KR20160069170A

KR20160069170A - Method of propagating Chamaecyparis pisifera by cutting

Info

Publication number: KR20160069170A
Application number: KR1020140174771A
Authority: KR
Inventors: 김장수; 강영제; 나성준
Original assignee: 대한민국(산림청 국립산림과학원장)
Priority date: 2014-12-08
Filing date: 2014-12-08
Publication date: 2016-06-16

Abstract

The present invention relates to a method for growing a flowerpot, which can shorten the production period of seedlings while maintaining the same qualities of genetically same, by using a cutting method which is a silent propagation method, and can mass-produce the seedlings at low cost.

Description

[0001] The present invention relates to a propagation method of propagating Chamaecyparis pisifera by cutting,

The present invention relates to a plant propagation method using a cutting, and by using cutting as a silent propagation method, it is possible to shorten the production period of the planting while maintaining the same qualities of genetically same parameters, The present invention relates to a method of propagating a flower.

Painter ( Chamaecyparis pisifera (S. et Z.) Endl.) is an evergreen needlework tree that grows to a depth of 30 ~ 40m and a diameter of 80 ~ 100cm. It has a pointed end of leaf and a white pore-forming line on the back is W-shaped.

The flowerbags introduced from Japan in 1924 were mainly planted in the southern part of the central region at a depth of less than 500m. The flowers are quicker to grow and have better sound resistance, weather resistance and cold resistance than those of the same genus. It is wide.

Wood is used as building materials, civil engineering, furniture, and machinery materials. Especially, it is strong in moisture and moisture, and it is popular in Japan as a building material for bathroom. In addition, it is cultivated in the downtown area because it is strong in the environment, and it is cultivated as environment water, scenic waterpark, scenic waterpark, etc., and is used as evergreen sanctuary.

In recent years, as interest in natural materials of wood has increased, studies on the extraction of useful components have been conducted. Mono-terpenoids (α-pinene, δ-3-carene, myrcene, terpinolene, -α-menthen-8-yl-acetate) is known to be more abundant than that of untwisted trees (Hong-Chun Hong, Kim Chul-sun, Kim Nam-gyun, Kim Young-hee 2001. Composition of essential oils isolated from leaves and fruits Applied Biochemical Society 40 (2): 116-121).

Artificial reproduction of flowers is usually done by seeding and storing and then sowing in spring. When it is young, it shows negative water, so it must be sunk after sowing.

Shrimp breeding is a method of silent breeding in which a part of a plant is cut and inserted into sand or soil to reproduce a new individual. It is a method to obtain a large quantity of the same traits in a short period of time. the propagation method used (Hartmann, HT, DE Kester, FT Davies, JR, RL Geneve 2011. HARTMANN &KESTER'S Plant propagation:...... Principles and practice (8th ed) Prentice-Hall, USA, pp 280 -343).

In addition, it is more economical than grafting, and there are no problems such as the influence of rootstocks and the incompatibility of grafting, and so much research has been conducted on the production of seedlings by cutting.

Many studies have been carried out to increase rooting rate at home and abroad. The rooting rate is known to be closely related with the sampling time of the cutting, the age of the shoot, the concentration and type of rooting accelerator, clone, cutting area, ( Kim Tae Soo, Kim Chan-soo, 1988. Effect of seedling harvesting, cutting time, and plant growth factors on the rooting of coniferous tree trunks.) 24: 47-56; ( Larix ( Larix ), Larix ( Larix ), Larix ( Larix ), and Larix Leptolepis ) on Rooting Rooting. Korean Journal of Microbiology 83 (2): 205-210; Song, Jeong - Joo, Jeong - Ju Lee, Young - Bong Lee, Byoung - Yeon Lee, Sang - Don Han, 2006. Growth of shoots of rare plants ( Thuja koraiensis Nak.). Korean Journal of Microbiology 95 (4): 393-397; Kim, CS and ZS Kim. 2007. Effects of auxin and fog treatments on the green-wood cutting of the mature trees in Prunus yedoensis J. Kor. For. Soc. 96 (6): 676-683).

However, there has not yet been studied the growth of the planting of the flower, and research and development thereof is urgently required.

The inventors of the present invention conducted continuous research to solve the problems of the prior art as described above. The inventors of the present invention have found that the environment suitable for cutting at the time of cutting, the length of the cutting, the cutting time, Concentration, the cultivated soil of the cuttings, the seedlings of the seedlings, and the like. The present invention has been completed based on this finding.

Accordingly, it is an object of the present invention to provide a method for growing a flowerpot using a cutting method for mass proliferation of a flower bud.

Another object of the present invention is to provide a flower pot grown by the above method.

(A) treating the shoots collected from the flower buds with rooting accelerators, (b) cutting the roots treated with the rooting accelerator to a culture soil, and (c) ) Growing the seedlings that have been roasted after the above-mentioned cutting, and a method of propagating the plant using the cutting.

Further, the present invention provides a flower pot grown by the above method.

By using the cutting method as a silk propagation method according to the present invention, it is possible to maintain the same qualities of genetically same in a flower color as it is, and it is possible to shorten the production period of a seedling in the spread of a good variety developed through breeding And can be mass-produced at low cost.

Fig. 1 shows changes in rooting rate (A), number of rooted root (B) and root length (C) after cutting of a flower bud.
Fig. 2 is a photograph showing an artificial soil mixed with peatmoss: pearlite: vermiculite at a volume ratio of 1: 2: 2, and a flower pot cut in a clay (volcanic stone).
FIG. 3 shows the roots development characteristics of the cutting according to cutting time, cutting length, and rooting agent treatment conditions in the flower bud mature tree cuttings.
FIG. 4 shows the rooting rate and root number of the cuttings according to the cloning time and the cutting length conditions of the clones in the flower buds.
5 is a photograph showing the shovel receipt of the flower pot.
Fig. 6 is a photograph showing an abscissa of a flower bud rooted at a post-cedar stage.
Fig. 7 shows the growth results of the planted timber Seedlings rooted at the time of transplanting.
Fig. 8 is a photograph showing a scoop of an artificial flower implanted in porgy.

The present invention relates to a method for cultivating seedlings, which comprises the steps of (a) treating shoots collected from a flower bud mature tree with a rooting accelerator, (b) cutting the rooting accelerator-treated cuttings in a culture medium, and (c) The present invention also provides a method of growing a flowerpot using a cutting method comprising the steps of:

The method of propagating the plant using the above-described cutting method of the present invention is characterized in that the type and concentration of the rooting promoter promoting rooting is higher than that of the untreated control (untreated control) treated with the rooting agent, By establishing the cutting condition using the automatic spraying device, it is possible to provide a method of growing a flowerpot using an optimum cutting.

In the method of propagating the flower of the present invention, the plant may be 15 to 50 years old, preferably 25 to 40 years old.

In step (a) of the present invention, the step (a) may be performed by immersing the shoot collected in a flowerpot in 100 mg / L to 1000 mg / L of indolebutyric acid (IBA) as a rooting accelerator, or 1-naphthyl (1-natphtyl acetamide) powder.

In the method for growing a flower of the present invention, the shoot taken from the flower can be 1 to 2 years old and may be 10 to 15 cm or 20 to 30 cm in length.

In step (a) of the present invention, the step (a) may be carried out in an amount of 100 mg / L to 1000 mg / L of indolebutyric acid (IBA) which is a rooting accelerator at 2.5-3.5 cm, preferably 3 cm, Followed by immersion for 1 to 3 minutes, or 1-naphthylacetamide powder.

In the method of propagating the flower of the present invention, the step (b) may be performed by cutting the rooting agent treated with the rooting accelerator in the culture soil from March to June.

In the method of propagating the flower bud of the present invention, it is preferable that the cutting time in which the cutting promoter treated with the rooting accelerator is cut into the culture soil is carried out within the shortest time after the cutting is taken from the flower pot. That is, it is preferable to take the cuttings directly from the flower buds and prepare them immediately, and then treat the cuttings with rooting accelerators and immediately cut them.

In the method of propagating the flowerpot of the present invention, the culture soil may be artificial soil mixed with peatmoss: pearlite: vermiculite in a volume ratio of 1: 1.5-2.5: 1.5-2.5, preferably 1: 2: 2.

In the method for growing a flower of the present invention, the culture soil may be a scoria processed with a volcanic soil, and the clusters have good drainage characteristics.

In the method for growing a flower of the present invention, the cutting may be performed in a greenhouse without a room where an automatic mist sprayer and an automatic air circulation device are installed.

In the method for growing a flower of the present invention, the seedlings are preferably sprayed for 6 to 10 hours at a cycle of spraying for 2 minutes and spraying for 8 minutes in an unfiltered greenhouse equipped with a light-shielding film using an automatic mist sprayer And may further include an irrigation step for 8 hours.

In the method for growing a flower of the present invention, the spraying may be carried out at a speed of 0.7 l to 0.8 l / min.

In the method for growing flowerpot of the present invention, the non-pristine greenhouse can be provided with a light shielding film of 40% to 60%, preferably 50%.

In the method for growing flowerpot of the present invention, the automatic mist sprayer installed in the greenhouse can be installed for 1.5 to 2.5 minutes, preferably for 2 minutes, for 7.5 to 8.5 minutes, preferably for 8 minutes .

In addition, the present invention provides a plant grown by the above method, preferably a female plant.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, these are only for explaining the present invention in more detail, and the scope of the present invention is not limited thereto.

&Lt; Example 1 >

On March 18, 2010, six cuttings were taken from 25- to 40-year-old mature trees growing in the forest genetic resources department of the National Forestry Academy.

In the above, the shoots collected from the flower buds were prepared with 8 ~ 10cm lengths for 1-year-old branches and 13 ~ 15 cm lengths for 2-year-old leaves.

The term "two-year-olds" means a two-year-old place including a one-year-old place , and the same applies to the following examples.

Three centimeters of the base of the above-prepared cutting was immersed in a rooting accelerator mixed with 100 mg / L of indolebutyric acid (IBA) and talc powder for 2 minutes.

Immediately after the dipping, the dipped cutting was immediately cut into artificial soil mixed with peatmoss: pearlite: vermiculite in a volume ratio of 1: 2: 2.

The above-mentioned cutting was carried out in a non-pristine greenhouse equipped with a 50% light-shielding film by spraying for 2 minutes (0.72 l / min) from 9:00 am to 5:00 pm in a side watering manner using an automatic mist sprayer and an 8- So, I planted an abacus seedlings.

&Lt; Example 2 >

An explant seedling of a flowerpot was cultivated in the same manner as in Example 1 except that Scoria treated with a volcanic stone was used instead of artificial soil as a culture soil.

&Lt; Example 3 >

Six clones (Busan 3, Jeonnam 7, Jeonnam 11, Jeonnam 14, Jeonnam 20 and Chungnam 3) were planted on Jeju Island on March 15, 2011, April 30 and June 15, Respectively.

Pusan No. 3 and the like refer to the individuals that have been propagated in the selected species in each region, such as the Pusan area.

In the above, the shoots collected from the flowerpot were prepared to have a length of 10 to 15 cm for 1 year old and 20 to 30 cm for 2 years old.

An explant seedling of a flowerpot was cultivated in the same manner as in Example 3 except that Scoria treated with a volcanic stone was used instead of artificial soil as a culture soil.

&Lt; Example 5 >

An explant seedling of a flowerpot was cultivated in the same manner as in Example 3 except that the concentration of indolebutyric acid (IBA) was 1,000 mg / L.

&Lt; Example 6 >

A seedling of a flowerpot was cultivated in the same manner as in Example 5, except that a scoria treated with a volcanic stone was used instead of artificial soil as a culture soil.

&Lt; Example 7 >

The same procedure as in Example 1 was carried out except that instead of using indolebutyric acid (IBA) as a rooting accelerator, 0.4% powder of 1-natphtyl acetamide called Rooton was applied and treated So, I planted an abacus seedlings.

&Lt; Example 8 >

An explant seedling of a flowerpot was cultivated in the same manner as in Example 7 except that Scoria treated with a volcanic stone was used instead of artificial soil as a culture soil.

&Lt; Example 9 >

The same procedure as in Example 3 was conducted except that indolobutyric acid (IBA) was used as a rooting accelerator and 0.4% of 1-natphtyl acetamide powder called Rooton was used instead So, I planted an abacus seedlings.

&Lt; Example 10 >

An explant seedling of a flowerpot was cultivated in the same manner as in Example 9, except that Scoria treated with a volcanic stone was used instead of artificial soil as a culture soil.

1. Experimental Method

The rooting characteristics of the seedlings grown in Examples 1 to 10 were investigated at intervals of 5 days in order to determine when the rooting was completed.

Based on the results obtained from the previous rooting investigation, the roots were cured and the roots were fully developed and the transplantable period was selected.

The number of shoots, number of shoots, number of shoots, and number of callus were investigated by rooting of roots of 1mm or longer. Root number, root length, rooting angle, And the number of 2-sided incidence.

The results of the analysis were analyzed by the ANOVA and Duncan's multiple range test.

2. Experimental results

(One). Cutting Later period Rooting rate , Rooted Number of roots And root length changes

Root cuttings were observed from 66 days after cutting. After 101 days after cutting, both 1 - year - old and 2 - year - old rooting were completed.

Fig. 1 shows changes in rooting rate (A), number of rooted root (B) and root length (C) after cutting of a flower bud.

As shown in FIG. 1, the rooting rate of the one-year-old plant was 16.7% at the maximum and 66.7% at the maximum at the observation period. The rooting rate of at least 25.0% to 87.5% The rooting rate was higher than that of the control.

The number of roots was 1.5 ~ 12.8 in 2 - year - old plants, which was more than 1.5 ~ 3.2 in 1 - year - old. The number of roots was 2.1 on June 28 and 8.8 on 2 June.

The development of root length was better than that of 2 - year - old ground at the beginning of cutting, but the development of 2 - year - old plant was better than that of 2 - year - old at the beginning of cutting. The lobes showed 3.4 cm root length development.

At the transplanting time of the rooted cuttings, the occurrence of the secondary roots affecting the seedling growth was 81 days after cutting in the first year and 86 days after cutting in the second year.

(2). Culture soil, Shovel And Rooting agent Depending on processing conditions Cutting Root development characteristics

Table 1 below shows the roots development characteristics of the cuttings according to the treatment conditions of the cultivated soil, the shovel, and the roots treatment agent in the mature tree shoots.

In Table 1, Soil A is an artificial soil mixed with peatmoss: perlite: vermiculite in a volume ratio of 1: 2: 2, and Soil B is Scoria processed ash.

As shown in Table 1, the rooting rate of each treatment was 94.2% in scoria (Soil B) treated with artificial soil (Soil A) and volcanic stone, 95.8%, respectively.

In addition, the rooting rate of ruton treated plants was higher than that of IBA treated plants.

IBA treatments showed similar or slightly higher rooting rates than the low (100 mg / L) treatment (1,000 mg / L) treatments.

The number of roots was the highest in roots with an average of 16.2 roots, 2 year olds and roots, followed by artificial roots + 2-year roots + 15.4 roots.

In the other treatments except artificial soil + biennial treatments, more roots were developed as the IBA concentration was higher. In the artificial soil + biennial treatments, the other treatments except for the ruton showed similar root development.

Root length was the highest at 4.7cm for artificial soil + 2 year old growth + IBA (100mg / L). The average root length of artificial soil was better than that of artificial soil, but there was a clear tendency among rooting accelerators It did not appear.

Rooton> IBA (1,000 mg / L)> IBA (100 mg / L) was the most common rooting direction, similar to the development of root number. Rooton> IBA > No treatment.

The rooting rate of the double roots, which had a great effect on the development of the timber in the future, showed a large difference in each treatments. The roots of artificial root + 2 year old root roots and root roots were 91.7% , And the rooting rate was low at 5.0% and 5.8% for 1 year and 2 year old pine tree + IBA (100mg / L) treatments, respectively.

The shoot growth rate of artificially induced shoots and shoots was the highest at 80.0% and 85.8% at 2 year old + IBA (1,000 mg / L) treatments, respectively. Showed poor shoot initiation rate.

The rate of callus formation was significantly lower in Rooton treatments than in IBA treatments in all treatments.

Fig. 2 is a photograph showing an artificial soil mixed with peatmoss: pearlite: vermiculite at a volume ratio of 1: 2: 2, and a flower pot cut in a clay (volcanic stone).

(3). Cutting Time, The Length and Rooting agent Depending on processing conditions Cutting Root development characteristics

The following Tables 2, 3 and 3 show the roots development characteristics of the cuttings according to the cutting time, cutting length and rooting agent treatment conditions in the flower bud mature tree cuttings.

FIG. 4 shows the rooting rate and root number of the cuttings according to the cloning time and the cutting length conditions of the clones in the flower buds.

FIG. 5 is a photograph showing the length of the image of the flower, and FIG. 6 is a photograph showing the image of the flower of the flower bud rooted at each post-cedar period.

As shown in Tables 2 to 3 and FIG. 3 to FIG. 4, the cutting time, cutting length, and auxin treatment showed significant differences in cutting time, cutting time, auxin treatment, and clone length.

Rooting rate and number of roots were 46.8% in March, 13.7 in March, 1.5-2 times higher than in April or June, 79.5% in roux, 18.8 in poison treatment, and 67.9 in Busan 3 between clones %, And 16.4, respectively.

The cutting length was better than 15cm (45.3%, 15.0) than 20cm (41.6%, 12.3), but there was no significant difference.

Considering rooting rate and rooting characteristics, the combination of the best cutting conditions for large scale growth of the flower buds showed a cutting length of 15cm in the untouched greenhouse with mist sprayer in March.

(4). Rooted Artist Shrimp Growth at transplant time

Fig. 7 shows the results of growth of the planted shoots at the time of transplantation, and Fig. 8 is a photograph showing the seedlings of the planted flowers of the plant.

As shown in FIG. 7, the mean survival rate of the transplanted seedlings at the rooting clone was lower than that of the grafting on July 7 (69.7%, March) and on August 18 (90.2%, April) It is estimated that the rainy season continued after the transplant in July.

In July, transplantation rate was less than 15cm in 73.9% and 20cm or more in 65.4%, and in August transplantation, 95.3% was less than 15cm and 85.1% was more than 20cm.

The rate of clone startering was highest in Busan 3 in July (89.7%) and August (94.0%).

The average number of grafted tombs in July was 22.9cm, which was better than that of 17.9cm in August.

These results suggest that cutting time, shoveling date, type and concentration of rooting accelerator are influenced by root cutting, root cutting, root cutting, In the unfiltered greenhouse equipped with a 50% shading film, irrigation was carried out with an automatic spraying device to induce rooting, followed by curing, followed by growing in July, and planting in the following year. Mass proliferation is possible.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood that various modifications and changes may be made in the present invention.

By using the cutting method as a silk propagation method according to the present invention, it is possible to maintain the same qualities of genetically same in a flower color as it is, and it is possible to shorten the production period of the seedling in the spread of the good variety developed through breeding And can be mass-produced at a low cost, so that the present invention can be applied to a technical field to which the present invention belongs.

Claims

(a). Treating the shoots collected from the planted mature trees with a rooting accelerator;
(b). Cutting the cutting treated with the rooting accelerator into a culture soil; And
(c). And growing the scalloped seedlings.

The method according to claim 1, wherein the step (a) is performed by immersing the shoot collected from the flower bud in 100 mg / L to 1000 mg / L of indolebutyric acid (IBA) as a root growth promoter or 1-naphthylacetamide (1- natphtyl < / RTI > acetamide) powder.

[2] The method according to claim 1, wherein the cuttings collected from the flowerpot are 1 to 2 years old and have a length of 10 to 15 cm or 20 to 30 cm.

[3] The method according to claim 2, further comprising the step of immersing indomethacin (IBA) 100 mg / L to 1000 mg / L as rooting accelerator for 1 to 3 minutes at 2.5 to 3.5 cm of bases of the shoots collected from the flower buds, Wherein the plant is buried in a powder.

[2] The method of claim 1, wherein the step (b) is performed by cutting a rooting-promoting agent in a culture soil from March to June.

[3] The method according to claim 1, wherein the cultivated soil is an artificial soil mixed with peatmoss: pearlite: vermiculite at a volume ratio of 1: 1.5 to 2.5: 1.5 to 2.5.

[2] The method according to claim 1, wherein the cultivated soil is a scoria processed with a volcanic ash.

The method according to claim 1, wherein the cutting is carried out in a greenhouse free of charge in which an automatic mist sprayer and an automatic air circulation device are installed.

The method according to claim 1, wherein the seedlings are seedlings that have been cut in a culture soil and are sprayed in a greenhouse with a light shielding film in an automatic mist spraying system for 1.5 to 2.5 minutes and a period of 7.5 to 8.5 minutes for 6 to 10 hours Wherein the method further comprises the step of cultivating the plant.

[10] The method according to claim 9, wherein the spraying is performed at a rate of 0.7 l to 0.8 l / min.

10. A plant grown by the method of any one of claims 1 to 10.