaf having a skin protection effect by aging after uniformly mixing sugars and minerals into the leaves of the Loquat tree washed and removed from moisture in the step (S10) (S20); The step (S30) of producing a filtrate of ripening loquat leaves by compressing and filtering the fermented solution per mineral of loquat tree leaves having a skin protection effect of the step (S20) (S30); And skin protection, characterized in that it has an antioxidant effect, skin protection effect, skin soothing effect and anti-pollution effect, including the step (S40) of eluting the filtrate of the loquat leaves of the step (S30) for 1 to 2 weeks. It provides a method for preparing a fermentation solution per mineral of loquat tree leaves having an effect.

In addition, the mineral is characterized in that it contains any one or more minerals of Ca, Mg, Cu, Zn, Na, K, Fe, P, Cl, Ge, Pt, Se, Bi, Te, Mo, and Nb.

In addition, the saccharides are sucrose, glucose, allulose, tagatose, honey, starch syrup, oligosaccharides, and fructose. It is characterized.

In addition, the mineral is characterized in that it is mixed in an amount of 0.2 to 40% by weight based on the total weight of the mixture of sugars and minerals.

In addition, the present invention provides an aged solution per mineral of loquat tree leaf having a skin protective effect, characterized in that it has an antioxidant effect, a skin protective effect, a skin soothing effect, and an anti-pollution effect, manufactured by the above manufacturing method.

In the present invention, in the use of minerals and saccharides in the natural product of loquat tree leaves, the main physiologically active substances contained in the loquat tree leaves are matured by maximizing the activation of enzymes in the body through the optimal catalytic action and osmotic pressure action. Not only is it produced smoothly in the process, but it can be effectively used in cosmetic compositions by allowing it to be efficiently eluted.

In one aspect, the present invention may exhibit an antioxidant effect on the human body, a protective effect against irritation, a soothing effect and an anti-polluting effect, including the step of ripening a loquat tree leaf using minerals and sugar.

The aging method of processing loquat leaves using sugar and minerals of the present invention is a new attempt, and the aging solution per mineral sugar of loquat leaves having skin protection effect of the present invention is an extract of existing loquat leaves through the aging method of the present invention. It has an antioxidant effect on the human body, a protective effect against irritation, a soothing effect and an anti-pollution effect on the human body, and has a numerically higher wrinkle improvement and skin irritation protection effect.

Figure 1 is a graph showing the antioxidant effect of the fermentation liquid (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat leaves having a skin protective effect.
Figure 2 is a graph showing the cell stability of the fermented loquat leaf mineral sugar (Bio-Liqu) and general loquat leaf extract (Liqu.ext) having a skin protective effect.
Figure 3 is a graph showing the cell-protecting effect as a stimulant source of the fermentation solution (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat leaves having a skin protective effect.
Figure 4 is a photograph showing the skin soothing effect of the aging liquid (Bio-Liqu) per mineral of loquat leaves having a skin protective effect.
Figure 5 is a photograph showing the skin soothing effect of the fermentation solution (Bio-Liqu) per mineral of loquat leaves having a skin protection effect
Figure 6 is a graph showing the anti-pollution effect of the fermentation liquid (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat leaves having skin protection effect.

The following detailed descriptions of the present invention are embodiments in which the present invention may be practiced, and reference is made to the accompanying drawings shown as examples of the corresponding embodiments. These embodiments will be described in detail enough for those skilled in the art to practice the present invention. It should be understood that the various embodiments of the present invention are different from each other, but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention in relation to one embodiment. In addition, it should be understood that the location or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description to be described below is not intended to be taken in a limiting sense, and the scope of the present invention is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims, if appropriately described. Like reference numerals in the drawings refer to the same or similar functions over several aspects.

Terms used in the present invention have selected general terms that are currently widely used as possible while taking functions of the present invention into consideration, but this may vary according to the intention or precedent of a technician working in the field, the emergence of new technologies, and the like. In addition, in certain cases, there are terms arbitrarily selected by the applicant, and in this case, the meaning of the terms will be described in detail in the description of the corresponding invention. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall contents of the present invention, not a simple name of the term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise stated.

The present invention is one embodiment for solving the above problems, as a composition for skin protection that can protect the skin from various harmful environments by containing loquat tree (Eriobotrya japonica ( Thunb .) Lindl .) leaves or its active ingredients, It provides an aging liquid per mineral sugar of loquat tree leaves having an antioxidant effect, a protective effect against irritation, a soothing effect and an anti-pollution effect, and a method for preparing the same.

The present inventor devised an aging system that activates enzymes using minerals and sugars so that more potent ingredients contained in natural products can be produced by aging natural products. (MSA method). The aging method is more efficient than general solvent extraction by activating metabolism of natural products without any organic solvents or artificial manipulations, and it is possible to obtain safe raw materials having new effects and functions not found in existing natural products.

Since the natural product also has life, toxic substances are released to protect itself in the process of collecting. For this reason, in oriental medicine, medicines are not collected and used immediately, but are fermented through fungi, soaked in water for half a day, dried in sunlight, or evaporated in steam to dry. Will be used. In oriental medicine, this is called pojebeop. It is presumed that this was a way to lower bad energy and take good energy. The MSA method used in the present invention may also be approached in the same context, and summarizing this, it will mean that the MSA method not only increases the effect of natural products, but also contains alleviation of toxic substances. However, unlike the conventional fermentation performed by inoculating strains, the MSA method differs from the conventional fermentation method, since it is a method of aging in a sterile state using only sugars and minerals.

In the present invention, the loquat leaves take good energy through maturation, which is a kind of cloth making method, and by taking the phytoalexin emitted by the leaves of the Bifi tree in a crisis situation, the efficacy of the loquat leaves is increased, and new effects are created. It is something you can pay. By adding high sugar content and minerals to the loquat tree leaves, various nutrients inside are synthesized into phytoalexin, and by using this, human skin can have resistance to various harmful environments. On the other hand, loquat leaves contain a component called'amigdalin', which is a kind of cyanoglycoside and is a highly toxic substance capable of necrosis of a part of cells. However, in the present invention, by neutralizing the amygdalin component through aging using sugars and minerals, the bad energy of natural products is lowered, and positive effects such as antioxidant effect, protective effect against irritation, soothing effect and anti-pollution effect are highlighted. do. Therefore, the MSA method of the present invention can be said to be a kind of fabrication method that lowers bad energy and takes good energy.

In order to obtain the above raw materials, the aseptic ripening solution of loquat leaves having a soothing and regenerating effect using the minerals of the present invention is extracted and manufactured by mixing minerals and sugars with the loquat leaves, and aging them in a sterile state for a certain period of time. It is characterized by that. The minerals and sugars are mixed with the loquat leaves to improve the activity of enzymes, thereby increasing the yield of major physiologically active substances contained in the loquat leaves.

In all living organisms, various enzymes are contained as substances that act as catalysts for biochemical reactions. Since the enzyme is an organic catalyst made of protein, it exhibits all the properties of protein, and unlike inorganic catalysts, it is greatly affected by environmental factors such as temperature, pH (hydrogen ion concentration), and protein denaturing agents, and its three-dimensional structure is irreversibly denatured. If it does, it loses its activity. In other words, all enzymes act most actively under specific conditions, and one enzyme catalyzes one chemical reaction or a group of similar chemical reactions, does not cause rejection, and does not produce by-products.

Plants secrete phytoalexin due to their physiological activity in living organisms, which is when plants synthesize phytoalexin themselves as part of a defense mechanism when the plant is in an unfavorable environment. Plants can increase their own resistance to disease by producing antimicrobial substances called phytoalexins as an important defense against germs and repelling invaders and protecting themselves.

In the present invention, the method for preparing aseptic aging solution of loquat leaves having a soothing and regenerating effect using minerals does not add any organic solvent including water or heat in the process of obtaining the effective ingredients contained in the loquat leaves. It does not destroy specific enzymes that generate major physiologically active substances in loquat leaves by maximizing the activity without destroying specific enzymes that produce major physiologically active substances in loquat leaves based on the principle of osmotic pressure by only minerals and sugars. It can be obtained in liquid form.

The phytoalexin, in the present invention, is specifically synthesized by loquat leaves, and when the loquat leaves are placed under stress conditions, specifically, when placed in an osmotic pressure situation by minerals and sugars, loquat It is strengthened from the leaves of the tree. At this time, phytonutrient, a major physiologically active substance of loquat leaves, is fortified with phytoalexin. The phytonutrient is a physiologically active substance contained in vegetables and fruits, and has a function of inhibiting free radicals that attack cells in the body through strong antioxidant activity, and treating and preventing intracellular DNA damage.

The Loquat Tree ( Eriobotrya japonica ( Thunb .) Lindl . ) Is a plant of the genus Rosaceae Loquat, and is a small tall, evergreen 3 to 5m tall tree grown mainly for ornamental purposes. The yellow fruit is used for food. Leaves are called loquat leaves and seeds are called loquats, and when water is added to destroy them, the scent of benzaldehyde is produced.

In the present invention, a leaf of a loquat tree (Eriobotrya japonica ( Thunb .) Lindl . ) called loquat leaf (枇杷葉) is used.

In Korea, loquat trees are distributed in the southern coast of the Korean peninsula, Geoje Island, Wando, and other regions where there is no significant difference in temperature, and in the present invention, such loquat leaves or fruits, flower shoots, etc. are taken and ripened in a biological state.

The present inventors efficiently compress and extract the ripened loquat leaves obtained through the aging process without any extraction solvent including water, including minerals, sugars, and loquat leaves, and for the fermentation solution per mineral sugar of loquat leaves having skin protection effect. The effect of the extract was confirmed by measuring cellular activities such as antioxidant effect, protective effect against stimulation, sedative effect and anti-polluting effect.

The mineral includes various complex mineral components of any one or more of Ca, Mg, Cu, Zn, Na, K, Fe, P, Cl, Ge, Pt, Se, I, Ga, Bi, Te, Mo, Nb, In particular, it contains zinc, selenium, etc. in addition to magnesium, calcium, potassium and sodium, which are the four essential minerals for the human body, as well as a large amount of inorganic nutrients such as potassium and phosphorus, which are necessary for plant growth, to stabilize the leaves of loquat trees. It acts as a catalyst to promote the activity of various enzymes.

The mineral acts as a catalyst to allow more phytonutrient components, which are major physiologically active substances present in the leaves of the loquat tree, to be produced and eluted. Particularly, I, an element that can be included in complex minerals, is an element that has a beneficial effect that is beneficial to the human body, and it reacts with the phytonutrient component in the leaves of the loquat tree to expect an unpredictable effect. The content of the mineral is preferably 0.2 to 40% by weight, more preferably 1.0 to 30% by weight, and most preferably 5.0 to 15% by weight, based on the total weight of the mixture of saccharides and minerals.

The saccharides are sugar, honey, sucrose, glucose, allulose, tagatose, galactose, maltose, lactose, starch syrup, oligosaccharide, Any one or more of fructose may be selected and used, and preferably, any one or more of sugar, honey, sucrose, allulose, or oligosaccharide may be selected and used.

The saccharide of the present invention destroys the cell wall of the loquat tree leaf by osmotic pressure, so that phytonutrient components, which are the main physiologically active substances contained in the loquat tree leaf, are produced and eluted, as well as prevent contamination by putrefactive bacteria. It works to do. The mixing ratio of the saccharides and loquat leaves is preferably a weight ratio of 15: 85 to 65: 35, more preferably a weight ratio of 25: 75 to 50: 50, most preferably 30: 70 to 35: 65 weight ratio to be.

If the mixing ratio of the saccharides and loquat leaves is out of the weight ratio of 15:85 to 65:35, the content of saccharides is insignificant, so that the phytonutrient component of the loquat leaves is not easily produced and eluted, or by spoilage bacteria. It can be contaminated.

If the saccharide is more than 25% of moisture, the sterilization effect begins to appear, and accordingly, the effect of aging, not fermentation, can be obtained. If fermentation is performed because the ratio of sugar is low, it is determined that it is not the aging method using the mineral sugar of the present invention, which proceeds by aging, and thus there is a problem in that all the ingredients must be discarded. As described above, the present invention should proceed with aging, not fermentation.

When saccharides exceed 70% of moisture, the activity of enzymes present in loquat leaves may be suppressed by excessive saccharides, so the rate of production of phytonutrient components of loquat leaves decreases, resulting in skin protection effect. There is a problem in that the antioxidant effect, protective effect against irritation, soothing effect and anti-pollution effect of the fermented solution per mineral of loquat tree leaf decrease.

However, since the loquat leaves of the present invention are not composed of only moisture, and the total weight including other substances is measured, the mixing ratio of saccharides and loquat leaves is based on the weight ratio of 15:85 to 65:35. desirable.

In the present invention, in order to confirm the optimal aging conditions using minerals and saccharides, the aging tendency according to the content of the used minerals and the concentration of the loquat leaf extract was confirmed. Based on the aging period of 10 weeks, the content of minerals was tested by applying 0.01 to 70% by weight of the total weight of the mixture of sugars and minerals.

In the aging process, when the mineral content was 0.2 to 40% by weight, it was confirmed that the antioxidant effect, the protective effect against irritation, the sedative effect and the anti-pollution effect gradually increased. When the mineral content was 5.0 to 15% by weight, the most excellent effect was exhibited, and the effect decreased as the content of the mineral was closer to 0.01% by weight or 70% by weight out of 0.2 to 40% by weight. Therefore, it was confirmed that the mineral content based on the efficiency of using loquat leaves and saccharides is most appropriate to be mixed at 0.2 to 40% by weight based on the total weight of the mixture of saccharides and minerals.

If the content of the mineral is less than 0.2% by weight, the effect of enhancing the activity of the enzyme in the loquat leaves is insignificant due to the insignificant content of the mineral, and the effective phytonutrient component of the loquat leaves is produced and eluted lower. The antioxidant effect, protective effect against irritation, soothing effect, and anti-pollution effect of the fermented solution per mineral of loquat leaf, which has skin protection effect, are lowered.

If the content of the mineral is more than 40% by weight, the amount of minerals is excessive and the degree of generation and elution of the phytonutrient component of the loquat leaves due to the osmotic pressure of saccharides decreases, and the loquat leaves having a skin protective effect. There is a problem that the antioxidant effect, protective effect against irritation, sedative effect and anti-polluting effect of the aging liquid per mineral are somewhat reduced.

Hereinafter, a method of preparing a fermentation solution per mineral of loquat tree leaves having a skin protective effect of the present invention will be described in detail.

The method for preparing the fermentation solution per mineral sugar of loquat leaves having a skin protective effect of the present invention is

Washing and drying (draining) loquat leaves (S10);

In the step (S10) of the washed and drained loquat leaves of loquat trees (at optimum conditions) of saccharides and minerals are added, uniformly mixed, and then aged to prepare a fermented solution per mineral of loquat leaves having skin protection effect. (S20);

The step (S30) of producing a filtrate of ripening loquat leaves by compressing and filtering the fermented solution per mineral of loquat tree leaves having a skin protection effect of the step (S20) (S30); And

It includes a step (S40) of eluting the filtrate of ripening loquat leaves of the step (S30) for 1 to 2 weeks.

First, a step (S10) of washing the loquat leaves is performed. In the step (S10), after washing the loquat leaves with purified water to remove foreign substances, only water adhering to the surface of the loquat leaves is removed. At this time, it is preferable not to apply heat so that moisture contained in the loquat leaves is not lost.

Next, the step (S10) of mixing the washed and drained loquat leaves with saccharides and minerals (at optimum conditions) and then aging to prepare a fermented solution per mineral sugar of loquat leaves having a skin protection effect ( S20) is performed. The loquat leaves and saccharides of the step (S10) are mixed in a weight ratio of 35: 65 to 80: 20, preferably 50: 50 to 75: 25, more preferably 65: 35 to 70: 30 by weight, and at the same time The mineral responsible for the activity of the enzyme is mixed in an amount of 0.2 to 40% by weight, preferably 1.0 to 30% by weight, and more preferably 5.0 to 15% by weight, based on the total weight of the mixture of sugars and minerals. At this time, the step (S20) is preferably performed during the aging period of 12 to 24 weeks.

When the sugar content is more than 70% by weight, the dissolution effect may be somewhat easier, but this tends to lower the activity of the enzyme and thus the efficacy is lower than the optimal condition. On the other hand, in the case of less than 15% by weight, it is contaminated with microorganisms and fermentation or spoilage occurs.

If the ratio of minerals is less than 0.2% by weight of the applied saccharides, it has little effect on the activity of enzymes during the aging process, and if the ratio of minerals exceeds 40% by weight, the aging is not smooth, so it is effective and applied to cosmetic formulations. It has an adverse effect on not being smooth.

In the present invention, in order to check the optimal maturation period using minerals, sugars and loquat leaves, the content of minerals is applied in 0.2 to 40% by weight based on the total weight of the mixture of sugars and minerals, and the maturation status and skin protection on a weekly basis The tendency of the fermentation liquid per mineral of loquat tree leaf having an effect to promote collagen biosynthesis was confirmed. As a result of confirming the aging trend for a total of 48 weeks and about 1 year, after a aging period of about 12 to 15 weeks, the difference in efficacy did not increase significantly regardless of the more aging period. As the concentration increased, it could be confirmed that the antioxidant effect, the protective effect against stimulation, the sedative effect and the anti-pollution effect also increased.

On the other hand, if the aging period is less than 12 weeks, the aging period is too short, so the elution of enzymes and phytonutrient components from the aging solution per mineral of loquat tree leaves, which has skin protection effect, is small, resulting in antioxidant effect, protective effect against irritation, The sedative effect and anti-polluting effect were weak, and when the maturation period exceeded 24 weeks, the increase in antioxidant effect, protective effect against irritation, sedative effect and anti-pollution effect was slight, but rather decreased. That is, the time point at which the fermentation solution per mineral of the loquat tree leaf having the skin protective effect of the present invention reaches the optimum effect may be 12 to 24 weeks. Since there was no contamination by bacteria during the aging process, it was confirmed that there is no problem of deterioration of the aging solution per mineral of loquat leaves, which has skin-protective effects, even if it is aged for more than 24 weeks, but if it exceeds 24 weeks, enzymes and This is economically inefficient because the yield of the phytonutrient component is slowed.

When the aging period is 12 to 24 weeks, the antioxidant and wrinkle-improving effects of the aging liquid per mineral of loquat tree leaves having skin-protective effects are the most excellent. If the maturation period exceeds 24 weeks, the yield of the fermentation solution of loquat leaves does not increase significantly compared to the increase in the fermentation period. The degree to which the effect and anti-pollution effect is improved is insignificant.

In general, the aging liquid having a maturation period of 8 to 48 weeks (1 year) may be included in the scope of the present invention, and less than 8 weeks has an antioxidant effect, a protective effect against irritation, a sedative effect and an anti-pollution effect. It is difficult to use because it does not appear, and the aging period exceeding 48 weeks (1 year) is economically inefficient, and the antioxidant effect, protective effect against irritation, sedative effect and anti-polluting effect may rather decrease.

In summary, the aging period of the present invention is preferably 8 to 48 weeks, more preferably 12 to 24 weeks, and the most appropriate aging period is about 100 days (about 14 weeks).

The step (S20) is preferably performed at a temperature of 20 to 30°C, and when the temperature is 20 to 30°C, the enzymes and phytonutrient components of the loquat tree leaves are not altered or destroyed, and are produced and eluted. The antioxidant effect, protective effect against irritation, soothing effect, and anti-pollution effect of the fermented solution per mineral of loquat leaf, which has skin-protective effect, are the most excellent. If the temperature of the step (S20) is higher than 30°C, the enzyme begins to damage during maturation and decreases its activity, and the saccharides are deteriorated, so that the phytonutrient component of the loquat leaves is not sufficiently generated and eluted, and the skin Antioxidant effect, protective effect against irritation, soothing effect and anti-pollution effect of fermented solution per mineral of loquat leaf having a protective effect are inferior. If the temperature of the step (S20) is less than 20° C., the temperature is too low and aging does not proceed. Accordingly, there is a problem in that the enzyme and phytonutrient components of the loquat leaves are not properly eluted.

By separately taking only the liquid generated in the step (S20), the subsequent steps (S30, S40) may be performed. When only the liquid generated in the step (S20) is used in the subsequent steps (S30, S40), the enzyme and phytonutrient are liquefied only when the enzyme and phytonutrient components of the loquat tree leaf are matured. Since the purity of the phytonutrient component is increased, it can have more excellent efficacy in antioxidant effect, protective effect against irritation, soothing effect and anti-pollution effect.

The step (S30) of preparing a filtrate aged loquat leaves by filtering the fermented solution per mineral of loquat tree leaves having a skin protective effect in step (S20) is performed. The step (S30) may be performed using a pressure filter or a reduced pressure filter, and preferably may be performed using a reduced pressure filter.

After performing the step (S30), a step (S40) of eluting the filtrate of aging loquat leaves of the step (S30) for 1 to 2 weeks may be performed. In the step (S40), it is preferable to elute the filtrate of ripening loquat leaves with extraction solvents used in cosmetics of at least one of water, glycerin, butylene glycol, propanediol, and ethanol.

At this time, the step (S40) is preferably performed in a sealed container for 1 to 2 weeks. If eluted for less than one week in the step (S40), the antioxidant effect of the fermentation solution per mineral of loquat tree leaves, which has a skin protective effect, because many effective ingredients produced by the enzyme during the fermentation period are not properly extracted. , Soothing effect and anti-pollution effect are inferior to other times, and if aged for more than 2 weeks, compared to excessive elution, the antioxidant effect of aging liquid per mineral of loquat leaves, which has skin protection effect, protective effect against irritation, soothing It is economically inefficient due to the slight increase in the effect and anti-pollution effect.

The aging liquid per mineral of loquat leaves having a skin protective effect of the present invention can be applied to various fields, and in detail, may be included in a cosmetic composition.

The cosmetic composition can be prepared in various forms, and can be appropriately selected according to the purpose, and in detail, flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing It can be formulated as water, pack, spray, powder or lipstick.

In addition, the cosmetic composition may include other ingredients blended in a conventional cosmetic composition, if necessary. For example, it may contain a moisturizing agent, an organic or inorganic pigment, an ultraviolet absorber, an antioxidant, a pH adjuster, an alcohol, a colorant, a fragrance, and the like.

In addition, the aging solution per mineral of loquat leaves having a skin protective effect of the present invention may be included in a pharmaceutical composition or a composition for external application.

Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples and Experimental Examples. These Examples, Comparative Examples and Experimental Examples are for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1. Fermented solution per mineral of loquat leaf that has skin protection effect ( Bio - Liqu ) Of the manufacture

Using the following manufacturing method, a fermentation solution (Bio-Liqu) per mineral of loquat leaves having a skin protective effect was prepared.

S10: After washing the loquat leaves with purified water to remove foreign substances, only the moisture adhering to the surface of the loquat leaves was removed.

S20: The loquat leaves and sugar of the step S10 are mixed in a weight ratio of 3: 1, and the complex minerals are mixed at 10% by weight based on the total weight of the mixture of sugar and minerals, and then aged for 24 weeks at room temperature of 20 to 30°C. Thus, an aging solution per mineral of loquat leaves having a skin protective effect was prepared.

S30: The loquat leaves of the step S20 were filtered with a vacuum filter to prepare a filtrate for ripening loquat leaves.

S40: Fermented solution per mineral of loquat tree leaves having skin protection effect by eluting the fermented solution per mineral of loquat tree leaf having skin protection effect of step (S30) with 30% butylene glycol (remaining water) for 1 week (Bio-Liqu) was prepared.

Comparative example 1. General Loquat Leaf Extract ( Liqu . ext ) Of the manufacture

Using the following manufacturing method, a loquat leaf extract (Liqu.ext) was prepared.

After washing the loquat leaves with purified water to remove foreign substances, only the moisture adhering to the surface of the loquat leaves was removed, and then 30% EtOH was used as an extraction solvent and extracted with a vacuum filter to extract a general loquat leaf extract (Liqu.ext). Was prepared.

Experimental example One. Free radical Erasing ability Experiment

Through the free radical scavenging effect using the DPPH method, the antioxidant effects of the fermentation solution (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat tree leaf having skin protection effect were confirmed.

Using purified water, increase the sample by 2 times based on 100% of the volume of the aging solution that has been aged, and then dilute and dilute the purified water to the sample at 0 times, 2 times, 4 times, 8 times, 16 times, 32 2,2-diphenyl-1-picrylhydrazyl (DPPH) solution dissolved in ethanol to 1 mM after making a sample by diluting it in times, 64 times, 128 times, 256 times, and 512 times, and dispenses 100µl each into 96 microplates. Was added 10 μl. After 30 minutes reaction at room temperature, absorbance was measured at 517 nm to measure free radical scavenging activity. In order to evaluate the degree of antioxidant effect of the samples with 0.1% vitamin C as a stock solution, they were placed as a control group and tested together.

Magnification at which the number of correct numbers is diluted in the sample (volume% of the sample) (Control)
Free radical scavenging effect of vitamin C (0.1%) (%) (Example 1)
Free radical scavenging effect of bio-Liqu per mineral of loquat leaves with skin protection effect (%) (Example 2)
Free radical scavenging effect of loquat leaf extract (Liqu.ext) (%) 0 (100%) 100% 100% 100% 2 (50%) 100% 100% 100% 4 (25%) 100% 100% 100% 8 (12.5%) 100% 100% 100% 16 (6.25%) 90.7% 100% 100% 32 (3.125%) 78.2% 100% 97.2% 64 (1.5625%) 41.3% 96.6% 77.4% 128 (0.78125%) 36.2% 76.4% 62.5% 256 (0.390625%) 25.7% 59.8% 41.2% 512 (0.1952125%) 20.8% 42.5% 30.4%

As a result, it was confirmed that the free radical scavenging effect was observed in the fermentation liquid (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat leaf, which has skin protection effect, and is higher than that of vitamin C, the control group. It was confirmed that it has an antioxidant effect. Overall, it was confirmed that the present invention, the fermentation solution per mineral of loquat tree leaf having skin protection effect (Bio-Liqu) exhibited a higher antioxidant effect than that of the general loquat tree leaf extract (Liqu.ext).

Experimental example 2. Cell stability test

A cytotoxicity test was performed for HaCaT, which is a human keratinocyte. To evaluate cytotoxicity, Neutral red uptake assay was used.

Loquat leaf mineral sugar fermentation solution (Bio-Liqu) and general loquat leaf extract (Liqu.ext), which have skin-protective effects using minerals on HaCaT, human keratinocytes, were 1.0%, 0.5%, 0.25%, respectively. % And 0.12% concentration was added, and the cell viability was measured using the NRU test.

The result of Experimental Example 1 is that the value before the addition of the fermentation liquid (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat tree leaves having skin protection effect using minerals is set to 100%, and HaCaT Cell viability was expressed as a percentage.

Sample input concentration Cell viability (%) of fermented liquid (Bio-Liqu) per mineral of loquat leaf having skin protection effect Cell viability of loquat leaf extract (Liqu.ext) (5) 1.0% 91.5 71.6 0.5% 95.2 82.7 0.25% 97.2 92.5 0.125% 100.0 100.0 0.0625% 97.2 97.1 0.03125% 99.7 99.3

As a result, it can be confirmed that both the fermentation liquid (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat leaves having skin protection effects have cell stability at low concentrations, but the higher the concentration, the more general It was confirmed that the cell stability of the loquat leaf extract (Liqu.ext) was weakened, while the aging liquid per mineral of the loquat leaf (Bio-Liqu), which has a skin protective effect, maintained cell stability even when the concentration was increased. From this, it was confirmed that the fermentation solution (Bio-Liqu) per mineral sugar of loquat leaves, which has skin protection effect, is a safe raw material without cytotoxicity.

Experimental example 3. Cell protection effect test from stimulus

In order to confirm the cytoprotective effect of Bio-Liqu per mineral of loquat leaf, which has skin-protective effect, how much it can protect the cells from the irritants of each sample when the cells are stimulated. Confirmed.

To confirm the effect on cell growth, primary cultured human dermal fibroblast (HDFN) was used. HDFN cells subcultured with FGM-2 (LONZA) medium were trypsin treated and recovered by centrifugation, and then the number of cells was adjusted using the test medium, and the effect on cell growth was measured. ^{Specifically, after adjusting HDFN to 2.0 × 10 4} cells/mL in DMEM medium containing 0.2% FBS and 0.1% BSA, 135 µl of this cell suspension was dispensed into a 96 microplate, and then overnight in an incubator. After pre-culture and replacement with the same media, samples diluted at each concentration were added, cultured for 3 days, and UV irradiated for 40 seconds to confirm the cell growth state after stimulating cell growth. Cell growth was measured using a neutral red uptake assay.

Sample concentration in which the sample is diluted in purified water (% by volume) (Example 1)
Cell survival rate (%) of the test group in which the fermentation solution (Bio-Liqu) per mineral of loquat leaves having skin protection effect was added (Comparative Example 1)
Cell survival rate (%) of the test group into which the loquat leaf extract (Liqu.ext) was added 1.0 59.69 31.57 0.5 54.39 26.55 0.25 43.90 23.85 0.125 39.19 20.41 0.0625 20.70 19.74 0.3125 20.90 19.15

Experimental example 4. Soothing effect experiment

A simplified clinical test was conducted on the skin irritation relief and soothing effects of the bio-liquor (Bio-Liqu) per mineral of loquat leaf having skin protection effect.

Skin irritation was applied to healthy adult men and women aged 20 to 40 years using the tape scraping method. In order to reduce the variable, 6 areas were selected on one wrist, and the tape was attached to the same area 13 to 15 times and removed repeatedly to damage the skin, causing pain and irritation.

Then, the solubilization formulation containing 2% of the aging liquid per mineral of loquat tree leaves (Bio-Liqu) having skin-protective effect prepared in Example 1 and the aging liquid per mineral of loquat tree leaf having skin-protecting effect (Bio-Liqu ) Was applied to the bean cream formulation that did not contain, and the trend was confirmed for 3 days. The degree to which the skin became irritated and turned red was evaluated visually.

As a result, as shown in Figs. 4 to 5, the solubilizing formulation containing 2% of the aging liquid (Bio-Liqu) per mineral of loquat leaves having a skin protective effect showed a soothing and improving effect in a day after use. It was confirmed that the wound was recovered after ~ 7 days. On the other hand, the bean cream formulation that did not contain the bio-liqu per mineral per loquat leaf, which has a skin protective effect, showed a tendency to worsen the wound.

Experimental example 5. Anti-contamination Effect experiment

An anti-contamination effect experiment was conducted on the fermentation solution (Bio-Liqu) per mineral of loquat tree leaf having skin protective effect of Example 1 and the general loquat leaf extract (Liqu.ext) of Comparative Example 1.

Skin keratinocytes were used to measure the degree of cell damage caused by pollutants, and the fermentation solution per mineral of loquat leaf (Bio-Liqu) and the general loquat leaf extract of Comparative Example 1 (Liqu.ext) having a skin protective effect were used. Treatment by concentration was performed and the effect of inhibiting skin cell damage was confirmed. Human keratinocyte HaCaT was dispensed into a 96-well plate at a concentration of 1×10⁴cells/ml and incubated for 24 hours. The fermentation liquid per loquat leaf mineral (Bio-Liqu) and the general loquat leaf extract (Liqu.ext) of Comparative Example 1 were treated and further cultured for 24 hours. After that, 5mg/mL MTT solution was added and incubated for 3 hours, and then the medium was removed, 150 µl of dimethyl sulfoxide (DMSO) was added to each well, shaking for 10 minutes, and absorbance was measured under the condition of 570 nm (reference 655 nm). The experimental group that did not treat any of the fermentation solution (Bio-Liqu) and general loquat leaf extract (Liqu.ext) per mineral of loquat leaves having skin protection effect was used as a control group. The survival rate of the control group was at 2.5 ㎍/mL of contaminants. Refers to the survival rate of In this experimental example, the survival rate of skin keratinocytes not treated with contaminants was expressed as a percentage based on 100%.

Cell viability of untreated group of contaminants
(%) Control cell viability
(%)
density
(μg/mL) Cell viability (%) of fermented liquid (Bio-Liqu) per mineral of loquat leaf having skin protection effect Improving cell viability of bio-Liqu per mineral of loquat leaves with skin protection effect (%) Cell viability (%) of loquat leaf extract (Liqu.ext) Improvement of cell viability of loquat leaf extract (Liqu.ext) (%)

100

57.6 5.0 67.7 10.1 57.7 0.1 10.0 73.8 16.2 57.8 0.2 15.0 78.4 20.8 59.2 1.6 20.0 87.7 30.1 60.7 3.1 25.0 90.3 32.7 61.2 3.6

The viability improvement was calculated as the difference between the cell viability of the control group and the viability of each sample treatment group.

As a result, it was confirmed that the cell viability of the loquat leaf extract (Liqu.ext) did not increase significantly compared to the control group, but the fermentation solution per mineral of loquat leaves of Loquat (Bio-Liqu), which has a skin protective effect, significantly increased the cell viability. And it was confirmed that the cell viability increased according to the concentration.

In other words, if benzopyrene, ammonium nitrate, formaldehyde concentration 2.5 μg/mL, and the ripening liquid (Bio-Liqu) per mineral of loquat tree leaf having skin protection effect according to the manufacturing method of the present invention is applied at 5.0 to 25.0 μg/mL It was confirmed that the cell viability was improved by 10.1 to 32.7% based on the difference value compared to the experimental group that was not treated with anything.

Therefore, it can be confirmed that the aging liquid (Bio-Liqu) per mineral sugar of loquat leaves having skin protection effect according to the manufacturing method of the present invention has anti-pollution ability against air pollutants including benzopyrene, ammonium nitrate, and formaldehyde. there was.

Although the present invention has been described with the accompanying drawings, this is only one of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. As for the purpose, it is clear that the present invention is not limited to the above-described embodiments. Therefore, the scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are subject to the rights of the present invention Will be included. In addition, it is clarified that some of the configurations in the drawings are provided to be exaggerated or reduced than in actuality, as for describing the configuration more clearly.

Claims (5)

Washing and drying loquat leaves (S10);
Among the saccharides and Ca, Mg, Cu, Zn, Na, K, Fe, P, Cl, Ge, Pt, Se, Bi, Te, Mo, Nb on the leaves of the Loquat tree washed and dried in the step (S10) A step of preparing an aged solution per mineral of loquat leaves having a skin protective effect that strengthens the phytonutrients, the main physiologically active substances of loquat leaves, with phytoalexin by adding any one or more minerals, mixing them uniformly, and aging them ( S20);
The step (S30) of producing a filtrate of ripening loquat leaves by compressing and filtering the fermented solution per mineral of loquat tree leaves having a skin protection effect of the step (S20) (S30); And
Antioxidant effect, skin protection effect, skin soothing effect and air pollution including benzopyrene, ammonium nitrate, formaldehyde including the step (S40) of eluting the filtrate of the loquat leaves of the step (S30) for 1 to 2 weeks Method for producing a fermented solution per mineral of loquat tree leaves having skin protective effect, characterized in that it has an anti-polluting effect on the substance.
delete The method of claim 1,
The saccharide is any of sucrose, glucose, allulose, tagatose, maltose, lactose, honey, starch syrup, oligosaccharide, and fructose. Method for producing a fermented solution per mineral sugar of loquat tree leaves having a skin protective effect, characterized in that it contains one or more saccharides.
The method of claim 1,
The mineral is a method for producing a fermented solution per mineral of loquat tree leaves having a skin protective effect, characterized in that the mineral is mixed in 0.2 to 40% by weight based on the total weight of the mixture of saccharides and minerals.
Claims 1, 3, 4 It is prepared by the method of any one selected from among the antioxidant, skin protection effect, skin soothing effect and anti-pollution effect, characterized in that it has a skin protection effect of a loquat tree leaf mineral sugar ripening solution.

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