KR20210002875A - Cosmetic composition for anti-inflametion, anti-wrinkle, whitening and anti-bacterial comprising Extract of Chrysanthemum morifolium - Google Patents

Abstract

The present invention relates to an organic solvent extract from Chrysanthemum morifolium RAMAT. of Udo island in Jeju and to an anti-inflammatory, anti-wrinkle, whitening and anti-bacterial functional cosmetic composition comprising the same as an active ingredient.

Description

Cosmetic composition for anti-inflametion, anti-wrinkle, whitening and anti-bacterial comprising Extract of Chrysanthemum morifolium}

The present invention relates to a skin anti-inflammatory, anti-wrinkle and antibacterial functional cosmetic composition containing Jeju Udo chrysanthemum flower extract as an active ingredient.

Until now, a number of studies on the antioxidant activity, whitening, and anti-inflammatory activity of Asteraceae plants have been known, but no studies have been conducted on the anti-inflammatory, anti-wrinkle, whitening and antibacterial activities of the chrysanthemum flowers in Jeju Udo.

Chrysanthemum ( Chrysanthemum morifolium RAMAT.) is a perennial plant belonging to the Chrysanthemum family Asteraceae. It is known to be native to China, and is widely grown in mountainous areas in the south central region of Korea. Other names for chrysanthemum are Gamgukhwa (甘菊花), Jeolhwa (節華), Iljeong (日精), Jooyoung (周盈), Buyeonyeon (傅延年), Yeojeol (女節), Yeokyung (女莖), and rehabilitation (更生). )·Eumseong(陰城).

In addition, chrysanthemum has a flat nature, has a sweet taste, and has no poison, so it comforts the long-term, improves the five veins, makes limbs well, and is used for dizziness and headaches. In addition, it is known as a medicinal herb that helps blood congestion in the eyes, stops tears, cools the head and eyes, and treats wind and rain.

In particular, the antipyretic action, blood pressure lowering action, and cardiovascular dilatation action were found in experiments.

In chrysanthemum, ingredients such as essential oils, flavonoids, sesquiterpenoids, and triterpenoids are known.

Chrysanthemum water extract and flavonoids are known for their distinct antioxidant activity. It is known that it can enhance the removal of free radicals in human cells and inhibit aging.

Skin aging is largely divided into two studies, one of which is'internal aging', which is an aging phenomenon with increasing age, and the other is'external aging', which refers to an aging phenomenon caused by external environment, such as ultraviolet rays or pollution. . There have been several theories on skin aging so far, but among them, the most scientifically approached theory for skin aging is the skin aging theory due to oxidation.

Skin aging due to oxidation is due to free radicals, and the free radical theory was proposed by D. Harman in the mid-1950s. Free radicals are produced by the phagocytosis of leukocytes, the electron transport system in the process of ATP production in mitochondria, the action of myeloper oxide (MPO), ultraviolet rays, cigarettes, normal metabolic processes, stress, pollutants, and bacteria. When radicals remain in the human body due to a cause, various problems such as wrinkle formation, skin cancer, cell killing, rheumatoid arthritis, atopic dermatitis, acne, etc. can be solved through destruction of cells, cutting of connective tissue, and induction of cross-linking. Occurs. In the human body, natural antioxidants (radical scavengers) such as SuperOxide Dismatase (SOD), catalase, vitamin E, vitamin C, and ubiquinol are present and can remove free radicals. The antioxidant system gradually begins to decline due to age, pollution, ultraviolet rays, and stress, and the antioxidant system becomes incapacitated, which in turn leads to an increase in free radicals in the body. Increased radicals destroy the connective tissues of the dermis, such as collagen, elastin, hyaluronic acid, etc., causing skin subsidence (wrinkles), and oxidizing the lipid part of the cell membrane to destroy cells, resulting in dermatitis and acne. , Cause skin cancer and other diseases. In addition, this radical is involved in the spontaneous oxidation reaction during the melanin formation process, causing spots, freckles, and wrinkles (Journal of the Korean Cosmetics Association, Volume 23, No. 1, 75-132).

Allergy is a phenomenon that causes an antigen-antibody reaction when an antibody that reacts to the antigen is generated when an antigen is first introduced into the body, and then the same substance enters the body again. Symptoms such as hypersensitivity, sneezing, and hives appear. . The antigen does not cause any problems in the general population, but it causes hypersensitivity in a specific person, and when allergen, a substance causing allergy, enters the body with the antigen, an antibody (IgE) against the allergen is produced. When the allergen is attached to the mast cell in the tissue and the causative allergen re-invades, it binds to the IgE of the mast cell and degranulates the mast cell, resulting in histamine, serotonin, and renin. ), etc., are released, causing sneezing and itching.

Allergy-related diseases due to the recent increase in environmental pollutants, changes in diet, and various stresses are on the rise, and it is reported that more than 5% of the total population are allergic disease patients in the 2000s. In addition, allergy, which is a biochemical phenomenon that exhibits a specific transformation reaction to foreign substances (Allergen or Antigen), is caused by degranulation that occurs in mast cells, etc. Degranulation is caused by binding of antigens to Fc receptors, anti-IgE, lectins, etc. and increasing calcium. Caused by Recently, the cause of allergic diseases has been gradually identified, but drugs that can cure allergic diseases have not yet been developed, but only the effect of improving symptoms is expected.

As a result of the present inventors' diligent efforts to find a more effective composition in a situation where there is no special treatment method for oxidation or allergic inflammatory reactions, Jeju Udo chrysanthemum flower extract is useful for preventing or treating antioxidant and inflammatory diseases. On the other hand, it was found that the whitening and antibacterial functional cosmetic composition has excellent effects, and the present invention was completed by developing an ultrasonic extraction method as a method for effectively extracting it.

Korean Patent Publication No. 10-278203 Korean Patent Application Publication No. 10-2009-0042450 Republic of Korea Patent Publication No. 10-2017-0100076 Korean Patent Application Publication No. 10-2006-0108864

EL-Domyati M et.al., Exp Dermatololl, 2002 Serafin, WE, In Goodman and Gillmans, The Pharmacological Basis of Therapeutics, Hardmen, Ja; Limbird, L,E eds, Mc Graw-Hill, Newyork, 1996, 659-682 Townley RG & 0kada, C, Annals of Allergy, 68, 1991, 190-196 korean J. plant Res., 23(1):47, 2010

Accordingly, an object of the present invention is to provide a Jeju Udo chrysanthemum flower extract and a cosmetic composition containing the same as an active ingredient.

Another object of the present invention is to provide an anti-inflammatory, anti-wrinkle, whitening and antibacterial functional cosmetic composition for skin using the method for preparing the Jeju Udo chrysanthemum flower extract and the chrysanthemum flower extract prepared according to the method as an active ingredient.

The object of the present invention is to prepare an extract of Jeju Udo chrysanthemum flower (Chrysanthemum Morifolium); It consists of filtering the Jeju Udo chrysanthemum flower extract obtained in the above step using a membrane filter, and by evaluating the anti-inflammatory, anti-wrinkle, whitening and antibacterial activity of the Jeju Udo chrysanthemum flower extract obtained in the above step. Achieved.

According to the present invention, there is an effect of providing a new Jeju Udo chrysanthemum flower extract, and a novel cosmetic composition with excellent anti-inflammatory, anti-wrinkle, whitening and antibacterial activity containing it as an active ingredient, and excellent effect of providing cosmetics of various formulations. have.

1 is a graph showing the experimental results of the anti-inflammatory effect of the Jeju Udo chrysanthemum flower extract according to the present invention.
2 is a graph showing the experimental results of the anti-wrinkle effect of the Jeju Udo chrysanthemum flower extract according to the present invention.
3 is a graph showing the experimental results of the whitening effect of the Jeju Udo chrysanthemum flower extract according to the present invention.
4 is a graph showing the experimental results of the antioxidant effect of the Jeju Udo chrysanthemum flower extract according to the present invention.
5 is a photographic view of a chrysanthemum flower plant in Jeju Udo used as a test material according to the present invention.

Hereinafter, the present invention will be described in detail according to embodiments of the present invention. The following examples only illustrate the present invention, but the scope of the present invention is not limited to the following examples.

Preparation of the present invention Jeju Udo chrysanthemum flower extract

After adding 40 to 60 mL of 40% butylene glycol to 4 to 5 g of dried Jeju Udo chrysanthemum flowers, extraction was performed after fixing the temperature at 40°C using a solificator (JAC 4020, Jinwoo, Korea). The sample thus extracted was filtered through a 0.45 μm membrane filter to obtain the Jeju Udo chrysanthemum flower extract of the present invention. All processes were repeated 3 times for 1 hour.

After obtaining the Jeju Udo chrysanthemum flower extract prepared by the above method, it was refrigerated at 40°C and provided for the experiment.

The yield of Jeju Udo chrysanthemum flower extract according to the type of solvent and ultrasonic treatment conditions used in the solvent extraction process according to the present invention is as follows (Table 1).

division Solvent used Yield (g)
(0.45㎛ membrane filter filtration) Solificator ultrasonic key (40.5KHz) Example 1 70%
1,3-butylene glycol 125.4 Initial 250w (40℃)/
Late 500w(40℃)/1hr Comparative Example 1 water 84.3 Homology Comparative Example 2 30% ethanol 96.7 Homology Comparative example 70% ethanol 101.1 Homology

In the above treatment solvent and conditions, an ideal extract without suspension could be obtained.

Functional efficacy test of Jeju Udo chrysanthemum flower extract

Experimental Example 2-1. Anti-inflammatory effect of the present invention Jeju Udo chrysanthemum flower extract

The mouse macrophage cells, RAW 264.7 cells, were artificially treated with LPS (Lipopolysaccharide), a substance that induces inflammation, and then we evaluated whether they had an inhibitory effect on inflammation. After dispensing to a 96 well plate to a size of 2×10 ⁵ cells per well, it was incubated in a 37°C, 5% CO2 incubator for 24 hours. After overnight and exchange with a new medium, LPS (1 ug/mL) and extract were administered by concentration and cultured for 24 hours. After incubation, the supernatant of the medium was taken and centrifuged at 13,000 rpm for 3 minutes, only the supernatant was collected, reacted 1:1 with Griess reagent, and absorbance was measured at 570 nm.

As a result of the experiment, Jeju Udo chrysanthemum flower extract effectively inhibited NO generation depending on the concentration, and showed excellent NO inhibition ability of 69.1% when treated with 2% (Fig. 1).

Experimental Example 2-2. Anti-wrinkle test of the present invention Jeju Udo chrysanthemum flower extract

Skin fibroblasts (HDFn) were inoculated with 0.3 mL of culture medium at a concentration of 5×10 ⁴ cells/well in a 48 well plate and cultured at 37°C and 5% CO2. After 24 hours, the extract was cultured for 24 hours in a new medium containing each concentration. After culturing for 24 hours, the culture medium was collected and the amount of the C-terminus of the collagen precursor was measured using an enzymes-linked immunoassay kit (Takara). In addition, a standard curve between 0 ng-640 ng of collagen concentration was obtained using 640 ng/mL of collagen solution, and the amount of collagen in the medium was calculated.

As a result of the experiment, Jeju Udo chrysanthemum flower extract showed an effect of increasing collagen synthesis depending on the concentration, and when 2% was added, it showed 23% collagen synthesis ability (FIG. 2).

Experimental Example 2-3. Antibacterial Effects of the Invention Jeju Udo Chrysanthemum Flower Extract

[Experiment method]

1. Test strain

Staphylococcus aureus: After incubating for 24 hours at 37℃ on an LB ager plate, a single colony was taken, inoculated in LB liquid medium, and incubated for 18 hours in a shaking incubator at 30℃. (Number of bacteria around 10 ⁹ cfu/ml)

Escherichia coli: After incubating for 24 hours at 37°C on an LB agar plate, a single colony was taken, inoculated in LB liquid medium, and incubated for 18 hours in a shaking incubator at 30°C. (Number of bacteria around 10 ⁹ cfu/ml)

Candida albicans: After incubation at 30℃ for 24 hours on a YM agar plate, a single colony was taken, inoculated in YM liquid medium, and incubated for 18 hours in a 30℃ shaking incubator. (Number of bacteria around 10 ⁹ cfu/ml)

2. Concentration of 100%, 50%, 25%, 12.5%, 6.2% of Jeju Udo chrysanthemum flower extract and positive control Tetracycline (50μg/ml, 12.5 μg/ml), Ketoconazole 0.25% and each strain as much as 104 After putting it in the well, it was incubated at 30°C for 20 hours in a shaking incubator and measured at absorbance 600, and the measured value was converted into inhibitory activity.

As a result of the experiment, E. coli and C. albicans showed antibacterial activity in a concentration-dependent manner (Table 2).

2-4. Whitening effect experiment of the present invention Jeju Udo chrysanthemum flower extract

The murine melanoma (B16 F1) cells were inoculated with an appropriate amount of cells per well in a 24 well plate with DMEM medium containing an appropriate amount of FBS (fetal bovine serum), and cultured at 37°C for 24 hours at 5% CO2. The medium of the culture medium was removed, the sample was replaced with a medium diluted to an appropriate concentration, and cultured again at 37°C for 72 hours at 5% CO2. After cultivation, the culture medium was removed, and the cell pelle obtained above was dried at 60° C., and then 1 M sodium hydroxide solution containing 10% DMSO was added to obtain intracellular melanin in a 60° C. thermostat. The absorbance of the reaction solution containing melanin obtained above was measured at 405 nm with a microplate reader to measure the amount of melanin per cell number or the amount of melanin per certain protein.

As a result of the experiment, when 2% of Jeju Udo chrysanthemum flower extract was treated, the effect of inhibiting melanin production by 21.7% was confirmed (FIG. 3).

2-5. Antioxidant effect experiment of the present invention Jeju Udo chrysanthemum flower extract

Skin aging processes by free radicals include lipid peroxidation, protein oxidation, DNA oxidation, melanogenesis, cleavage of connective tissue and abnormal crosslinking. Most of these reactions are radical reactions and include automatic oxidation processes. Antioxidants are responsible for blocking the automatic oxidation reaction in vivo. The greater the ability to give electrons, that is, the reducing power, the stronger the antioxidant. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a reagent that can measure the reducing power of antioxidants. The maximum absorption wavelength of DPPH is 517 nm, and the radicals are scavenged by aromatic compounds and amines as they contain radicals in the molecule, resulting in decolorization of deep purple. Add 100 μL of sample solution of various concentrations to 100 μL of 0.2 mM DPPH methanol solution, mix, react at room temperature for 20 minutes, and measure the degree of decrease in absorbance at 517 nm. The magnitude of the reducing power of the sample is expressed as the radical scavenging concentration (SC50), and SC ₅₀ is the concentration of the sample required to reduce the DPPH concentration by 50%. Ascorbic acid was used as a positive control.

As a result of the experiment, the Jeju Udo chrysanthemum flower extract showed an SC ₅₀ (50% Scavenging concentration) value at 1.3% (v/v), and the antioxidant efficacy was confirmed according to a dose-dependent increase (FIG. 4).

Preparation of a cosmetic formulation containing the Jeju Udo chrysanthemum flower extract as an active ingredient of the present invention Hereinafter, an example of a cosmetic formulation containing the Jeju Udo chrysanthemum flower extract according to the present invention will be described, but the present invention is not limited thereto.

3-1. Anti-inflammatory essence manufacturing (% by weight)

3-2. Anti-wrinkle cream (wt%)

3-3. Antibacterial cleansing (% by weight)

Claims (3)

Jeju Udo Chrysanthemum flowers made by adding 5 to 15 times 70% 1,3-butylene glycol to the dried Jeju Udo Chrysanthemum (Chrysanthemum Morifolium) and extracting for 1 hour at 250 to 500w 40℃ in the early and late stages with a 40.5KHz ultrasonicator extract Anti-inflammatory, anti-wrinkle, whitening and antibacterial functional cosmetic composition containing the Jeju Udo chrysanthemum flower extract of claim 1 as an active ingredient The cosmetic composition of claim 2, wherein the cosmetic composition is any one formulation selected from an essensor, cream, lotion, cleansing, and paste.

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