KR20160006030A - CoA 아실화 알데히드 데히드게나제 활성이 증가된 신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법 - Google Patents

CoA 아실화 알데히드 데히드게나제 활성이 증가된 신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법 Download PDF

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KR20160006030A
KR20160006030A KR1020140085356A KR20140085356A KR20160006030A KR 20160006030 A KR20160006030 A KR 20160006030A KR 1020140085356 A KR1020140085356 A KR 1020140085356A KR 20140085356 A KR20140085356 A KR 20140085356A KR 20160006030 A KR20160006030 A KR 20160006030A
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남태욱
송여주
송승훈
윤지애
이진숙
추헌수
최인석
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Abstract

3-HPA로부터 3-HP-CoA 및 AA-CoA를 거쳐, AA를 생성하는 경로의 활성이 증가된, 아크릴산 생산능을 갖는 미생물 및 그를 이용하여 아크릴산을 생산하는 방법을 제공한다.

Description

CoA 아실화 알데히드 데히드게나제 활성이 증가된 신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법{Microorganism having novel acrylic acid synthesis pathway having enhanced activity of CoA acylating aldehyde dehydrogenase and method of producing acrylic acid using the same}
신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법에 관한 것이다.
최근 원유가격의 급등에 의한 불안정성과 탄소배출저감이 글로벌 이슈화됨에 따라 기존 석유를 원료로 하여 화학 공정을 거쳐 생산하던 연료나 화학물질들을 탄소중립적인 생물학적 공정으로 대체하여 생산하려는 노력들이 지속되고 있다.
아크릴산은 10조 원 이상의 시장을 지닌 벌크 화합물 (bulk chemical)이다. 최근에는 친환경적 생산 방법에 대한 요구로 석유계 이외의 경로를 통한 아크릴산 생산 방법의 필요성이 커지고 있다.
비석유계 아크릴산 생산 경로로는, 글리세롤 또는 포도당으로부터 3-히드록시프로피온산 (3-HP)를 생산하고, 이를 화학적으로 분리 및 정제하는 방법이 있을 수 있다. 그러나, 이 방법은 생산된 3-HP를 배양물로부터 분리하고, 정제한 후 촉매를 사용하여 화학적으로 전환하는 단계를 포함한다. 따라서, 3-HP 생산 비용에 대하여 분리, 정제 및 전환 비용이 부가되어, 석유계 화합물 유래 아크릴산 생산법에 경쟁력이 높지 않을 수 있다.
종래의 기술에 의하더라도, 아크릴산 생산능을 갖는 대안적 미생물 및 이를 이용한 아크릴산 생산방법이 요구된다.
일 양상은 유전적으로 조작되지 않은 세포에 비하여 증가된 아크릴레이트 생산능을 갖는 미생물을 제공하는 것이다.
다른 양상은 상기 미생물을 배지 중에서 배양하는 단계;를 포함하는, 아크릴레이트를 생산하는 방법을 제공한다.
본 명세서에서 사용된 용어 효소 또는 폴리펩티드 또는 단백질의 "활성 증가" 또는 "증가된 활성"은 효소 또는 폴리펩티드 또는 단백질이 활성을 나타낼 수 있도록 충분한 정도로 증가된 것일 수 있으며, 세포 또는 단리된 폴리펩티드가 비교 가능한 동일 종의 세포 또는 그의 본래 폴리펩티드에서 측정된 활성 수준과 비교하여 높은 활성 수준을 나타냄을 의미한다. 즉 해당 폴리펩티드의 활성이 본래 조작되지 않은 폴리펩티드에 의한 동일한 생화학적 활성보다 약 5% 이상, 약 10% 이상, 약 15% 이상, 약 20% 이상, 약 30% 이상, 약 50% 이상, 약 60% 이상, 약 70% 이상, 또는 약 100% 이상 증가된 것일 수 있다. 증가된 활성을 갖는 폴리펩티드는 당업계에 공지된 임의의 방법을 사용하여 확인될 수 있다.
폴리펩티드의 활성 증가는 폴리펩티드의 발현증가 또는 비활성 (specific activity)의 증가에 의하여 얻을 수 있다. 상기 발현 증가는 폴리펩티드를 코딩하는 폴리뉴클레오티드가 세포에 도입되거나 세포 내 카피 수가 증가되거나, 또는 상기 폴리뉴클레오티드의 조절 영역의 변이에 의한 것일 수 있다. 외부에서 도입되거나 또는 카피 수가 증가되는 폴리뉴클레오티드는 내인성 (endogenous) 또는 외인성 (exogenous)일 수 있다. 상기 내인성 유전자는 미생물 내부에 포함된 유전물질 상에 존재하던 유전자를 말한다. 외인성 유전자는 숙주 세포 게놈으로 도입 (integration)되는 등의 숙주 세포 내로 유전자가 도입되는 것을 의미하며, 도입되는 유전자는 도입되는 숙주세포에 대해 동종 (homologous) 또는 이종 (heterologous)일 수 있다.
용어 "카피 수 증가 (copy number increase)"는 상기 유전자의 도입 또는 증폭에 의한 것일 수 있으며, 조작되지 않은 세포에 존재하지 않는 유전자를 유전적 조작에 의해 갖게 되는 경우도 포함한다. 상기 유전자의 도입은 벡터와 같은 비히클을 매개하여 이루어질 수 있다. 상기 도입은 상기 유전자가 게놈에 통합되지 않은 임시적 (transient) 도입이거나 게놈에 삽입되는 것일 수 있다. 상기 도입은 예를 들면, 목적하는 폴리펩티드를 코딩하는 폴리뉴클레오티드가 삽입된 벡터를 상기 세포로 도입한 후, 상기 벡터가 세포 내에서 복제되거나 상기 폴리뉴클레오티드가 게놈으로 통합됨으로써 이루어질 수 있다.
용어 "유전자 (gene)"는 특정 단백질을 발현하는 핵산 단편을 의미하며, 코딩영역 또는 코딩영역 외 5'-비코딩 서열 (5'-non coding 서열)과 3'-비코딩 서열 (3'-non coding 서열) 등의 조절 (regulatory) 서열을 포함할 수 있다. 상기 조절 영역은 프로모터, 인핸서, 오퍼레이터, 리보좀 결합 부위, polyA 결합 서열, 터미네이터 영역 등을 포함할 수 있다.
"이종성 (heterologous)"은 천연 (native)이 아닌 외인성 (foreign)을 의미한다.
"분비 (secretion)"는 물질이 세포 내부에서 주변세포질공간 (periplasmic space)이나 세포 외 환경으로 이동되는 것을 의미한다.
"세포 (cell)", "균주 (strain)", 또는 "미생물 (microorganism)"은 교체 사용이 가능한 것으로서, 박테리아, 효모, 곰팡이 등을 포함한다.
"아크릴산 (acrylic acid)"은 아크릴산 또는 아크릴레이트, 또는 그 염을 포함하며 이들 서로 교환가능하게 사용될 수 있다. 아크릴산은 미생물의 발효 또는 효소 반응에 의해 생산될 수 있다.
효소 또는 폴리펩티드의 "활성의 감소" 또는 "감소된 활성"은 세포 또는 단리된 효소 또는 폴리펩티드가 비교 가능한 동일 종의 세포 또는 그의 본래 폴리펩티드에서 측정된 활성 수준과 비교하여 낮은 활성 수준을 나타내거나 활성을 나타내지 않는 것을 의미한다. 즉 해당 폴리펩티드의 활성이 본래 조작되지 않은 폴리펩티드에 의한 동일한 생화학적 활성보다 약 10%이상, 약 20%이상, 약 30%이상, 약 40%이상, 약 50% 이상, 약 55% 이상, 약 60% 이상, 약 70% 이상, 약 75% 이상, 약 80% 이상, 약 85% 이상, 약 90% 이상, 약 95% 이상, 또는 약 100% 감소된 것일 수 있다. 감소된 효소 활성은 당업계에 공지된 임의의 방법을 사용하여 확인될 수 있다. 상기 활성의 감소는 효소가 발현되더라도 효소의 활성이 없거나 감소된 경우 또는 효소를 코딩하는 유전자가 발현되지 않거나 발현되더라도 본래 조작이 되지 않은 유전자에 비하여 발현량이 감소된 경우를 포함한다.
상기 효소의 활성이 감소되는 것은 상기 효소를 코딩하는 유전자의 제거 또는 파괴에 의한 것일 수 있다. 유전자의 "제거 (deletion)" 또는 "파괴 (disruption)"는 유전자가 발현되지 않거나 발현량이 감소되거나 발현되어도 효소 활성을 나타내지 않거나 활성이 감소되도록, 유전자의 일부 또는 전부가, 또는 그 프로모터, 그 터미네이터 영역 등의 조절 인자의 일부 또는 전부가 변이, 치환, 삭제되거나 유전자에 하나 이상의 염기가 삽입되는 것을 말한다. 상기 유전자의 제거 또는 파괴는 상동 재조합과 같은 유전자 조작, 돌연변이 유발, 분자 진화를 통해 달성될 수 있다. 세포가 복수 개의 같은 유전자를 포함하거나 2개 이상의 다른 폴리펩티드 동종상동유전자 (paralog)를 포함하는 경우, 하나 또는 그 이상의 유전자가 제거 또는 파괴될 수 있다.
본 발명의 핵산 또는 폴리펩티드의 "서열 동일성 (sequence identity)"은 특정 비교 영역에서 양 서열을 최대한 일치되도록 얼라인시킨 후 서열간의 염기 또는 아미노산 잔기의 동일한 정도를 의미한다. 서열 동일성은 특정 비교 영역에서 2개의 서열을 최적으로 얼라인하여 비교함으로써 측정되는 값으로서, 비교 영역 내에서 서열의 일부는 대조 서열 (reference sequence)과 비교하여 부가 또는 삭제되어 있을 수 있다. 서열 동일성 백분율은 예를 들면, 비교 영역 전체에서 두 개의 최적으로 정렬된 서열을 비교하는 단계, 두 서열 모두에서 동일한 아미노산 또는 핵산이 나타나는 위치의 갯수를 결정하여 일치된 (matched) 위치의 갯수를 수득하는 단계, 상기 일치된 위치의 갯수를 비교 범위 내의 위치의 총 갯수 (즉, 범위 크기)로 나누는 단계, 및 상기 결과에 100을 곱하여 서열 동일성의 백분율을 수득하는 단계에 의해 계산될 수 있다. 상기 서열 동일성의 퍼센트는 공지의 서열 비교 프로그램을 사용하여 결정될 수 있으며, 상기 프로그램의 일례로 BLASTN (NCBI), CLC Main Workbench (CLC bio), MegAlignTM (DNASTAR Inc) 등을 들 수 있다.
여러 종의 동일하거나 유사한 기능이나 활성을 가지는 폴리펩티드 또는 폴리뉴클레오티드를 확인하는데 있어서 여러 수준의 서열 동일성을 사용할 수 있다. 예를 들어, 50%이상, 55%이상, 60%이상, 65%이상, 70%이상, 75%이상, 80%이상, 85%이상, 90%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상 또는 100% 등을 포함하는 서열 동일성이다.
일 양상은 유전적으로 조작되지 않은 세포에 비하여 3-히드록시프로피온알데히드 (3-HPA)를 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 CoA acylating aldehyde dehydrogenase (ALDH), 및 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 3-HP-CoA 데히드라타제의 활성이 증가되어 있는, 아크릴레이트 생산능을 갖는 미생물을 제공한다.
상기 ALDH는 EC 1.2.1.10, 또는 EC 1.2.1.87에 속하는 것일 수 있다. 상기 ALDH는 3-히드록시프로피온알데히드 (3-HPA)를 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것일 수 있다. 상기 ALDH는 서열번호 1 내지 20의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 ALDH를 코딩하는 폴리뉴클레오티드는 서열번호 1 내지 20의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다. 상기 ALDH를 코딩하는 폴리뉴클레오티드는 서열번호 21 내지 40의 뉴클레오티드 서열과 95%이상의 서열 동일성을 갖는 것일 수 있다. 상기 ALDH는 표 1 및 2에 나타낸 것 중 하나 이상인 것일 수 있다. 상기 ALDH는 그 명칭과 관계없이, 하기 반응을 촉매하는 것일 수 있다. 상기 ALDH는 CoA-acylating propionaldehyde dehydrogenase, aldehyde dehydrogenase, alcohol dehydrogenase, CoA-dependent aldehyde dehydrogenase 또는 그 조합일 수 있다. 상기 ALDH는 pduP, 예를 들면, Lactobacillus reuteri 유래의 pduP일 수 있다.
3-HPA + CoA + NAD(P)+ -> 3-HP-CoA + NAD(P)H
번호 EC 카테고리 소스균주 유전자명 구입처 서열*
1 1.2.1.10 50S ribosomal protein L29 Lactobacillus reuteri DSM 20016 Lreu_1735 KCTC 3594 1/21
2 1.2.1.10 CoA-dependent propionaldehyde dehydrogenase Lactobacillus brevis ATCC 367 LVIS_1603 ATCC 367 2/22
3 1.2.1.10 aldehyde dehydrogenase Pediococcus acidilactici HMPREF9024_01049 KCTC 1626 3/23
4 1.2.1.10 CoA-dependent propionaldehyde dehydrogenase Pediococcus claussenii ATCC BAA-344 pduP DSM 14800 4/24
5 1.2.1.10 PduP protein Lactobacillus collinoides pduP KCTC 5050 5/25
6 1.2.1.10 CoA-dependent propionaldehyde dehydrogenase Listeria welshimeri serovar 6b str. SLCC5334 NC_008555.1:1134599..1136008 ATCC 35897 6/26
7 1.2.1.10 hypothetical protein lin1129 Listeria innocua Clip11262 NC_003212.1:1144168..1145577 ATCC 33090 7/27
8 1.2.1.10 propanediol utilization Co-A dependent propionaldehyde dehydrogenase Listeria monocytogenes ATCC 19117 pduP ATCC 19117 8/28
9 1.2.1.10 ethanolamine utilization protein EutE Listeria marthii FSL S4-120 NT05LM_1376 ATCC BAA-1595 9/29
10 1.2.1.10 putative ethanolamine utilization protein EutE Listeria ivanovii subsp. ivanovii PAM 55 LIV_1097 ATCC BAA-678 10/30
11 1.2.1.10 CoA-dependent propionaldehyde dehydrogenase Listeria seeligeri serovar 1/2b str. SLCC3954 pduP ATCC 35967 11/31
12 1.2.1.10 aldehyde dehydrogenase Shewanella putrefaciens CN-32 NC_009438.1:221466..222860 ATCC BAA-453 12/32
13 1.2.1.10 aldehyde dehydrogenase family protein Kosakonia radicincitans DSM 16656 Y71_5889 DSM 16656 13/33
14 1.2.1.10 Aldehyde Dehydrogenase Tolumonas auensis DSM 9187 NC_012691.1:1861535..1862938 DSM 9187 14/34
15 1.2.1.10 hypothetical protein CKO_00785 Citrobacter koseri ATCC BAA-895 NC_009792.1:757825..759210 ATCC BAA-895 15/35
16 1.2.1.10 propanediol utilization CoA-dependent propionaldehyde dehydrogenase Yersinia enterocolitica subsp. enterocolitica 8081 NC_008800.1:2975153..2976541 ATCC 9610 16/36
17 1.2.1.10 aldehyde dehydrogenase EutE Salmonella enterica subsp. enterica serovar Mbandaka str. ATCC 51958 SEEM1958_22984 ATCC 51958 17/37
18 1.2.1.10 putative propanediol utilization protein:CoA-dependent propionaldehyde dehydrogenase Yersinia mollaretii ATCC 43969 ymoll0001_15900 ATCC 43969 18/38
19 1.2.1.10 CoA-dependent proprionaldehyde dehydrogenase pduP Escherichia fergusonii ATCC 35469 NC_011740.1:2070780..2072162 ATCC 35469 19/39
20 1.2.1.10 putative CoA-dependent proprionaldehyde dehydrogenase Salmonella enterica subsp. enterica serovar Urbana str. ATCC 9261 eutE ATCC 9261 20/40
* 서열은 아미노산 서열번호/뉴클레오티드 서열번호를 나타낸다.
상기 3-HP-CoA 데히드라타제는 EC 4.2.1.17, EC 4.2.1.55, 및 EC 4.2.1.166를 포함한, EC 4.2.1.-에 속하는 것일 수 있다. 상기 3-HP-CoA 데히드라타제는 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것일 수 있다. 상기 3-HP-CoA 데히드라타제는 서열번호 41 내지 119의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 3-HP-CoA 데히드라타제를 코딩하는 폴리뉴클레오티드는 서열번호 41 내지 119의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다. 상기 3-HP-CoA 데히드라타제를 코딩하는 폴리뉴클레오티드는 서열번호 120 내지 198의 뉴클레오티드 서열과 95%이상의 서열 동일성을 갖는 것일 수 있다. 상기 3-HP-CoA 데히드라타제는 표 3 내지 표 6에 나타낸 것 중 하나 이상인 것일 수 있다. 표 3 내지 표 6에 나타낸 효소는 E2 타입일 수 있다. 표 3 내지 6에서 "서열*"은 아미노산 서열번호/뉴클레오티드 서열번호를 나타낸다.
번호 EC 카테고리 소스 균주 유전자 구입체 서열*
1 4.2.1.- 3-hydroxybutyryl-CoA dehydratase(Crotonase) Dictyostelium discoideum (Slime mold) Q869N6 DSM947 41/120
2 4.2.1.55 3-hydroxybutyryl-CoA dehydratase(Crotonase) Clostridium acetobutylicum crt CA_C2712 KCTC1790 42/121
3 4.2.1.55 3-hydroxybutyryl-CoA dehydratase(Crotonase) Clostridium difficile crt ech KCTC5009 43/122
4 4.2.1.55 3-hydroxybutyryl-CoA dehydratase(Crotonase) Clostridium pasteurianum F502_09038 KCTC1674 44/123
5 4.2.1.55 3-hydroxybutyryl-CoA dehydratase(Crotonase) Clostridium pasteurianum F502_06297 KCTC1674 45/124
6 4.2.1.55 3-hydroxybutyryl-CoA dehydratase(Crotonase) Megasphaera elsdenii MELS_1449 KCTC5187 46/125
7 4.2.1.116 3-hydroxybutyryl-CoA dehydratase(Crotonase) Metallosphaera sedula Msed_2001 DSM5348 47/126
8 4.2.1.55 3-hydroxybutyryl-CoA dehydratase(Crotonase) Clostridicum kluyvery crt1 DSM555 48/127
9 4.2.1.- 4-hydroxybutyryl-CoA dehydratase Sulfolobus tokodaii STK_16590 DSM16993 49/128
10 4.2.1.- 4-hydroxybutyryl-CoA dehydratase Geobacter metallireducens Gmet_2215 DSM7210 50/129
11 4.2.1.- 4-hydroxybutyryl-CoA dehydratase Sulfolobus solfataricus abfD-1 DSM1617 51/130
12 4.2.1.- 4-hydroxybutyryl-CoA dehydratase Syntrophobacter fumaroxidans Sfum_3141 DSM10017 52/131
13 4.2.1.- 4-hydroxybutyryl-CoA dehydratase Porphyromonas gingivalis PGN_0727 DSM20709 53/132
14 4.2.1.- 4-hydroxybutyryl-CoA dehydratase Polynucleobacter necessarius subsp. Asymbioticus Pnuc_0370 DSM18221 54/133
15 4.2.1.116 3-hydroxypropionyl-CoA dehydratase Sulfolobus tokodaii STK_15160 DSM16993 55/134
16 4.2.1.- 3-hydroxypropionyl-CoA dehydratase Gordonia terrae C-6 GTC6_11571 KCTC9807 56/135
17 4.2.1.- 3-hydroxypropionyl-CoA dehydratase Halalkalicoccus jeotgali HacjB3_17558 C497_07209 DSM18796 57/136
18 4.2.1.- 3-hydroxypropionyl-CoA dehydratase Carboxydothermus hydrogenoformans CHY_1739 DSM6008 58/137
19 4.2.1.55 3-hydroxypropionyl-CoA dehydratase Thermomicrobium roseum trd_0041 DSM5159 59/138
20 4.2.1.17 3-hydroxypropionyl-CoA dehydratase Methylobacterium extorquens croA METDI5699 DSM1337 60/139
번호 EC 카테고리 소스 균주 유전자 구입처 서열*
21 4.2.1.- R-phenyllactate dehydratase Clostridium sporogenes


fldB KCTC5654 61/140
22 4.2.1.- R-phenyllactate dehydratase fldC KCTC5654 62/141
23 4.2.1.- R-phenyllactate dehydratase fldI KCTC5654 63/142
24 4.2.1.- R-phenyllactate dehydratase fldA KCTC5654 64/143
25 4.2.1.- R-phenyllactate dehydratase Lachnoanaerobaculum saburreum

fldC HMPREF0381_2734 DSM3986 65/144
26 4.2.1.- R-phenyllactate dehydratase fldB HMPREF0381_2735 DSM3986 66/145
27 4.2.1.- R-phenyllactate dehydratase fldI2 HMPREF0381_2736 DSM3986 67/146
28 4.2.1.- R-phenyllactate dehydratase Peptostreptococcus stomatis

fldI HMPREF0634_1391 DSM17678 68/147
29 4.2.1.- R-phenyllactate dehydratase HMPREF0634_1028 DSM17678 69/148
30 4.2.1.- R-phenyllactate dehydratase fldB HMPREF0634_1029 DSM17678 70/149
31 4.2.1.- 2-hydroxyisocaproyl-CoA dehydratase Clostridium difficile


hadB KCTC5009 71/150
32 4.2.1.- 2-hydroxyisocaproyl-CoA dehydratase hadC KCTC5009 72/151
33 4.2.1.- 2-hydroxyisocaproyl-CoA dehydratase hadI KCTC5009 73/152
34 4.2.1.- 2-hydroxyisocaproyl-CoA dehydratase hadA KCTC5009 74/153
35 4.2.1.17 Enoyl-CoA hydratase Escherichia coli (strain K12) paaF 보유 75/154
36 4.2.1.17 Enoyl-CoA hydratase Rhodobacter capsulatus fadB1 KCTC2583 76/155
37 4.2.1.- Enoyl-CoA hydratase Pseudomonas stutzeri PSTAA_0117 DSM4166 77/156
38 4.2.1.- Enoyl-CoA hydratase Haliangium ochraceum Hoch_4602 DSM14365 78/157
39 4.2.1.- Enoyl-CoA hydratase Anoxybacillus flavithermus Aflv_0566 DSM21510 79/158
40 4.2.1.- Enoyl-CoA hydratase Streptomyces avermitilis echA3 SAV_717 DSM46492 80/159
41 4.2.1.- Enoyl-CoA hydratase Advenella kashmirensis TKWG_10020 DSM17095 81/160
번호 EC 카테고리 소스 균주 유전자 구입처 서열*
42 4.2.1.- Enoyl-CoA hydratase Oligotropha carboxidovorans OCA5_c12950 OCAR_6780 DSM1227 82/161
43 4.2.1.- Enoyl-CoA hydratase Riemerella anatipestifer Riean_1526 RA0C_1812 DSM15868 83/162
44 4.2.1.- Enoyl-CoA hydratase Fusobacterium necrophorum subsp. funduliforme Fnf 1007

HMPREF1127_1435 DSM19678 84/163
45 4.2.1.- Enoyl-CoA hydratase HMPREF1127_1434 DSM19678 85/164
46 4.2.1.- Enoyl-CoA hydratase HMPREF1127_1436 DSM19678 86/165
47 4.2.1.- Enoyl-CoA hydratase Desulfosporosinus youngiae DSM 17734

DesyoDRAFT_3696 DSM17734 87/166
48 4.2.1.- Enoyl-CoA hydratase DesyoDRAFT_3695 DSM17734 88/167
49 4.2.1.- Enoyl-CoA hydratase DesyoDRAFT_3697 DSM17734 89/168
50 4.2.1.- Enoyl-CoA hydratase Peptoniphilus indolicus ATCC 29427

fldB HMPREF9129_0353 KCTC15023 90/169
51 4.2.1.- Enoyl-CoA hydratase HMPREF9129_0354 KCTC15023 91/170
52 4.2.1.- Enoyl-CoA hydratase HMPREF9129_0352 KCTC15023 92/171
53 4.2.1.- Enoyl-CoA hydratase Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10)

Desmer_1800 DSM13257 93/172
54 4.2.1.- Enoyl-CoA hydratase Desmer_1801 DSM13257 94/173
55 4.2.1.- Enoyl-CoA hydratase Desmer_1799 DSM13257 95/174
56 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Acidaminococcus fermentans

hgdA Acfer_1815 DSM20731 96/175
57 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase hgdB Acfer_1815 DSM20731 97/176
58 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase hgdC Acfer_1815 DSM20731 98/177
59 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Carboxydothermus hydrogenoformans

hgdB CHY_0846 DSM6008 99/178
60 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase hgdA CHY_0847 DSM6008 100/179
61 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase hgdC CHY_0848 DSM6008 101/180
62 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Oscillibacter valericigenes

hgdC OBV_10870 DSM18026 102/181
63 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase hgdA OBV_10880 DSM18026 103/182
64 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase hgdB OBV_10890 DSM18026 104/183
번호 EC 카테고리 소스 균주 유전자 구입처 서열*
65 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) (Desulfotomaculum orientis)

Desor_3092 DSM765 105/184
66 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Desor_3093 DSM765 106/185
67 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Desor_3091 DSM765 107/186
68 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Peptostreptococcus anaerobius CAG:621

BN738_00824 KCTC5182 108/187
69 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase BN738_00823 KCTC5182 109/188
70 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase BN738_00825 KCTC5182 110/189
71 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Chloroflexus aggregans (strain MD-66 / DSM 9485) Cagg_1174 DSM9485 111/190
72 4.2.1.17 2-hydroxyglutaryl-CoA dehydratase Marivirga tractuosa (strain ATCC 23168 / DSM 4126 / NBRC 15989 / NCIMB 1408 / VKM B-1430 / H-43) (Microscilla tractuosa) (Flexibacter tractuosus) Ftrac_3721 KCTC2958 112/191
73 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) Marky_1278 DSM14884 113/192
74 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034) Cpin_6304 KCTC3412 114/193
75 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Megasphaera elsdenii DSM 20460 MELS_0744 KCTC5187 115/194
76 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Megasphaera elsdenii DSM 20460 MELS_0745 KCTC5187 116/195
77 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Megasphaera elsdenii DSM 20460 MELS_0746 KCTC5187 117/196
78 4.2.1.- 2-hydroxyglutaryl-CoA dehydratase Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) Chy400_0108 DSM635 118/197
79 4.2.1.- enoyl-CoA hydrastase Ruegeria pomeroyi DSS-3 SP00147 DSM15171 119/198
상기 미생물은 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소의 활성이 더 증가되어 있는 것일 수 있다.
상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 EC 3.1.2.4를 포함한, EC 3.1.2-에 속하는 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 3-HP-CoA hydrolase, 또는 3-hydroxyisobutyryl-CoA hydrolase인 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 활성이 그 역반응을 촉매하는 것보다 높은 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 서열번호 199 내지 204의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소를 코딩하는 폴리뉴클레오티드는 서열번호 199 내지 204의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소를 코딩하는 폴리뉴클레오티드는 서열번호 205 내지 210의 뉴클레오티드 서열과 95%이상의 서열 동일성을 갖는 것일 수 있다. 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 표 7에 나타낸 것 중 하나 이상인 것일 수 있다. 표 7에 나타낸 효소는 E3 타입일 수 있다. 표 7에서, "서열*"은 아미노산 서열번호/뉴클레오티드 서열번호를 나타낸다.
번호 EC 카테고리 소스 균주 유전자명 구입 서열*
1 3.1.2.- Acyl - CoA thioester hydrolase E. coli yciA 보유 199/205
2 3.1.2.- Acyl-CoA thioester hydrolase Klebsiella oxytoca 10-5245 HMPREF9689_01673 KCTC1686 200/206
3 3.1.2.- Acyl-CoA thioester hydrolase Cronobacter turicensis yciA 보유 201/207
4 3.1.2.- Acyl-CoA thioester hydrolase Citrobacter freundii D186_20262 보유 202/208
5 3.1.2.- Acyl-CoA thioester hydrolase Salmonella enterica SeI_A1458 DSM5569 203/209
6 3.1.2.- Acyl-CoA thioester hydrolase Shigella flexneri 1235-66 SF123566_2028 보유 204/210
상기 미생물은 유전적으로 조작되지 않은 세포에 비하여 상기 2개 효소 또는 3개 효소의 유전자의 발현이 증가되도록 유전적으로 조작된 미생물일 수 있다. 상기 3개 효소의 활성이 모(parent) 세포에 이미 존재하는 경우에는 유전적 조작을 통해 상기 2개 효소 또는 3개 효소의의 발현을 더욱 증가시킬 수 있거나, 야생형 미생물에 존재하지 않는 경우에는 유전적 조작의 방법으로 상기 2개 효소 또는 3개 효소를 코딩하는 유전자를 모세포에 도입하여 발현 또는 과발현되도록 할 수 있다. 상기 유전적으로 조작되지 않은 세포는 야생형 또는 상기 미생물이 유래된 모세포를 의미한다.
상기 2개 또는 3개 효소 유전자의 발현 또는 과발현은 당업자에게 알려진 여러 방법에 의해 달성될 수 있다. 일례로 유전자 카피 수를 증가시키거나, 유도제 (inducer) 또는 억제제 (repressor)와 같은 조절 물질을 사용하여 발현을 증가시킬 수 있다. 상기 카피 수 증가는 상기 유전자의 도입 또는 증폭에 의한 것일 수 있다. 즉, 작동조절 가능하도록 연결된 조절 인자 및 상기 2개 또는 3개 효소 유전자를 포함하는 벡터, 발현 카세트 등을 숙주세포에 도입함으로써 달성될 수 있다.
또는 2개 또는 3개 효소의 활성의 증가는 상기 유전자의 발현 조절 서열의 변형에 의한 것일 수 있다. 상기 조절 서열은 상기 유전자 발현을 위한 프로모터 서열 또는 전사 종결자 서열일 수 있다. 또한, 상기 조절 서열은 유전자 발현에 영향을 줄 수 있는 모티프를 코딩하는 서열일 수 있다. 상기 모티프는 예를 들면, 이차 구조-안정화 모티프, RNA 불안정화 모티프, 스플라이스-활성화 모티프, 폴리아데닐화 모티프, 아데닌-풍부 서열 (adenine-rich sequence), 또는 엔도뉴클레아제 인식 부위일 수 있다.
상기 미생물은 박테리아, 효모, 및 곰팡이로 이루어진 군으로부터 선택될 수 있다. 예를 들면, 에세리키아 (Escherichia), 루멘박테리아, 코리네박테리움 (Corynebacterium) 속 및 브레비박테리움 (Brevibacterium) 속으로 구성된 군으로부터 선택되는 것일 수 있다. 상기 세포는 코리네박테리움 속일 수 있다. 상기 미생물은 대장균 (E.coli), 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum), 코리네박테리움 써모아미노게네스 (Corynebacterium thermoaminogenes), 브리비박테리움 플라붐 (Brevibacterium flavum) 및 브리비박테리움 락토페르멘툼 (Brevibacterium lactofermentum)으로 구성된 군으로부터 선택되는 미생물일 수 있다.
상기 미생물은 아크릴산을 천연으로 생산하거나 또는 재조합 방법으로 아크릴산을 생산하도록 유전적으로 조작된 것일 수 있다. 또한, 상기 미생물은 3-HPA 생산능을 갖는 것일 수 있다. 상기 미생물이 천연적으로 3-HPA를 생산하지 하지 않는 경우, 3-HPA를 생산하도록 유전적으로 조작된 것일 수 있다. 상기 미생물은 글리세롤을 3-프로피온산 알데히드 (3-propionic aldehyde: 3-HPA)로 전환하는 반응을 촉매하는 효소를 코딩하는 유전자가 도입되어 있어, 3-HPA 생산능을 갖는 것일 수 있다. 상기 미생물은 예를 들면, 대장균을 포함한 에세리키아 속 미생물일 수 있다. 상기 글리세롤을 3-HPA로 전환하는 반응을 촉매하는 효소는 글리세롤 데히드라타제 (glycerol dehydratase: GDH)일 수 있다.
상기 GDH는 글리세롤을 3-HPA로의 전환을 촉매하는 것이면 어느 것이 포함될 수 있다. 상기 GDH는 EC 4.2.1.30 또는 디올 데히드라타제 (EC 4.2.1.28)에 속하는 것일 수 있다. 상기 GDH 및 그를 코딩하는 뉴클레오티드는 Ilyobacter polytropus, Klebsiella pneumoniae, Citrobacter freundii, Clostritidium pasteurianum, Salmonella typhimurium, 또는 Klebsiella oxytoca로부터 유래한 것일 수 있다. 각 경우에 있어서, 상기 GDH는 세 개 서브유닛으로 구성될 수 있다: 큰 (large) 또는 "α" 서브유닛, 중간 (medium) 또는 "β" 서브유닛, 및 작은 (small) 또는 "γ" 서브유닛. GDH의 큰 (large) 또는 "α" 서브유닛을 코딩하는 유전자는 dhaB1, gldA 및 dhaB를 포함할 수 있다. 중간 (medium) 또는 "β" 서브유닛를 코딩하는 유전자는 dhaB2, gldB 및 dhaC를 포함할 수 있다. 작은 (small) 또는 "γ" 서브유닛을 코딩하는 유전자는 dhaB3, gldC 및 dhaE를 포함할 수 있다. 디올 데히드라타제의 큰 (large) 또는 "α" 서브유닛을 코딩하는 유전자는 pduC 및 pddA를 포함할 수 있다. 디올 데히드라타제의 중간 (medium) 또는 "β" 서브유닛를 코딩하는 유전자는 pduD 및 pddB를 포함할 수 있다. 디올 데히드라타제의 작은 (small) 또는 "γ" 서브유닛을 코딩하는 유전자는 pduE 및 pddC를 포함할 수 있다. 표 8 및 표 9는 GDH 및 GDH 연결된 기능에 대한 유전자 명칭 및 GenBank 참조를 비교한 것이다. 상기 GDH는 Ilyobacter polytropus 유래의 dhaB1, dhaB2, 및 dhaB3를 포함하는 것일 수 있다. Ilyobacter polytropus 유래의 DhaB1, DhaB2, 및 DhaB3는 각각 서열번호 211,212,및 213의 아미노산 서열을 갖는 것일 수 있다. dhaB1 유전자, dhaB2 유전자, 및 dhaB3 유전자는 각각 서열번호 211,212,및 213의 아미노산 서열을 코딩하는 것일 수 있다. Ilyobacter polytropus 유래의 dhaB1 유전자, dhaB2 유전자, 및 dhaB3 유전자는 각각 서열번호 214, 215, 및 216을 갖는 것일 수 있다.
개체
(GenBank참조번호)

유전자 기능
조절
미지
재활성화
(reactivation)
미지
유전자 염기쌍 유전자 염기쌍 유전자 염기쌍 유전자 염기쌍
K.pneumoniae(U30903) orf2c 7116-7646 orf2b 6762-7115 orf2a 5125-5556
K.pneumoniae(U60992) GdrB
C.freundii(U09771) dhaR 3746-5671 orfW 5649-6179 orfX 6180-6533 orfY 7736-8164
C.pasteurianum(AF051373)
C.pasteurianum(AF026270) orfW 210-731 orfX 1-196 orfY 746-1177
S.typhimurium(AF026270) pduH 8274-8645
K.oxytoca(AF017781) DdrB 2063-2440
K.oxytoca(AF051373)
개체
(GenBank참조번호)

유전자 기능
데히드라타제,α 데히드라타제,α
데히드라타제,α 재활성화
(reactivation)
유전자 염기쌍 유전자 염기쌍 유전자 염기쌍 유전자 염기쌍
K.pneumoniae(U30903) dhaB1 3047-4714 dhaB2 2450-2890 dhaB3 2022-2447 orf2a 186-2009
K.pneumoniae(U60992) gldA 121-1788 gldB 1801-2382 gldB 2388-2813 gdrA
C.freundii(U09771) dhaB 8556-10223 dhaC 10235-10819 dhaC 10822-11250 orfY 11261-13072
C.pasteurianum(AF051373) dhaB 84-1748 dhaC 1779-2318 dhaC 2333-2773 2790-4598
C.pasteurianum(AF026270) orfY
S.typhimurium(AF026270) pduC 3557-5221 pduD 5232-5906 pduD 5921-6442 6452-8284
K.oxytoca(AF017781) 241-2073
K.oxytoca(AF051373) pddA 121-1785 pddB 1796-2470 pddB 2485-3006
상기 GDH는 Ilyobacter polytropus 유래의 dhaB1, dhaB2, 및 dhaB3의 각 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다.
상기 미생물은 글리세롤 데하이드라타제 재활성화효소 (glycerol dehydratase reactivase: GDR)를 코딩하는 폴리뉴클레오티드를 더 포함하는 것일 수 있다. 글리세롤 및 디올 데히드라타제는 글리세롤 및 다른 일부 기질에 의한 기작-근거한 자살 불활성화의 대상이 된다 (Daniel et al., FEMS Microbiol. Rev. 22, 553(1999)). 용어 "글리세롤 데하이드라타제 재활성화효소 (glycerol dehydratase reactivase: GDR)"는 상기 데히드라타제 활성을 재활성화하는 단백질을 나타낸다. 용어 "데히드라타제 재활성화 활성 (dehydratase reactivating activity)"은 기질을 촉매할 수 없는 데히드라타제를 기질을 촉매할 수 있는 것으로 전환하는 현상 또는 데히드라타제의 저해를 억제하는 현상 또는 인 비보에서 데히드라타제 효소의 유용한 반감기 (half-life)를 연장시키는 현상을 나타낸다. 상기 GDR은 dhaB, gdrA, pduG 및 ddrA 중 하나 이상일 수 있다. 또한, GDR은 orfX, orf2b, gdrB, pduH 및 ddrB 중 하나 이상일 수 있다.
상기 GDR은 K.pneumoniae (U60992) 유래의 gdrA 및 gdrB로서 각각 서열번호 217 및 218의 아미노산 서열을 갖는 것일 수 있다. 또는, 상기 GDR은 Ilyobacter polytropus 유래의 gdrA 및 gdrB로서 각각 서열번호 219 및 220의 아미노산 서열을 갖는 것일 수 있다. 상기 GDR은 서열번호 217 내지 220의 아미노산 서열과 각각 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. GdrA 및 GdrB을 코딩하는 유전자는 각각 서열번호 217 내지 220의 아미노산 서열을 코딩하는 서열을 갖는 것, 예를 들면, 서열번호 221 내지 224의 각 뉴클레오티드 서열을 갖는 것일 수 있다.
상기 미생물에 있어서, GDH를 코딩하는 폴리뉴클레오티드, 및 GDR을 코딩하는 폴리뉴클레오티드 중 하나 이상은 유전적으로 조작되지 않은 미생물에 비하여 더 높은 수준으로 발현되는 것일 수 있다. 상기 발현 수준은 mRNA 또는 단백질 수준의 발현일 수 있다. 상기 단백질 수준의 발현은 발현된 단백질의 양 또는 활성에 근거한 것일 수 있다. 상기 발현 수준은 약 5% 이상, 약 10% 이상, 약 15% 이상, 약 20% 이상, 약 30% 이상, 약 50% 이상, 약 60% 이상, 약 70% 이상, 약 100% 이상, 200% 이상, 또는 300% 이상 증가된 것일 수 있다.
상기 미생물은 3-HPA 생산능을 갖는 것일 수 있다. 상기 미생물에 있어서, 상기 발현 증가는 유전적으로 조작되지 않은 미생물에 비하여 더 높은 수준의 3-HPA를 생산하도록 하는 것일 수 있다. 상기 3-HPA의 생산은 세포 내에서 생산되는 것, 세포 내에서 생산되어 세포 외부로 분비되는 것, 또는 그 조합을 포함한다. 세포 내에서 생산된 3-HPA은 아크릴산과 같은 다른 대사 산물로부터 전환될 수 있다. 상기 3-HPA 생산은 약 5% 이상, 약 10% 이상, 약 15% 이상, 약 20% 이상, 약 30% 이상, 약 50% 이상, 약 60% 이상, 약 70% 이상, 약 100% 이상, 200% 이상, 또는 300% 이상 증가된 것일 수 있다.
상기 발현 증가는 폴리펩티드를 코딩하는 폴리뉴클레오티드가 세포에 도입되거나 세포 내 카피 수가 증가되거나, 또는 상기 폴리뉴클레오티드의 조절 영역의 변이에 의한 것일 수 있다. 외부에서 도입되거나 또는 카피 수가 증가되는 폴리뉴클레오티드는 내인성 (endogenous) 또는 외인성 (exogenous)일 수 있다. 상기 내인성 유전자는 미생물 내부에 포함된 유전물질 상에 존재하던 유전자를 말한다. 외인성 유전자는 숙주 세포 게놈으로 도입 (integration)되는 등의 숙주 세포 내로 유전자가 도입되는 것을 의미하며, 도입되는 유전자는 도입되는 숙주세포에 대해 동종 (homologous) 또는 이종 (heterologous)일 수 있다.
상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소의 활성이 감소된 것일 수 있다. 상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소를 코딩하는 유전자가 제거 또는 파괴되어 있는 것일 수 있다.
또한, 상기 미생물은 아크릴레이트를 다른 산물로 전환하는 경로를 더 포함하는 것일 수 있다. 상기 미생물에 있어서, 아크릴산의 생산은 세포내, 또는 세포 내에서 생성되어 분비되는 것일 수 있다. 따라서, 상기 미생물은 아크릴산을 세포 내에 생성하고, 이를 다른 산물로 전환하는데 관여하는 경로, 예를 들면, 효소 유전자 및 그 발현물을 더 포함할 수 있다. 상기 다른 산물은 아크릴레이트 에스테르일 수 있다.
상기 미생물은 피루베이트로부터 락테이트를 합성하는 경로가 불활성화 또는 감쇄된 것일 수 있다. 상기 미생물은 락테이트 데히드로게나아제(lactate dehydrogenase, LDH)의 활성이 제거되거나 감소된 것일 수 있다. 상기 LDH는 피루베이트를 락테이트로 전환하는 반응을 촉매하는 활성을 가질 수 있다. 상기 LDH는 EC.1.1.1.27로 분류되는 효소일 수 있다. 일례로 상기 LDH는 서열번호 225의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 포함할 수 있다. 상기 미생물은 락테이트 데히드로게나아제를 코딩하는 유전자가 파괴 또는 제거된 것일 수 있다. 상기 LDH 유전자는 서열번호 225의 아미노산 서열과 65%이상, 예를 들어, 70%이상, 80%이상, 85%이상, 90%이상, 91%이상, 92%이상, 93%이상, 94%이상, 95%이상, 96%이상, 97%이상, 98%이상, 99%이상, 또는 100%의 서열 동일성을 가지는 아미노산 서열을 코딩하는 것일 수 있다.
다른 양상은 상기한 바와 같은 미생물을 배지 중에서 배양하는 단계;를 포함하는, 아크릴레이트를 생산하는 방법을 제공한다.
상기 배양은 당업계에 알려진 적당한 배지와 배양조건에 따라 이루어질 수 있다. 통상의 기술자라면 선택되는 미생물에 따라 배지 및 배양조건을 용이하게 조정하여 사용할 수 있다. 배양 방법은 회분식, 연속식, 유가식, 또는 이들의 조합 배양을 포함할 수 있다. 상기 미생물은 아크릴레이트를 세포 외부로 분비하는 것일 수 있다.
상기 배지는 다양한 탄소원, 질소원 및 미량원소 성분을 포함할 수 있다.
상기 탄소원은, 예를 들면, 포도당, 자당, 유당, 과당, 말토오스, 전분, 셀룰로오스와 같은 탄수화물, 대두유, 해바라기유, 피마자유, 코코넛유와 같은 지방, 팔미트산, 스테아린산, 리놀레산과 같은 지방산, 글리세롤 및 에탄올과 같은 알코올, 아세트산과 같은 유기산, 또는 이들의 조합을 포함할 수 있다. 상기 배양은 글루코스를 탄소원으로 하여 수행될 수 있다. 상기 질소원은, 펩톤, 효모 추출물, 육즙, 맥아 추출물, 옥수수 침지액 (CSL), 및 대두밀과 같은 유기 질소원 및 요소, 황산암모늄, 염화암모늄, 인산암모늄, 탄산암모늄 및 질산암모늄과 같은 무기 질소원, 또는 이들의 조합을 포함할 수 있다. 상기 배지는 인의 공급원으로서, 예를 들면, 인산이수소칼륨, 인산수소이칼륨 및 상응하는 소듐-함유 염, 황산마그네슘 또는 황산철과 같은 금속염을 포함할 수 있다. 또한, 아미노산, 비타민, 및 적절한 전구체 등이 배지에 포함될 수 있다. 상기 배지 또는 개별 성분은 배양액에 회분식, 유가식 또는 연속식으로 첨가될 수 있다.
또한, 배양 중에 달리 pH를 조절하지 않거나, 수산화암모늄, 수산화칼륨, 암모니아, 인산 및 황산과 같은 화합물을 미생물 배양액에 적절한 방식으로 첨가하여 배양액의 pH를 조정할 수 있다. 또한, 배양 중에 지방산 폴리글리콜 에스테르와 같은 소포제를 사용하여 기포 생성을 억제할 수 있다.
상기 배양은 미호기성 조건에서 배양하는 것일 수 있다. 용어 "미호기성 조건 (microaerobic condition)"은 정상 대기상태보다 산소의 함량이 적은 양의 산소를 가진 공기가 배양액과 접촉하는 상태에서 배양액에 공급되는 산소의 양을 의미한다. 미호기성 조건은 예를 들면, 대기중 공기에 이산화탄소 또는 질소를 약 0.1 내지 0.4 vvm, 약 0.2 내지 0.3 vvm, 또는 약 0.25 vvm의 유속으로 공급하여 조성될 수 있다. 또한, 미호기성 조건은 통기 속도가 약 0 내지 0.4 vvm, 약 0.1 내지 0.3 vvm, 또는 약 0.15 내지 0.25 vvm인 것일 수 있다. 상기 배양은 글리세롤 예를 들면, 1-20중량%, 1-10중량% 또는 2-10중량%를 포함하는 배지 중에서 배양하는 것일 수 있다.
상기 방법은 배양물로부터 아크릴레이트를 회수하는 단계를 더 포함할 수 있다. 상기 회수는 세포 또는 세포를 제외한 배양액, 또는 둘 모두에서 분리될 수 있다. 배양물로부터 아크릴산의 분리는 당업계에 알려진 분리 및 정제방법을 사용하여 수행될 수 있다. 상기 회수는 원심분리, 크로마토그래피, 추출, 여과, 침전, 또는 이들의 조합에 의하여 이루어질 수 있다.
상기 방법에 있어서, 상기 미생물은 아크릴레이트를 다른 산물로 전환하는 경로를 더 포함하는 것이고, 생산된 아크릴레이트를 다른 산물로 전환하는 단계를 더 포함하는 것일 수 있다. 다른 산물은 폴리아크릴레이트를 포함한 아크릴레이트 에스테르인 것일 수 있다.
일 양상에 따른 미생물에 의하면, 3-아크릴산의 증가된 생산능을 갖는다.
다른 양상에 따른 아크릴산을 생산하는 방법에 의하면, 아크릴산을 효율적으로 생산할 수 있다.
도 1은 pET-iBAB_PduP 벡터의 개열 지도를 나타낸다.
도 2는 글리세롤 함유 배지에서 대장균 SH3, 및 ALDH 유전자와 3-HP-CoA dehydratase 유전자가 도입된 두 재조합 대장균 균주를 48시간 배양한 경우, 배양물 중의 아크릴레이트를 질량 분석기에 의하여 분석한 결과를 나타낸다.
도 3은 48 시간 경과시에 도 1에서 측정된 아크릴레이트의 양을 나타낸다.
도 4는 대장균 SH3와 SH3/pET-iBAB-eP(PduP)/pACYC-E2(#12) 균주를 발효기 (fermentor)에서 48 시간 동안 배양한 후 배양액 중의 아크릴레이트를 측정한 결과를 나타낸다.
도 5는 대장균 SH3와 SH3/pET-iBAB-eP(PduP)/pACYC-E2(#12) 균주를 발효기 (fermentor)에서 48 시간 동안 배양한 후 배양액 중의 3-HP를 측정한 결과를 나타낸다.
도 6은 일 실시예의 대장균에서 포도당 또는 글리세롤로부터 아크릴산의 생산의 예측 경로를 나타낸 도면이다.
이하 본 발명을 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
재료 및 방법
달리 언급이 없으면, 이하 실시예에서는 다음의 재료 및 방법이 사용되었다.
(1) 3- HPA 생산능을 갖는 대장균 세포의 제조
3-HPA 생산능을 갖는 대장균 균주 즉, E.coli K12 (DE3) (ㅿyqhD ㅿack-pta/pET-iBAB)를 다음과 같은 과정에 의하여 제조하였다. ackA-pta 및 yqhD 유전자가 결실된 균주는 Red recombinase 효소 발현에 의한 방법으로 다음과 같은 과정으로 제작되었다. 우선, ackA-pta를 결실시키기 위해 pKD4 벡터 (서열번호 226)를 주형으로 하고 ackAKF 프라이머 (서열번호 227) 및 ackAKR 프라이머 (서열번호 228)의 프라이머 세트를 사용한 PCR 증폭에 의해 45 bp의 ackA-pta 유전자 양 말단과 상동성을 가지는 증폭 산물을 얻었다. 이 DNA를 Red recombinase를 발현하는 pKD46 벡터 (서열번호 229)를 가지는 대장균 K12 (DE3) 균주에 전기천공 (electroporation)에 의하여 도입하여 카나마이신 항생제에 저항성 (KmR)을 가지는 균주를 선별한 후 해당 균주의 게놈의 ackA-pta 유전자 부위가 카나마이신 항생제에 대한 저항성을 부여하는 유전자로 치환되었음을 확인하였다.
여기서 얻어진 균주에 고온에서 발현되는 Flp recombinase의 유전자를 가지는 pCP20 벡터 (서열번호 230)를 도입하여 Flp recombinase를 발현시켜 게놈 내부 KmR 유전자를 제거한 후, PCR을 통해 ackA-pta 유전자가 결실되고 KmR 유전자를 가지지 않은 균주를 얻었음을 확인하였다. 동일한 실험과정을 통해, pKD4 벡터를 주형으로 하고 yqhDKF 프라이머(서열번호 231) 및 yqhDKR 프라이머(서열번호 232)의 프라이머 세트를 통한 PCR 증폭 산물을 상기 ackA-pta 유전자가 결실되고 KmR 유전자를 가지지 않은 균주에 도입한 후 KmR 유전자를 제거하여 최종적으로 ackA-pta 및 yqhD 유전자가 결실된 SH3 균주를 획득하였다.
pET-iBAB 벡터는 다음의 과정으로 제작하였다.
Ilyobacter polytropus의 게놈 DNA로부터 글리세롤 데하이드라타제 (glycerol dehydratase: GDH)를 코딩하는 유전자 (dhaB1, dhaB2, 및 dhaB3) (서열번호 214, 215,및 216) 및 글리세롤 데히드라타제 재활성화효소 인자 (glycerol dehydratase reactivase: GDR)를 코딩하는 유전자 (gdrA, 및 gdrB)(서열번호 223 및 224)를 확보하였다. 상기 dhaB1, dhaB2, 및 dhaB3 유전자는 Ilyobacter polytropus의 게놈 DNA를 주형으로 하고 dhaB123_F (서열번호 233) 및 dhaB123_R(서열번호 234)의 프라이머 세트를 사용한 PCR 증폭에 의하여 dhaB123를 한 증폭 산물로 얻었다. 상기 gdrA 및 gdrB 유전자는 Ilyobacter polytropus의 게놈 DNA를 주형으로 하고 gdrAB_F(서열번호 235) 및 gdrAB_R(서열번호 236)의 프라이머 세트를 사용한 PCR 증폭에 의하여 gdrAB를 한 증폭 산물로 얻었다. 얻어진 PCR 산물을 BamHI 및 SacI 제한효소로 처리한 후, pETDuetTM-1 벡터 (Novagen, Cat. No. 71146-3)에 클로닝하여 pET-iBAB 벡터를 얻었다.
(2) 3- HPA 생산능을 갖는 대장균 세포에 ALDH 및 3- HP - CoA 데히드라타제 유전자가 도입된 균주의 제조
글리세롤로부터 3-HPA를 거쳐 3-HP-CoA를 생산하기 위한 벡터 (pET-iBAB-PduP)는 다음의 과정을 거쳐 제작되었다. 상기 pET-iBAB 벡터를 주형으로 하여 iBAB_Up 및 iBAB_Dn 프라이머 세트(서열번호 237 및 238)를 사용한 PCR 증폭에 의해 dhaB123 및 gdrAB를 포함하는 선형의 벡터를 얻었다. PCR은 Primestar Max (Takara Inc., R045A)를 사용하여 95℃에서 15초, 50℃에서 15초, 및 72℃에서 2분을 30회 (cycle) 수행하였다. 또한, Lactobacillus reuteri DSM 20016의 게놈 DNA로부터 CoA acylating aldehyde dehydrogenase (ALDH)를 코딩하는 유전자 (PduP)를 pduP_F 및 pduP_R의 프라이머 세트(서열번호 239 및 240)를 사용한 PCR 증폭에 의하여 얻었다. 얻어진 PCR 산물을 In-FusionTM HD Cloning Kit (Clontech Laboratories, Inc.)를 사용하여 상기의 선형 벡터에 클로닝하였다. 그 결과, pET-iBAB_PduP (pETDuet-1/dhaB_gdrAB_pduP) 벡터를 얻었다.
도 1은 pET-iBAB_PduP 벡터의 개열 지도를 나타낸다.
E.coli K12 (DE3) (ㅿyqhD ㅿack-pta/pET-iBAB-PduP)에 3-HP-CoA 데히드라타제 유전자로서 MELS_1449 유전자를 도입하였다.
구체적으로, Megasphaera elsdenii 균주의 게놈을 주형으로 하고 HindIII 및 BamHI 자리를 가진 각 프라이머 세트 (서열번호 241 및 242)를 사용하여 PCR을 통하여 증폭하였으며, PCR은 Primestar Max (Takara Inc., R045A)를 사용하여 95℃에서 15초, 50℃에서 15초, 및 72℃에서 2분을 30회 (cycle) 수행하였다. 얻어진 각 증폭 산물을 HindIII 및 BamHI으로 소화시키고, 이를 pACYCDuetTM-1 벡터 (Novagen, cat. no. 71147-3)의 HindIII 및 BamHI 자리에 연결시켜 pACYC-MDH 벡터를 제조하였다.
pET-iBAB-PduP 벡터 및 pACYC-MDH 벡터를 대장균 SH3 균주에 전기천공에 의하여 도입하였다. 구체적으로, 전기 천공용으로 제작된 0.05mL의 SH3 세포액에 두 벡터를 200 내지 300ng이 되도록 넣어준 후, electroporation cuvette (Bio-rad Inc., cat. No. 165-2802)에 넣고 Gene Pulser XcellTM Total System (Bio-rad Inc., cat. No. 165-2660)을 사용하여 2.5 kV의 pulse를 인가하여 형질도입하였다. 이렇게 형질 도입된 세포에서 카나마이신 항생제 및 클로람페니콜 항생제에 동시에 내성을 가지는 균주를 선별하여, 최종적으로 SH3/pET-iBAB-PduP/pACYC-MDH 균주를 제조하였다.
(3) 3- HPA 생산능을 갖는 대장균 세포에 ALDH 및 3- HP - CoA 데히드라타제 및 아크릴릴- CoA 아크릴레이트로 전환하는 것을 촉매하는 효소 유전자가 도입된 균주의 제조
E.coli K12 (DE3) (ㅿyqhD ㅿack-pta/pET-iBAB-PduP)에 3-HP-CoA 데히드라타제 및 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소 유전자로서 M.elsdenii 유래의 3-HP-CoA dehydratase를 코딩하는 MELS_1449 유전자, 및 대장균 유래의 CoA hydrolase yciA 유전자를 도입하였다.
구체적으로, 대장균 유래의 CoA hydrolase yciA 유전자를 대장균 (K12 MG1655)의 게놈을 주형으로 하고, yciA_F 및 yciA_R 프라이머 세트 (서열번호 243 및 244)를 사용하여 PCR로 증폭하여 얻고, 이를 BglII 및 XhoI 제한 효소를 사용하여 각각 소화시킨 후, 동일한 효소로 소화된 pACYC-MDH 벡터에 도입하여, 2개 유전자 발현용 벡터 (pACYC-MDH-yciA)를 제조하였다.
다음으로, (2)에 기재된 pET-iBAB-PduP 벡터 및 pACYC-MDH-YciA 벡터를 대장균 SH3/pET-iBAB-PduP/pACYC-MDH 균주를 제작할 때와 동일한 방법으로 electroporation 기법으로 형질도입하였으며, 카나마이신 항생제 및 클로람페니콜 항생제에 동시에 내성을 가지는 균주를 선별하여, 대장균 SH3/pET-iBAB-PduP/pACYC-MDH-YciA 균주를 제조하였다.
실시예 1: ALDH , 및 3- HP - CoA 아크릴릴 - CoA ( acrylyl - CoA )로 전환하는 것을 촉매하는 3- HP - CoA 데히드라타제 유전자가 도입된 미생물의 아크릴레트 생산성의 확인
재료 및 방법에 제조된 대장균 SH3, SH3/pET-iBAB-PduP/pACYC-MDH 균주 및 SH3/pET-iBAB-PduP/pACYC-MDH-YciA 균주를 각각 250mL 플라스크에서 RM 최소 배지 (MgSO4 ㆍ7H2O 1.4 g/L, K2HPO4 17.4 g/L, KH2PO4 3g/L, (NH4)2HPO4 4 g/L, 시트르산 1.7 g/L, ZnCl2 0.014 g/L, FeCl2ㆍ4H2O 0.041 g/L, MnCl2 0.015 g/L, CuCl2 0.0015 g/L, H3BO3 0.003 g/L, Na2MoO4 0.0025 g/L, 비타민 B12 10 uM, 포도당 1.0 g/L, 및 글리세롤 30 g/L) 20mL에서 OD600=0.25가 되게 접종하고 30℃에서 OD600=0.6이 될 때까지 배양한 후 0.03mM IPTG를 첨가하고 33℃에서 48 시간 동안 배양하였다. 배양은 220mL 플라스크에서 진탕하면서 48 시간 동안 이루어졌다.
다음으로, 배양물 중의 아크릴산 및 기타 유기산의 농도는 HPLC를 사용하여 측정하였다. 구체적으로, 배양이 종료된 후, 배양액 중 일부를 채취하여 흡광도를 측정하고, 세포가 제외된 배양액에 대하여 굴절율 검출기(refractive index detector) 및 포토다이오드 어레이 검출기 (photodiode array detector)가 부착된 HPLC (Waters)에 Aminex HPX-87H 컬럼을 장착하여 5 mM H2SO4 수용액을 사용, 0.1 ml/min의 flow rate로 흘려주어 아크릴레이트(AA)가 생산되는지를 확인하였다. 생성된 AA의 정량은 포토다이오드 검출기의 210 nm 파장에서 정제된 AA 시료 (Sigma Aldrich)와의 정량 비교를 통해 이루어졌다. HPLC 분석 결과, ALDH 유전자와 3-HP-CoA dehydratase 유전자가 도입된 두 재조합 대장균 균주, 즉 SH3/pET-iBAB-PduP/pACYC-MDH 균주 및 SH3/pET-iBAB-PduP/pACYC-MDH-YciA 균주는 48 시간 동안 배양한 경우, 약 6 mg/L의 아크릴산을 생산하였다 (도 2 참조).
도 2는 글리세롤 함유 배지에서 ALDH 유전자와 3-HP-CoA dehydratase 유전자가 도입된 두 재조합 대장균 균주를 48시간 배양한 경우, 배양물 중의 아크릴레이트를 HPLC에 의하여 분석한 결과를 나타낸다. 도 2에서, A는 대장균 SH3/pET-iBAB-PduP/pACYC-MDH 균주를 나타내고, B는 SH3/pET-iBAB-PduP/pACYC-MDH-YciA 균주, 및 C는 2.8mg/L 아크릴레이트 표준물을 각각 나타낸다. 도 2에서 가로축은 HPLC에 연결된 Aminex HPX-87H 컬럼에 배약액을 주입 후 0.1 ml/min의 속도로 5 mM H2SO4 수용액을 흘려 주었을 때 포토다이오드 어레이 검출기에 도달한 시간 (min)을 나타내고, 세로축은 포토다이오드 어레이 검출기에서 210 nm 파장 범위에서 측정된 전압 (uV)을 나타낸다. 아크릴레이트 농도는 아크릴레이트 표준물 기준으로 두 시료 모두 약 6 mg/L로 측정되었다.
도 3은 대장균 SH3/pET-iBAB-PduP/pACYC-MDH 균주를 발효기 (fermentor)에서 48 시간 동안 배양한 후 배양액 중의 아크릴레이트를 측정한 결과를 나타낸다. 도 3에서, 배양은 1.5 L 배양기 (Biotron)에 상기의 RM 최소 배지 600 mL에서 상기 균주를 OD600이 0.1이 되게 접종하고, 33 ℃에서 600 rpm으로 교반하면서 48 시간 동안 배양한 것이다. 도 3에 나타낸 바와 같이, SH3/pET-iBAB-PduP/pACYC-MDH 균주는 아크릴레이트를 현저하게 증가된 양으로 생산하였다. 최대 생산은 40 시간에서 44 mg/L 아크릴레이트를 생산하였다.
도 4는 본 실시예의 대장균에서 포도당 또는 글리세롤로부터 아크릴산의 생산의 예측 경로를 나타낸 도면이다. 본 실시예에서 아크릴산은 도 4에 나타낸 경로에 따라 생산될 것으로 여겨지지만, 청구된 발명이 특별한 기작에 한정되는 것은 아니다. 도 4에서, 포도당 또는 글리세롤로부터 전환된 3-HPA는 PduP에 의하여 3-HP-CoA로 전환되는 것이 촉매되며, 3-HP-CoA는 MELS_1449에 의하여 AA-CoA로 전환되는 것이 촉매된다. AA-CoA로부터 AA로의 전환은 세포에 내재적으로 존재하는 효소, 예를 들면, YciA에 의하여 촉매되거나, 외부에서 도입된 효소 유전자, 예를 들면, YciA 유전자의 발현 산물에 의하여 전환이 촉매될 수 있다. 대장균의 경우 YciA 유전자는 내재적으로 존재할 수 있으며, 그에 따라 외부에서 도입하지 않아도 AA-CoA로부터 AA로 전환될 수 있다. 탄소원, 예를 들면, 포도당 또는 글리세롤로부터 3-HPA로 전환하는 경로를 갖는 균주 즉, 3-HPA 생산능을 갖는 균주는, 본 실시예에 기재된 SH3/pET-iBAB-PduP/pACYC-MDH-YciA 균주, 및 SH3/pET-iBAB-PduP/pACYC-MDH 균주뿐만 아니라, 당업계에 알려진 균주가 사용될 수 있다.
<110> Samsung Electronics Co., Ltd. <120> Microorganism having novel acrylic acid synthesis pathway having enhanced activity of CoA acylating aldehyde dehydrogenase and method of producing acrylic acid using the same <130> PN105322KR <160> 244 <170> KopatentIn 2.0 <210> 1 <211> 477 <212> PRT <213> Lactobacillus reuteri DSM 20016 <400> 1 Met Gln Ile Asn Asp Ile Glu Ser Ala Val Arg Lys Ile Leu Ala Glu 1 5 10 15 Glu Leu Asp Asn Ala Ser Ser Ser Ser Ala Asn Val Ala Ala Thr Thr 20 25 30 Asp Asn Gly His Arg Gly Ile Phe Thr Asn Val Asn Asp Ala Ile Ala 35 40 45 Ala Ala Lys Ala Ala Gln Glu Ile Tyr Arg Asp Lys Pro Ile Ala Val 50 55 60 Arg Gln Gln Val Ile Asp Ala Ile Lys Glu Gly Phe Arg Pro Tyr Ile 65 70 75 80 Glu Lys Met Ala Lys Asp Ile Lys Glu Glu Thr Gly Met Gly Thr Val 85 90 95 Glu Ala Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly 100 105 110 Pro Glu Ile Leu Glu Pro Val Val Glu Asn Gly Asp Gly Gly Met Val 115 120 125 Met Tyr Glu Arg Leu Pro Tyr Gly Val Ile Gly Ala Val Gly Pro Ser 130 135 140 Thr Asn Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala 145 150 155 160 Gly Gly Asn Thr Leu Tyr Phe Gly Ala His Pro Gly Ala Lys Asn Val 165 170 175 Thr Arg Trp Thr Ile Glu Lys Met Asn Asp Phe Ile Ala Asp Ala Thr 180 185 190 Gly Leu His Asn Leu Val Val Ser Ile Glu Thr Pro Thr Ile Glu Ser 195 200 205 Val Gln Gln Met Met Lys His Pro Asp Ile Ala Met Leu Ala Val Thr 210 215 220 Gly Gly Pro Ala Val Val His Gln Ala Met Thr Ser Gly Lys Lys Ala 225 230 235 240 Val Gly Ala Gly Pro Gly Asn Pro Pro Ala Met Val Asp Ala Thr Ala 245 250 255 Asp Ile Asp Leu Ala Ala His Asn Ile Ile Thr Ser Ala Ser Phe Asp 260 265 270 Asn Asp Ile Leu Cys Thr Ala Glu Lys Glu Val Val Ala Glu Ser Ser 275 280 285 Ile Lys Asp Glu Leu Ile Arg Lys Met Gln Asp Glu Gly Ala Phe Val 290 295 300 Val Asn Arg Glu Gln Ala Asp Lys Leu Ala Asp Met Cys Ile Gln Glu 305 310 315 320 Asn Gly Ala Pro Asp Arg Lys Phe Val Gly Lys Asp Ala Thr Tyr Ile 325 330 335 Leu Asp Gln Ala Asn Ile Pro Tyr Thr Gly His Pro Val Glu Ile Ile 340 345 350 Cys Glu Leu Pro Lys Glu His Pro Leu Val Met Thr Glu Met Leu Met 355 360 365 Pro Ile Leu Pro Val Val Ser Cys Pro Thr Phe Asp Asp Val Leu Lys 370 375 380 Thr Ala Val Glu Val Glu Lys Gly Asn His His Thr Ala Thr Ile His 385 390 395 400 Ser Asn Asn Leu Lys His Ile Asn Asn Ala Ala His Arg Met Gln Cys 405 410 415 Ser Ile Phe Val Val Asn Gly Pro Ser Tyr Val Gly Thr Gly Val Ala 420 425 430 Asp Asn Gly Ala His Ser Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro 435 440 445 Thr Gly Glu Gly Thr Cys Thr Ala Arg Thr Phe Thr Arg Arg Val Arg 450 455 460 Leu Asn Ser Pro Gln Gly Phe Ser Val Arg Asn Trp Tyr 465 470 475 <210> 2 <211> 477 <212> PRT <213> Lactobacillus brevis ATCC 367 <400> 2 Met Asn Thr Glu Asn Ile Glu Gln Ala Ile Arg Lys Ile Leu Ser Glu 1 5 10 15 Glu Leu Ser Asn Pro Gln Ser Ser Thr Ala Thr Asn Thr Thr Val Pro 20 25 30 Gly Lys Asn Gly Ile Phe Lys Thr Val Asn Glu Ala Ile Ala Ala Thr 35 40 45 Lys Ala Ala Gln Glu Asn Tyr Ala Asp Gln Pro Ile Ser Val Arg Asn 50 55 60 Lys Val Ile Asp Ala Ile Arg Glu Gly Phe Arg Pro Tyr Ile Glu Asp 65 70 75 80 Met Ala Lys Arg Ile His Asp Glu Thr Gly Met Gly Thr Val Ser Ala 85 90 95 Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly Pro Glu 100 105 110 Ile Leu Gln Pro Glu Ala Glu Thr Gly Asp Gly Gly Leu Val Met Tyr 115 120 125 Glu Tyr Ala Pro Phe Gly Val Ile Gly Ala Val Gly Pro Ser Thr Asn 130 135 140 Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala Gly Gly 145 150 155 160 Asn Thr Leu Phe Phe Gly Ala His Pro Gly Ala Lys Asn Ile Thr Arg 165 170 175 Trp Thr Ile Glu Lys Leu Asn Glu Leu Val Ala Asp Ala Thr Gly Leu 180 185 190 His Asn Leu Val Val Ser Leu Glu Thr Pro Ser Ile Glu Ser Val Gln 195 200 205 Glu Val Met Gln His Pro Asp Val Ala Met Leu Ser Ile Thr Gly Gly 210 215 220 Pro Ala Val Val His Gln Ala Leu Ile Ser Gly Lys Lys Ala Val Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ala Met Val Asp Ala Thr Ala Asn Ile 245 250 255 Ala Leu Ala Ala His Asn Ile Val Asp Ser Ala Ala Phe Asp Asn Asn 260 265 270 Ile Leu Cys Thr Ala Glu Lys Glu Val Val Val Glu Ala Ala Val Lys 275 280 285 Asp Glu Leu Ile Met Arg Met Gln Gln Glu Gly Ala Phe Leu Val Thr 290 295 300 Asp Ser Ala Asp Ile Glu Lys Leu Ala Gln Met Thr Ile Gly Pro Lys 305 310 315 320 Gly Ala Pro Asp Arg Lys Phe Val Gly Lys Asp Ala Thr Tyr Ile Leu 325 330 335 Asp Gln Ala Gly Ile Ser Tyr Thr Gly Thr Pro Thr Leu Ile Ile Leu 340 345 350 Glu Ala Ala Lys Asp His Pro Leu Val Thr Thr Glu Met Leu Met Pro 355 360 365 Ile Leu Pro Val Val Cys Cys Pro Asp Phe Asp Ser Val Leu Ala Thr 370 375 380 Ala Thr Glu Val Glu Gly Gly Leu His His Thr Ala Ser Ile His Ser 385 390 395 400 Glu Asn Leu Pro His Ile Asn Lys Ala Ala His Arg Leu Asn Thr Ser 405 410 415 Ile Phe Val Val Asn Gly Pro Thr Tyr Cys Gly Thr Gly Val Ala Thr 420 425 430 Asn Gly Ala His Ser Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro Thr 435 440 445 Gly Glu Gly Thr Ala Thr Ser Lys Thr Tyr Thr Arg Arg Arg Arg Leu 450 455 460 Asn Ser Pro Glu Gly Phe Ser Leu Arg Thr Trp Glu Ala 465 470 475 <210> 3 <211> 477 <212> PRT <213> Pediococcus acidilactici <400> 3 Met Glu Ile Gln Asn Leu Glu Glu Asp Ile Arg Arg Ile Leu Ser Glu 1 5 10 15 Glu Leu Lys Lys Ser Gly Thr Ser Gln Thr Ala Ser Thr Ser Asp Ala 20 25 30 Gly Gln Asn Gly Ile Phe Lys Thr Val Asp Glu Ala Ile Ala Ala Ala 35 40 45 Lys Ala Ala Glu Asp Val Tyr Ile Asp Lys Pro Leu Ala Phe Arg Glu 50 55 60 Lys Val Leu Thr Ala Ile Arg Glu Gly Phe Arg Pro Tyr Ile Glu Lys 65 70 75 80 Met Ala Lys Asp Ile Lys Asp Glu Thr Gly Met Gly Thr Val Glu Ala 85 90 95 Lys Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly Thr Glu 100 105 110 Ile Leu Gln Pro Glu Ala Glu Thr Gly Asp Gly Gly Leu Val Met Tyr 115 120 125 Glu Tyr Ala Pro Phe Gly Val Ile Gly Ala Val Gly Pro Ser Thr Asn 130 135 140 Pro Ser Glu Thr Val Ile Ala Asn Ala Ile Met Met Leu Ala Gly Gly 145 150 155 160 Asn Thr Leu Tyr Phe Gly Ala His Pro Gly Ala Lys Lys Ile Thr Arg 165 170 175 Trp Thr Ile Glu Lys Leu Asn Lys Leu Val Tyr Glu Ala Thr Gly Met 180 185 190 Lys Asn Leu Val Val Ser Ile Glu Glu Pro Ser Ile Glu Ser Val Gln 195 200 205 Glu Met Met Gln His Pro Asp Ile Ala Met Leu Ser Ile Thr Gly Gly 210 215 220 Pro Ala Val Val His Gln Ala Leu Val Ser Gly Lys Lys Ala Val Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ala Ile Val Asp Ala Thr Ala Asn Val 245 250 255 Ala Leu Ala Ala His Asn Ile Val Asp Ser Ala Ser Phe Asp Asn Asn 260 265 270 Ile Leu Cys Thr Ala Glu Lys Glu Val Val Val Glu Ser Ser Val Lys 275 280 285 Asp Glu Leu Ile Lys Lys Met Gln Glu Glu Gly Ala Phe Leu Val Thr 290 295 300 Asn Ala Ser Asp Ile Asp Lys Leu Ala Glu Met Thr Ile Gly Lys Asn 305 310 315 320 Gly Ala Pro Asp Arg Gln Phe Val Gly Lys Asp Ala Thr Tyr Ile Leu 325 330 335 Asp Lys Ala Gly Ile Ala Tyr Thr Gly Thr Pro Lys Leu Ile Ile Met 340 345 350 Glu Ala Gln Lys Asp His Pro Leu Val Thr Thr Glu Met Leu Met Pro 355 360 365 Ile Val Pro Val Val Ser Cys Pro Thr Phe Asp Gln Val Leu Ala Thr 370 375 380 Ala Val Glu Val Glu Gln Gly Leu His His Thr Ala Ser Ile His Ser 385 390 395 400 Glu Asn Leu Pro Asn Ile Asn Arg Ala Ala His Arg Met Asn Thr Ser 405 410 415 Ile Phe Val Val Asn Gly Ala Thr Tyr Val Gly Thr Gly Val Gly Ala 420 425 430 Asn Gly Ala His Ala Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro Thr 435 440 445 Gly Glu Gly Thr Ala Thr Ala Lys Thr Phe Thr Arg Arg Arg Arg Leu 450 455 460 Asn Ser Pro Glu Ala Phe Ser Leu Arg Ser Trp Glu Ala 465 470 475 <210> 4 <211> 476 <212> PRT <213> Pediococcus claussenii ATCC BAA-344 <400> 4 Met Glu Met Asp Lys Leu Glu Gln Asp Ile Arg Arg Ile Leu Ser Glu 1 5 10 15 Glu Leu Gln Asp Ser Asp Asn Ser Val Ser Ala Ser Ser Asp Asn Gly 20 25 30 Thr Asn Gly Ile Phe Lys Thr Val Asp Glu Ala Ile Ala Ala Ala Lys 35 40 45 Ala Ala Gln Glu Ile Tyr Val Asp Lys Ser Leu Ala Phe Arg Asn Gln 50 55 60 Val Leu Asp Ala Ile Lys Glu Gly Phe Arg Pro Tyr Ile Glu Gln Met 65 70 75 80 Ala Lys Asp Ile Lys Glu Glu Thr Gly Met Gly Thr Val Glu Ala Lys 85 90 95 Ile Ala Lys Leu Asn Asn Ala Leu Tyr Asn Thr Pro Gly Thr Glu Ile 100 105 110 Leu Glu Pro Glu Ala Glu Thr Gly Asp Gly Gly Leu Val Leu Tyr Glu 115 120 125 Tyr Ala Pro Phe Gly Val Ile Gly Ala Val Gly Pro Ser Thr Asn Pro 130 135 140 Ser Glu Thr Val Ile Ala Asn Ala Leu Met Met Leu Ala Gly Gly Asn 145 150 155 160 Thr Val Tyr Phe Gly Ala His Pro Gly Ala Lys Lys Ile Thr Arg Trp 165 170 175 Thr Ile Glu Lys Leu Asn Glu Phe Val Phe Lys Ala Thr Gly Met Arg 180 185 190 Asn Met Val Val Ser Ile Glu Glu Pro Ser Ile Glu Ser Val Gln Gln 195 200 205 Met Met Gln His Pro Asp Ile Ala Met Leu Ser Ile Thr Gly Gly Pro 210 215 220 Gly Val Val His Gln Ala Met Ile Ser Gly Lys Lys Ala Val Gly Ala 225 230 235 240 Gly Ala Gly Asn Pro Pro Ala Ile Val Asp Ala Thr Ala Asn Ile Asp 245 250 255 Leu Ala Ala His Asn Ile Val Asp Ser Ser Ser Phe Asp Asn Asn Ile 260 265 270 Leu Cys Thr Ala Glu Lys Glu Val Val Val Glu Glu Ser Val Lys Asp 275 280 285 Glu Leu Ile Ser Lys Met Gln Asn Glu Gly Ala Phe Leu Val Thr Ser 290 295 300 Ala His Asp Ile Glu Lys Ile Val Gln Ile Thr Ile Gly Lys Asn Gly 305 310 315 320 Ala Pro Asp Arg Lys Phe Val Gly Lys Asp Ala Thr Phe Ile Leu Asp 325 330 335 Ser Ala Gly Ile Asn Tyr Thr Gly Thr Pro Lys Leu Ile Ile Leu Glu 340 345 350 Ala His Lys Asn His Pro Leu Val Thr Thr Glu Met Leu Met Pro Ile 355 360 365 Leu Pro Val Val Ser Cys Pro Thr Phe Asp Arg Ala Leu Ala Thr Ala 370 375 380 Val Glu Val Glu Gln Gly Leu His His Thr Ala Ser Ile His Ser Glu 385 390 395 400 Asn Leu Pro His Ile Asn Gln Ala Ala His Arg Met Asn Thr Ser Ile 405 410 415 Phe Val Val Asn Gly Ala Thr Tyr Val Gly Thr Gly Val Gly Ala Asn 420 425 430 Gly Ala His Ala Gly Ala Ser Ala Leu Thr Ile Ala Thr Pro Thr Gly 435 440 445 Glu Gly Thr Ala Thr Ala Lys Thr Phe Thr Arg Arg Arg Arg Leu Asn 450 455 460 Ser Pro Glu Ala Phe Ser Leu Arg Ser Trp Glu Ala 465 470 475 <210> 5 <211> 481 <212> PRT <213> Lactobacillus collinoides <400> 5 Met Ala Asp Gln Asn Ile Glu Ala Glu Ile Arg Arg Ile Leu Gln Glu 1 5 10 15 Glu Leu Ser Gly Asn Ala Ser Ser Ser Ala Ala Gly Thr Thr Thr Ser 20 25 30 Gln Pro Asp Gly Leu Gly Asn Arg Ile Phe Thr Asn Val Asn Asp Ala 35 40 45 Ile Ala Ala Ala Lys Gln Ala Gln Ala Ile Tyr Gln Asp Lys Pro Leu 50 55 60 Ala Phe Arg Lys Lys Val Val Gln Ala Ile Lys Asp Gly Phe Gly Pro 65 70 75 80 Tyr Ile Glu Tyr Met Ala Lys Gln Thr Arg Glu Glu Thr Gly Met Gly 85 90 95 Thr Ala Glu Ala Lys Ile Ala Lys Leu Lys Asn Ala Leu Tyr Asn Thr 100 105 110 Pro Gly Val Glu Leu Leu Asp Pro Glu Val Glu Thr Gly Asp Gly Gly 115 120 125 Met Val Met Tyr Glu Tyr Thr Pro Phe Gly Val Ile Gly Ala Val Gly 130 135 140 Pro Ser Thr Asn Pro Cys Glu Thr Val Leu Asn Asn Ser Ile Met Met 145 150 155 160 Met Ser Ala Gly Asn Ala Leu Phe Phe Gly Ala His Pro Gly Ala Lys 165 170 175 Asn Ile Thr Arg Trp Ala Val Glu Lys Leu Asn Glu Phe Val Tyr Lys 180 185 190 Ala Thr Gly Leu Lys Asn Leu Leu Val Ser Leu Asp Thr Pro Ser Ile 195 200 205 Glu Ser Val Gln Glu Met Met Gln His Pro Asp Val Ala Met Leu Ala 210 215 220 Val Thr Gly Gly Pro Ala Val Val His Gln Ala Leu Thr Ser Gly Lys 225 230 235 240 Lys Ala Val Gly Ala Gly Ala Gly Asn Pro Pro Ala Met Val Asp Ala 245 250 255 Thr Ala Asp Ile Asp Leu Ala Ala His Asn Leu Phe Thr Ser Ala Lys 260 265 270 Phe Asp Asn Glu Ile Leu Cys Thr Ser Glu Lys Glu Ile Ile Ala Glu 275 280 285 Asp Ser Ile Lys Asp Glu Leu Leu Gln Lys Ile Val Ala Lys Gly Ala 290 295 300 Cys Leu Val Thr Asp Pro Lys Asp Ile Lys His Leu Ala Asp Met Thr 305 310 315 320 Ile Gly Asp Asn Gly Ala Pro Asp Arg Lys Tyr Val Gly Lys Asp Ala 325 330 335 Thr Val Ile Leu Asp Ala Ala Gly Ile Ser Tyr Thr Gly Asp Pro Lys 340 345 350 Leu Ile Met Met Asp Val Asp Lys Asp Asn Pro Leu Val Lys Thr Glu 355 360 365 Met Leu Met Pro Ile Leu Pro Ile Val Gly Cys Pro Asp Phe Asp Ala 370 375 380 Val Leu Ala Thr Ala Ile Glu Val Glu Gly Gly Asn His His Thr Ala 385 390 395 400 Ser Ile His Ser Asn Asn Ile Leu His Ile Asn Lys Ala Ala His Arg 405 410 415 Met Asn Thr Ser Ile Phe Val Ala Asn Gly Pro Thr Phe Ala Ala Thr 420 425 430 Gly Val Gly Asp Asn Gly Tyr Tyr Ser Gly Ala Ala Ala Leu Thr Ile 435 440 445 Ala Thr Pro Thr Gly Glu Gly Thr Thr Thr Thr Lys Thr Phe Thr Arg 450 455 460 Arg Arg Arg Phe Asn Cys Pro Gln Gly Phe Ser Leu Arg Ser Trp Glu 465 470 475 480 Val <210> 6 <211> 469 <212> PRT <213> Listeria welshimeri serovar 6b str. SLCC5334 <400> 6 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Glu Gln Lys Asp Val Pro Val Lys Thr Thr Thr Gln Gly 20 25 30 Ala Lys Ser Gly Ile Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Gln Ala Gln Asn Ser Tyr Lys Glu Lys Ser Leu Glu Glu Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Glu Ser 65 70 75 80 Ile Ala Thr Arg Ala Val Ala Glu Thr Gly Met Gly Asn Val Thr Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Thr Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Glu Ser Cys Gly Ile 180 185 190 Asp Asn Leu Val Val Thr Val Glu Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Val Val Ala Val Asp Ser Ile Thr 275 280 285 Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr 290 295 300 Asn Pro Ser Asp Ile Lys Lys Leu Glu Lys Val Ala Val Thr Asp Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Ser Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Thr Cys Asp Phe Thr Pro Arg Leu Ile Ile Val 340 345 350 Glu Thr Asp Lys Ser His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Val Val Arg Val Pro Asp Phe Asp Glu Ala Leu Lys Val 370 375 380 Ala Ile Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Arg Gly 420 425 430 Glu Gly Ser Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 7 <211> 469 <212> PRT <213> Listeria innocua Clip11262 <400> 7 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Glu Gln Lys Glu Val Pro Thr Lys Thr Thr Thr Gln Gly 20 25 30 Ala Lys Ser Gly Val Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Ile Ala Gln Asn Cys Tyr Lys Glu Lys Ser Leu Glu Glu Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Glu Thr 65 70 75 80 Ile Ala Thr Arg Ala Val Ala Glu Thr Gly Met Gly Asn Val Thr Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Ser Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Asp Ser Cys Gly Ile 180 185 190 Asp Asn Leu Ile Val Thr Val Ala Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Val Val Ala Val Asp Ser Ile Ala 275 280 285 Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr 290 295 300 Asn Pro Ser Asp Ile Gln Lys Leu Glu Lys Val Ala Val Thr Asp Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Thr Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Ala Cys Asp Phe Thr Pro Arg Leu Ile Ile Val 340 345 350 Glu Thr Glu Lys Ser His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Val Val Arg Val Pro Asp Phe Asp Glu Ala Leu Glu Val 370 375 380 Ala Ile Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Arg Gly 420 425 430 Glu Gly Ser Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 8 <211> 469 <212> PRT <213> Listeria monocytogenes ATCC 19117 <400> 8 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Glu Gln Lys Asp Ala Pro Val Lys Thr Thr Val Lys Gly 20 25 30 Ala Lys Ser Gly Val Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Ile Ala Gln Asn Asn Tyr Lys Glu Lys Ser Leu Glu Glu Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Glu Ser 65 70 75 80 Ile Ala Ala Arg Ala Val Ala Glu Thr Gly Met Gly Asn Val Ala Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Thr Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Glu Ser Cys Gly Ile 180 185 190 Asp Asn Leu Val Val Thr Val Glu Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Ile Val Ala Val Asp Ser Ile Ala 275 280 285 Asp Phe Leu Met Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr 290 295 300 Asn Pro Ser Asp Ile Gln Lys Leu Glu Lys Val Ala Val Thr Asp Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Ser Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Val Cys Asp Phe Ser Pro Arg Leu Ile Ile Val 340 345 350 Glu Thr Glu Lys Thr His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Val Val Arg Val Pro Asn Phe Asp Glu Ala Leu Asp Val 370 375 380 Ala Ile Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Arg Gly 420 425 430 Glu Gly Ser Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 9 <211> 469 <212> PRT <213> Listeria marthii FSL S4-120 <400> 9 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Glu Gln Lys Glu Ala Pro Ala Lys Pro Ile Thr Gln Gly 20 25 30 Ala Lys Ser Gly Ile Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Ile Ala Gln Asn Cys Tyr Lys Glu Lys Ser Leu Glu Glu Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Thr Leu Tyr Pro Glu Ile Glu Thr 65 70 75 80 Ile Ala Thr Lys Ala Val Ala Glu Thr Gly Met Gly Asn Val Ala Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Thr Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Glu Ser Cys Gly Ile 180 185 190 Asp Asn Leu Val Val Thr Val Glu Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Ile Val Ala Val Glu Ser Ile Ala 275 280 285 Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr 290 295 300 Asn Pro Ser Asp Ile Gln Lys Leu Glu Lys Val Ala Val Thr Asp Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Ala Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Thr Cys Asp Phe Thr Pro Arg Leu Ile Ile Val 340 345 350 Glu Thr Thr Lys Thr His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Leu Val Arg Val Pro Asp Phe Asp Glu Ala Leu Glu Val 370 375 380 Ala Ile Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Arg Gly 420 425 430 Glu Gly Ser Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 10 <211> 469 <212> PRT <213> Listeria ivanovii subsp. ivanovii PAM 55 <400> 10 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Gly Gln Asn Glu Glu Thr Pro Lys Lys Pro Ser Gln Gly 20 25 30 Ala Lys Ser Gly Ile Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Ile Ala Gln Asn Cys Tyr Lys Glu Lys Ser Leu Glu Asp Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Glu Asn 65 70 75 80 Ile Ala Thr Arg Ala Ala Ala Glu Thr Gly Met Gly Asn Val Ala Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Thr Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Glu Ser Cys Gly Ile 180 185 190 Asp Asn Leu Val Val Thr Val Glu Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Ala Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Val Val Ala Val Asp Ser Ile Thr 275 280 285 Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Phe His Val Thr 290 295 300 Asn Pro Ser Asp Ile Arg Lys Leu Glu Lys Val Ala Val Thr Glu Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Ser Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Ala Cys Asp Phe Thr Pro Arg Leu Ile Ile Ala 340 345 350 Glu Thr Asp Arg Ser His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Val Val Arg Val Ala Asp Phe Asp Gln Ala Leu Glu Val 370 375 380 Ala Leu Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Gly Gly 420 425 430 Glu Gly Ser Ala Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 11 <211> 469 <212> PRT <213> Listeria seeligeri serovar 1/2b str. SLCC3954 <400> 11 Met Glu Ser Leu Glu Leu Glu Gln Leu Val Lys Lys Val Leu Leu Glu 1 5 10 15 Lys Leu Ala Gly Gln Asn Glu Glu Thr Pro Lys Lys Pro Ser Gln Gly 20 25 30 Ala Lys Ser Gly Ile Phe Asp Thr Val Asp Glu Ala Val Gln Ala Ala 35 40 45 Val Ile Ala Gln Asn Cys Tyr Lys Glu Lys Ser Leu Glu Asp Arg Arg 50 55 60 Asn Val Val Lys Ala Ile Arg Glu Ala Leu Tyr Pro Glu Ile Lys Asn 65 70 75 80 Ile Ala Thr Arg Ala Val Ala Glu Thr Gly Met Gly Asn Val Ala Asp 85 90 95 Lys Ile Leu Lys Asn Thr Leu Ala Ile Glu Lys Thr Pro Gly Val Glu 100 105 110 Asp Leu Tyr Thr Glu Val Ala Thr Gly Asp Asn Gly Met Thr Leu Tyr 115 120 125 Glu Leu Ser Pro Tyr Gly Val Ile Gly Ala Val Ala Pro Ser Thr Asn 130 135 140 Pro Thr Glu Thr Leu Ile Cys Asn Thr Ile Gly Met Leu Ala Ala Gly 145 150 155 160 Asn Ala Val Phe Tyr Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu 165 170 175 Trp Leu Ile Glu Lys Leu Asn Thr Ile Val Arg Glu Ser Cys Gly Ile 180 185 190 Asp Asn Leu Val Val Thr Val Glu Lys Pro Ser Ile Gln Ala Ala Gln 195 200 205 Glu Met Met Asn His Pro Lys Val Pro Leu Leu Val Ile Thr Gly Gly 210 215 220 Pro Gly Val Val Leu Gln Ala Met Gln Ser Gly Lys Lys Val Ile Gly 225 230 235 240 Ala Gly Ala Gly Asn Pro Pro Ser Ile Val Asp Glu Thr Ala Asn Ile 245 250 255 Glu Lys Ala Ala Ala Asp Ile Val Ala Gly Ala Ser Phe Asp His Asn 260 265 270 Ile Leu Cys Ile Ala Glu Lys Ser Val Val Ala Val Asp Ser Ile Thr 275 280 285 Asp Phe Leu Leu Phe Gln Met Glu Lys Asn Gly Ala Leu His Val Thr 290 295 300 Asn Pro Ser Asp Ile Arg Lys Leu Glu Lys Val Ala Val Thr Glu Lys 305 310 315 320 Gly Val Thr Asn Lys Lys Leu Val Gly Lys Ser Ala Ser Glu Ile Leu 325 330 335 Lys Glu Ala Gly Ile Ala Cys Asp Phe Thr Pro Arg Leu Ile Ile Val 340 345 350 Glu Thr Asp Arg Ser His Pro Phe Ala Thr Val Glu Leu Leu Met Pro 355 360 365 Ile Val Pro Val Val Arg Val Ala Asp Phe Asp Gln Ala Leu Glu Val 370 375 380 Ala Leu Glu Leu Glu Gln Gly Leu His His Thr Ala Thr Met His Ser 385 390 395 400 Gln Asn Ile Ser Arg Leu Asn Lys Ala Ala Arg Asp Met Gln Thr Ser 405 410 415 Ile Phe Val Lys Asn Gly Pro Ser Phe Ala Gly Leu Gly Phe Gly Gly 420 425 430 Glu Gly Ser Ala Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr 435 440 445 Thr Thr Ala Arg His Phe Ala Arg Arg Arg Arg Cys Val Leu Thr Asp 450 455 460 Gly Phe Ser Ile Arg 465 <210> 12 <211> 464 <212> PRT <213> Shewanella putrefaciens CN-32 <400> 12 Met Asn Thr Thr Glu Leu Glu Asn Met Ile Arg Asn Ile Leu Ala Asp 1 5 10 15 Asn Leu Lys Gly Thr Ala Thr Ala Pro Gly Asn Ile Gln His Thr Ile 20 25 30 Phe Ala Arg Val Glu Asp Ala Ile Thr Ala Ser Tyr Asp Ala Tyr Lys 35 40 45 Lys Tyr Met Ala Glu Pro Leu Ala Leu Arg Thr Arg Ile Ile Thr Ala 50 55 60 Leu Lys Glu Glu Leu Ala Pro Trp Ile Lys Glu Met Ser Glu Arg Ala 65 70 75 80 Ala Glu Glu Thr Gly Met Gly Asn Ala Pro Asp Lys Ile Ser Lys Asn 85 90 95 Thr Ala Ala Leu Asn Asn Thr Pro Gly Ile Glu Asp Leu Thr Thr Ser 100 105 110 Ala Leu Thr Gly Asp Gly Gly Met Val Leu Phe Glu Leu Ser Pro Phe 115 120 125 Gly Val Ile Gly Ala Ile Ala Pro Ser Thr Asn Pro Thr Glu Thr Ile 130 135 140 Ile Asn Asn Thr Ile Ser Met Leu Ala Ala Gly Asn Ala Val Tyr Phe 145 150 155 160 Ser Pro His Pro Gly Ala Lys Lys Val Ser Leu Trp Leu Ile Glu Lys 165 170 175 Ile Glu Asp Ile Ile Tyr Arg Val Ser Gly Ile Arg Asn Leu Val Thr 180 185 190 Thr Val Ala Glu Pro Thr Phe Asp Ala Thr Arg Glu Met Met Ser Asp 195 200 205 Pro Arg Ile Ala Leu Leu Ala Val Thr Gly Gly Pro Ala Ile Val Asn 210 215 220 Met Ala Met Lys Thr Gly Lys Lys Val Ile Gly Ala Gly Pro Gly Asn 225 230 235 240 Pro Pro Val Leu Val Asp Glu Thr Ala Cys Pro Val Lys Ala Ala Lys 245 250 255 Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn Val Leu Cys Ile Ala 260 265 270 Glu Lys Cys Val Ile Val Val Asp Ser Ile Ala Asp Arg Leu Met Asp 275 280 285 Asn Met Gln Lys Asn Asp Ala Phe Leu Val Lys Thr Pro Gly Asp Ile 290 295 300 Ala Arg Leu Arg Lys Val Val Ile Asn Asp Lys Gly Glu Ala Asn Lys 305 310 315 320 Lys Leu Val Gly Lys Ser Pro Ala Val Ile Leu Gln Ala Ala Asp Leu 325 330 335 Asn Thr Ser Thr Ala Pro Arg Leu Ile Ile Val Glu Val Glu Gln Asp 340 345 350 Asp Pro Leu Val Met Val Glu Gln Leu Met Pro Val Leu Pro Val Val 355 360 365 Arg Val Ser Asp Phe Glu Thr Gly Leu Ala Leu Ala Leu Lys Val Glu 370 375 380 Asn Glu Gln His His Thr Ala Ile Met His Ser Gln Asn Val Thr Arg 385 390 395 400 Leu Asn Leu Ala Ala Lys Thr Met Gln Thr Ser Ile Phe Val Lys Asn 405 410 415 Gly Pro Ser Tyr Ala Gly Leu Gly Ile Gly Ala Glu Gly Phe Thr Thr 420 425 430 Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Ser 435 440 445 Phe Ala Arg Lys Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 13 <211> 464 <212> PRT <213> Kosakonia radicincitans DSM 16656 <400> 13 Met Asn Thr Thr Glu Leu Glu Asn Met Ile Arg Thr Ile Leu Ala Asp 1 5 10 15 Asn Leu Thr Gly Ile Ala Thr Ala Pro Gly Asn Ile Gln His Thr Ile 20 25 30 Phe Ala Arg Val Glu Asp Ala Ile Thr Ala Ser Tyr Asp Ala Tyr Lys 35 40 45 Lys Tyr Leu Ala Glu Pro Leu Ala Leu Arg Thr Arg Ile Ile Thr Ala 50 55 60 Leu Lys Glu Glu Leu Ala Pro Trp Ile Lys Glu Met Ser Glu Arg Ala 65 70 75 80 Ala Glu Glu Thr Gly Met Gly Asn Ala Leu Asp Lys Ile Ser Lys Asn 85 90 95 Thr Ala Ala Leu Asn Asn Thr Pro Gly Ile Glu Asp Leu Thr Thr Ser 100 105 110 Ala Leu Thr Gly Asp Gly Gly Met Val Leu Phe Glu Leu Ser Pro Phe 115 120 125 Gly Val Ile Gly Ala Ile Ala Pro Ser Thr Asn Pro Thr Glu Thr Ile 130 135 140 Ile Asn Asn Thr Ile Ser Met Leu Ala Ala Gly Asn Ala Val Tyr Phe 145 150 155 160 Ser Pro His Pro Gly Ala Lys Lys Val Ser Leu Trp Leu Ile Glu Lys 165 170 175 Ile Glu Asp Ile Ile Tyr Arg Val Ser Gly Ile Arg Asn Leu Val Thr 180 185 190 Thr Val Ala Glu Pro Thr Phe Asp Ala Thr Arg Glu Met Met Ser Asp 195 200 205 Pro Arg Ile Ala Leu Leu Val Val Thr Gly Gly Pro Ala Ile Val Asn 210 215 220 Met Ala Met Lys Thr Gly Lys Lys Val Ile Gly Ala Gly Pro Gly Asn 225 230 235 240 Pro Pro Val Leu Val Asp Glu Thr Ala Cys Pro Val Lys Ala Ala Lys 245 250 255 Asp Ile Val Asp Gly Ala Ser Phe Asp His Asn Val Leu Cys Ile Ala 260 265 270 Glu Lys Cys Val Ile Val Val Asp Ser Ile Ala Asp Arg Leu Val Glu 275 280 285 Asn Met Gln Lys Asn Asp Ala Phe Leu Val Lys Thr Pro Gly Asp Ile 290 295 300 Ala Arg Leu Arg Gln Val Val Ile Asn Asp Lys Gly Glu Ala Asn Lys 305 310 315 320 Lys Leu Val Gly Lys Ser Pro Ala Val Ile Leu Gln Ala Ala Asp Leu 325 330 335 Asn Thr Ser Thr Ala Pro Arg Leu Ile Ile Val Glu Val Glu Gln Asp 340 345 350 Asp Pro Leu Val Met Val Glu Gln Leu Met Pro Val Leu Pro Val Val 355 360 365 Arg Val Arg Asp Phe Glu Thr Gly Leu Ala Leu Ala Leu Lys Val Glu 370 375 380 Asn Asp Gln His His Thr Ala Ile Met His Ser Gln Asn Val Ser Arg 385 390 395 400 Leu Asn Leu Ala Ala Lys Thr Met Gln Thr Ser Ile Phe Val Lys Asn 405 410 415 Gly Pro Ser Tyr Ala Gly Leu Gly Ile Glu Ala Glu Gly Phe Thr Thr 420 425 430 Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Ser 435 440 445 Phe Ala Arg Lys Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 14 <211> 467 <212> PRT <213> Tolumonas auensis DSM 9187 <400> 14 Met Asn Asn Thr Glu Leu Glu Ser Leu Ile Arg Thr Ile Leu Thr Glu 1 5 10 15 Gln Leu Thr Pro Ser Ala Thr Asp Thr Pro Ala Cys Thr Ala Ser Ser 20 25 30 Val Ala Leu Phe Asp Asp Val Asp Ser Ala Ile Cys Ala Ala His Ala 35 40 45 Ala Phe Leu Arg Tyr Gln Glu Ala Pro Leu Lys Thr Arg Ser Ala Ile 50 55 60 Ile Ala Ala Ile Arg Ala Glu Ile Ala Pro Cys Leu Ser Glu Leu Ala 65 70 75 80 Glu Arg Ala Ala Ala Glu Thr Gly Met Gly Asn Thr Ala Asp Lys Ile 85 90 95 Leu Lys Asn Lys Ala Ala Leu Glu Asn Thr Pro Gly Ile Glu Asp Leu 100 105 110 Lys Thr Thr Ala Leu Thr Gly Asp Glu Gly Met Val Leu Phe Glu Tyr 115 120 125 Ser Pro Phe Gly Val Val Gly Ala Val Ala Pro Ser Thr Asn Pro Thr 130 135 140 Glu Thr Ile Ile Asn Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ala 145 150 155 160 Ile Tyr Phe Ser Pro His Pro Gly Ala Lys Asn Ile Ser Leu Trp Leu 165 170 175 Ile Gln Lys Met Glu Glu Ile Ala Phe Lys Val Cys Gly Ile His Asn 180 185 190 Leu Ile Val Thr Val Lys Glu Pro Thr Phe Glu Ala Thr Gln Gln Met 195 200 205 Met Ala His Asp Lys Ile Ala Leu Leu Ala Ile Thr Gly Gly Pro Gly 210 215 220 Ile Val Asn Met Gly Leu Lys Ser Gly Lys Lys Val Ile Gly Ala Gly 225 230 235 240 Ala Gly Asn Pro Pro Cys Leu Val Asp Glu Thr Ala Glu Ile Val Lys 245 250 255 Ala Ala Gln Asp Ile Val Ala Gly Ala Ser Phe Asp Tyr Asn Leu Pro 260 265 270 Cys Ile Ala Glu Lys Ser Val Ile Ala Val Asp Cys Ile Ala Asp Gln 275 280 285 Leu Ile Gln Gln Met Arg Glu Phe Gly Ala Met Gln Ile Thr Asp Pro 290 295 300 Gln Gln Ile Ala Gln Leu Arg Glu Val Cys Ile Gln Lys Gly Ala Ala 305 310 315 320 Asn Lys Ser Leu Val Gly Lys Ser Pro Ala Thr Ile Leu Ala Ala Ala 325 330 335 Gly Ile Pro Cys Pro Ala Lys Glu Pro Arg Leu Ile Ile Leu Glu Val 340 345 350 Pro Ala Asn Asp Pro Phe Val Val Thr Glu Gln Leu Met Pro Val Leu 355 360 365 Pro Ile Val Arg Val Asp Asn Phe Glu Gln Gly Leu Gln Leu Ala Leu 370 375 380 Lys Val Glu Asp Gly Leu His His Thr Ala Met Met His Ser Gln Asn 385 390 395 400 Val Ser Arg Leu Asn Lys Ala Ala His Leu Met Gln Thr Ser Ile Phe 405 410 415 Val Lys Asn Gly Pro Ser Tyr Ala Gly Ile Gly Val Gly Ala Glu Gly 420 425 430 Phe Thr Thr Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser 435 440 445 Ala Arg Thr Phe Gly Arg Leu Arg Arg Cys Val Leu Thr Asn Gly Phe 450 455 460 Ser Ile Arg 465 <210> 15 <211> 461 <212> PRT <213> Citrobacter koseri ATCC BAA-895 <400> 15 Met Asn Thr Ser Glu Leu Glu Thr Leu Ile Arg Asn Ile Leu Ser Glu 1 5 10 15 Gln Leu Ala Pro Ala Gln Ala Glu Thr Gln Gly His Gly Ile Phe Gln 20 25 30 Ser Val Gly Glu Ala Ile Asp Ala Ala His Gln Ala Phe Leu Arg Tyr 35 40 45 Gln Gln Cys Pro Leu Lys Thr Arg Ser Ala Ile Ile Ser Ala Leu Arg 50 55 60 Gln Glu Leu Thr Pro His Leu Ala Thr Leu Ala Ala Glu Ser Ala Ala 65 70 75 80 Glu Thr Gly Met Gly Asn Lys Glu Asp Lys Phe Leu Lys Asn Lys Ala 85 90 95 Ala Leu Asp Asn Thr Pro Gly Ile Glu Asp Leu Thr Thr Thr Ala Leu 100 105 110 Thr Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro Phe Gly Val 115 120 125 Ile Gly Ser Val Ala Pro Ser Thr Asn Pro Thr Glu Thr Ile Ile Asn 130 135 140 Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ser Val Tyr Phe Ser Pro 145 150 155 160 His Pro Gly Ala Lys Asn Val Ser Leu Lys Leu Ile Gly Met Ile Glu 165 170 175 Asp Ile Ala Phe Arg Cys Cys Gly Ile Arg Asn Leu Val Val Thr Val 180 185 190 Ala Glu Pro Thr Phe Glu Ala Thr Gln Gln Met Met Ala His Pro Asn 195 200 205 Ile Ala Val Leu Ala Ile Thr Gly Gly Pro Gly Ile Val Ala Met Gly 210 215 220 Met Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly Asn Pro Pro 225 230 235 240 Cys Ile Val Asp Glu Thr Ala Asp Ile Val Lys Ala Ala Glu Asp Ile 245 250 255 Ile Asn Gly Ala Ala Phe Asp Tyr Asn Leu Pro Cys Ile Ala Glu Lys 260 265 270 Ser Leu Ile Val Val Glu Ser Val Ala Glu Arg Leu Val Gln Gln Met 275 280 285 Gln Ala Phe Gly Ala Leu Leu Leu Asn Ala Ala Asp Ile Asp Lys Leu 290 295 300 Arg Ala Val Cys Leu Pro Glu Gly His Ala Asn Lys Lys Leu Val Gly 305 310 315 320 Lys Ser Pro Ala Ala Met Leu Glu Ala Ala Gly Ile Ala Val Pro Ala 325 330 335 Lys Pro Pro Arg Leu Leu Ile Gly Ile Val Ser Ala Asp Asp Pro Trp 340 345 350 Val Thr Ser Glu Gln Leu Met Pro Met Leu Pro Val Val Lys Val Asp 355 360 365 Asn Phe Asp Ser Ala Leu Ala Leu Ala Leu Lys Val Glu Glu Gly Leu 370 375 380 His His Thr Ala Ile Met His Ser Gln Asn Val Ser Arg Leu Asn Leu 385 390 395 400 Ala Ala Arg Thr Leu Gln Thr Ser Ile Phe Val Lys Asn Gly Pro Ser 405 410 415 Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr Phe Thr Ile 420 425 430 Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr Phe Ala Arg 435 440 445 Ser Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 16 <211> 462 <212> PRT <213> Yersinia enterocolitica subsp. enterocolitica 8081 <400> 16 Met Asn Thr Asn Asp Leu Glu Ser Leu Ile Arg Thr Ile Leu Thr Glu 1 5 10 15 Gln Leu Thr Pro Val Thr Ala Pro Ala Ser Ser Ala Ile Phe Ala Ser 20 25 30 Val Asp Glu Ala Ile Asn Ala Ala His Ser Ala Phe Leu Arg Tyr Gln 35 40 45 Gln Ser Pro Met Lys Thr Arg Ser Ala Ile Ile Arg Ala Ile Arg Glu 50 55 60 Gln Leu Lys Pro Gln Leu Val Ser Leu Ser Glu Arg Gly Ala Ser Glu 65 70 75 80 Thr Gly Met Gly Asn Lys Glu Asp Lys Phe Leu Lys Asn Lys Ala Ala 85 90 95 Leu Glu Asn Thr Pro Gly Ile Glu Asp Leu Ser Thr Thr Ala Leu Thr 100 105 110 Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro Phe Gly Val Ile 115 120 125 Gly Ser Val Thr Pro Ser Thr Asn Pro Thr Glu Thr Ile Ile Asn Asn 130 135 140 Ser Ile Ser Met Leu Ala Ala Gly Asn Ala Val Tyr Phe Ser Pro His 145 150 155 160 Pro Gly Ala Lys Ala Val Ser Leu Asp Leu Ile Ala Gln Ile Glu Glu 165 170 175 Ile Ile Phe Asn Ser Cys Gly Ile Arg Asn Leu Val Val Thr Val Lys 180 185 190 Glu Pro Ser Phe Glu Ala Thr Gln Gln Met Met Ala His Asp Lys Ile 195 200 205 Ala Leu Leu Ala Ile Thr Gly Gly Pro Ala Ile Val Ala Met Ser Met 210 215 220 Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly Asn Pro Pro Cys 225 230 235 240 Leu Val Asp Glu Thr Ala Glu Leu Val Lys Ala Ala Gln Asp Ile Val 245 250 255 Ala Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys Ile Ala Glu Lys Ser 260 265 270 Leu Ile Val Val Glu Ser Val Ala Asp Arg Leu Leu Gln Gln Met Gln 275 280 285 Ala Phe Asp Ala Leu Leu Ile Ser Asn Pro Gln Glu Ile Asp Ser Leu 290 295 300 Arg Lys Ala Cys Leu Thr Pro Gln Gly His Ala Asn Lys Asn Leu Val 305 310 315 320 Gly Lys Ser Pro Ile Glu Leu Leu Lys Ala Ala Gly Ile Thr Cys Pro 325 330 335 Ala Lys Ala Pro Arg Leu Leu Leu Val Glu Val Ala Gly Asp Asp Pro 340 345 350 Leu Val Thr Thr Glu Gln Leu Met Pro Leu Leu Pro Val Val Arg Val 355 360 365 Lys Asp Phe Asp Ala Ala Leu Thr Leu Ala Leu His Val Glu Gly Gly 370 375 380 Leu His His Thr Ala Thr Met His Ser Gln Asn Val Ser Arg Leu Asn 385 390 395 400 Leu Ala Ala Arg Leu Leu Gln Thr Ser Ile Phe Val Lys Asn Gly Pro 405 410 415 Ser Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr Phe Thr 420 425 430 Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr Phe Ala 435 440 445 Arg Gln Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 17 <211> 464 <212> PRT <213> Salmonella enterica subsp. enterica serovar Mbandaka str. ATCC 51958 <400> 17 Met Asn Thr Ser Glu Leu Glu Thr Leu Ile Arg Thr Ile Leu Ser Glu 1 5 10 15 Gln Leu Thr Thr Pro Ala Gln Thr Thr Ala Gln Pro Gln Gly Lys Gly 20 25 30 Ile Phe Gln Ser Val Ser Glu Ala Ile Asp Ala Ala His Gln Ala Phe 35 40 45 Leu Arg Tyr Gln Gln Cys Pro Leu Lys Thr Arg Ser Ala Ile Ile Ser 50 55 60 Ala Met Arg Gln Glu Leu Thr Pro Leu Leu Ala Thr Leu Ala Glu Glu 65 70 75 80 Ser Ala Asn Glu Thr Gly Met Gly Asn Lys Glu Asp Lys Leu Leu Lys 85 90 95 Asn Lys Ala Ala Leu Asp Asn Thr Pro Gly Val Glu Asp Leu Thr Thr 100 105 110 Thr Ala Leu Thr Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro 115 120 125 Phe Gly Val Ile Gly Ser Val Ala Pro Ser Thr Asn Pro Thr Glu Thr 130 135 140 Ile Ile Asn Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ser Val Tyr 145 150 155 160 Phe Ser Pro His Pro Gly Ala Lys Lys Val Ser Leu Lys Leu Ile Ser 165 170 175 Leu Ile Glu Glu Ile Ala Phe Arg Cys Cys Gly Ile Arg Asn Leu Val 180 185 190 Val Thr Val Ala Glu Pro Thr Phe Glu Ala Thr Gln Gln Met Met Ala 195 200 205 His Pro Arg Ile Ala Val Leu Ala Ile Thr Gly Gly Pro Gly Ile Val 210 215 220 Ala Met Gly Met Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly 225 230 235 240 Asn Pro Pro Cys Ile Val Asp Glu Thr Ala Asp Leu Val Lys Ala Ala 245 250 255 Glu Asp Ile Ile Asn Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys Ile 260 265 270 Ala Glu Lys Ser Leu Ile Val Val Glu Ser Val Ala Glu Arg Leu Val 275 280 285 Gln Gln Met Gln Thr Phe Gly Ala Leu Leu Leu Ser Pro Ala Asp Thr 290 295 300 Asp Lys Leu Arg Ala Val Cys Leu Pro Glu Gly Gln Ala Asn Lys Lys 305 310 315 320 Leu Val Gly Lys Ser Pro Ser Ala Met Leu Glu Ala Ala Gly Ile Ala 325 330 335 Val Pro Ala Lys Ala Pro Arg Leu Leu Ile Ala Leu Val Ser Ala Asp 340 345 350 Asp Pro Trp Val Thr Ser Glu Gln Leu Met Pro Met Leu Pro Val Val 355 360 365 Lys Val Ser Asp Phe Asp Ser Ala Leu Ala Leu Ala Leu Lys Val Glu 370 375 380 Glu Gly Leu His His Thr Ala Ile Met His Ser Gln Asn Val Ser Arg 385 390 395 400 Leu Asn Leu Ala Ala Arg Thr Leu Gln Thr Ser Ile Phe Val Lys Asn 405 410 415 Gly Pro Ser Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr 420 425 430 Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr 435 440 445 Phe Ala Arg Ser Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 18 <211> 462 <212> PRT <213> Yersinia mollaretii ATCC 43969 <400> 18 Met Asn Thr His Asp Ile Glu Ser Leu Ile Arg Thr Ile Leu Thr Glu 1 5 10 15 Gln Leu Thr Pro Ala Thr Ala Ser Ala Val Ser Ala Ile Phe Ala Ser 20 25 30 Val Asp Glu Ala Val Thr Ala Ala His Ser Ala Phe Leu Arg Tyr Gln 35 40 45 Gln Ser Pro Met Lys Thr Arg Ser Ala Ile Ile Ser Ala Leu Arg Glu 50 55 60 Gln Leu Ala Pro Gln Leu Ala Ser Leu Ser Glu Arg Gly Ala Ser Glu 65 70 75 80 Thr Gly Met Gly Asn Lys Glu Asp Lys Phe Leu Lys Asn Arg Ala Ala 85 90 95 Leu Glu Asn Thr Pro Gly Ile Glu Asp Leu Ser Thr Thr Ala Leu Thr 100 105 110 Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro Phe Gly Val Ile 115 120 125 Gly Ser Val Ala Pro Ser Thr Asn Pro Thr Glu Thr Ile Ile Asn Asn 130 135 140 Ser Ile Ser Met Leu Ala Ala Gly Asn Ala Val Tyr Phe Ser Pro His 145 150 155 160 Pro Gly Ala Lys Ala Val Ser Leu Asp Leu Ile Ala Gln Ile Glu Ala 165 170 175 Ile Ile Phe Asn Arg Cys Gly Ile Arg Asn Leu Val Val Thr Val Gln 180 185 190 Glu Pro Ser Phe Glu Ala Thr Gln Gln Met Met Ala His Asp Lys Ile 195 200 205 Ala Leu Leu Ala Ile Thr Gly Gly Pro Ala Ile Val Ala Met Gly Met 210 215 220 Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly Asn Pro Pro Cys 225 230 235 240 Leu Val Asp Glu Thr Ala Glu Leu Val Lys Ala Ala Gln Asp Ile Val 245 250 255 Ser Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys Ile Ala Glu Lys Ser 260 265 270 Leu Ile Val Val Glu Ser Val Ala Asp Arg Leu Leu Gln Gln Met Gln 275 280 285 Ala Phe Asp Ala Leu Leu Ile Thr Gln Pro Gln Glu Val Asp Ser Leu 290 295 300 Arg Lys Ala Cys Leu Thr Pro Gln Gly His Ala Asn Lys Asn Leu Val 305 310 315 320 Gly Lys Ser Pro Ala Glu Leu Leu Lys Ala Ala Gly Ile Thr Cys Pro 325 330 335 Ala Lys Ala Pro Arg Leu Leu Leu Val Glu Val Ala Gly Asp Asp Pro 340 345 350 Leu Val Thr Thr Glu Gln Leu Met Pro Leu Leu Pro Val Val Arg Val 355 360 365 Lys Asp Phe Asp Ala Ala Leu Thr Leu Ala Leu Gln Val Glu Gly Gly 370 375 380 Leu His His Thr Ala Thr Met His Ser Gln Asn Val Ser Arg Leu Asn 385 390 395 400 Leu Ala Ala Arg Leu Leu Gln Thr Ser Ile Phe Val Lys Asn Gly Pro 405 410 415 Ser Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr Phe Thr 420 425 430 Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr Phe Ala 435 440 445 Arg Gln Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 19 <211> 460 <212> PRT <213> Escherichia fergusonii ATCC 35469 <400> 19 Met Asn Thr Arg Glu Leu Glu Asn Ile Ile Arg Asn Ile Leu Arg Glu 1 5 10 15 Gln Leu Ser Thr Thr Ala Asp Ala Pro Thr Asn Gly Ile Phe Asp Ser 20 25 30 Val Asp Glu Ala Ile Asn Ala Ala His Gln Ala Phe Leu Arg Tyr Gln 35 40 45 Gln Cys Pro Leu Lys Thr Arg Ser Ala Ile Ile Ser Ala Ile Arg Gln 50 55 60 Glu Leu Thr Pro His Leu Asp Met Leu Ala Thr Glu Ser Ala Asn Glu 65 70 75 80 Thr Gly Met Gly Asn Lys Glu Asp Lys Phe Leu Lys Asn Lys Ala Ala 85 90 95 Leu Asp Asn Thr Pro Gly Ile Glu Asp Leu Thr Thr Thr Ala Leu Thr 100 105 110 Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro Phe Gly Val Ile 115 120 125 Gly Ser Val Thr Pro Ser Thr Asn Pro Thr Glu Thr Ile Ile Asn Asn 130 135 140 Ser Ile Ser Met Leu Ala Ala Gly Asn Ser Val Tyr Phe Ser Pro His 145 150 155 160 Pro Gly Ala Lys Asn Ile Ser Leu Lys Leu Ile Ala Met Ile Glu Glu 165 170 175 Ile Ala Phe Arg Cys Ser Gly Ile His Asn Leu Ile Val Thr Val Ala 180 185 190 Glu Pro Thr Phe Glu Ala Thr Gln Gln Met Met Thr His Pro Asn Ile 195 200 205 Ala Val Leu Ala Ile Thr Gly Gly Pro Gly Ile Val Ala Met Gly Met 210 215 220 Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly Asn Pro Pro Cys 225 230 235 240 Ile Val Asp Glu Thr Ala Asp Leu Val Lys Ala Ala Glu Asp Ile Ile 245 250 255 Asn Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys Ile Ala Glu Lys Ser 260 265 270 Leu Ile Val Val Glu Glu Ile Ala Gly Thr Leu Val Gln Gln Met Gln 275 280 285 Asn Phe Gly Ala Leu Leu Leu Asn Lys Glu Glu Thr Asp Lys Leu Arg 290 295 300 Asp Val Cys Leu Pro Gln Gly Met Ala Asn Lys Gln Leu Val Gly Lys 305 310 315 320 Ser Pro Ala Ala Leu Leu Gln Ala Ala Gly Ile Ala Val Pro Leu Lys 325 330 335 Thr Pro Arg Leu Leu Ile Ala Leu Val Asp Ala Cys Asp Lys Trp Val 340 345 350 Thr Ser Glu Gln Leu Met Pro Met Leu Pro Ile Val Lys Val Lys Asp 355 360 365 Phe Asp Ser Ala Leu Thr Leu Ala Leu Lys Val Glu Glu Gly Leu His 370 375 380 His Thr Ala Ile Met His Ser Gln Asn Val Ser Arg Leu Asn Leu Ala 385 390 395 400 Ala Arg Thr Leu Gln Thr Ser Ile Phe Val Lys Asn Gly Pro Ser Tyr 405 410 415 Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr Phe Thr Ile Ala 420 425 430 Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Lys Thr Phe Ala Arg Ser 435 440 445 Arg Arg Cys Val Leu Thr Ser Gly Phe Ser Ile Arg 450 455 460 <210> 20 <211> 464 <212> PRT <213> Salmonella enterica subsp. enterica serovar Urbana str. ATCC 9261 <400> 20 Met Asn Thr Ser Glu Leu Glu Thr Leu Ile Arg Thr Ile Leu Ser Glu 1 5 10 15 Gln Leu Thr Thr Pro Ala Gln Thr Pro Ala Gln Pro Lys Gly Lys Gly 20 25 30 Ile Phe Gln Ser Val Ser Glu Ala Ile Asp Ala Ala His Gln Ala Phe 35 40 45 Leu Arg Tyr Gln Gln Cys Pro Leu Lys Thr Arg Ser Ala Ile Ile Ser 50 55 60 Ala Met Arg Gln Glu Leu Thr Pro Leu Leu Ala Thr Leu Ala Glu Glu 65 70 75 80 Ser Ala Asn Glu Thr Gly Met Gly Asn Lys Glu Asp Lys Leu Leu Lys 85 90 95 Asn Lys Ala Ala Leu Asp Asn Thr Pro Gly Val Glu Asp Leu Thr Thr 100 105 110 Thr Ala Leu Thr Gly Asp Gly Gly Met Val Leu Phe Glu Tyr Ser Pro 115 120 125 Phe Gly Val Ile Gly Ser Val Ala Pro Ser Thr Asn Pro Thr Glu Thr 130 135 140 Ile Ile Asn Asn Ser Ile Ser Met Leu Ala Ala Gly Asn Ser Ile Tyr 145 150 155 160 Phe Ser Pro His Pro Gly Ala Lys Lys Val Ser Leu Lys Leu Ile Ser 165 170 175 Leu Ile Glu Glu Ile Ala Phe Arg Cys Cys Gly Ile Arg Asn Leu Val 180 185 190 Val Thr Val Ala Glu Pro Thr Phe Glu Ala Thr Gln Gln Met Met Ala 195 200 205 His Pro Arg Ile Ala Val Leu Ala Ile Thr Gly Gly Pro Gly Ile Val 210 215 220 Ala Met Gly Met Lys Ser Gly Lys Lys Val Ile Gly Ala Gly Ala Gly 225 230 235 240 Asn Pro Pro Cys Ile Val Asp Glu Thr Ala Asp Leu Val Lys Ala Ala 245 250 255 Glu Asp Ile Ile Asn Gly Ala Ser Phe Asp Tyr Asn Leu Pro Cys Ile 260 265 270 Ala Glu Lys Ser Leu Ile Val Val Glu Ser Val Ala Glu Arg Leu Val 275 280 285 Gln Gln Met Gln Thr Phe Gly Ala Leu Leu Leu Ser Pro Ala Asp Thr 290 295 300 Asp Lys Leu Arg Ala Val Cys Leu Pro Glu Gly Gln Ala Asn Lys Lys 305 310 315 320 Leu Val Gly Lys Ser Pro Ser Ala Met Leu Glu Ala Ala Gly Ile Ala 325 330 335 Val Pro Ala Lys Ala Pro Arg Leu Leu Ile Ala Leu Val Ser Ala Asp 340 345 350 Asp Pro Trp Val Thr Ser Glu Gln Leu Met Pro Met Leu Pro Val Val 355 360 365 Lys Val Ser Asp Phe Asp Ser Ala Leu Ala Leu Ala Leu Lys Val Glu 370 375 380 Glu Gly Leu His His Thr Ala Ile Met His Ser Gln Asn Val Ser Arg 385 390 395 400 Leu Asn Leu Ala Ala Arg Thr Leu Gln Thr Ser Ile Phe Val Lys Asn 405 410 415 Gly Pro Ser Tyr Ala Gly Ile Gly Val Gly Gly Glu Gly Phe Thr Thr 420 425 430 Phe Thr Ile Ala Thr Pro Thr Gly Glu Gly Thr Thr Ser Ala Arg Thr 435 440 445 Phe Ala Arg Ser Arg Arg Cys Val Leu Thr Asn Gly Phe Ser Ile Arg 450 455 460 <210> 21 <211> 1434 <212> DNA <213> Lactobacillus reuteri DSM 20016 <400> 21 ttaataccag ttacgtactg agaatccttg tggtgagttc aaacgaaccc gacgagtaaa 60 tgttcgtgca gtacatgttc cttcaccagt tggcgtagca attgttaatg ctgaagcacc 120 tgagtgagct ccattatctg caacacctgt accaacatag gatgggccat taacaacaaa 180 gattgaacat tgcatccggt gagcagcatt attaatatgc ttaaggttat tggaatgaat 240 agtagctgtg tgatggttac ctttttcaac ttcaacagca gtcttcaaaa catcatcaaa 300 tgttggacaa gaaacaactg gtaaaattgg cattaacatt tcagtcatta ctaatggatg 360 ttccttagga agttcacaaa taatttcaac tgggtggcct gtgtaaggaa tattagcttg 420 gtctaagata taagttgcat ccttaccaac aaatttacga tcaggagcac cattttcttg 480 gatacacata tcagctaatt tatcggcttg ttcacggtta actacaaagg caccttcatc 540 ttgcatctta cgaattaatt catctttaat gctactttct gcaactactt ccttttcagc 600 agtacataaa atatcattat caaatgaagc agatgtaatg atattatgag cagctaaatc 660 aatatcagca gtagcatcaa ccattgcagg aggattacca ggaccagcac caaccgcttt 720 cttaccactg gtcattgctt ggtgaacaac agctgggcca ccagttactg ctaacattgc 780 aatgtcgggg tgcttcatca tttgttgaac tgattcaatt gttggtgttt caatacttac 840 aactaaatta tgaaggcctg ttgcatctgc aataaaatcg ttcatctttt caattgtcca 900 gcgagtaaca ttctttgcgc cagggtgagc accaaagtaa agagtattac caccggcaag 960 catcatgatc gcattagcaa ttacagtttc tgaagggttt gtacttgggc caaccgcacc 1020 aataacacca tatggtaacc gttcatacat aaccatccca ccgtcaccgt tttctacaac 1080 tggttcaaga atctcgggac caggagtgtt gtacaaggca ttgtttaact tagcaatttt 1140 ggcctctact gttcccattc ctgtttcttc tttgatatct ttagccattt tttcaatata 1200 tgggcggaat ccttccttaa tggcatcaat cacttgttgg cgaacagcaa ttggcttatc 1260 ccgatatatt tcttgagcag cttttgcagc agcaattgca tcattgacat tagtgaaaat 1320 tccgcgatga ccattatcag tagtagctgc aacgtttgca cttgaagagc tggcattatc 1380 tagttcttcg gcaagaattt tgcgtacagc actttcaata tcattaatct gcat 1434 <210> 22 <211> 1434 <212> DNA <213> Lactobacillus brevis ATCC 367 <400> 22 ctaagcctcc caagtccgta atgagaaccc ttctggcgag ttaagccggc gccggcgcgt 60 gtaagtctta gacgttgccg ttccttcacc cgttggtgtg gcaatcgtta aggctgaagc 120 cccactatgc gcaccattcg ttgcaacacc agtcccacaa taagttgggc cgttaaccac 180 gaagattgac gtattcaacc ggtgcgctgc cttattgatg tgtggtaaat tctcggaatg 240 aatggaagcc gtgtggtgta acccaccttc aacttctgta gccgttgcta aaacgctatc 300 aaagtcaggg caacaaacga ctggcaaaat tggcatcaac atttctgtcg ttactaacgg 360 atgatcctta gctgcttcaa gaataatcag tgttggtgtc ccggtgtaag agattcctgc 420 ttgatccaaa atgtaagtgg catctttacc aacaaacttc cgatctggtg cgcccttagg 480 cccaatggtc atttgcgcta atttttcaat atcggcagaa tcggtaacca agaaggcccc 540 ttcttgttgc atccgcatga tgagttcatc cttgacagcg gcttcaacga caacttcctt 600 ttcggccgtg cagagaatgt tattatcaaa ggctgctgaa tcaacaatgt tgtgggctgc 660 taaagcaata ttggcagttg catccaccat tgccggtggg ttaccagcac cggcaccaac 720 cgccttctta ccactgataa gcgcttggtg gacaacagca ggccctccag tgattgacag 780 catggcaacg tcaggatgtt gcataacttc ttgcacggat tcaattgaag gcgtttccag 840 tgaaacgact aagttatgta acccagttgc atcagctacc aattcgttta atttttcgat 900 cgtccaacgg gtaatgttct tagcacctgg atgggcacca aagaacaacg tattcccacc 960 agccaacatc atgatggcat tggcaatcac cgtttcagag gggttggtac taggaccaac 1020 ggcaccaatg acaccaaatg gcgcgtattc atacataacc agtccaccgt caccggtttc 1080 ggcttctggc tgcagaattt ctggaccggg tgtgttataa agggcgttat tgagtttggc 1140 aattttcgcg ctaaccgttc ccatgccagt ttcgtcatga atccgcttag ccatatcctc 1200 aatgtatggc cggaaaccct cacggatcgc atcaatcact ttgttccgaa ctgagattgg 1260 ttggtcggcg tagttttctt gcgccgcttt tgtggccgca atggcttcat tgaccgtctt 1320 aaagatccca tttttgccgg gaacggtcgt attggtggcc gttgatgact gaggattgct 1380 aagttcttca ctcaaaattt tacggatggc ttgttcaatg ttttctgtgt tcat 1434 <210> 23 <211> 1434 <212> DNA <213> Pediococcus acidilactici <400> 23 ttatgcctcc cacgaacgta acgaaaatgc ttctggcgaa tttaaccgac gacggcgagt 60 gaaggtctta gctgttgcgg ttccttctcc agttggggtt gcaatcgtta atgctgaagc 120 acctgcatga gcaccgttag ctcctacccc ggttcccaca tacgttgcac cgttaactac 180 gaaaatggaa gtgttcattc ggtgtgccgc acggttaata tttggtaagt tttcagaatg 240 aattgaagcc gtgtgatgca atccttgttc aacttctacc gcagtcgcta aaacttgatc 300 aaacgttggg caagaaacta ctggtacaat tggcatcaac atttcggtag taaccaatgg 360 atgatccttt tgggcttcca aaataattaa ttttggtgtg ccagtgtatg caattcctgc 420 cttatctaaa atgtaggttg catctttacc gacgaattgg cggtcaggtg caccattttt 480 gccaatggtc atttctgcta acttatcgat atcactagcg ttagttacca aaaatgctcc 540 ttcttcttgc atctttttaa taagttcatc cttcacgcta gattcaacta ctacttcttt 600 ttctgcggta caaaggatgt tgttatcaaa agatgcggaa tctacaatgt tgtgtgcagc 660 taacgcaacg ttagcagtgg catcaacaat tgcaggaggg ttacctgcac cagcacccac 720 ggcttttttg ccactgacta atgcttggtg aaccactgct gggccgcccg tgatggacag 780 cattgcaatg tcaggatgtt gcatcatttc ttgaacagat tcaattgacg gttcttcaat 840 tgatacaact aaattcttca tcccggtagc ttcataaact aactcgttta acttttcaat 900 tgtccaacgg gtaatttttt tagcacctgg atgggcgccg aagtacaacg tatttccacc 960 agctaacatc ataatggcgt tagcaattac cgtttcagaa gggttagtac ttggaccaac 1020 tgccccaatt actccaaacg gagcgtattc gtacatcact aaaccaccgt cgccagtttc 1080 agcttcaggt tgcaaaattt cagttccagg agtgttatat aaagcattgt ttagcttagc 1140 aattttagct tcaaccgttc ccattcctgt ttcatcttta atgtccttag ccattttttc 1200 gatgtatgga cggaatcctt cacgaattgc tgttaaaacc ttttcccgaa aggctaatgt 1260 tttatcaatg taaacatctt cagctgcctt agctgccgcg attgcttcat ccacggtctt 1320 aaaaattccg ttttggcccg catcactagt tgacgcagtt tggctagtgc cacttttctt 1380 tagttcttca cttaaaatgc gtcgaatatc ttcttcaaga ttttgaattt ccat 1434 <210> 24 <211> 1431 <212> DNA <213> Pediococcus claussenii ATCC BAA-344 <400> 24 ttatgcctcc catgaacgaa gtgagaatgc ctcaggagaa tttagacgac gacgtcttgt 60 aaatgtttta gcagttgctg taccttcacc agtcggtgtg gcaattgtca aagctgatgc 120 tccagcatgc gcgccgtttg cacctactcc tgtaccaaca tacgttgcac cattaacaac 180 aaaaattgat gtattcatac ggtgtgctgc ttgattaata tgtggtaagt tctctgaatg 240 aattgaagct gtatgatgta aaccttgttc gacctcaaca gctgttgcta atgcacggtc 300 aaaggttggg caggaaacaa ctggtaagat tggcatcaac atttccgttg ttactaatgg 360 atgattctta tgagcctcaa gaataattag tttgggtgtt ccagtataat taattcctgc 420 actatcaaga ataaatgttg cgtctttacc aacaaattta cggtccggag caccattctt 480 accaatcgta atttgaacta ttttttcaat atcatgggca cttgtaacca agaatgcacc 540 ctcgttttgc attttagaaa ttagttcatc tttaacgctt tcctcaacaa caacttcttt 600 ttctgctgta cacaaaatat tgttatcaaa agatgatgaa tccacaatat tatgtgcggc 660 taaatcgata ttagcagttg catcaacaat tgcaggtggg ttaccagctc cagcaccaac 720 cgccttctta ccactaatca tagcttgatg aacaacacca gggcctccag taattgaaag 780 catcgcaata tcaggatgtt gcatcatctg ttgaactgat tcaattgaag gttcttcgat 840 tgaaacaacc atgtttctca taccagttgc cttaaaaaca aattcattta acttttcaat 900 tgtccatcgt gtgatttttt tagcaccagg atgcgcacca aagtacacag tgttaccacc 960 agcaagcatc attaaagcat ttgcaataac tgtttcagat gggtttgtac ttggtcctac 1020 agccccaatc actccaaatg gtgcatattc atataatact aatccgccat cacccgtctc 1080 agcttctggt tcgagtattt ctgttccagg agtattgtat agagcattgt tcaatttagc 1140 gatctttgct tcaacagtac ccattcctgt ttcttctttg atatctttag ccatttgttc 1200 aatataaggc cggaaacctt ctttaatggc atctaatact tggtttcgaa aagccaatga 1260 tttgtcaaca tatatttctt gcgcagcttt agcggcagca atcgcttcgt caactgtttt 1320 gaagattccg ttcgttccat tatcacttga agcgcttaca gaattatcac tatcttgtag 1380 ttcctcactt aaaattcggc ggatatcttg ttctaactta tccatttcca t 1431 <210> 25 <211> 1446 <212> DNA <213> Lactobacillus collinoides <400> 25 atggcagatc aaaatattga agcagaaatc agacgaattt tacaagaaga attaagcggt 60 aacgcttcgt ccagcgctgc tggtacgact accagtcaac ctgatgggtt aggcaaccgg 120 atcttcacca acgtgaacga tgccattgct gctgctaagc aagctcaggc aatctaccaa 180 gataaaccac ttgccttccg taaaaaagtc gttcaagcaa ttaaagatgg tttcggccca 240 tacattgaat atatggcaaa gcagacccgt gaagaaactg gcatgggaac tgccgaagct 300 aagattgcta agttaaagaa cgccctctac aacaccccag gcgttgaatt actggaccca 360 gaagttgaaa ctggtgacgg cgggatggtc atgtatgaat acacgccatt cggtgttatc 420 ggtgccgttg gaccaagtac aaacccttgt gaaacggttc tgaacaactc catcatgatg 480 atgtctgctg ggaacgcatt gttctttggc gcccatcctg gtgcaaagaa cattactcgc 540 tgggcagttg aaaaattgaa cgaattcgtt tacaaggcta ctgggttgaa gaacctctta 600 gtttccttgg acacaccatc aattgaatcc gttcaagaaa tgatgcaaca tccagatgtt 660 gcaatgctgg ctgtaactgg tggcccagct gttgtgcatc aagcattaac gagtggtaaa 720 aaagccgttg gtgccggtgc tggtaacccg cctgcaatgg ttgatgcaac tgctgatatt 780 gatttagcag ctcataacct atttacttca gctaagtttg acaatgaaat tctgtgtact 840 tcagaaaagg aaatcattgc tgaagattca attaaggatg aacttcttca aaagattgtt 900 gctaagggcg cttgcctagt aactgatcct aaagacatca agcatttagc tgacatgacc 960 attggggaca acggtgcccc tgaccggaaa tatgttggta aggatgccac tgttatctta 1020 gatgccgctg gtatttcata caccggcgat cctaagttga tcatgatgga tgttgataaa 1080 gacaacccat tggttaagac agaaatgttg atgccaatct tgcctatcgt tgggtgccca 1140 gactttgacg ccgttttggc tacggctatt gaagttgaag gtggcaatca ccatactgct 1200 tcaattcact cgaacaacat cctgcacatc aacaaggctg ctcaccggat gaacacctcg 1260 atcttcgtcg caaatggccc aacatttgcc gcaactggtg tcggtgataa cggttattac 1320 agtggtgctg ctgcgctgac aattgctacc ccaaccggtg aaggtactac cactactaag 1380 acctttaccc gtcgtcgtcg tttcaactgt ccacaagggt tctcacttcg ttcttgggag 1440 gtttaa 1446 <210> 26 <211> 1410 <212> DNA <213> Listeria welshimeri serovar 6b str. SLCC5334 <400> 26 atggaatcat tagaactcga acaactggtg aaaaaagttc tgttagaaaa attagctgaa 60 caaaaagatg taccagtaaa aacaactaca caaggcgcaa aaagtgggat ttttgataca 120 gtggatgagg cagttcaagc agctgtccaa gcacaaaata gttataaaga aaaatctctg 180 gaagaacgcc gcaatgtagt aaaagcaatt cgtgaagcac tttatccaga aattgagtca 240 attgccacaa gagcagttgc tgaaacagga atgggtaatg tgacagataa aattttgaaa 300 aatactttag cgattgaaaa aacgccgggc gtagaagatt tatatacaga agtagctact 360 ggtgataatg gcatgacgct ttatgaatta tctccgtatg gtgtaattgg tgctgtggcg 420 ccgagtacga atccaaccga aacgttaatt tgtaatacaa tcggtatgct tgcagctggg 480 aatgcagtgt tttatagccc acatcctggt gcaaaaaata tatctctttg gttgattgaa 540 aagttgaata cgattgttcg tgaaagttgt ggtattgata acttggttgt gacagtggaa 600 aaaccttcca ttcaagcagc gcaagaaatg atgaatcatc caaaagtacc attacttgtc 660 attacaggtg gaccaggcgt cgtgcttcaa gcaatgcaat caggtaaaaa agtcattgga 720 gctggtgccg gaaatccgcc ttccatcgta gacgaaacag ctaatatcga aaaagctgca 780 gccgatattg ttgacggagc ctcttttgac cacaatatct tatgtattgc tgaaaaaagc 840 gttgttgccg ttgatagcat tactgatttc ctattattcc aaatggaaaa aaatggagca 900 ctacatgtga ccaatccgag cgatattaaa aaattagaaa aagttgctgt aacggataaa 960 ggtgtaacga ataaaaaatt agtcggaaaa agcgcttctg aaattttaaa agaagctgga 1020 ataacttgtg attttacccc gcgattaatc attgtggaaa cagataaatc acatccattt 1080 gcaacagtag aattactaat gccaatcgtt ccagtggtaa gagtgcctga ttttgatgaa 1140 gcgcttaaag tagctattga attagaacaa ggactacatc atacagcaac aatgcattca 1200 caaaatattt ccagattaaa taaagctgca agagatatgc aaacatcgat ctttgtgaaa 1260 aatggtcctt cctttgcagg tttaggtttt agaggggaag gtagtactac atttactatt 1320 gcaaccccaa ctggagaagg aaccactaca gcacgtcatt ttgctagacg ccgccgttgt 1380 gttttaacag atggtttttc gattcgttaa 1410 <210> 27 <211> 1410 <212> DNA <213> Listeria innocua Clip11262 <400> 27 atggaatcat tagaactcga acaactggta aaaaaagttc tcttagaaaa attagcagaa 60 caaaaagaag taccaacaaa aacaactaca caaggcgcga aaagtggcgt ttttgataca 120 gttgacgagg ctgttcaagc agcagttata gcgcagaatt gctataaaga aaaatcactt 180 gaagaacgcc gcaatgttgt aaaagcaatt cgtgaagcac tttatccaga aattgaaaca 240 attgcgacaa gagcagttgc agagactggt atgggaaatg tgacagataa aattttgaaa 300 aacacgttag caatcgaaaa aacgccaggg gtagaagatt tatatacaga agtagctaca 360 ggtgataacg gtatgacact atatgaactc tctccgtatg gcgtaattgg tgcagtagcg 420 ccgagcacaa acccaacgga aacattgatt tgtaattcaa tcggtatgct cgcagctgga 480 aatgccgttt tttatagccc tcatccaggg gcaaaaaaca tttcactgtg gttgattgaa 540 aaactaaaca caattgttcg cgatagttgt ggtatagata atctaattgt caccgtggct 600 aaaccatcca tccaagcagc tcaagaaatg atgaaccatc caaaagtacc gctacttgtt 660 attacaggtg gtccgggcgt tgttctccaa gcgatgcaat caggtaaaaa agtgattgga 720 gcaggagcag ggaacccgcc ttctattgtt gacgaaacag ctaatatcga aaaagcggct 780 gctgacatcg tagacggagc atcttttgac cataatattt tatgtattgc tgaaaaaagt 840 gtggtagctg ttgatagcat tgctgatttc ttgttattcc aaatggaaaa aaatggtgcc 900 cttcatgtta ctaatccaag tgatattcaa aaattagaaa aagtagccgt taccgataaa 960 ggtgtaacta ataaaaaatt agtcggaaaa agtgcaactg aaatcttaaa agaagcagga 1020 atagcttgtg attttacacc acgtttaatc attgtggaaa cggagaaatc tcatccattt 1080 gcaacagtag agctattaat gccaatcgtt ccagttgtaa gggtgcctga ttttgacgaa 1140 gcccttgaag tggctattga actcgaacaa ggcttacatc atacagcaac aatgcattca 1200 caaaatatct cgagattaaa caaagctgca agagatatgc aaacttccat ctttgtcaaa 1260 aatggtccgt cctttgcggg attaggcttt agaggagaag gtagtactac tttcactatt 1320 gcaacgccta ctggagaagg aacaactaca gcacgtcatt ttgctagacg ccgccgctgt 1380 gttttaacag atggtttttc gattcgttaa 1410 <210> 28 <211> 1410 <212> DNA <213> Listeria monocytogenes ATCC 19117 <400> 28 atggaatcat tagaactcga acaactggta aaaaaagttc ttttagaaaa attagcagaa 60 caaaaagatg caccagtaaa aacaacggtc aaaggcgcga aaagtggggt ttttgataca 120 gttgacgagg ccgttcaagc agcagttata gcacaaaata actataaaga aaaatcatta 180 gaagaacgcc gcaacgttgt gaaagcaatt cgcgaagcac tttatccaga aattgaatcc 240 attgcagcgc gagcagttgc tgaaacaggt atgggaaatg tagcagataa aattttgaaa 300 aacacgttag cgattgaaaa aacgccaggt gtggaagatt tgtatacaga agttgctact 360 ggtgataatg gcatgacgct ttacgaactt tctccatatg gcgtaatcgg agctgttgca 420 ccaagcacga acccaacgga aaccttgatt tgcaatacaa tcggcatgct cgcagctggg 480 aatgcagtat tttatagccc gcatccaggt gcgaaaaata tttctctttg gttgattgaa 540 aagttgaata cgattgtccg tgaaagttgc ggcattgata atttagttgt tacagtcgaa 600 aaaccatcta ttcaagccgc gcaagaaatg atgaatcatc cgaaagtacc gctccttgtt 660 attacaggtg gccctggtgt agttcttcaa gccatgcaat ccggtaaaaa agttattggc 720 gcaggtgccg ggaatccgcc atctattgta gatgagacag caaacatcga aaaagcagct 780 gctgatatcg tagacggcgc atcttttgac cataatattc tatgtattgc ggagaaaagt 840 attgttgcag ttgatagcat cgcagatttc ttaatgttcc aaatggaaaa aaatggtgca 900 ctacatgtga ccaatccaag cgatattcaa aaactagaaa aagtagctgt cacagataaa 960 ggcgtaacaa acaaaaaact agtcggaaaa agtgcttcag aaattttaaa agaagcgggg 1020 attgtttgtg atttttcacc acgtttaatt attgtggaaa cagaaaaaac acatccgttt 1080 gcaactgtag aattattgat gccgattgtt cctgttgtaa gagttcctaa ttttgacgaa 1140 gcgcttgatg tcgctattga gttagagcaa ggcttgcatc acacagctac gatgcattca 1200 caaaatattt ctagattaaa caaagctgca cgagatatgc aaacatccat ctttgtcaaa 1260 aatggtcctt catttgcggg attaggcttt agaggagaag gtagcactac tttcactatt 1320 gcaacgccta ccggagaagg aaccactaca gcgcgccatt ttgctagacg tcgccgttgt 1380 gttttaacag atggtttttc gattcgttaa 1410 <210> 29 <211> 1410 <212> DNA <213> Listeria marthii FSL S4-120 <400> 29 atggaatcat tagaactcga acaactggtg aaaaaagttc ttttagaaaa attagcagaa 60 caaaaagaag caccagcaaa accaataaca caaggtgcga aaagtggtat ttttgatacc 120 gtcgatgaag ccgttcaagc agcagtaata gcgcaaaatt gttataaaga aaaatcacta 180 gaagaacgcc gcaatgttgt gaaagcaatt cgcgaaactc tttatccaga aattgaaaca 240 atcgcgacga aagcagtagc agaaacagga atgggtaatg tagcagataa aattttgaaa 300 aacactttag cgattgaaaa aactccaggg gtagaagatt tatatacaga agtagctact 360 ggcgataatg gtatgacact ttatgaacta tctccgtatg gcgttattgg tgcagttgcg 420 ccgagcacga atccgactga aacattgatt tgtaatacga tcggcatgct cgctgcggga 480 aatgcagtat tttacagtcc gcatccaggg gcaaaaaata tttctctatg gttgattgaa 540 aaactaaata caattgttcg cgaaagttgc ggaattgata atttggtcgt tacagtcgaa 600 aaaccatcta ttcaagctgc acaagaaatg atgaatcatc cgaaagtacc gttacttgtg 660 attacaggtg gcccaggcgt agttctgcaa gcgatgcaat ccggtaagaa agtgattggt 720 gctggagccg gaaatccgcc gtcaatcgta gacgaaacag ctaatattga aaaagctgcg 780 gctgatatcg tggacggagc atcttttgac cataatattt tatgtatcgc ggaaaaaagt 840 attgtggcag tagagagcat tgctgatttc ttattattcc aaatggaaaa aaatggtgca 900 ctgcatgtga ccaatccaag tgatattcaa aaattagaaa aagtggcagt aacagataaa 960 ggcgtgacca ataaaaaatt agttgggaaa agtgccgcag aaattttaaa agaagctggc 1020 ataacttgtg actttacccc gcgtttaatc attgtagaaa cgacaaaaac gcatccattt 1080 gcaacagtgg aactattaat gccaatcgtt ccgcttgtaa gagtgcctga ttttgacgaa 1140 gcacttgaag tagcaattga gttagagcaa ggattacatc atactgcaac gatgcattca 1200 caaaatattt ccagattaaa caaagcggca agagacatgc aaacatccat ctttgtaaaa 1260 aatgggcctt catttgcagg attaggtttc agaggtgaag gtagcactac gtttaccatt 1320 gcaacgccta ccggagaagg aaccactaca gcacgtcatt ttgctagacg ccgccgttgt 1380 gttttaactg atggtttttc gattcgttaa 1410 <210> 30 <211> 1410 <212> DNA <213> Listeria ivanovii subsp. ivanovii PAM 55 <400> 30 atggaatcat tagaactcga acaactggtg aaaaaagttc tcttagaaaa attagcagga 60 caaaacgaag aaacaccaaa aaaaccaagc caaggtgcca aaagtggcat ttttgacaca 120 gtggatgagg cagttcaagc agcagtaatt gcgcaaaact gctacaaaga aaagtcgcta 180 gaagaccgca gaaatgtagt aaaagcaatt cgcgaagcac tttatccgga aatcgaaaat 240 attgcgacac gtgcggctgc tgaaacaggt atgggtaatg tagccgataa aattttgaaa 300 aatacgttag caattgaaaa aacaccagga gtagaagatc tctatacaga agtagctact 360 ggcgataatg gtatgacgct ttatgaactt tctccttatg gtgttattgg tgctgttgct 420 ccaagtacga atccaacaga aacattaatt tgcaacacaa ttggaatgct tgcagctgga 480 aatgcagttt tttatagccc gcatccaggt gcaaaaaata tttcgctttg gttgattgaa 540 aaactaaata cgattgttcg tgaaagctgc ggaatcgata acctagtcgt tacagtagaa 600 aaaccatcta ttcaagcagc acaagaaatg atgaatcatc caaaagttcc gttactagtt 660 atcactggcg gccctggcgt tgttcttcaa gcgatgcaat ccggtaagaa agtaatcgga 720 gcaggcgctg gaaatccacc gtctatcgta gacgaaacag cgaatatcga aaaagcagct 780 gcagatatcg ttgcgggcgc atcttttgat cataatattt tatgtatcgc agaaaaaagc 840 gtagtagcag tggacagcat tactgatttt ctattattcc aaatggaaaa aaatggcgcc 900 tttcatgtta cgaatccaag cgatattcgc aaactggaaa aagtggcggt taccgaaaaa 960 ggcgttacca acaagaagtt agttggtaaa agcgcttcgg aaattttaaa agaagcaggg 1020 atagcatgtg attttacccc tcgattaatt attgctgaaa cagatagatc ccatccattt 1080 gcaacggtag aactgctaat gccaattgtt ccagttgtca gagtggctga ttttgatcaa 1140 gcacttgaag tagcacttga gttagaacaa ggcttgcatc atacggcaac aatgcattcg 1200 caaaatattt ctagactgaa caaagcagca agagatatgc aaacttctat ttttgtgaaa 1260 aatggaccat cgtttgctgg acttggcttt ggaggagaag gtagtgcgac tttcactatc 1320 gctaccccaa caggtgaagg aactactaca gcgcgacact ttgctagacg ccgtcgttgt 1380 gttttaacag atggtttttc gattcgttaa 1410 <210> 31 <211> 1410 <212> DNA <213> Listeria seeligeri serovar 1/2b str. SLCC3954 <400> 31 atggaatcat tagaactcga acaactggtg aaaaaagttc tcttagaaaa attagcagga 60 caaaacgaag aaacaccaaa aaaaccaagc caaggtgcca aaagtggcat tttcgataca 120 gtggatgagg cagttcaagc agcagtaatt gcgcaaaact gctacaaaga gaagtcacta 180 gaagaccgca gaaatgttgt aaaagcaatt cgtgaagcac tttatccgga aatcaaaaat 240 attgcgacac gtgcggttgc tgaaacaggt atgggtaacg tagccgataa aattttgaaa 300 aatacgttag caattgaaaa aacaccagga gtagaagatc tctatacaga agtagctaca 360 ggcgataatg gtatgacgct ttatgaactt tctccttatg gtgttattgg tgctgttgct 420 ccaagtacga atccaacaga aacattaatt tgcaacacaa ttggaatgct tgcagctgga 480 aatgcagttt tttatagccc gcatccaggt gcaaaaaata tttcgctttg gttgattgaa 540 aaactaaata cgattgttcg cgaaagctgc gggattgata acctagtcgt tacagttgaa 600 aaaccatcta ttcaagcagc gcaagaaatg atgaatcatc caaaagtacc gttactagtt 660 atcactggcg gtcctggtgt tgttcttcaa gcgatgcaat ctggtaagaa agtaatcgga 720 gcaggtgcgg gaaatccacc ttctatcgta gacgaaacag cgaatatcga aaaagcagct 780 gctgatatcg ttgcgggtgc atcttttgat cataatattt tatgtatcgc agaaaaaagc 840 gtagtagcag tggatagcat cactgatttt ctcttattcc aaatggaaaa aaatggtgcg 900 ttgcatgtta cgaatccaag cgatattcgc aaactggaaa aagtggcagt taccgaaaaa 960 ggcgttacca ataagaagtt agttggtaaa agcgcttcgg aaattttaaa agaagcaggg 1020 atagcatgtg attttacccc tcgattaatt attgttgaaa cagatagatc ccatccattt 1080 gcaacggtag aacttttaat gccgattgtt ccagtggtac gagttgctga ttttgatcaa 1140 gcacttgaag tagcacttga gttagaacaa ggcttacatc acacggcaac aatgcattca 1200 caaaatatct ctagactgaa caaagcagca cgagatatgc aaacatccat tttcgtgaaa 1260 aatggaccat cgtttgctgg acttggcttt ggaggagaag gtagtgcaac tttcactatc 1320 gctaccccaa caggtgaagg aactactact gcgcgacact ttgctagacg ccgtcgttgt 1380 gttttaacag atggtttttc gattcgttaa 1410 <210> 32 <211> 1395 <212> DNA <213> Shewanella putrefaciens CN-32 <400> 32 ttagcgaatg gaaaatccat tggtcagcac acaacggcgt ttacgggcga agctccgtgc 60 tgatgttgtc ccttcgccag tgggtgtcgc aatagtaaag gtggtaaaac cctcggcacc 120 tatgcccagt ccggcatagg aagggccatt tttcacaaat attgaagtct gcatggtctt 180 cgcagccagg ttcagacggg taacattctg agaatgcatt atggcggtat ggtgctgctc 240 gttttccacc ttcagagcca gtgccagtcc tgtctcgaaa tcactgaccc gtacaacagg 300 cagaactggc attaattgtt cgaccatcac cagcggatcg tcctgctcca cttctacaat 360 aatcagtcgc ggtgctgttg atgtgttcag atcagcagcc tgcaggatca ctgccggact 420 tttacctacc agtttcttgt ttgcttctcc tttgtcattg ataacgacct tgcgcagtct 480 ggcaatatca ccgggcgttt ttaccagaaa ggcatcgttt ttctgcatat tatccatgag 540 gcgatcggcg atgctatcta ctacgatgac gcatttttcg gcgatacaaa ggacgttatg 600 atcgaaagag gcaccatcaa cgatatcttt ggcagccttg accggacagg cagtctcatc 660 caccagtaca ggggggttac cagggccagc accgataact tttttaccgg ttttcatggc 720 catattgaca attgccggac cacctgtaac cgcaagcaga gcaatgcgcg gatctgacat 780 catttcgcga gtagcgtcaa aggtgggctc tgctacggtg gtaaccagat tgcggatccc 840 gcttacccga taaatgatgt cttcgatttt ttcaataagc cacaaggata ccttttttgc 900 gcccggatgg gggctgaagt agacagcatt accagcggcc agcatactga tggtgttgtt 960 aatgatagtt tcggtcggat tggtgctggg ggcgatagcg ccaatgactc cgaagggaga 1020 tagttcgaat aaaaccatgc caccatcacc agtcagggcg cttgtggtca aatcctcaat 1080 ccccggagta ttattcagtg ccgcagtgtt tttactgatt ttgtcgggtg cattacccat 1140 gccggtttct tctgcggcgc gttcggacat ctctttgatc cagggtgcca gctcttcctt 1200 cagggctgta atgatcctgg ttcgaagtgc cagaggttct gccatgtatt ttttataggc 1260 atcgtagctg gcagtgatag catcttccac acgagcgaag atagtgtgct gaatgtttcc 1320 aggggcagta gcggtccctt tcagattatc agcaagaata ttgcggatca tattctccag 1380 ttcagtggta ttcat 1395 <210> 33 <211> 1395 <212> DNA <213> Kosakonia radicincitans DSM 16656 <400> 33 ttagcggatg gaaaatccat tagtcagtac acaacggcgt ttacgggcga agctccgtgc 60 agatgttgtc ccctcgccag tgggagttgc aatagtaaag gtggtaaaac cctcagcctc 120 aatgcccagc ccagcataag aagggccatt tttcacaaat attgaagttt gcatggtctt 180 cgcggccagg ttcagacggg aaacattctg cgaatgcatt atggctgtat ggtgctgatc 240 gttttccact ttcagcgcca gtgccagccc tgtctcaaaa tccctgaccc gcacaacagg 300 cagaaccggc atcaattgtt cgaccatcac cagcggatcg tcctgctcca cttccacaat 360 aatcagtcgc ggtgccgttg atgtgttcag atcagcagcc tgcaggatca ctgccgggct 420 tttgcctacc agtttcttgt ttgcttctcc tttgtcattg ataacgacct ggcgcagtct 480 ggcaatatca ccgggtgttt ttaccagaaa ggcatcgttt ttctgcatat tttctacgag 540 gcgatcggcg atgctatcga ccacgatgac gcatttttcg gcgatgcaaa ggacgttatg 600 atcgaaagag gcaccatcaa cgatatcttt ggcagctttg accggacagg cagtttcatc 660 caccagtaca ggggggttac ctgggccggc accgataact tttttaccgg ttttcatggc 720 catattgaca attgccgggc cgcctgtaac cacaagcaga gcaatgcgcg gatctgacat 780 catttcgcga gtggcgtcaa aggtgggctc tgcgacggtg gtaaccagat tccggatccc 840 gcttacccga taaatgatgt cttcgatttt ttcaataagc cacaaagata ccttctttgc 900 gccaggatgg gggctgaagt agacagcatt accagcggcc agcatactga tggtgttgtt 960 aatgatagtt tcggtcggat tggtgctggg ggcgatagcg ccaatgactc caaaggggga 1020 aagttcgaac aaaaccatgc caccatcgcc agtcagggcg cttgtggtca aatcctcaat 1080 ccctggagta ttattcagtg ccgcagtatt tttactgatt ttgtcgagtg cattacccat 1140 gccggtttct tctgcggcgc gttcggacat ctctttgatc cagggtgcca gctcttcctt 1200 cagggcagta ataatcctgg ttcgaagtgc cagaggttct gccaagtatt ttttataggc 1260 atcgtagctg gcagtgatag catcttccac acgagcgaag atagtgtgct gaatatttcc 1320 aggggcagta gcgatccctg tcagattatc agcaagaata gtgcggatca tattctccag 1380 ttcagtggta ttcat 1395 <210> 34 <211> 1404 <212> DNA <213> Tolumonas auensis DSM 9187 <400> 34 atgaataaca ctgagttaga aagcttaatc cgcactattc tgactgaaca gctcacgcct 60 tccgctacgg acacgcctgc atgtaccgct tcgtctgttg cactgtttga tgatgtggac 120 agtgccatct gtgcagcgca tgccgccttc ctgcgttatc aggaagcacc gttaaaaacc 180 cgcagtgcca ttattgccgc cattcgtgct gagattgcgc cctgcctgtc tgaactggca 240 gaacgtgctg ccgcagaaac cggtatgggc aacaccgccg acaagatcct gaaaaacaaa 300 gcggcactgg aaaatactcc cggtatcgaa gatttgaaaa caactgctct gaccggtgat 360 gaaggtatgg tgttgtttga atactctccg tttggggtag ttggtgccgt ggcgccaagc 420 acaaatccga ccgaaaccat tatcaataac agcatcagta tgctggccgc cggaaatgcg 480 atctatttca gcccgcatcc cggtgcaaaa aatatctctt tgtggttaat ccagaaaatg 540 gaagagatcg ccttcaaagt ctgcggtatc cacaatctga tcgtgacggt caaagagccg 600 acttttgaag ccacccagca aatgatggca catgacaaaa tcgcgttgtt agccatcacc 660 ggtggccccg gtatcgtgaa tatggggctg aaaagcggga aaaaagtgat tggtgccggc 720 gccggtaatc cgccttgtct ggtggatgaa accgcagaga tcgtcaaagc cgcacaagac 780 atcgtcgcgg gagcctcttt tgactacaac ctgccctgca tcgcagaaaa aagcgtgatt 840 gccgttgatt gcatcgccga tcaactgatt cagcaaatgc gcgaattcgg cgccatgcag 900 atcacggatc ctcaacaaat cgcgcagtta cgcgaagtct gcattcagaa aggtgcggct 960 aataagagcc tggtcggcaa aagcccggca acgattctgg cagccgcagg tattccctgc 1020 ccggccaaag aaccgcgact gatcattctg gaagtcccgg ccaatgaccc gtttgttgtt 1080 accgaacaac tgatgccggt gctgccgatt gttcgcgttg ataactttga acaaggcctg 1140 cagctggcac tgaaagtgga agatggcctg caccatacgg ccatgatgca ttcacagaat 1200 gtttcccgcc tgaacaaggc tgcacatctg atgcaaactt caattttcgt gaaaaacggc 1260 ccttcctacg caggaattgg tgtgggagca gaaggattca ccaccttcac cattgccacc 1320 ccgaccggcg aaggcaccac atcagcccgc acgttcggtc gcttacgccg ctgtgtactg 1380 accaatggct tttcaattcg ctaa 1404 <210> 35 <211> 1386 <212> DNA <213> Citrobacter koseri ATCC BAA-895 <400> 35 ttagcgaatt gaaaagccgt tggtcagtac gcagcgacgg gaacgggcaa atgtgcgtgc 60 tgaggttgtc ccttcgccgg tcggggtggc gatggtaaag gtggtaaacc cttcgccgcc 120 aacgccgata ccggcatagg aagggccgtt tttcacaaaa atagaggtct gtaaggtgcg 180 cgccgccaga ttcaggcgag agacattctg cgagtgcata atggcggtat ggtgcaggcc 240 ttcttcaact ttcagcgcca gtgccagcgc gctgtcgaaa ttatcgacct tcacgacggg 300 tagcattggc atcaactgtt cgctggttac ccacggatcg tcggcactga cgataccgat 360 aagcaaacgc ggcggttttg cgggaacggc aataccggcg gcttccagca tggcggcagg 420 gcttttcccg accagttttt tattcgcgtg accttccggg aggcagacgg cgcgtaattt 480 atcgatatcc gcagcgttta acagcaatgc gccgaaagcc tgcatttgct gaaccaggcg 540 ctcagcgacg ctctctacga caatcagact tttttctgcg atacagggca ggttgtaatc 600 gaacgctgcg ccgttgatga tatcttcggc cgctttgacg atatcggcgg tctcatcaac 660 gatgcagggc ggattgcccg ccccggcgcc aatgaccttt ttaccgcttt tcattcccat 720 cgcaacaatg ccggggccgc cagtaatagc cagcaccgca atattgggat gcgccatcat 780 ttgctgagtt gcctcaaagg tcggttctgc gacagtgacg accagattac ggatgccgca 840 gcagcggaag gcgatatctt cgatcatgcc gatcaatttg agtgagacgt tcttcgcgcc 900 aggatgcggg ctgaaataaa cgctgttgcc cgcagccagc atactgatgc tgttgttaat 960 aatggtttcg gtagggttgg tgctgggcgc gacggaacca atgacgccga acggtgaata 1020 ttcaaacagc accatgccgc catcgccggt gagggccgtg gttgtcaaat cctcaatgcc 1080 tggcgtgtta tccagcgcgg ctttgttttt aagaaattta tcttctttgt ttcccatccc 1140 cgtttccgct gcgctctccg ccgccagcgt ggcaagatgc ggcgtaagct cctggcgcag 1200 ggcgctgata atggcgctgc gcgttttgag cggacactgc tgataacgta agaaagcctg 1260 gtgcgccgcg tctatcgctt cgccgacgga ctgaaaaata ccgtgtcctt gcgtttcggc 1320 ctgcgcaggc gccaactgtt cgcttaaaat attacggatg agggtttcca gttcagaagt 1380 attcat 1386 <210> 36 <211> 1389 <212> DNA <213> Yersinia enterocolitica subsp. enterocolitica 8081 <400> 36 atgaatacca atgaccttga atcgctcatt cgcactatcc tcaccgagca actgacgccg 60 gtcacggccc ctgcctccag cgccattttt gccagcgtgg atgaagccat taatgctgct 120 cacagcgcgt ttttgcgcta tcagcaaagc ccgatgaaaa ctcgcagcgc cattatccgc 180 gctatccgtg agcaattaaa gccacaactt gtctctctgt ccgagcgcgg tgccagtgaa 240 accggcatgg gtaataaaga agataaattc ctgaaaaaca aagctgcact ggaaaacaca 300 ccgggtattg aagacttatc taccaccgcc ctgaccggtg atggcggcat ggtgttattc 360 gagtattcac ccttcggcgt tattggttca gtcaccccca gcactaaccc gaccgaaacc 420 attattaata acagcatcag tatgttggca gcgggtaatg cagtctattt cagcccccac 480 cctggtgcta aagccgtgtc actggatctc atcgcccaaa ttgaagagat cattttcaac 540 agttgcggca ttcgcaatct ggtggtgaca gtaaaagaac cgagtttcga agccacccaa 600 cagatgatgg cacacgacaa aattgcctta ctcgcgatta ctggtggccc ggccattgtg 660 gcgatgagca tgaaaagcgg caagaaagtg attggtgccg gtgcgggtaa cccaccttgt 720 ctggtggatg aaaccgccga gttagtcaaa gcggcgcagg atatcgtggc gggagcttca 780 tttgactaca acctgccgtg cattgcagag aaaagcctga tcgtggtgga aagtgttgcc 840 gaccgtttat tgcaacagat gcaggccttc gatgcattac tgataagcaa tccgcaagag 900 atcgacagct tacgcaaagc ctgcctgacg ccgcagggcc atgccaataa aaatctggtg 960 ggtaaaagtc caattgaact gctgaaagca gccggcatca cctgcccagc taaagccccg 1020 cgcctgttat tggtcgaagt agctggtgac gatccactgg tcaccaccga acaattgatg 1080 ccgctgttac cggtggtgcg ggtaaaggat tttgatgcgg ccctgacatt ggcactgcac 1140 gtcgagggcg gcctgcatca taccgcaacc atgcactcac aaaatgtctc gcgcttgaat 1200 ctggctgcac gtttgttgca aacctccatt tttgtcaaaa atggcccgtc ctatgctggg 1260 ataggggtcg gcggtgaagg ctttaccacc tttactattg ccaccccaac cggggagggt 1320 accacttcgg cgcgtacctt tgcgcgtcaa cgccgctgtg tactgactaa tggtttctct 1380 attcgctga 1389 <210> 37 <211> 1395 <212> DNA <213> Salmonella enterica subsp. enterica serovar Mbandaka str. ATCC 51958 <400> 37 ttagcgaata gaaaagccgt tggtcagtac gcagcgccgg gagcgggcaa aagtacgcgc 60 tgacgtggtc ccttcaccgg ttggcgtggc aatagtaaag gtggtaaagc cttcgccgcc 120 gacgccgatc ccggcataag aggggccgtt tttgacgaat atcgaggttt gcagggtgcg 180 ggctgcgagg ttcaggcgcg acacgttctg cgagtgcata atggcggtat gatgcagccc 240 ctcttcaacc ttcagggcca gcgccagcgc gctatcgaaa tcgctgactt ttaccaccgg 300 cagcatcggc atcagctgtt cgctggtgac ccacggatcg tcagcgctaa ccagcgcaat 360 cagcagacgc ggcgcttttg cagggacagc gatcccggcg gcttccagca tggccgatgg 420 gctcttgccg accagttttt tattcgcctg gccttcaggc aggcagacgg cgcggagttt 480 gtcggtatcg gcagggctta gcagcagcgc gccgaaggtt tgcatttgct gcaccagacg 540 ctcggcgacg ctctccacta cgatcaggct cttctcggca atgcagggca ggttataatc 600 gaatgacgcg ccgttgatga tatcttctgc cgctttcacc aggtccgctg tttcatcgac 660 gatgcagggc gggttacccg cgccagcgcc aatcaccttc ttaccgctct tcatgcccat 720 tgccacaatg cccgggccac cggtaatagc cagtaccgcg attcgcgggt gggccatcat 780 ctgctgggtc gcttcgaagg tgggttcagc tacagtcacc accagattgc ggatgccgca 840 gcagcggaag gcaatctctt caatcaggct aatcagcttc agagagacct ttttcgctcc 900 cggatgcggg ctaaagtaga cgctgttgcc cgccgccagc atgctgatgc tgttgttgat 960 gatggtttcc gtcgggttgg tgcttggggc gaccgaaccg atgacgccaa acggcgagta 1020 ttcaaagagc accatgccgc cgtcgccggt cagcgcggtg gtggtgagat cttctacgcc 1080 cggcgtgttg tccagcgcag ccttgttttt gaggagttta tcttctttgt tgcccatccc 1140 tgtttcattg gcactctctt ccgccagggt cgccagcagc ggcgtcagct cctgacgcat 1200 cgcgctgata atggcgctgc gggtttttag cgggcactgc tgataacgta agaacgcctg 1260 gtgcgcggca tcgatggcct cgctcacgga ctggaaaatc cctttgccct gaggctgggc 1320 cgtagtttgc gccggcgttg ttaattgctc gctaagaatg gtgcgaatca gggtttcgag 1380 ttcagaagta ttcat 1395 <210> 38 <211> 1389 <212> DNA <213> Yersinia mollaretii ATCC 43969 <400> 38 atgaacaccc atgatattga atctctcatt cgcactatcc tcaccgagca actgacgcct 60 gcgacggcct ctgccgtcag cgccattttt gccagcgtgg atgaagccgt gactgccgcc 120 cacagcgcct ttttgcgcta tcagcaaagc ccgatgaaaa cccgtagcgc cattatcagc 180 gccctgcgtg agcagttagc ccctcagttg gcgtcactct ctgagcgtgg tgccagcgaa 240 accggtatgg gcaacaaaga agataaattc ctgaaaaaca gggccgcgct ggagaatacc 300 cccggcatcg aagacctctc caccacggct ctgacgggcg acggcggtat ggtgctgttc 360 gaatattcgc cgttcggcgt gattggctct gtcgccccca gcactaaccc caccgaaacc 420 attatcaata acagcatcag catgttagcc gcgggtaatg cggtctattt tagcccgcac 480 cccggcgcta aagccgtctc actggatctg attgcccaaa ttgaagcgat cattttcaac 540 cgttgcggca tccgcaattt ggtggtgacg gtgcaagaac cgagctttga ggccacccaa 600 cagatgatgg cccacgacaa aatcgctcta ctggcgatca ccggtgggcc agccattgtg 660 gcgatgggca tgaagagcgg caaaaaagtg attggtgcgg gcgcgggtaa tccgccttgt 720 ctggtggatg agactgccga actggtgaaa gcggcgcaag atatcgtgtc cggcgcgtca 780 ttcgactaca acctgccctg cattgccgag aagagtttga ttgtggtgga gagtgtcgcc 840 gaccgcctgt tgcagcagat gcaagctttc gacgcgctgc tgatcactca gccgcaagag 900 gtcgatagcc tacgcaaagc ctgcctgacc ccccaaggcc acgctaacaa aaatctggtg 960 ggcaaaagcc cggctgaact gctgaaagcg gcgggtatca cttgccctgc caaagcccca 1020 cgcctactgc tggtggaagt ggcgggtgac gatccgctag tgaccacgga acaactgatg 1080 ccgctgctgc cagtggtgcg ggtaaaggat tttgatgcgg cgctgacact ggcgctgcaa 1140 gtggaaggcg gcctgcatca caccgcaacc atgcactccc agaatgtctc gcgcctgaat 1200 ctggcggccc gcctattgca gacctccatt tttgtcaaaa atggcccctc ctatgcgggg 1260 atcggggtcg gcggcgaggg ctttaccacc ttcaccatcg ccacccccac cggagagggc 1320 accacctcgg cccgcacctt tgcgcgtcaa cgccgctgtg tgctgactaa cggtttctcc 1380 attcgctga 1389 <210> 39 <211> 1383 <212> DNA <213> Escherichia fergusonii ATCC 35469 <400> 39 atgaataccc gcgaactgga aaacatcatc cgcaatattc tgcgcgaaca actgagcaca 60 acagcagatg ccccgacgaa tggcattttt gattctgttg atgaagcgat taatgccgcc 120 catcaggcct ttttgcgcta tcaacaatgc ccactgaaaa cccgtagcgc cattatcagc 180 gccattcgcc aggagctgac tccacatctc gatatgttgg cgacagaaag cgccaacgaa 240 acaggcatgg gcaataaaga ggataaattc ctcaaaaaca aagccgcgct cgataacaca 300 ccaggtattg aagacctgac cacaaccgcg ctcactggtg atggcggcat ggtgttattt 360 gaatattcgc cttttggtgt tattggttct gtgacgccga gcactaaccc aaccgaaacc 420 attattaaca acagtattag catgttagcc gctggaaaca gtgtctattt cagcccacat 480 ccgggggcaa aaaatatctc tttgaaattg attgccatga ttgaagagat cgcttttcgc 540 tgtagcggta tccacaacct gattgtcacc gttgctgaac caacatttga agccacacag 600 caaatgatga ctcaccccaa tatcgccgtt ctggcgatta ccggtggacc tggcattgtc 660 gcaatgggca tgaaaagcgg taaaaaagtc attggggctg gcgccggaaa tccgccatgc 720 atcgtagatg aaaccgcaga tctggtaaaa gctgcggaag atattattaa tggtgcctcg 780 tttgactaca acctgccctg cattgctgag aaaagcctga ttgtcgttga ggagattgca 840 ggtacgttgg tgcaacaaat gcagaatttt ggcgctctgc ttctcaacaa agaggaaacc 900 gataagttac gtgacgtttg tctgccacaa ggaatggcaa ataaacaact ggtaggtaaa 960 agtccggcag ctctgttgca ggcggcaggc attgctgtgc cgctaaaaac accacgtctg 1020 ttaattgccc ttgttgacgc ctgcgacaag tgggtaacca gcgaacaact tatgccaatg 1080 ctgccaatcg taaaagttaa ggatttcgat agcgcactga cgctggcact gaaagtggaa 1140 gaaggtttgc atcacaccgc cattatgcac tcgcaaaatg tttcgcgact caacctggca 1200 gcccggacct tacagacctc aatctttgtt aagaatggtc cgtcatatgc tggtatcggt 1260 gtcggtggtg aaggatttac cacctttacg atcgctaccc ccacgggtga aggtactacc 1320 tcggccaaaa cgtttgcccg ttcccgtcgt tgcgtgttga ccagcggttt ttcgatccgt 1380 taa 1383 <210> 40 <211> 1395 <212> DNA <213> Salmonella enterica subsp. enterica serovar Urbana str. ATCC 9261 <400> 40 ttagcgaata gaaaagccgt tggtcagcac gcagcgccgg gagcgggcaa aagtacgcgc 60 tgacgtggtc ccttcaccgg ttggcgtggc gatagtgaag gtggtaaagc cttcgccgcc 120 gacgccgatc ccggcataag aggggccgtt tttgacgaat atcgaggttt gcagcgtgcg 180 ggccgcgagg ttcaggcgcg acacgttctg cgagtgcata atggcggtat gatgcagccc 240 ctcttcaacc ttcagggcca gcgccagcgc gctatcgaaa tcgctgactt ttaccaccgg 300 cagcatcggc atcagctgtt cgctggtgac ccacggatcg tcagcgctaa ccagcgcaat 360 cagcagacgc ggcgcttttg cagggacagc gatcccggcg gcttccagca tggccgatgg 420 gctcttgccg accagttttt tattggcctg accttcaggc aggcagacgg cgcggagttt 480 gtcggtatcg gccgggctta gcagcagcgc gccgaaggtt tgcatttgct gcaccagacg 540 ctcggcgacg ctctccacta cgatcaggct cttctcggca atgcagggca ggttgtaatc 600 gaatgacgcg ccgttgatga tatcttccgc cgctttcacc aggtctgctg tttcatcgac 660 gatgcagggc gggttacccg cgccagcgcc aatcaccttc ttaccgctct tcatgcccat 720 tgccacaatg cccgggccac cggtaatggc cagtaccgcg attcgcgggt gggccatcat 780 ctgctgggtc gcttcgaagg tgggttcagc cacggtcacc accagattgc ggatgccgca 840 gcagcggaag gcaatctctt caattaggct aatcagcttc agagagacct ttttcgcgcc 900 cggatgcggg ctaaagtaaa tactattgcc cgccgccaac atgctgatgc tgttattgat 960 gatggtttcc gtcgggttgg tgcttggggc gaccgaaccg atgacgccaa acggtgagta 1020 ttcaaacagc accatgccgc cgtcgccggt cagcgcggtg gtggtgagat cttctacgcc 1080 cggcgtgttg tccagcgcag ccttgttttt gaggagttta tcttctttgt tgcccatccc 1140 tgtttcattg gcactctctt ccgccagggt cgccagcagc ggcgtcagct cctgacgcat 1200 cgcgctgata atggcgctgc gggtttttag cgggcactgc tgataacgta agaacgcctg 1260 gtgcgcggca tcgatggcct cgctcacgga ctggaaaatc cctttgccct taggctgggc 1320 cggcgtttgc gctggcgtgg ttaattgctc gctaagaatg gtgcgaatca gggtttcgag 1380 ttcagaagta ttcat 1395 <210> 41 <211> 299 <212> PRT <213> Dictyostelium discoideum (Slime mold) <400> 41 Met Ile Asn Arg Leu Phe Ser Ile Asn Asn Ile Lys Asn Gly Ser Lys 1 5 10 15 Phe Phe Ser Ser Ser Thr Thr Val Glu Thr Lys Gln Pro Leu Val Leu 20 25 30 Leu Glu Lys His Leu Val Asn Gly Lys Tyr Thr Gly Ile Gln Ile Val 35 40 45 Lys Leu Asn Lys Pro Lys Gln Leu Asn Ala Leu Thr Phe Glu Met Gly 50 55 60 Val Asp Tyr Lys Lys Val Val Asp Thr Leu Ala Glu Asp Lys Asp Leu 65 70 75 80 Lys Cys Val Val Leu Thr Gly Glu Gly Lys Ala Phe Ser Ala Gly Gly 85 90 95 Asp Leu Asp Phe Leu Ile Glu Arg Thr Lys Asp Thr Pro Glu Asn Asn 100 105 110 Gln Arg Ile Met Glu Arg Phe Tyr Arg Thr Phe Leu Tyr Ile Arg Ser 115 120 125 Leu Pro Val Pro Ile Ile Ser Ala Ile Asn Gly Ala Ala Ile Gly Ala 130 135 140 Gly Phe Cys Leu Ala Leu Ala Thr Asp Ile Arg Val Val Ser Asn Lys 145 150 155 160 Ala Pro Val Gly Leu Thr Phe Thr Lys Leu Gly Ile His Pro Gly Met 165 170 175 Gly Val Thr His Ser Ile Thr Asn Ile Val Gly Gln Asp Val Ala Ser 180 185 190 Tyr Met Leu Leu Ser Ser Asp Ile Ile Lys Gly Asp Glu Ala Gln Arg 195 200 205 Leu Gly Leu Val Leu Lys Ser Val Glu Ser Asp Gln Val Leu Pro Thr 210 215 220 Ala Leu Asn Leu Ala Glu Thr Ile Ser Lys Asn Ser Thr Ile Ala Val 225 230 235 240 Asn Ser Thr Thr Lys Thr Leu Arg Asn Lys Tyr Asn Ser Asp Leu Asp 245 250 255 Lys Ser Leu Thr Arg Glu Ala Asp Ala Gln Ser Gln Cys Trp Ala Ser 260 265 270 Lys Asp Ile Val Glu Gly Ile Leu Ala Ile Arg Glu Ser Arg Asp Pro 275 280 285 Lys His Asn Tyr Leu Leu Phe Asp Asp Gln Lys 290 295 <210> 42 <211> 261 <212> PRT <213> Clostridium acetobutylicum <400> 42 Met Glu Leu Asn Asn Val Ile Leu Glu Lys Glu Gly Lys Val Ala Val 1 5 10 15 Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Asp Thr 20 25 30 Leu Lys Glu Met Asp Tyr Val Ile Gly Glu Ile Glu Asn Asp Ser Glu 35 40 45 Val Leu Ala Val Ile Leu Thr Gly Ala Gly Glu Lys Ser Phe Val Ala 50 55 60 Gly Ala Asp Ile Ser Glu Met Lys Glu Met Asn Thr Ile Glu Gly Arg 65 70 75 80 Lys Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Arg Leu Glu Leu Leu 85 90 95 Glu Lys Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly 100 105 110 Cys Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Ser Asn Ala 115 120 125 Arg Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly 130 135 140 Gly Thr Gln Arg Leu Ser Arg Leu Val Gly Met Gly Met Ala Lys Gln 145 150 155 160 Leu Ile Phe Thr Ala Gln Asn Ile Lys Ala Asp Glu Ala Leu Arg Ile 165 170 175 Gly Leu Val Asn Lys Val Val Glu Pro Ser Glu Leu Met Asn Thr Ala 180 185 190 Lys Glu Ile Ala Asn Lys Ile Val Ser Asn Ala Pro Val Ala Val Lys 195 200 205 Leu Ser Lys Gln Ala Ile Asn Arg Gly Met Gln Cys Asp Ile Asp Thr 210 215 220 Ala Leu Ala Phe Glu Ser Glu Ala Phe Gly Glu Cys Phe Ser Thr Glu 225 230 235 240 Asp Gln Lys Asp Ala Met Thr Ala Phe Ile Glu Lys Arg Lys Ile Glu 245 250 255 Gly Phe Lys Asn Arg 260 <210> 43 <211> 155 <212> PRT <213> Clostridium difficile <400> 43 Asn Ser Lys Lys Val Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly 1 5 10 15 Gly Cys Glu Leu Ala Met Ala Cys Asp Ile Arg Ile Ala Ser Ala Lys 20 25 30 Ala Lys Phe Gly Gln Pro Glu Val Thr Leu Gly Ile Thr Pro Gly Tyr 35 40 45 Gly Gly Thr Gln Arg Leu Thr Arg Leu Val Gly Met Ala Lys Ala Lys 50 55 60 Glu Leu Ile Phe Thr Gly Gln Val Ile Lys Ala Asp Glu Ala Glu Lys 65 70 75 80 Ile Gly Leu Val Asn Arg Val Val Glu Pro Asp Ile Leu Ile Glu Glu 85 90 95 Val Glu Lys Leu Ala Lys Ile Ile Ala Lys Asn Ala Gln Leu Ala Val 100 105 110 Arg Tyr Ser Lys Glu Ala Ile Gln Leu Gly Ala Gln Thr Asp Ile Asn 115 120 125 Thr Gly Ile Asp Ile Glu Ser Asn Leu Phe Gly Leu Cys Phe Ser Thr 130 135 140 Lys Asp Gln Lys Glu Gly Ile Val Ser Phe Arg 145 150 155 <210> 44 <211> 258 <212> PRT <213> Clostridium pasteurianum <400> 44 Met Gly Asn Ile Ile Phe Glu Glu Glu Asp Gly Ile Glu Lys Val Thr 1 5 10 15 Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Glu Thr Leu Lys 20 25 30 Glu Leu Gly Thr Val Ile Asn Asp Ile Ser Val Asn Asp Gly Ile Lys 35 40 45 Ala Val Ile Ile Thr Gly Ser Gly Ser Lys Ala Phe Val Ala Gly Ala 50 55 60 Asp Ile Ala Glu Met Ser Thr Leu Asn Ser Ile Glu Ala Thr Asn Phe 65 70 75 80 Ser Arg Leu Ala Gln Asn Val Phe Ser Gln Ile Glu Asn Leu Pro Lys 85 90 95 Leu Val Val Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly Cys Glu 100 105 110 Leu Ala Met Ala Cys Asp Val Arg Phe Ala Ser Lys Lys Ala Lys Phe 115 120 125 Gly Gln Pro Glu Val Asn Leu Gly Ile Leu Pro Ser Phe Gly Gly Thr 130 135 140 Gln Arg Leu Pro Lys Leu Val Gly Lys Gly Ile Ala Lys Glu Leu Ile 145 150 155 160 Phe Ser Thr Asp Met Ile Thr Ala Asp Glu Ala Tyr Arg Ile Gly Leu 165 170 175 Ala Asn Lys Val Tyr Glu Pro Glu Glu Leu Leu Val Lys Ser Gln Glu 180 185 190 Phe Ala Glu Lys Val Met Thr Lys Ser Pro Trp Gly Val Lys Leu Ala 195 200 205 Lys Ala Cys Ile Asn Asn Gly Leu Asp Val Asp Leu Glu Ala Gly Leu 210 215 220 Lys Tyr Glu Ala Asn Ser Phe Gly Leu Cys Phe Ser Thr Glu Asp Gln 225 230 235 240 Lys Glu Gly Met Lys Ala Phe Leu Glu Lys Arg Lys Ala Asp Phe Lys 245 250 255 Gly Leu <210> 45 <211> 262 <212> PRT <213> Clostridium pasteurianum <400> 45 Met Asp Phe Asn Asn Ile Ile Leu Glu Lys Glu Glu Lys Ile Ala Val 1 5 10 15 Val Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ser Glu Thr 20 25 30 Leu Thr Glu Leu Asp Ser Val Ile Asp Glu Ile Asp Lys Asp Asn Glu 35 40 45 Ile Leu Ala Val Val Leu Thr Gly Ala Gly Lys Ser Phe Val Ala Gly 50 55 60 Ala Asp Ile Ser Glu Met Lys Asp Met Asn Val Val Glu Gly Arg Lys 65 70 75 80 Phe Gly Ile Leu Gly Asn Lys Val Phe Arg Lys Leu Glu Asn Leu Glu 85 90 95 Lys Pro Val Ile Ala Ala Leu Asn Gly Phe Thr Leu Gly Gly Gly Cys 100 105 110 Glu Ile Ala Met Ser Cys Asp Ile Arg Ile Ala Ser Thr Lys Ala Lys 115 120 125 Phe Gly Gln Pro Glu Val Gln Leu Gly Ile Thr Pro Gly Phe Gly Gly 130 135 140 Thr Gln Arg Leu Ala Arg Leu Ile Gly Pro Gly Ala Ala Lys Glu Leu 145 150 155 160 Ile Tyr Thr Gly Lys Ile Ile Asn Ala Glu Glu Ala Tyr Arg Leu Gly 165 170 175 Leu Val Asn Arg Val Ile Glu Pro Glu Thr Leu Leu Asp Glu Ala Lys 180 185 190 Gln Leu Ala Asn Thr Ile Ala Ala Asn Ala Pro Ile Ala Val Lys Leu 195 200 205 Ala Lys Ser Ala Ile Asn Arg Gly Ile Gln Thr Asp Ile Asp Thr Gly 210 215 220 Val Ser Ile Glu Ser Glu Val Phe Gly Ala Cys Phe Ser Thr Glu Asp 225 230 235 240 Gln Lys Glu Gly Met Asn Thr Phe Leu Asn Asp Lys Lys Tyr Leu Thr 245 250 255 Gly Asn Phe Lys Asn Lys 260 <210> 46 <211> 260 <212> PRT <213> Megasphaera elsdenii <400> 46 Met Asp Tyr Gln Asn Ile Ile Phe Ala Val Glu Asp Gly Ile Ala Thr 1 5 10 15 Ile Thr Ile Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Gln Ala Thr 20 25 30 Val Ser Glu Leu Lys Asp Val Val Glu Lys Ile Ala Ala Asp Lys Ala 35 40 45 Ile Lys Val Val Ile Ile Thr Gly Ala Gly Ala Lys Ser Phe Val Ala 50 55 60 Gly Ala Asp Ile Lys Glu Met Ala Ser Lys Asn Ala Ala Glu Gly Arg 65 70 75 80 Glu Trp Gly Gln Phe Gly Gln Asn Val Phe Thr Glu Ile Glu Asn Leu 85 90 95 Pro Gln Pro Val Ile Ala Ala Ile Asn Gly Phe Ala Leu Gly Gly Gly 100 105 110 Cys Glu Leu Ser Cys Ala Cys Asp Ile Arg Tyr Ala Ala Glu Asn Ala 115 120 125 Lys Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly 130 135 140 Gly Thr Gln Arg Leu Thr Arg Val Val Gly Arg Gly His Ala Lys Glu 145 150 155 160 Leu Ile Tyr Thr Gly Gly Met Ile Asp Ala Glu Lys Ala Lys Ala Ile 165 170 175 Gly Leu Val Asn Glu Val Phe Pro Gln Glu Glu Leu Met Pro Ala Ala 180 185 190 Val Lys Leu Ala Lys Lys Ile Ala Lys Asn Ala Pro Ile Ala Val Gln 195 200 205 Leu Ser Lys Ala Ala Ile Asn Arg Gly Ile Asn Cys Asp Val Val Thr 210 215 220 Gly Ile Ala Tyr Glu Ala Glu Val Phe Gly Leu Cys Phe Ser Thr Ala 225 230 235 240 Asp Gln Lys Glu Gly Met Ala Ala Phe Cys Glu Lys Arg Lys Ala Thr 245 250 255 Phe Glu Gly Lys 260 <210> 47 <211> 259 <212> PRT <213> Metallosphaera sedula <400> 47 Met Glu Phe Glu Thr Ile Glu Thr Lys Lys Glu Gly Asn Leu Phe Trp 1 5 10 15 Ile Thr Leu Asn Arg Pro Asp Lys Leu Asn Ala Leu Asn Ala Lys Leu 20 25 30 Leu Glu Glu Leu Asp Arg Ala Val Ser Gln Ala Glu Ser Asp Pro Glu 35 40 45 Ile Arg Val Ile Ile Ile Thr Gly Lys Gly Lys Ala Phe Cys Ala Gly 50 55 60 Ala Asp Ile Thr Gln Phe Asn Gln Leu Thr Pro Ala Glu Ala Trp Lys 65 70 75 80 Phe Ser Lys Lys Gly Arg Glu Ile Met Asp Lys Ile Glu Ala Leu Ser 85 90 95 Lys Pro Thr Ile Ala Met Ile Asn Gly Tyr Ala Leu Gly Gly Gly Leu 100 105 110 Glu Leu Ala Leu Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln 115 120 125 Leu Gly Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Tyr Gly Gly 130 135 140 Thr Gln Arg Leu Thr Arg Val Ile Gly Lys Gly Arg Ala Leu Glu Met 145 150 155 160 Met Met Thr Gly Asp Arg Ile Pro Gly Lys Asp Ala Glu Lys Tyr Gly 165 170 175 Leu Val Asn Arg Val Val Pro Leu Ala Asn Leu Glu Gln Glu Thr Arg 180 185 190 Lys Leu Ala Glu Lys Ile Ala Lys Lys Ser Pro Ile Ser Leu Ala Leu 195 200 205 Ile Lys Glu Val Val Asn Arg Gly Leu Asp Ser Pro Leu Leu Ser Gly 210 215 220 Leu Ala Leu Glu Ser Val Gly Trp Gly Val Val Phe Ser Thr Glu Asp 225 230 235 240 Lys Lys Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Glu Pro Thr Phe 245 250 255 Lys Gly Lys <210> 48 <211> 259 <212> PRT <213> Clostridicum kluyvery <400> 48 Met Glu Phe Lys Asn Ile Ile Leu Glu Lys Asp Gly Asn Val Ala Ser 1 5 10 15 Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Ala Ala Thr 20 25 30 Leu Lys Glu Ile Asp Ala Ala Ile Asn Asp Ile Ala Glu Asp Asp Asn 35 40 45 Val Tyr Ala Val Ile Ile Thr Gly Ser Gly Lys Ala Phe Val Ala Gly 50 55 60 Ala Asp Ile Ala Glu Met Lys Asp Leu Thr Ala Val Glu Gly Arg Lys 65 70 75 80 Phe Ser Val Leu Gly Asn Lys Ile Phe Arg Lys Leu Glu Asn Leu Glu 85 90 95 Lys Pro Val Ile Ala Ala Ile Asn Gly Phe Ala Leu Gly Gly Gly Cys 100 105 110 Glu Leu Ser Leu Ser Cys Asp Ile Arg Ile Ala Ser Ser Lys Ala Lys 115 120 125 Phe Gly Gln Pro Glu Val Gly Leu Gly Ile Thr Pro Gly Phe Gly Gly 130 135 140 Thr Gln Arg Leu Ala Arg Ala Ile Gly Val Gly Met Ala Lys Glu Leu 145 150 155 160 Ile Tyr Thr Gly Lys Val Ile Asn Ala Glu Glu Ala Leu Arg Ile Gly 165 170 175 Leu Val Asn Lys Val Val Glu Pro Asp Lys Leu Leu Glu Glu Ala Lys 180 185 190 Ala Leu Val Asp Ala Ile Ile Val Asn Ala Pro Ile Ala Val Arg Met 195 200 205 Cys Lys Ala Ala Ile Asn Gln Gly Leu Gln Cys Asp Ile Asp Thr Gly 210 215 220 Val Ala Tyr Glu Ala Glu Val Phe Gly Glu Cys Phe Ala Thr Glu Asp 225 230 235 240 Arg Val Glu Gly Met Thr Ala Phe Val Glu Lys Arg Asp Lys Ala Phe 245 250 255 Lys Asn Lys <210> 49 <211> 502 <212> PRT <213> Sulfolobus tokodaii <400> 49 Met Ala Ile Arg Thr Gly Glu Gln Tyr Leu Asp Ser Ile Lys Ile Arg 1 5 10 15 Asn Lys Ala Glu Ile Tyr Val Met Gly Lys Glu Val Lys Asp Val Thr 20 25 30 Thr His Pro Phe Leu Lys Pro Ser Val Met Ala Phe Lys Ala Thr Phe 35 40 45 Asp Ala Ala Trp Glu Glu Asp Thr Lys Glu Leu Ala Arg Ala Trp Ser 50 55 60 Pro Phe Ile Asn Glu Glu Val Asn Arg Phe Asn His Ile His Arg Ser 65 70 75 80 Pro Glu Asp Leu Ala Ala Lys Val Lys Leu Leu Arg Lys Leu Ser His 85 90 95 Lys Thr Gly Ala Cys Phe Gln Arg Cys Val Gly Trp Asp Ala Leu Asn 100 105 110 Thr Leu Trp Ile Met Thr Asn Ile Met Ala Gln Lys Gly Lys Lys Glu 115 120 125 Tyr Lys Asp Arg Phe Val Glu Tyr Leu Ser Tyr Val Gln Lys Lys Asp 130 135 140 Leu Ala Leu Ala Gly Ala Met Thr Asp Ala Lys Gly Val Arg Thr Leu 145 150 155 160 Lys Pro His Gln Gln Pro Asn Lys Asn Ala Tyr Val Arg Ile Glu Glu 165 170 175 Val Thr Lys Asp Gly Ile Tyr Val Ser Gly Ala Lys Ala Asn Ile Thr 180 185 190 Gly Val Ala Ala Thr Glu Glu Ile Val Val Leu Pro Thr Arg Ala Met 195 200 205 Gly Pro Glu Asp Lys Asp Tyr Ala Val Ala Phe Ser Ile Pro Thr Asp 210 215 220 Thr Glu Gly Ile Lys Ile Ile Val Gly Arg Gln Leu Asn Asp Ala Arg 225 230 235 240 Arg Leu Glu Gly Gly Asp Ile Asp Ala Leu Pro Tyr Phe Tyr Asn His 245 250 255 Glu Gly Leu Val Ile Phe Asp His Val Phe Val Pro Met Asp Arg Val 260 265 270 Phe Leu Met Gly Glu Tyr Glu Phe Thr Ser Gln Leu Val Glu Val Phe 275 280 285 Ser Ala Tyr His Arg Gln Gly Tyr Gly Gly Cys Lys Ala Gly Leu Gly 290 295 300 Asp Val Ile Ile Gly Ala Ser Met Asn Leu Ala Lys Gln Leu Gly Val 305 310 315 320 Glu Lys Ala Ser His Val Gln Glu Lys Leu Thr Glu Met Ile Phe Leu 325 330 335 Thr Glu Thr Met Tyr Ser Ala Gly Ile Ala Ala Ser Leu Asn Ala Val 340 345 350 Lys Val Cys Asp Asn Cys Trp Trp Val Asn Pro Met His Ala Asn Val 355 360 365 Thr Lys His Leu Val Ala Arg Phe Pro Ala Gln Ile Ser Gln Leu Ser 370 375 380 Ile Asp Ile Ala Gly Gly Ile Ile Gly Thr Ala Pro Ser Glu Trp Asp 385 390 395 400 Leu Lys Asn Pro Lys Leu Arg Glu Tyr Ile Ala Lys Tyr Leu Gln Gly 405 410 415 Val Glu Gly Tyr Thr Ala Glu Asp Arg Leu Arg Met Val Arg Leu Leu 420 425 430 Glu Asn Val Ser Leu Gly Val Ala Phe Gln Ile Glu Ser Val His Gly 435 440 445 Ala Gly Ser Pro Ala Ala Gln Arg Ile Met Phe Ser Arg Leu Tyr Asp 450 455 460 Leu Asn Tyr Ala Glu Glu Val Ala Lys Arg Leu Ala Gly Lys Lys Thr 465 470 475 480 Asp Leu Gln Trp Lys Pro Lys Ala Glu Pro Trp Arg Glu Ser Glu Thr 485 490 495 Glu Lys Leu Val Lys Ser 500 <210> 50 <211> 483 <212> PRT <213> Geobacter metallireducens <400> 50 Met Ala Leu Arg Asp Gly Asn Ser Tyr Arg Glu Ser Leu Arg Ala Leu 1 5 10 15 Asn Ile Lys Val Tyr Ala Phe Gly Glu Lys Ile Asp Ser Ile Val Asp 20 25 30 His Pro Leu Phe Gln Pro His Ile Asn Ala Ala Ala Leu Thr Phe Asp 35 40 45 Leu Ala His Asp Pro Thr Thr Glu Ala Leu Val Thr Ala Thr Ser His 50 55 60 Leu Thr Gly Ser Lys Ile Ser Arg Phe Thr His Ile His Gln Ser Thr 65 70 75 80 Asp Asp Leu Ile Lys Lys Val Lys Met Leu Arg Leu Ile Ala Gly Lys 85 90 95 Thr Gly Ser Cys Tyr Gln Arg Cys Val Gly Trp Asp Ala Leu Asn Ala 100 105 110 Asn Tyr Thr Val Thr Tyr Glu Met Asp Gln Glu Leu Gly Thr Asp Tyr 115 120 125 His Gln Arg Phe Arg Arg Tyr Leu Glu Tyr Ile Gln Asp Asn Asp Leu 130 135 140 Met Val Ala Gly Ala Met Thr Asp Pro Lys Gly Asp Arg Gly Leu Pro 145 150 155 160 Pro Ala Lys Gln Lys Asp Pro Asp Met Phe Val His Val Val Ala Lys 165 170 175 Asn Asp Lys Gly Ile Val Ile Arg Gly Ala Lys Val His Gln Thr Gly 180 185 190 Ile Val Asn Ser His Glu Met Leu Ile Met Pro Thr Met Ala Met Gly 195 200 205 Glu Glu Asp Gly Asp Tyr Ala Val Ala Cys Ala Leu Pro Thr Asp Ser 210 215 220 Pro Gly Val Ile His Ile Phe Gly Arg Gln Thr Asn Asp Thr Arg Arg 225 230 235 240 Leu Glu Lys Gly Asp Leu Asp Gln Gly Asn Ala Glu Tyr Gly Thr Val 245 250 255 Gly Gly Glu Ala Leu Thr Ile Leu Glu Asp Val Phe Val Pro Trp Glu 260 265 270 Arg Val Phe Met Cys Gly Glu Tyr Lys Tyr Ala Gly Leu Leu Val Glu 275 280 285 Arg Phe Ala Ser Tyr His Arg Gln Asn Tyr Gly Gly Cys Lys Ala Gly 290 295 300 Val Ser Asp Val Ile Ile Gly Ala Thr Thr Ala Met Ala Glu Tyr Asn 305 310 315 320 Gly Ala Ala Lys Ala Ser His Val Arg Asp Lys Ile Val Glu Met Val 325 330 335 His Leu Thr Glu Thr Leu Tyr Cys Gly Ser Ile Ala Cys Ser Cys Glu 340 345 350 Gly Ala Pro Thr Pro Ser Gly Ala Tyr Phe Val Asn Pro Leu Leu Ala 355 360 365 Asn Thr Val Lys Gln Asn Val Thr Arg Phe Ile Tyr Glu Ile Ala Arg 370 375 380 Leu Ser His Asp Ile Ser Gly Gly Cys Met Ala Thr Met Pro Ser Glu 385 390 395 400 Lys Asp Leu His His Asp Glu Ile Gly Lys Tyr Val Glu Lys Tyr Phe 405 410 415 Arg Gly Val Asp Glu Ala Pro Thr Glu Glu Arg Met Arg Met Ala Arg 420 425 430 Leu Val Glu Asn Met Thr Gly Gly Thr Ala Leu Val Glu Ser Met His 435 440 445 Gly Ala Gly Ser Pro Gln Ala Gln Arg Val Met Ile Leu Arg Gln Ala 450 455 460 Asn Leu Gly His Lys Val Lys Leu Ala Lys Lys Leu Ala Gly Ile Lys 465 470 475 480 Glu Glu Lys <210> 51 <211> 463 <212> PRT <213> Sulfolobus solfataricus <400> 51 Met Arg Ser Lys Glu Asp Phe Leu Lys Ser Leu Lys Asp Gly Arg Asn 1 5 10 15 Leu Tyr Tyr Arg Gly Lys Leu Val Glu Asp Ile Thr Thr His Gln Ile 20 25 30 Leu Lys Thr Ala Ala Leu His Ala Ala Lys Leu Tyr Glu Tyr Ala Asp 35 40 45 Arg Val Tyr Glu Asp Asn Lys Met Gly Lys Met Ser Lys Phe Phe Lys 50 55 60 Val Pro Trp Thr Ser Gln Asp Leu Leu Asp Arg His Lys Leu Ile Tyr 65 70 75 80 Asp Leu Thr Met Tyr Cys Asn Gly Val Phe Asn Ile Ser Gln Ala Ile 85 90 95 Gly Ser Asp Ala Ile Phe Ala Leu Met Ile Thr Ala Lys Gln Val Asp 100 105 110 Arg Lys Tyr Gly Thr Asp Tyr Ser Lys Arg Val Glu Lys Tyr Phe Glu 115 120 125 Arg Val Ala Lys Glu Asp Leu Thr Leu Ala Thr Ala Gln Thr Asp Val 130 135 140 Lys Gly Asp Arg Ser Lys Arg Pro Ser Glu Gln Val Asp Pro Asp Met 145 150 155 160 Tyr Val Arg Val Val Asp Val Lys Ser Asp Gly Ile Val Val Arg Gly 165 170 175 Ala Lys Ala His Thr Thr Gln Ser Ala Val Ser Asp Glu Ile Ile Val 180 185 190 Ile Pro Thr Arg Val Met Arg Asp Ser Asp Lys Asp Tyr Ala Val Ala 195 200 205 Phe Ala Val Pro Ala Asn Thr Lys Gly Leu Lys Met Tyr Ile Arg Pro 210 215 220 Ile Asp Glu Ile Glu Gly Asn Ser Ser Ser Val Leu Ser Arg Lys Asp 225 230 235 240 Tyr Glu Leu Glu Thr Leu Thr Val Phe Asn Asp Val Phe Val Pro Trp 245 250 255 Asp Arg Val Phe Leu Phe Lys Glu Tyr Asp Tyr Ala Gly Thr Leu Ala 260 265 270 Met Leu Phe Ala Thr Phe His Arg Phe Thr Ala Leu Ser Tyr Arg Ser 275 280 285 Ala Thr Met Asn Leu Tyr Leu Gly Ala Ser Lys Val Ala Ser Gln Val 290 295 300 Asn Gly Ile Glu Asn Glu Lys His Val Arg Asp Asp Ile Val Asp Ile 305 310 315 320 Ile Leu Tyr Lys Glu Ile Met Arg Ser Ser Ala Ile Ala Ala Ala Val 325 330 335 Tyr Pro Val Asn Met Glu Gly Ile Ala Val Pro Asn Pro Leu Phe Thr 340 345 350 Asn Val Gly Lys Leu Tyr Ser Asn Met His Phe His Asp Val Val Arg 355 360 365 Asp Leu Ile Asp Ile Ala Gly Gly Ile Ile Ala Thr Met Pro Ser Gln 370 375 380 Glu Asp Leu Glu Ser Asp Glu Gly Lys Asn Ile Val Lys Tyr Leu Arg 385 390 395 400 Gly Ser Val Asp Gly Glu Glu Arg Ala Lys Val Leu Lys Leu Ala Lys 405 410 415 Glu Leu Gly Ala Ser Thr Phe Thr Gly Tyr Leu Leu Thr Gly Met Ile 420 425 430 His Ala Glu Gly Ser Met Glu Ala Ser Lys Ile Glu Leu Phe Arg Ser 435 440 445 Tyr Asn Phe Lys Glu Ala Glu Asn Leu Val Lys Arg Val Leu Ser 450 455 460 <210> 52 <211> 479 <212> PRT <213> Syntrophobacter fumaroxidans <400> 52 Met Gly Leu Lys Thr Lys Ala Glu Tyr Ile Glu Ser Leu Arg Gly Met 1 5 10 15 Lys Pro Thr Val Tyr Met Phe Gly Glu Lys Ile Glu Ser Val Val Asp 20 25 30 Asn Pro Arg Leu Arg Ala Gly Ile Glu Ala Thr Gly Ala Thr Tyr Glu 35 40 45 Leu Ala Glu Thr Glu Glu Tyr Arg Pro Leu Ile Val Thr Glu Ser Pro 50 55 60 Leu Ile His Glu Pro Val Asn Arg Tyr Thr Leu Pro Pro Ser Ser Ile 65 70 75 80 Ala Asp Leu Val Ala Arg Val Lys Ile Asn Arg Leu Met Gly Thr Arg 85 90 95 Val Gly Thr Cys Phe Gln Arg Cys Thr Gly Leu Asp Cys Leu Ser Ala 100 105 110 Leu Ser Ile Val Thr Tyr Asp Ile Asp Ala Lys His Ser Thr Pro Tyr 115 120 125 Phe Lys Arg Phe Ile Glu Phe Leu Lys His Val Gln Lys Asn Asp Leu 130 135 140 Thr Cys Asn Ala Gly Val Thr Asp Val Lys Gly Asp Arg Ser Leu Ala 145 150 155 160 Pro His Glu Gln Glu Asp Lys Asp Met Tyr Val Arg Val Val Glu Arg 165 170 175 Asn Ala Asp Gly Ile Val Val Arg Gly Ala Lys Ala His Gln Thr Gly 180 185 190 Ser Leu Ser Ser His Glu Ile Ile Val Leu Pro Thr Arg Ala Leu Arg 195 200 205 Lys Gly Asp Glu Asp Tyr Ala Leu Ala Phe Ala Ile Pro Asn Asp Thr 210 215 220 Pro Gly Leu Ile His Val Val Gly Arg Ser Ser Leu Asp Thr Arg Gln 225 230 235 240 Leu Asp Gly Cys Asp Leu Gly Asn Leu His Tyr Ser Lys Tyr Cys Pro 245 250 255 Thr Val Ile Phe Lys Asp Val Phe Val Pro Trp Glu Arg Val Phe Met 260 265 270 Cys Gly Glu Val Glu Phe Ala Val Glu Met Val Asn Arg Phe Ser Ala 275 280 285 Tyr His Arg Gln Ser His Gly Gly Cys Lys Ser Gly Lys Ile Asp Cys 290 295 300 Met Val Gly Ala Ala Leu Thr Met Met Asp Tyr Asn Gly Thr Glu Lys 305 310 315 320 Ala Gly His Leu Lys Gln Lys Ala Ile Glu Met Val His Arg Ala Glu 325 330 335 Thr Leu Tyr Gly Cys Ser Leu Ala Ala Ser Tyr Glu Gly Lys Lys Glu 340 345 350 Pro Ser Gly Thr Tyr Phe Ile Asp Thr Val Leu Ala Asn Ala Ser Lys 355 360 365 Ile His Glu Gly Lys Glu Met Ser Glu Ala Gly Arg Leu Leu Val Asp 370 375 380 Ile Ala Gly Gly Phe Val Ala Asp Leu Pro Ser Asp Arg Asp Leu Ala 385 390 395 400 Ile Pro Glu Val Gly Glu Leu Leu Lys Lys Tyr Leu Lys Gly Val Ala 405 410 415 Ser Val Pro Val Glu Asp Arg Val Lys Met Tyr Arg Leu Ile Glu Lys 420 425 430 Leu Val Met Glu Ser Ala Asp Thr Ile Ser Asp Ile His Gly Gly Gly 435 440 445 Ser Pro Glu Ala His Arg Ile Thr Ile Leu Arg Glu Ser Asn Leu Lys 450 455 460 Ala Lys Lys Asp Ala Ala Lys Arg Leu Ala Gly Ile Glu Ser Lys 465 470 475 <210> 53 <211> 486 <212> PRT <213> Porphyromonas gingivalis <400> 53 Met Met Thr Ser Glu Gln Tyr Val Glu Ser Leu Arg Lys Leu Asn Leu 1 5 10 15 Lys Val Tyr Phe Met Gly Glu Arg Ile Glu Asn Pro Val Asp His Pro 20 25 30 Met Ile Arg Pro Ser Met Asn Ser Val Ala Met Thr Tyr Lys Leu Ala 35 40 45 Glu Met Asp Glu Tyr Lys His Leu Met Thr Ala Thr Ser Asn Leu Thr 50 55 60 Gly Lys Gln Val Asn Arg Phe Cys His Leu His Gln Ser Thr Glu Asp 65 70 75 80 Leu Lys Asp Lys Val Lys Met Gln Arg Leu Met Gly Gln Lys Thr Ala 85 90 95 Ser Cys Phe Gln Arg Cys Val Gly Met Asp Ala Phe Asn Ala Ile Tyr 100 105 110 Ser Thr Thr Tyr Glu Met Asp Gln Ala Leu Gly Thr Thr Tyr His Lys 115 120 125 Arg Phe Ile Glu Tyr Met Lys Tyr Val Gln Asp Asn Asp Leu Val Val 130 135 140 Asp Gly Ala Met Thr Asp Pro Lys Gly Asp Arg Gly Leu Ser Pro Ser 145 150 155 160 Glu Gln Ala Asp Pro Asp Leu Tyr Leu His Ile Val Glu Val Arg Glu 165 170 175 Asp Gly Ile Val Val Ser Gly Ala Lys Ala His Gln Thr Gly Ala Val 180 185 190 Asn Ser His Glu His Leu Ile Met Pro Thr Ile Ala Met Arg Glu Ala 195 200 205 Asp Ala Asp Tyr Ala Val Ser Phe Ala Val Pro Ser Asp Ala Glu Gly 210 215 220 Val Ile Met Ile Tyr Gly Arg Gln Ser Cys Asp Thr Arg Lys Met Glu 225 230 235 240 Glu Gly Ala Asp Ile Asp Leu Gly Asn Ser Glu Phe Gly Gly His Glu 245 250 255 Ala Leu Val Val Phe Asp Arg Val Phe Val Pro Asn Asp Arg Val Phe 260 265 270 Met Cys Lys Glu Tyr Gln Phe Ala Gly Met Met Val Glu Arg Phe Ala 275 280 285 Gly Tyr His Arg Gln Ser Tyr Gly Gly Cys Lys Val Gly Val Gly Asp 290 295 300 Val Leu Ile Gly Ala Ala Ala Leu Ala Ala Asp Tyr Asn Gly Val Pro 305 310 315 320 Lys Ala Ser His Ile Lys Asp Lys Leu Ile Glu Met Ile His Leu Asn 325 330 335 Glu Thr Leu Tyr Ala Cys Gly Ile Ala Cys Ser Ser Glu Gly Thr Gln 340 345 350 Met Lys Ala Gly Asn Tyr Met Ile Asp Leu Leu Leu Ala Asn Val Cys 355 360 365 Lys Gln Asn Ile Thr Arg Leu Pro Tyr Glu Ile Ala Arg Leu Ala Glu 370 375 380 Asp Ile Ala Gly Gly Leu Met Val Thr Met Pro Ser Gln Gln Asp Phe 385 390 395 400 Arg His Pro Glu Ile Gly Pro Ile Val Lys Lys Tyr Leu Ala Gly Ala 405 410 415 Thr Gly Lys Ser Thr Glu Asn Arg Met Arg Val Leu Arg Leu Ile Glu 420 425 430 Asn Ile Thr Leu Gly Thr Ala Ala Val Gly Tyr Arg Thr Glu Ser Met 435 440 445 His Gly Ala Gly Ser Pro Gln Ala Gln Arg Ile Met Ile Ala Arg Gln 450 455 460 Gly Asp Leu Glu Gly Lys Lys Lys Leu Ala Arg Ala Ile Ala His Ile 465 470 475 480 Asp Glu Ser Leu Asp Lys 485 <210> 54 <211> 529 <212> PRT <213> Polynucleobacter necessarius subsp. Asymbioticus <400> 54 Met Ser Gln Ser Thr Ser Gln Phe Met Asn Ser Lys Asp Tyr Gln Glu 1 5 10 15 Ser Leu Arg Ser Leu Lys Pro Thr Val Tyr Val Asp Gly Arg Leu Ile 20 25 30 Glu Ser Val Ala Asp Glu Pro Ser Leu Arg Pro Gly Val Gln Ala Leu 35 40 45 Gly Val Thr Tyr Asp Met Val His Asp Pro Ala Leu Ala Pro Leu Met 50 55 60 Leu Ala Asp Ser Asn Gly Thr Pro Val Pro Arg Met Leu His Ile Asn 65 70 75 80 Gln Ser Ser Gly Asp Leu Leu Asn Lys Leu Glu Ala Val Arg Val Leu 85 90 95 Cys Gln Glu Thr Gly Cys Ala Gln Arg Tyr Leu Ala His Asp Ala Leu 100 105 110 Asn Ala Ile Ala Gln Val Ser Ala Arg Ile Asp Asp Ala Lys Gly Ser 115 120 125 Asn Glu His Ser Ala Lys Phe Ser Glu Tyr Leu Ser His Val Gln Thr 130 135 140 Lys Asp Leu Ala Leu Gly Ile Ala Met Thr Asp Ala Lys Gly Asp Arg 145 150 155 160 Ser Arg Arg Pro His Glu Gln Glu Asn Pro Asp Thr Tyr Val His Ile 165 170 175 Val Ser Gln Asp Ala Lys Gly Val Val Ile Ser Gly Thr Lys Ala Ile 180 185 190 Val Thr Gly Ala Pro Tyr Met His Glu Phe Leu Val Met Pro Gly Arg 195 200 205 Asn Met Thr Lys Glu Asp Ala Ala Phe Ala Ile Cys Cys Ala Val Pro 210 215 220 Val Asp Ala Lys Gly Ile Thr Ile Val Ala Arg Pro Ala Gly Arg Pro 225 230 235 240 Gly Asp Lys Val Glu His Gly Lys Pro Ile Phe Ser Ser Lys Tyr Gly 245 250 255 Gln Ser Thr Gly Val Val Ile Phe Asp Lys Val Phe Val Pro Trp Asp 260 265 270 Arg Val Phe Tyr Ala Gly Glu Trp Glu His Ser Ser Val Leu Thr Tyr 275 280 285 Asn Tyr Ala Thr His His Arg His Ser Cys Ile Ala Ala Arg Ala Gly 290 295 300 Phe Gly Asp Leu Leu Ile Gly Ala Gly Ala Leu Met Cys Glu Ala Asn 305 310 315 320 Gly Leu Asp Pro Ala Thr Lys Ser Asn Leu Arg Asp Pro Met Val Glu 325 330 335 Leu Ile Lys Ile Thr Glu Gly Phe Tyr Ala Cys Gly Val Ala Ala Ser 340 345 350 Val Tyr Gly Thr Gln Asp Pro Tyr Ser Lys Ser Phe Met Pro Glu Pro 355 360 365 Val Phe Ser Asn Ile Gly Lys Leu Leu Leu Ala Thr Gln Ile Tyr Asp 370 375 380 Met His Arg Leu Ala His Glu Val Ser Gly Gly Leu Ile Val Ala Leu 385 390 395 400 Pro Gly Pro Asp Glu Asp His Asn Pro Ala Thr Ala Ala Thr Leu Ala 405 410 415 Glu Val Leu Arg Ala Asn Pro Ala Val Pro Tyr Asp Lys Arg Ile Glu 420 425 430 Val Ala Arg Phe Ile Glu Asp Leu Thr Ala Ser Tyr Gln Gly Gly Trp 435 440 445 Tyr Ser Val Ile Ser Leu His Gly Gly Gly Ser Pro Ala Ala Met Lys 450 455 460 Gln Glu Ile Tyr Arg Gln Tyr Pro Ile Gly Asn Lys Val Glu Leu Val 465 470 475 480 Glu Arg Leu Leu Asp Arg Gly Val Leu Thr Ser Ser Glu Glu Arg Ala 485 490 495 Ile Thr Lys Asn Lys Gln Pro Gly Arg Cys Cys Asp Gln Gly Cys Ser 500 505 510 Ala Pro Gly Gln Ala Val Met Val Pro Leu Pro Glu Pro Gly Arg Arg 515 520 525 Thr <210> 55 <211> 257 <212> PRT <213> Sulfolobus tokodaii <400> 55 Met Glu Thr Ile Val Ile Lys Lys Glu Thr Pro Ile Gly Trp Ile Tyr 1 5 10 15 Leu Asn Arg Pro Asp Arg Leu Asn Ala Ile Asn Gln Gln Met Ile Lys 20 25 30 Glu Leu Arg Gln Gly Ile Asp Glu Met Val Tyr Asp Ser Asp Ile Lys 35 40 45 Val Ile Ile Ile Thr Gly Asn Gly Lys Ala Phe Ser Ala Gly Ala Asp 50 55 60 Ile Ser Arg Phe Lys Glu Leu Asn Gly Tyr Thr Ala Trp Gln Phe Ala 65 70 75 80 Lys Ser Gly Arg Glu Leu Met Asp Tyr Ile Glu Asn Ile Ser Lys Pro 85 90 95 Thr Ile Ala Met Val Asn Gly Tyr Ala Leu Gly Gly Gly Leu Glu Leu 100 105 110 Ala Met Ala Cys Asp Ile Arg Ile Ala Ala Glu Glu Ala Gln Leu Gly 115 120 125 Leu Pro Glu Ile Asn Leu Gly Ile Tyr Pro Gly Phe Gly Gly Thr Gln 130 135 140 Arg Leu Val Arg Leu Ile Gly Lys Gly Lys Ala Leu Glu Leu Met Leu 145 150 155 160 Thr Gly Asp Arg Ile Ser Ala Lys Glu Ala Glu Lys Ile Gly Leu Val 165 170 175 Asn Lys Val Val Pro Leu Ser Asn Leu Glu Gln Glu Thr Arg Asn Phe 180 185 190 Ala Leu Lys Leu Ala Glu Lys Pro Pro Ile Ser Ile Ala Leu Ile Lys 195 200 205 Leu Leu Val Asn Gln Gly Ile Asp Leu Pro Ile Leu Ala Gly Leu Asn 210 215 220 Met Glu Ser Leu Gly Trp Gly Val Val Phe Ser Thr Glu Asp Glu Lys 225 230 235 240 Glu Gly Val Ser Ala Phe Leu Glu Lys Arg Lys Ala Gln Phe Lys Gly 245 250 255 Lys <210> 56 <211> 258 <212> PRT <213> Gordonia terrae C-6 <400> 56 Met Thr Glu His Gln Thr Ile Val Val Glu Thr Ser Gly Arg Val Gly 1 5 10 15 Ile Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ala Leu Asn Thr Glu 20 25 30 Leu Met Asn Glu Val Val Gly Ala Val Lys Glu Phe Asp Val Asp Gln 35 40 45 Gly Ile Gly Ala Ile Val Ile Thr Gly Ser Glu Lys Ala Phe Ala Ala 50 55 60 Gly Ala Asp Ile Lys Glu Met Ser Ser Lys Ser Tyr Ala Asp Val Val 65 70 75 80 Asn Glu Gln Phe Phe Gly Ala Trp Asp Glu Leu Ser Arg Ala Arg Thr 85 90 95 Pro Ile Ile Ala Ala Val Thr Gly Tyr Ala Leu Gly Gly Gly Cys Glu 100 105 110 Leu Ala Met Leu Cys Asp Thr Ile Ile Ala Gly Asp Asn Ala Val Phe 115 120 125 Gly Gln Pro Glu Ile Asn Leu Gly Val Ile Pro Gly Ile Gly Gly Ser 130 135 140 Gln Arg Leu Thr Arg Ala Val Gly Lys Ala Lys Ala Met Asp Met Val 145 150 155 160 Leu Thr Gly Arg Gln Met Lys Val Asp Glu Ala Glu Arg Leu Gly Leu 165 170 175 Val Ser Arg Val Val Pro Lys Glu Asp Cys Arg Ala Ala Ala Ile Glu 180 185 190 Val Ala Glu Ile Ile Ala Ser Lys Ser Leu Ile Ala Ala Ala Ala Ala 195 200 205 Lys Asp Ala Val Asn Arg Ala Phe Glu Ser Ser Leu Val Glu Gly Val 210 215 220 Arg Ala Glu Arg Ala Leu Phe Tyr Ser Thr Phe Ala Thr Asp Asp Gln 225 230 235 240 Thr Glu Gly Met Ala Ala Phe Val Glu Lys Arg Asp Pro Asn Phe Thr 245 250 255 His Arg <210> 57 <211> 258 <212> PRT <213> Halalkalicoccus jeotgali <400> 57 Met Ala Asp Arg Val Leu Ile Glu Arg Glu Asn Asp Ile Ala Thr Ile 1 5 10 15 Ile Val Asn Arg Pro Glu Lys Arg Asn Ala Met Asp Ile Pro Thr Arg 20 25 30 Lys Ala Leu Tyr Ala Ala Phe Glu Glu Val Ser Glu Asp Asp Asp Val 35 40 45 Arg Ala Ile Val Leu Arg Gly Ala Gly Asp Gly Ser Phe Ile Ala Gly 50 55 60 Gly Asp Ile Asp Ser Phe Ala Asp Phe Asp His Met Asp Gly Met Glu 65 70 75 80 Tyr Ser Glu Lys Tyr Ala Gln Gly Leu Tyr Asn Tyr Val Ala Asp Arg 85 90 95 His Lys Pro Thr Ile Ala Ala Val Asp Gly Tyr Ala Leu Gly Gly Gly 100 105 110 Thr Glu Ile Ala Leu Ala Cys Asp Ile Arg Leu Ala Thr Asp Asp Ala 115 120 125 Lys Phe Gly Leu Pro Glu Val Gly Ile Gly Val Ile Pro Ala Gly Gly 130 135 140 Gly Thr Gln Arg Leu Val Gln Val Val Gly Ala Gly Leu Ala Ser Glu 145 150 155 160 Leu Ile Leu Thr Gly Arg Ile Ile Ser Ala Asp Glu Ala Lys Arg Ile 165 170 175 Gly Leu Ala Asn His Val Tyr Ala Ala Glu Glu Phe Asp Asn Glu Val 180 185 190 Arg Ala Met Ala Glu Asp Leu Ala Ser Lys Ala Pro Val Ala Gln Arg 195 200 205 Leu Ala Lys Glu Ser Ile Arg Arg Ser Leu Asp Ile Asp Ala Gly Leu 210 215 220 Glu Tyr Glu Arg Leu Ala Gly Ala Phe Leu Phe Gly Thr Asp Asp Gln 225 230 235 240 Lys Glu Gly Ala Asn Ala Phe Leu Glu Asp Arg Glu Pro Lys Tyr Arg 245 250 255 Asn Arg <210> 58 <211> 257 <212> PRT <213> Carboxydothermus hydrogenoformans <400> 58 Met Glu Phe Glu Lys Ile Lys Phe Glu Val Thr Asp Gly Tyr Ala Val 1 5 10 15 Ile Tyr Leu Asn Asn Pro Pro Val Asn Ala Leu Gly Gln Lys Val Leu 20 25 30 Lys Asp Leu Gln Lys Ala Leu Gln Glu Ile Glu Lys Asn Pro Glu Ile 35 40 45 Arg Ala Val Ile Ile Ser Gly Glu Gly Ser Lys Val Phe Cys Ala Gly 50 55 60 Ala Asp Ile Thr Glu Phe Ala Asp Arg Ala Lys Gly Ile Leu Pro Glu 65 70 75 80 Val Glu Gly Ser Val Leu Phe Arg Gln Ile Glu Leu Phe Pro Lys Pro 85 90 95 Val Ile Ala Ala Leu Asn Gly Ser Ser Tyr Gly Gly Gly Thr Glu Leu 100 105 110 Ala Ile Ser Cys His Leu Arg Ile Leu Ala Asp Asp Ala Ser Met Ala 115 120 125 Leu Pro Glu Val Lys Leu Gly Ile Ile Pro Gly Trp Gly Gly Thr Gln 130 135 140 Arg Leu Pro Arg Leu Ile Gly Lys Thr Arg Ala Leu Glu Ala Met Leu 145 150 155 160 Thr Gly Glu Pro Ile Thr Ala Glu Glu Ala Leu Ser Tyr Gly Leu Val 165 170 175 Asn Lys Val Val Pro Lys Asp Gln Val Leu Thr Glu Ala Arg Ala Leu 180 185 190 Ala Ala Lys Leu Ala Lys Gly Ala Pro Ile Ala Met Arg Glu Ile Leu 195 200 205 Lys Ala Val Thr Leu Gly Leu Asp Thr Ser Ile Glu Glu Gly Leu Lys 210 215 220 Ile Glu Lys Glu Gly Ser Lys Val Ala Phe Ser Ser Glu Asp Ala Val 225 230 235 240 Glu Gly Arg Thr Ala Phe Phe Glu Lys Arg Pro Pro Asn Phe Lys Gly 245 250 255 Arg <210> 59 <211> 257 <212> PRT <213> Thermomicrobium roseum <400> 59 Met Ser Val Arg Val Glu Arg Glu Gly Ala Ile Thr Leu Val Thr Val 1 5 10 15 Glu Arg Pro Glu Arg Leu Asn Ala Leu Asp Thr Ala Thr Leu Arg Ala 20 25 30 Leu Leu Ala Ala Val Gln Glu Leu Ala Thr Glu Glu Ala Ile Ala Val 35 40 45 Val Val Leu Thr Gly Ala Gly Asp Arg Ala Phe Ile Ala Gly Ala Asp 50 55 60 Ile Ser Glu Met Val Glu Lys Ser Pro Ala Glu Ala Leu Ala Phe Ala 65 70 75 80 Glu Leu Gly His Ala Val Cys Arg Ala Ile Glu Glu Ala Pro Gln Pro 85 90 95 Tyr Ile Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly Gly Cys Glu Ile 100 105 110 Ala Leu Ala Cys Asp Ile Arg Leu Ala Ser Glu Arg Ala Val Phe Ala 115 120 125 Gln Pro Glu Val Thr Leu Gly Ile Pro Pro Gly Trp Gly Gly Ser Gln 130 135 140 Arg Leu Pro Arg Val Val Pro Pro Gly Ile Ala Arg Glu Leu Leu Tyr 145 150 155 160 Thr Gly Arg Arg Val Asp Ala Gln Glu Ala Leu Arg Ile Gly Leu Val 165 170 175 Asn Ala Val Tyr Pro Ala Asp Gln Leu Leu Glu Arg Ala Arg Glu Leu 180 185 190 Ala Asn Arg Ile Ala Ala Asn Gly Pro Leu Ala Val Arg Leu Thr Lys 195 200 205 Ala Ala Val Arg Phe Gly Leu Glu Gln Gly Leu Glu Ala Gly Leu Thr 210 215 220 Tyr Glu Arg Gln Val Phe Ala Tyr Ala Phe Thr Thr Glu Asp Gln Arg 225 230 235 240 Glu Gly Met Arg Ala Phe Leu Glu Lys Arg Arg Pro Ala Phe Arg Gly 245 250 255 Arg <210> 60 <211> 274 <212> PRT <213> Methylobacterium extorquens <400> 60 Met Asn Ala Asp Ala Glu Thr Ala Ser Thr Asp Glu Leu Leu Phe Ala 1 5 10 15 Val Asp Ala Ala Gly Ile Ala Arg Ile Thr Leu Asn Arg Pro Lys Ala 20 25 30 Arg Asn Ala Leu Thr Phe Ala Met Tyr Arg Gly Leu Val Glu Leu Cys 35 40 45 Glu Arg Ile Glu Ala Asp His Ala Ile Lys Ala Val Ile Ile Thr Gly 50 55 60 Ala Gly Asp Lys Ala Phe Ala Ala Gly Thr Asp Ile Ala Gln Phe Arg 65 70 75 80 Ser Phe Ser Lys Pro Glu Asp Ala Ile Gly Tyr Glu Arg Phe Met Asp 85 90 95 Arg Val Leu Gly Gly Leu Glu Arg Leu Arg Val Pro Thr Ile Ala Ala 100 105 110 Val Ala Gly Ala Cys Thr Gly Gly Gly Ala Ala Ile Ala Ala Ala Cys 115 120 125 Asp Met Arg Ile Ala Ser Arg Asp Ala Arg Phe Gly Ile Pro Ile Ala 130 135 140 Arg Thr Leu Gly Asn Cys Leu Ser Gln Asn Thr Leu Arg Arg Leu Ala 145 150 155 160 Asn Leu Ile Gly Ala Pro Arg Val Lys Asp Ile Leu Phe Thr Ala Arg 165 170 175 Leu Val Glu Ala Gln Glu Ala Leu Ala Ile Gly Leu Val Asn Glu Val 180 185 190 Val Glu Asp Ala Ala Ala Val Ala Ala Arg Ala Asp Ala Leu Ala Thr 195 200 205 Leu Leu Ala Ser His Ala Pro Leu Thr Leu Gln Ala Thr Lys Glu Gly 210 215 220 Leu Arg Arg Ile Gly Glu Glu Gly Ala Ala Glu Ala Ala Glu Gly Glu 225 230 235 240 Arg Pro Gly Asp Asp Leu Ile Val Met Thr Tyr Met Ser Ala Asp Phe 245 250 255 Arg Glu Gly Met Glu Ala Phe Leu Gly Lys Arg Pro Pro Asn Phe Lys 260 265 270 Gly Arg <210> 61 <211> 407 <212> PRT <213> Clostridium sporogenes <400> 61 Met Ser Asp Arg Asn Lys Glu Val Lys Glu Lys Lys Ala Lys His Tyr 1 5 10 15 Leu Arg Glu Ile Thr Ala Lys His Tyr Lys Glu Ala Leu Glu Ala Lys 20 25 30 Glu Arg Gly Glu Lys Val Gly Trp Cys Ala Ser Asn Phe Pro Gln Glu 35 40 45 Ile Ala Thr Thr Leu Gly Val Lys Val Val Tyr Pro Glu Asn His Ala 50 55 60 Ala Ala Val Ala Ala Arg Gly Asn Gly Gln Asn Met Cys Glu His Ala 65 70 75 80 Glu Ala Met Gly Phe Ser Asn Asp Val Cys Gly Tyr Ala Arg Val Asn 85 90 95 Leu Ala Val Met Asp Ile Gly His Ser Glu Asp Gln Pro Ile Pro Met 100 105 110 Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn Gln Met Ile Lys 115 120 125 Trp Tyr Glu His Ile Ala Lys Thr Leu Asp Ile Pro Met Ile Leu Ile 130 135 140 Asp Ile Pro Tyr Asn Thr Glu Asn Thr Val Ser Gln Asp Arg Ile Lys 145 150 155 160 Tyr Ile Arg Ala Gln Phe Asp Asp Ala Ile Lys Gln Leu Glu Glu Ile 165 170 175 Thr Gly Lys Lys Trp Asp Glu Asn Lys Phe Glu Glu Val Met Lys Ile 180 185 190 Ser Gln Glu Ser Ala Lys Gln Trp Leu Arg Ala Ala Ser Tyr Ala Lys 195 200 205 Tyr Lys Pro Ser Pro Phe Ser Gly Phe Asp Leu Phe Asn His Met Ala 210 215 220 Val Ala Val Cys Ala Arg Gly Thr Gln Glu Ala Ala Asp Ala Phe Lys 225 230 235 240 Met Leu Ala Asp Glu Tyr Glu Glu Asn Val Lys Thr Gly Lys Ser Thr 245 250 255 Tyr Arg Gly Glu Glu Lys Gln Arg Ile Leu Phe Glu Gly Ile Ala Cys 260 265 270 Trp Pro Tyr Leu Arg His Lys Leu Thr Lys Leu Ser Glu Tyr Gly Met 275 280 285 Asn Val Thr Ala Thr Val Tyr Ala Glu Ala Phe Gly Val Ile Tyr Glu 290 295 300 Asn Met Asp Glu Leu Met Ala Ala Tyr Asn Lys Val Pro Asn Ser Ile 305 310 315 320 Ser Phe Glu Asn Ala Leu Lys Met Arg Leu Asn Ala Val Thr Ser Thr 325 330 335 Asn Thr Glu Gly Ala Val Ile His Ile Asn Arg Ser Cys Lys Leu Trp 340 345 350 Ser Gly Phe Leu Tyr Glu Leu Ala Arg Arg Leu Glu Lys Glu Thr Gly 355 360 365 Ile Pro Val Val Ser Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe 370 375 380 Ser Glu Ala Gln Tyr Asp Thr Arg Ile Gln Gly Leu Asn Glu Val Met 385 390 395 400 Val Ala Lys Lys Glu Ala Glu 405 <210> 62 <211> 374 <212> PRT <213> Clostridium sporogenes <400> 62 Met Ser Asn Ser Asp Lys Phe Phe Asn Asp Phe Lys Asp Ile Val Glu 1 5 10 15 Asn Pro Lys Lys Tyr Ile Met Lys His Met Glu Gln Thr Gly Gln Lys 20 25 30 Ala Ile Gly Cys Met Pro Leu Tyr Thr Pro Glu Glu Leu Val Leu Ala 35 40 45 Ala Gly Met Phe Pro Val Gly Val Trp Gly Ser Asn Thr Glu Leu Ser 50 55 60 Lys Ala Lys Thr Tyr Phe Pro Ala Phe Ile Cys Ser Ile Leu Gln Thr 65 70 75 80 Thr Leu Glu Asn Ala Leu Asn Gly Glu Tyr Asp Met Leu Ser Gly Met 85 90 95 Met Ile Thr Asn Tyr Cys Asp Ser Leu Lys Cys Met Gly Gln Asn Phe 100 105 110 Lys Leu Thr Val Glu Asn Ile Glu Phe Ile Pro Val Thr Val Pro Gln 115 120 125 Asn Arg Lys Met Glu Ala Gly Lys Glu Phe Leu Lys Ser Gln Tyr Lys 130 135 140 Met Asn Ile Glu Gln Leu Glu Lys Ile Ser Gly Asn Lys Ile Thr Asp 145 150 155 160 Glu Ser Leu Glu Lys Ala Ile Glu Ile Tyr Asp Glu His Arg Lys Val 165 170 175 Met Asn Asp Phe Ser Met Leu Ala Ser Lys Tyr Pro Gly Ile Ile Thr 180 185 190 Pro Thr Lys Arg Asn Tyr Val Met Lys Ser Ala Tyr Tyr Met Asp Lys 195 200 205 Lys Glu His Thr Glu Lys Val Arg Gln Leu Met Asp Glu Ile Lys Ala 210 215 220 Ile Glu Pro Lys Pro Phe Glu Gly Lys Arg Val Ile Thr Thr Gly Ile 225 230 235 240 Ile Ala Asp Ser Glu Asp Leu Leu Lys Ile Leu Glu Glu Asn Asn Ile 245 250 255 Ala Ile Val Gly Asp Asp Ile Ala His Glu Ser Arg Gln Tyr Arg Thr 260 265 270 Leu Thr Pro Glu Ala Asn Thr Pro Met Asp Arg Leu Ala Glu Gln Phe 275 280 285 Ala Asn Arg Glu Cys Ser Thr Leu Tyr Asp Pro Glu Lys Lys Arg Gly 290 295 300 Gln Tyr Ile Val Glu Met Ala Lys Glu Arg Lys Ala Asp Gly Ile Ile 305 310 315 320 Phe Phe Met Thr Lys Phe Cys Asp Pro Glu Glu Tyr Asp Tyr Pro Gln 325 330 335 Met Lys Lys Asp Phe Glu Glu Ala Gly Ile Pro His Val Leu Ile Glu 340 345 350 Thr Asp Met Gln Met Lys Asn Tyr Glu Gln Ala Arg Thr Ala Ile Gln 355 360 365 Ala Phe Ser Glu Thr Leu 370 <210> 63 <211> 264 <212> PRT <213> Clostridium sporogenes <400> 63 Met Ala Asp Ile Tyr Thr Met Gly Val Asp Ile Gly Ser Thr Ala Ser 1 5 10 15 Lys Thr Val Val Leu Lys Asn Gly Lys Glu Ile Val Ser Gln Ala Val 20 25 30 Ile Ser Val Gly Ala Gly Thr Ser Gly Pro Lys Arg Ala Ile Asp Ser 35 40 45 Val Leu Lys Asp Ala Lys Leu Ser Ile Glu Asp Leu Asp Tyr Ile Val 50 55 60 Ser Thr Gly Tyr Gly Arg Asn Ser Phe Asp Phe Ala Asn Lys Gln Ile 65 70 75 80 Ser Glu Leu Ser Cys His Ala Lys Gly Val Tyr Phe Asp Asn Asn Lys 85 90 95 Ala Arg Thr Val Ile Asp Ile Gly Gly Gln Asp Ile Lys Val Leu Lys 100 105 110 Leu Ala Asp Ser Gly Arg Leu Leu Asn Phe Ile Met Asn Asp Lys Cys 115 120 125 Ala Ala Gly Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Ile Glu 130 135 140 Val Pro Val Asp Glu Leu Gly Lys Lys Ala Leu Glu Ser Lys Asn Pro 145 150 155 160 Cys Thr Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile 165 170 175 Ser Gln Leu Ala Arg Gly Val Lys Thr Glu Asp Leu Ile Ala Gly Ile 180 185 190 Cys Lys Ser Val Ala Ser Arg Val Ala Ser Leu Ala Lys Arg Ser Gly 195 200 205 Ile Glu Glu Leu Val Val Met Ser Gly Gly Val Ala Lys Asn Ile Gly 210 215 220 Val Val Lys Ala Met Glu Ala Glu Leu Gly Arg Asp Ile Tyr Ile Ser 225 230 235 240 Lys Asn Ser Gln Leu Asn Gly Ala Leu Gly Ala Ser Leu Tyr Ala Tyr 245 250 255 Glu Ser Phe Gln Lys Glu Arg Ser 260 <210> 64 <211> 412 <212> PRT <213> Clostridium sporogenes <400> 64 Met Glu Asn Asn Thr Asn Met Phe Ser Gly Val Lys Val Ile Glu Leu 1 5 10 15 Ala Asn Phe Ile Ala Ala Pro Ala Ala Gly Arg Phe Phe Ala Asp Gly 20 25 30 Gly Ala Glu Val Ile Lys Ile Glu Ser Pro Ala Gly Asp Pro Leu Arg 35 40 45 Tyr Thr Ala Pro Ser Glu Gly Arg Pro Leu Ser Gln Glu Glu Asn Thr 50 55 60 Thr Tyr Asp Leu Glu Asn Ala Asn Lys Lys Ala Ile Val Leu Asn Leu 65 70 75 80 Lys Ser Glu Lys Gly Lys Lys Ile Leu His Glu Met Leu Ala Glu Ala 85 90 95 Asp Ile Leu Leu Thr Asn Trp Arg Thr Lys Ala Leu Val Lys Gln Gly 100 105 110 Leu Asp Tyr Glu Thr Leu Lys Glu Lys Tyr Pro Lys Leu Val Phe Ala 115 120 125 Gln Ile Thr Gly Tyr Gly Glu Lys Gly Pro Asp Lys Asp Leu Pro Gly 130 135 140 Phe Asp Tyr Thr Ala Phe Phe Ala Arg Gly Gly Val Ser Gly Thr Leu 145 150 155 160 Tyr Glu Lys Gly Thr Val Pro Pro Asn Val Val Pro Gly Leu Gly Asp 165 170 175 His Gln Ala Gly Met Phe Leu Ala Ala Gly Met Ala Gly Ala Leu Tyr 180 185 190 Lys Ala Lys Thr Thr Gly Gln Gly Asp Lys Val Thr Val Ser Leu Met 195 200 205 His Ser Ala Met Tyr Gly Leu Gly Ile Met Ile Gln Ala Ala Gln Tyr 210 215 220 Lys Asp His Gly Leu Val Tyr Pro Ile Asn Arg Asn Glu Thr Pro Asn 225 230 235 240 Pro Phe Ile Val Ser Tyr Lys Ser Lys Asp Asp Tyr Phe Val Gln Val 245 250 255 Cys Met Pro Pro Tyr Asp Val Phe Tyr Asp Arg Phe Met Thr Ala Leu 260 265 270 Gly Arg Glu Asp Leu Val Gly Asp Glu Arg Tyr Asn Lys Ile Glu Asn 275 280 285 Leu Lys Asp Gly Arg Ala Lys Glu Val Tyr Ser Ile Ile Glu Gln Gln 290 295 300 Met Val Thr Lys Thr Lys Asp Glu Trp Asp Asn Ile Phe Arg Asp Ala 305 310 315 320 Asp Ile Pro Phe Ala Ile Ala Gln Thr Trp Glu Asp Leu Leu Glu Asp 325 330 335 Glu Gln Ala Trp Ala Asn Asp Tyr Leu Tyr Lys Met Lys Tyr Pro Thr 340 345 350 Gly Asn Glu Arg Ala Leu Val Arg Leu Pro Val Phe Phe Lys Glu Ala 355 360 365 Gly Leu Pro Glu Tyr Asn Gln Ser Pro Gln Ile Ala Glu Asn Thr Val 370 375 380 Glu Val Leu Lys Glu Met Gly Tyr Thr Glu Gln Glu Ile Glu Glu Leu 385 390 395 400 Glu Lys Asp Lys Asp Ile Met Val Arg Lys Glu Lys 405 410 <210> 65 <211> 368 <212> PRT <213> Lachnoanaerobaculum saburreum <400> 65 Met Trp His Cys Leu Glu Thr Leu Lys Lys Ile Ser Ala Ser Pro Lys 1 5 10 15 Glu Gln Leu Asn Lys Tyr Leu Glu Glu Gly Lys Lys Val Ile Ala Val 20 25 30 Ala Pro Val Tyr Thr Pro Glu Glu Ile Ile His Ala Phe Gly Phe Val 35 40 45 Pro Met Gly Val Trp Gly Ala Asp Ile Glu Ile Asn Glu Ser Lys Lys 50 55 60 Tyr Tyr Pro Ala Phe Ile Cys Ser Ile Met Gln Thr Val Leu Glu Leu 65 70 75 80 Gly Ile Lys Gly Asn Tyr Asn Gly Val Ser Ala Ile Val Val Pro Ser 85 90 95 Leu Cys Asp Ser Leu Lys Thr Leu Gly Gln Asn Trp Lys Tyr Ala Val 100 105 110 Lys Asp Ile Pro Phe Ile Pro Met Thr Tyr Pro Gln Asn Arg Lys Ser 115 120 125 Asp Tyr Ala Val Asp Phe Thr Leu Glu Met Tyr Lys Arg Val Ile Ser 130 135 140 Asp Leu Glu Asn Ile Thr Gly Glu Lys Phe Asp Glu Gly Lys Leu Lys 145 150 155 160 Asn Thr Tyr Glu Ile Tyr Asn Glu His Asn Arg Val Met Arg Glu Phe 165 170 175 Thr Lys Val Ser Glu Glu Tyr Glu Val Ser Ala Thr Asp Arg Ser Ala 180 185 190 Val Phe Lys Ser Ala Trp Phe Met Leu Lys Glu Glu His Thr Glu Leu 195 200 205 Val Arg Glu Leu Ile Glu Leu Ile Lys Lys Glu Gly Lys Ile Ser Lys 210 215 220 Lys Leu Arg Ile Tyr Thr Thr Gly Ile Leu Ala Asp Ala Pro Asp Leu 225 230 235 240 Leu Asn Ile Phe Asp Ser Asn Asn Met Gln Ile Val Gly Asp Asp Ile 245 250 255 Ala Tyr Glu Ser Arg Gln Tyr Arg Thr Asp Ile Pro Asp Gly Asn Gly 260 265 270 Leu Tyr Ala Leu Ala Lys Lys Phe Ser Asn Met Asp Asn Cys Thr Leu 275 280 285 Leu Tyr Asp Lys Asp Lys Arg Arg Val Asp Phe Ile Ile Glu Glu Ala 290 295 300 Lys Lys Lys Arg Ala Asp Gly Ile Val Val Leu Met Thr Lys Phe Cys 305 310 315 320 Asp Pro Glu Glu Phe Asp Tyr Val Pro Ile Lys Arg Ala Ala Asn Glu 325 330 335 Ala Gly Ile Pro His Ile Asn Ile Glu Val Asp Arg Gln Met Lys Asn 340 345 350 Tyr Gln Gln Ala Asn Thr Met Leu Gln Thr Phe Ala Asp Met Leu Val 355 360 365 <210> 66 <211> 409 <212> PRT <213> Lachnoanaerobaculum saburreum <400> 66 Met Glu Glu Ala Lys Lys Gln Lys Pro Thr Val Asp Pro Asn Ser Ala 1 5 10 15 Lys Ala Arg Leu Gly Arg Ile Ala Ala Lys Ala Tyr Ser Asp Cys Val 20 25 30 Glu Ala Lys Lys Arg Gly Glu Leu Val Gly Trp Cys Ala Ser Asn Phe 35 40 45 Pro Val Glu Ile Pro Glu Thr Leu Gly Leu Tyr Val Cys Tyr Pro Glu 50 55 60 Asn Gln Ala Ala Gly Ile Ala Ala Arg Gly Gly Gly Glu Arg Met Cys 65 70 75 80 Ser Glu Ser Glu Gly Asp Gly Tyr Ser Asn Asp Ile Cys Ala Tyr Ala 85 90 95 Arg Ile Ser Leu Ala Tyr Met Lys Leu Lys Glu Ala Pro Glu Gln Asp 100 105 110 Met Pro Gln Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn Cys 115 120 125 Met Ile Lys Trp Tyr Glu Asn Ile Ala Lys Glu Leu Asn Ile Pro Met 130 135 140 Ile Met Ile Asp Ile Pro Phe Asn Pro Asp Tyr Glu Val Ser Asp Ala 145 150 155 160 Met Thr Ala Tyr Ile Arg Asn Gln Phe Trp Asp Ala Ile His Gln Leu 165 170 175 Glu Glu Ile Thr Gly Lys Lys Trp Ser Asn Glu Arg Tyr Glu Glu Val 180 185 190 Arg Lys Ile Ser Gly Arg Ser Ser Arg Ala Trp Leu Glu Ala Thr Ala 195 200 205 Thr Ala Lys Tyr Ser Pro Ser Pro Phe Asn Gly Phe Asp Leu Leu Asn 210 215 220 His Met Ala Val Met Val Thr Ala Arg Gly Lys Leu Glu Ala Ala Glu 225 230 235 240 Ala Met Glu Thr Leu Leu Gln Glu Tyr Lys Asp Asn His Glu Lys Gly 245 250 255 Glu Ser Thr Phe Lys Gly Glu Glu Lys Tyr Arg Ile Met Phe Glu Gly 260 265 270 Ile Ala Cys Trp Pro Trp Leu Arg Ala Thr Ala Thr Gly Leu Lys Ser 275 280 285 Arg Gly Ile Asn Met Val Thr Thr Ile Tyr Ala Asp Ala Phe Gly Phe 290 295 300 Ile Tyr Asp Asp Phe Asp Gly Met Cys Arg Ala Tyr Ala Asn Val Pro 305 310 315 320 Asn Cys Met Asn Ile Glu His Ala Arg Asp Lys Arg Ile Lys Leu Cys 325 330 335 Lys Asp Asn Ser Val Glu Gly Leu Leu Val His Thr Asn Arg Ser Cys 340 345 350 Lys Leu Trp Ser Gly Phe Met Ser Glu Met Ser Arg Gln Ile Gly Glu 355 360 365 Glu Cys Gly Ile Pro Val Val Ser Phe Asp Gly Asp Gln Ala Asp Pro 370 375 380 Arg Asn Phe Ser Glu Ala Gln Tyr Asp Thr Arg Val Gln Gly Leu Thr 385 390 395 400 Glu Ile Met Glu Ala Asn Lys Glu Ile 405 <210> 67 <211> 256 <212> PRT <213> Lachnoanaerobaculum saburreum <400> 67 Met Tyr Thr Leu Gly Val Asp Ile Gly Ser Thr Thr Ser Lys Ala Val 1 5 10 15 Ile Leu Glu Asp Gly Glu Asn Ile Val Ala Ser Ser Ile Val Ile Ala 20 25 30 Thr Val Gly Thr Ala Gly Val Glu Glu Ala Val Lys Asn Val Leu Asn 35 40 45 Phe Ser Lys Leu Glu Leu Asn Asp Ile Lys Ala Val Val Ala Thr Gly 50 55 60 Tyr Gly Arg Met Asn Tyr Asp Val Ala Asp Tyr Lys Val Ser Glu Leu 65 70 75 80 Thr Cys His Ala Leu Gly Val His Lys Glu Phe Pro Asn Val Arg Thr 85 90 95 Val Ile Asp Ile Gly Gly Gln Asp Ala Lys Val Ile Ser Leu Ala Ala 100 105 110 Asn Gly Lys Met Thr Asn Phe Val Met Asn Asp Lys Cys Ala Ala Gly 115 120 125 Thr Gly Arg Phe Leu Asp Val Met Ala Asn Ile Leu Asn Leu Asp Ile 130 135 140 Gln Asp Leu Glu Val Glu Ala Leu Lys Ser Asp Asn Pro Ala Asn Ile 145 150 155 160 Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu 165 170 175 Ala Thr Gly Arg Asn Ile Pro Asp Leu Val Ala Gly Ile Cys Lys Ser 180 185 190 Val Ala Val Arg Val Ala Ala Leu Ala Lys Arg Val Gly Ile Val Glu 195 200 205 Glu Val Cys Met Ser Gly Gly Val Ala Lys Asn Ser Gly Val Arg Asn 210 215 220 Ala Met Ser Lys Glu Leu Gly Val Asp Ile Val Phe Ser Lys Asp Ala 225 230 235 240 Gln Leu Met Gly Ala Leu Gly Ala Ala Ile Tyr Gly Phe Lys Lys Leu 245 250 255 <210> 68 <211> 264 <212> PRT <213> Peptostreptococcus stomatis <400> 68 Met Ser Ser Val Tyr Thr Met Gly Ile Asp Ile Gly Ser Thr Ser Ser 1 5 10 15 Lys Cys Val Ile Met Lys Asp Gly Lys Glu Ile Val Ser Glu Gly Val 20 25 30 Val Ser Leu Gly Ala Gly Thr Lys Gly Ser Asp Leu Val Ile Glu Glu 35 40 45 Val Leu Gly Lys Ala Gly Met Thr Phe Asp Glu Ile Asp Leu Ile Val 50 55 60 Ser Thr Gly Tyr Gly Arg Asn Ser Tyr Glu Arg Ala Ala Lys Thr Val 65 70 75 80 Ser Glu Leu Ser Cys His Ala Lys Gly Gly Gly Tyr Ile Phe Gly Gly 85 90 95 Ala Gly Thr Ile Ile Asp Ile Gly Gly Gln Asp Ile Lys Val Leu Lys 100 105 110 Leu Asn Asp Lys Gly Gly Leu Val Asn Phe Leu Met Asn Asp Lys Cys 115 120 125 Ala Ala Gly Thr Gly Arg Phe Leu Glu Val Met Ser Gly Val Leu Asp 130 135 140 Val Lys Leu Asp Glu Leu Gly Glu Leu Asp Ala Lys Ala Thr Glu Val 145 150 155 160 Thr Pro Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile 165 170 175 Ser Cys Met Ala Lys Lys Ile Pro Leu Glu Asn Ile Ile Arg Gly Ile 180 185 190 His Ala Ser Val Ala Thr Arg Val Ala Ser Leu Ala Arg Arg Gly Gly 195 200 205 Leu Lys Thr Pro Val Ala Met Thr Gly Gly Val Ser Lys Asn Lys Gly 210 215 220 Ile Val Arg Ala Leu Lys Glu Glu Leu Glu Cys Asp Ile Leu Ile Ser 225 230 235 240 Pro Asp Ser Gln Met Ala Gly Ala Ile Gly Ala Ala Leu Tyr Ala Tyr 245 250 255 Asp Glu Tyr Gln Lys Gln Asn Ala 260 <210> 69 <211> 372 <212> PRT <213> Peptostreptococcus stomatis <400> 69 Met Ser Asn Ile Asp Val Leu Leu Gly Lys Leu Asp Val Ser Leu Leu 1 5 10 15 Gly Gln Val Asp Lys Tyr Val Ser Glu Gly Lys Lys Val Ile Gly Cys 20 25 30 Ala Pro Val Tyr Thr Pro Glu Glu Leu Val Tyr Ala Ala Gly Met Val 35 40 45 Pro Ile Gly Val Trp Gly Ala Glu Gly Glu Val Gly Leu Ser Lys Glu 50 55 60 Tyr Phe Pro Ala Phe Tyr Ala Ala Ile Ile Leu Arg Leu Met Asp Leu 65 70 75 80 Gly Leu Glu Gly Lys Leu Asp Lys Met Ser Gly Met Ile Ile Pro Gly 85 90 95 Leu Ser Asp Gly Leu Lys Gly Leu Ser Gln Asn Trp Lys Arg Ala Ile 100 105 110 Lys Gln Val Pro Ala Leu Tyr Ile Gly Tyr Gly Gln Asn Arg Lys Ile 115 120 125 Glu Ala Gly Ile Thr Tyr Asn Glu Lys Gln Tyr Ile Lys Leu Arg Gly 130 135 140 Gln Leu Glu Glu Ile Ala Gly Cys Lys Ile Glu Asp Ala Lys Val Glu 145 150 155 160 Glu Ala Ile Val Leu Tyr Asn Lys His Arg Lys Ala Met Gln Glu Phe 165 170 175 Ser Ser Leu Ala Ala Ser His Leu Asn Thr Ile Thr Pro Ile Leu Arg 180 185 190 Ala Arg Val Met Thr Ser Ala Phe Leu Phe Asp Lys Ala Glu His Leu 195 200 205 Ala Ile Leu Glu Glu Leu Asn Lys Glu Leu Lys Ala Leu Pro Glu Glu 210 215 220 Lys Phe Ala Gly Lys Lys Val Val Thr Thr Gly Ile Leu Ala Asn Ser 225 230 235 240 Pro Gly Met Leu Glu Ile Leu Asp Glu Tyr Lys Leu Gly Ile Val Asp 245 250 255 Asp Asn Ile Asn His Glu Ser Gly Gln Phe Asp Tyr Leu Val Asp Glu 260 265 270 Gly Thr Gly Asn Pro Val Arg Ala Leu Ser Lys Trp Ile Ser Asp Ile 275 280 285 Glu Gly Ser Thr Leu Leu Tyr Asp Pro Glu Lys Leu Arg Gly Gln Ile 290 295 300 Ile Ile Asp Lys Val Lys Lys His Gln Ala Asp Gly Val Ile Tyr Leu 305 310 315 320 Met Thr Lys Phe Ser Asp Ser Asp Glu Phe Asp Tyr Pro Ile Ile Arg 325 330 335 Lys Glu Leu Glu Asn Ala Gly Ile Leu His Ile Leu Val Glu Val Asp 340 345 350 Gln Gln Met Thr Asn Phe Glu Gln Ala Lys Thr Ala Leu Gln Thr Phe 355 360 365 Ala Asp Met Ile 370 <210> 70 <211> 411 <212> PRT <213> Peptostreptococcus stomatis <400> 70 Met Ser Asn Thr Gly Met Val Glu Glu Lys Pro Ala Lys Val Leu Leu 1 5 10 15 Gly Glu Ile Val Ala Lys His Tyr Lys Glu Ala Trp Glu Ala Lys Asn 20 25 30 Asn Gly Glu Leu Val Gly Trp Cys Ala Ser Asn Phe Pro Gln Glu Ile 35 40 45 Phe Glu Thr Met Asp Ile Lys Val Val Tyr Pro Glu Asn Gln Ala Ala 50 55 60 Ala Ile Ser Ala Lys Gly Gly Gly Gln Arg Met Cys Glu Ile Ala Glu 65 70 75 80 Asn Glu Gly Tyr Ser Asn Asp Ile Cys Ala Tyr Ala Arg Ile Ser Leu 85 90 95 Ala Tyr Met Asp Val Lys Asp Ala Pro Glu Leu Asn Met Pro Gln Pro 100 105 110 Asp Phe Val Ala Cys Cys Asn Asn Ile Cys Asn Cys Met Ile Lys Trp 115 120 125 Tyr Glu Asn Ile Ala Lys Glu Leu Asn Ile Pro Leu Ile Leu Ile Asp 130 135 140 Val Pro Tyr Asn Asn Asp Tyr Glu Ala Glu Asp Asp Arg Val Glu Tyr 145 150 155 160 Leu Arg Gly Gln Phe Asp Tyr Ala Ile Lys Gln Leu Glu Glu Leu Thr 165 170 175 Gly Lys Lys Trp Asp Glu Lys Lys Phe Glu Glu Val Met Glu Val Ser 180 185 190 Gln Arg Thr Gly Arg Ala Trp Leu Lys Ala Thr Gly Tyr Ala Lys Tyr 195 200 205 Thr Pro Ser Pro Phe Ser Gly Phe Asp Val Phe Asn His Met Ala Val 210 215 220 Ala Val Cys Ala Arg Gly Lys Ile Glu Ser Ala Ile Ala Phe Glu Lys 225 230 235 240 Leu Ala Glu Glu Phe Asp Glu Asn Val Arg Thr Gly Lys Ser Thr Phe 245 250 255 Lys Gly Glu Glu Lys Phe Arg Val Leu Phe Glu Gly Ile Ala Cys Trp 260 265 270 Pro His Leu Arg His Thr Phe Lys Gln Leu Lys Asp Ala Gly Val Asn 275 280 285 Val Cys Gly Thr Val Tyr Ala Asp Ala Phe Gly Tyr Ile Tyr Asp Asn 290 295 300 Thr Tyr Gln Leu Met Gln Ala Tyr Cys Gly Thr Pro Asn Ala Ile Ser 305 310 315 320 Tyr Glu Arg Ala Thr Asp Met Arg Leu Lys Val Ile Glu Glu Asn Asn 325 330 335 Ile Asp Gly Met Leu Ile His Ile Asn Arg Ser Cys Lys Gln Trp Ser 340 345 350 Gly Ile Met Tyr Glu Met Glu Arg Asp Ile Arg Glu Lys Thr Gly Ile 355 360 365 Pro Thr Ala Thr Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe Ser 370 375 380 Glu Ala Gln Tyr Asp Thr Arg Val Gln Gly Leu Ile Glu Leu Met Glu 385 390 395 400 Ala Asn Lys Ala Ala Lys Met Lys Glu Ala His 405 410 <210> 71 <211> 408 <212> PRT <213> Clostridium difficile <400> 71 Met Ser Glu Lys Lys Glu Ala Arg Val Val Ile Asn Asp Leu Leu Ala 1 5 10 15 Glu Gln Tyr Ala Asn Ala Phe Lys Ala Lys Glu Glu Gly Arg Pro Val 20 25 30 Gly Trp Ser Thr Ser Val Phe Pro Gln Glu Leu Ala Glu Val Phe Asp 35 40 45 Leu Asn Val Leu Tyr Pro Glu Asn Gln Ala Ala Gly Val Ala Ala Lys 50 55 60 Lys Gly Ser Leu Glu Leu Cys Glu Ile Ala Glu Ser Lys Gly Tyr Ser 65 70 75 80 Ile Asp Leu Cys Ala Tyr Ala Arg Thr Asn Phe Gly Leu Leu Glu Asn 85 90 95 Gly Gly Cys Glu Ala Leu Asp Met Pro Ala Pro Asp Phe Leu Leu Cys 100 105 110 Cys Asn Asn Ile Cys Asn Gln Val Ile Lys Trp Tyr Glu Asn Ile Ser 115 120 125 Arg Glu Leu Asp Ile Pro Leu Ile Met Ile Asp Thr Thr Phe Asn Asn 130 135 140 Glu Asp Glu Val Thr Gln Ser Arg Ile Asp Tyr Ile Lys Ala Gln Phe 145 150 155 160 Glu Glu Ala Ile Lys Gln Leu Glu Ile Ile Ser Gly Lys Lys Phe Asp 165 170 175 Pro Lys Lys Phe Glu Glu Val Met Lys Ile Ser Ala Glu Asn Gly Arg 180 185 190 Leu Trp Lys Tyr Ser Met Ser Leu Pro Ala Asp Ser Ser Pro Ser Pro 195 200 205 Met Asn Gly Phe Asp Leu Phe Thr Tyr Met Ala Val Ile Val Cys Ala 210 215 220 Arg Gly Lys Lys Glu Thr Thr Glu Ala Phe Lys Leu Leu Ile Glu Glu 225 230 235 240 Leu Glu Asp Asn Met Lys Thr Gly Lys Ser Ser Phe Arg Gly Glu Glu 245 250 255 Lys Tyr Arg Ile Met Met Glu Gly Ile Pro Cys Trp Pro Tyr Ile Gly 260 265 270 Tyr Lys Met Lys Thr Leu Ala Lys Phe Gly Val Asn Met Thr Gly Ser 275 280 285 Val Tyr Pro His Ala Trp Ala Leu Gln Tyr Glu Val Asn Asp Leu Asp 290 295 300 Gly Met Ala Val Ala Tyr Ser Thr Met Phe Asn Asn Val Asn Leu Asp 305 310 315 320 Arg Met Thr Lys Tyr Arg Val Asp Ser Leu Val Glu Gly Lys Cys Asp 325 330 335 Gly Ala Phe Tyr His Met Asn Arg Ser Cys Lys Leu Met Ser Leu Ile 340 345 350 Gln Tyr Glu Met Gln Arg Arg Ala Ala Glu Glu Thr Gly Leu Pro Tyr 355 360 365 Ala Gly Phe Asp Gly Asp Gln Ala Asp Pro Arg Ala Phe Thr Asn Ala 370 375 380 Gln Phe Glu Thr Arg Ile Gln Gly Leu Val Glu Val Met Glu Glu Arg 385 390 395 400 Lys Lys Leu Asn Arg Gly Glu Ile 405 <210> 72 <211> 375 <212> PRT <213> Clostridium difficile <400> 72 Met Glu Ala Ile Leu Ser Lys Met Lys Glu Val Val Glu Asn Pro Asn 1 5 10 15 Ala Ala Val Lys Lys Tyr Lys Ser Glu Thr Gly Lys Lys Ala Ile Gly 20 25 30 Cys Phe Pro Val Tyr Cys Pro Glu Glu Ile Ile His Ala Ala Gly Met 35 40 45 Leu Pro Val Gly Ile Trp Gly Gly Gln Thr Glu Leu Asp Leu Ala Lys 50 55 60 Gln Tyr Phe Pro Ala Phe Ala Cys Ser Ile Met Gln Ser Cys Leu Glu 65 70 75 80 Tyr Gly Leu Lys Gly Ala Tyr Asp Glu Leu Ser Gly Val Ile Ile Pro 85 90 95 Gly Met Cys Asp Thr Leu Ile Cys Leu Gly Gln Asn Trp Lys Ser Ala 100 105 110 Val Pro His Ile Lys Tyr Ile Ser Leu Val His Pro Gln Asn Arg Lys 115 120 125 Leu Glu Ala Gly Val Lys Tyr Leu Ile Ser Glu Tyr Lys Gly Val Lys 130 135 140 Arg Glu Leu Glu Glu Ile Cys Gly Tyr Glu Ile Glu Glu Ala Lys Ile 145 150 155 160 His Glu Ser Ile Glu Val Tyr Asn Glu His Arg Lys Thr Met Arg Asp 165 170 175 Phe Val Glu Val Ala Tyr Lys His Ser Asn Thr Ile Lys Pro Ser Ile 180 185 190 Arg Ser Leu Val Ile Lys Ser Gly Phe Phe Met Arg Lys Glu Glu His 195 200 205 Thr Glu Leu Val Lys Asp Leu Ile Ala Lys Leu Asn Ala Met Pro Glu 210 215 220 Glu Val Cys Ser Gly Lys Lys Val Leu Leu Thr Gly Ile Leu Ala Asp 225 230 235 240 Ser Lys Asp Ile Leu Asp Ile Leu Glu Asp Asn Asn Ile Ser Val Val 245 250 255 Ala Asp Asp Leu Ala Gln Glu Thr Arg Gln Phe Arg Thr Asp Val Pro 260 265 270 Ala Gly Asp Asp Ala Leu Glu Arg Leu Ala Arg Gln Trp Ser Asn Ile 275 280 285 Glu Gly Cys Ser Leu Ala Tyr Asp Pro Lys Lys Lys Arg Gly Ser Leu 290 295 300 Ile Val Asp Glu Val Lys Lys Lys Asp Ile Asp Gly Val Ile Phe Cys 305 310 315 320 Met Met Lys Phe Cys Asp Pro Glu Glu Tyr Asp Tyr Pro Leu Val Arg 325 330 335 Lys Asp Ile Glu Asp Ser Gly Ile Pro Thr Leu Tyr Val Glu Ile Asp 340 345 350 Gln Gln Thr Gln Asn Asn Glu Gln Ala Arg Thr Arg Ile Gln Thr Phe 355 360 365 Ala Glu Met Met Ser Leu Ala 370 375 <210> 73 <211> 266 <212> PRT <213> Clostridium difficile <400> 73 Met Tyr Thr Met Gly Leu Asp Ile Gly Ser Thr Ala Ser Lys Gly Val 1 5 10 15 Ile Leu Lys Asn Gly Glu Asp Ile Val Ala Ser Glu Thr Ile Ser Ser 20 25 30 Gly Thr Gly Thr Thr Gly Pro Ser Arg Val Leu Glu Lys Leu Tyr Gly 35 40 45 Lys Thr Gly Leu Ala Arg Glu Asp Ile Lys Lys Val Val Val Thr Gly 50 55 60 Tyr Gly Arg Met Asn Tyr Ser Asp Ala Asp Lys Gln Ile Ser Glu Leu 65 70 75 80 Ser Cys His Ala Arg Gly Val Asn Phe Ile Ile Pro Glu Thr Arg Thr 85 90 95 Ile Ile Asp Ile Gly Gly Gln Asp Ala Lys Val Leu Lys Leu Asp Asn 100 105 110 Asn Gly Arg Leu Leu Asn Phe Leu Met Asn Asp Lys Cys Ala Ala Gly 115 120 125 Thr Gly Arg Phe Leu Asp Val Met Ala Lys Ile Ile Glu Val Asp Val 130 135 140 Ser Glu Leu Gly Ser Ile Ser Met Asn Ser Gln Asn Glu Val Ser Ile 145 150 155 160 Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser His Leu 165 170 175 Ser Glu Asn Ala Lys Ile Glu Asp Ile Val Ala Gly Ile His Thr Ser 180 185 190 Val Ala Lys Arg Val Ser Ser Leu Val Lys Arg Ile Gly Val Gln Arg 195 200 205 Asn Val Val Met Val Gly Gly Val Ala Arg Asn Ser Gly Ile Val Arg 210 215 220 Ala Met Ala Arg Glu Ile Asn Thr Glu Ile Ile Val Pro Asp Ile Pro 225 230 235 240 Gln Leu Thr Gly Ala Leu Gly Ala Ala Leu Tyr Ala Phe Asp Glu Ala 245 250 255 Lys Glu Ser Gln Lys Glu Val Lys Asn Ile 260 265 <210> 74 <211> 399 <212> PRT <213> Clostridium difficile <400> 74 Met Leu Leu Glu Gly Val Lys Val Val Glu Leu Ser Ser Phe Ile Ala 1 5 10 15 Ala Pro Cys Cys Ala Lys Met Leu Gly Asp Trp Gly Ala Glu Val Ile 20 25 30 Lys Ile Glu Pro Ile Glu Gly Asp Gly Ile Arg Val Met Gly Gly Thr 35 40 45 Phe Lys Ser Pro Ala Ser Asp Asp Glu Asn Pro Met Phe Glu Leu Glu 50 55 60 Asn Gly Asn Lys Lys Gly Val Ser Ile Asn Val Lys Ser Lys Glu Gly 65 70 75 80 Val Glu Ile Leu His Lys Leu Leu Ser Glu Ala Asp Ile Phe Val Thr 85 90 95 Asn Val Arg Val Gln Ala Leu Glu Lys Met Gly Ile Ala Tyr Asp Gln 100 105 110 Ile Lys Asp Lys Tyr Pro Gly Leu Ile Phe Ser Gln Ile Leu Gly Tyr 115 120 125 Gly Glu Lys Gly Pro Leu Lys Asp Lys Pro Gly Phe Asp Tyr Thr Ala 130 135 140 Tyr Phe Ala Arg Gly Gly Val Ser Gln Ser Val Met Glu Lys Gly Thr 145 150 155 160 Ser Pro Ala Asn Thr Ala Ala Gly Phe Gly Asp His Tyr Ala Gly Leu 165 170 175 Ala Leu Ala Ala Gly Ser Leu Ala Ala Leu His Lys Lys Ala Gln Thr 180 185 190 Gly Lys Gly Glu Arg Val Thr Val Ser Leu Phe His Thr Ala Ile Tyr 195 200 205 Gly Met Gly Thr Met Ile Thr Thr Ala Gln Tyr Gly Asn Glu Met Pro 210 215 220 Leu Ser Arg Glu Asn Pro Asn Ser Pro Leu Met Thr Thr Tyr Lys Cys 225 230 235 240 Lys Asp Gly Arg Trp Ile Gln Leu Ala Leu Ile Gln Tyr Asn Lys Trp 245 250 255 Leu Gly Lys Phe Cys Lys Val Ile Asn Arg Glu Tyr Ile Leu Glu Asp 260 265 270 Asp Arg Tyr Asn Asn Ile Asp Ser Met Val Asn His Val Glu Asp Leu 275 280 285 Val Lys Ile Val Gly Glu Ala Met Leu Glu Lys Thr Leu Asp Glu Trp 290 295 300 Ser Ala Leu Leu Glu Glu Ala Asp Leu Pro Phe Glu Lys Ile Gln Ser 305 310 315 320 Cys Glu Asp Leu Leu Asp Asp Glu Gln Ala Trp Ala Asn Asp Phe Leu 325 330 335 Phe Lys Lys Thr Tyr Asp Ser Gly Asn Thr Gly Val Leu Val Asn Thr 340 345 350 Pro Val Met Phe Arg Asn Glu Gly Ile Lys Glu Tyr Thr Pro Ala Pro 355 360 365 Lys Val Gly Gln His Thr Val Glu Val Leu Lys Ser Leu Gly Tyr Asp 370 375 380 Glu Glu Lys Ile Asn Asn Phe Lys Asp Ser Lys Val Val Arg Tyr 385 390 395 <210> 75 <211> 255 <212> PRT <213> Escherichia coli (strain K12) <400> 75 Met Ser Glu Leu Ile Val Ser Arg Gln Gln Arg Val Leu Leu Leu Thr 1 5 10 15 Leu Asn Arg Pro Ala Ala Arg Asn Ala Leu Asn Asn Ala Leu Leu Thr 20 25 30 Gln Leu Val Asn Glu Leu Glu Ala Ala Ala Ile Asp Thr Ser Ile Ser 35 40 45 Val Cys Val Ile Thr Gly Asn Ala Arg Phe Phe Ala Ala Gly Ala Asp 50 55 60 Leu Asn Glu Met Ala Glu Lys Asp Leu Ala Ala Thr Leu Asn Asp Thr 65 70 75 80 Arg Pro Gln Leu Trp Ala Arg Leu Gln Ala Phe Asn Lys Pro Leu Ile 85 90 95 Ala Ala Val Asn Gly Tyr Ala Leu Gly Ala Gly Cys Glu Leu Ala Leu 100 105 110 Leu Cys Asp Val Val Val Ala Gly Glu Asn Ala Arg Phe Gly Leu Pro 115 120 125 Glu Ile Thr Leu Gly Ile Met Pro Gly Ala Gly Gly Thr Gln Arg Leu 130 135 140 Ile Arg Ser Val Gly Lys Ser Leu Ala Ser Lys Met Val Leu Ser Gly 145 150 155 160 Glu Ser Ile Thr Ala Arg Gln Ala Gln Gln Ala Gly Leu Val Ser Asp 165 170 175 Val Phe Pro Ser Asp Leu Thr Leu Glu Tyr Ala Leu Gln Leu Ala Ser 180 185 190 Lys Met Ala Arg His Ser Pro Leu Ala Leu Gln Ala Ala Lys Gln Ala 195 200 205 Leu Arg Gln Ser Gln Glu Val Ala Leu Gln Ala Gly Leu Ala Gln Glu 210 215 220 Arg Gln Leu Phe Thr Leu Leu Ala Ala Thr Glu Asp Arg His Glu Gly 225 230 235 240 Ile Ser Ala Phe Leu Gln Lys Arg Ser Pro Asp Phe Lys Gly Arg 245 250 255 <210> 76 <211> 257 <212> PRT <213> Rhodobacter capsulatus <400> 76 Met Ser Tyr His Thr Ile Arg Tyr Glu Ile Ser Glu Gly Leu Ala Val 1 5 10 15 Ile Thr Leu Asp Arg Pro Glu Val Met Asn Ala Leu Asn Ala Ala Met 20 25 30 Arg His Glu Leu Thr Ala Ala Leu His Arg Ala Arg Gly Glu Ala Arg 35 40 45 Ala Ile Val Leu Thr Gly Ser Gly Arg Ala Phe Cys Ser Gly Gln Asp 50 55 60 Leu Gly Asp Gly Ala Ala Glu Gly Leu Asn Leu Glu Thr Val Leu Arg 65 70 75 80 Glu Glu Tyr Glu Pro Leu Leu Gln Ala Ile Tyr Ser Cys Pro Leu Pro 85 90 95 Val Leu Ala Ala Val Asn Gly Ala Ala Ala Gly Ala Gly Ala Asn Leu 100 105 110 Ala Leu Ala Ala Asp Val Val Ile Ala Ala Gln Ser Ala Ala Phe Met 115 120 125 Gln Ala Phe Thr Arg Ile Gly Leu Met Pro Asp Ala Gly Gly Thr Trp 130 135 140 Trp Leu Pro Arg Gln Val Gly Met Ala Arg Ala Met Gly Met Ala Leu 145 150 155 160 Phe Ala Glu Lys Ile Gly Ala Glu Glu Ala Ala Arg Met Gly Leu Ile 165 170 175 Trp Glu Ala Val Pro Asp Val Asp Phe Glu His His Trp Arg Ala Arg 180 185 190 Ala Ala His Leu Ala Arg Gly Pro Ser Ala Ala Phe Ala Ala Val Lys 195 200 205 Lys Ala Phe His Ala Gly Leu Ser Asn Pro Leu Pro Ala Gln Leu Ala 210 215 220 Leu Glu Ala Arg Leu Gln Gly Glu Leu Gly Gln Ser Ala Asp Phe Arg 225 230 235 240 Glu Gly Val Gln Ala Phe Leu Glu Lys Arg Pro Pro His Phe Thr Gly 245 250 255 Arg <210> 77 <211> 701 <212> PRT <213> Pseudomonas stutzeri <400> 77 Met Thr Asp Val Ile Arg Leu Glu Arg Arg Gly Asp Ile Ala Leu Ile 1 5 10 15 Leu Val Asn Asn Pro Pro Val Asn Ala Leu Gly His Ala Val Arg Lys 20 25 30 Gly Leu Leu Asp Ala Phe Gln Glu Ala Asp Glu Ala Pro Glu Val Thr 35 40 45 Ala Val Val Leu Val Cys Glu Gly Pro Thr Phe Met Ala Gly Ala Asp 50 55 60 Ile Lys Glu Phe Gly Lys Pro Pro Gln Ala Pro Ser Leu Pro Glu Val 65 70 75 80 Ile Glu Val Ile Glu Gly Cys Arg Lys Pro Ser Val Ala Val Ile His 85 90 95 Gly Thr Ala Leu Gly Gly Gly Leu Glu Val Ala Leu Gly Cys His Tyr 100 105 110 Arg Ile Ala Arg Ser Asp Ala Lys Val Gly Leu Pro Glu Val Lys Leu 115 120 125 Gly Leu Leu Pro Gly Ala Gly Gly Thr Gln Arg Leu Pro Arg Leu Ala 130 135 140 Gly Val Glu Lys Ala Leu Glu Met Ile Val Ser Gly Gln Pro Ile Gly 145 150 155 160 Ala Ala Glu Ala Leu Glu His Tyr Ile Val Asp Glu Leu Phe Glu Gly 165 170 175 Asp Leu Ile Glu Ala Gly Leu Thr Tyr Ala Arg Arg Leu Val Glu Glu 180 185 190 Gly Arg Gly Pro Arg Arg Ser Gly Glu Gln Thr Arg Gly Leu Glu Gly 195 200 205 Val Asp Asn Glu Ala Leu Ile Arg Ala Lys His Ala Glu Val Ala Lys 210 215 220 Arg Met Pro Gly Leu Phe Ser Pro Leu Arg Cys Ile Ala Ala Val Glu 225 230 235 240 Ala Ala Thr Arg Leu Pro Leu Ala Glu Gly Leu Lys Arg Glu Arg Glu 245 250 255 Leu Phe Thr Glu Cys Leu Asn Ser Pro Gln Arg Gly Ala Leu Ile His 260 265 270 Ser Phe Phe Ala Glu Arg Gln Ala Gly Lys Ile Asp Asp Leu Pro Ser 275 280 285 Asp Val Thr Pro Arg Pro Ile Arg Thr Ala Ala Val Ile Gly Gly Gly 290 295 300 Thr Met Gly Val Gly Ile Ala Leu Ser Phe Ala Asn Ala Gly Val Pro 305 310 315 320 Val Lys Leu Leu Glu Ile Asn Asp Glu Ala Leu Gln Arg Gly Leu Gln 325 330 335 Arg Ala Arg Glu Thr Tyr Ala Ala Ser Val Lys Arg Gly Ser Leu Thr 340 345 350 Glu Asp Ala Met Glu Gln Arg Leu Ala Leu Ile Ala Gly Val Thr Asp 355 360 365 Tyr Gly Ala Leu Ala Asp Ala Asp Val Val Val Glu Ala Val Phe Glu 370 375 380 Glu Met Gly Val Lys Gln Gln Val Phe Glu Gln Leu Asp Ala Val Cys 385 390 395 400 Lys Pro Gly Ala Ile Leu Ala Ser Asn Thr Ser Ser Leu Asp Leu Asn 405 410 415 Ala Ile Ala Gly Phe Thr Arg Arg Pro Glu Asp Val Val Gly Met His 420 425 430 Phe Phe Ser Pro Ala Asn Val Met Arg Leu Leu Glu Val Val Arg Gly 435 440 445 Glu Arg Thr Ser Asp Glu Val Leu Ala Ala Ala Met Ala Ile Gly Lys 450 455 460 Gln Leu Lys Lys Val Ser Val Val Val Gly Val Cys Asp Gly Phe Val 465 470 475 480 Gly Asn Arg Met Val Phe Gln Tyr Gly Arg Glu Ala Glu Phe Leu Leu 485 490 495 Glu Glu Gly Ala Thr Pro Gln Gln Val Asp Ala Ala Leu Arg Asn Phe 500 505 510 Gly Met Ala Met Gly Pro Phe Ala Met Arg Asp Leu Ser Gly Leu Asp 515 520 525 Ile Gly Gln Ala Ile Arg Lys Arg Gln Arg Ala Thr Leu Pro Ala His 530 535 540 Leu Asp Phe Pro Thr Val Ser Asp Lys Leu Cys Ala Ala Gly Met Leu 545 550 555 560 Gly Gln Lys Thr Gly Ala Gly Tyr Tyr Arg Tyr Glu Pro Gly Asn Arg 565 570 575 Thr Pro Gln Glu Asn Pro Asp Leu Ala Pro Met Leu Glu Ala Ala Ser 580 585 590 Arg Glu Lys Gly Ile Glu Arg Gln Ala Leu Asp Glu Gln Tyr Ile Val 595 600 605 Glu Arg Cys Ile Phe Ala Leu Val Asn Glu Gly Ala Lys Ile Leu Glu 610 615 620 Glu Gly Ile Ala Gln Arg Ser Ser Asp Ile Asp Val Ile Tyr Leu Asn 625 630 635 640 Gly Tyr Gly Phe Pro Ala Phe Arg Gly Gly Pro Met Tyr Tyr Ala Asp 645 650 655 Ser Val Gly Leu Asp Lys Val Leu Ala Arg Val Lys Glu Leu His Ala 660 665 670 Arg Cys Gly Asp Trp Trp Lys Pro Ala Pro Leu Leu Glu Lys Leu Ala 675 680 685 Ala Glu Gly Arg Thr Phe Thr Glu Trp Gln Ala Gly Gln 690 695 700 <210> 78 <211> 655 <212> PRT <213> Haliangium ochraceum <400> 78 Met Ile Val Gly Val Ile Gly Ser Gly Ala Ile Gly Pro Asp Leu Ala 1 5 10 15 Tyr Gly Phe Ala Ser Ala Leu Ala Ser Val Pro Gly Ala Arg Val Tyr 20 25 30 Leu His Asp Ile Lys Gln Glu Ala Leu Asp Ala Gly Met Gln Arg Ile 35 40 45 Arg Gly Tyr Ile Ala Lys Gly Leu Ala Arg Gly Lys Ile Ser Glu Arg 50 55 60 Val Ala Gly Ala Leu Glu Thr Val Leu Val Pro Thr Leu Ser Leu Ala 65 70 75 80 Asp Leu Ala Pro Cys Ser Tyr Val Leu Glu Ala Ala Thr Glu Glu Leu 85 90 95 Gly Val Lys Arg Ala Ile Leu Arg Ser Leu Glu Asp Thr Val Asp Ser 100 105 110 Glu Cys Leu Ile Gly Phe Ala Thr Ser Gly Leu Pro Arg Ala Ile Ile 115 120 125 Ala Ala Glu Val Lys His Pro Glu Arg Cys Phe Val Asn His Pro Phe 130 135 140 Tyr Pro Ala Trp Arg Ser Leu Pro Val Glu Val Val Leu Ser Gly Ser 145 150 155 160 Pro Ala His Gly Gln Arg Met Leu Ala Thr Leu Glu Ala Leu Gly Lys 165 170 175 Val Pro Val Ile Thr Ala Asp Ala Pro Cys Phe Ala Ala Asp Asp Ile 180 185 190 Phe Cys Asn Tyr Cys Ser Glu Ala Ala Arg Ile Val Glu Glu Gly Ile 195 200 205 Ala Asn Pro Ala Gln Val Asp Ala Ile Val His Gly Ala Ile Gly Gly 210 215 220 Gly Gly Pro Leu Asn Val Leu Asp Ala Thr Arg Gly Asn Leu Leu Thr 225 230 235 240 Val His Cys Gln Glu Leu Met Arg Asp Ala Asp Thr Gly Thr Pro Trp 245 250 255 Phe Glu Pro Pro Ala Ile Leu Arg Glu Arg Gly Asp Ala Leu Trp His 260 265 270 Asp Pro Lys Ala Pro His Asp Pro Ala Phe Asp Glu Ala Leu Arg Glu 275 280 285 Arg Val Leu Asp Arg Ile Leu Ala Val Leu Leu Ala Arg Thr Val Phe 290 295 300 Val Leu Asp His Gly Ile Cys Ala Ala Thr Glu Leu Asp Trp Met Thr 305 310 315 320 Arg Thr Ala Leu Gly Phe Arg Thr Gly Leu Val Asp Leu Val Asp Glu 325 330 335 Leu Gly Pro Glu Arg Val Ala Glu Leu Cys Gln Arg Tyr Ala Ala Glu 340 345 350 His Pro Gly Phe Val Ile Pro Asp Ser Ile Arg Glu Gln His Lys Pro 355 360 365 Arg Phe Tyr Gly Asn Leu Arg Val Thr Arg Gln Asp Glu Leu Ala Ile 370 375 380 Val Arg Ile Phe Arg Pro Glu Val Lys Asn Ala Leu Asp Arg Arg Thr 385 390 395 400 Leu Ser Glu Leu Asp His Leu Met Ala Ala Leu Ser Ala Asp Asp Ser 405 410 415 Val Glu Gly Val Val Leu Ser Ser Ala Gly Gly Ala Leu Ala Gly Ala 420 425 430 Asp Ile Thr Glu Leu Ala Arg Val Arg Thr Thr Glu Glu Ala Val Ser 435 440 445 Thr Cys Ala Phe Gly Gln Ala Val Leu Asn Arg Ile Ala Ala Met Asp 450 455 460 Lys Pro Val Val Ala Ala Val Asp Gly Pro Val Leu Gly Gly Gly Ala 465 470 475 480 Glu Leu Ser Met Ala Cys His Ala Arg Val Val Gly Pro Arg Leu Ser 485 490 495 Met Gly Gln Pro Glu Val Asn Leu Gly Ile Ile Pro Gly Tyr Gly Gly 500 505 510 Thr Gln Arg Leu Pro Arg Leu Ile Gly Val Glu Arg Ala Leu Ala Met 515 520 525 Met Arg Thr Ala Gln Ser Ile Asp Ala Gln Thr Ala Cys Glu Trp Gly 530 535 540 Trp Ala Ser Gly Thr Pro Met Val Asp Phe Val Gly Ala Ala Ala Thr 545 550 555 560 Leu Ile Arg Ser His Leu Ala Gly Glu Ala Glu Leu Ala Pro Leu Asp 565 570 575 Pro Ala Pro Met Ser Val Pro Ala Ala Ala Ala Pro Val Asp Ile Gly 580 585 590 His Arg Ser Arg Val Ile Asp Glu Ile Leu Val Asp Val Val Gln Ser 595 600 605 Gly Leu Arg Ala Pro Leu Ser Glu Gly Leu Ala Thr Glu Ala Ala Gly 610 615 620 Phe Gly Arg Cys Val Leu Thr Val Asp Leu Asp Ile Gly Leu Lys Asn 625 630 635 640 Phe Met Gln Asn Gly Pro Arg Val Pro Ala Leu Phe Leu His Glu 645 650 655 <210> 79 <211> 255 <212> PRT <213> Anoxybacillus flavithermus <400> 79 Met Phe Ser Ile Gln Gln Glu Gly Tyr Val Ala Ile Leu Ala Leu His 1 5 10 15 Arg Pro Pro Ala Asn Ala Leu Ala Ser Ser Val Leu Lys Glu Leu Ser 20 25 30 Glu Arg Leu Asp Ala Leu Lys Glu Asp Glu Gln Val Arg Val Ile Val 35 40 45 Leu His Gly Glu Gly Arg Phe Phe Ser Ala Gly Ala Asp Ile Lys Glu 50 55 60 Phe Thr Ala Ile Glu Ala Ser Glu Gln Ala Ala Glu Leu Ala Arg Ala 65 70 75 80 Gly Gln Gln Val Met Glu Lys Ile Glu Gln Phe Pro Lys Pro Ile Ile 85 90 95 Ala Ala Ile His Gly Ala Ala Leu Gly Gly Gly Leu Glu Leu Ala Met 100 105 110 Ser Cys His Leu Arg Ile Val Ala Glu Asn Ala Lys Leu Gly Leu Pro 115 120 125 Glu Leu Gln Leu Gly Ile Ile Pro Gly Phe Ala Gly Thr Gln Arg Leu 130 135 140 Leu Arg His Val Gly Met Ala Lys Ala Leu Glu Met Met Trp Thr Ser 145 150 155 160 Glu Pro Ile Thr Gly Ala Glu Ala Val Gln Trp Gly Leu Ala Asn Lys 165 170 175 Ala Val Pro Glu Glu Gln Leu Leu Asp Thr Ala Lys Gln Leu Ala Gln 180 185 190 Lys Ile Ala Gln Lys Ser Pro Ile Ser Val Gln Ala Val Leu Lys Leu 195 200 205 Val Asn Glu Ala Arg Thr Lys Thr Phe His Glu Cys Val Glu Lys Glu 210 215 220 Ala Gln Leu Phe Gly Gln Val Phe Val Thr Glu Asp Ala Lys Glu Gly 225 230 235 240 Ile Ser Ala Phe Ile Glu Lys Arg Thr Pro Gln Phe Gln Gly Lys 245 250 255 <210> 80 <211> 260 <212> PRT <213> Streptomyces avermitilis <400> 80 Met Ser Thr Ala Pro Glu Ala Ala Asp Leu Val Leu His Glu Arg His 1 5 10 15 Gly Gly Val Leu Thr Ile Thr Ile Asn Arg Pro Ala Gln Lys Asn Ala 20 25 30 Val Asp His Glu Ala Ala Val Gln Leu Ala Ala Ala Val Asp Leu Leu 35 40 45 Asp Ala Asp Pro Glu Leu Ser Val Gly Val Leu Thr Gly Ala Gly Gly 50 55 60 Val Phe Ser Ala Gly Met Asp Leu Lys Ala Phe Ala Lys Gly Glu Leu 65 70 75 80 Pro Leu Leu Pro Ser Arg Gly Leu Gly Gly Leu Thr Arg Ala Ser Val 85 90 95 Arg Lys Pro Leu Val Ala Ala Val Glu Gly Trp Ala Leu Gly Gly Gly 100 105 110 Phe Glu Leu Val Leu Ala Cys Asp Leu Ile Val Ala Ala Glu Asp Ala 115 120 125 Arg Phe Gly Phe Pro Glu Val Met Arg Gly Leu Val Ala Ala Glu Gly 130 135 140 Gly Leu Val Arg Leu Pro Arg Arg Leu Pro Tyr His Val Ala Ala Arg 145 150 155 160 Val Leu Leu Thr Gly Glu Pro Leu Thr Ala Val Glu Ala Lys Glu Tyr 165 170 175 Gly Leu Val Asn Glu Leu Thr Pro Pro Gly Ala Ala Leu Asp Ala Ala 180 185 190 Arg Glu Leu Ala Gly Arg Val Ala Arg Asn Ala Pro Leu Ala Leu Ala 195 200 205 Ala Val Lys Glu Val Leu Arg Glu Thr Gln Gly Leu Lys Glu Ser Asp 210 215 220 Ala Phe Arg Arg Gln Asp Glu Leu Thr Ser Gly Leu Ala Ala Ser Glu 225 230 235 240 Asp Ala Arg Glu Gly Ala Gln Ala Phe Ala Glu Lys Arg Ala Pro Val 245 250 255 Trp His Gly Arg 260 <210> 81 <211> 560 <212> PRT <213> Advenella kashmirensis <400> 81 Met Asp Asn Gly Arg Lys Leu Ile Glu Arg Gly Trp His Leu Phe Asn 1 5 10 15 Arg Ile Glu Lys Leu Ala Phe Pro Thr Leu Ala Leu Met His Gly Pro 20 25 30 Cys Leu Gly Gly Gly Leu Glu Leu Ala Leu Ala Cys Arg Tyr Arg Ile 35 40 45 Ala Ile Asp Ser Pro Lys Pro Val Ile Gly Leu Pro Glu Val Lys Leu 50 55 60 Gly Ile Phe Pro Ala Trp Gly Gly Leu Met Arg Leu Pro Arg Leu Ile 65 70 75 80 Gly Pro Gln Thr Ala Leu Asn Met Met Leu Thr Gly Arg Thr Leu Asp 85 90 95 Gly Arg Lys Ala Arg Ser Ala Gly Leu Val Asp Leu Leu Val Ala Pro 100 105 110 Arg Val Ala Glu Lys Ser Ala Ile Asp Leu Val Thr Ser Gly Lys Pro 115 120 125 Ala Arg Gln Ala Arg Gly Leu Ala Gly Leu Leu Asn Arg Ala Pro Phe 130 135 140 Lys Ser Leu Val Ala Ala Gln Ala Arg Lys Ser Val Lys Gln Lys Asp 145 150 155 160 Pro Tyr Gly His Tyr Pro Ala Thr Leu Thr Met Leu Asp Leu Trp Glu 165 170 175 Lys His Asp Gly Asp Pro Leu Ala Asp Pro Gln Ala Leu Thr Arg Leu 180 185 190 Leu Gln Ser Asp Val Thr Arg Asn Leu Ile Arg Val Phe His Leu Gln 195 200 205 Glu Arg Leu Lys Ala Phe Gly Lys Lys Asp Asn Ala Thr Pro Val Asn 210 215 220 His Val His Val Ile Gly Ala Gly Val Met Gly Gly Gly Ile Ala Ala 225 230 235 240 Trp Cys Ala Leu Gln Gly Ile Lys Thr Thr Leu Gln Asp Thr Asp Ala 245 250 255 Gln Arg Ile Ala Gly Ala Phe Lys Asn Ala Val Ser Ile Tyr Ala Arg 260 265 270 Lys Asp Arg Tyr Thr Ala Gln Ala Ala Arg Asp Arg Leu Ile Pro Asp 275 280 285 Leu Ala Gly His Gly Ile Ala Thr Ala Asp Leu Val Ile Glu Ala Ile 290 295 300 Ser Glu Asn Pro Gln Ala Lys Gln Ser Leu Tyr Gln Gln Ile Glu Pro 305 310 315 320 Lys Met Lys Glu Gly Ala Ile Leu Ala Thr Asn Thr Ser Ser Leu Ser 325 330 335 Ile Ala Gln Leu Arg Ser Val Leu Val His Pro Glu Arg Phe Val Gly 340 345 350 Ile His Phe Phe Asn Pro Val Ser Arg Met Pro Leu Val Glu Val Val 355 360 365 His Ala Asp Gly Ile Ala Gln Glu Thr Leu Asp Thr Ala Ala Ala Phe 370 375 380 Val Gly Lys Ile Gly Lys Leu Pro Leu Pro Val Gln Asp Thr Pro Gly 385 390 395 400 Phe Leu Val Asn Ala Val Leu Ala Pro Tyr Met Leu Gln Ala Met Arg 405 410 415 Cys Ile Asp Glu Gly Met Asp Pro Glu Val Ile Asp Thr Ala Met Leu 420 425 430 Glu Phe Gly Met Pro Met Gly Pro Ile Thr Leu Ala Asp Thr Val Gly 435 440 445 Leu Asp Ile Ala Met Ala Ala Gly Lys Gln Leu Ser Glu Gly Gln Glu 450 455 460 Pro Pro Arg Cys Leu Gln Glu Lys Ile Ala Gln Gly Lys Leu Gly Val 465 470 475 480 Lys Ser Gly Glu Gly Phe Tyr Val Trp Lys Asp Arg Lys His Asp Gln 485 490 495 Arg Ser Ser Lys Ala Ile Pro Gln Gly Leu Ala Gln Arg Leu Ile Lys 500 505 510 Pro Leu Ile Glu Gln Thr Glu Lys Gln Leu Ala Asn Asn Ile Val Gln 515 520 525 Asp Ala Asp Leu Ala Asp Ala Gly Val Ile Phe Gly Thr Gly Phe Ala 530 535 540 Pro Phe Thr Gly Gly Pro Ile His Tyr Lys Gln Ser Lys Gly Gly Leu 545 550 555 560 <210> 82 <211> 237 <212> PRT <213> Oligotropha carboxidovorans <400> 82 Met Ser Leu Ser Pro Leu Ala Asn Gly Val Arg Val Leu Thr Leu Asp 1 5 10 15 Arg Pro Ser Lys Ala Asn Ala Leu Asn Ala Glu Val Val Asp Gln Leu 20 25 30 Leu Ala Cys Val Ala Gln Ala Glu Ala Glu Asp Cys Arg Val Leu Ile 35 40 45 Leu Ala Ala Asn Gly Lys Ala Phe Cys Gly Gly Phe Asp Phe Gly Gly 50 55 60 Tyr Glu Ser Met Ser Ala Gly Asp Leu Leu Leu Arg Phe Val Arg Ile 65 70 75 80 Glu Glu Leu Leu Gln Arg Met Arg Gln Ser Ser Phe Val Ser Ile Ala 85 90 95 Leu Val His Gly Ala Ala Met Gly Ala Gly Ala Asp Ile Val Ala Ser 100 105 110 Cys Thr Tyr Arg Ile Gly Thr Asp Ala Ser Arg Phe Arg Phe Pro Gly 115 120 125 Phe Arg Phe Gly Val Ala Leu Gly Thr Arg His Leu Ala Gln Leu Val 130 135 140 Gly Pro Gln Arg Ala Arg Asp Ile Leu Leu Thr Asn Ala Thr Ile Asp 145 150 155 160 Ala Leu Thr Ala Val Asp Ile Gly Leu Leu Thr His Leu Val Asp Ala 165 170 175 Gly Ser Met Arg Gln Lys Ala Asp Glu Ile Ile Ala Gln Ile Gly Ser 180 185 190 Leu Asp Arg Val Ala Arg Asn Arg Ile Leu His Leu Thr Ser Ala Gln 195 200 205 Asn Asn Asp Gly Asp Met Ala Glu Leu Val Lys Ser Val Ser Ala Pro 210 215 220 Gly Leu His Glu Arg Ile Ala Gln Tyr Arg Ala Gly His 225 230 235 <210> 83 <211> 266 <212> PRT <213> Riemerella anatipestifer <400> 83 Met Tyr Lys Leu Ile Asp Val Asp Asn His Phe Glu Gly Lys Leu Gln 1 5 10 15 Ile Ala Tyr Ile Asn Gln Pro Glu Ser Phe Asn Ser Leu Asn Lys Val 20 25 30 Val Leu Glu Glu Leu Leu His Phe Ile Lys Ala Cys Asp Ala Asp Ser 35 40 45 Ser Val Arg Cys Ile Ala Ile Ser Gly Lys Gly Lys Ala Phe Cys Ser 50 55 60 Gly Gln Asn Leu Lys Glu Ala Leu Asp Tyr Lys Ala Glu Ala Asn Glu 65 70 75 80 Glu Arg Phe Ile Gln Arg Ile Val Ile Asp Tyr Tyr Asn Pro Leu Val 85 90 95 Lys Ala Ile Val Tyr Ala Lys Lys Pro Val Ile Ala Leu Val Asn Gly 100 105 110 Pro Ala Val Gly Ala Gly Ala Met Leu Ala Leu Ile Cys Asp Phe Ala 115 120 125 Val Ala Ser Glu Ser Ala Tyr Phe Ser Leu Ala Phe Ser Asn Ile Gly 130 135 140 Leu Val Pro Asp Thr Ala Gly Thr Tyr Tyr Leu Pro Lys Leu Leu Gly 145 150 155 160 Arg Ser Leu Ala Ser Tyr Leu Ala Phe Thr Gly Lys Lys Leu Ser Ala 165 170 175 Lys Glu Ser Leu Glu Arg Gly Leu Val Val Asp Val Phe Ser Asp Ala 180 185 190 Thr Phe Ser Glu Gln Ser Leu Gln Val Leu Glu His Ile Thr His Gln 195 200 205 Pro Thr Val Ala Leu Gly Leu Thr Lys Lys Ala Phe Asn Lys Ser Tyr 210 215 220 Gln Asn Ser Leu Ser Glu Gln Leu Asp Leu Glu Ser Ile Leu Gln Gln 225 230 235 240 Asp Ala Ala Glu Thr Trp Asp Phe Gln Glu Gly Ile Ala Ala Phe Leu 245 250 255 Ala Lys Arg Lys Pro Gln Tyr Lys Gly Lys 260 265 <210> 84 <211> 422 <212> PRT <213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007 <400> 84 Met Ser Glu Thr Ile Asn Leu Asp Glu Met Ser Ala Lys Gln Leu Leu 1 5 10 15 Gly Tyr Tyr Gln Glu Lys Leu Asp Glu Glu Ala Arg Gln Ala Lys Arg 20 25 30 Glu Gly Lys Leu Val Cys Trp Ser Ala Ser Val Ala Pro Pro Glu Phe 35 40 45 Cys Val Ala Met Asp Ile Ala Met Val Tyr Pro Glu Thr His Ala Ala 50 55 60 Gly Ile Gly Ala Arg Lys Gly Ser Leu Asp Leu Leu Glu Val Ala Asp 65 70 75 80 Glu Lys Gly Tyr Ser Leu Asp Ile Cys Ser Tyr Ala Arg Val Asn Leu 85 90 95 Gly Tyr Met Glu Leu Leu Lys Gln Gln Ala Leu Thr Gly Glu Thr Pro 100 105 110 Glu Lys Leu Ala Asn Ser Pro Ala Ala Lys Val Pro Leu Pro Asp Leu 115 120 125 Val Ile Thr Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu 130 135 140 Asn Leu Ala Lys Glu Leu Asn Ile Pro Cys Ile Val Ile Asp Val Pro 145 150 155 160 Phe Asn His Thr Met Pro Ile Thr Lys His Ser Lys Glu Tyr Ile Ala 165 170 175 Asp Gln Phe Lys Tyr Ala Ile Gln Gln Leu Glu Glu Ile Thr Gly Lys 180 185 190 Lys Phe Asp Tyr Asp Lys Phe Leu Glu Val Gln Glu Gln Thr Gln Arg 195 200 205 Ser Val Tyr Gln Trp Asn Arg Leu Ala Ala Leu Ala His Tyr Lys Pro 210 215 220 Ser Pro Leu Asn Gly Phe Asp Leu Phe Asn Phe Met Ala Leu Ile Val 225 230 235 240 Cys Ala Arg Ser Arg Asp Tyr Ala Glu Ile Thr Phe Lys Lys Phe Ala 245 250 255 Asp Glu Leu Glu Glu Asn Leu Lys Asn Glu Val Tyr Ala Phe Lys Gly 260 265 270 Ala Glu Lys Asn Arg Val Thr Trp Glu Gly Ile Ala Val Trp Pro Tyr 275 280 285 Leu Gly His Thr Phe Lys Ser Leu Lys Gly Met Gly Ser Ile Met Thr 290 295 300 Gly Ser Ala Tyr Pro Gly Ile Trp Asn Leu Thr Tyr Thr Pro Gly Asp 305 310 315 320 Met Glu Ser Met Ala Glu Ala Tyr Thr Arg Val Tyr Ile Asn Thr Cys 325 330 335 Leu Gln Asn Lys Ala Asp Val Leu Ser Lys Ile Val Thr Asp Gly Lys 340 345 350 Cys Asp Gly Ile Leu Tyr His Leu Asn Arg Ser Cys Lys Leu Met Ser 355 360 365 Phe Leu Asn Val Glu Thr Ala Glu Leu Val Glu Lys Ala Thr Gly Val 370 375 380 Pro Tyr Val Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ala 385 390 395 400 Pro Ala Gln Phe Asp Thr Arg Val Gln Ala Leu Asn Glu Met Met Glu 405 410 415 Val Asn Asn Glu Thr Lys 420 <210> 85 <211> 277 <212> PRT <213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007 <400> 85 Met Gln Asp Asp Arg Ser Phe Lys Lys Gly Lys Arg Arg Gly Met Tyr 1 5 10 15 Thr Val Gly Val Asp Ile Gly Ser Ser Ser Ser Lys Val Val Ile Leu 20 25 30 Lys Asp Gly Thr Glu Ile Val Ser Gln Ser Ala Ile Gln Ser Gly Ile 35 40 45 Gly Ser Asn Arg Ala Ile Val Ala Leu Glu Asp Asn Leu Lys Lys Ala 50 55 60 Asn Leu Thr Lys Glu Asp Ile Gly Phe Thr Val Val Thr Gly Tyr Gly 65 70 75 80 Arg Phe Thr Phe Glu Gly Ala Asp Lys Gln Ile Ser Glu Ile Ser Cys 85 90 95 His Ala Arg Gly Ile His Phe Leu Leu Pro Asn Val Arg Thr Ile Ile 100 105 110 Asp Ile Gly Gly Gln Asp Ala Lys Ala Ile Ser Leu Asp Glu Lys Gly 115 120 125 His Val Arg Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly Thr Gly 130 135 140 Arg Phe Leu Thr Val Met Ala Arg Val Leu Glu Ile Ser Leu Asp Glu 145 150 155 160 Met Gly Thr Tyr Asp Ala Leu Ser Lys Asn Pro Cys Asn Ile Ser Ser 165 170 175 Thr Cys Ala Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu Ala Lys 180 185 190 Gly Asn Thr Lys Glu Asp Val Ile Ala Gly Val His Asn Ser Val Ala 195 200 205 His Lys Ile Leu Gly Leu Val Tyr Arg Thr Ser Met Glu Glu Lys Phe 210 215 220 Ala Ile Cys Gly Gly Val Ala Gln Asn Thr Gly Ala Leu Arg Ala Ile 225 230 235 240 Arg Glu Ala Leu Lys Lys Glu Val Ile Val Ala Pro Asn Pro Gln Leu 245 250 255 Thr Gly Ala Leu Gly Ala Ala Ile Phe Ala Tyr Asp Glu Leu Lys Lys 260 265 270 Leu Arg Lys Gly Glu 275 <210> 86 <211> 374 <212> PRT <213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007 <400> 86 Met Lys Gly Arg Leu Glu Glu Leu Ile His Ile Phe Glu Asp Val Ala 1 5 10 15 Asn Asn Pro Lys Lys Met Val Ala Glu Tyr Lys Lys Glu Val Gly Lys 20 25 30 Glu Val Ile Gly Val Met Pro Val Tyr Ala Pro Glu Glu Ile Ile His 35 40 45 Ala Ala Gly Cys Leu Pro Ile Gly Leu Trp Gly Gly Lys Lys Glu Val 50 55 60 Ser Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln 65 70 75 80 Thr Val Met Glu Leu Gln Ile Gly Gly Thr Tyr Asp Ile Leu Asp Ala 85 90 95 Val Leu Phe Ser Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys 100 105 110 Trp Lys Gly Lys Ser Pro Val Ile Val Phe Thr His Pro Gln Asn Arg 115 120 125 Val Ile Glu Gly Ala Asn Ala Tyr Leu Val Lys Glu Tyr Gln Ala Val 130 135 140 Lys Glu Lys Leu Glu Gly Ile Leu Gly Arg Thr Ile Pro Met Glu Ala 145 150 155 160 Ile Glu Glu Ser Val Lys Val Tyr Asn Glu Asn Arg Arg Val Met Arg 165 170 175 Glu Phe Val Glu Val Ala Ala Gln Tyr Pro Gln Ile Ile Asp Pro Ile 180 185 190 Val Arg His Asn Val Met Lys Ser Arg Trp Phe Leu Arg Lys Glu Lys 195 200 205 His Thr Glu Tyr Val Lys Glu Leu Ile Ala Glu Leu Lys Lys Glu Thr 210 215 220 Ile Val Pro Trp Asp Gly Lys Lys Val Ile Leu Thr Gly Ile Met Thr 225 230 235 240 Glu Pro Val Glu Leu Leu Gln Ile Phe Lys Asp Glu Lys Leu Ala Ile 245 250 255 Val Ala Asp Asp Leu Ala His Glu Ser Arg Gln Phe Arg Gly Asp Val 260 265 270 Pro Glu Glu Gly Gly Asp Val Leu Tyr Arg Met Ala Lys Trp Trp Gln 275 280 285 Asn Leu Glu Gly Cys Ser Leu Ala Thr Asp Thr Asn Lys Gly Arg Gly 290 295 300 Gln Met Leu Met Asp Met Cys Lys Asp Thr Lys Ala Asp Ala Val Ile 305 310 315 320 Val Cys Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Val 325 330 335 Tyr Tyr Arg Glu Phe Thr Glu Ser Gly Ile Lys Asn Ile Thr Val Glu 340 345 350 Val Asp Leu Glu Val Ser Ser Phe Glu Gln Ile Arg Thr Arg Ile Gln 355 360 365 Thr Phe Lys Asp Ile Leu 370 <210> 87 <211> 422 <212> PRT <213> Desulfosporosinus youngiae DSM 17734 <400> 87 Met Thr Asp Thr Thr Thr Met Ser Ala Lys Glu Leu Leu Gly Phe Tyr 1 5 10 15 Gln Glu Glu Leu Tyr Glu Glu Ala Arg Gln Ala Lys Lys Glu Gly Lys 20 25 30 Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys Val Ala 35 40 45 Met Asp Val Ala Met Ile Tyr Pro Glu Thr His Ala Ala Gly Ile Gly 50 55 60 Ala Arg Lys Gly Ala Leu Asp Val Leu Glu Val Ala Asp Glu Lys Gly 65 70 75 80 Tyr Asn Leu Asp Thr Cys Ser Tyr Ala Arg Val Asn Met Gly Tyr Met 85 90 95 Glu Leu Leu Lys Gln Glu Ala Leu Thr Gly Ile Thr Pro Glu Lys Leu 100 105 110 Glu Lys Ser Pro Ala Ala Arg Ile Pro Leu Pro Asp Phe Val Ile Thr 115 120 125 Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn Leu Ala 130 135 140 Val Glu Leu Asn Ile Pro Cys Ile Ile Ile Asp Val Pro Phe Asn His 145 150 155 160 Thr Met Pro Ile Pro Gln Tyr Ala Lys Asp Tyr Ile Ala Glu Gln Phe 165 170 175 Lys Glu Ala Ile Thr Gln Leu Glu Glu Ile Cys Gly Arg Lys Phe Asp 180 185 190 Tyr Asp Lys Phe Leu Lys Val Gln Glu Gln Thr Gln Arg Ser Val Ala 195 200 205 Gln Trp Asn Arg Ile Ala Ala Leu Ser Gly His Lys Pro Ser Pro Leu 210 215 220 Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys Ala Arg 225 230 235 240 Ser Arg Asp Tyr Ala Glu Ile Thr Phe Lys Lys Phe Ala Asp Glu Leu 245 250 255 Glu Glu Asn Leu Lys Asn Gly Ile Tyr Ala Phe Lys Gly Asn Glu Gln 260 265 270 Lys Arg Val Thr Trp Glu Gly Ile Ala Val Trp Pro His Leu Gly His 275 280 285 Thr Phe Lys Gly Leu Lys Asn Leu Gly Asn Ile Met Thr Gly Ser Ala 290 295 300 Tyr Pro Gly Leu Trp Asn Leu Thr Tyr Thr Pro Gly Asp Met Ser Ser 305 310 315 320 Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu Asp Asn 325 330 335 Lys Val Lys Val Leu Ser Asp Val Ile Ser Gly Gly Lys Cys Asp Gly 340 345 350 Val Ile Tyr His Gln Asn Arg Ser Cys Lys Leu Met Ser Leu Leu Asn 355 360 365 Val Glu Thr Ala Asp Ile Leu Gln Lys Gln Asn His Leu Pro Tyr Val 370 375 380 Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ala Pro Ala Gln 385 390 395 400 Phe Asp Thr Arg Ile Gln Ala Leu Asp Glu Met Met Lys Gln Asn Lys 405 410 415 Glu Gly Val Ser Asn Glu 420 <210> 88 <211> 372 <212> PRT <213> Desulfosporosinus youngiae DSM 17734 <400> 88 Met Ser Arg Ile Glu Thr Ile Ile Ser Glu Leu Thr Ser Ile Ala Asn 1 5 10 15 Asn Pro Arg Gln Ala Met Glu Asp Tyr Lys Lys Glu Thr Gly Lys Gly 20 25 30 Ser Val Gly Val Met Pro Tyr Tyr Ala Pro Glu Glu Ile Ile His Ala 35 40 45 Ala Gly Tyr Leu Pro Val Gly Ile Trp Gly Gly Gln Lys Ser Ile Ser 50 55 60 Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Ser 65 70 75 80 Val Val Glu Met Gln Leu Glu Gly Val Tyr Asp Asp Leu Glu Ala Val 85 90 95 Leu Phe Pro Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys Trp 100 105 110 Lys Gly Thr Ser Pro Val Ile Val Leu Thr His Pro Gln Asn Arg Lys 115 120 125 Leu Glu Ala Ala Asn Lys Phe Leu Ala Glu Glu Tyr Arg Leu Val Arg 130 135 140 Glu Lys Leu Glu Lys Ile Leu Asn Val Lys Ile Thr Asp Glu Ala Leu 145 150 155 160 Asn Gln Ser Ile Glu Ile Tyr Asn Glu Asn Arg Lys Val Met Arg Glu 165 170 175 Phe Thr Glu Ile Ala Ala Asn Tyr Pro Asn Ile Ile Asp Pro Val Lys 180 185 190 Arg His Ala Leu Ile Lys Ala Arg Phe Phe Met Glu Lys Ala Lys His 195 200 205 Thr Ala Leu Val Lys Glu Leu Asn Ala Glu Leu Lys Ala Leu Pro Val 210 215 220 Glu Ala Phe Thr Gly Lys Lys Val Val Leu Thr Gly Ile Met Ala Glu 225 230 235 240 Pro Asn Glu Val Leu Asp Ile Leu Gln Asp Asn Gly Phe Ala Val Val 245 250 255 Ala Asp Asp Leu Ala Gln Glu Ser Arg Leu Phe Arg Asn Asp Val Pro 260 265 270 Ser Gly Thr Asp Pro Leu Tyr Arg Leu Ala Lys Trp Trp Gln Glu Phe 275 280 285 Asp Gly Cys Ser Leu Ala Val Asp Ala Lys Lys Pro Arg Gly Pro Met 290 295 300 Leu Met Asp Met Val Lys Ala Ser Lys Ala Asp Ala Val Val Val Cys 305 310 315 320 Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Tyr 325 330 335 Arg Gln Phe Glu Glu Ala Gly Ile Lys Ser Leu Phe Ile Glu Ile Asp 340 345 350 Leu Glu Pro Thr Ser Phe Glu Gln Thr Lys Thr Arg Val Gln Ser Phe 355 360 365 Arg Glu Met Leu 370 <210> 89 <211> 272 <212> PRT <213> Desulfosporosinus youngiae DSM 17734 <400> 89 Met Phe Thr Met Gly Ile Asp Ile Gly Ser Ser Ser Ser Lys Val Val 1 5 10 15 Ile Leu Glu Asp Gly Val Asn Ile Ile Ala Gly Glu Val Ile Gln Ile 20 25 30 Gly Thr Gly Ser Thr Gly Pro Lys Arg Val Leu Asp Glu Ala Leu Ala 35 40 45 Lys Ala Gly Leu Thr Leu Gln Asp Met Ala Lys Ile Ile Ala Thr Gly 50 55 60 Tyr Gly Arg Ser Ser Val Glu Glu Ala His Lys Gln Ile Ser Glu Ile 65 70 75 80 Ser Cys Gln Ala Lys Gly Val Phe Phe Leu Val Pro Ser Ala Lys Leu 85 90 95 Ile Ile Asp Ile Gly Gly Gln Asp Val Lys Ala Ile Lys Leu Asp Ser 100 105 110 Lys Gly Cys Val Lys Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly 115 120 125 Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Leu Glu Val Asn Leu 130 135 140 Asp Glu Met Ala Glu Tyr Asp Ala Arg Ala Thr Glu Pro Ala Thr Val 145 150 155 160 Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu 165 170 175 Ala Asn Gly Val Ala Lys Glu Asn Ile Ile Ala Gly Val His Gln Ser 180 185 190 Val Ala Ser Lys Ala Cys Gly Leu Ala Tyr Arg Cys Gly Val Glu Glu 195 200 205 Asp Ile Val Met Cys Gly Gly Val Ala Lys Asp Leu Gly Val Val Arg 210 215 220 Ala Ile Ser Lys Glu Leu Lys Lys Pro Val Ile Val Ala Pro Asn Pro 225 230 235 240 Gln Ile Thr Ala Ala Leu Gly Ala Ala Ile Phe Ala Phe Glu Glu Val 245 250 255 Met Glu Thr Val Met Val Ala Phe Glu Glu Val Arg Gly Ala Asn Lys 260 265 270 <210> 90 <211> 422 <212> PRT <213> Peptoniphilus indolicus ATCC 29427 <400> 90 Met Asn Thr Ile Asp Ile Ser Asn Met Lys Ala Lys Glu Met Leu Gly 1 5 10 15 Tyr Phe Gln Asn Lys Leu Asp Glu Glu Ala Arg Glu Ala Lys Lys Asn 20 25 30 Gly Lys Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys 35 40 45 Val Thr Met Asp Ile Ala Leu Val Tyr Pro Glu Thr His Ala Ala Gly 50 55 60 Ile Gly Ala Arg Lys Gly Ser Leu Ala Met Leu Asp Val Ala Asp Arg 65 70 75 80 Lys Gly Tyr Asn Thr Asp Ile Cys Ser Tyr Ala Arg Val Asn Leu Gly 85 90 95 Tyr Met Glu Leu Leu Lys Glu Tyr Ala Lys Thr Gly Val Lys Pro Lys 100 105 110 Glu Leu Glu Glu Ser Pro Ala Ala Asp Val Pro Leu Pro Asp Leu Val 115 120 125 Ile Thr Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn 130 135 140 Leu Ala Ala Glu Leu Asn Ile Pro Cys Ile Val Ile Asp Val Pro Phe 145 150 155 160 Asn His Thr Met Pro Ile Pro Lys Tyr Ser Lys Glu Tyr Ile Ala Asp 165 170 175 Gln Phe Lys Glu Ala Ile Arg Gln Leu Glu Glu Ile Thr Gly Lys Asp 180 185 190 Phe Asp Tyr Asp Lys Phe Leu Glu Val Gln Glu Gln Thr Gln Arg Ser 195 200 205 Val Ala Gln Trp Asn Arg Leu Ala Ala Leu Ser Lys Tyr Glu Pro Ser 210 215 220 Pro Leu Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys 225 230 235 240 Ala Arg Ser Lys Asn Tyr Ala Glu Leu Thr Phe Lys Lys Phe Ala Asp 245 250 255 Glu Leu Glu Glu Asn Met Gln Asn Gly Val Tyr Pro Tyr Lys Ala Gly 260 265 270 Glu Gln Ser Arg Ile Thr Trp Glu Gly Ile Ala Ile Trp Pro Tyr Leu 275 280 285 Gly His Thr Phe Lys Thr Leu Lys Gly Tyr Gly Ser Ile Met Thr Gly 290 295 300 Ser Ala Tyr Pro Gly Leu Trp Asn Leu Glu Tyr Thr Pro Gly Asp Met 305 310 315 320 Leu Ser Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu 325 330 335 Asp Asn Lys Val Asp Val Leu Arg Lys Ile Ile Lys Asn Gly Lys Cys 340 345 350 Asp Gly Val Ala Tyr His Leu Asn Arg Ser Cys Lys Leu Met Ser Leu 355 360 365 Leu Asn Val Glu Thr Ala Glu Ile Leu Asn Lys Glu Asn Asn Leu Pro 370 375 380 Tyr Val Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ser Glu 385 390 395 400 Ala Gln Tyr Asp Asn Arg Ile Gln Thr Leu Thr Glu Met Met Ser Ala 405 410 415 Asn Lys Lys Met Arg Gly 420 <210> 91 <211> 263 <212> PRT <213> Peptoniphilus indolicus ATCC 29427 <400> 91 Met Tyr Thr Met Gly Val Asp Ile Gly Ser Thr Ser Ser Lys Ile Ile 1 5 10 15 Ile Leu Glu Asp Gly Ile Lys Ile Ile Gly Asn Ile Val Val Gln Ser 20 25 30 Gly Thr Gly Thr Ser Gly Pro Thr Ile Ala Thr Ala Lys Ala Lys Ser 35 40 45 Phe Leu Ser Asn Asn Asn Leu Thr Leu Asp Asp Ile Ser Lys Ile Val 50 55 60 Val Thr Gly Tyr Gly Arg Phe Ser Phe Asp Ile Ala Asp Lys Gln Ile 65 70 75 80 Ser Glu Ile Thr Cys His Thr Lys Gly Ile Asn Phe Leu Val Pro Glu 85 90 95 Ala Arg Thr Ile Leu Asp Ile Gly Gly Gln Asp Thr Lys Ala Ile Ser 100 105 110 Val Asn Asp Lys Gly Gln Val Leu Gln Phe Phe Met Asn Asp Lys Cys 115 120 125 Ala Ala Gly Thr Gly Arg Phe Leu Glu Val Met Ala Lys Ile Leu Glu 130 135 140 Ile Pro Leu Glu Lys Met Gly Glu Tyr Asp Arg Leu Ser Thr Asn Pro 145 150 155 160 Val Ala Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile 165 170 175 Ser Gln Leu Ser Lys Gly Ile Ser Lys Glu Asn Ile Leu Ala Gly Val 180 185 190 His Asn Ser Thr Ala Asn Lys Val Cys Gly Leu Leu Tyr Arg Thr Gly 195 200 205 Ile Lys Glu Lys Ile Val Leu Cys Gly Gly Val Ala Gln Asn Gln Gly 210 215 220 Val Val Arg Ala Leu Gln Glu Glu Leu Lys Lys Glu Ile Thr Ile Ala 225 230 235 240 Pro His Pro Gln Met Thr Gly Ala Ile Gly Ala Ala Leu Phe Ala Tyr 245 250 255 Glu Glu Ala Asn Lys Asn Leu 260 <210> 92 <211> 372 <212> PRT <213> Peptoniphilus indolicus ATCC 29427 <400> 92 Met Asn Lys Ile Asn Glu Ile Ile Asn Leu Leu Asp Glu Val Ser Lys 1 5 10 15 Asp Pro Lys Leu Thr Val Lys Lys Tyr Lys Glu Lys Thr Gly Lys Gly 20 25 30 Val Val Gly Val Met Pro Leu Tyr Ala Pro Glu Glu Ile Ile His Ala 35 40 45 Ala Gly Phe Leu Pro Met Gly Leu Trp Gly Ala Gln Lys Glu Val Ser 50 55 60 Lys Ala Arg Ile Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Thr 65 70 75 80 Asn Met Glu Leu Gln Ile Glu Gly Ala Tyr Asp Asp Leu Asp Ala Val 85 90 95 Val Phe Ser Val Pro Cys Asp Thr Leu Lys Cys Met Ser Gln Lys Trp 100 105 110 Lys Gly Lys Ser Pro Val Ile Val Phe Thr His Pro Gln Asn Arg Lys 115 120 125 Leu Glu Ser Ala Asn Lys Phe Leu Val Thr Glu Tyr Glu Ile Leu Lys 130 135 140 Asp Lys Leu Glu Lys Ile Leu Asn Val Lys Ile Ser Asp Glu Ser Ile 145 150 155 160 Thr Asn Ser Ile Glu Ile Tyr Asn Glu Asn Arg Lys Val Met Arg Glu 165 170 175 Phe Ser Asp Leu Ala Gly Gln Tyr Pro Asn Ile Ile Asp Pro Ile Gln 180 185 190 Arg His Ile Val Phe Lys Ser Arg Trp Phe Met Glu Lys Ser Glu His 195 200 205 Thr Lys Leu Val Lys Glu Leu Ile Ser Glu Ile Lys Lys Leu Pro Ile 210 215 220 Glu Glu Trp Asp Gly Tyr Lys Val Ile Ala Thr Gly Ile Met Ile Glu 225 230 235 240 Pro Glu Glu Ile Leu Gln Ile Phe Lys Asp Lys Lys Ile Ala Ile Val 245 250 255 Ala Asp Asp Leu Ala Gln Glu Ser Arg Gln Phe Arg His Asp Val Pro 260 265 270 Glu Gly Asp Gln Pro Leu Leu Arg Leu Ala Lys Trp Trp Gln Asn Leu 275 280 285 Glu Gly Cys Ala Leu Ala Thr Asp Thr Lys Lys Leu Arg Gly Gln Met 290 295 300 Leu Ile Asp Met Ala Lys Lys Tyr Asn Ala Asp Ala Val Leu Ile Cys 305 310 315 320 Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Val Tyr Tyr 325 330 335 Arg Glu Phe Gln Glu Ala Gly Ile Lys Asn Leu Leu Ile Glu Ile Asp 340 345 350 Leu Glu Met Thr Ala Phe Glu Gln Thr Asn Thr Arg Leu Gln Thr Leu 355 360 365 Val Glu Thr Leu 370 <210> 93 <211> 422 <212> PRT <213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10) <400> 93 Met Thr Asp Thr Thr Ala Met Ser Ala Lys Glu Leu Leu Gly Phe Tyr 1 5 10 15 Gln Glu Glu Leu Tyr Glu Glu Ala Arg Arg Ala Lys Lys Glu Gly Lys 20 25 30 Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys Val Ala 35 40 45 Met Asp Val Ala Met Ile Tyr Pro Glu Thr His Ala Ala Gly Ile Gly 50 55 60 Ala Arg Lys Gly Ala Leu Asp Val Leu Glu Val Ala Asp Glu Lys Gly 65 70 75 80 Tyr Asn Val Asp Thr Cys Ser Tyr Ala Arg Val Asn Leu Gly Tyr Met 85 90 95 Glu Leu Leu Lys Gln Glu Ala Leu Thr Gly Ile Thr Pro Glu Lys Leu 100 105 110 Glu Lys Ser Pro Ala Ala Arg Ile Pro Leu Pro Asp Phe Val Ile Thr 115 120 125 Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn Leu Ala 130 135 140 Val Glu Leu Asn Ile Pro Cys Ile Ile Ile Asp Val Pro Phe Asn His 145 150 155 160 Thr Met Pro Ile Pro Gln Tyr Ala Lys Asp Tyr Ile Ala Glu Gln Phe 165 170 175 Lys Glu Ala Ile Thr Gln Leu Glu Glu Ile Cys Gly Lys Lys Phe Asp 180 185 190 Tyr Asp Lys Phe Leu Lys Val Gln Glu Gln Thr Gln Arg Ser Val Ala 195 200 205 Gln Trp Asn Arg Ile Ala Ala Leu Ser Ser His Lys Pro Ser Pro Leu 210 215 220 Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys Ala Arg 225 230 235 240 Ser Lys Asp Tyr Ala Glu Ile Thr Phe Lys Lys Phe Ala Asp Glu Leu 245 250 255 Glu Glu Asn Leu Asn Lys Gly Ile Phe Ala Phe Lys Gly Asn Glu Gln 260 265 270 Lys Arg Val Thr Trp Glu Gly Ile Ala Val Trp Pro His Leu Gly His 275 280 285 Thr Phe Lys Gly Leu Lys Asn Leu Gly Asn Ile Met Thr Gly Ser Ala 290 295 300 Tyr Pro Gly Leu Trp Asn Val Ser Tyr Thr Pro Gly Asp Met Ser Ser 305 310 315 320 Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu Asp Asn 325 330 335 Lys Val Lys Val Leu Ser Asp Val Ile Ser Gly Gly Lys Cys Asp Gly 340 345 350 Val Ile Tyr His Gln Asn Arg Ser Cys Lys Leu Met Ser Phe Leu Asn 355 360 365 Val Glu Thr Ala Asp Ile Leu Gln Lys Glu Asn Gly Leu Pro Tyr Val 370 375 380 Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ser Pro Ala Gln 385 390 395 400 Phe Asp Thr Arg Ile Gln Ala Leu Asp Glu Met Met Lys Gln Asn Lys 405 410 415 Glu Gly Val Ser Asn Glu 420 <210> 94 <211> 372 <212> PRT <213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10) <400> 94 Met Ser Arg Ile Glu Thr Ile Ile Ser Glu Leu Ser Ser Ile Ser Asn 1 5 10 15 Asn Pro Arg Lys Ala Met Glu Asp Tyr Lys Lys Glu Thr Gly Lys Gly 20 25 30 Ser Val Gly Val Met Pro Tyr Tyr Ala Pro Glu Glu Ile Ile His Ala 35 40 45 Ala Gly Phe Leu Pro Val Gly Ile Trp Gly Gly Gln Lys Ser Ile Ser 50 55 60 Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Ser 65 70 75 80 Val Met Glu Met Gln Leu Glu Gly Val Tyr Asp Asp Leu Glu Ala Val 85 90 95 Leu Phe Pro Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys Trp 100 105 110 Lys Gly Thr Ser Pro Val Ile Val Phe Thr His Pro Gln Asn Arg Lys 115 120 125 Leu Glu Ala Ala Asn Lys Phe Leu Ala Glu Glu Tyr Arg Leu Val Arg 130 135 140 Glu Lys Leu Glu Thr Ile Leu Asn Val Lys Ile Thr Asp Glu Ala Leu 145 150 155 160 Asn Gln Ser Ile Glu Thr Tyr Asn Glu Asn Arg Lys Val Met Arg Glu 165 170 175 Phe Thr Asp Leu Ala Ala Asn Tyr Pro Gln Ile Ile Asp Pro Arg Ile 180 185 190 Arg His Ala Ile Ile Lys Ala Arg Phe Phe Met Glu Lys Ser Lys His 195 200 205 Thr Ala Met Val Lys Glu Leu Asn Ser Glu Leu Lys Ser Leu Pro Val 210 215 220 Glu Ala Phe Thr Gly Lys Lys Val Val Leu Thr Gly Ile Met Ala Glu 225 230 235 240 Pro Asn Glu Val Leu Asp Ile Leu Lys Asp Asn Gly Phe Ala Val Val 245 250 255 Ala Asp Asp Leu Ala Gln Glu Ser Arg Leu Phe Arg Asn Asp Val Pro 260 265 270 Ser Gly Thr Asp Pro Leu Tyr Arg Leu Ala Lys Trp Trp Gln Glu Phe 275 280 285 Asp Gly Cys Ser Leu Ala Thr Asp Ala Lys Lys Ser Arg Gly Pro Met 290 295 300 Leu Met Glu Met Val Lys Gly Ser Lys Ala Asp Ala Val Val Val Cys 305 310 315 320 Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Tyr 325 330 335 Arg Gln Phe Glu Glu Ala Gly Ile Lys Ser Leu Phe Ile Glu Ile Asp 340 345 350 Leu Glu Thr Thr Ser Phe Glu Gln Thr Lys Thr Arg Val Gln Ser Phe 355 360 365 Ser Glu Met Leu 370 <210> 95 <211> 261 <212> PRT <213> Desulfosporosinus meridiei (strain ATCC BAA-275 / DSM 13257 / NCIMB 13706 / S10) <400> 95 Met Phe Thr Met Gly Ile Asp Ile Gly Ser Ser Ser Ser Lys Val Val 1 5 10 15 Ile Leu Glu Asp Gly Val Asn Ile Ile Ala Gly Glu Val Ile Gln Ile 20 25 30 Gly Thr Gly Ser Thr Gly Pro Lys Arg Val Leu Asn Glu Ala Leu Ser 35 40 45 Lys Ala Gly Leu Lys Leu Glu Asp Met Ala Lys Ile Ile Ala Thr Gly 50 55 60 Tyr Gly Arg Ser Ser Val Glu Glu Ala His Lys Gln Ile Ser Glu Ile 65 70 75 80 Ser Cys Gln Ala Lys Gly Val Phe Phe Leu Val Pro Ser Ala Lys Leu 85 90 95 Ile Ile Asp Ile Gly Gly Gln Asp Val Lys Ala Ile Arg Leu Asp Ser 100 105 110 Lys Gly Gly Val Lys Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly 115 120 125 Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Leu Glu Val Asn Leu 130 135 140 Asp Glu Met Ala Glu Tyr Asp Ala Arg Ala Thr Glu Pro Ala Thr Val 145 150 155 160 Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu 165 170 175 Ser Asn Gly Val Ala Lys Glu Asn Ile Ile Ala Gly Val His Gln Ser 180 185 190 Val Ala Ser Lys Ala Cys Gly Leu Ala Tyr Arg Cys Gly Val Glu Glu 195 200 205 Asp Ile Val Met Cys Gly Gly Val Ala Lys Asp Leu Gly Val Val Arg 210 215 220 Ala Ile Ser Lys Glu Leu Lys Lys Pro Val Ile Val Ala Pro Asn Pro 225 230 235 240 Gln Ile Thr Ala Ala Leu Gly Ala Ala Ile Phe Ala Phe Glu Glu Val 245 250 255 Arg Gly Ala Asn Lys 260 <210> 96 <211> 477 <212> PRT <213> Acidaminococcus fermentans <400> 96 Met Pro Lys Thr Val Ser Pro Gly Val Gln Ala Leu Arg Asp Val Val 1 5 10 15 Glu Lys Val Tyr Arg Glu Leu Arg Glu Ala Lys Glu Arg Gly Glu Lys 20 25 30 Val Gly Trp Ser Ser Ser Lys Phe Pro Cys Glu Leu Ala Glu Ser Phe 35 40 45 Gly Leu His Val Gly Tyr Pro Glu Asn Gln Ala Ala Gly Ile Ala Ala 50 55 60 Asn Arg Asp Gly Glu Val Met Cys Gln Ala Ala Glu Asp Ile Gly Tyr 65 70 75 80 Asp Asn Asp Ile Cys Gly Tyr Ala Arg Ile Ser Leu Ala Tyr Ala Ala 85 90 95 Gly Phe Arg Gly Ala Asn Lys Met Asp Lys Asp Gly Asn Tyr Val Ile 100 105 110 Asn Pro His Ser Gly Lys Gln Met Lys Asp Ala Asn Gly Lys Lys Val 115 120 125 Phe Asp Ala Asp Gly Lys Pro Val Ile Asp Pro Lys Thr Leu Lys Pro 130 135 140 Phe Ala Thr Thr Asp Asn Ile Tyr Glu Ile Ala Ala Leu Pro Glu Gly 145 150 155 160 Glu Glu Lys Thr Arg Arg Gln Asn Ala Leu His Lys Tyr Arg Gln Met 165 170 175 Thr Met Pro Met Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn 180 185 190 Cys Met Thr Lys Trp Tyr Glu Asp Ile Ala Arg Arg His Asn Ile Pro 195 200 205 Leu Ile Met Ile Asp Val Pro Tyr Asn Glu Phe Asp His Val Asn Glu 210 215 220 Ala Asn Val Lys Tyr Ile Arg Ser Gln Leu Asp Thr Ala Ile Arg Gln 225 230 235 240 Met Glu Glu Ile Thr Gly Lys Lys Phe Asp Glu Asp Lys Phe Glu Gln 245 250 255 Cys Cys Gln Asn Ala Asn Arg Thr Ala Lys Ala Trp Leu Lys Val Cys 260 265 270 Asp Tyr Leu Gln Tyr Lys Pro Ala Pro Phe Asn Gly Phe Asp Leu Phe 275 280 285 Asn His Met Ala Asp Val Val Thr Ala Arg Gly Arg Val Glu Ala Ala 290 295 300 Glu Ala Phe Glu Leu Leu Ala Lys Glu Leu Glu Gln His Val Lys Glu 305 310 315 320 Gly Thr Thr Thr Ala Pro Phe Lys Glu Gln His Arg Ile Met Phe Glu 325 330 335 Gly Ile Pro Cys Trp Pro Lys Leu Pro Asn Leu Phe Lys Pro Leu Lys 340 345 350 Ala Asn Gly Leu Asn Ile Thr Gly Val Val Tyr Ala Pro Ala Phe Gly 355 360 365 Phe Val Tyr Asn Asn Leu Asp Glu Leu Val Lys Ala Tyr Cys Lys Ala 370 375 380 Pro Asn Ser Val Ser Ile Glu Gln Gly Val Ala Trp Arg Glu Gly Leu 385 390 395 400 Ile Arg Asp Asn Lys Val Asp Gly Val Leu Val His Tyr Asn Arg Ser 405 410 415 Cys Lys Pro Trp Ser Gly Tyr Met Pro Glu Met Gln Arg Arg Phe Thr 420 425 430 Lys Asp Met Gly Ile Pro Thr Ala Gly Phe Asp Gly Asp Gln Ala Asp 435 440 445 Pro Arg Asn Phe Asn Ala Ala Gln Tyr Glu Thr Arg Val Gln Gly Leu 450 455 460 Val Glu Ala Met Glu Ala Asn Asp Glu Lys Lys Gly Lys 465 470 475 <210> 97 <211> 379 <212> PRT <213> Acidaminococcus fermentans <400> 97 Met Ala Ile Ser Ala Leu Ile Glu Glu Phe Gln Lys Val Ser Ala Ser 1 5 10 15 Pro Lys Thr Met Leu Ala Lys Tyr Lys Ala Gln Gly Lys Lys Ala Ile 20 25 30 Gly Cys Leu Pro Tyr Tyr Val Pro Glu Glu Leu Val Tyr Ala Ala Gly 35 40 45 Met Val Pro Met Gly Val Trp Gly Cys Asn Gly Lys Gln Glu Val Arg 50 55 60 Ser Lys Glu Tyr Cys Ala Ser Phe Tyr Cys Thr Ile Ala Gln Gln Ser 65 70 75 80 Leu Glu Met Leu Leu Asp Gly Thr Leu Asp Gly Leu Asp Gly Ile Ile 85 90 95 Thr Pro Val Leu Cys Asp Thr Leu Arg Pro Met Ser Gln Asn Phe Lys 100 105 110 Val Ala Met Lys Asp Lys Met Pro Val Ile Phe Leu Ala His Pro Gln 115 120 125 Val Arg Gln Asn Ala Ala Gly Lys Gln Phe Thr Tyr Asp Ala Tyr Ser 130 135 140 Glu Val Lys Gly His Leu Glu Glu Ile Cys Gly His Glu Ile Thr Asn 145 150 155 160 Asp Ala Ile Leu Asp Ala Ile Lys Val Tyr Asn Lys Ser Arg Ala Ala 165 170 175 Arg Arg Glu Phe Cys Lys Leu Ala Asn Glu His Pro Asp Leu Ile Pro 180 185 190 Ala Ser Val Arg Ala Thr Val Leu Arg Ala Ala Tyr Phe Met Leu Lys 195 200 205 Asp Glu Tyr Thr Glu Lys Leu Glu Glu Leu Asn Lys Glu Leu Ala Ala 210 215 220 Ala Pro Ala Gly Lys Phe Asp Gly His Lys Val Val Val Ser Gly Ile 225 230 235 240 Ile Tyr Asn Met Pro Gly Ile Leu Lys Ala Met Asp Asp Asn Lys Leu 245 250 255 Ala Ile Ala Ala Asp Asp Cys Ala Tyr Glu Ser Arg Ser Phe Ala Val 260 265 270 Asp Ala Pro Glu Asp Leu Asp Asn Gly Leu Gln Ala Leu Ala Val Gln 275 280 285 Phe Ser Lys Gln Lys Asn Asp Val Leu Leu Tyr Asp Pro Glu Phe Ala 290 295 300 Lys Asn Thr Arg Ser Glu His Val Cys Asn Leu Val Lys Glu Ser Gly 305 310 315 320 Ala Glu Gly Leu Ile Val Phe Met Met Gln Phe Cys Asp Pro Glu Glu 325 330 335 Met Glu Tyr Pro Asp Leu Lys Lys Ala Leu Asp Ala His His Ile Pro 340 345 350 His Val Lys Ile Gly Val Asp Gln Met Thr Arg Asp Phe Gly Gln Ala 355 360 365 Gln Thr Ala Leu Glu Ala Phe Ala Glu Ser Leu 370 375 <210> 98 <211> 260 <212> PRT <213> Acidaminococcus fermentans <400> 98 Met Ser Ile Tyr Thr Leu Gly Ile Asp Val Gly Ser Thr Ala Ser Lys 1 5 10 15 Cys Ile Ile Leu Lys Asp Gly Lys Glu Ile Val Ala Lys Ser Leu Val 20 25 30 Ala Val Gly Thr Gly Thr Ser Gly Pro Ala Arg Ser Ile Ser Glu Val 35 40 45 Leu Glu Asn Ala His Met Lys Lys Glu Asp Met Ala Phe Thr Leu Ala 50 55 60 Thr Gly Tyr Gly Arg Asn Ser Leu Glu Gly Ile Ala Asp Lys Gln Met 65 70 75 80 Ser Glu Leu Ser Cys His Ala Met Gly Ala Ser Phe Ile Trp Pro Asn 85 90 95 Val His Thr Val Ile Asp Ile Gly Gly Gln Asp Val Lys Val Ile His 100 105 110 Val Glu Asn Gly Thr Met Thr Asn Phe Gln Met Asn Asp Lys Cys Ala 115 120 125 Ala Gly Thr Gly Arg Phe Leu Asp Val Met Ala Asn Ile Leu Glu Val 130 135 140 Lys Val Ser Asp Leu Ala Glu Leu Gly Ala Lys Ser Thr Lys Arg Val 145 150 155 160 Ala Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser 165 170 175 Gln Leu Ser Lys Gly Thr Asp Lys Ile Asp Ile Ile Ala Gly Ile His 180 185 190 Arg Ser Val Ala Ser Arg Val Ile Gly Leu Ala Asn Arg Val Gly Ile 195 200 205 Val Lys Asp Val Val Met Thr Gly Gly Val Ala Gln Asn Tyr Gly Val 210 215 220 Arg Gly Ala Leu Glu Glu Gly Leu Gly Val Glu Ile Lys Thr Ser Pro 225 230 235 240 Leu Ala Gln Tyr Asn Gly Ala Leu Gly Ala Ala Leu Tyr Ala Tyr Lys 245 250 255 Lys Ala Ala Lys 260 <210> 99 <211> 336 <212> PRT <213> Carboxydothermus hydrogenoformans <400> 99 Met Lys Leu Asn Tyr Phe Cys Ser Tyr Trp Pro Val Glu Ile Ser Glu 1 5 10 15 Gly Ala Gly Ile Ser Thr Val Arg Tyr Phe Pro Ser Asp Glu Ser Lys 20 25 30 Ala Pro Val Arg Leu Pro Ala Tyr Cys Cys Ser Tyr Ala Arg Gly Ser 35 40 45 Leu Ala Glu Ile Glu Glu Glu Gly Asp Gly Asp Phe Trp Gly Phe Ala 50 55 60 His Ser Cys Asp Thr Met Gln Ser Leu Tyr Gly Ile Thr Lys Ser Leu 65 70 75 80 Leu Gly Asp Asp Arg Val Phe Leu Phe Val Pro Pro Val Asp Leu Thr 85 90 95 Thr Ala Phe Ala Arg Glu Tyr Tyr Arg Glu Ala Leu Ile Tyr Leu Trp 100 105 110 Arg Glu Leu Ser Gln Lys Ser Gly Val Asn Gly Glu Glu Lys Leu Lys 115 120 125 Leu Thr Trp Glu Lys Leu Lys Glu Leu Arg Asn Lys Val Lys Ser Leu 130 135 140 Glu Asn Leu Thr Ser Ile Ile Pro Ser Ser Glu Ile Phe Glu Leu Leu 145 150 155 160 Lys Lys Leu Gln Thr Leu Pro Leu Asp Glu Ala Leu Asp Tyr Leu Glu 165 170 175 Ala Lys Lys Ala Glu Phe Thr Ser Leu Ser Val Ala Gln Lys Ala Ile 180 185 190 Gly Ile Ile Leu Thr Gly Ala Val Val Thr Asn Ser Lys Leu Tyr Leu 195 200 205 Ala Leu Glu Gln Gln Gly Phe Arg Val Val Tyr Asp Asp Thr Cys Thr 210 215 220 Gly Phe Arg His Phe Ala Gly Glu Ile Glu Asp Lys Asp Asp Ile Leu 225 230 235 240 Glu Ala Ile Val Ser Tyr Tyr Leu Ser Lys Pro Pro Cys Pro Cys Arg 245 250 255 His Lys Gly Val Trp Ala Arg Ala Glu Tyr Leu Lys Asn Leu Tyr His 260 265 270 Asn Lys Asn Ala Arg Ala Ile Val Leu Leu Gln Asn Lys Phe Cys Asp 275 280 285 Pro Phe Ala Trp Asp Val Pro Tyr Leu Val Asp Tyr Phe Lys Lys Gln 290 295 300 Gly Val Pro Val Leu Val Leu Glu Val Glu Gly Gly Glu Ile Gly Glu 305 310 315 320 Gln Asn Lys Thr Arg Leu Gln Ala Phe Arg Glu Ser Val Gly Gly Val 325 330 335 <210> 100 <211> 404 <212> PRT <213> Carboxydothermus hydrogenoformans <400> 100 Met Ala Lys Lys Ile Phe Lys Pro Leu Lys Ala Ser Glu Lys Ile Asn 1 5 10 15 Lys Ile Leu Lys Asn His Tyr Leu Lys Ala Lys Tyr Leu Pro Thr Leu 20 25 30 Gly Lys Phe Phe Gly Tyr Lys Thr Ala Trp Ile Thr Ser Gly Ala Pro 35 40 45 Val Glu Leu Leu Arg Ala Phe Gly Ile Glu Pro Val Tyr Pro Glu Asn 50 55 60 Tyr Gly Ala Ile Cys Gly Ala Arg Lys Val Ser Pro Ser Leu Cys Gln 65 70 75 80 Val Ala Glu Asn Arg Gly Tyr Ser Leu Asp Leu Cys Ser Tyr Ala Lys 85 90 95 Ser Asn Leu Gly Ser Ile Trp Asn Pro Lys Glu Ser Pro Phe Asn Gly 100 105 110 Leu Pro Arg Pro Asp Leu Leu Val Val Cys Asn Asn Ile Cys Gly Thr 115 120 125 Val Leu Lys Trp Tyr Glu Thr Leu Ser Arg Glu Phe Asn Ile Pro Leu 130 135 140 Phe Ile Ile Asp Thr Pro Phe Ile Thr Gly Glu Pro Gln Pro Trp Gln 145 150 155 160 Ile Gln Tyr Val Ala Lys Gln Ile Glu Lys Leu Ala Ile Glu Leu Glu 165 170 175 Lys Phe Phe Arg Lys Lys Leu Asp Leu Asn Arg Leu Glu Lys Val Ile 180 185 190 Leu Leu Ala Asn Glu Thr Val Asp Leu Trp Lys Gly Ile Arg Asn Phe 195 200 205 Ala Lys Asn Lys Pro Ser Pro Val Asn Val Thr Asp Leu Phe Ile Asn 210 215 220 Leu Gly Pro Met Val Val Leu Arg Gly Thr Glu Val Ala Arg Asp Phe 225 230 235 240 Tyr Glu Glu Val Tyr Arg Glu Val Glu Glu Arg Tyr Lys Ala Gly Val 245 250 255 Pro Ala Val Glu Gly Glu Lys Tyr Arg Leu Val Trp Asp Asn Ile Pro 260 265 270 Ile Trp Tyr Gly Leu Tyr Arg Phe Tyr Gly Tyr Phe Ala Glu Arg Gly 275 280 285 Ala Val Phe Val Thr Asp Ser Tyr Thr Gly Gly Trp Ala Val Asn Ile 290 295 300 Lys Lys Gly Pro Pro Phe Tyr Ala Leu Ala Glu Thr Tyr Ala Gly Val 305 310 315 320 Phe Leu Asn Arg Asp Leu Glu Phe Arg Lys Asn Gln Leu Gln Ser Phe 325 330 335 Ile Glu Glu Phe Ser Ala Asp Gly Phe Val Met His Ser Asn Arg Ser 340 345 350 Cys Lys Ala Tyr Ser Phe Val Gln Glu Glu Ile Arg Arg Gln Ile Met 355 360 365 Arg Ser Leu Gly Val Pro Gly Leu Ile Val Asp Ala Asp Met Thr Asp 370 375 380 Ser Arg Leu Tyr Ser Glu Glu Thr Val Leu Asn Arg Val Gln Ala Phe 385 390 395 400 Leu Glu Ser Leu <210> 101 <211> 254 <212> PRT <213> Carboxydothermus hydrogenoformans <400> 101 Met Tyr Leu Gly Val Asp Ile Gly Ser Leu Thr Thr Lys Val Val Leu 1 5 10 15 Ile Asp Arg Gly Lys Asn Leu Ile Ala Tyr Arg Tyr Ser Lys Thr Gly 20 25 30 Pro Ala Gly Lys Glu Thr Ala Glu Arg Leu Ile Gln Glu Val Leu Ile 35 40 45 Lys Ala Asn Ile Ser Arg Asp Asp Ile Gln Gly Ile Val Ala Thr Gly 50 55 60 Tyr Gly Arg Val Leu Phe Ser Gly Lys Glu Phe Ser Glu Ile Thr Cys 65 70 75 80 Gln Ala Arg Gly Ile Gly His Leu Tyr Pro Glu Ala Lys Thr Ile Ile 85 90 95 Asp Ile Gly Gly Gln Asp Ser Lys Val Ile Ser Leu Gly Lys Asn Gly 100 105 110 Lys Val Leu Asp Phe Ala Met Asn Asp Lys Cys Ala Ala Gly Thr Gly 115 120 125 Arg Phe Leu Glu Val Met Ser Gln Ala Leu Glu Val Arg Leu Glu Glu 130 135 140 Ile Gly Glu Leu Ala Glu Lys Ser Gln Glu Ala Ala Lys Ile Ser Ser 145 150 155 160 Val Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Asn Leu Ser Arg 165 170 175 Gly Gln Ser Arg Glu Ala Val Ala Arg Gly Ile Cys Glu Ala Val Ala 180 185 190 Ala Arg Thr Ala Ile Leu Ala Gln Lys Val Gly Val Val Glu Pro Val 195 200 205 Val Phe Thr Gly Gly Val Ala Lys Asn Thr Gly Val Val Ala Ala Leu 210 215 220 Glu Arg Lys Leu Gly Val Lys Leu Leu Ile Pro Glu Asp Ser Thr Ile 225 230 235 240 Thr Ala Ala Leu Gly Ala Ala Leu Leu Ala Ala Glu Asn Ser 245 250 <210> 102 <211> 261 <212> PRT <213> Oscillibacter valericigenes <400> 102 Met Asn Asn Ile Tyr Thr Met Gly Ile Asp Val Gly Ser Thr Ala Ser 1 5 10 15 Lys Cys Leu Ile Leu Lys Asp Gly Ser Glu Ile Val Ala Lys Ser Leu 20 25 30 Val Asp Val Gly Ala Gly Thr Ser Gly Pro Thr Arg Ala Ile Ala Glu 35 40 45 Val Leu Glu Ala Ala Gly Met Lys Lys Glu Asp Met Ala Phe Ile Leu 50 55 60 Ala Thr Gly Tyr Gly Arg Asn Ser Leu Asp Asp Ile Ala Asp His Gln 65 70 75 80 Met Ser Glu Leu Ser Cys His Ala Lys Gly Ala Phe Phe Leu Phe Pro 85 90 95 Asp Val His Thr Val Ile Asp Ile Gly Gly Gln Asp Val Lys Ile Leu 100 105 110 Glu Ile Glu Asn Gly Val Met Val Asn Phe Ala Met Asn Asp Lys Cys 115 120 125 Ala Ala Gly Thr Gly Arg Phe Leu Asp Val Met Ala Arg Val Leu Glu 130 135 140 Val Lys Val Glu Asp Leu Ala Asp Leu Gly Ala Gln Ser Thr Lys Asn 145 150 155 160 Val Glu Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile 165 170 175 Ser Gln Leu Ala Lys Gly Ser Asp Lys Arg Asp Ile Ile His Gly Ile 180 185 190 His Lys Ser Val Ala Ser Arg Val Val Gly Leu Ala Asn Arg Ile Gly 195 200 205 Val Arg Asp Ala Val Val Met Thr Gly Gly Val Ala Gln Asn Gly Gly 210 215 220 Val Val Ser Ala Leu Gln Glu Ala Leu Gly His Pro Ile His Thr Ser 225 230 235 240 Pro Leu Thr Gln Tyr Asn Gly Ala Leu Gly Ala Ala Leu Phe Ala Trp 245 250 255 Gln Lys Ala Thr Lys 260 <210> 103 <211> 427 <212> PRT <213> Oscillibacter valericigenes <400> 103 Met Ala Glu Asn Glu Lys Ala Thr Ala Ala Ala Pro Glu Ala Ala Pro 1 5 10 15 Val Lys Lys Ala Pro Lys Pro Val Ser Pro Gly Thr Gln Ala Leu Arg 20 25 30 Asp Val Val Thr Lys Val Tyr Ala Ala Ala Trp Asp Ala Lys Lys Ala 35 40 45 Gly Arg Pro Val Gly Trp Ser Ser Ser Lys Phe Pro Cys Glu Ile Ala 50 55 60 Glu Ala Leu Gly Leu Ala Val Val Tyr Pro Glu Asn Gln Ala Ala Gly 65 70 75 80 Ile Gly Ala Gln His Asp Gly Gln Arg Met Cys Glu Ser Ala Glu Ser 85 90 95 Leu Gly Phe Asp Pro Asp Ile Cys Gly Tyr Ala Arg Ile Ser Leu Ala 100 105 110 Tyr Ser Ala Gly Val Glu Thr Thr Asn Glu Ser Arg Arg Val Pro Met 115 120 125 Pro Asp Phe Val Leu Cys Cys Asn Asn Ile Cys Asn Cys Met Thr Lys 130 135 140 Trp Tyr Glu Asn Ile Ala Arg Met His Asn Ile Pro Leu Ile Met Ile 145 150 155 160 Asp Val Pro Tyr Asn Asn Glu Val Thr Val Ser Asp Ser Gln Val Ala 165 170 175 Tyr Ile Arg Gly Gln Phe Asp Asp Ala Ile Lys Gln Met Glu Lys Ile 180 185 190 Ala Gly Val Lys Phe Asp Glu Lys Lys Phe Glu Gln Ala Cys Ala Asn 195 200 205 Ala Asn Arg Thr Ala Lys Ala Trp Leu Thr Val Cys Asp Tyr Leu Gln 210 215 220 Tyr Lys Pro Ala Pro Met Ser Gly Phe Asp Leu Phe Asn His Met Ala 225 230 235 240 Asp Val Val Thr Ala Arg Gly Lys Val Glu Thr Ala Glu Ala Phe Glu 245 250 255 Leu Leu Ala Ser Glu Leu Glu Gln His Val Lys Asn Gly Thr Ser Thr 260 265 270 Ala Pro Phe Pro Glu Gln Tyr Arg Val Met Phe Glu Gly Ile Pro Cys 275 280 285 Trp Pro Asn Leu Arg Thr Leu Phe Lys Pro Leu Lys Ala Asn Gly Val 290 295 300 Asn Val Thr Ala Val Val Tyr Ala Pro Ala Phe Gly Phe Val Tyr Asn 305 310 315 320 Gly Leu Asp Glu Met Ala Arg Ala Tyr Cys Lys Ala Pro Asn Ser Val 325 330 335 Cys Ile Glu Gln Gly Val Asp Trp Arg Glu Gly Ile Cys Arg Glu Asn 340 345 350 Lys Val Asp Gly Val Leu Val His Tyr Asn Arg Ser Cys Lys Pro Trp 355 360 365 Ser Gly Tyr Met Ala Glu Met Gln Arg Arg Phe Thr Lys Asp Leu Gly 370 375 380 Val Pro Cys Ala Gly Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe 385 390 395 400 Asn Glu Ala Gln Tyr Glu Thr Arg Val Gln Gly Leu Val Glu Ala Met 405 410 415 Glu Glu Asn Lys Lys Gln Lys Glu Ala Arg Ala 420 425 <210> 104 <211> 380 <212> PRT <213> Oscillibacter valericigenes <400> 104 Met Ser Ile Glu Thr Ile Val Lys Glu Phe Ala Asp Val Ala Ala Asp 1 5 10 15 Pro Lys Ala Gln Leu Lys Lys Tyr Lys Ala Glu Gly Lys Lys Cys Ile 20 25 30 Gly Val Met Pro Tyr Tyr Ala Pro Glu Glu Leu Val Ala Ala Ala Gly 35 40 45 Met Val Pro Phe Gly Met Trp Gly Ser Asn Asp Lys Thr Ile Ser Arg 50 55 60 Ala Lys Glu Tyr Cys Ala Thr Phe Tyr Cys Thr Ile Ala Gln Leu Asp 65 70 75 80 Leu Glu Met Leu Leu Asp Gly Thr Met Asp Leu Leu Asp Gly Val Ile 85 90 95 Thr Pro Thr Ile Cys Asp Thr Leu Arg Pro Met Ser Gln Asn Ile Arg 100 105 110 Val Ala Met Gly Glu Lys Leu Pro Cys Ile Phe Leu Ala His Pro Gln 115 120 125 Asn Arg Lys Pro Ala Tyr Gly Lys Lys Phe Cys Leu Asp Gln Tyr Thr 130 135 140 His Ile Lys Thr Glu Leu Glu Lys Ile Ala Gly Ala Pro Ile Thr Asp 145 150 155 160 Ala Ala Leu Ser Glu Thr Ile Lys Val Tyr Asn Lys Ser Arg Ala Ala 165 170 175 Arg Arg Glu Phe Val Lys Leu Val Ser Asp His Cys Asp Val Ile Thr 180 185 190 Pro Thr Lys Arg Ser Ala Val Leu Lys Ala Ala Trp Phe Met Pro Lys 195 200 205 Ala Glu Tyr Thr Glu Lys Leu Lys Ala Leu Asn Ala Glu Leu Lys Ala 210 215 220 Leu Pro Val Cys Asp Trp Lys Gly Thr Lys Val Val Thr Ser Gly Ile 225 230 235 240 Ile Cys Asp Asn Pro Lys Leu Leu Glu Ile Phe Glu Glu Asn Lys Ile 245 250 255 Ala Ile Ala Ala Asp Asp Val Ala His Glu Ser Arg Ser Phe Arg Val 260 265 270 Asp Ala Pro Glu Thr Gly Asp Pro Met Glu Ala Leu Ala Gln Gln Phe 275 280 285 Ala Asn Gln Asp Tyr Asp Val Leu Leu Tyr Asp Glu His Ser Ser Glu 290 295 300 Asn Arg Arg Gly Glu Phe Val Ala Lys Leu Val Lys Asp Ser Gly Ala 305 310 315 320 Lys Gly Leu Val Leu Phe Met Gln Gln Phe Cys Asp Pro Glu Glu Met 325 330 335 Glu Tyr Pro Ser Leu Lys Lys Ala Leu Asp Glu Ala Lys Ile Pro His 340 345 350 Ile Lys Leu Gly Val Asp Gln Gln Met Arg Asp Phe Gly Gln Ala Arg 355 360 365 Thr Ala Ile Gln Ala Phe Ala Asp Val Ile Ser Leu 370 375 380 <210> 105 <211> 422 <212> PRT <213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) <400> 105 Met Thr Asp Thr Ala Asn Met Ser Ala Lys Glu Leu Leu Gly Phe Tyr 1 5 10 15 Gln Glu Glu Leu Tyr Glu Glu Ala Arg Gln Ala Lys Lys Glu Gly Lys 20 25 30 Leu Val Cys Trp Ser Ala Ser Val Ala Pro Ser Glu Phe Cys Val Ala 35 40 45 Met Asp Val Ala Met Ile Tyr Pro Glu Thr His Ala Ala Gly Ile Gly 50 55 60 Ala Arg Lys Gly Ala Leu Asp Met Leu Glu Val Ala Asp Glu Lys Gly 65 70 75 80 Tyr Asn Leu Asp Thr Cys Ser Tyr Ala Arg Val Asn Leu Gly Tyr Met 85 90 95 Glu Leu Leu Lys Gln Glu Ala Leu Thr Gly Ile Thr Pro Glu Lys Leu 100 105 110 Glu Lys Ser Pro Ala Ala Arg Val Pro Leu Pro Asp Phe Val Ile Thr 115 120 125 Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp Tyr Glu Asn Leu Ala 130 135 140 Val Glu Leu Asn Ile Pro Cys Ile Val Ile Asp Val Pro Phe Asn His 145 150 155 160 Thr Met Pro Ile Pro Gln Tyr Ala Lys Asp Tyr Ile Ala Glu Gln Phe 165 170 175 Lys Glu Ala Ile Ala Gln Leu Glu Glu Ile Cys Gly Lys Lys Phe Asp 180 185 190 Tyr Asp Lys Phe Leu Gln Val Gln Glu Gln Thr Gln Arg Ser Val Ala 195 200 205 Gln Trp Asn Arg Ile Ala Ser Leu Ser Gly His Lys Pro Ser Pro Leu 210 215 220 Asn Gly Phe Asp Leu Phe Asn Tyr Met Ala Leu Ile Val Cys Ala Arg 225 230 235 240 Ser Arg Asp Cys Ala Glu Ile Thr Phe Lys Lys Phe Ala Asp Glu Leu 245 250 255 Glu Asp Asn Leu Ser Lys Gly Ile Tyr Ala Phe Lys Gly Asn Glu Gln 260 265 270 Lys Arg Ile Thr Trp Glu Gly Ile Ala Val Trp Pro His Leu Gly His 275 280 285 Thr Phe Lys Gly Leu Lys Asn Leu Gly Asn Ile Met Thr Gly Ser Ala 290 295 300 Tyr Pro Gly Leu Trp Asn Leu Ser Tyr Thr Pro Gly Asp Met Ser Ser 305 310 315 320 Met Ala Glu Ala Tyr Thr Arg Ile Tyr Ile Asn Thr Cys Leu Asp Asn 325 330 335 Lys Val Lys Val Leu Ser Asp Ile Ile Ser Gly Gly Lys Cys Asp Gly 340 345 350 Val Ile Tyr His Gln Asn Arg Ser Cys Lys Leu Met Ser Phe Leu Asn 355 360 365 Val Glu Thr Ala Asp Ile Leu Gln Gln Gln Asn His Leu Pro Tyr Val 370 375 380 Ser Phe Asp Gly Asp Gln Thr Asp Pro Arg Asn Phe Ala Pro Ala Gln 385 390 395 400 Phe Asp Thr Arg Ile Gln Ala Leu Asp Glu Met Met Lys Gln Asn Lys 405 410 415 Glu Gly Val Ser His Glu 420 <210> 106 <211> 372 <212> PRT <213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) <400> 106 Met Ser Arg Ile Glu Ala Ile Ile Ser Glu Leu Ser Ser Ile Ala Asn 1 5 10 15 Asn Pro Arg Lys Ala Met Glu Asp Tyr Lys Lys Glu Thr Gly Lys Gly 20 25 30 Ser Val Gly Ile Met Pro Tyr Tyr Ala Pro Glu Glu Ile Val His Ala 35 40 45 Ala Gly Tyr Leu Pro Val Gly Ile Trp Gly Gly Gln Lys Ser Ile Ser 50 55 60 Lys Ala Arg Ala Tyr Leu Pro Pro Phe Ala Cys Ser Ile Met Gln Ser 65 70 75 80 Val Val Glu Met Gln Leu Glu Gly Val Tyr Asn Asp Leu Ala Ala Val 85 90 95 Leu Phe Pro Val Pro Cys Asp Thr Leu Lys Cys Leu Ser Gln Lys Trp 100 105 110 Lys Gly Thr Ser Pro Val Ile Val Met Thr His Pro Gln Asn Arg Lys 115 120 125 Leu Glu Ala Ala Asn Lys Phe Leu Ala Glu Glu Tyr Arg Leu Val Arg 130 135 140 Glu Lys Leu Glu Lys Ile Leu Asn Val Gln Ile Thr Asp Glu Ala Leu 145 150 155 160 Asn His Ser Ile Asp Val Tyr Asn Glu Asn Arg Lys Ala Met Arg Glu 165 170 175 Phe Thr Asp Ile Ala Ala Asn Tyr Leu Asn Ile Ile Asp Pro Arg Lys 180 185 190 Arg His Glu Ile Ile Lys Ala Arg Phe Phe Met Glu Lys Ser Lys His 195 200 205 Thr Ala Leu Val Lys Glu Leu Asn Ser Glu Leu Lys Ser Leu Pro Val 210 215 220 Glu Asp Phe Thr Gly Lys Lys Val Ile Leu Thr Gly Ile Met Ala Glu 225 230 235 240 Pro Asn Glu Val Leu Asp Ile Leu Lys Glu Asn Asp Phe Ala Val Val 245 250 255 Ala Asp Asp Leu Ala Gln Glu Ser Arg Leu Phe Arg Ile Asp Val Pro 260 265 270 Ala Gly Pro Asp Pro Leu Tyr Arg Leu Ala Lys Trp Trp Gln Glu Phe 275 280 285 Asp Gly Cys Ser Leu Ala Val Asp Thr Lys Lys Leu Arg Gly Pro Met 290 295 300 Leu Met Asn Met Val Asn Val Asp Lys Ala Asp Ala Val Val Val Cys 305 310 315 320 Met Met Lys Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Tyr 325 330 335 Arg Gln Phe Glu Glu Ala Gly Ile Lys Ser Leu Phe Ile Glu Ile Asp 340 345 350 Leu Glu Pro Thr Ser Phe Glu Gln Thr Lys Thr Arg Val Gln Ser Phe 355 360 365 Arg Glu Met Leu 370 <210> 107 <211> 266 <212> PRT <213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) <400> 107 Met Tyr Thr Met Gly Ile Asp Ile Gly Ser Ser Ser Ser Lys Val Val 1 5 10 15 Ile Leu Glu Asp Gly Val Asn Leu Ile Ala Gly Glu Val Ile Gln Ile 20 25 30 Gly Thr Gly Ser Thr Gly Pro Lys Arg Val Leu Glu Glu Ala Leu Ala 35 40 45 Lys Thr Gly Leu Thr Leu Ala Asp Met Ala Lys Ile Ile Ala Thr Gly 50 55 60 Tyr Gly Arg Ser Ser Val Glu Val Ser Asp Lys Gln Ile Ser Glu Ile 65 70 75 80 Ser Cys Gln Ala Lys Gly Val Tyr Phe Leu Val Pro Thr Ala Lys Leu 85 90 95 Ile Ile Asp Ile Gly Gly Gln Asp Val Lys Ala Ile Arg Leu Asp Arg 100 105 110 Ile Gly Gly Val Arg Gln Phe Phe Met Asn Asp Lys Cys Ala Ala Gly 115 120 125 Thr Gly Arg Phe Leu Asp Val Met Ser Arg Val Leu Glu Val Asp Leu 130 135 140 Asp Glu Met Ala Glu Tyr Asp Ala Arg Ala Thr Glu Pro Ala Thr Val 145 150 155 160 Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Gln Leu 165 170 175 Ala Asn Gly Val Ala Lys Glu Asn Ile Ile Ala Gly Val His Gln Ser 180 185 190 Val Ala Ser Lys Ala Cys Gly Leu Ala Tyr Arg Cys Gly Val Glu Glu 195 200 205 Asp Val Val Met Cys Gly Gly Val Ala Lys Asp Leu Gly Val Val Arg 210 215 220 Ala Ile Ser Lys Glu Leu Lys Lys Pro Val Ile Val Ala Pro Asn Pro 225 230 235 240 Gln Ile Thr Ala Ala Leu Gly Ala Ala Leu Phe Ala Tyr Glu Glu Val 245 250 255 Met Glu Ala Asn Lys Leu Arg Lys Glu Val 260 265 <210> 108 <211> 411 <212> PRT <213> Peptostreptococcus anaerobius CAG:621 <400> 108 Met Ser Asn Thr Gly Ala Val Glu Glu Lys Pro Ala Lys Val Leu Leu 1 5 10 15 Gly Glu Ile Val Ala Lys His Tyr Lys Glu Ala Trp Glu Ala Lys Glu 20 25 30 Arg Gly Glu Lys Val Gly Trp Cys Ala Ser Asn Phe Pro Gln Glu Ile 35 40 45 Phe Glu Thr Met Asp Ile Lys Val Val Phe Pro Glu Asn Gln Ala Ala 50 55 60 Ala Ile Ser Ala Lys Gly Gly Gly Gln Arg Met Cys Glu Ile Ala Glu 65 70 75 80 Asn Glu Gly Tyr Ser Asn Asp Ile Cys Ala Tyr Ala Arg Ile Ser Leu 85 90 95 Ala Tyr Met Asp Val Lys Asp Ala Pro Glu Leu Asn Met Pro Gln Pro 100 105 110 Asp Phe Val Ala Cys Cys Asn Asn Ile Cys Asn Cys Met Ile Lys Trp 115 120 125 Tyr Glu Asn Ile Ala Lys Glu Leu Asn Ile Pro Leu Ile Leu Val Asp 130 135 140 Val Pro Tyr Asn Asn Asp Tyr Glu Ala Gly Asp Asp Arg Val Glu Tyr 145 150 155 160 Leu Arg Gly Gln Phe Asp His Ala Ile Lys Gln Leu Glu Asp Leu Thr 165 170 175 Gly Lys Lys Trp Asp Glu Lys Lys Phe Glu Glu Val Met Ala Ile Ser 180 185 190 Gln Arg Thr Gly Arg Ala Trp Leu Lys Ala Thr Gly Tyr Ala Lys Tyr 195 200 205 Thr Pro Ser Pro Phe Ser Gly Phe Asp Val Phe Asn His Met Ala Val 210 215 220 Ala Val Cys Ala Arg Gly Lys Glu Glu Ser Ala Ile Ala Phe Glu Lys 225 230 235 240 Leu Ala Glu Glu Phe Asp Glu Asn Val Lys Thr Gly Lys Ser Thr Phe 245 250 255 Lys Gly Glu Glu Lys Tyr Arg Val Leu Phe Glu Gly Ile Ala Cys Trp 260 265 270 Pro His Leu Arg His Thr Phe Lys Gln Leu Lys Asp Ser Gly Val Asn 275 280 285 Val Cys Gly Thr Val Tyr Ala Asp Ala Phe Gly Tyr Ile Tyr Asp Asn 290 295 300 Thr Tyr Glu Leu Met Gln Ala Tyr Cys Gly Thr Pro Asn Ala Ile Ser 305 310 315 320 Tyr Glu Arg Ser Leu Asp Met Arg Leu Lys Val Ile Glu Glu Asn Asn 325 330 335 Ile Asp Gly Met Leu Ile His Ile Asn Arg Ser Cys Lys Gln Trp Ser 340 345 350 Gly Ile Met Tyr Glu Met Glu Arg Glu Ile Arg Glu Arg Thr Gly Ile 355 360 365 Pro Thr Ala Thr Phe Asp Gly Asp Gln Ala Asp Pro Arg Asn Phe Ser 370 375 380 Glu Ala Gln Tyr Asp Thr Arg Val Gln Gly Leu Ile Glu Val Met Glu 385 390 395 400 Ala Asn Lys Ala Ala Lys Met Lys Glu Glu Asn 405 410 <210> 109 <211> 372 <212> PRT <213> Peptostreptococcus anaerobius CAG:621 <400> 109 Met Ser Asn Leu Glu Glu Leu Phe Gly Lys Leu Ala Val Cys Pro Leu 1 5 10 15 Glu Gln Ile Asp Lys Tyr Val Ala Asp Gly Lys Lys Val Ile Gly Cys 20 25 30 Ala Pro Val Tyr Ala Pro Glu Glu Leu Val Tyr Ala Ser Gly Met Ile 35 40 45 Pro Met Ala Ile Trp Gly Ala Glu Gly Glu Val Thr Leu Ala Lys Glu 50 55 60 Tyr Phe Pro Ala Phe Tyr Val Ser Ile Ile Leu Arg Leu Leu Asp Leu 65 70 75 80 Gly Leu Glu Gly Lys Leu Asp Lys Met Ser Gly Met Ile Leu Pro Gly 85 90 95 Leu Ser Asp Gly Leu Lys Gly Leu Ser Gln Asn Trp Lys Arg Ala Val 100 105 110 Lys Asn Val Pro Ala Leu Tyr Ile Gly Tyr Gly Gln Asn Arg Lys Ile 115 120 125 Glu Ala Gly Ile Val Tyr Asn Ala Arg Gln Tyr Glu Lys Leu Lys Val 130 135 140 Gln Leu Glu Glu Ile Ala Gly Lys Lys Ile Glu Asp Ala Gln Ile Glu 145 150 155 160 Glu Ala Ile Val Leu Tyr Asn Lys His Arg Lys Ala Met Gln Ala Phe 165 170 175 Ser Asp Leu Ala Ala Lys His Leu Asn Thr Val Thr Pro Ser Leu Arg 180 185 190 Ala Lys Val Met Ser Ser Ala Cys Leu Met Asp Lys Ala Glu His Leu 195 200 205 Glu Ile Val Glu Ala Ile Asn Ala Glu Leu Ser Ala Met Pro Glu Glu 210 215 220 Lys Phe Asp Gly Lys Lys Ile Val Thr Thr Gly Leu Leu Ala Asn Ser 225 230 235 240 Pro Glu Ile Leu Lys Ile Phe Glu Glu Phe Lys Leu Gly Ile Val Ala 245 250 255 Asp Asn Ile Asn His Glu Ser Gly Gln Phe Asp Tyr Leu Val Asp Glu 260 265 270 Ala Thr Gly Asn Pro Ile Lys Ala Leu Ser Lys Trp Ile Ser Asp Ile 275 280 285 Glu Gly Ser Thr Leu Leu Tyr Asp Pro Glu Lys Leu Arg Gly Gln Ile 290 295 300 Ile Ile Asp Lys Ala Lys Lys Tyr Asp Ala Asp Gly Val Val Tyr Leu 305 310 315 320 Leu Ser Lys Phe Ser Asp Ser Asp Glu Phe Asp Tyr Pro Ile Ile Arg 325 330 335 Lys Gln Leu Glu Glu Ala Gly Tyr Met His Ile Leu Val Glu Val Asp 340 345 350 Gln Gln Met Thr Asn Phe Glu Gln Ala Lys Thr Ala Leu Gln Thr Phe 355 360 365 Ala Asp Met Ile 370 <210> 110 <211> 263 <212> PRT <213> Peptostreptococcus anaerobius CAG:621 <400> 110 Met Ser Asp Ile Tyr Thr Met Gly Ile Asp Ile Gly Ser Thr Ser Ser 1 5 10 15 Lys Cys Val Val Leu Lys Asn Gly Lys Asp Leu Val Ser Ser Gly Val 20 25 30 Val Asn Leu Gly Ala Gly Thr Lys Gly Ala Asp Gln Val Ile Glu Lys 35 40 45 Val Leu Ala Asp Cys Gly Ile Lys Phe Glu Asp Leu Asn Val Ile Val 50 55 60 Ser Thr Gly Tyr Gly Arg Asn Ser Tyr Asp Ser Ala Lys Lys Thr Met 65 70 75 80 Ser Glu Leu Ser Cys His Ala Lys Gly Gly Thr Tyr Ile Phe Gly Pro 85 90 95 Val Arg Thr Ile Ile Asp Ile Gly Gly Gln Asp Ile Lys Val Leu Lys 100 105 110 Leu Asn Asp Lys Gly Met Met Thr Asn Phe Leu Met Asn Asp Lys Cys 115 120 125 Ala Ala Gly Thr Gly Arg Phe Leu Glu Val Met Ala Gly Val Leu Asp 130 135 140 Val Lys Leu Ala Glu Leu Gly Asp Leu Asp Lys Leu Ala Thr Glu Lys 145 150 155 160 Thr Pro Ile Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile 165 170 175 Ser Cys Met Ala Lys Lys Ile Pro Ile Pro Asn Ile Ile Arg Gly Ile 180 185 190 His Ala Ser Val Ala Thr Arg Val Ala Gly Leu Ala Lys Arg Gly Gly 195 200 205 Leu Thr Thr Pro Val Ala Met Thr Gly Gly Val Thr Lys Asn Ser Gly 210 215 220 Ile Val Arg Ala Leu Ser Glu Glu Leu Glu Thr Asp Ile Met Ile Ser 225 230 235 240 Glu Ile Ser Gln Leu Ala Gly Ala Ile Gly Ala Ala Leu Tyr Ala Tyr 245 250 255 Asp Glu Tyr Leu Lys Glu Asn 260 <210> 111 <211> 258 <212> PRT <213> Chloroflexus aggregans (strain MD-66 / DSM 9485) <400> 111 Met Ser Asp Glu Thr Leu Val Leu Ser Thr Ile Glu Gly Pro Val Ala 1 5 10 15 Ile Leu Thr Leu Asn Arg Pro Gln Ala Leu Asn Ala Leu Ser Pro Ala 20 25 30 Leu Ile Asp Ala Leu Ile Arg His Leu Glu His Cys Asp Asn Asp Asp 35 40 45 Thr Ile Arg Val Ile Ile Ile Thr Gly Ala Gly Arg Ala Phe Ala Ala 50 55 60 Gly Ala Asp Ile Lys Ala Met Ala Asp Ala Thr Pro Ile Asp Met Leu 65 70 75 80 Thr Thr Asp Met Ile Ala Arg Trp Ala Arg Ile Ala Ala Val Arg Lys 85 90 95 Pro Val Ile Ala Ala Val Asn Gly Phe Ala Leu Gly Gly Gly Cys Glu 100 105 110 Leu Ala Met Met Cys Asp Ile Ile Leu Ala Ser Glu Thr Ala Gln Phe 115 120 125 Gly Gln Pro Glu Ile Asn Ile Gly Ile Ile Pro Gly Ala Gly Gly Thr 130 135 140 Gln Arg Leu Thr Arg Ala Ile Gly Pro Tyr Arg Ala Met Glu Met Val 145 150 155 160 Leu Thr Gly Ala Thr Ile Ser Ala Gln Glu Ala Tyr Ala Tyr Gly Leu 165 170 175 Val Asn Arg Val Cys Pro Pro Asp Ser Leu Leu Asp Glu Ala Arg Arg 180 185 190 Leu Ala Gln Thr Ile Ala Ala Lys Pro Pro Leu Ala Val Arg Leu Ala 195 200 205 Lys Glu Ala Val Arg Ala Ala Ala Glu Thr Thr Val Arg Glu Gly Leu 210 215 220 Ala Ile Glu Leu Arg Asn Phe Tyr Leu Leu Phe Ala Ser Ala Asp Gln 225 230 235 240 Lys Glu Gly Met Arg Ala Phe Ile Glu Lys Arg Thr Ala Asn Phe Ser 245 250 255 Gly Arg <210> 112 <211> 257 <212> PRT <213> Marivirga tractuosa <400> 112 Met Glu Phe Ile Lys Val Asn Thr Gln Tyr Lys Lys His Ile Ala Leu 1 5 10 15 Ile Asn Leu Asn Arg Pro Lys Glu Leu Asn Ala Leu Asn Leu Gln Leu 20 25 30 Met Thr Glu Leu Lys Asp Thr Leu Lys Val Leu Asp Glu Asp Glu Asn 35 40 45 Val Arg Val Ile Ile Leu Thr Gly Asn Glu Lys Ala Phe Ala Ala Gly 50 55 60 Ala Asp Ile Lys Gln Met Ala Gly Lys Thr Ala Ile Asp Met Leu Asn 65 70 75 80 Val Asp Gln Phe Ser Thr Trp Asp Gln Ile Lys Lys Thr Lys Lys Pro 85 90 95 Leu Ile Ala Ala Val Ser Gly Phe Ala Leu Gly Gly Gly Cys Glu Leu 100 105 110 Ala Met Thr Cys Asp Met Ile Val Ala Ser Glu Ser Ala Lys Phe Gly 115 120 125 Gln Pro Glu Ile Lys Ile Gly Val Met Pro Gly Ala Gly Gly Thr Gln 130 135 140 Arg Leu Thr Arg Ala Ile Gly Lys Ala Lys Ala Met Glu Leu Val Leu 145 150 155 160 Thr Gly Asn Phe Ile Ser Ala Glu Glu Ala Met His Tyr Gly Leu Val 165 170 175 Asn Lys Val Val Pro Thr Glu Met Tyr Leu Glu Ala Ala Ala Glu Leu 180 185 190 Ala Glu Gln Ile Ala Gln Met Ser Pro Val Ala Ala Lys Leu Ala Lys 195 200 205 Glu Ser Val Asn Arg Ala Phe Glu Thr His Leu Asp Glu Gly Leu His 210 215 220 Phe Glu Arg Lys Asn Phe Tyr Leu Thr Phe Ala Ser Glu Asp Gln Thr 225 230 235 240 Glu Gly Met Glu Ala Phe Val Glu Lys Arg Lys Pro Glu Phe Lys Gly 245 250 255 Lys <210> 113 <211> 257 <212> PRT <213> Marinithermus hydrothermalis (strain DSM 14884/JCM 11576/T1) <400> 113 Met Tyr Glu Asn Leu Ile Val Glu Thr Leu Glu Gly Gly Val Gly Leu 1 5 10 15 Ile Arg Ile His Arg Pro Lys Arg Leu Asn Ala Leu Asn Gln Ala Thr 20 25 30 Met Asp Glu Ile Val Arg Ala Val Arg Ala Phe Glu Ala Asp Asp Ala 35 40 45 Val Arg Ala Ile Val Leu Thr Gly Asp Glu Arg Ala Phe Ala Ala Gly 50 55 60 Ala Asp Val Thr Glu Met Asp Gly Ala Asn Val Pro Glu Met Leu Ser 65 70 75 80 Gly Tyr Arg Phe Glu Gln Trp Glu Thr Leu Arg Arg Thr Thr Lys Pro 85 90 95 Leu Ile Ala Ala Val Ser Gly Phe Ala Leu Gly Gly Gly Leu Glu Leu 100 105 110 Ala Met Leu Cys Asp Ile Ile Val Ala Ser Glu Thr Ala Arg Leu Gly 115 120 125 Gln Pro Glu Ile Asn Leu Gly Ile Met Pro Gly Ala Gly Gly Thr Gln 130 135 140 Arg Leu Thr Arg Gln Val Gly Lys Tyr Leu Ala Met Glu Met Val Leu 145 150 155 160 Thr Gly Arg Met Leu Thr Ala Glu Glu Ala Tyr Arg His Gly Leu Val 165 170 175 Asn Arg Val Val Pro Val Glu Phe Tyr Leu Glu Glu Ala Ile Gln Ile 180 185 190 Ala Arg Glu Ile Ala Lys Lys Ala Pro Val Ala Val Arg Leu Ala Lys 195 200 205 Asp Ala Ile Leu Lys Ala Glu Asp Thr Pro Leu Glu Val Gly Leu Ala 210 215 220 Tyr Glu Arg His Asn Phe Tyr Leu Leu Phe Gly Thr Glu Asp Lys Gln 225 230 235 240 Glu Gly Ile Arg Ala Phe Leu Glu Lys Arg Lys Pro Glu Trp Lys Gly 245 250 255 Arg <210> 114 <211> 259 <212> PRT <213> Chitinophaga pinensis (strain ATCC 43595/DSM 2588/NCIB11800/UQM 2034) <400> 114 Met Gln Pro Gln Phe Ile Ile Ile His Arg Gln Val Ala Pro Tyr Val 1 5 10 15 Ala His Ile Gln Leu Asn Arg Pro Lys Glu Leu Asn Ala Leu Asn Leu 20 25 30 Glu Leu Met Ile Glu Leu Arg Asp Ala Leu Lys Met Leu Asp Ala Asp 35 40 45 Asp Asn Val Arg Ala Ile Val Ile Ser Gly Asn Glu Lys Ala Phe Ala 50 55 60 Ala Gly Ala Asp Ile Lys Gln Met Ala Gly Lys Thr Ala Met Asp Met 65 70 75 80 Tyr Asn Ile Asp Gln Phe Ser Thr Trp Asp Thr Ile Lys Lys Thr Lys 85 90 95 Lys Pro Leu Ile Ala Ala Val Ser Gly Phe Ala Leu Gly Gly Gly Cys 100 105 110 Glu Leu Val Met Leu Cys Asp Met Ile Val Ala Ser Glu Thr Ala Arg 115 120 125 Phe Gly Gln Pro Glu Ile Lys Ile Gly Val Met Pro Gly Ala Gly Gly 130 135 140 Thr Gln Arg Leu Thr Arg Ala Val Gly Lys Ala Leu Ala Met Glu Met 145 150 155 160 Val Leu Thr Gly Arg Phe Ile Thr Ala Gln Glu Ala Ala Arg Ala Gly 165 170 175 Leu Ile Asn Arg Val Ile Pro Val Glu Leu Phe Leu Gln Glu Ala Ile 180 185 190 Arg Leu Ala Thr Glu Val Ala Ala Leu Ser Pro Leu Ala Val Lys Met 195 200 205 Ala Lys Glu Ser Val Leu Lys Ala Phe Asp Ser Ser Leu Glu Glu Gly 210 215 220 Leu His Phe Glu Arg Lys Asn Phe Tyr Leu Leu Phe Ala Ser Glu Asp 225 230 235 240 Gln Lys Glu Gly Met Gln Ala Phe Val Asp Lys Arg Ser Pro Val Phe 245 250 255 Lys Gly Lys <210> 115 <211> 258 <212> PRT <213> Megasphaera elsdenii DSM 20460 <400> 115 Met Tyr Thr Leu Gly Ile Asp Val Gly Ser Ser Ser Ser Lys Ala Val 1 5 10 15 Ile Leu Glu Asp Gly Lys Lys Ile Val Ala His Ala Val Val Glu Ile 20 25 30 Gly Thr Gly Ser Thr Gly Pro Glu Arg Val Leu Asp Glu Val Phe Lys 35 40 45 Asp Thr Asn Leu Lys Ile Glu Asp Met Ala Asn Ile Ile Ala Thr Gly 50 55 60 Tyr Gly Arg Phe Asn Val Asp Cys Ala Lys Gly Glu Val Ser Glu Ile 65 70 75 80 Thr Cys His Ala Lys Gly Ala Leu Phe Glu Cys Pro Gly Thr Thr Thr 85 90 95 Ile Leu Asp Ile Gly Gly Gln Asp Val Lys Ser Ile Lys Leu Asn Gly 100 105 110 Gln Gly Leu Val Met Gln Phe Ala Met Asn Asp Lys Cys Ala Ala Gly 115 120 125 Thr Gly Arg Phe Leu Asp Val Met Ser Lys Val Leu Glu Ile Pro Met 130 135 140 Ser Glu Met Gly Asp Trp Tyr Phe Lys Ser Lys His Pro Ala Ala Val 145 150 155 160 Ser Ser Thr Cys Thr Val Phe Ala Glu Ser Glu Val Ile Ser Leu Leu 165 170 175 Ser Lys Asn Val Pro Lys Glu Asp Ile Val Ala Gly Val His Gln Ser 180 185 190 Ile Ala Ala Lys Ala Cys Ala Leu Val Arg Arg Val Gly Val Gly Glu 195 200 205 Asp Leu Thr Met Thr Gly Gly Gly Ser Arg Asp Pro Gly Val Val Asp 210 215 220 Ala Val Ser Lys Glu Leu Gly Ile Pro Val Arg Val Ala Leu His Pro 225 230 235 240 Gln Ala Val Gly Ala Leu Gly Ala Ala Leu Ile Ala Tyr Asp Lys Ile 245 250 255 Lys Lys <210> 116 <211> 428 <212> PRT <213> Megasphaera elsdenii DSM 20460 <400> 116 Met Ser Glu Glu Lys Thr Val Asp Ile Glu Ser Met Ser Ser Lys Glu 1 5 10 15 Ala Leu Gly Tyr Phe Leu Pro Lys Val Asp Glu Asp Ala Arg Lys Ala 20 25 30 Lys Lys Glu Gly Arg Leu Val Cys Trp Ser Ala Ser Val Ala Pro Pro 35 40 45 Glu Phe Cys Thr Ala Met Asp Ile Ala Ile Val Tyr Pro Glu Thr His 50 55 60 Ala Ala Gly Ile Gly Ala Arg His Gly Ala Pro Ala Met Leu Glu Val 65 70 75 80 Ala Glu Asn Lys Gly Tyr Asn Gln Asp Ile Cys Ser Tyr Cys Arg Val 85 90 95 Asn Met Gly Tyr Met Glu Leu Leu Lys Gln Gln Ala Leu Thr Gly Glu 100 105 110 Thr Pro Glu Val Leu Lys Asn Ser Pro Ala Ser Pro Ile Pro Leu Pro 115 120 125 Asp Val Val Leu Thr Cys Asn Asn Ile Cys Asn Thr Leu Leu Lys Trp 130 135 140 Tyr Glu Asn Leu Ala Lys Glu Leu Asn Val Pro Leu Ile Asn Ile Asp 145 150 155 160 Val Pro Phe Asn His Glu Phe Pro Val Thr Lys His Ala Lys Gln Tyr 165 170 175 Ile Val Gly Glu Phe Lys His Ala Ile Lys Gln Leu Glu Asp Leu Cys 180 185 190 Gly Arg Pro Phe Asp Tyr Asp Lys Phe Phe Glu Val Gln Lys Gln Thr 195 200 205 Gln Arg Ser Ile Ala Ala Trp Asn Lys Ile Ala Thr Tyr Phe Gln Tyr 210 215 220 Lys Pro Ser Pro Leu Asn Gly Phe Asp Leu Phe Asn Tyr Met Gly Leu 225 230 235 240 Ala Val Ala Ala Arg Ser Leu Asn Tyr Ser Glu Ile Thr Phe Asn Lys 245 250 255 Phe Leu Lys Glu Leu Asp Glu Lys Val Ala Asn Lys Lys Trp Ala Phe 260 265 270 Gly Glu Asn Glu Lys Ser Arg Val Thr Trp Glu Gly Ile Ala Val Trp 275 280 285 Ile Ala Leu Gly His Thr Phe Lys Glu Leu Lys Gly Gln Gly Ala Leu 290 295 300 Met Thr Gly Ser Ala Tyr Pro Gly Met Trp Asp Val Ser Tyr Glu Pro 305 310 315 320 Gly Asp Leu Glu Ser Met Ala Glu Ala Tyr Ser Arg Thr Tyr Ile Asn 325 330 335 Cys Cys Leu Glu Gln Arg Gly Ala Val Leu Glu Lys Val Val Arg Asp 340 345 350 Gly Lys Cys Asp Gly Leu Ile Met His Gln Asn Arg Ser Cys Lys Asn 355 360 365 Met Ser Leu Leu Asn Asn Glu Gly Gly Gln Arg Ile Gln Lys Asn Leu 370 375 380 Gly Val Pro Tyr Val Ile Phe Asp Gly Asp Gln Thr Asp Ala Arg Asn 385 390 395 400 Phe Ser Glu Ala Gln Phe Asp Thr Arg Val Glu Ala Leu Ala Glu Met 405 410 415 Met Ala Asp Lys Lys Ala Asn Glu Gly Gly Asn His 420 425 <210> 117 <211> 372 <212> PRT <213> Megasphaera elsdenii DSM 20460 <400> 117 Met Ser Gln Ile Asp Glu Leu Ile Ser Lys Leu Gln Glu Val Ser Asn 1 5 10 15 His Pro Gln Lys Thr Val Leu Asn Tyr Lys Lys Gln Gly Lys Gly Leu 20 25 30 Val Gly Met Met Pro Tyr Tyr Ala Pro Glu Glu Ile Val Tyr Ala Ala 35 40 45 Gly Tyr Leu Pro Val Gly Met Phe Gly Ser Gln Asn Pro Gln Ile Ser 50 55 60 Ala Ala Arg Thr Tyr Leu Pro Pro Phe Ala Cys Ser Leu Met Gln Ala 65 70 75 80 Asp Met Glu Leu Gln Leu Asn Gly Thr Tyr Asp Cys Leu Asp Ala Val 85 90 95 Ile Phe Ser Val Pro Cys Asp Thr Leu Arg Cys Met Ser Gln Lys Trp 100 105 110 His Gly Lys Ala Pro Val Ile Val Phe Thr Gln Pro Gln Asn Arg Lys 115 120 125 Ile Arg Pro Ala Val Asp Phe Leu Lys Ala Glu Tyr Glu His Val Arg 130 135 140 Thr Glu Leu Glu Arg Ile Leu Asn Val Lys Ile Ser Asp Leu Ala Ile 145 150 155 160 Gln Glu Ala Ile Lys Val Tyr Asn Glu Asn Arg Gln Val Met Arg Glu 165 170 175 Phe Cys Asp Val Ala Ala Gln Tyr Pro Gln Ile Phe Thr Pro Val Lys 180 185 190 Arg His Asp Val Ile Lys Ala Arg Trp Phe Met Asp Lys Ala Glu His 195 200 205 Thr Ala Leu Val Arg Glu Leu Ile Asp Ala Val Lys Lys Glu Pro Val 210 215 220 Gln Pro Trp Asn Gly Lys Lys Val Ile Leu Ser Gly Ile Met Ala Glu 225 230 235 240 Pro Asp Glu Phe Leu Asp Ile Phe Ser Glu Phe Asn Ile Ala Val Val 245 250 255 Ala Asp Asp Leu Ala Gln Glu Ser Arg Gln Phe Arg Thr Asp Val Pro 260 265 270 Ser Gly Ile Asp Pro Leu Glu Gln Leu Ala Gln Gln Trp Gln Asp Phe 275 280 285 Asp Gly Cys Pro Leu Ala Leu Asn Glu Asp Lys Pro Arg Gly Gln Met 290 295 300 Leu Ile Asp Met Thr Lys Lys Tyr Asn Ala Asp Ala Val Val Ile Cys 305 310 315 320 Met Met Arg Phe Cys Asp Pro Glu Glu Phe Asp Tyr Pro Ile Tyr Lys 325 330 335 Pro Glu Phe Glu Ala Ala Gly Val Arg Tyr Thr Val Leu Asp Leu Asp 340 345 350 Ile Glu Ser Pro Ser Leu Glu Gln Leu Arg Thr Arg Ile Gln Ala Phe 355 360 365 Ser Glu Ile Leu 370 <210> 118 <211> 258 <212> PRT <213> Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) <400> 118 Met Ser Glu Glu Ser Leu Val Leu Ser Thr Ile Glu Gly Pro Ile Ala 1 5 10 15 Ile Leu Thr Leu Asn Arg Pro Gln Ala Leu Asn Ala Leu Ser Pro Ala 20 25 30 Leu Ile Asp Asp Leu Ile Arg His Leu Glu Ala Cys Asp Ala Asp Asp 35 40 45 Thr Ile Arg Val Ile Ile Ile Thr Gly Ala Gly Arg Ala Phe Ala Ala 50 55 60 Gly Ala Asp Ile Lys Ala Met Ala Asn Ala Thr Pro Ile Asp Met Leu 65 70 75 80 Thr Ser Gly Met Ile Ala Arg Trp Ala Arg Ile Ala Ala Val Arg Lys 85 90 95 Pro Val Ile Ala Ala Val Asn Gly Tyr Ala Leu Gly Gly Gly Cys Glu 100 105 110 Leu Ala Met Met Cys Asp Ile Ile Ile Ala Ser Glu Asn Ala Gln Phe 115 120 125 Gly Gln Pro Glu Ile Asn Leu Gly Ile Ile Pro Gly Ala Gly Gly Thr 130 135 140 Gln Arg Leu Thr Arg Ala Leu Gly Pro Tyr Arg Ala Met Glu Leu Ile 145 150 155 160 Leu Thr Gly Ala Thr Ile Ser Ala Gln Glu Ala Leu Ala His Gly Leu 165 170 175 Val Cys Arg Val Cys Pro Pro Glu Ser Leu Leu Asp Glu Ala Arg Arg 180 185 190 Ile Ala Gln Thr Ile Ala Thr Lys Ser Pro Leu Ala Val Gln Leu Ala 195 200 205 Lys Glu Ala Val Arg Met Ala Ala Glu Thr Thr Val Arg Glu Gly Leu 210 215 220 Ala Ile Glu Leu Arg Asn Phe Tyr Leu Leu Phe Ala Ser Ala Asp Gln 225 230 235 240 Lys Glu Gly Met Gln Ala Phe Ile Glu Lys Arg Ala Pro Asn Phe Ser 245 250 255 Gly Arg <210> 119 <211> 258 <212> PRT <213> Ruegeria pomeroyi DSS-3 <400> 119 Met Ala Phe Glu Thr Ile Ile Val Glu Val Glu Asp His Val Ala Leu 1 5 10 15 Ile Arg Leu Asn Arg Pro Asp Ala Leu Asn Ala Leu Asn Thr Gln Leu 20 25 30 Leu Gly Glu Leu Cys Thr Ala Leu Glu Glu Ala Asp Gly Asn Asp Lys 35 40 45 Val Arg Cys Ile Val Ile Thr Gly Ser Asp Lys Ala Phe Ala Ala Gly 50 55 60 Ala Asp Ile Arg Glu Met Ser Gln Lys Thr Tyr Val Glu Val Tyr Ser 65 70 75 80 Glu Asn Leu Phe Ala Ala Ala Asn Asp Arg Val Ser Ala Ile Arg Lys 85 90 95 Pro Ile Ile Ala Ala Val Ala Gly Tyr Ala Leu Gly Gly Gly Cys Glu 100 105 110 Leu Ala Met Leu Cys Asp Phe Ile Ile Ala Ala Asp Thr Ala Lys Phe 115 120 125 Gly Gln Pro Glu Ile Asn Leu Gly Val Ile Ala Gly Ile Gly Gly Thr 130 135 140 Gln Arg Leu Thr Arg Leu Val Gly Lys Ser Lys Ser Met Asp Leu Asn 145 150 155 160 Leu Thr Gly Arg Phe Met Asp Ala Glu Glu Ala Glu Arg Ala Gly Leu 165 170 175 Val Ser Arg Val Val Pro Ala Lys Lys Leu Val Glu Glu Ala Leu Ser 180 185 190 Ala Ala Gln Lys Ile Ala Glu Lys Ser Met Ile Ser Ala Tyr Ala Val 195 200 205 Lys Glu Ala Val Asn Arg Ser Tyr Glu Thr Thr Leu Ser Glu Gly Leu 210 215 220 Leu Phe Glu Arg Arg Val Phe His Ser Met Phe Ala Thr Glu Asp Gln 225 230 235 240 Lys Glu Gly Met Ala Ala Phe Leu Glu Lys Arg Ala Ala Gln Phe Arg 245 250 255 Asp Lys <210> 120 <211> 900 <212> DNA <213> Dictyostelium discoideum (Slime mold) <400> 120 atgattaata gattattttc aattaataat attaaaaatg gatcaaaatt ttttagttca 60 tcaacaacag ttgaaactaa acaaccatta gttttattag aaaaacattt agtaaatgga 120 aaatatacag gtattcaaat tgttaaatta aataaaccaa aacaattgaa tgcattaaca 180 tttgaaatgg gagttgatta taagaaggtg gtggatacat tagcagaaga taaagatttg 240 aaatgtgttg tattgacagg tgaaggtaag gcattttcgg caggtggtga tttagatttc 300 ttaattgaaa gaactaaaga cacaccagaa aacaatcaaa gaattatgga aagattctat 360 agaacatttt tatatattcg ttcattacca gtaccaatca tttctgcaat caatggtgca 420 gcaattggtg caggtttctg tttagcttta gcaactgata ttcgtgtcgt tagtaataaa 480 gcaccagtgg gtttaacatt caccaaatta ggtattcatc caggtatggg tgtaactcat 540 tcaattacaa atatagttgg tcaagatgtt gcatcctata tgttattatc aagtgatatt 600 atcaaaggtg atgaagctca aagattaggt ttagttttaa aatcggttga atctgatcaa 660 gttttaccaa ctgctttaaa tctcgctgaa acaatctcaa aaaattcaac tatcgctgta 720 aactctacaa caaaaacttt acgtaataaa tataattcag atttagataa aagtttaact 780 cgtgaagctg atgctcaaag tcaatgttgg gcttcaaaag atatagttga aggtatttta 840 gcaattagag aaagtagaga tccaaaacat aattatttat tatttgatga tcaaaaataa 900 900 <210> 121 <211> 786 <212> DNA <213> Clostridium acetobutylicum <400> 121 atggaactaa acaatgtcat ccttgaaaag gaaggtaaag ttgctgtagt taccattaac 60 agacctaaag cattaaatgc gttaaatagt gatacactaa aagaaatgga ttatgttata 120 ggtgaaattg aaaatgatag cgaagtactt gcagtaattt taactggagc aggagaaaaa 180 tcatttgtag caggagcaga tatttctgag atgaaggaaa tgaataccat tgaaggtaga 240 aaattcggga tacttggaaa taaagtgttt agaagattag aacttcttga aaagcctgta 300 atagcagctg ttaatggttt tgctttagga ggcggatgcg aaatagctat gtcttgtgat 360 ataagaatag cttcaagcaa cgcaagattt ggtcaaccag aagtaggtct cggaataaca 420 cctggttttg gtggtacaca aagactttca agattagttg gaatgggcat ggcaaagcag 480 cttatattta ctgcacaaaa tataaaggca gatgaagcat taagaatcgg acttgtaaat 540 aaggtagtag aacctagtga attaatgaat acagcaaaag aaattgcaaa caaaattgtg 600 agcaatgctc cagtagctgt taagttaagc aaacaggcta ttaatagagg aatgcagtgt 660 gatattgata ctgctttagc atttgaatca gaagcatttg gagaatgctt ttcaacagag 720 gatcaaaagg atgcaatgac agctttcata gagaaaagaa aaattgaagg cttcaaaaat 780 agatag 786 <210> 122 <211> 468 <212> DNA <213> Clostridium difficile <400> 122 aatagtaaaa aagtagtgat agctgctgta aacggatttg ctttaggtgg atgtgaactt 60 gcaatggcat gtgatataag aattgcatct gctaaagcta aatttggtca gccagaagta 120 actcttggaa taactccagg atatggagga actcaaaggc ttacaagatt ggttggaatg 180 gcaaaagcaa aagaattaat ctttacaggt caagttataa aagctgatga agctgaaaaa 240 atagggctag taaatagagt cgttgagcca gacattttaa tagaagaagt tgagaaatta 300 gctaagataa tagctaaaaa tgctcagctt gcagttagat actctaaaga agcaatacaa 360 cttggtgctc aaactgatat aaatactgga atagatatag aatctaattt atttggtctt 420 tgtttttcaa ctaaagacca aaaagaagga attgtcagct ttcgttga 468 <210> 123 <211> 777 <212> DNA <213> Clostridium pasteurianum <400> 123 atgggaaata ttatctttga agaagaagat ggaatagaaa aagttacaat taacagacct 60 aaagctctta atgcattaaa tagtgaaaca ttaaaagaac ttggtacagt aataaatgac 120 atatctgtaa acgatggaat aaaagctgta ataataacag gttcgggatc aaaagctttt 180 gtagctggtg cagatatagc tgaaatgagt actctaaatt caatagaggc aacaaatttt 240 tcaagacttg cccaaaatgt attttcacaa atagaaaatc tacctaaatt agtagtagca 300 gcagttaacg gttttgctct tggaggagga tgtgagcttg caatggcttg tgatgtaagg 360 tttgcttcaa aaaaagctaa atttggtcaa ccagaagtta atttaggaat attgccaagt 420 ttcggaggaa ctcaacggct tccaaaattg gttggaaagg gaatagcaaa agaattgata 480 ttttctacag atatgattac tgccgatgaa gcttatcgta taggacttgc taataaagtc 540 tatgaacctg aggaattatt agtaaaatca caggagtttg ctgaaaaggt aatgactaaa 600 tctccatggg gtgttaaatt agcaaaagca tgtataaata atggattaga tgtagatttg 660 gaagcaggac ttaaatatga agcaaattca tttggtctgt gtttttcaac ggaagatcaa 720 aaggaaggta tgaaagcatt tttagaaaaa agaaaagcag acttcaaagg actttaa 777 <210> 124 <211> 789 <212> DNA <213> Clostridium pasteurianum <400> 124 atggatttta ataatattat ccttgaaaaa gaggaaaaaa ttgccgtagt tacaattaat 60 agacctaaag ctcttaatgc tttgaacagt gaaacgttaa ctgagcttga ttctgtaatt 120 gatgaaattg acaaagataa tgaaatttta gcagtggtat taacgggagc gggaaaatcc 180 ttcgtagctg gagccgatat atcagaaatg aaagacatga atgtagtaga aggaagaaaa 240 tttggaatac taggtaataa ggtgttcaga aaacttgaaa atttagaaaa gccagtaata 300 gcagccctta atggatttac attgggtggt ggttgtgaaa ttgctatgtc ttgcgatata 360 agaatagctt ctactaaggc aaaatttgga cagccagagg tacagcttgg aataactcca 420 ggttttggcg gtactcaaag attagctaga ttaataggcc caggagctgc aaaggaactt 480 atatatactg gaaaaattat aaatgctgaa gaggcctata gattaggact tgttaataga 540 gttatagaac cagaaacttt attagatgaa gcaaaacaat tggcaaatac tatagcagcc 600 aatgcaccta tagctgttaa gttggctaaa tcagcaataa atagaggaat tcaaactgat 660 attgatacag gtgtgtcaat tgaatcagaa gtatttggag cttgtttctc tacagaagat 720 caaaaagaag gtatgaatac attcttgaat gataaaaaat atttaactgg taattttaag 780 aataaataa 789 <210> 125 <211> 783 <212> DNA <213> Megasphaera elsdenii <400> 125 atggattacc agaacattat ttttgctgta gaagacggta ttgcaacgat tacgatcaat 60 cgcccgaagg ctctgaacgc tttgaaccag gctacggtca gcgaattgaa agacgtcgtt 120 gaaaagattg cagctgataa agctatcaaa gtcgtcatca tcaccggtgc aggcgctaaa 180 tccttcgtcg ctggcgctga catcaaagaa atggcttcca agaacgctgc tgaaggccgc 240 gaatggggcc agttcggtca gaacgtcttc acggaaatcg aaaacctgcc gcagcctgtc 300 atcgcagcta tcaacggctt cgctctcggc ggcggctgcg aactctcctg cgcttgcgat 360 atccgctatg cagctgaaaa cgctaaattc ggccagccgg aagtcggctt gggcatcact 420 ccgggctttg gcggcacgca gcgcctgacc cgtgtcgtag gccgcggcca cgcgaaagaa 480 ctcatctaca cgggcggcat gatcgacgct gaaaaagcaa aagctatcgg cttggtcaat 540 gaagtcttcc cgcaggaaga actgatgccg gctgctgtta aattggctaa gaagatcgct 600 aagaacgctc ctattgcagt acagctctcc aaagctgcca tcaaccgcgg catcaactgc 660 gacgtcgtaa ccggtatcgc ttatgaagct gaagtcttcg gcctctgctt ctccacggct 720 gaccagaagg aaggcatggc tgctttctgc gaaaaacgca aagcaacgtt tgaaggtaaa 780 taa 783 <210> 126 <211> 780 <212> DNA <213> Metallosphaera sedula <400> 126 atggaatttg aaacaataga aactaaaaaa gaaggaaact tgttctggat tacgttaaat 60 agacccgata aactaaacgc actaaacgct aaattacttg aggagttaga tagggcagtc 120 tctcaggcag agtctgaccc agagattagg gttatcatca ttacagggaa aggaaaggcc 180 ttctgcgcag gggctgacat aacccagttt aaccagttaa ccccagcaga agcctggaaa 240 ttctctaaga aaggaagaga gatcatggac aagatagagg cactgagcaa acccaccatt 300 gccatgatca atggatatgc ccttgggggt ggactagagc tagccttagc ctgtgatata 360 aggatcgcag cggaggaggc ccaactaggc cttccagaga taaacctagg gatatatccg 420 gggtatgggg ggactcagag gttaaccaga gttataggaa agggaagagc cctggagatg 480 atgatgacgg gcgatcgtat tcctggtaag gatgctgaga aatatggtct cgtgaatagg 540 gttgtccccc tagctaactt ggagcaagag acaaggaagc tggcagaaaa gatagccaag 600 aagtctccta tctctctcgc cttaatcaag gaagttgtaa acaggggact agactctccc 660 ctactgtcag gtctagcgtt ggaaagcgta ggatggggag tcgtgttttc tacggaggac 720 aagaaggagg gggtaagtgc cttcctggag aagagagagc ctacgtttaa gggaaaatag 780 780 <210> 127 <211> 779 <212> DNA <213> Clostridicum kluyvery <400> 127 atggaattta aaaatatcat tcttgaaaag gatggaaatg tggcttcaat aacgttgaat 60 agacctaagg cattaaatgc attaaatgca gcaactttaa aagagataga tgccgcaata 120 aacgacattg ctgaagatga taacgtatat gctgtgataa ttactgggtc aggtaaagct 180 tttgtagcag gagcagatat agctgagatg aaagatctta ctgcagttga gggaagaaag 240 ttttcagttc ttggcaataa aatatttaga aaattagaaa atttagaaaa accagttata 300 gcagctataa atggatttgc actgggtggt ggctgtgaat tgtcattgtc ttgcgatata 360 agaatagctt catcaaaggc taagtttggt caaccagagg ttggtcttgg aattactcca 420 gggtttggag gtactcaaag acttgcaaga gcaataggcg ttggtatggc taaggaactt 480 atatataccg gaaaagtaat taatgctgaa gaggcattaa gaataggttt ggtaaataaa 540 gtagttgagc cagataaatt attggaagaa gctaaagctt tagtagatgc tattattgtt 600 aatgcaccta tagctgttag aatgtgtaag gctgctataa atcaaggact tcagtgtgat 660 atagatacag gtgtagctta tgaagcagaa gtatttgggg aatgttttgc tacagaagat 720 agagtagaag gaatgacagc atttgtagaa aaaagagaca aggcttttaa aaataagta 779 <210> 128 <211> 1509 <212> DNA <213> Sulfolobus tokodaii <400> 128 atggcaatta gaactggaga gcaatattta gattctataa aaattagaaa taaggctgaa 60 atttacgtaa tgggaaaaga agtaaaggat gtaaccactc atcccttctt gaaaccttct 120 gtaatggcat ttaaggcaac atttgatgct gcttgggaag aggacacaaa agaattagcc 180 agagcatgga gtcctttcat aaatgaagaa gtgaatagat ttaatcacat acacaggtca 240 ccagaagact tagctgctaa agtgaaatta ctgagaaaat taagccataa gaccggtgca 300 tgtttccaaa gatgtgtagg atgggacgct ctgaacactt tgtggattat gacgaatata 360 atggctcaaa aaggtaaaaa agaatataag gatagatttg tcgaatactt aagttacgtc 420 caaaagaagg atttagcatt agctggtgct atgacagatg caaaaggtgt aagaacatta 480 aaaccgcatc aacaaccaaa taagaacgct tatgttagaa ttgaggaagt taccaaagac 540 ggtatttatg tttctggtgc aaaggcaaat attactggtg tagctgcaac agaagaaatt 600 gtggttttac ctactagggc tatggggcca gaagataaag attatgctgt tgcattttca 660 ataccgacag atactgaggg tataaaaatt atagttggta gacaattaaa tgatgctaga 720 agattagaag gtggtgacat agatgcttta ccgtacttct ataaccacga gggtttagta 780 atctttgacc atgtttttgt accaatggat agagtattct taatgggaga atacgagttt 840 acttcacaat tagttgaagt attctcagca tatcatagac aaggatatgg tggttgcaag 900 gctggtttag gagatgtaat tattggtgca tcaatgaatt tagcaaaaca attaggagta 960 gaaaaagctt cacatgtaca agaaaaacta acggaaatga tattcttaac tgagaccatg 1020 tattctgcag gaattgcagc tagtttaaat gcagttaagg tctgcgataa ttgttggtgg 1080 gttaatccta tgcacgctaa tgttacaaaa catttagtag ctagatttcc agcccagatt 1140 tctcagttat ctatcgatat tgcaggtgga ataataggta ctgcaccaag tgagtgggat 1200 ctcaagaatc ctaaattaag agaatatatt gccaaatact tacaaggtgt tgagggttat 1260 acagctgaag atagattaag aatggttaga ttactggaaa acgttagtct gggtgttgca 1320 ttccaaattg aatctgtaca cggtgcagga agtccagcag cacaaagaat aatgtttagt 1380 agactttatg acttaaacta tgctgaggaa gtcgcaaaga ggttagctgg gaagaagact 1440 gatttacagt ggaaacctaa agcagagcct tggagagaaa gtgagacaga aaaattagta 1500 aaaagttaa 1509 <210> 129 <211> 1452 <212> DNA <213> Geobacter metallireducens <400> 129 atggcactaa gagatgggaa ttcctaccgg gaaagccttc gggcgctcaa tatcaaagtc 60 tatgcctttg gagagaagat tgacagcata gtagatcacc cattgttcca gccccatatc 120 aatgcggctg cattgacgtt cgacttggcc catgatccga ccacggaagc gctcgtcaca 180 gccacctcac acctgacggg gagtaaaatc agccgcttca cccatatcca ccagagcacc 240 gacgatctca taaaaaaggt gaagatgttg cggcttattg cagggaagac gggaagttgc 300 taccagcgct gtgtggggtg ggatgccctg aacgctaact atacggtaac ctatgagatg 360 gaccaggagc ttggtaccga ctatcaccag cgttttaggc gttacctcga atatatacag 420 gacaatgacc tgatggtggc gggagcaatg accgatccca agggggacag ggggctgcct 480 ccggcaaaac agaaagaccc ggacatgttc gtgcacgtgg tggcaaagaa tgacaagggg 540 atagtcattc gtggggcaaa ggttcaccag accggaattg tcaattccca tgaaatgctg 600 attatgccaa ccatggccat gggggaggag gacggcgact atgcggttgc ctgtgctctc 660 cccacggatt cccccggtgt catccatatc tttggtcgtc aaaccaacga tacacgccgt 720 ctggaaaagg gagaccttga tcagggtaat gctgagtatg gaactgtcgg aggcgaggct 780 ttgaccatac ttgaagatgt cttcgtcccg tgggaacgcg tcttcatgtg cggagagtac 840 aagtatgcgg ggctgctggt tgagcgtttc gcgagctatc atcgacagaa ctatggtgga 900 tgcaaggcag gcgtgagcga tgtgatcatc ggcgcaacta ccgctatggc agagtacaac 960 ggagcagcca aggcttccca cgtgcgtgac aagatcgtgg agatggtcca cctcaccgag 1020 accctttatt gcggttccat cgcctgctcc tgtgagggtg ctcccacgcc gtcaggggcc 1080 tatttcgtca atcccctgct ggccaatacg gttaagcaga acgtgacccg tttcatctat 1140 gagattgcac gcctttccca cgatatttcc ggtggctgca tggcaaccat gccttcggag 1200 aaggatctgc accacgatga gatcggcaaa tatgtagaga agtatttccg gggggtggac 1260 gaagctccca ctgaagagcg catgcggatg gcccggctcg ttgaaaatat gacgggcggc 1320 acggctttgg tggaaagcat gcatggtgcc ggctctcccc aggcgcagag agtcatgatc 1380 ctccgccagg caaatctcgg ccataaggta aagcttgcca agaaactggc cggcataaag 1440 gaagaaaaat ag 1452 <210> 130 <211> 1392 <212> DNA <213> Sulfolobus solfataricus <400> 130 atgagatcaa aagaagattt cctaaagtcc ttaaaagatg gaagaaattt gtattatagg 60 gggaagttag tagaagatat aacaacacat cagatcttaa agacagccgc attgcacgca 120 gctaagttat atgaatacgc tgatagagtc tatgaggata ataaaatggg aaaaatgagc 180 aagttcttta aggtaccttg gacatctcaa gatttgctag atagacataa actaatttac 240 gatttaacga tgtattgtaa tggggtattt aacatttcac aagcaatagg aagtgatgcg 300 atctttgccc ttatgatcac ggcaaaacaa gttgatagaa aatacggaac tgattactca 360 aaacgtgttg aaaaatattt tgagagagtt gctaaagaag atttaacgtt agccactgcc 420 cagactgacg ttaagggaga tcgaagtaag aggccttctg aacaagttga tccagatatg 480 tatgttagag tagttgatgt gaaaagcgat ggaatagttg ttagaggagc aaaggctcat 540 acaactcaat ctgcggtatc tgatgagatt attgtcatac caaccagagt aatgagggat 600 agcgataaag attacgcagt agcctttgcg gttccagcta atactaaagg tttgaagatg 660 tatattagac caattgatga aattgagggc aattcctcct cagtactcag tagaaaagat 720 tatgagctag aaacattaac cgtcttcaac gacgttttcg ttccttggga tagggtattt 780 ttatttaagg aatacgacta cgctggaaca ttggctatgc tatttgcaac cttccatagg 840 tttactgcat tatcgtatag gtcagcgacc atgaatctat atttgggagc atctaaagtg 900 gcatctcaag taaatggcat tgagaatgaa aagcatgtga gagatgatat agttgatata 960 attctctaca aggaaattat gaggagtagc gcgatagctg cggctgtgta tccagtaaac 1020 atggagggta tagctgtgcc caacccgctt tttactaatg ttggtaaatt atactccaat 1080 atgcatttcc atgatgttgt aagagattta attgacattg ctggggggat aatagctact 1140 atgccctctc aagaagattt ggaaagtgat gaaggaaaga atattgttaa atatttaagg 1200 ggctcagttg atggagagga aagagcaaaa gtgttaaaac tagctaagga attaggggct 1260 agtacgttta ctggctattt gctaactggt atgatacatg cggaaggttc tatggaagct 1320 agcaaaatag agctattcag aagttataat tttaaggagg ccgagaactt agttaaaagg 1380 gtattaagct ag 1392 <210> 131 <211> 1440 <212> DNA <213> Syntrophobacter fumaroxidans <400> 131 atgggactca aaacgaaggc ggaatatata gaatccttgc gaggcatgaa gccgacggtc 60 tacatgttcg gtgagaagat cgaaagcgtt gtggacaatc cacgcctgcg agcgggcatc 120 gaggcgacgg gggcgacgta cgaactggca gagacggagg agtatcgccc tctcattgtg 180 actgaaagtc ccctcattca cgaacccgtc aaccggtata cgttgccccc gtcgtccatc 240 gcggacctcg tcgccagggt gaagatcaat cgtctcatgg gcactcgtgt cgggacctgc 300 tttcaacggt gcacggggct ggactgcctg tccgcccttt ccatcgtgac ctacgacatc 360 gacgccaagc attccacccc ttacttcaaa cggttcatcg agtttctgaa gcatgttcag 420 aaaaacgacc tgacctgcaa cgccggcgtg accgacgtca agggcgaccg ttccctggcc 480 ccccacgagc aggaagacaa ggacatgtac gtgagggtcg tggaacgcaa tgcggacggc 540 atcgtcgtga ggggcgccaa ggcgcaccag accggttccc tctcctcgca cgaaatcatc 600 gtcctgccga cgcgtgccct gcgaaagggc gacgaggact acgcgctcgc ttttgccatc 660 cccaacgaca ctcccggcct gattcacgtc gtgggccgat cgagcctcga cacccgccag 720 ctggacggct gcgacctggg caaccttcac tattccaagt actgcccgac cgtgatcttc 780 aaggacgtgt tcgttccctg ggagcgggtc ttcatgtgcg gcgaggtgga attcgccgtg 840 gagatggtga accgcttttc ggcttatcac cgccagagcc acggcggctg caagtcgggc 900 aagatcgact gcatggtcgg agcggccctc accatgatgg actacaacgg gacggagaag 960 gccgggcatc tcaagcagaa ggccatcgag atggtccacc gggcggaaac cctctacggc 1020 tgcagcctgg ccgcgtccta cgagggcaaa aaagaacctt ccggaaccta cttcatcgac 1080 acggtgctgg ccaatgcgtc caagatccac gaaggcaagg aaatgagcga ggccggccgc 1140 ctgctggtgg acatcgccgg aggcttcgtg gccgatctgc cttcggatcg cgacctggcc 1200 attcccgaag tcggggaact gctgaaaaaa tacctgaagg gggtggcgtc ggtgccggtg 1260 gaagaccgcg tcaaaatgta ccggctgatc gaaaagctcg tcatggaaag cgccgatacg 1320 atttcggaca tccatggagg cggttctccc gaggcccaca ggatcacgat cctgcgggaa 1380 agcaacctca aggccaagaa ggacgcggcc aagcggttgg cgggaatcga atcgaagtag 1440 1440 <210> 132 <211> 1461 <212> DNA <213> Porphyromonas gingivalis <400> 132 atgatgacta gcgaacagta cgtagaaagt cttcggaaac ttaatctgaa ggtttacttc 60 atgggtgaaa ggatcgaaaa ccctgtagat catcccatga ttcgtccctc aatgaattca 120 gtagctatga cttataagct tgctgagatg gacgaataca agcatttaat gacagcaact 180 tcaaacttga ctggtaagca agtgaatcgt ttctgccatc tacatcagag cacagaggat 240 ctgaaagaca aagtgaagat gcagcgtctc atgggacaaa aaacagcttc atgcttccag 300 cgttgtgtgg gaatggatgc attcaatgcc atctattcta ctacttacga aatggatcaa 360 gctctgggta ccacttatca caagcgtttc atcgagtaca tgaaatatgt acaagacaac 420 gacttggtcg tagatggagc catgacagac cccaaagggg atcgcggttt atctccctca 480 gaacaagccg atccggatct ttatctgcac attgttgaag ttcgtgaaga tgggatcgtc 540 gtttccggtg caaaggcaca ccaaaccgga gcagtcaatt cgcacgagca tctgatcatg 600 cctacgatcg ctatgcgcga agctgatgct gactatgccg tttcttttgc cgttcccagt 660 gatgcagagg gcgttattat gatctatggc cgccagtcat gcgacactcg caaaatggaa 720 gaaggggcag acattgacct cggcaactct gaattcggcg gacatgaagc tcttgttgta 780 ttcgaccgcg tattcgtgcc caatgaccgc gtgttcatgt gcaaagaata ccagtttgca 840 ggtatgatgg tagaacgttt cgccggatac caccgtcagt cttatggagg atgtaaagta 900 ggtgttggtg atgtacttat cggtgcagct gctctcgcag cagactacaa tggagttcct 960 aaggcatctc acattaagga taaactcatt gagatgatcc acctgaatga aaccctttat 1020 gcttgcggta ttgcatgctc ttcagaggga actcagatga aagccggcaa ctatatgatc 1080 gatttgctgt tagctaatgt ttgtaagcaa aatatcaccc gccttcctta tgaaatagct 1140 cgcttggcag aagatattgc aggaggtttg atggtaacca tgccttctca acaagacttc 1200 cgccatccgg aaataggccc gatcgtaaag aaatatcttg caggggcaac aggcaaatcg 1260 acagaaaacc gtatgcgtgt tctgcgtttg atagagaata tcacgctggg aacagctgcc 1320 gtcggttatc gaaccgagtc tatgcacgga gccggatctc ctcaagctca gagaatcatg 1380 atcgctcgtc agggagatct tgagggcaag aaaaagcttg cacgggcgat tgctcatatc 1440 gacgaatcac tcgataagta a 1461 <210> 133 <211> 1590 <212> DNA <213> Polynucleobacter necessarius subsp. Asymbioticus <400> 133 atgagtcaaa gcacctccca gttcatgaat agcaaagact atcaagagtc attgcgctca 60 ctaaagccaa ctgtctatgt cgatggtcga ttgatcgaat ccgtcgccga tgagccttct 120 cttcgccctg gagtccaagc cttaggagtg acttatgaca tggtccatga cccagcgcta 180 gcaccgctca tgttggctga ctcgaatggc actcctgtac caagaatgct gcacattaat 240 cagtcttctg gagatctctt aaataaatta gaagcggtac gtgtactctg ccaagaaact 300 ggatgtgccc aacgctattt agcccatgat gcgttaaatg cgattgcaca agtttctgcg 360 cgcattgatg atgccaaagg aagtaatgag catagtgcta aattttctga gtatctatcg 420 catgtacaaa cgaaggactt ggcattaggc attgccatga cagatgcaaa aggagatcgc 480 tcccgcagac ctcatgagca agaaaatcca gatacttacg tacatatcgt ttctcaagat 540 gctaaagggg tcgtgatctc gggtacaaaa gcgattgtga ctggcgcccc ttacatgcat 600 gaattcttag tcatgccagg tcgcaatatg actaaagagg atgcagcctt tgcgatttgc 660 tgtgctgtcc ctgtggatgc caaaggtatt acgattgtgg cacgcccagc gggacgccca 720 ggcgacaagg tcgagcatgg taaaccgata ttttctagta aatatggtca atcgactggg 780 gtagtgatat tcgataaagt attcgttccc tgggatcgtg ttttttatgc tggcgaatgg 840 gaacactcta gcgtgctgac ttataactac gccacccatc atcgtcatag ctgcatcgcg 900 gcgcgagcag gctttggaga tctgttaatt ggtgctggcg ctttaatgtg cgaagcgaac 960 ggattggatc cagcaaccaa atctaattta cgtgatccga tggttgaact cattaagatc 1020 actgaaggat tttatgcttg cggtgtggct gctagcgtct atggaacgca agatccgtac 1080 agtaaatcat ttatgcctga gccggtattt tctaatatcg gaaaactctt attagcaacg 1140 cagatttatg acatgcatcg cttggcacat gaagtatcgg gaggattaat cgtagcgttg 1200 ccaggaccag acgaagatca caacccagca actgcagcca ctttggcaga ggtgttacga 1260 gccaatccag ccgtccctta tgacaagcga attgaagttg cacggtttat tgaagatctc 1320 acagcgtctt atcaaggcgg ttggtattcc gtcattagcc tacatggtgg cggctctcca 1380 gcagcaatga agcaagaaat ctatcgtcag taccctattg gcaataaagt agagctagtg 1440 gaacgtttat tagatcgcgg agtgctgact agtagcgaag agcgggcgat tacgaaaaat 1500 aaacaacctg ggcgctgctg cgatcaaggc tgtagcgcgc caggacaagc agtgatggta 1560 cctttgccag agcctggcag aagaacttaa 1590 <210> 134 <211> 777 <212> DNA <213> Gordonia terrae C-6 <400> 134 gtgaccgaac accagaccat cgtcgtcgag accagcggcc gggtgggcat catcaccctc 60 aaccgcccga aagcgctgaa cgcgctcaac accgagttga tgaacgaagt ggtcggcgcc 120 gtcaaggagt tcgacgtcga ccaggggatc ggcgccatcg tgatcaccgg ttcggagaag 180 gcgttcgccg cgggcgccga catcaaggag atgtcatcga agtcctacgc ggatgtggtg 240 aacgagcagt tcttcggcgc ctgggatgag ctgtcgcggg cgcgtacgcc gatcatcgcc 300 gcagtgaccg gctacgccct cggcggcggc tgcgaactcg cgatgctgtg cgacaccatc 360 atcgccggcg acaacgccgt cttcggtcag cccgagatca acctcggcgt catccccggc 420 atcggtggtt cgcagcgcct cacccgcgcc gtcggcaagg ccaaggcgat ggacatggtg 480 ctcaccggcc ggcagatgaa ggtcgacgag gccgagcgtc tgggcctggt ctcgcgggtg 540 gtgcccaagg aggactgccg cgccgccgcg atcgaagtcg ccgagataat cgcctcgaag 600 tcgctgatcg ccgccgcggc cgccaaggac gcggtcaacc gtgccttcga gtcgagcctg 660 gtggagggtg tccgcgccga gcgcgcgctg ttctactcga cgttcgcgac cgacgaccag 720 accgagggca tggccgcctt cgtcgagaag cgggacccga acttcaccca ccgctga 777 <210> 135 <211> 777 <212> DNA <213> Halalkalicoccus jeotgali <400> 135 atggcagaca gagtactcat cgaacgagag aatgacatag cgacgatcat cgttaatcgg 60 cctgagaagc gtaatgcgat ggatatcccg acgcgaaaag ccctctatgc cgccttcgaa 120 gaggttagcg aggatgacga tgtgcgggca atcgtgctcc gcggagcagg agatgggtcg 180 tttatcgccg gtggcgatat tgattctttc gccgacttcg accacatgga cggcatggag 240 tacagcgaga agtacgccca agggctgtac aactatgttg cggaccgcca caaaccaacc 300 atcgccgcgg ttgacggcta cgctctcggt ggaggcaccg aaatcgccct cgcttgcgac 360 attcgcctcg ccacggacga cgcgaagttc ggcctgcccg aagtcggcat cggcgtcatc 420 ccagccggtg gtggaacaca gcgactcgtt caagtcgtcg gagccgggct tgcaagcgaa 480 cttatcctca ctggccgcat tatcagcgcc gacgaggcaa agagaattgg tcttgcaaac 540 catgtctacg ccgccgagga attcgataat gaagtccgag ccatggccga agatcttgcc 600 tcgaaggcgc ctgtcgccca gcgacttgca aaagaatcca tccgacgtag ccttgatatc 660 gacgccggcc ttgaatacga gcgactggcc ggagcgtttc tgttcggcac cgacgaccag 720 aaagagggtg caaacgcctt ccttgaggac cgagagccga agtaccgaaa ccggtaa 777 <210> 136 <211> 774 <212> DNA <213> Carboxydothermus hydrogenoformans <400> 136 gtggaatttg aaaaaattaa atttgaggtt acggacggtt atgccgttat ttacctaaac 60 aacccgccgg taaatgctct tggccagaaa gttttaaaag atttacaaaa agctttgcag 120 gaaattgaga aaaatcccga gattcgggcg gtaataatta gcggggaagg tagcaaggtt 180 ttctgtgccg gggcagatat cacggaattt gctgaccggg ctaaagggat tttaccggaa 240 gtggaaggaa gtgttctttt ccggcaaatt gagcttttcc ccaagccggt gattgctgcg 300 ctgaacggta gctcctacgg cggaggaacc gaattagcga taagctgtca cctgcgcatt 360 ttagcagatg atgcttccat ggctttgccc gaagtaaaac tgggcattat ccctggctgg 420 ggaggtaccc agaggttacc ccggttaatt ggtaaaacca gagccctgga agcaatgctt 480 accggagagc caataacggc agaagaagcc ttaagctacg gtctggtaaa caaagtcgta 540 cccaaagacc aggtactaac agaagcccgg gcgctggcag ctaagcttgc caaaggggcg 600 cccatcgcta tgcgggaaat tttaaaggcg gtaactttag ggctggatac ttcaatagaa 660 gaaggtttaa aaattgagaa agaaggttcc aaagtggcgt ttagcagtga agatgcggtg 720 gagggaagaa ctgctttctt tgaaaaacgg ccgccgaatt ttaaaggccg gtaa 774 <210> 137 <211> 774 <212> DNA <213> Thermomicrobium roseum <400> 137 atgagcgtgc gtgtcgagcg ggagggggcg atcaccctcg tcacggtcga gcgcccggaa 60 cgactgaacg cgctcgatac cgcgacgttg cgtgccttac tcgcggcagt gcaggaactg 120 gcaacggagg aggcgatcgc tgtcgtcgtc ctcaccgggg caggcgatcg cgcgttcatc 180 gccggagccg atatcagcga gatggtagag aagtcgccag ccgaggcgct cgccttcgcc 240 gagttgggac acgccgtttg ccgggcgatc gaggaagcgc cgcaaccgta catcgcagcg 300 gtcaatggct acgcgctagg aggcggctgc gagatcgcgc tggcgtgcga tatccgcctc 360 gccagcgagc gcgccgtctt cgcccagccg gaagtaacgc tgggtattcc accaggctgg 420 ggcggatcgc aacggctgcc gcgcgtcgtt cctcctggta tcgcgcgcga gttgctctat 480 acggggcgcc gcgtcgatgc gcaggaagca ctgcggatcg ggctcgtcaa tgccgtctat 540 ccggctgacc aactcctcga gcgagctcgg gaactggcga accggatcgc ggccaacggg 600 ccactcgcgg tccgcttgac caaggcggcg gttcgcttcg gtctcgagca ggggctggaa 660 gctggactga cctacgagcg gcaggtgttc gcgtacgcgt tcaccaccga ggatcagcgg 720 gaggggatgc gggcatttct ggaaaagcgt cgtccggctt ttcgcgggcg ctga 774 <210> 138 <211> 825 <212> DNA <213> Methylobacterium extorquens <400> 138 atgaacgctg acgccgagac cgcctcgacc gacgaactgc tcttcgcggt ggatgcggcg 60 ggcatcgccc gcatcaccct caaccggccg aaggcgcgca acgcgctgac cttcgcgatg 120 tatcgcgggc tggtggagtt gtgcgagcgg atcgaggcgg accacgcgat caaggcggtg 180 atcatcaccg gcgccgggga caaggcgttc gcggcgggta ccgacatcgc ccagttccgt 240 agcttcagca aaccggaaga cgcgatcggc tacgagcgct tcatggaccg ggtgctcggc 300 ggcctggagc gcctgcgggt gccgaccatc gcggcggtcg ccggagcctg caccgggggc 360 ggtgcagcga tcgctgcggc ctgcgacatg cgcatcgcca gccgcgacgc ccgcttcggc 420 atccccatcg cccgcacgct cggcaattgc ctctcgcaga acaccctgag gcggctggcg 480 aacctcattg gggcgccccg cgtgaaggac attctgttca ccgctcggct cgtcgaggcg 540 caggaggctc tggcgatcgg cctcgtcaac gaggtggtcg aggatgccgc ggccgtcgcg 600 gcccgagcgg atgcgctggc caccctgctc gcgagccacg cgcccctcac cctccaggcc 660 accaaggaag gcctgcgccg catcggcgag gagggcgcgg cggaggccgc cgagggcgag 720 cggcccggcg acgacctgat cgtgatgacc tatatgagcg cggatttccg ggagggcatg 780 gaagccttcc tgggcaagcg cccgccgaac ttcaaagggc gctga 825 <210> 139 <211> 1224 <212> DNA <213> Clostridium sporogenes <400> 139 atgagtgata gaaataagga agtaaaagaa aaaaaggcaa agcattatct tagagagatt 60 actgcaaagc attacaaaga agctctcgaa gcaaaagaaa ggggagaaaa ggttggttgg 120 tgtgcatcta acttcccaca agaaatagct acaacattgg gggtaaaagt tgtttatcca 180 gaaaatcatg cagcagctgt agcagctaga gggaatggac aaaatatgtg tgaacatgct 240 gaggctatgg gtttttctaa tgatgtatgt ggttatgcaa gagtaaattt agctgttatg 300 gacataggtc atagtgaaga tcaaccaata cctatgccag actttgtact ttgctgtaat 360 aacatttgta atcaaatgat taaatggtat gagcatatag caaaaacttt agatatacca 420 atgattctta tagatatacc atacaataca gaaaatactg tttcacaaga tagaattaaa 480 tatattagag cacaatttga tgatgcaata aaacaattgg aagaaataac aggcaaaaaa 540 tgggatgaaa ataaatttga agaagttatg aaaatatccc aagaaagtgc aaaacaatgg 600 ttaagagcag catcctatgc aaagtataaa ccttcaccat ttagcggatt tgatttattt 660 aatcatatgg ctgtagcagt ttgtgcaaga ggtacacaag aagctgcaga tgcatttaag 720 atgttagcag atgaatatga ggagaatgta aaaactggaa aatccactta taggggagaa 780 gaaaaacaac gtatattatt tgaagggatt gcctgttggc catatttgag acataaatta 840 actaagctta gtgaatatgg tatgaacgta actgcaactg tatacgcaga agcctttggt 900 gttatatatg agaatatgga tgaattaatg gctgcttata ataaagttcc taattcaatt 960 agttttgaaa acgcattaaa aatgagatta aatgctgtta caagcactaa tacagaaggt 1020 gctgttattc atataaatag aagctgtaaa ttatggagtg gatttttata tgagctagca 1080 agaagattag aaaaggaaac aggaattcct gtagtatcat ttgatgggga ccaggcagac 1140 ccaagaaatt tctcagaagc tcaatatgat actagaattc aaggacttaa tgaagtaatg 1200 gttgctaaaa aggaggctga ataa 1224 <210> 140 <211> 1125 <212> DNA <213> Clostridium sporogenes <400> 140 atgtcaaatt cagataaatt ttttaatgac tttaaggata ttgtagaaaa tcctaaaaaa 60 tatataatga agcatatgga acaaactgga caaaaggcta taggatgtat gccattatat 120 actcctgagg aacttgtatt agctgctgga atgtttccag taggggtatg gggaagcaat 180 acagaacttt caaaagctaa aacatatttc ccagcattta tttgttcaat attacaaaca 240 acattggaaa atgcattaaa tggagaatat gatatgttat ctggtatgat gattacaaat 300 tattgtgatt cattaaaatg catgggacaa aattttaaac taaccgttga aaatattgag 360 tttatcccag taacagttcc acaaaataga aaaatggaag ctggaaaaga gtttttaaaa 420 agtcaatata aaatgaatat tgagcaatta gaaaagattt ctggtaataa aataacagat 480 gaatctttag aaaaagctat agaaatatat gatgaacaca gaaaagtaat gaatgacttt 540 tcaatgttag catcaaaata tccaggtata ataacaccaa ctaaacgtaa ttatgttatg 600 aaatctgctt attatatgga taaaaaagaa catactgaaa aagttagaca attaatggat 660 gaaattaaag ctatagaacc aaaaccattt gaaggaaaga gagttataac tacaggtata 720 attgcagatt cagaagattt acttaaaata ttagaagaaa ataatatagc tatagttggt 780 gatgatatag cacatgaatc tagacaatat agaacattga ctccagaagc gaacacacca 840 atggataggt tagctgagca atttgctaat agagaatgta gtactttata tgatcctgaa 900 aagaaaaggg gtcaatatat agtagaaatg gctaaagaga gaaaagcaga tggaattata 960 tttttcatga caaaattctg tgacccagag gaatatgatt atccacaaat gaaaaaggat 1020 tttgaagaag caggcattcc acatgtacta atagaaactg atatgcaaat gaaaaattat 1080 gaacaagcta gaactgcaat tcaggctttt tcagaaacac tttaa 1125 <210> 141 <211> 795 <212> DNA <213> Clostridium sporogenes <400> 141 atggcagaca tttatactat gggtgtagac ataggttcaa ctgcatcaaa aacagtagta 60 ttaaaaaatg gtaaagaaat tgtaagtcaa gcagtaataa gtgtaggggc cggaacaagt 120 ggccccaaga gagctataga ttctgtatta aaagatgcta aattatccat tgaagattta 180 gactatattg tatccactgg atatggaaga aatagtttcg attttgctaa caaacaaatt 240 tctgaattaa gttgtcatgc aaaaggggtc tatttcgata acaataaagc tagaacagtt 300 attgatatag gcggacaaga tattaaagta ttaaaattag cggatagtgg aagactttta 360 aactttataa tgaatgataa atgtgctgca ggaacgggac gatttttaga tgtaatgtct 420 agagtaatag aagttccagt tgatgagtta ggaaaaaaag cattagaaag caaaaatcct 480 tgtactatta gttctacctg tacagtattt gcagagtcag aagtaatttc tcaacttgca 540 agaggagtta aaactgaaga tttgatagca ggaatttgta aatctgtagc atcaagagtg 600 gctagccttg caaagagaag tggtatagaa gaattagtag ttatgagtgg aggagtagct 660 aaaaatatag gtgtagtaaa ggcaatggaa gcagaattgg gaagagacat atatatatct 720 aaaaattctc aattaaatgg agcattggga gcaagtctat acgcttatga aagttttcaa 780 aaagaaagga gctaa 795 <210> 142 <211> 1239 <212> DNA <213> Clostridium sporogenes <400> 142 atggaaaaca atacaaatat gtttagtgga gtaaaggtta ttgaattagc aaattttata 60 gctgctccag cagcaggtag attttttgct gatggtggtg cagaggtaat aaaaattgaa 120 tcacctgctg gagatccttt aagatatact gctccttcag aaggaagacc attaagccaa 180 gaagaaaata ctacttatga tttggaaaat gcaaataaaa aagcaatagt attaaatctt 240 aaaagcgaaa aaggtaaaaa gatattacat gaaatgttag cagaagcaga tatattatta 300 actaattgga gaacaaaggc tttagttaaa caaggattag actatgaaac actaaaagaa 360 aaatatccta aattagtttt tgcacaaata actggttatg gtgaaaaagg accagataaa 420 gatcttccag gctttgatta tactgcattt ttcgctagag gcggtgtttc aggtactctt 480 tatgaaaaag gaactgtgcc tccaaatgtt gttccaggac ttggagacca tcaagctggg 540 atgtttttag cagcgggtat ggcaggagct ttatataaag caaaaacaac aggacaagga 600 gataaagtaa cagtaagttt aatgcatagt gctatgtatg gactaggtat tatgatacaa 660 gctgctcaat ataaagatca tggattagta tatccgataa atcgtaatga aactccaaat 720 ccttttatag tttcatataa atctaaggat gattactttg ttcaagtatg tatgccacca 780 tatgatgttt tctatgatag atttatgacc gctttaggaa gagaagattt agttggagac 840 gaaagataca ataaaataga aaatttaaaa gatggacgtg ctaaggaagt atacagtata 900 atcgaacaac aaatggttac aaagacaaag gatgaatggg ataacatatt tagagatgca 960 gacattccat ttgctatcgc acaaacttgg gaagatttat tagaagatga acaagcttgg 1020 gcaaatgatt atttgtataa gatgaaatat ccaacaggaa acgaaagagc attagtaaga 1080 cttccagtat tctttaaaga agcaggatta ccagaatata atcaatcacc acaaatagca 1140 gaaaatactg tagaagtttt aaaagaaatg ggatatacag aacaagagat tgaggaatta 1200 gaaaaagata aagatataat ggtaaggaag gaaaaataa 1239 <210> 143 <211> 1107 <212> DNA <213> Lachnoanaerobaculum saburreum <400> 143 atgtggcatt gtttagaaac tttaaaaaag attagtgcgt ctccaaagga acagcttaat 60 aaataccttg aagaaggaaa aaaagttatt gctgttgcac cggtttatac acctgaggag 120 attatccatg cttttggatt tgtacctatg ggggtatggg gcgcagatat tgaaattaat 180 gagtcaaaaa aatattatcc tgcatttatt tgctcaataa tgcagacagt attggagctg 240 ggaataaagg gaaattataa cggagttagt gctatagtgg ttccttcgct atgtgactca 300 ttaaaaactt tgggacaaaa ttggaaatat gcggtaaagg acattccttt tataccaatg 360 acctatccac aaaatagaaa atctgattat gctgttgatt tcacattgga gatgtataag 420 agagtgatca gtgatttgga aaatattacc ggagaaaagt ttgatgaagg taaactcaaa 480 aacacttatg aaatttataa tgagcataat agggttatga gagaatttac aaaagtttcg 540 gaagagtatg aagtttcggc aacagataga agtgcagtat ttaaaagtgc ttggtttatg 600 cttaaggagg aacatacaga acttgttagg gaattgatcg aacttataaa aaaagagggt 660 aaaatatcta agaagctaag aatttataca acaggaatat tggcggatgc accggattta 720 ctcaatattt ttgacagcaa taatatgcaa atcgtaggtg atgatattgc ttatgaatcc 780 agacagtata gaacagatat acccgatgga aatggtttat atgctcttgc aaagaagttt 840 tcaaatatgg acaactgtac tcttttatat gataaggata agagaagggt tgactttatt 900 attgaagaag caaagaaaaa aagagctgac ggaatagtag ttcttatgac caagttttgc 960 gatcctgaag aatttgacta tgtgcctata aagagggcgg caaatgaagc aggtattcca 1020 catatcaata tagaagtgga tagacaaatg aaaaattatc aacaggcaaa tactatgtta 1080 caaacatttg cagacatgtt ggtttag 1107 <210> 144 <211> 1230 <212> DNA <213> Lachnoanaerobaculum saburreum <400> 144 gtggaagaag ctaaaaaaca aaagcctaca gttgatccaa acagcgcaaa ggctagattg 60 ggcaggatag cagcaaaagc atatagtgac tgtgttgagg ctaaaaagcg aggagaattg 120 gtaggatggt gtgcaagtaa ttttccggtg gagatacctg agaccttggg attgtacgta 180 tgttaccctg agaatcaggc ggcaggtatt gctgccagag gcggtggaga acgaatgtgc 240 agtgagagtg aaggtgacgg atactctaat gatatatgcg catatgcaag aatttcgctt 300 gcatatatga agctgaagga agctcctgaa caggatatgc cacagcctga ctttgttcta 360 tgttgtaata atatatgcaa ctgcatgatt aagtggtatg aaaatatagc aaaagaactt 420 aatattccta tgattatgat tgatatacct tttaatcctg attatgaagt ttcagatgct 480 atgacagcat atatcagaaa tcagttttgg gatgcaatac atcaattgga ggaaattaca 540 ggcaaaaaat ggagtaatga aagatatgaa gaggtaagga aaatatcagg aagaagctcc 600 agagcatggc ttgaggctac agcgactgcc aaatattcac catctccgtt taacggattt 660 gatttattaa atcatatggc ggttatggtt actgccagag gaaaacttga agctgcagaa 720 gcaatggaaa cacttttgca ggagtacaag gataatcatg agaagggaga gtctacgttc 780 aagggagaag aaaaatatag aataatgttt gagggtatag catgctggcc atggcttcgt 840 gctactgcta caggacttaa gagtcgtgga atcaatatgg ttacaactat atatgcggat 900 gctttcggat ttatctatga tgactttgac ggaatgtgca gagcatatgc caatgttcct 960 aattgtatga atatagagca tgcaagagat aagagaataa aactttgtaa ggacaatagt 1020 gttgaagggc ttctcgttca cacaaacagg tcttgtaaac tttggtcagg atttatgtct 1080 gaaatgagca ggcaaatagg tgaagaatgt ggtattccgg ttgtaagctt tgatggagac 1140 caagcagatc caagaaattt ctcagaggct caatatgata cgagagttca gggattgaca 1200 gagataatgg aagcaaataa ggaaatttaa 1230 <210> 145 <211> 771 <212> DNA <213> Lachnoanaerobaculum saburreum <400> 145 atgtacacat tgggtgttga tataggctca actacatcca aagcggtaat attggaggat 60 ggagaaaata tagttgcatc ttcaattgtt atagcaactg taggaacggc aggagtagaa 120 gaggctgtaa aaaatgtact aaacttttca aaactcgaac taaatgacat taaagcagtg 180 gttgctacag gatatggaag aatgaattat gatgtagcag attacaaggt tagtgaattg 240 acatgtcatg cattaggtgt acataaggag ttcccgaatg tcagaactgt aattgatatc 300 ggaggtcagg atgccaaggt aatatctctt gcggcaaacg gtaagatgac aaattttgtt 360 atgaatgata aatgtgcggc agggacaggt agatttcttg atgtaatggc taatatatta 420 aatcttgata tacaggattt ggaggtggaa gccttaaaat cagataatcc ggcaaatata 480 tcaagtactt gtacagtttt tgcggaatcg gaagtcatat cacagcttgc tacaggaaga 540 aatattcctg atttggttgc agggatatgc aaatctgttg cagtaagggt tgccgccctg 600 gctaaacgag taggtatagt tgaagaagtg tgtatgagcg gcggagtggc aaaaaactcg 660 ggtgtgagga atgctatgag taaagagctt ggtgtagata tagtgtttag taaggatgct 720 caacttatgg gagcacttgg agccgcaata tacggtttta aaaagttata a 771 <210> 146 <211> 795 <212> DNA <213> Peptostreptococcus stomatis <400> 146 atgagcagtg tatacacaat gggtattgac attggatcaa catcatcaaa gtgtgtgata 60 atgaaggatg gtaaggaaat tgtaagtgaa ggtgtagtta gcttgggtgc tggaactaag 120 ggttctgacc tagttattga ggaagtgctt ggtaaggcag gaatgacttt cgatgaaata 180 gacctaatcg tatcgactgg atatggtaga aatagctatg aaagagctgc caagactgtt 240 agtgagctta gttgtcatgc caagggtggt ggatatatct ttggtggtgc cggaactatt 300 atagatatcg gtggtcagga tataaaggta ttgaagctaa atgacaaggg tggtcttgtt 360 aacttcctga tgaatgataa gtgtgctgcc ggtacaggta ggttcttgga agttatgtct 420 ggcgtattgg atgtaaagct agatgaacta ggggaactag atgccaaggc tacagaagtt 480 acaccaatca gttctacatg tacagttttt gctgagtcag aagttatatc atgtatggct 540 aagaagattc ctctagaaaa tatcataaga ggtatacacg catctgttgc aacaagggtt 600 gctagtttgg caagaagagg tggtttgaag actcctgtag ccatgacagg tggagttagt 660 aagaacaagg gtatagtaag ggctcttaaa gaagaactag aatgtgatat cttgatatct 720 cctgattctc agatggctgg tgctataggt gcagccctat atgcatatga cgaataccag 780 aagcaaaacg cttaa 795 <210> 147 <211> 1119 <212> DNA <213> Peptostreptococcus stomatis <400> 147 atgagtaata tagatgtatt gttaggtaaa cttgatgtaa gtcttttggg acaggtagac 60 aagtatgttt cagaaggtaa gaaggtaata ggttgcgcgc cagtttatac accagaagaa 120 ttagtatatg ctgcaggcat ggtaccaatt ggtgtatggg gtgcagaagg tgaagtaggt 180 ctatcaaagg aatacttccc agcattttat gcagctataa tccttagatt aatggacctt 240 ggtttagaag gtaagcttga caagatgtca ggtatgatta taccgggact aagtgacggt 300 ctaaagggac ttagccagaa ctggaagagg gctataaagc aggttccggc cctatacata 360 ggctatggtc agaacagaaa aattgaagct ggtattactt acaatgaaaa gcagtacatc 420 aagctaagag gacagttaga agaaatagct ggttgcaaga tagaagatgc taaggttgaa 480 gaggctatag ttctttacaa caagcacaga aaggcaatgc aggaattcag ttctctagca 540 gctagtcact taaatactat tacacctatt ctaagagcta gagtaatgac aagtgccttc 600 ttgttcgaca aggcagaaca tttagctata ttggaagaat tgaataaaga attaaaggcg 660 ttacctgaag aaaaatttgc tggcaagaag gtagttacta ctggtattct tgcaaatagc 720 ccaggtatgc tagaaatact agatgagtac aaacttggta tagttgatga caatatcaac 780 catgaatcag gccagtttga ctacctagtt gatgaaggta ctggtaatcc agttagagcc 840 ttatctaagt ggatttcaga tatagaagga agtactttgt tgtatgatcc agaaaaactt 900 aggggacaga taataattga caaggttaag aagcatcagg cagatggtgt tatataccta 960 atgactaagt ttagtgattc tgatgaattc gactatccaa tcatcagaaa agaattagaa 1020 aatgcaggta tcttgcatat actagttgag gttgatcagc aaatgactaa ctttgaacag 1080 gcgaaaacag cattacagac tttcgctgat atgatttaa 1119 <210> 148 <211> 1236 <212> DNA <213> Peptostreptococcus stomatis <400> 148 atgagtaata caggaatggt agaagaaaag ccggcaaaag tattgttagg agaaattgtt 60 gcaaagcact ataaggaagc ttgggaggca aagaataatg gtgaactagt tggatggtgt 120 gcatctaact tcccacagga aatattcgaa actatggata taaaggttgt ttatccagaa 180 aaccaggctg ctgctatatc tgctaagggt ggcggacaga gaatgtgcga aatagctgaa 240 aatgaaggat attcaaatga tatctgtgct tacgctagaa tatctttggc atacatggac 300 gttaaggatg ctccagaatt aaatatgcca cagccagact tcgttgcttg ctgtaacaat 360 atttgtaact gtatgatcaa gtggtatgaa aatatagcta aggaattgaa tataccttta 420 attttaatag acgttcctta caacaatgac tacgaggctg aagacgatag agttgaatat 480 ctaagaggtc agtttgatta tgctatcaag cagttagaag aactaactgg caagaagtgg 540 gatgaaaaga agtttgaaga agtaatggaa gtttctcaga gaacaggtag ggcttggtta 600 aaggctactg gatatgctaa gtatactcca tcaccattct caggctttga cgtattcaac 660 cacatggctg ttgcagtttg tgcaagaggt aagatagaat cagctatagc attcgaaaag 720 ctagctgaag aatttgacga aaacgtaaga actggtaagt caacatttaa gggcgaagaa 780 aagttcaggg tgttatttga aggtatagca tgttggccac acctaagaca tacattcaag 840 cagcttaagg atgctggtgt taatgtctgt ggtacagtat atgcggatgc tttcggatat 900 atctatgaca atacatatca gttaatgcag gcttactgcg gaactccaaa tgctatttca 960 tacgaaaggg caactgatat gagactaaag gttattgaag aaaacaatat agatggtatg 1020 ttaatccaca tcaacagaag ttgtaagcag tggtcaggta tcatgtacga gatggaaaga 1080 gatattagag aaaagactgg tataccaaca gctacattcg atggtgacca ggccgatcca 1140 agaaacttct ctgaagctca gtatgatact agagtacagg gtcttataga actaatggaa 1200 gctaataaag ctgcaaagat gaaggaggcg cactaa 1236 <210> 149 <211> 1227 <212> DNA <213> Clostridium difficile <400> 149 atgtctgaaa aaaaagaagc tagagtagta attaatgatt tattagctga acaatatgca 60 aatgcattta aagctaaaga agaaggaaga cctgtaggtt ggtcaacatc agtatttcct 120 caagagttag cagaagtatt tgacttaaac gtattatatc cagaaaacca agcagctgga 180 gtagcagcta aaaaaggttc tttagaatta tgtgaaatag ctgaatctaa aggatattct 240 attgacctat gtgcatatgc aagaacaaat tttggtcttt tagaaaatgg tggatgtgaa 300 gctttggata tgccagctcc agatttccta ctttgctgta acaatatatg taaccaagtt 360 ataaaatggt atgaaaatat ttcaagagaa ttagatatac ctttaataat gattgataca 420 actttcaata atgaagacga agttactcaa tcaagaatag attatattaa agctcaattt 480 gaagaagcta taaaacaact agaaattata tcaggaaaga aatttgaccc taagaagttt 540 gaagaagtaa tgaaaatatc agctgaaaac ggaagactat ggaagtattc tatgagttta 600 ccagcagatt cttctccttc tccaatgaat ggatttgact tatttactta catggctgta 660 atagtttgtg ctagaggtaa aaaagaaact acagaagcat ttaagttact tatagaagaa 720 ttagaggaca acatgaaaac tggtaaatct tctttcagag gggaagaaaa atacagaata 780 atgatggaag gtataccttg ttggccatat ataggataca agatgaaaac attagctaaa 840 tttggagtta acatgacagg tagtgtttac ccacatgctt gggcattaca atatgaagtt 900 aatgatttag atggaatggc agtagcatat agtactatgt ttaacaatgt aaacctagac 960 cgtatgacaa aatatagagt tgattcttta gtagagggta aatgtgatgg agcattctat 1020 catatgaaca gaagctgtaa acttatgagt ttaatacaat atgaaatgca aagaagagca 1080 gctgaagaaa ctggattacc atatgctgga tttgatggtg accaagcaga ccctagagct 1140 ttcactaatg ctcaatttga aacaagaatt caaggtttag ttgaagtaat ggaagaaaga 1200 aaaaaactta atagaggtga gatataa 1227 <210> 150 <211> 1125 <212> DNA <213> Clostridium difficile <400> 150 atggaagcta ttttatctaa aatgaaagaa gtagttgaaa atccaaatgc ggctgtaaaa 60 aaatataaaa gtgaaactgg taaaaaagct ataggttgtt tcccagttta ttgcccagaa 120 gaaattatac atgcagctgg aatgcttcca gttggtatat ggggaggaca aacagaatta 180 gatttagcta aacaatattt ccctgcattt gcatgttcaa taatgcaatc atgtttagaa 240 tatggattaa aaggtgctta tgatgaatta tctggagtta ttataccagg tatgtgtgat 300 acactaattt gtttaggaca aaactggaaa tcagcagtac ctcatataaa atatatatca 360 ttagtacacc cacaaaatag aaaacttgaa gctggtgtaa aatacttaat cagtgagtac 420 aaaggcgtaa aaagagaact tgaagaaatt tgtggatatg aaatagaaga agcaaaaatt 480 catgaaagta tagaagttta caatgaacat agaaaaacta tgagagactt tgttgaagta 540 gcttataaac attctaatac tataaaacca tcaataagaa gcttagtaat taagagtggg 600 ttctttatga gaaaagaaga acatactgag ctagtgaaag atttaatagc aaaattaaat 660 gctatgccag aagaagtctg ttctggaaag aaagttttat taacaggtat attagctgat 720 tctaaagata tattagacat tttagaagac aacaatatat cagttgtagc tgacgactta 780 gcacaagaaa caagacaatt cagaacagat gtaccagcag gtgatgatgc gttagagaga 840 ttagcaagac aatggtcaaa catagaagga tgttcattag cttatgaccc taagaaaaaa 900 cgtgggtcac ttatagtaga tgaagttaaa aagaaagata tagatggtgt tatcttctgt 960 atgatgaaat tctgtgaccc agaagaatac gattatcctt tagttagaaa agatatagaa 1020 gatagtggaa tacctacttt atatgttgaa atcgaccaac aaactcagaa taatgaacaa 1080 gccagaactc gtattcaaac ttttgctgag atgatgagtt tagcg 1125 <210> 151 <211> 798 <212> DNA <213> Clostridium difficile <400> 151 atgtacacaa tgggattaga tataggttca actgcatcaa agggagtaat cttaaagaat 60 ggggaagata ttgtagcttc tgaaacaata tcctctggta ctgggactac tggaccatca 120 agagttttag aaaaattata tggcaagaca ggtcttgcaa gagaagatat taaaaaagtt 180 gtagttacag gatatggaag aatgaactat tcagatgctg ataagcaaat aagtgaatta 240 agctgtcatg ctagaggggt aaatttcata attccagaga caagaaccat tattgacata 300 ggtggtcaag atgcaaaggt attaaaatta gataataatg gaagactatt aaactttctt 360 atgaatgaca aatgtgctgc aggtacagga agatttttag atgtaatggc aaaaataata 420 gaggttgatg tatctgaact cggaagtata tctatgaatt ctcaaaatga agtatcaata 480 agcagtacat gtacagtatt tgcagagtct gaggttatat cacatttatc tgaaaatgca 540 aaaattgaag atatagtggc aggtattcat acttcagtag caaagagagt ttctagccta 600 gtaaaaagaa taggagtaca aagaaatgta gttatggttg gtggggttgc tagaaatagt 660 ggtattgtaa gagctatggc aagagaaatc aacacagaaa ttattgtacc tgatatacct 720 caattaactg gtgctttagg agcagcgtta tatgcttttg atgaagcaaa agaatcacaa 780 aaagaagtga aaaatata 798 <210> 152 <211> 1194 <212> DNA <213> Clostridium difficile <400> 152 cttttagaag gagttaaagt agtagaactt tcaagtttca tcgcagcacc atgttgtgca 60 aaaatgttag gtgactgggg tgcagaggtt attaagattg aacctataga aggtgatgga 120 ataagagtta tgggtggaac atttaaatct ccagcatcag atgatgaaaa ccctatgttt 180 gaattagaaa atggaaataa aaagggtgta agtattaatg taaaatcaaa agaaggagta 240 gaaatattac ataaattatt atcagaagca gacatatttg taactaatgt tagagttcaa 300 gcattagaaa aaatgggtat agcttatgac caaataaaag ataagtatcc aggattaata 360 ttctctcaaa tattaggata tggtgaaaaa ggacctttaa aagataaacc aggatttgac 420 tatactgcat acttcgcaag aggaggagtt agccaatctg ttatggaaaa aggaacatct 480 ccagcaaata cagcagcagg atttggtgac cactatgcag gtctagcact agcagcagga 540 agtttagcag cattacataa aaaagctcaa actggtaaag gtgagagagt aacagtaagt 600 cttttccata cagctatata tggaatggga acaatgataa caacagcaca atacggaaat 660 gaaatgcctt tatcaagaga aaatccaaac agcccattaa tgactacata taaatgtaaa 720 gatggaagat ggattcaatt agctttaata caatacaaca agtggttagg caaattctgt 780 aaggttataa atagagaata tatattagaa gacgatagat ataataacat agattcaatg 840 gttaatcatg ttgaagattt agttaagata gttggagaag ctatgttaga aaaaacatta 900 gacgagtggt cagctttatt agaagaagca gacttaccat ttgaaaaaat tcaaagctgt 960 gaagatttat tagatgacga acaagcttgg gcaaatgact tcttatttaa gaaaacatac 1020 gatagcggaa atacaggtgt cttagttaat actccagtta tgtttagaaa tgaaggaatt 1080 aaagaatata caccagcacc aaaagtaggt caacatactg tagaagtatt aaaatcttta 1140 ggctacgatg aagagaaaat aaataacttt aaagatagta aagttgtaag atat 1194 <210> 153 <211> 768 <212> DNA <213> Escherichia coli (strain K12) <400> 153 atgagcgaac tgatcgtcag ccgtcagcaa caagtattgt tgctgaccct taaccgtccc 60 gccgcacgta atgcgctaaa taatgccctt ctgacgcaac tggtaaatga actggaagct 120 gcggctaccg atagcagcat ttcggtctgt gtgattaccg gtaatgcacg cttttttgcc 180 gctggggccg atctcaacga aatggcagaa aaagatctcg cggccacctt aaacgataca 240 cgcccgcagc tatgggcgcg attgcaggcc ttcaacaaac ctctcatcgc agccgtcaac 300 ggttacgcgc ttggtgcggg ttgcgaactg gcattgttgt gcgatgtggt ggttgccgga 360 gagaacgcgc gttttggttt gccggaaatc actctcggca tcatgccagg cgcaggagga 420 acgcaacgtt taatccgtag tgtcggtaaa tcgttagcca gcaaaatggt gctgagcgga 480 gaaagtatca ccgctcagca agcacagcag gccgggctgg ttagcgacgt cttccccagc 540 gatttaaccc tcgaatacgc cttacagctg gcatcgaaaa tggcacgtca ctcgccgctg 600 gccttacaag cggcaaagca agcgctgcgc cagtcgcagg aagtggcttt gcaagccgga 660 cttgcccagg agcgacagtt attcaccttg ctggcggcaa cagaagatcg tcatgaaggc 720 atctccgctt tcttacaaaa acgcacgccc gactttaaag gacgctaa 768 <210> 154 <211> 774 <212> DNA <213> Rhodobacter capsulatus <400> 154 atgagctatc acacgatccg ctacgagatc tccgaagggc tggcggtgat cacgctcgat 60 cgccccgagg tgatgaatgc gctgaacgcg gcgatgcggc acgaattgac cgcggcgctg 120 caccgcgcgc ggggcgaggc gcgggcgatc gtgctgaccg gatcggggcg ggccttttgc 180 tctgggcagg atctgggcga tggcgcggcc gaggggctga acctggaaac cgtgctgcgc 240 gaggaatacg agccgctttt gcaggcgatt tacagctgtc cgctgccggt tctggcggcg 300 gtgaacggcg cggcggcggg ggcgggggcc aatctggctc tggcggccga tgtggtgatc 360 gcggcgcaat ctgcggcctt catgcaggct ttcacccgga tcgggctgat gccggatgcg 420 ggcgggacct ggtggctgcc gcggcaggtc ggcatggccc gcgccatggg gatggccctg 480 ttcgccgaga agatcggcgc cgaagaggcc gcgcgcatgg ggctgatctg ggaagccgtg 540 cccgatgtcg atttcgagca tcactggcgg gcccgggcgg cgcatctggc gcggggccct 600 tcggcggcct ttgcggcggt gaagaaggcc tttcatgccg gtctgagcaa tcccctgccc 660 gcgcagctgg cgctggaagc ccggttgcag ggcgaactgg gccagagcgc ggatttccgc 720 gagggcgtgc aggcctttct ggaaaagcgc ccgccgcatt tcaccgggcg ctag 774 <210> 155 <211> 2106 <212> DNA <213> Pseudomonas stutzeri <400> 155 atgacggatg tcattcggct cgaacgccgg ggcgatatcg ctctgatcct ggtcaacaac 60 ccgccggtca acgcccttgg ccatgccgta cgaaaaggcc tgttggatgc ctttcaagag 120 gctgacgagg cgcccgaggt gacggccgtg gtgctggtct gcgaaggccc gaccttcatg 180 gccggcgccg atatcaagga gttcggcaaa ccgccgcagg caccgagcct gccggaggtg 240 atcgaggtga tcgagggctg ccgcaagccg agcgtcgcgg tgatccacgg caccgccctg 300 ggtggtgggc tggaggtcgc gctgggctgc cattaccgta tcgcccggtc ggacgccaag 360 gtcggcctgc cggaggtgaa gctgggcctg ctgcccggcg ccggcggtac ccagcgcttg 420 ccgcggctgg ccggtgtcga gaaggcgctg gagatgatcg tcagcggcca gcccatcggt 480 gcggcggagg cgctggagca ctatatcgtc gacgagctgt tcgaaggcga tctgatcgag 540 gccggtctga cctatgcgcg tcgccttgtc gaggagggcc gcggtccgcg ccgcagtggc 600 gagcagaccc gcggtctgga aggcgtcgac aacgaggcgc tgattcgcgc caagcacgcc 660 gaggtggcca agcgcatgcc ggggctgttc tcgccgctgc gctgcattgc cgcggtggaa 720 gccgccacca ggctgccgct ggccgaaggc ctcaagcgcg agcgcgagtt gttcaccgag 780 tgcctgaatt caccgcagcg cggcgcgctg atccattcgt tcttcgccga gcgtcaggcc 840 ggcaagatcg acgacctacc atccgacgtc accccccgcc cgatcaggac cgccgcggtg 900 atcggcggcg gcaccatggg cgtcggcatc gccttgagct tcgccaacgc cggggtgccg 960 gtgaagctgc tggaaatcaa tgacgaggcg ttgcaacgcg gcctgcagcg tgcccgcgaa 1020 acctacgcgg cgagcgtcaa gcgcggcagc ctgaccgagg atgcgatgga gcagcgcctc 1080 gcgctgatcg ctggcgtcac cgactacggc gccctggctg atgccgacgt ggtggtcgag 1140 gccgtgttcg aagagatggg cgtcaagcag caggtcttcg agcaactgga tgcggtgtgc 1200 aagccgggtg cgatcctcgc ctccaacacc tcgtcgctgg acctgaacgc catcgccggc 1260 ttcaccaggc gccccgagga tgtggtcggc atgcacttct tcagcccggc caatgtcatg 1320 cgcctgctgg aagtggtgcg cggtgagcgg accagcgatg aagtgctcgc cgccgccatg 1380 gcgatcggca agcagctgaa gaaggtctcg gtggtggtcg gcgtctgcga cggcttcgtc 1440 ggcaaccgca tggtcttcca gtacggccgc gaggcggagt tcctgctgga ggaaggcgcc 1500 acgccacaac aggtcgacgc tgccctgcgc aatttcggca tggccatggg accgttcgcc 1560 atgcgcgatc tgtccggtct cgacatcggc caggcgatcc gcaagcgcca gcgcgcgacg 1620 ctgccggcgc acctggattt tcccaccgtc tcggacaagc tctgcgccgc cggcatgctg 1680 gggcagaaga ccggtgccgg ctactaccgc tacgaacccg gcaaccgcac cccgcaggag 1740 aatcccgacc tcgcgcccat gctggaagcc gcgtcgcggg aaaagggcat cgagcggcag 1800 gcgctggacg agcagtacat cgtcgagcgc tgcatcttcg cgctggtcaa cgagggcgcg 1860 aagattctcg aggaaggcat tgcccagcgc tccagcgaca tcgacgtcat ctacctcaac 1920 ggctacggct tcccggcctt ccgcggcggg ccgatgtact acgccgacag cgtcggcctg 1980 gacaaggtgc tggcgcgagt aaaagaactg cacgcgcgtt gcggcgactg gtggaagccg 2040 gcgccactgc tggaaaaact ggccgccgaa ggccgcacct tcaccgaatg gcaggccggg 2100 caatga 2106 <210> 156 <211> 1968 <212> DNA <213> Haliangium ochraceum <400> 156 atgatcgtcg gagtcatcgg gtcgggcgcc atcggcccag acctcgccta cggattcgcc 60 tcggccctgg ccagcgttcc cggcgccagg gtctatctac acgatatcaa gcaggaggcc 120 ctcgacgccg gtatgcagcg catccgcggc tacatcgcca agggcctggc ccgcggcaag 180 atcagcgaac gcgtcgccgg cgccctggag acggtgctcg tgcccacgct ctcgctcgcc 240 gatctcgcgc cgtgcagcta cgtgctcgag gccgccaccg aggagctcgg ggtcaagcgc 300 gccatcttgc gcagcctcga ggatacagtc gatagcgagt gcctcatcgg cttcgccacc 360 tcgggcctgc cgcgcgcgat catcgccgcc gaggtcaaac atcccgagcg ctgcttcgtc 420 aatcacccct tctaccccgc ctggcgttcg ctgcccgtcg aggtcgtgct ctcgggtagc 480 ccggcgcacg gccagcgcat gctggccacc ctcgaggccc tgggcaaagt ccccgtcatc 540 accgcggacg cgccctgctt cgcggccgac gacatctttt gcaactactg ctcggaggcc 600 gcgcgcatcg tcgaggaagg catcgccaat cccgcccagg tcgacgccat cgtccacggc 660 gccatcggcg gcggcggccc gctcaacgtc ctcgacgcca cccgcggcaa cctgctcacc 720 gtgcactgcc aggagctgat gcgcgacgcc gacaccggca cgccgtggtt cgagccgccc 780 gccatcctgc gcgagcgcgg cgacgccctg tggcacgatc ccaaggcccc gcacgacccc 840 gccttcgacg aggccctgcg cgagcgcgtg ctcgaccgca tcctggccgt gctgctcgcg 900 cgcacagtgt tcgtgctcga tcacggcatc tgcgccgcca ccgagctcga ctggatgacg 960 cgcaccgcgc tcggcttccg caccggcttg gtcgacctgg tggacgaact cggccccgag 1020 cgcgtggccg agctgtgcca gcgctacgcc gccgagcacc ccggcttcgt catcccggac 1080 agcatccgcg agcagcacaa gccgcgcttc tacggcaacc tgcgcgtcac ccgccaggac 1140 gagctggcca tcgtgcgcat cttccgcccc gaggtgaaga acgcgctcga ccgccgcacc 1200 ctgagcgagc tcgaccacct catggccgcg ctgtcggccg acgacagcgt cgagggcgtg 1260 gtcctgagca gcgccggcgg cgcgctggcc ggcgccgaca tcaccgagct agcgcgcgtg 1320 cgcaccaccg aggaggcggt gtccacctgc gctttcggac aagcggtctt gaaccgcatc 1380 gcggccatgg acaagcccgt ggtcgccgcc gtcgacggcc cggtgctggg cggcggcgcc 1440 gagctgtcga tggcgtgcca tgcgcgcgtc gtcggcccgc gcctgagcat gggccaaccc 1500 gaggtcaacc tcggcatcat ccccggctac ggcggcaccc agcggctgcc gcggctcatc 1560 ggcgtggagc gcgcgctggc catgatgcgc acggcgcaga gcatcgacgc gcagaccgcg 1620 tgcgagtggg gctgggccag cggcacgccg atggtcgact tcgtcggcgc ggccgcgacc 1680 ctcatccgca gccacctcgc cggcgaggcc gagctcgcgc cgctcgaccc cgcgcccatg 1740 agcgtacccg ccgcggccgc ccccgtggac atcggccacc gctcgcgcgt catcgacgag 1800 atcctcgtgg atgtggtcca gtccggcttg cgcgcgccgc tgagcgaggg cctggccacc 1860 gaggccgccg gcttcggccg ctgcgtgctc accgtggacc tcgacatcgg actcaagaac 1920 ttcatgcaga acggcccccg ggttccggcg ctgttcctcc acgagtag 1968 <210> 157 <211> 768 <212> DNA <213> Anoxybacillus flavithermus <400> 157 atgttttcta ttcaacaaga ggggtatgtg gcgattttag cacttcatcg tccaccagca 60 aacgctttag catcttctgt tttgaaagag ctttcagaac ggcttgatgc attaaaagaa 120 gacgaacaag tacgtgtcat cgttcttcac ggagaaggaa gatttttctc agctggtgcc 180 gatattaaag agtttacagc gatcgaggcg agcgaacaag cggctgaact tgctcgagct 240 ggacaacaag tgatggagaa aattgaacag tttccgaaac cgattattgc cgcgattcac 300 ggtgctgcac ttggcggagg gctcgagtta gctatgagtt gccatctgcg catcgtagcg 360 gaaaacgcca aacttggctt accagaattg cagctcggca tcattccggg atttgcagga 420 acacaacgct tattgcgtca tgtcggtatg gcaaaagcgc tagaaatgat gtggacaagc 480 gaaccgatca caggtgcaga agctgtgcag tggggactag caaacaaagc cgtcccagaa 540 gaacaattgc ttgatacagc gaagcaactt gcacaaaaaa ttgctcaaaa gagcccgatt 600 tctgttcaag cggtattgaa actagttaat gaagctcgca caaaaacgtt ccatgaatgc 660 gttgaaaaag aggctcaact gtttggacaa gtctttgtaa cagaagatgc gaaagagggc 720 atttcggcat ttatcgaaaa acggacacca cagtttcaag gaaaataa 768 <210> 158 <211> 783 <212> DNA <213> Streptomyces avermitilis <400> 158 atgagcacgg cgcccgaagc tgccgacttg gtgctccacg agcgtcacgg cggcgtactg 60 accatcacca tcaaccgccc cgcgcagaag aacgccgtcg accacgaggc cgcggtacag 120 ctcgcggcgg ccgtggatct gctcgacgcg gacccggagc tgtcggtcgg cgtcctcacg 180 ggcgcgggcg gggtgttcag cgcgggcatg gacctgaagg cgttcgccaa gggcgagctg 240 cccttgctgc ccagccgggg cctgggcggg ctcacccgcg cgtcggtgcg aaagccgctg 300 gtcgccgcgg tcgagggctg ggcgctcggc ggtggcttcg agctggtcct cgcctgcgac 360 ctgatcgtcg ccgcggagga cgcccgcttc gggtttcccg aggtcatgcg tggtctcgtg 420 gcggcggagg gcggactggt caggctgccg cgccgacttc cgtaccacgt cgccgcgcgc 480 gtactgctga cgggcgagcc gctgaccgcc gtcgaagcca aggagtacgg gctcgtcaat 540 gagctgaccc cgcccggcgc cgcgctggac gcggcccggg agctcgcggg ccgcgtcgcg 600 cggaacgcac cgcttgcact ggcggccgtc aaggaggtcc tgcgcgagac acagggcctg 660 aaggagagcg acgcgttcag acgccaggac gagctcacga gcggactggc cgccagcgag 720 gacgcgcggg aaggcgcaca ggcgttcgcc gagaaacgcg ccccggtctg gcacggccgc 780 tga 783 <210> 159 <211> 1683 <212> DNA <213> Advenella kashmirensis <400> 159 gtggacaatg gccgtaagct gattgaacgt ggctggcatt tattcaaccg tatcgaaaag 60 ctagcctttc ctacactggc actcatgcac ggcccctgcc tgggtggcgg gctggaactg 120 gcactggcgt gccgttatcg aatcgcgatc gattctccca agccggtgat cggcctgcct 180 gaagtcaaat tgggcatctt ccccgcctgg ggcggcctga tgcgactacc ccgcctgatt 240 ggtccgcaaa ccgccctgaa catgatgctg accggtcgca cactggatgg ccgcaaggcc 300 aggtctgccg gtctggtaga tttgctggtc gcaccccgag ttgcagagaa atcggcgatc 360 gatctggtca cgtcgggcaa accggcgcgt caggctcgcg gcctggccgg cttgctcaat 420 cgtgcaccgt tcaagtcgct ggtggctgcc caggcacgca aaagcgtcaa gcaaaaagac 480 ccttatggcc actaccccgc caccctgacc atgctggatc tgtgggaaaa acatgatggc 540 gacccgttgg ccgatcccca ggcgctgacc cggctgctgc aatcggatgt cacccgcaat 600 ctgatccgtg tatttcacct gcaggagcgg ctcaaggcgt ttggcaagaa ggataatgcc 660 actcccgtca accatgttca tgtgatcggg gccggcgtga tgggcggtgg catcgctgcc 720 tggtgcgcgc tgcagggcat caaaaccacc ttgcaggata ccgacgccca gcgcatcgcc 780 ggggcgttca aaaacgccgt ctccatttat gcccgcaagg atcggtatac cgcgcaggca 840 gcccgcgatc gcctgattcc ggacctggcg ggccacggta tcgcgacggc tgatctggtg 900 attgaagcga tcagcgaaaa tccgcaagcc aagcaatcgc tctaccagca gatcgaacca 960 aaaatgaaag aaggcgccat tttagccacc aatacatcca gtctgtccat tgcgcagtta 1020 cgcagcgtgc tggtgcaccc cgaacgtttt gtcggtattc attttttcaa tccagtctca 1080 cgcatgccgc tggtagaagt ggtacatgcc gatggcatcg cccaggaaac tctggacacc 1140 gctgccgcct ttgtcggcaa aatcggcaaa ctgccgctgc cggttcagga cacgccgggc 1200 tttctggtca acgccgtgct tgctccctat atgctgcaag ccatgcggtg cattgacgaa 1260 ggcatggatc ccgaagtcat cgataccgca atgctggagt tcggcatgcc catggggccg 1320 atcacgctgg ccgatacggt tggtctggat attgccatgg cagccggcaa acagctgtcc 1380 gaaggccagg agccgccacg ctgcctgcaa gagaagattg cccaaggcaa gctgggtgtc 1440 aaaagcggcg aaggctttta cgtgtggaaa gaccgcaagc atgaccagcg cagtagcaaa 1500 gccatcccgc aaggcctggc acagcgcctg atcaagccgc tgatagagca gaccgaaaaa 1560 caacttgcga acaacatcgt gcaagatgca gatcttgccg atgcaggcgt gatattcgga 1620 accgggtttg cgccttttac cggaggaccc attcattaca aacaaagtaa aggaggacta 1680 tga 1683 <210> 160 <211> 714 <212> DNA <213> Oligotropha carboxidovorans <400> 160 gtgagccttt cgccgcttgc caacggcgta cgcgttctca cactggatcg tccgtccaag 60 gccaacgcgt tgaatgcgga ggtcgtggac cagttgcttg cgtgtgtcgc ccaggccgag 120 gcggaggatt gccgcgtgct gatcctcgcc gccaacggca aggcgttttg cggcgggttt 180 gatttcggtg gttatgaatc gatgtcggcg ggcgacctgc tgctgcgctt tgtccggatc 240 gaggagttgc tgcagcggat gcgccagtcg tcgtttgtca gcattgctct ggtgcatggt 300 gcggcgatgg gggcgggggc ggacatcgtc gcgtcttgca cctatcgcat cggcaccgac 360 gcaagccggt ttcgctttcc gggattccgt ttcggcgtgg cgcttggcac gcggcatctg 420 gcgcagcttg tcggcccgca acgggcgcgc gatatcctgc tgaccaatgc aacgatcgat 480 gcattgaccg ctgtcgatat cggattgctg acgcacctcg tcgatgccgg gagcatgcgg 540 cagaaagcgg acgagattat tgcgcagatt ggctcgctgg accgtgtcgc acgcaaccgg 600 attttgcatc tgacctcggc tcagaacaat gacggtgaca tggctgagct ggtgaaatcg 660 gtgagcgcgc ccgggctaca cgagcgcatt gcgcagtacc gcgccgggca ttga 714 <210> 161 <211> 801 <212> DNA <213> Riemerella anatipestifer <400> 161 atgtacaaat taatagatgt agataaccat tttgaaggaa agcttcaaat cgcatatatc 60 aatcagccag aatcgtttaa tagtcttaat aaggttgttt tagaagagtt attgcacttt 120 ataaaagctt gtgacgcaga ttctagtgta cgctgtattg caattagtgg caaaggtaag 180 gcgttttgtt ctggtcagaa tttaaaggag gctttagatt ataaagcaga agccaatgag 240 gaacgcttta tccaaaggat tgtgatagat tattataatc cgttagtgaa ggctattgtc 300 tatgctaaaa aaccagtaat tgcattggtt aatggtcctg cggttggtgc aggagcaatg 360 ttagctctca tctgtgattt tgcagtggcg tcagagtcag cgtatttttc cttagctttt 420 tctaatatag gactagtgcc agatacggca ggtacttact atttgcctaa acttttaggg 480 cgttccttag cgagttattt ggcatttaca gggaagaagc tatctgctaa agagtcttta 540 gaaagaggtt tggtggtaga tgttttttca gatgctactt tttcggaaca atctttacaa 600 gtcctagaac atattactca tcagcctact gtggcattgg ggcttacaaa aaaagccttt 660 aataaatctt atcagaatag tctatcggag cagttagatt tggagagtat tctccagcaa 720 gatgctgcag aaacttggga ttttcaagag gggatagccg cttttttagc aaaaagaaaa 780 cctcagtata aaggtaagta a 801 <210> 162 <211> 1269 <212> DNA <213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007 <400> 162 atgtcagaaa caatcaattt agatgaaatg tcagcaaaac aattattggg ttattatcaa 60 gaaaaattgg atgaagaagc aagacaggca aaaagagaag gaaaattagt ttgttggtct 120 gcttccgttg ctccaccaga attctgtgta gctatggata ttgccatggt gtatccagaa 180 actcatgcag cagggattgg agctagaaaa gggtcgttag atctgctaga agtagcagat 240 gaaaaagggt attctttaga tatttgttct tatgcaagag taaatttggg gtatatggaa 300 ttgttaaaac aacaagcctt aactggagaa actcctgaaa aattagcaaa ctctccggct 360 gcaaaagttc ctttaccgga tttagttatt acatgtaata acatttgtaa tactttgtta 420 aaatggtacg aaaatttggc aaaggaatta aatattccat gtattgtaat tgacgttccg 480 ttcaatcata ctatgccaat tacaaaacat tcaaaagaat atattgcaga tcaatttaaa 540 tatgcaattc aacaattaga agaaattaca ggaaagaaat ttgactatga taaattctta 600 gaagtgcaag agcaaacaca aagatctgta tatcaatgga atcgtttagc agctcttgct 660 cactacaaac cttctccatt aaatggtttc gatttattta acttcatggc tttaattgta 720 tgtgctagaa gtagagatta tgcagaaatc actttcaaga aatttgcaga tgaattggaa 780 gaaaacttga aaaatgaagt atatgcgttc aaaggagctg aaaagaacag agttacttgg 840 gaaggaattg cagtatggcc ttaccttgga cacactttca agtctttaaa aggaatggga 900 agtatcatga ccggttctgc atatccagga atctggaact tgacatatac tcctggagat 960 atggaatcta tggcggaagc atatacaaga gtctacatta atacttgctt acaaaataaa 1020 gcggatgtcc tttctaaaat tgtaacagac ggaaaatgtg atggaatact atatcatttg 1080 aatagaagtt gtaaactgat gagtttcttg aatgtggaaa ctgctgaatt agttgaaaaa 1140 gcgactggag tgccatatgt aagtttcgat ggagaccaaa cagatccgag aaatttcgca 1200 ccggctcaat ttgatacaag agtacaagct ttaaatgaaa tgatggaagt taataacgaa 1260 acaaaataa 1269 <210> 163 <211> 834 <212> DNA <213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007 <400> 163 gtgcaagatg acagaagttt taagaaagga aagagaagag gaatgtatac agttggagtg 60 gatataggtt cttcttcttc aaaagtagtg atattaaagg atggaacaga gattgtaagt 120 caatcggcaa ttcagtcggg aattggaagt aatcgagcca ttgttgcttt ggaagataat 180 ttaaaaaaag caaacttgac gaaggaagat attggtttta cagttgttac tggatatgga 240 cgctttactt ttgaaggagc agataaacaa atcagcgaga ttagttgtca tgccaggggg 300 attcattttt tattaccgaa tgtgagaacc attattgata ttggtggaca agatgccaaa 360 gcgatcagct tagatgaaaa aggtcatgta agacaatttt ttatgaatga caaatgtgca 420 gcaggaacag gacgattttt aactgtaatg gcacgcgtac tagagatttc cctagatgag 480 atgggaactt atgatgctct ttctaaaaat ccttgtaata ttagtagtac ttgtgctgta 540 tttgcagaat cagaagtcat ttctcaattg gcaaagggaa ataccaaaga ggatgtcatt 600 gcaggagtac ataattctgt cgctcataag atattaggtt tagtatatcg tacttctatg 660 gaagaaaaat ttgcgatttg tggtggtgtt gctcagaata caggtgcatt gcgtgcaata 720 cgggaagctt tgaaaaaaga agtaatcgtt gctcctaatc cacaattaac aggagcatta 780 ggagctgcaa tttttgctta tgatgagctg aaaaaattaa gaaagggtga ataa 834 <210> 164 <211> 1125 <212> DNA <213> Fusobacterium necrophorum subsp. funduliforme Fnf 1007 <400> 164 atgaaaggca gattagaaga attaattcat atatttgaag atgttgcaaa caaccccaaa 60 aaaatggtag cagaatataa aaaagaagta gggaaagaag tgattggagt catgccagta 120 tatgctccag aagaaattat tcacgctgct ggatgtttac ctattggatt atggggagga 180 aaaaaagaag tttctaaagc aagagcatat ttacctcctt ttgcatgttc tattatgcaa 240 actgttatgg aattacaaat tggaggaaca tatgacattt tagatgcagt attattctct 300 gtaccttgtg atactttgaa atgtttaagt caaaaatgga aaggaaaatc tcctgtaatt 360 gtatttactc atcctcaaaa cagagtaatt gaaggagcaa atgcttactt agtaaaggaa 420 tatcaagcag taaaagaaaa attagaagga atcttaggaa gaaccattcc tatggaagcg 480 attgaagaaa gcgtaaaagt atataatgaa aatagaagag ttatgagaga atttgtagaa 540 gtggcggcac aatatccaca aattatcgat ccaattgtta gacataatgt gatgaaatcc 600 agatggttct taagaaaaga aaaacatact gaatatgtaa aagaattaat cgctgaatta 660 aaaaaagaaa ctattgttcc ttgggacgga aagaaagtaa tcttaacagg aattatgaca 720 gaaccagtag aattgttgca aatctttaaa gatgaaaaac ttgctattgt agccgatgat 780 ttagctcatg aaagccgaca atttagagga gatgttcctg aagaaggagg agatgttcta 840 tacagaatgg caaaatggtg gcaaaattta gaaggatgtt ctttagcaac ggatactaat 900 aaaggtagag gacaaatgct aatggatatg tgtaaggata cgaaagcaga tgccgttatc 960 gtgtgtatga tgaaattctg tgatcctgaa gaatttgact atccggtata ctatagagaa 1020 tttactgaat ccggaattaa aaatattaca gtggaagtgg acttagaagt ttcttctttt 1080 gaacaaatta gaacaagaat acaaacattt aaagatattt tataa 1125 <210> 165 <211> 1269 <212> DNA <213> Desulfosporosinus youngiae DSM 17734 <400> 165 atgacggata caacaactat gagtgccaaa gaattgttag gtttctatca ggaagaattg 60 tatgaagaag cgagacaggc caaaaaagaa ggaaaacttg tttgttggtc tgcatcggtt 120 gctccttcgg agttttgtgt ggctatggat gtggcgatga tctatcctga aacacatgct 180 gcggggattg gggcaagaaa aggtgcctta gatgtgctgg aagttgccga tgaaaaaggc 240 tataacctgg atacttgctc ctatgcaaga gtcaatatgg gttatatgga acttctgaaa 300 caagaggctt taacaggaat aacgccggaa aagcttgaaa aatccccggc ggccagaata 360 ccgctgcccg attttgtcat aacctgcaac aacatttgca acaccttgct taagtggtat 420 gagaatcttg ccgttgaatt aaatattccc tgcatcatca ttgatgttcc ctttaatcat 480 accatgccca ttccccagta tgctaaggac tatattgcgg aacagtttaa ggaggctatt 540 actcagcttg aggaaatttg cggcaggaaa ttcgactacg acaaattttt gaaagtacag 600 gaacaaaccc agcgttctgt ggcccagtgg aacagaattg ctgctttgtc gggacataaa 660 ccatctcctt taaatggttt tgatcttttc aactatatgg ccctgatcgt ttgtgccaga 720 agcagagact acgcggaaat tacctttaaa aagtttgccg atgaacttga agaaaacctc 780 aaaaacggta tctacgcctt taaaggaaat gaacaaaagc gtgtaacttg ggagggcata 840 gctgtttggc cgcatctggg ccatacattt aaaggcttaa agaatctggg caatatcatg 900 acaggttcgg cttatcccgg tttgtggaat cttacctaca cacctgggga tatgagttcc 960 atggcggaag cttataccag aatttatatc aatacttgtc tcgataacaa agttaaggtg 1020 cttagtgacg tcatcagcgg cggaaagtgt gacggggtta tttatcatca gaacagaagc 1080 tgtaagctca tgagtcttct caatgtcgaa acggctgata tactccaaaa acaaaatcat 1140 ttaccctatg tcagctttga tggggaccaa acggatcctc gtaactttgc tcctgcccag 1200 tttgatacac gtatccaggc cttagatgaa atgatgaagc agaataagga gggagtttcc 1260 aatgagtag 1269 <210> 166 <211> 1119 <212> DNA <213> Desulfosporosinus youngiae DSM 17734 <400> 166 atgagtagaa ttgaaacgat tatcagtgaa ttaacgtcca ttgccaataa tccccgccag 60 gctatggaag attataaaaa agaaaccggc aaagggtcgg ttggggttat gccttattat 120 gctcctgaag aaatcattca tgccgcaggg tatctgcccg taggtatttg gggaggacaa 180 aagagtattt ccaaggcccg ggcctatttg cctccctttg cttgttcaat tatgcaatcc 240 gtggtggaaa tgcagcttga aggggtctat gacgatttag aagcggtcct tttccctgtt 300 ccttgtgaca ccttaaaatg tcttagccaa aaatggaaag gaacctcccc tgtcatcgtt 360 ttaactcatc ctcaaaacag aaaactggaa gcagccaata agtttcttgc tgaggaatat 420 aggcttgtgc gtgaaaaact ggaaaaaatc ctgaatgtta agattacaga cgaggcactt 480 aaccaaagca ttgaaattta taacgaaaat cgtaaagtaa tgcgtgaatt tacagagata 540 gctgctaatt atcccaacat tattgatccc gtaaaacgtc atgcgcttat caaagccaga 600 ttctttatgg aaaaagccaa acataccgct ctggtcaaag aattgaatgc agagcttaaa 660 gcgttaccgg tggaagcctt tacaggcaaa aaggttgttt tgacaggcat tatggctgaa 720 cccaatgaag tattggacat tttgcaagat aacggttttg ctgttgtggc agatgacctg 780 gcccaggaat ccagactgtt cagaaatgat gttccctcag ggacagaccc actctatcgc 840 ttggctaaat ggtggcagga attcgatggt tgttctctgg ctgtcgatgc gaaaaaacca 900 agaggcccca tgctgatgga tatggttaaa gcatctaagg ccgatgccgt tgtggtttgc 960 atgatgaagt tctgtgaccc tgaagaattt gactatccaa tctactacag acagtttgaa 1020 gaagccggaa ttaagagctt atttatagaa attgacctgg aaccaacctc ctttgaacag 1080 actaaaacca gagttcaaag ttttagagaa atgctgtga 1119 <210> 167 <211> 819 <212> DNA <213> Desulfosporosinus youngiae DSM 17734 <400> 167 atgtttacaa tggggattga tattgggtcc tcatcctcaa aggttgtaat ccttgaagat 60 ggagttaata ttatcgccgg agaagttatt cagattggaa caggttctac gggacctaaa 120 cgtgtactgg atgaagctct tgccaaagca ggtcttacat tgcaagacat ggctaaaatt 180 attgctacag gctatggaag atcgtctgtg gaagaagcac acaaacaaat cagcgaaatc 240 agttgtcagg ctaagggagt tttcttttta gttccttcag caaaattaat tattgatatt 300 ggcggtcagg atgttaaggc cattaaactt gacagtaaag gctgtgttaa gcagtttttt 360 atgaatgata aatgtgccgc cggaacagga cgttttctcg atgttatgtc gcgggtactg 420 gaagttaatc ttgatgaaat ggcggaatac gatgcccggg caacagaacc tgccacggtc 480 agcagcactt gcacagtttt tgcagaatct gaggtaatat ctcagcttgc caacggagtt 540 gctaaagaga acattattgc aggggttcac cagtcagttg ctagcaaagc ctgtggactt 600 gcctatcgat gtggggtgga agaggacatc gtgatgtgcg gaggcgttgc taaggactta 660 ggggttgtca gagcaatcag caaagaactg aaaaaaccgg tcattgtagc tcctaatcca 720 caaattacag ctgcacttgg agctgctata tttgccttcg aagaagttat ggaaactgtt 780 atggttgcct tcgaagaagt taggggagct aataaataa 819 <210> 168 <211> 1269 <212> DNA <213> Peptoniphilus indolicus ATCC 29427 <400> 168 atgaatacta tagatatatc aaatatgaaa gctaaagaaa tgcttggata ttttcaaaac 60 aaacttgacg aagaagcacg tgaagctaaa aaaaatggaa aattagtttg ctggtcagcc 120 tctgtagctc catctgaatt ttgtgtaacc atggatatcg cattagttta tccagaaact 180 cacgcagccg gtataggtgc tagaaaaggc tctttagcta tgttagatgt tgctgataga 240 aaaggttata atacagatat atgttcttat gccagagtaa acttaggata tatggaactt 300 ttaaaagaat atgctaagac aggagtgaaa cctaaagaac ttgaagaatc tcctgctgca 360 gatgttcctc tacctgattt agtaataact tgcaataata tatgcaacac tttactaaaa 420 tggtatgaaa atttagctgc agaattaaat attccttgta tagttataga cgttcctttt 480 aatcatacta tgcctattcc taagtattct aaagaatata ttgctgacca atttaaggaa 540 gcaataagac aacttgaaga aataacagga aaagattttg actatgataa atttttagaa 600 gttcaagagc aaacgcaaag atctgttgct caatggaata gacttgctgc actttctaaa 660 tatgaaccgt ctcctctaaa tggatttgat ttatttaact atatggctct tatagtttgt 720 gcaagaagta aaaattatgc tgaattaact tttaaaaaat ttgccgatga acttgaagaa 780 aatatgcaaa atggagtgta tccttacaag gctggagaac aatccagaat tacttgggaa 840 ggtatagcta tttggccata tttaggacac acttttaaga ctcttaaagg ctatggctca 900 ataatgacag gctctgctta tcctggactt tggaacttag aatacacacc tggagatatg 960 ctttcaatgg cagaagctta tacgagaata tatataaaca cttgccttga caataaagtt 1020 gatgtattga gaaaaatcat taaaaacggt aaatgtgatg gggtcgcata ccatctaaat 1080 agaagttgta aattgatgag tcttctaaac gttgagacag ctgaaatttt aaataaagaa 1140 aataatcttc catatgttag ttttgatggt gatcaaactg atcctagaaa tttctcagaa 1200 gcacaatatg ataacagaat acaaactctt actgagatga tgtctgccaa taaaaaaatg 1260 aggggttga 1269 <210> 169 <211> 792 <212> DNA <213> Peptoniphilus indolicus ATCC 29427 <400> 169 atgtacacta tgggagtaga tatcggttct acatcatcta aaatcataat acttgaagat 60 ggaataaaaa ttatcggaaa tattgtagta caatctggaa ccggtacaag tgggccaaca 120 attgctactg caaaagctaa gtcctttctt tcaaataata atttaacttt agatgatata 180 tctaaaatcg ttgtcacagg ttacggcaga ttttcatttg atattgccga taaacaaata 240 agtgaaataa cttgtcatac aaaaggtatt aactttttag tgcctgaagc tcgaactatt 300 ttagatatag gtggacaaga tacaaaagct atttcagtta atgataaagg tcaagttcta 360 caatttttca tgaatgacaa atgtgccgcc ggcactggca gatttttaga agtcatggct 420 aaaattttag aaataccttt agaaaaaatg ggtgaatatg atagattatc aactaatccg 480 gtagctataa gtagtacttg taccgttttt gctgagtctg aagttatttc tcagctatca 540 aagggcatat ctaaagaaaa tatattagcc ggtgtacata attcaactgc taacaaagtt 600 tgtggtcttt tatatcgtac aggaattaag gaaaaaatag ttttatgtgg aggagttgct 660 caaaaccaag gtgttgttag agcgctccaa gaggaattaa aaaaagaaat aaccatagct 720 cctcacccac aaatgacagg cgccataggt gctgctttat ttgcttatga agaggcgaat 780 aaaaatttat ag 792 <210> 170 <211> 1119 <212> DNA <213> Peptoniphilus indolicus ATCC 29427 <400> 170 atgaacaaaa ttaatgaaat aataaattta ttggatgaag tttctaaaga tcctaaacta 60 acagttaaaa aatataaaga aaaaacagga aaaggtgttg taggtgtcat gccattatat 120 gcacctgaag aaattattca tgctgcaggt tttctaccta tgggactttg gggtgcacaa 180 aaagaagtat ctaaagcaag aatttattta cctccttttg catgttcaat aatgcaaact 240 aatatggaac ttcaaataga aggtgcctat gatgacttag atgcagttgt attttctgta 300 ccgtgcgata ctctaaaatg tatgagtcaa aaatggaagg gtaaaagtcc tgttatagta 360 tttactcatc ctcaaaacag aaaattagaa tctgcaaata aatttttggt tacagaatat 420 gaaatcttaa aagataaatt agaaaagata ttaaatgtaa aaatatctga tgaatccata 480 acaaatagta ttgaaattta caatgaaaat agaaaagtca tgagagaatt ttcagaccta 540 gctggtcaat atcctaatat aattgaccct attcaaagac atattgtatt taagtccaga 600 tggtttatgg aaaaatcaga acatactaaa ttagttaaag aactaatatc tgaaattaaa 660 aaattaccta ttgaagaatg ggatggctat aaagttatag caactggtat tatgatagaa 720 cctgaagaaa tacttcaaat atttaaagat aagaaaatag ctattgttgc agatgattta 780 gctcaagaat caagacaatt tagacatgac gtacctgaag gagatcaacc tcttttaaga 840 cttgctaagt ggtggcaaaa tttagaagga tgtgctcttg caactgatac aaaaaaatta 900 agaggccaaa tgctaattga tatggcgaaa aaatataatg ccgatgctgt attgatatgt 960 atgatgaaat tctgcgatcc tgaagaattt gactaccctg tatactatag agagttccaa 1020 gaagctggca taaagaattt actaattgaa attgacttag aaatgacagc ttttgaacaa 1080 actaacacaa gacttcaaac tcttgtagaa actctctaa 1119 <210> 171 <211> 1269 <212> DNA <213> Desulfosporosinus meridiei (strain ATCC BAA-275/DSM 3257/ NCIMB 13706/S10) <400> 171 atgactgata caacagctat gagcgccaaa gaattgttag gtttctatca ggaagaattg 60 tatgaagaag cgagacgggc aaaaaaagaa ggaaaacttg tttgttggtc tgcatccgtt 120 gctccttcgg agttttgtgt ggctatggat gtagctatga tatatcctga aacccatgct 180 gcgggtattg gggccagaaa aggtgcctta gatgtgcttg aagttgcgga tgaaaaaggc 240 tataacgtgg atacttgctc ctatgcaaga gtaaatcttg gttatatgga acttttaaaa 300 caggaggctt taacaggaat aacaccggaa aagcttgaaa aatccccagc ggccagaata 360 ccccttcccg attttgtcat aacctgtaac aacatttgta acaccttgct taagtggtat 420 gagaatcttg ccgttgaatt aaatattcct tgcatcatca ttgatgttcc ctttaatcat 480 acaatgccca ttccacagta tgccaaggat tatattgcgg aacagtttaa ggaagctatt 540 actcagcttg aggaaatttg cggcaagaaa ttcgactatg acaaattttt aaaagtacag 600 gaacaaaccc aacgttctgt tgcccaatgg aatagaatcg ctgctttgtc atcacataaa 660 ccatcccctt taaatggttt tgatcttttc aactatatgg ccctgatcgt ttgtgcaagg 720 agtaaagact acgcagaaat tacctttaaa aagtttgctg atgaacttga agaaaatctt 780 aataagggta tcttcgcctt taaaggaaat gaacaaaagc gggtaacttg ggaaggcata 840 gctgtttggc cgcacctggg acatacattt aaaggcttaa agaatcttgg caatataatg 900 acaggttcag cctatccggg tctgtggaat gttagttata caccaggtga tatgagttca 960 atggcggaag cttatactag aatttatatc aatacttgtc ttgataataa agttaaggtt 1020 cttagtgacg taattagtgg cggaaagtgt gacggtgtta tttatcatca gaacagaagc 1080 tgtaagctca tgagttttct gaatgtagaa actgctgata tcctccaaaa agaaaatggt 1140 ttaccctatg taagctttga tggagaccaa actgatcctc gtaacttttc tcctgcccag 1200 tttgacacac gtatccaggc cttagatgaa atgatgaagc agaataagga gggagtttcc 1260 aatgagtag 1269 <210> 172 <211> 1119 <212> DNA <213> Desulfosporosinus meridiei (strain ATCC BAA-275/DSM 13257/NCIMB13706/S10) <400> 172 atgagtagaa ttgaaactat tattagtgaa ttatcttcaa tttcaaataa tccccgcaag 60 gctatggaag attataaaaa agaaaccggt aaagggtcgg taggggttat gccttattat 120 gcccctgaag aaataattca tgctgctggt tttcttcccg taggtatttg gggaggacaa 180 aagagtattt caaaagcccg tgcctattta cctccctttg cttgttcaat tatgcaatca 240 gttatggaaa tgcagcttga aggggtatat gacgatttag aagcagtact tttccccgtt 300 ccttgtgaca ctttaaaatg tctcagccaa aaatggaaag gaacatcacc tgtcatcgta 360 tttactcatc ctcaaaacag aaaactcgaa gcagccaata agtttcttgc tgaggaatat 420 cgacttgttc gtgaaaagct ggaaacaata ttgaatgtaa agattactga tgaagcactc 480 aaccaaagta ttgaaactta taacgaaaat cgtaaagtaa tgcgtgaatt tacggaccta 540 gctgctaatt atcctcagat tattgatccc agaatacgtc atgcaattat aaaagctaga 600 ttttttatgg aaaaatctaa acataccgct atggtaaaag aattgaattc agagcttaaa 660 tcgttacctg ttgaagcctt tacaggtaaa aaggttgttt taacaggaat tatggctgaa 720 cccaatgaag tattagacat tttaaaagat aacggttttg ctgttgtggc agacgacctg 780 gcccaggaat ccagactgtt cagaaatgat gttccgtcag gtacagaccc actatatcga 840 ttggctaaat ggtggcaaga attcgatggt tgttctcttg ctacagatgc gaaaaaatca 900 agaggcccca tgctgatgga gatggttaaa gggtctaagg ccgatgcagt tgtggtttgc 960 atgatgaagt tctgtgaccc tgaagaattt gactatccaa tctactatag acagtttgaa 1020 gaagctggaa ttaagagcct atttatagaa attgacctgg aaacaacatc ctttgaacag 1080 actaaaacca gagttcaaag ttttagtgaa atgctgtga 1119 <210> 173 <211> 786 <212> DNA <213> Desulfosporosinus meridiei (strain ATCC BAA-275/DSM 13257/NCIMB 13706/S10) <400> 173 atgtttacaa tggggattga tattgggtcc tcatcctcaa aggttgtaat acttgaagat 60 ggagttaata ttatcgctgg agaagtcatt cagattggaa caggttcgac aggacctaaa 120 cgtgtactga atgaagctct ttccaaagca ggtcttaaat tggaagacat ggctaaaatt 180 attgctacag gctacggaag atcttctgtg gaagaagcac acaaacaaat tagcgaaatc 240 agttgtcagg ctaagggagt tttcttttta gttccttcag caaaattaat tattgatatc 300 ggcggtcaag atgttaaggc aattagactt gacagtaaag gcggcgttaa gcagtttttt 360 atgaatgata aatgtgccgc cggaacagga cgttttctcg atgttatgtc acgagtactt 420 gaagttaatc ttgatgaaat ggcagaatac gatgctcgtg caacagaacc tgccacggtc 480 agcagcactt gcacagtttt tgcagaatct gaggtaatat ctcagctttc caacggagtt 540 gctaaagaga atattattgc aggggttcac cagtcagttg ctagcaaagc ctgtggactt 600 gcctatagat gtggggtgga agaggacatt gttatgtgcg gaggtgttgc taaggactta 660 ggggttgtcc gggcaataag caaagaacta aaaaaacctg tcattgtagc tcctaatcca 720 caaattacag ctgcccttgg agctgctatc tttgccttcg aagaagtcag gggagctaat 780 aaataa 786 <210> 174 <211> 1434 <212> DNA <213> Acidaminococcus fermentans <400> 174 atgccaaaga cagtaagccc tggcgttcag gcattgagag atgtagttga aaaggtttac 60 agagaactgc gggaaccgaa agaaagagga gaaaaagtag gctggtcctc ttccaagttc 120 ccctgcgaac tggctgaatc ttttcggctg catgttgggt atccggaaaa ccaggctgct 180 ggtatcgctg ccaaccgtga cggcgaagtg atgtgccagg ctgcagaaga tatcggttat 240 gacaacgata tctgcggcta tgcccgtatt tccctggctt atgctgccgg gttccggggt 300 gccaacaaaa tggacaaaga tggcaactat gtcatcaacc cccacagcgg caaacagatg 360 aaagatgcca atggcaaaaa ggtattcgac gcagatggca aacccgtaat cgatcccaag 420 accctgaaac cctttgccac caccgacaac atctatgaaa tcgctgctct gccggaaggg 480 gaagaaaaga cccgccgcca gaatgccctg cacaaatatc gtcagatgac catgcccatg 540 ccggacttcg tgctgtgctg caacaacatc tgcaactgca tgaccaaatg gtatgaagac 600 attgcccgtc ggcacaacat tcctttgatc atgatcgacg ttccttacaa cgaattcgac 660 catgtcaacg aagccaacgt gaaatacatc cggtcccagc tggatacggc catccgtcaa 720 atggaagaaa tcaccggcaa gaagttcgat gaagacaaat tcgaacagtg ctgccagaac 780 gccaaccgta ctgccaaagc atggctgaag gtttgcgact acctgcagta caaaccggct 840 ccgttcaacg ggttcgacct gttcaaccat atggctgacg tggttaccgc ccgtggccgt 900 gtggaagctg ctgaagcttt cgaactgctg gccaaggaac tggaacagca tgtgaaggaa 960 ggcaccacca ccgctccctt caaagaacag catcgtatca tgttcgaagg gatcccctgc 1020 tggccgaaac tgccgaacct gttcaaaccg ctgaaagcca acggcctgaa catcaccggc 1080 gttgtatatg ctcctgcttt cgggttcgtg tacaacaacc tggacgaatt ggtcaaagcc 1140 tactgcaaag ccccgaactc cgtcagcatc gaacagggtg ttgcctggcg tgaaggcctg 1200 atccgcgaca acaaggttga cggcgtactg gttcactaca accggtcctg caaaccctgg 1260 agcggctaca tgcctgaaat gcagcgtcgt ttcaccaaag acatgggtat ccccactgct 1320 ggattcgacg gtgaccaggc tgacccgaga aacttcaacg cggctcagta tgagacccgt 1380 gttcagggct tggtcgaagc catggaagca aatgatgaaa agaaggggaa ataa 1434 <210> 175 <211> 1140 <212> DNA <213> Acidaminococcus fermentans <400> 175 atggctatca gtgcacttat tgaagagttc caaaaagtat ctgccagccc gaagaccatg 60 ctggccaaat ataaagccca gggcaaaaaa gccatcggct gcctgccgta ctatgttccg 120 gaagaactgg tctatgctgc aggcatggtt cccatgggtg tatggggctg caatggcaaa 180 caggaagtcc gttccaagga atactgtgct tccttctact gcaccattgc ccagcagtct 240 ctggaaatgc tgctggacgg gaccctggat gggttggacg ggatcatcac tccggtactg 300 tgtgataccc tgcgtcccat gagccagaac ttcaaagtgg ccatgaaaga caagatgccg 360 gttattttcc tggctcatcc ccaggtccgt cagaatgccg ccggcaagca gttcacctat 420 gatgcctaca gcgaagtgaa aggccatctg gaagaaatct gcggccatga aatcaccaat 480 gatgccatcc tggatgccat caaagtgtac aacaagagcc gtgctgcccg ccgcgaattc 540 tgcaaactgg ccaacgaaca tcctgatctg atcccggctt ccgtacgggc caccgtactg 600 cgtgccgctt acttcatgct gaaggatgaa tacaccgaaa agctggaaga actgaacaag 660 gaactggcag ctgctcctgc cggcaagttc gacggccaca aagtggttgt ttccggcatc 720 atctacaaca cgcccggcat cctgaaagcc atggatgaca acaaactggc cattgctgct 780 gatgactgcg cttatgaaag ccgcagcttt gccgtggatg ctccggaaga tctggacaac 840 ggactgcatg ctctggctgt acagttctcc aaacagaaga acgatgttct gctgtacgat 900 cctgaatttg ccaagaatac ccgttctgaa cacgttggca atctggtaaa agaaagcggc 960 gcagaaggac tgatcgtgtt catgatgcag ttctgcgatc cggaagaaat ggaatatcct 1020 gatctgaaga aggctctgga tgcccaccac attcctcatg tgaagattgg tgtggaccag 1080 atgacccggg actttggtca ggcccagacc gctctggaag ctttcgcaga aagcctgtaa 1140 1140 <210> 176 <211> 783 <212> DNA <213> Acidaminococcus fermentans <400> 176 atgagtatct ataccttggg aatcgatgtt ggatctactg catccaagtg cattatcctg 60 aaagatggaa aagaaatcgt ggcgaaatcc ctggtagccg tggggaccgg aacttccggt 120 cccgcacggt ctatttcgga agtcctggaa aatgcccaca tgaaaaaaga agacatggcc 180 tttaccctgg ctaccggcta cggacgcaat tcgctggaag gcattgccga caagcagatg 240 agcgaactga gctgccatgc catgggcgcc agctttatct ggcccaacgt ccataccgtc 300 atcgatatcg gcgggcagga tgtgaaggtc atccatgtgg aaaacgggac catgaccaat 360 ttccagatga atgataaatg cgctgccggg actggccgtt tcctggatgt tatggccaat 420 atcctggaag tgaaggtttc cgacctggct gagctgggag ccaaatccac caaacgggtg 480 gctatcagct ccacctgtac tgtgtttgca gaaagtgaag tcatcagcca gctgtccaaa 540 ggaaccgaca agatcgacat cattgccggg atccatcgtt ctgtagccag ccgggtcatt 600 ggtcttgcca atcgggtggg gattgtgaaa gacgtggtca tgaccggcgg tgtagcccag 660 aactatggcg tgagaggagc cctggaagaa ggccttggcg tggaaatcaa gacgtctccc 720 ctggctcagt acaacggtgc cctgggtgcc gctctgtatg cgtataaaaa agcagccaaa 780 taa 783 <210> 177 <211> 1011 <212> DNA <213> Carboxydothermus hydrogenoformans <400> 177 atgaaattaa actatttttg cagttactgg ccggtggaaa tatccgaagg agcggggatt 60 tctacggtcc gttatttccc gtccgatgaa agcaaagctc cggtaaggct tcctgcttac 120 tgctgttctt atgccagggg aagccttgcc gaaattgaag aagaaggaga cggtgacttt 180 tggggatttg cccacagttg cgacacgatg cagagtttat acggcattac taagagttta 240 ctgggagacg accgggtttt tcttttcgtt ccgccggttg acttaaccac cgcttttgcc 300 cgggaatact accgggaagc tttaatttat ctctggcggg aactttccca aaaaagcggg 360 gttaatggtg aggaaaagtt aaagcttacc tgggaaaagt tgaaggagtt aagaaataag 420 gttaaatctt tggaaaactt gacgtcaatt attccttcct ccgaaatttt tgagctttta 480 aaaaagcttc agaccctgcc gctggatgag gctttggatt acctcgaggc caaaaaagcg 540 gaatttacca gtttatctgt ggctcaaaag gctataggga ttattttaac gggagcggta 600 gtcactaaca gtaaacttta ccttgcttta gaacaacagg gatttagagt agtttatgat 660 gatacctgta ccggctttcg tcattttgct ggagagatag aggataaaga cgatattttg 720 gaggcaatag tttcttacta cctttcaaag cccccctgtc cctgcaggca taagggagta 780 tgggcgaggg cggagtattt aaaaaatctt tatcataaca aaaatgcccg ggccattgta 840 cttttacaaa ataaattttg tgaccccttt gcctgggatg ttccctattt agtggactac 900 tttaaaaaac agggagttcc ggttttagtt ttagaggtgg aaggcggaga aatcggcgag 960 caaaataaaa ctcgcctcca ggccttccgg gaaagcgtgg gtggagtgta a 1011 <210> 178 <211> 1215 <212> DNA <213> Carboxydothermus hydrogenoformans <400> 178 atggctaaaa aaatctttaa gcctcttaag gcttcagaga aaataaataa aattttaaaa 60 aatcattatt taaaagcaaa gtatttgcca acgcttggaa aattttttgg ttataaaacc 120 gcctggatta ccagcggagc tccggtggaa ctactgcggg cctttggtat agagccggtt 180 tatccggaga attacggtgc catttgcggt gcccgcaagg tttcgccgag tctttgccag 240 gtagcggaaa acaggggtta ttctctcgat ttgtgttctt atgccaagag taatctcgga 300 agtatctgga atccgaaaga aagtccattt aacggcttac cccggccgga tttactggtg 360 gtttgcaaca acatttgcgg gacggtttta aagtggtacg aaactttaag ccgggaattt 420 aatattcccc tttttatcat tgatacccct tttatcaccg gtgaacccca accctggcaa 480 atccagtatg tggccaaaca gatagaaaaa ctggcgattg aactggaaaa atttttccgg 540 aaaaagttgg atttaaaccg tttggaaaaa gtaattctcc ttgccaatga gacggtggat 600 ttatggaagg ggataagaaa ttttgccaaa aataaacctt cgccggtaaa cgttaccgat 660 ttatttatta atctggggcc aatggtggtt ttaaggggta ccgaagttgc ccgggatttt 720 tacgaggaag tttaccggga agtggaagaa aggtacaaag ccggggttcc ggcggtagag 780 ggagaaaaat accgtttagt ctgggacaac attcccatct ggtacggact gtaccgtttt 840 tacggttatt ttgccgaaag gggagcggtt tttgttaccg attcctatac cggtggctgg 900 gcggtcaaca taaaaaaggg tcctcccttt tatgcattag ccgagaccta tgccggcgtc 960 tttttaaatc gggatttaga atttcgcaaa aatcagttgc aatctttcat tgaggaattt 1020 tctgccgatg gctttgtcat gcactccaat cgttcgtgca aagcttattc ttttgtgcag 1080 gaggaaatcc ggcgccaaat catgaggtca ctaggagtgc cggggttaat agtggatgcc 1140 gatatgaccg acagccggct ttattccgaa gaaacggttt taaaccgggt ccaggctttc 1200 ctggagagcc tgtag 1215 <210> 179 <211> 765 <212> DNA <213> Carboxydothermus hydrogenoformans <400> 179 ttgtatcttg gagttgatat tggttcgctt acgaccaagg ttgtcttaat tgaccgggga 60 aaaaatctta ttgcttatcg ttacagtaaa accggacctg ccggaaagga aacggccgag 120 cggttaattc aagaggtttt gataaaagcg aatatttccc gggacgatat tcagggaata 180 gttgctaccg gttacggcag ggttctcttt tccggaaagg agttttcgga gataacctgt 240 caggcccggg ggattgggca tttatacccg gaggcaaaaa cgattatcga tattggtggc 300 caggatagca aagtaatttc tctgggaaaa aacggaaagg tactggactt tgccatgaac 360 gataaatgtg ctgctggcac cggacgtttt ttggaggtga tgagtcaggc ccttgaagtt 420 cgtctggaag agatagggga acttgccgaa aagagccagg aggcagctaa gatatcttcg 480 gtttgtaccg tttttgccga atcggaagtg atatccaatt tatcccgggg gcagagccgg 540 gaagcggtag cacggggaat ttgtgaggcg gtggcggccc gaacggctat actggcgcaa 600 aaagtggggg tggtagaacc ggtggttttt accggagggg tggccaaaaa tactggagtt 660 gtggcggctt tggagcgaaa gcttggggtt aagttattaa ttccggaaga ttccacgatt 720 accgcagctc tgggggcggc tttattagcc gctgaaaatt cttaa 765 <210> 180 <211> 786 <212> DNA <213> Oscillibacter valericigenes <400> 180 atgaacaata tttacacgat gggcatcgac gtggggtcca ccgcatccaa gtgcctcatc 60 ctgaaagacg gcagcgaaat cgttgccaag tctctggtag atgtgggcgc gggtaccagc 120 ggccctaccc gtgctattgc ggaggtactg gaagccgcgg ggatgaagaa ggaggacatg 180 gcttttattc tggctaccgg ctatggccgc aattcactgg acgacattgc cgaccaccag 240 atgagcgagc tgagctgcca tgccaaaggc gcgtttttcc tgtttccgga tgtccacacc 300 gtcatcgaca tcggcgggca ggatgtgaag attcttgaga ttgagaacgg cgttatggtg 360 aattttgcca tgaatgacaa gtgcgccgcc gggacgggcc ggttcctgga cgtgatggcc 420 cgggtgctgg aggtgaaggt ggaggatctg gcggacctgg gagcccagtc caccaagaat 480 gtggagatca gctccacatg caccgtgttc gctgagagcg aggtcatcag ccagctggcc 540 aagggcagcg acaagcgcga catcatccac ggcatccaca agtctgtggc atcccgggtg 600 gttggccttg ccaaccgtat cggtgtgcgg gacgcggtgg tgatgaccgg cggcgtcgcc 660 cagaacggcg gcgtggtctc cgcgcttcag gaggcgttgg gccatcccat tcacacttcg 720 cctctgacgc agtacaacgg cgcgctgggc gcggcgttgt ttgcatggca gaaggcaacc 780 aaataa 786 <210> 181 <211> 1284 <212> DNA <213> Oscillibacter valericigenes <400> 181 atggccgaaa acgaaaaagc cactgcggcc gctcccgagg cggctcctgt taagaaagct 60 ccgaagccgg tcagccccgg tacgcaggcg ctgcgcgacg ttgtcaccaa ggtgtacgcc 120 gccgcgtggg atgcgaaaaa ggcgggccgc cccgtgggct ggtcgtcttc caagttcccc 180 tgcgagatcg ccgaggcgct gggccttgca gtcgtatatc cggaaaacca ggctgccggt 240 atcggcgccc agcacgatgg ccagcggatg tgtgaatctg ccgagtcctt gggcttcgac 300 ccagatatct gcggatacgc ccggatttcc ctggcttatt ccgcgggcgt tgagacgacc 360 aatgagtccc gccgggttcc catgccggac ttcgtgctgt gctgcaacaa tatttgtaac 420 tgcatgacca agtggtatga gaatattgcc cggatgcaca acattcccct gattatgatc 480 gacgtgccct ataacaacga ggtcaccgtc agcgattccc aggtggctta cattcgcggc 540 cagttcgatg acgccattaa gcagatggag aagattgccg gcgtgaagtt cgacgaaaag 600 aagtttgaac aggcctgcgc caatgccaac cgcactgcca aggcgtggct gacggtctgt 660 gactatttgc agtataagcc cgctcccatg agcggcttcg atctgtttaa ccatatggct 720 gatgtggtga ctgcccgcgg caaggtggag actgccgagg cgttcgagct gctggcaagc 780 gagctggaac agcacgtaaa aaacggaacc agcaccgctc cgttccccga gcagtaccgc 840 gtcatgttcg agggcattcc ctgctggccc aacctaagga cgcttttcaa gcccctgaaa 900 gccaacggcg tcaacgtcac cgccgtggtg tacgcgcccg cgttcggttt tgtgtataac 960 gggctggacg agatggcccg cgcatactgc aaggccccca acagcgtgtg cattgagcag 1020 ggcgtggact ggcgcgaggg catctgtcgc gagaacaagg tagacggcgt gctggtgcac 1080 tataaccgat cctgcaagcc ctggtccggc tacatggccg agatgcagcg ccgtttcacc 1140 aaggatctgg gcgtcccctg cgccgggttc gacggagatc aggccgatcc ccgcaacttc 1200 aacgaggctc agtatgagac ccgtgtccag ggcctggtag aggctatgga ggagaataaa 1260 aagcagaagg aggcccgggc atga 1284 <210> 182 <211> 1143 <212> DNA <213> Oscillibacter valericigenes <400> 182 atgagtatcg aaacgattgt aaaggagttt gccgacgttg cggccgaccc gaaagcacag 60 ctgaagaaat acaaggcgga gggcaaaaaa tgcattggtg tgatgccgta ttacgcgccc 120 gaggagctgg tggccgccgc cggtatggtg ccgtttggta tgtggggcag caatgacaag 180 accatttctc gcgccaagga atactgcgct acattttact gcaccatcgc ccagctggat 240 cttgagatgc tgctggacgg caccatggat cttttagacg gagtcatcac ccccaccatc 300 tgcgacacgc tccgtcccat gagccagaac atccgcgtgg ccatgggcga gaagctcccc 360 tgcattttcc tggcccatcc ccagaaccgc aagcccgctt acggcaagaa gttctgcctg 420 gaccaatata cccacatcaa gactgagctt gagaagatcg ccggcgcgcc catcaccgac 480 gccgcactgt ccgagaccat caaggtctat aataagagcc gcgccgcccg ccgtgagttc 540 gtgaagctgg tcagcgacca ctgcgatgtt atcaccccca ccaaacgcag cgctgttttg 600 aaagccgcgt ggtttatgcc caaggcggag tacaccgaga agctgaaggc cctcaacgca 660 gagctgaagg ctctgcctgt gtgcgactgg aaggggacca aggtggtcac ctccggcatc 720 atatgcgaca accctaagct tctggagatc ttcgaggaga acaaaatcgc catcgccgcc 780 gacgacgtgg ctcatgagtc ccgctccttc cgcgtagacg ctcccgagac cggcgatccc 840 atggaggcac tcgcccagca gtttgccaat caggattacg atgttctgct gtacgatgag 900 cattccagcg agaaccgccg gggcgagttt gtggccaagc tggtgaagga cagcggcgcc 960 aaggggctgg tcctgtttat gcagcagttc tgcgacccgg aggagatgga gtatccctcc 1020 ctcaaaaagg cgctggacga agccaagatc ccccacatca agctgggtgt ggatcaacag 1080 atgcgggact tcggtcaggc tcgcaccgcg attcaggcgt ttgccgatgt gatctccctc 1140 taa 1143 <210> 183 <211> 1269 <212> DNA <213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) <400> 183 atgactgata cagccaatat gagtgctaaa gaattgttag gtttctatca ggaagaattg 60 tatgaagaag cgagacaggc caaaaaagaa ggaaaacttg tttgctggtc ggcttccgtt 120 gctccttcgg agttttgtgt agctatggac gtggccatga tctatcctga aacccatgct 180 gcagggatcg gggccagaaa aggcgcctta gatatgcttg aagttgccga tgaaaaaggg 240 tataacctgg acacttgctc ctatgccaga gtgaatctgg gttatatgga acttttaaaa 300 caagaggctt taaccggaat aaccccggag aaactggaaa aatctccggc ggccagagta 360 cccctgcctg attttgtcat aacctgcaac aacatttgta acaccttgct taagtggtat 420 gaaaatcttg ccgttgagct aaatattccc tgcatcgtca ttgatgttcc ctttaatcac 480 accatgccca ttccccagta tgctaaagac tatattgcgg aacagtttaa ggaggcaatt 540 gctcagcttg aagagatttg cggcaagaaa ttcgactatg acaaattctt gcaagtccag 600 gaacaaaccc agcgctctgt ggcccaatgg aaccggattg cttctttgtc agggcataaa 660 ccatccccct taaatggttt tgatcttttc aactatatgg ccctgatcgt ttgtgcccgc 720 agcagggact gcgcagaaat tacctttaaa aagtttgccg atgaactgga agacaatcta 780 agcaaaggaa tctacgcctt taaaggcaat gaacaaaagc gtatcacttg ggaaggcatc 840 gctgtttggc cgcacctggg ccataccttt aaaggcttaa agaatcttgg caatatcatg 900 accggttcag cctatcccgg tttgtggaat ctttcttata cgcccggtga tatgagttcc 960 atggcagaag cttacaccag aatttatatc aatacttgtc tggataacaa agttaaggtt 1020 cttagtgaca tcatcagcgg cggaaagtgt gacggtgtta tttatcatca gaacagaagc 1080 tgtaagctca tgagttttct caatgtcgaa acggccgata tcctccaaca acaaaatcat 1140 ttaccctatg tcagctttga tggagaccaa accgatcccc gtaactttgc tcctgcccag 1200 tttgatacac ggatccaagc cttagatgaa atgatgaagc agaataagga gggagtttcc 1260 catgagtag 1269 <210> 184 <211> 1119 <212> DNA <213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) <400> 184 atgagtagaa ttgaagcgat tatcagtgaa ttatcttcta ttgccaataa tccccgtaag 60 gccatggaag attataagaa agaaacgggc aaagggtcgg tagggattat gccttattat 120 gctccggaag aaatcgttca tgccgccggt tacctgcccg taggaatttg gggagggcaa 180 aagagtattt ctaaagcccg tgcttattta cctccttttg cttgttcaat catgcaatcc 240 gttgtggaaa tgcagctgga aggggtctat aacgacttag cggcggtcct tttccccgtt 300 ccttgtgaca ctttaaaatg tctcagccaa aaatggaaag gcacatcccc ggtcatcgtc 360 atgactcatc ctcaaaaccg aaaactcgaa gcagccaata agtttctggc tgaggaatat 420 cgccttgttc gtgaaaagct ggaaaaaatc ttaaatgttc agattaccga tgaggcactg 480 aaccacagca ttgatgttta taacgaaaat cgcaaggcaa tgcgtgaatt tacggacata 540 gccgctaatt atttgaacat tattgatccc agaaagcgtc atgagattat caaggccaga 600 ttctttatgg aaaaatccaa acataccgcc ttggtcaaag aattgaattc cgagcttaaa 660 tctttacctg tggaagattt tacaggcaaa aaggtgattt taaccggaat catggctgaa 720 cccaatgaag tattagacat tttgaaagag aatgattttg ctgttgtggc agatgacctg 780 gcccaggaat ccagactgtt caggattgat gttccggctg gtccagaccc actctaccgc 840 ttggctaaat ggtggcaaga attcgacggt tgttctctgg ctgtagatac gaaaaaatta 900 agaggaccca tgctgatgaa tatggttaac gtggataagg ccgatgccgt ggtggtttgc 960 atgatgaagt tctgtgaccc tgaagaattt gactatccca tctactacag acagtttgaa 1020 gaagccggaa ttaagagctt atttatagaa attgacctgg agccaacctc ctttgaacag 1080 actaaaacca gagttcaaag ttttcgtgaa atgctgtga 1119 <210> 185 <211> 801 <212> DNA <213> Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628) <400> 185 atgtatacta tggggattga tatcggttcc tcatcctcaa aggttgtcat acttgaagat 60 ggagttaacc tcatcgccgg cgaagtcatt cagattggaa caggctcgac aggtcctaaa 120 cgggtactgg aggaagctct tgccaaaaca ggtctcacct tggcagacat ggctaaaatt 180 attgctaccg gctacggccg atcttctgtg gaagtatccg acaagcaaat cagcgaaatc 240 agctgtcagg ctaagggagt ttacttttta gttcctacag caaaattaat cattgatatc 300 ggcggtcagg atgtgaaggc cattagactt gaccgtatag gcggcgtcag gcagtttttt 360 atgaatgata aatgtgccgc cggaacagga cgttttctcg atgtgatgtc acgagtactg 420 gaagtggatc tggatgaaat ggcagaatac gatgcccggg ccacagaacc cgccacggtc 480 agcagcacct gcacagtgtt tgccgaatcc gaggtaatat ctcagcttgc caacggagtt 540 gctaaagaga atattattgc cggggttcac cagtccgttg ccagcaaagc ctgtggactc 600 gcctatcgat gcggggtgga agaggacgtt gtgatgtgcg gaggagttgc taaggactta 660 ggagttgtcc gggccatcag caaagaacta aaaaaaccgg tcattgtagc tcctaatccc 720 caaattacag ccgcccttgg cgctgcccta tttgcttatg aagaagttat ggaagctaat 780 aaattaagga aagaggtatg a 801 <210> 186 <211> 1236 <212> DNA <213> Peptostreptococcus anaerobius CAG:621 <400> 186 atgagtaaca caggtgcagt tgaagaaaag ccggcaaaag tattgttagg cgagatagtt 60 gcaaaacatt ataaggaagc ttgggaagct aaagaaagag gcgaaaaagt tggttggtgt 120 gcttctaact tcccacagga aatatttgaa acaatggata tcaaggttgt attccctgaa 180 aaccaggcag cagcaatttc tgctaagggt ggtggacaga ggatgtgcga aatcgcagaa 240 aacgaaggat attcaaacga catatgtgct tacgctagaa tatctctagc atacatggac 300 gttaaagatg ctccagagtt aaatatgcct cagccagact ttgttgcatg ctgtaacaat 360 atctgtaact gtatgatcaa gtggtatgaa aatatagcta aagaactaaa tatacctcta 420 atccttgttg acgtgccata taacaatgac tatgaagcag gcgatgacag agtagaatac 480 ttaagaggac agttcgatca cgctataaag cagttagaag acttaactgg taaaaagtgg 540 gatgaaaaga agttcgaaga agtaatggca atatctcaga gaacaggtag agcttggtta 600 aaggctactg gatatgctaa gtacactcca tcaccattct caggatttga cgtattcaac 660 catatggcag ttgctgtatg tgctagaggt aaggaagaat cagcaatagc atttgaaaag 720 ctagctgaag aatttgatga aaatgtaaag actggtaagt ctacattcaa gggagaagaa 780 aagtacagag tactatttga aggtatagct tgttggccac acctaagaca tacatttaag 840 cagctaaagg attcaggagt aaacgtttgt ggtactgttt atgcagatgc attcggatac 900 atctacgaca atacttatga attaatgcag gcttattgtg gaactcctaa tgcaatatct 960 tatgaaagat cattagatat gagacttaag gttatagaag aaaataatat agacggtatg 1020 ttgatacata taaacagaag ctgtaagcag tggtctggta tcatgtacga aatggaaaga 1080 gaaataagag aaagaactgg tataccaaca gctacattcg atggtgatca ggctgaccca 1140 agaaacttct cagaagcaca gtacgacaca agagtacagg gtctaataga agttatggaa 1200 gcaaacaaag ctgcaaagat gaaggaggaa aactag 1236 <210> 187 <211> 1119 <212> DNA <213> Megasphaera elsdenii DSM 20460 <400> 187 atgagtcaga tcgacgaact tatcagcaaa ttacaggaag tatccaacca tccccagaag 60 acggttttga attataaaaa acagggtaaa ggcctcgtag gcatgatgcc ctactacgct 120 ccggaagaaa tcgtatatgc tgcaggctac ctcccggtag gcatgttcgg ttcccagaac 180 ccgcagatct ccgcagctcg tacgtacctt cctccgttcg cttgctcctt gatgcaggct 240 gacatggaac tccagctcaa cggcacctat gactgcctcg acgctgttat cttctccgtt 300 ccttgcgaca ctctccgctg catgagccag aaatggcacg gcaaagctcc ggtcatcgtc 360 ttcacacagc cgcagaaccg taagatccgc ccggctgtcg atttcctcaa agctgaatac 420 gaacatgtcc gtacggaatt ggaacgtatc ctcaacgtaa aaatctccga cctggctatc 480 caggaagcta tcaaagtata taacgaaaac cgtcaggtta tgcgtgaatt ctgcgacgta 540 gctgctcagt acccgcagat cttcactccg gtaaaacgtc atgacgtcat caaagcccgc 600 tggttcatgg acaaagctga acacaccgct ttggtccgcg aactcatcga cgctgtcaag 660 aaagaaccgg tacagccgtg gaatggcaaa aaagtcatcc tctccggtat catggcagaa 720 ccggatgaat tcctcgatat cttcagcgaa ttcaacatcg ctgtcgtcgc tgacgacctc 780 gctcaggaat cccgccagtt ccgtacagac gtaccgtccg gcatcgatcc cctcgaacag 840 ctcgctcagc agtggcagga cttcgatggc tgcccgctcg ctttgaacga agacaaaccg 900 cgtggccaga tgctcatcga catgactaag aaatacaatg ctgacgccgt cgtcatctgc 960 atgatgcgtt tctgcgatcc tgaagaattc gactatccga tttacaaacc ggaatttgaa 1020 gctgctggcg ttcgttacac ggtcctcgac ctcgacatcg aatctccgtc cctcgaacag 1080 ctccgcaccc gtatccaggc tttctcggaa atcctctaa 1119 <210> 188 <211> 1119 <212> DNA <213> Peptostreptococcus anaerobius CAG:621 <400> 188 atgagtaact tagaagaact atttggaaaa cttgctgtat gtccattaga gcagatagat 60 aaatatgttg ctgatggtaa gaaagttatt ggttgcgcgc cagtatatgc tccagaagaa 120 cttgtatacg catcaggtat gattcctatg gcaatatggg gagcagaggg tgaagtaact 180 cttgcaaaag aatatttccc agctttctac gtatcaatca tcttaagact tttagatcta 240 ggtctagaag gcaagcttga taagatgtca ggaatgattc taccaggtct aagtgacgga 300 ctaaagggac ttagccagaa ctggaaaaga gctgtaaaga atgttccagc attatatata 360 ggatatggac agaacagaaa gatagaagct ggtatagttt acaatgctag acagtatgaa 420 aagctaaaag tacagttaga agaaatagct ggaaagaaga tagaagatgc tcagatagaa 480 gaagcaatcg ttttatacaa caagcacaga aaagctatgc aggcattctc agaccttgca 540 gctaaacact taaatacagt tactcctagc ctaagagcta aggtaatgtc aagtgcatgc 600 ctaatggaca aggctgaaca tttagaaata gtagaagcaa tcaacgctga actttcagct 660 atgccagaag aaaaatttga tggtaagaag attgtaacta ctggactact agctaacagt 720 cctgaaatat taaagatatt tgaagaattt aaacttggta ttgttgctga caacataaac 780 cacgaatcag gacagtttga ttatttagtt gatgaagcta ctggtaaccc aataaaggcg 840 ttgtctaagt ggatttcaga tattgaagga agtactttgc tatacgatcc agaaaaacta 900 agaggacaga taatcatcga taaggctaaa aaatacgatg cagatggtgt agtataccta 960 ctatctaaat tctctgattc agatgaattt gactacccaa tcattagaaa acagctagaa 1020 gaggctggat atatgcacat cttagttgaa gtagatcagc aaatgactaa cttcgaacaa 1080 gcaaaaactg cattgcagac ttttgcagac atgatatag 1119 <210> 189 <211> 792 <212> DNA <213> Peptostreptococcus anaerobius CAG:621 <400> 189 atgagtgata tatacacaat gggtattgac attggatcaa catcatctaa atgtgtagtg 60 cttaagaatg gtaaagattt agttagtagc ggcgtcgtca atcttggcgc cggtactaaa 120 ggtgccgatc aggttataga aaaggtacta gctgactgtg gtatcaagtt cgaagatctg 180 aatgtgattg tttccacagg atatggtaga aattcttacg acagtgcaaa gaagactatg 240 agtgaactta gctgtcatgc taagggtggt acatatatct tcggacctgt aagaactatt 300 atagatatag gcggacagga cataaaggta ctaaaactaa atgacaaagg tatgatgaca 360 aatttcttga tgaatgataa atgtgcagct ggtacaggta gattcttaga ggttatggct 420 ggagtacttg atgttaagct agcagaacta ggtgacttag acaagttagc aactgaaaaa 480 acaccaatat cttcaacttg tacagtattt gcagaatcag aagtaatatc ttgtatggct 540 aagaaaatac ctattcctaa tataattagg ggtatacacg cttctgttgc tacaagagtt 600 gcaggtcttg ctaagagagg tggattaaca actccagtcg ctatgactgg tggtgttact 660 aagaactcag gaatagtaag ggcacttagc gaagagttag aaacagatat catgatttcg 720 gaaatttctc agttggcagg cgcaattgga gcggcattgt acgcttacga tgagtatctg 780 aaggaaaatt ag 792 <210> 190 <211> 777 <212> DNA <213> Chloroflexus aggregans (strain MD-66 / DSM 9485) <400> 190 atgagcgatg aaacgcttgt gctcagcact atcgaaggcc ccgttgcaat ccttacgctc 60 aatcgaccac aagcactcaa tgcccttagc cctgccctca tcgacgcact catccgccat 120 cttgagcatt gcgataacga cgatacgatc cgggtgatca ttatcaccgg cgccggtcgc 180 gcctttgccg ccggcgccga catcaaggcg atggccgatg cgacgccgat cgatatgctt 240 acaaccgata tgattgcccg ctgggcgcgg attgcggcgg tgcgcaaacc cgtgatcgca 300 gccgtgaacg gatttgccct cggtggtggc tgcgagttgg ctatgatgtg tgacatcatt 360 cttgccagtg aaacagccca attcggtcaa cccgaaatca acatcggcat tatccccggc 420 gccggtggca cccaacgcct gacccgcgca attggcccat accgtgcaat ggagatggtc 480 ttaaccggtg ctaccatcag tgcccaagaa gcttacgcct acggcctggt gaatcgggta 540 tgcccacccg atagcctgct tgatgaagcc cgccggttgg cccagaccat tgcagccaag 600 ccgccgctcg ctgtgcgttt agccaaggaa gccgtgcgcg ctgcggctga aacgaccgtg 660 cgtgaagggt tagccattga attgcgtaac ttttatctgc tctttgccag tgccgatcag 720 aaagagggca tgcgagcctt tatcgaaaag cgtacagcca acttcagtgg tcgctaa 777 <210> 191 <211> 774 <212> DNA <213> Marivirga tractuosa (strain ATCC 23168/DSM 4126/NBRC 15989/NCIMB 1408/VKM B-1430/H-43) <400> 191 atggaattca taaaagtaaa cacacaatat aaaaagcata ttgcgctcat caatcttaac 60 agacctaaag aattaaatgc cttgaactta cagttaatga ctgaattgaa ggacacttta 120 aaggtcttgg atgaggatga aaatgttaga gttataattt taacaggtaa tgagaaggct 180 tttgccgctg gagcagacat taagcaaatg gcaggtaaaa cggctattga catgctcaat 240 gttgatcaat tcagcacttg ggatcaaatc aaaaaaacaa agaagccatt gattgcagcc 300 gtttcaggat ttgcattggg cggtggttgc gaattagcga tgacttgcga tatgattgta 360 gcgtcagaat ctgctaaatt cggtcagcct gaaataaaaa tcggagtaat gccgggagca 420 ggtggtacac aaaggttaac tagggcaatt ggtaaagcca aagcgatgga attagtcttg 480 actggtaatt ttattagtgc agaggaagca atgcattatg gcttagttaa taaagttgtt 540 cctacagaga tgtatctgga agcagctgct gaactggctg agcaaatagc acaaatgtct 600 cctgtagcag ctaagttggc aaaagaatca gttaacaggg cttttgaaac gcatttggac 660 gaaggcttgc actttgagag aaaaaacttc tatttaacat ttgcttcaga agatcagact 720 gaaggtatgg aagcttttgt agagaaaaga aagcctgaat tcaaggggaa ataa 774 <210> 192 <211> 774 <212> DNA <213> Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) <400> 192 atgtacgaga acctcatcgt ggagacgctc gagggcggcg tggggctcat tcgcatccac 60 cggcccaagc gcctcaacgc cctgaaccag gccaccatgg acgagatcgt ccgcgcagta 120 cgcgcgtttg aagcggatga cgcggtgcgc gcgatcgtcc tcacggggga cgagcgggcg 180 ttcgccgcgg gcgcggacgt caccgagatg gacggcgcga acgtgccgga gatgctctcc 240 gggtaccgct tcgagcagtg ggagaccctc cggcgcacca cgaaaccctt gatcgccgcg 300 gtctcggggt tcgcgctcgg gggcgggctc gagctcgcga tgctgtgcga catcatcgta 360 gcctcggaga ccgcgcggct cggccagccc gagatcaacc tcgggatcat gccgggggcg 420 ggcggcacgc aacggctcac gcggcaggtg ggcaagtacc tcgcgatgga gatggtcctc 480 acggggcgca tgctcaccgc ggaggaggcg taccgtcacg gcctggtgaa ccgggtcgtc 540 ccggtcgagt tctacctgga ggaagccatc cagatcgcgc gggagatcgc gaagaaagcc 600 ccggtggcgg tgcgcctggc caaggacgcg atcctcaagg cagaggacac gccgctcgag 660 gtgggcctcg cgtacgagcg ccacaacttc tacctgctct tcggcaccga ggacaagcaa 720 gaagggatcc gcgctttcct cgagaagcgc aagcccgaat ggaaagggag gtag 774 <210> 193 <211> 780 <212> DNA <213> Chitinophaga pinensis (strain ATCC 43595 / DSM 2588 / NCIB 11800 / UQM 2034) <400> 193 atgcaaccac aatttataat catacaccgg caggtagccc catatgtggc tcatatacag 60 ttaaaccgcc ccaaagaact caatgcactg aaccttgaac tgatgattga gctcagggat 120 gcattaaaaa tgttggatgc ggatgacaat gttcgtgcaa tcgtcatcag cggtaatgaa 180 aaagcattcg ctgcaggcgc ggatatcaaa cagatggcgg ggaaaactgc catggacatg 240 tataacattg accagttcag cacctgggac acaataaaaa aaactaaaaa gccgttgatt 300 gcggcagtaa gcggcttcgc gctgggaggg ggatgtgagc tggtgatgct atgcgatatg 360 atagtagcca gtgaaacagc gcggttcgga cagccggaaa taaaaattgg cgtcatgcct 420 ggcgcaggtg gtacacaacg cctgacccgc gccgtaggta aagccctggc catggaaatg 480 gtattgacag gtcgctttat cactgcacaa gaagctgcac gtgcaggtct tatcaaccgg 540 gtaataccgg tggaactttt cctgcaggaa gccatccggc tggcgactga agtagctgcg 600 cttagtccgt tggcagtaaa gatggctaaa gaatctgtac tgaaagcatt tgatagctcc 660 ctcgaagaag gactacattt tgaacgtaaa aacttttatc tgctgtttgc ctctgaagat 720 cagaaagaag gcatgcaggc ttttgttgat aagagatcac ctgtttttaa aggaaaataa 780 780 <210> 194 <211> 777 <212> DNA <213> Megasphaera elsdenii DSM 20460 <400> 194 gtgtatactc tcggaatcga cgttggttct tcttcttcca aggcagtcat cctggaagat 60 ggcaagaaga tcgtcgccca tgccgtcgtt gaaatcggca ccggttcgac cggtccggaa 120 cgcgtcctgg acgaagtctt caaagatacc aacttaaaaa ttgaagacat ggcgaacatc 180 atcgccacag gctatggccg tttcaatgtc gactgcgcca aaggcgaagt cagcgaaatc 240 acgtgccatg ccaaaggggc cctctttgaa tgccccggta cgacgaccat cctcgatatc 300 ggcggtcagg acgtcaagtc catcaaattg aatggccagg gcctggtcat gcagtttgcc 360 atgaacgaca aatgcgccgc tggtacgggc cgtttcctcg acgtcatgtc gaaggtactg 420 gaaatcccca tgtctgaaat gggggactgg tacttcaaat cgaagcatcc cgctgccgtc 480 agcagtacct gcacggtttt tgctgaatcg gaagtcattt cccttctttc caagaatgtc 540 ccgaaagaag atatcgtagc cggtgtccat cagtccatcg ccgccaaagc ctgcgctctc 600 gtgcgccgcg tcggtgtcgg tgaagacctg accatgaccg gcggtggctc ccgcgatccc 660 ggcgtcgtcg atgccgtatc gaaagaatta ggtattcctg tcagagtcgc tctgcatccc 720 caagcggtgg gtgctctcgg agctgctttg attgcttatg ataaaatcaa gaaataa 777 <210> 195 <211> 1287 <212> DNA <213> Megasphaera elsdenii DSM 20460 <400> 195 atgagtgaag aaaaaacagt agatattgaa agcatgagct ccaaggaagc ccttggttac 60 ttcttgccga aagtcgatga agacgcacgt aaagcgaaaa aagaaggccg cctcgtttgc 120 tggtccgctt ctgtcgctcc tccggaattc tgcacggcta tggacatcgc catcgtctat 180 ccggaaactc acgcagctgg tatcggtgcc cgtcacggtg ctccggccat gctcgaagtt 240 gctgaaaaca aaggttacaa ccaggacatc tgttcctact gccgcgtcaa catgggctac 300 atggaactcc tcaaacagca ggctctgaca ggcgaaacgc cggaagtcct caaaaactcc 360 ccggcttctc cgattcccct tccggatgtt gtcctcactt gcaacaacat ctgcaatacc 420 ttgctcaaat ggtatgaaaa cttggctaaa gaattgaacg tacctctcat caacatcgac 480 gtaccgttca accatgaatt ccctgttacg aaacacgcta aacagtacat cgtcggcgaa 540 ttcaaacatg ctatcaaaca gctcgaagac ctttgcggcc gtcccttcga ctatgacaaa 600 ttcttcgaag tacagaaaca gacacagcgc tccatcgctg cctggaacaa aatcgctacg 660 tacttccagt acaaaccgtc gccgctcaac ggcttcgacc tcttcaacta catgggcctc 720 gccgttgctg cccgctcctt gaactactcg gaaatcacgt tcaacaaatt cctcaaagaa 780 ttggacgaaa aagtagctaa taagaaatgg gctttcggtg aaaacgaaaa atcccgtgtt 840 acttgggaag gtatcgctgt ctggatcgct ctcggccaca ccttcaaaga actcaaaggt 900 cagggcgctc tcatgactgg ttccgcttat cctggcatgt gggacgtttc ctacgaaccg 960 ggcgacctcg aatccatggc agaagcttat tcccgtacat acatcaactg ctgcctcgaa 1020 cagcgcggtg ctgttcttga aaaagttgtc cgcgatggca aatgcgacgg cttgatcatg 1080 caccagaacc gttcctgcaa gaacatgagc ctcctcaaca acgaaggcgg ccagcgcatc 1140 cagaagaacc tcggcgtacc gtacgtcatc ttcgacggcg accagaccga tgctcgtaac 1200 ttctcggaag cacagttcga tacccgcgta gaagctttgg cagaaatgat ggcagacaaa 1260 aaagccaatg aaggaggaaa ccactaa 1287 <210> 196 <211> 1119 <212> DNA <213> Megasphaera elsdenii DSM 20460 <400> 196 atgagtcaga tcgacgaact tatcagcaaa ttacaggaag tatccaacca tccccagaag 60 acggttttga attataaaaa acagggtaaa ggcctcgtag gcatgatgcc ctactacgct 120 ccggaagaaa tcgtatatgc tgcaggctac ctcccggtag gcatgttcgg ttcccagaac 180 ccgcagatct ccgcagctcg tacgtacctt cctccgttcg cttgctcctt gatgcaggct 240 gacatggaac tccagctcaa cggcacctat gactgcctcg acgctgttat cttctccgtt 300 ccttgcgaca ctctccgctg catgagccag aaatggcacg gcaaagctcc ggtcatcgtc 360 ttcacacagc cgcagaaccg taagatccgc ccggctgtcg atttcctcaa agctgaatac 420 gaacatgtcc gtacggaatt ggaacgtatc ctcaacgtaa aaatctccga cctggctatc 480 caggaagcta tcaaagtata taacgaaaac cgtcaggtta tgcgtgaatt ctgcgacgta 540 gctgctcagt acccgcagat cttcactccg gtaaaacgtc atgacgtcat caaagcccgc 600 tggttcatgg acaaagctga acacaccgct ttggtccgcg aactcatcga cgctgtcaag 660 aaagaaccgg tacagccgtg gaatggcaaa aaagtcatcc tctccggtat catggcagaa 720 ccggatgaat tcctcgatat cttcagcgaa ttcaacatcg ctgtcgtcgc tgacgacctc 780 gctcaggaat cccgccagtt ccgtacagac gtaccgtccg gcatcgatcc cctcgaacag 840 ctcgctcagc agtggcagga cttcgatggc tgcccgctcg ctttgaacga agacaaaccg 900 cgtggccaga tgctcatcga catgactaag aaatacaatg ctgacgccgt cgtcatctgc 960 atgatgcgtt tctgcgatcc tgaagaattc gactatccga tttacaaacc ggaatttgaa 1020 gctgctggcg ttcgttacac ggtcctcgac ctcgacatcg aatctccgtc cctcgaacag 1080 ctccgcaccc gtatccaggc tttctcggaa atcctctaa 1119 <210> 197 <211> 777 <212> DNA <213> Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) <400> 197 atgagtgaag agtctctggt tctcagcaca attgaaggcc ccatcgccat cctcaccctc 60 aatcgccccc aggccctcaa tgcgctcagt ccggccttga ttgatgacct cattcgccat 120 ttagaagcct gcgatgccga tgacacaatc cgcgtgatca ttatcaccgg cgccggacgg 180 gcatttgctg ccggcgctga catcaaagcg atggccaatg ccacgcctat tgatatgctc 240 accagtggca tgattgcgcg ctgggcacgc atcgccgcgg tgcgcaaacc ggtgattgct 300 gccgtgaatg ggtatgcgct cggtggtggt tgtgaattgg caatgatgtg cgacatcatc 360 atcgccagtg aaaacgcgca gttcggacaa ccggaaatca atctgggcat cattcccggt 420 gctggtggca cccaacggct gacccgcgcc cttggcccgt atcgcgcaat ggaattgatc 480 ctgaccggcg cgaccatcag tgctcaggaa gctctcgccc acggcctggt gtgccgggtc 540 tgcccgcctg aaagcctgct cgatgaagcc cgtcggatcg cgcaaaccat tgccaccaaa 600 tcaccactgg ctgtacagtt ggcgaaagag gcagtccgta tggccgccga aaccactgtg 660 cgcgaggggt tggctatcga gctgcgtaac ttctatctgc tgtttgccag tgctgaccaa 720 aaagagggga tgcaggcatt tatcgagaaa cgcgctccca acttcagtgg tcgttga 777 <210> 198 <211> 777 <212> DNA <213> Ruegeria pomeroyi DSS-3 <400> 198 atggcctttg agacgatcat cgtcgaagtt gaagaccacg tagccctgat caggctgaac 60 cgtcccgatg cgctcaatgc gctcaacacc cagttgctgg gcgagttgtg taccgcgctg 120 gaagaggccg acggcaatga caaggtgcgc tgcatcgtca tcaccggcag cgacaaggca 180 tttgccgccg gggccgatat ccgcgagatg tcccaaaaga cctatgtcga ggtgtatagc 240 gagaacctgt tcgcggccgc caacgaccgt gtcagcgcca tccgcaagcc gatcatcgcc 300 gcagtggcgg gctatgcgct gggcggtggc tgtgaactgg cgatgctgtg cgatttcatc 360 atcgcggcgg acaccgcaaa gttcggccag cccgagatca acctgggcgt gatcgccggt 420 atcggcggca cccagcgtct gacccggctg gtgggcaagt ccaagtcgat ggacctgaac 480 ctgaccgggc ggttcatgga tgccgaagag gccgagcgcg ccgggctggt cagccgcgtg 540 gttccggcca agaagctggt cgaagaggcg ctgagcgcag cccagaagat cgccgagaaa 600 tcgatgatct cggcctatgc ggtcaaggag gcggtcaacc gctcttacga gaccacgctg 660 agcgaggggc tgctgttcga gcgccgggtg ttccattcga tgttcgccac cgaagatcag 720 aaggaaggca tggccgcttt cctcgagaag cgggcggcac agttccgcga caagtga 777 <210> 199 <211> 132 <212> PRT <213> E. coli <400> 199 Met Ser Thr Thr His Asn Val Pro Gln Gly Asp Leu Val Leu Arg Thr 1 5 10 15 Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly 20 25 30 Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu 35 40 45 Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Thr Phe 50 55 60 Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys 65 70 75 80 Val Gln Lys Gly Thr Thr Ser Val Ser Ile Asn Ile Glu Val Trp Val 85 90 95 Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu 100 105 110 Ala Leu Phe Lys Tyr Val Ala Val Asp Pro Glu Gly Lys Pro Arg Ala 115 120 125 Leu Pro Val Glu 130 <210> 200 <211> 132 <212> PRT <213> Klebsiella oxytoca 10-5245 <400> 200 Met Thr Thr Thr Asp Leu Ala Pro Lys Gly Glu Leu Val Leu Arg Thr 1 5 10 15 Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly 20 25 30 Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Met Ala Lys Glu 35 40 45 Ile Ala His Gly Arg Val Val Thr Val Arg Val Asp Gly Met Thr Phe 50 55 60 Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Asn Cys 65 70 75 80 Val Lys Arg Gly Asn Thr Ser Ile Thr Ile Asn Met Glu Val Trp Val 85 90 95 Lys Lys Val Ser Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu 100 105 110 Ala Leu Phe Ile Tyr Val Ala Val Asp Asn Gln Gly Lys Pro Arg Ala 115 120 125 Leu Pro Thr Leu 130 <210> 201 <211> 133 <212> PRT <213> Cronobacter turicensis <400> 201 Met Thr Thr Glu Gln Thr Thr Pro Gln Gly Glu Leu Val Leu Arg Thr 1 5 10 15 Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly 20 25 30 Trp Leu Met Ala Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu 35 40 45 Ile Ala His Gly Arg Val Val Thr Val Arg Val Asp Gly Met Thr Phe 50 55 60 Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys 65 70 75 80 Val Lys Arg Gly Asn Thr Ser Val Thr Ile Asn Ile Glu Val Trp Val 85 90 95 Lys Lys Val Ser Ser Glu Pro Leu Gly Gln Arg Tyr Arg Ala Thr Glu 100 105 110 Ala Leu Phe Ile Tyr Val Ala Val Asp Asp Asn Gly Lys Pro Arg Pro 115 120 125 Leu Pro Pro Val Ala 130 <210> 202 <211> 133 <212> PRT <213> Citrobacter freundii <400> 202 Met Thr Thr Thr Asn Asn Thr Pro Gln Gly Glu Leu Val Leu Arg Thr 1 5 10 15 Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly 20 25 30 Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Gln Ala Lys Glu 35 40 45 Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Ser Phe 50 55 60 Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys 65 70 75 80 Val Lys Arg Gly Thr Thr Ser Ile Ser Ile Asn Ile Glu Val Trp Val 85 90 95 Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu 100 105 110 Ala Leu Phe Ile Tyr Val Ala Val Asp Lys Asp Gly Lys Pro Arg Pro 115 120 125 Ile Pro Thr Leu Ala 130 <210> 203 <211> 130 <212> PRT <213> Salmonella enterica <400> 203 Met Asp Asn Thr Pro Gln Gly Glu Leu Val Leu Arg Thr Leu Ala Met 1 5 10 15 Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly Trp Leu Met 20 25 30 Ser Gln Met Asp Ile Gly Gly Ala Ile Leu Ala Lys Glu Ile Ala His 35 40 45 Gly Arg Val Val Thr Val Arg Val Glu Gly Met Thr Phe Leu Arg Pro 50 55 60 Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys Val Lys Arg 65 70 75 80 Gly Thr Thr Ser Ile Ser Ile Asn Ile Glu Val Trp Val Lys Lys Val 85 90 95 Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu Ala Leu Phe 100 105 110 Ile Tyr Val Ala Val Asp Pro Asp Gly Lys Pro Arg Pro Leu Pro Val 115 120 125 Gln Gly 130 <210> 204 <211> 133 <212> PRT <213> Shigella flexneri 1235-66 <400> 204 Met Thr Thr Thr Asn Asn Thr Pro Gln Gly Glu Leu Val Leu Arg Thr 1 5 10 15 Leu Ala Met Pro Ala Asp Thr Asn Ala Asn Gly Asp Ile Phe Gly Gly 20 25 30 Trp Leu Met Ser Gln Met Asp Ile Gly Gly Ala Ile Gln Ala Lys Glu 35 40 45 Ile Ala His Gly Arg Val Val Thr Val Arg Val Glu Gly Met Ser Phe 50 55 60 Leu Arg Pro Val Ala Val Gly Asp Val Val Cys Cys Tyr Ala Arg Cys 65 70 75 80 Val Lys Arg Gly Thr Thr Ser Ile Ser Ile Asn Ile Glu Val Trp Val 85 90 95 Lys Lys Val Ala Ser Glu Pro Ile Gly Gln Arg Tyr Lys Ala Thr Glu 100 105 110 Ala Leu Phe Ile Tyr Val Ala Val Asp Lys Asp Gly Lys Pro Arg Pro 115 120 125 Ile Pro Lys Gln Val 130 <210> 205 <211> 399 <212> DNA <213> E. coli <400> 205 atgtctacaa cacataacgt ccctcagggc gatcttgttt tacgtacttt agccatgccc 60 gccgatacca atgccaatgg tgacatcttt ggtggttggt taatgtcaca aatggatatt 120 ggcggcgcta ttctggcaaa agaaattgcc cacggtcgcg tagtgactgt gcgggttgaa 180 ggaatgactt tcttacggcc ggttgcggtc ggcgatgtgg tgtgctgcta tgcacgctgt 240 gtccagaaag ggacgacatc ggtcagcatt aatattgaag tgtgggtgaa aaaagtagcg 300 tctgaaccaa ttgggcaacg ctataaagcg acagaagcat tatttaagta tgtcgcggtt 360 gatcctgaag gaaaacctcg cgccttacct gttgagtaa 399 <210> 206 <211> 399 <212> DNA <213> Klebsiella oxytoca 10-5245 <400> 206 atgacaacaa cagatcttgc gccgaagggc gaattggttt tacgcaccct ggcgatgccg 60 gcggacacca acgcaaacgg cgatattttc ggcggctggc tgatgtcgca aatggatatt 120 ggcggggcca ttatggccaa agaaattgcc cacggtcgcg tcgtgaccgt gcgcgtcgac 180 ggcatgacct ttttgcgccc ggtggcggtc ggcgacgtcg tgtgctgcta cgccaactgc 240 gtgaagcgcg gcaatacgtc gataactatc aatatggaag tgtgggtcaa gaaagtgtcg 300 tctgagccca tcggccagcg ctacaaagcc accgaagcgc tgtttatcta cgtcgcggtg 360 gataatcagg gaaaaccgcg cgcactgccg actctgtga 399 <210> 207 <211> 402 <212> DNA <213> Cronobacter turicensis <400> 207 atgacgacag agcaaaccac gcctcaaggt gaactggttt tacgtaccct ggcgatgccc 60 gccgatacca acgccaatgg cgatattttt ggcggctggc tgatggccca gatggacatt 120 ggcggcgcga tccttgccaa agagatagcc catggccgcg tggtgacggt acgcgttgac 180 ggcatgacgt tcctgcgccc ggtcgcggtt ggcgatgtgg tgtgctgtta tgcccgttgc 240 gtgaagcgcg gcaatacatc ggtgacgatt aatattgaag tgtgggtgaa gaaggtttct 300 tccgagccgc ttggccagcg ctaccgcgcg accgaggcgc tgttcattta tgttgcggtc 360 gatgacaacg gcaaaccgcg cccgctgccg cctgtggcgt ga 402 <210> 208 <211> 402 <212> DNA <213> Citrobacter freundii <400> 208 atgacaacaa cgaataacac tccccagggt gaactggttt tacgcactct ggccatgcct 60 gccgatacca acgcgaacgg tgatattttt ggcggctggc tgatgtcaca aatggatata 120 ggtggcgcga ttcaggccaa agagatcgca catggtcgtg tggtaactgt gcgggttgaa 180 ggaatgagct ttttgcgccc ggtcgccgta ggtgatgtag tgtgttgcta tgctcgctgt 240 gtgaaacgcg ggacaacctc aatcagcatc aatattgaag tttgggtgaa gaaagtcgct 300 tctgaaccta ttggccagcg ttataaggcc accgaagctc tgtttatcta cgttgccgtt 360 gataaagacg ggaaaccgcg tccaatcccc acgttggcct ga 402 <210> 209 <211> 393 <212> DNA <213> Salmonella enterica <400> 209 atggataata ctcctcaggg cgagctggtt ttacgtacat tggccatgcc tgccgatacc 60 aatgcgaacg gcgatatttt tggcggctgg ctgatgtcgc aaatggatat tggcggcgcg 120 atactggcca aagagatcgc gcacggtcgg gttgtaaccg tacgcgtgga aggaatgaca 180 tttctgcgcc ccgtcgcggt tggcgatgtc gtatgctgct acgcgcgctg cgttaaacgc 240 ggtacgacgt ctattagcat aaatattgaa gtctgggtga aaaaagtcgc gtcagaaccg 300 attgggcagc gctacaaggc caccgaggcg ctgtttattt atgttgccgt cgatccggac 360 ggtaaacctc gcccgctccc ggttcagggt taa 393 <210> 210 <211> 402 <212> DNA <213> Shigella flexneri 1235-66 <400> 210 atgacaacaa cgaataacac cccccagggt gaactggttt tacgcactct ggccatgcct 60 gccgatacca atgctaacgg tgatattttt ggcggctggc tgatgtcaca gatggatatt 120 ggtggcgcta ttcaggccaa agagatcgca cacggtcgcg tggtgacggt gcgagttgaa 180 ggaatgagct ttttgcgccc ggttgccgtg ggtgatgtgg tctgttgcta cgcacgctgc 240 gtaaaacgcg ggacgacgtc aatcagcatt aatattgaag tctgggtgaa gaaagtcgct 300 tcggaaccta ttggccagcg ttacaaagcc actgaagccc tgtttatcta cgtcgctgta 360 gataaagacg gtaaaccccg tccgatacct aaacaggtct ga 402 <210> 211 <211> 554 <212> PRT <213> Ilyobacter polytropus DhaB1 protein <400> 211 Met Lys Ser Lys Arg Phe Glu Val Leu Lys Glu Arg Pro Val Asn Lys 1 5 10 15 Asp Gly Phe Ile Ser Glu Trp Ile Glu Glu Gly Leu Ile Ala Met Glu 20 25 30 Ser Pro Asn Asp Pro Asn Pro Ser Leu Lys Ile Glu Asn Gly Gln Ile 35 40 45 Thr Glu Leu Asp Gly Lys Ser Arg Glu Glu Phe Asp Met Ile Asp Arg 50 55 60 Phe Ile Ala Asp Tyr Ala Ile Asn Met Glu Asn Ala Glu Lys Ala Met 65 70 75 80 Lys Met Ser Ser Met Glu Ile Ser Lys Lys Leu Val Asp Ile Asn Val 85 90 95 Ser Arg Asp Glu Val Leu Glu Ile Thr Thr Gly Ile Thr Pro Ala Lys 100 105 110 Ile Ile Lys Val Met Glu His Met Asn Val Val Glu Met Met Met Ala 115 120 125 Val Gln Lys Met Arg Ala Arg Lys Thr Pro Ser Asn Gln Cys His Val 130 135 140 Thr Asn Leu Arg Asp Asn Pro Val Leu Ile Ala Ala Asp Ala Ala Glu 145 150 155 160 Ala Ser Val Arg Gly Phe Asp Glu Gln Glu Thr Thr Ile Gly Ile Val 165 170 175 Arg Tyr Ala Pro Phe Asn Ala Ile Ser Ile Phe Val Gly Ser Gln Val 180 185 190 Gly Arg Gly Gly Ile Leu Thr Gln Cys Ser Val Glu Glu Ala Thr Glu 195 200 205 Leu Glu Leu Gly Met Lys Gly Phe Thr Ser Tyr Ala Glu Thr Val Ser 210 215 220 Val Tyr Gly Thr Glu Gln Val Phe Ile Asp Gly Asp Asp Thr Pro Trp 225 230 235 240 Ser Lys Ala Phe Leu Ala Ser Ala Tyr Ala Ser Arg Gly Leu Lys Met 245 250 255 Arg Phe Thr Ser Gly Thr Gly Ser Glu Ala Leu Met Gly Asn Ala Glu 260 265 270 Gly Lys Ser Met Leu Tyr Leu Glu Ala Arg Cys Ile Tyr Val Thr Arg 275 280 285 Gly Ser Gly Val Gln Gly Leu Gln Asn Gly Ser Val Ser Cys Ile Gly 290 295 300 Met Pro Gly Ser Leu Pro Gly Gly Ile Arg Ala Val Leu Ala Glu Asn 305 310 315 320 Leu Ile Ala Met Leu Leu Asp Leu Glu Cys Ala Ser Ala Asn Asp Gln 325 330 335 Thr Phe Ser His Ser Glu Tyr Arg Arg Thr Ala Arg Thr Leu Met Gln 340 345 350 Met Leu Pro Gly Thr Asp Phe Ile Phe Ser Gly Tyr Ser Ala Val Pro 355 360 365 Asn Cys Asp Asn Met Phe Ala Gly Ser Asn Phe Asp Ala Glu Asp Phe 370 375 380 Asp Asp Tyr Asn Ala Leu Gln Arg Asp Leu Lys Ile Asp Gly Gly Leu 385 390 395 400 Lys Pro Val Thr Glu Asp Glu Ile Val Lys Val Arg Asn Lys Ala Ala 405 410 415 Arg Ala Ile Gln Gly Leu Phe Lys Glu Leu Asp Leu Pro Glu Ile Thr 420 425 430 Asp Glu Glu Val Glu Ala Ala Thr Tyr Ala His Gly Ser Val Asp Met 435 440 445 Pro Ala Arg Asn Val Val Glu Asp Leu Lys Ala Ala Glu Glu Leu Leu 450 455 460 Ser Ser Gly Ile Thr Gly Val Asp Leu Val Lys Gly Leu Ser Arg Ser 465 470 475 480 Gly Phe Asp Asp Val Ala Glu His Val Leu Gly Met Leu Lys Gln Arg 485 490 495 Val Ser Gly Asp Tyr Leu Gln Thr Ser Ala Ile Leu Asp Lys Gly Phe 500 505 510 Lys Ile Lys Ser Ala Ile Asn Asp Arg Asn Asp Tyr Met Gly Pro Gly 515 520 525 Ser Gly Tyr Arg Ile Ser Glu Glu Arg Trp Glu Glu Ile Lys Asn Ile 530 535 540 Pro Ser Ala Ile Lys Pro Glu Ser Ile Glu 545 550 <210> 212 <211> 187 <212> PRT <213> Ilyobacter polytropus DhaB2 protein <400> 212 Met Glu Asn Lys Phe Val Pro Ser Val Lys Ile Glu Glu Ile Gly Glu 1 5 10 15 Ala Lys Lys Gly Ser Arg Ser Glu Glu Val Val Ile Gly Leu Ala Pro 20 25 30 Ala Phe Lys Lys Phe Gln His Lys Thr Ile Thr Asp Val Pro His Asp 35 40 45 Glu Val Leu Thr Glu Leu Ile Ala Gly Ile Glu Glu Glu Gly Leu Lys 50 55 60 Ala Arg Ile Val Arg Val Thr Arg Thr Ser Asp Val Ser Phe Met Ala 65 70 75 80 Leu Asp Ala Ala Lys Leu Ser Gly Ser Gly Ile Gly Ile Gly Ile Gln 85 90 95 Ser Lys Gly Thr Thr Val Ile His Gln Lys Asp Leu Leu Pro Leu Asn 100 105 110 Asn Leu Glu Leu Phe Pro Gln Ala Pro Leu Leu Thr Pro Glu Thr Phe 115 120 125 Arg Leu Ile Gly Lys Asn Ala Ala Lys Tyr Ala Lys Gly Glu Ser Pro 130 135 140 Asn Pro Val Pro Val Ala Ser Asp Gln Met Ala Arg Pro Lys Tyr Gln 145 150 155 160 Ala Lys Ala Ala Leu Leu His Ile Lys Glu Thr Lys His Val Val Gln 165 170 175 His Gly Lys Pro Val Glu Ile Lys Tyr Glu Phe 180 185 <210> 213 <211> 143 <212> PRT <213> Ilyobacter polytropus DhaB3 protein <400> 213 Met Asn Ile Asp Val Lys Asn Ile Asn Pro Ile Ser Asp Tyr Pro Leu 1 5 10 15 Gly Glu Lys Arg Lys Glu Trp Leu Lys Thr Ser Thr Gly Lys Thr Leu 20 25 30 Asp Glu Ile Thr Leu Glu Asn Val Ile Asn Gly Asp Ile Lys Pro Glu 35 40 45 Asp Ile Arg Ile Ser Pro Glu Thr Leu Lys Leu Gln Gly Glu Ile Ala 50 55 60 Lys Lys Gly Asn Arg Pro Thr Ile Thr Lys Asn Phe Glu Arg Ala Ser 65 70 75 80 Glu Met Val Ala Ile Pro Asp Asp Lys Ile Leu Ala Thr Tyr Asn Ala 85 90 95 Leu Arg Pro Tyr Arg Ser Ser Lys Glu Glu Leu Phe Glu Ile Ala Asp 100 105 110 Glu Leu Glu Ser Lys Tyr Ser Ala Val Val Ile Ser Ala Phe Ile Lys 115 120 125 Glu Ala Ala Glu Val Tyr Glu Gln Arg Gly Gln Leu Arg Lys Asp 130 135 140 <210> 214 <211> 1665 <212> DNA <213> Ilyobacter polytropus dhaB1 gene <400> 214 atgaaatcaa aaagatttga agtattgaag gaacgtcctg taaataaaga tggctttata 60 agtgaatgga tagaagaagg actaatcgca atggaaagtc ctaacgatcc taatccaagt 120 ttgaaaatag aaaatggtca aataacagag ttagacggta aaagcagaga agaatttgac 180 atgatcgaca gatttatagc agattatgca ataaatatgg aaaatgctga aaaagctatg 240 aaaatgtcat ctatggaaat atctaaaaaa ctagtagaca taaatgtatc aagagatgaa 300 gtgctggaaa taacaacagg aattacccca gcaaaaataa ttaaagttat ggaacacatg 360 aatgttgtag agatgatgat ggccgtacaa aaaatgagag ccagaaaaac tccttccaat 420 cagtgtcatg taactaactt gagagacaat cctgtattaa ttgccgctga tgctgccgaa 480 gcgtcagtaa gaggttttga tgaacaggag actacaatcg gtatagtaag atatgcacct 540 ttcaatgcca tctcaatatt tgtaggttca caagtaggta gaggaggaat actgactcag 600 tgttctgtag aagaagctac tgaattagag cttggaatga aaggattcac aagttatgca 660 gaaacagtgt ctgtatatgg tacagagcaa gtgtttatag acggtgacga cactccttgg 720 tcaaaagcct tccttgcttc agcatatgca tcaagaggat taaaaatgag atttacatct 780 ggaactggtt cagaggctct tatgggaaat gctgaaggga aatcaatgct ttaccttgaa 840 gcaagatgta tctacgtaac aagagggtct ggagtacaag gactacaaaa tggttctgta 900 agctgcatag ggatgcctgg gtcactacct ggaggaataa gggctgtact ggctgaaaac 960 ctgatagcaa tgttacttga cttagaatgt gcatcagcaa atgaccagac attctctcac 1020 tcagaatata gaaggacagc aagaactcta atgcagatgc ttcctggaac agacttcata 1080 ttctcaggat atagtgccgt accaaactgt gataacatgt ttgctggatc aaattttgat 1140 gcagaggatt ttgatgacta taatgctctt cagagagacc ttaaaataga cggtggttta 1200 aaacctgtaa ctgaagatga gattgtcaaa gtaagaaata aagcagccag agcaatacag 1260 gggttattca aagaacttga tcttcctgaa ataacagatg aagaagtgga agcagcaaca 1320 tatgcccacg gaagtgttga tatgcctgca agaaatgtgg ttgaagattt aaaagcggca 1380 gaagaacttt taagctctgg aataacagga gtagatcttg ttaaaggact tagcagaagc 1440 ggatttgacg atgtagctga gcatgtttta ggtatgttaa aacagagagt ttcaggagat 1500 tacctgcaaa cttcagctat attagacaaa ggctttaaaa taaagagtgc cataaacgat 1560 agaaatgatt acatgggtcc tggaagcgga tatagaataa gcgaggaaag atgggaagag 1620 atcaaaaata tcccatcagc tataaaacca gaaagtatag aatag 1665 <210> 215 <211> 564 <212> DNA <213> Ilyobacter polytropus dhaB2 gene <400> 215 atggaaaata aatttgtacc atctgtaaag atagaagaaa tcggagaagc aaaaaaagga 60 agcagatctg aagaagtagt tataggactg gctcctgcat ttaaaaaatt tcaacataaa 120 acaataacag atgtccctca cgatgaagtc ctgactgaac ttatcgcagg tatagaggaa 180 gagggattaa aggcaagaat cgtaagagta acaagaactt ctgatgtttc atttatggcg 240 ctggatgctg caaagttaag tggttctgga ataggaatag gaattcagtc aaagggaaca 300 acagtaatcc accaaaagga tctgcttcct ctaaacaatc tagaactttt cccacaggct 360 ccactattaa cacctgaaac attcagatta ataggaaaaa atgctgcaaa atatgcaaag 420 ggagaatctc caaatccagt acctgtagcc agtgaccaga tggcgagacc taaatatcag 480 gcaaaagcag cattactaca tataaaagag acaaaacatg tcgttcaaca cggaaaacca 540 gtagagataa agtatgaatt ttag 564 <210> 216 <211> 432 <212> DNA <213> Ilyobacter polytropus dhaB3 gene <400> 216 atgaatatag atgttaaaaa tataaatcca atctctgatt atccattagg agaaaagaga 60 aaagaatggt tgaaaacatc cacaggtaaa actttggatg aaataacttt agaaaatgta 120 ataaatggag atataaagcc tgaagatata agaatctcac ctgaaactct aaaattacag 180 ggagagatag caaagaaagg taacaggcca actataacaa agaactttga aagagccagt 240 gaaatggttg ccattccaga tgataaaata ttagcaactt acaacgcttt gagaccttac 300 agatcttcaa aggaagaatt atttgaaata gccgatgaac tagaaagtaa gtattcagct 360 gttgtaatat ctgcatttat caaggaagcc gcagaagttt atgaacaaag aggtcaactt 420 agaaaagatt ag 432 <210> 217 <211> 607 <212> PRT <213> K.pneumoniae gdrA protein <400> 217 Met Pro Leu Ile Ala Gly Ile Asp Ile Gly Asn Ala Thr Thr Glu Val 1 5 10 15 Ala Leu Ala Ser Asp Asp Pro Gln Ala Arg Ala Phe Val Ala Ser Gly 20 25 30 Ile Val Ala Thr Thr Gly Met Lys Gly Thr Arg Asp Asn Ile Ala Gly 35 40 45 Thr Leu Ala Ala Leu Glu Gln Ala Leu Ala Lys Thr Pro Trp Ser Val 50 55 60 Ser Asp Val Ser Arg Ile Tyr Leu Asn Glu Ala Ala Pro Val Ile Gly 65 70 75 80 Asp Val Ala Met Glu Thr Ile Thr Glu Thr Ile Ile Thr Glu Ser Thr 85 90 95 Met Ile Gly His Asn Pro Gln Thr Pro Gly Gly Val Gly Val Gly Val 100 105 110 Gly Thr Thr Ile Ala Leu Gly Arg Leu Ala Thr Leu Pro Ala Ala Gln 115 120 125 Tyr Ala Glu Gly Trp Ile Val Leu Ile Asp Asp Ala Val Asp Phe Leu 130 135 140 Asp Ala Val Trp Trp Leu Asn Glu Ala Leu Asp Arg Gly Ile Asn Val 145 150 155 160 Val Ala Ala Ile Leu Lys Lys Asp Asp Gly Val Leu Val Asn Asn Arg 165 170 175 Leu Arg Lys Thr Leu Pro Val Val Asp Glu Val Thr Leu Leu Glu Gln 180 185 190 Val Pro Glu Gly Val Met Ala Ala Val Glu Val Ala Ala Pro Gly Gln 195 200 205 Val Val Arg Ile Leu Ser Asn Pro Tyr Gly Ile Ala Thr Phe Phe Gly 210 215 220 Leu Ser Pro Glu Glu Thr Gln Ala Ile Val Pro Ile Ala Arg Ala Leu 225 230 235 240 Ile Gly Asn Arg Ser Ala Val Val Leu Lys Thr Pro Gln Gly Asp Val 245 250 255 Gln Ser Arg Val Ile Pro Ala Gly Asn Leu Tyr Ile Ser Gly Glu Lys 260 265 270 Arg Arg Gly Glu Ala Asp Val Ala Glu Gly Ala Glu Ala Ile Met Gln 275 280 285 Ala Met Ser Ala Cys Ala Pro Val Arg Asp Ile Arg Gly Glu Pro Gly 290 295 300 Thr His Ala Gly Gly Met Leu Glu Arg Val Arg Lys Val Met Ala Ser 305 310 315 320 Leu Thr Asp His Glu Met Ser Ala Ile Tyr Ile Gln Asp Leu Leu Ala 325 330 335 Val Asp Thr Phe Ile Pro Arg Lys Val Gln Gly Gly Met Ala Gly Glu 340 345 350 Cys Ala Met Glu Asn Ala Val Gly Met Ala Ala Met Val Lys Ala Asp 355 360 365 Arg Leu Gln Met Gln Val Ile Ala Arg Glu Leu Ser Ala Arg Leu Gln 370 375 380 Thr Glu Val Val Val Gly Gly Val Glu Ala Asn Met Ala Ile Ala Gly 385 390 395 400 Ala Leu Thr Thr Pro Gly Cys Ala Ala Pro Leu Ala Ile Leu Asp Leu 405 410 415 Gly Ala Gly Ser Thr Asp Ala Ala Ile Val Asn Ala Glu Gly Gln Ile 420 425 430 Thr Ala Val His Leu Ala Gly Ala Gly Asn Met Val Ser Leu Leu Ile 435 440 445 Lys Thr Glu Leu Gly Leu Glu Asp Leu Ser Leu Ala Glu Ala Ile Lys 450 455 460 Lys Tyr Pro Leu Ala Lys Val Glu Ser Leu Phe Ser Ile Arg His Glu 465 470 475 480 Asn Gly Ala Val Glu Phe Phe Arg Glu Ala Leu Ser Pro Ala Val Phe 485 490 495 Ala Lys Val Val Tyr Ile Lys Glu Gly Glu Leu Val Pro Ile Asp Asn 500 505 510 Ala Ser Pro Leu Glu Lys Ile Arg Leu Val Arg Arg Gln Ala Lys Glu 515 520 525 Lys Val Phe Val Thr Asn Cys Leu Arg Ala Leu Arg Gln Val Ser Pro 530 535 540 Gly Gly Ser Ile Arg Asp Ile Ala Phe Val Val Leu Val Gly Gly Ser 545 550 555 560 Ser Leu Asp Phe Glu Ile Pro Gln Leu Ile Thr Glu Ala Leu Ser His 565 570 575 Tyr Gly Val Val Ala Gly Gln Gly Asn Ile Arg Gly Thr Glu Gly Pro 580 585 590 Arg Asn Ala Val Ala Thr Gly Leu Leu Leu Ala Gly Gln Ala Asn 595 600 605 <210> 218 <211> 117 <212> PRT <213> K.pneumoniae gdrB protein <400> 218 Met Ser Leu Ser Pro Pro Gly Val Arg Leu Phe Tyr Asp Pro Arg Gly 1 5 10 15 His His Ala Gly Ala Ile Asn Glu Leu Cys Trp Gly Leu Glu Glu Gln 20 25 30 Gly Val Pro Cys Gln Thr Ile Thr Tyr Asp Gly Gly Gly Asp Ala Ala 35 40 45 Ala Leu Gly Ala Leu Ala Ala Arg Ser Ser Pro Leu Arg Val Gly Ile 50 55 60 Gly Leu Ser Ala Ser Gly Glu Ile Ala Leu Thr His Ala Gln Leu Pro 65 70 75 80 Ala Asp Ala Pro Leu Ala Thr Gly His Val Thr Asp Ser Asp Asp His 85 90 95 Leu Arg Thr Leu Gly Ala Asn Ala Gly Gln Leu Val Lys Val Leu Pro 100 105 110 Leu Ser Glu Arg Asn 115 <210> 219 <211> 607 <212> PRT <213> Ilyobacter polytropus gdrA protien <400> 219 Met Lys Ile Ile Val Gly Val Asp Ile Gly Asn Ala Thr Thr Glu Val 1 5 10 15 Ala Leu Ala Lys Val Asp Asn Ile Glu Cys Lys Phe Leu Ser Ser Ala 20 25 30 Leu His Glu Thr Thr Gly Leu Lys Gly Thr Lys Asp Asn Val Leu Gly 35 40 45 Ile Lys Arg Ala Ile Lys Lys Ala Met Lys Arg Ala Asp Leu Lys Asn 50 55 60 Ala Asp Leu Ser Leu Ile Arg Ile Asn Glu Ala Thr Pro Val Ile Gly 65 70 75 80 Asp Val Ser Met Glu Thr Ile Thr Glu Thr Ile Ile Thr Glu Ser Thr 85 90 95 Met Ile Gly His Asn Pro Ser Thr Pro Gly Gly Ile Gly Leu Gly Ile 100 105 110 Gly Glu Thr Ile Leu Phe Gln Glu Leu Gly Asn Phe Glu Asn Asp Lys 115 120 125 Asp Tyr Ile Val Ile Val Glu Lys Ser Phe Ser Phe Leu Glu Val Ala 130 135 140 His Arg Ile Asn Glu Ala Phe Lys Asn Gly Cys Lys Ile Lys Gly Ala 145 150 155 160 Ile Ile Gln Lys Asp Asp Gly Val Leu Ile Asn Asn Arg Leu Ile Asn 165 170 175 Lys Ile Pro Ile Val Asp Glu Val Leu Phe Val Lys Lys Val Pro Thr 180 185 190 Gly Met Lys Ala Ala Val Glu Val Ala Pro Gln Gly Lys Ile Ile Glu 195 200 205 Val Ile Ser Asn Pro Tyr Gly Ile Ala Thr Ile Phe Ser Leu Thr Ser 210 215 220 Glu Glu Thr Lys Lys Ile Val Pro Ile Ser Lys Ala Leu Ile Gly Asn 225 230 235 240 Arg Ser Gly Val Val Ile Lys Thr Pro His Gly Asp Val Lys Glu Lys 245 250 255 Val Ile Pro Ala Gly Arg Ile Gln Ile Asp Gly Asn Tyr Arg Ser Lys 260 265 270 Ser Val Asn Ile Glu Glu Gly Ser Lys Arg Ile Met Lys Ala Leu Gly 275 280 285 Ser Ile Glu His Val Gln Asp Ile Asn Gly Glu Ser Gly Thr Asn Ile 290 295 300 Gly Gly Met Leu Lys Asn Val Lys Ser Val Met Gly Asn Phe Thr Asn 305 310 315 320 Glu Ser Ile Asp Asn Ile Lys Ile Lys Asp Ile Leu Ala Val Asp Thr 325 330 335 Phe Val Pro Gln Lys Ile Lys Gly Gly Ile Ala Glu Glu Phe Val Phe 340 345 350 Glu Asn Ala Val Gly Ile Ala Ala Met Val Asn Thr Lys Lys Asn Gln 355 360 365 Met Ser Glu Val Ala Lys Glu Ile Glu Lys Glu Leu Gly Val Lys Val 370 375 380 Glu Val Gly Gly Val Glu Ala Asp Met Ala Ile Thr Gly Ala Leu Thr 385 390 395 400 Thr Pro Gly Thr Gly Thr Pro Leu Val Ile Val Asp Ile Gly Ala Gly 405 410 415 Ser Thr Asp Ala Cys Ser Ile Asp Arg Tyr Gly Asn Lys Glu Leu Val 420 425 430 His Leu Ala Gly Ala Gly Asn Met Thr Thr Leu Leu Ile Gln Lys Glu 435 440 445 Leu Gly Ile Glu Asp Phe Asn Leu Ala Glu Asp Ile Lys Lys Tyr Pro 450 455 460 Leu Ala Lys Val Glu Ser Leu Phe Tyr Ile Arg His Glu Asp Gly Asn 465 470 475 480 Val Gln Phe Phe Glu Asn Ser Leu Ser Pro Lys Val Phe Ala Lys Asn 485 490 495 Val Leu Ile Lys Glu Gly Glu Leu Ile Pro Ile Asp Leu Asp Met Ser 500 505 510 Leu Glu Lys Ile Arg Ile Ile Arg Arg Ser Ala Lys Arg Lys Ile Phe 515 520 525 Ile Thr Asn Val Leu Arg Ser Leu Arg Lys Val Ser His Thr Lys Asn 530 535 540 Ile Arg Asp Phe Glu Phe Val Val Ile Val Gly Gly Ser Ala Leu Asp 545 550 555 560 Phe Glu Ile Ser Gln Met Ile Thr Glu Ala Leu Ser Glu Tyr Gly Ile 565 570 575 Val Ala Gly Cys Gly Asn Ile Arg Gly Thr Glu Gly Pro Arg Asn Ala 580 585 590 Val Ala Thr Gly Leu Val Met Gly Val Asn Asp Gly Gln Gln Ala 595 600 605 <210> 220 <211> 107 <212> PRT <213> Ilyobacter polytropus gdrB <400> 220 Met Asp Asn Arg Pro Asn Ile Thr Leu Phe Cys Ser Asp Asn Ile Asp 1 5 10 15 Arg Glu Tyr Ile Asn Glu Ile Leu Trp Gly Ile Glu Glu Glu Glu Ile 20 25 30 Pro Tyr Leu Leu Lys Ile Val Pro Ser Lys Glu Val Val Lys Glu Asn 35 40 45 Tyr Val Ser Gly Thr Leu Glu Ile Gly Ile Gly Val Leu Glu Asn Gly 50 55 60 Asp Ala Leu Leu Thr Thr Arg Lys Tyr Asp Lys Glu Tyr Ile Gln Lys 65 70 75 80 Ala Asn Ile Phe Val Glu Lys Asn Lys Leu Arg Asp Leu Gly Ser Asn 85 90 95 Gly Ala Arg Leu Val Lys Gly Leu Pro Leu Arg 100 105 <210> 221 <211> 1824 <212> DNA <213> Ilyobacter polytropus gdrA gene <400> 221 atgaagatca tagtgggtgt agatattgga aatgctacaa cagaagtagc tttggcaaag 60 gtagacaata tagaatgtaa gtttttatcc agtgccttac atgaaacaac aggtttaaaa 120 ggtactaaag ataatgtttt gggaataaaa agagccatta agaaggcaat gaaaagagct 180 gatttaaaaa atgcagattt atctttaatc aggataaatg aagctactcc tgttatagga 240 gacgtttcta tggaaactat aacagaaaca ataattacag agtctactat gattggacat 300 aacccttcaa ctcctggggg aataggtctt gggataggag aaacaatcct attccaagag 360 cttggaaatt ttgaaaatga taaagattac atagtaatag tggaaaaaag tttcagcttc 420 ttagaggtag ctcacagaat caatgaagct tttaaaaatg gatgcaaaat aaagggtgct 480 attattcaaa aagatgatgg ggttctcata aataacagac tcataaataa aatccccata 540 gttgatgagg tactttttgt taaaaaagta cctacaggga tgaaggctgc tgtagaagta 600 gctccacagg gaaaaataat agaggttatt tcaaatccat atggcattgc cacaattttt 660 tccctcactt cagaagagac taaaaaaata gttcctattt ctaaagcact tataggcaac 720 aggtctggag tagttatcaa gacacctcac ggagatgtaa aagagaaggt tatccctgct 780 ggaaggatac agattgacgg aaactacagg tcaaaaagtg taaatataga agagggttcc 840 aaaagaataa tgaaagccct gggaagtatt gagcatgtcc aagatataaa tggagaatct 900 ggaaccaata tcggaggaat gctaaaaaat gtaaaaagtg taatggggaa tttcaccaat 960 gagtccattg ataatataaa aataaaagac atattggcag tagatacctt tgtcccacaa 1020 aagataaagg ggggaattgc agaagaattt gtatttgaaa atgctgtagg aatagctgca 1080 atggtaaata ccaaaaaaaa tcaaatgtcc gaagtagcga aagagattga aaaagaactg 1140 ggagtaaaag tagaagtagg aggagtagag gcagatatgg ctataaccgg tgctctaact 1200 actccaggca caggaacacc tctggtaatt gtagatatag gagcaggttc gacagatgca 1260 tgttccattg acagatatgg aaataaagaa ctggttcatc tggccggagc tggtaatatg 1320 acaacacttc ttattcaaaa agagctgggt atagaggatt ttaatcttgc tgaagatata 1380 aaaaaatatc ctctggcaaa agtagaatct ctattttata taagacacga ggatggaaat 1440 gttcaatttt ttgaaaactc tctttctccg aaagtatttg ctaaaaatgt ccttataaaa 1500 gaaggtgaac ttattccaat cgaccttgat atgtctctgg aaaaaatcag aattatcaga 1560 aggtctgcca aaagaaaaat ttttataacc aatgtactta gatcattaag gaaagtttct 1620 catacaaaaa atattaggga ttttgaattt gtagttattg ttggaggatc tgcattggat 1680 tttgaaatat ctcagatgat aactgaagct ttatctgagt atggaatagt agcaggatgc 1740 ggaaatataa gaggaacaga gggccctaga aatgctgtag ccactggact tgtaatgggg 1800 gtgaatgatg gacaacaggc ctaa 1824 <210> 222 <211> 324 <212> DNA <213> Ilyobacter polytropus gdrB gene <400> 222 atggacaaca ggcctaatat aacattattt tgctcagata atattgacag ggaatatatt 60 aatgaaattt tgtggggtat agaggaggaa gagataccat atcttctgaa aattgtacct 120 tctaaagaag ttgtcaaaga aaattatgtt tcaggaactc tagagatagg tatcggagta 180 ttagaaaatg gcgacgccct tctaacaaca aggaagtacg ataaggaata tatacaaaag 240 gcaaacattt ttgtagaaaa aaataaattg agagatttag gaagcaacgg agcaagactt 300 gtaaagggtc tgccacttag ataa 324 <210> 223 <211> 1824 <212> DNA <213> K.pneumoniae gdrA gene <400> 223 atgccgttaa tagccgggat tgatatcggc aacgccacca ccgaggtggc gctggcgtcc 60 gacgacccgc aggcgagggc gtttgttgcc agcgggatcg tcgcgacgac gggcatgaaa 120 gggacgcggg acaatatcgc cgggaccctc gccgcgctgg agcaggccct ggcgaaaaca 180 ccgtggtcgg tgagcgatgt ctctcgcatc tatcttaacg aagccgcgcc ggtgattggc 240 gatgtggcga tggagaccat caccgagacc attatcaccg aatcgaccat gatcggtcat 300 aacccgcaga cgccgggcgg ggtgggcgtt ggcgtgggga cgactatcgc cctcgggcgg 360 ctggcgacgc tgccggcggc gcagtatgcc gaggggtgga tcgtactgat tgacgacgcc 420 gtcgatttcc ttgacgccgt gtggtggctc aatgaggcgc tcgaccgggg gatcaacgtg 480 gtggcggcga tcctcaaaaa ggacgacggc gtgctggtga acaaccgcct gcgtaaaacc 540 ctgccggtgg tagatgaagt gacgctgctg gagcaggtcc ccgagggggt aatggcggcg 600 gtggaagtgg ccgcgccggg ccaggtggtg cggatcctgt cgaatcccta cgggatcgcc 660 accttcttcg ggctaagccc ggaagagacc caggccatcg tccccatcgc ccgcgccctg 720 attggcaacc gttcagcggt ggtgctcaag accccgcagg gggatgtgca gtcgcgggtg 780 atcccggcgg gcaacctcta cattagcggc gaaaagcgcc gcggagaggc cgatgtcgcc 840 gagggcgcgg aagccatcat gcaggcgatg agcgcctgcg ctccggtacg cgacatccgc 900 ggcgaaccgg gcactcacgc cggcggcatg cttgagcggg tgcgcaaggt aatggcgtcc 960 ctgaccgacc atgagatgag cgcgatatac atccaggatc tgctggcggt ggatacgttt 1020 attccgcgca aggtgcaggg cgggatggcc ggcgagtgcg ccatggaaaa tgccgtcggg 1080 atggcggcga tggtgaaagc ggatcgtctg caaatgcagg ttatcgcccg cgaactgagc 1140 gcccgactgc agaccgaggt ggtggtgggc ggcgtggagg ccaacatggc catcgccggg 1200 gcgttaacca ctcccggctg tgcggcgccg ctggcgatcc tcgacctcgg cgccggctcg 1260 acggatgcgg cgatcgtcaa cgcggagggg cagataacgg cggtccatct cgccggggcg 1320 gggaatatgg tcagcctgtt gattaaaacc gagctgggcc tcgaggatct ttcgctggcg 1380 gaagcgataa aaaaataccc gctggccaaa gtggaaagcc tgttcagtat tcgtcacgag 1440 aatggcgcgg tggagttctt tcgggaagcc ctcagcccgg cggtgttcgc caaagtggtg 1500 tacatcaagg agggcgaact ggtgccgatc gataacgcca gcccgctgga aaaaattcgt 1560 ctcgtgcgcc ggcaggcgaa agagaaagtg tttgtcacca actgcctgcg cgcgctgcgc 1620 caggtctcac ccggcggttc cattcgcgat atcgcctttg tggtgctggt gggcggctca 1680 tcgctggact ttgagatccc gcagcttatc acggaagcct tgtcgcacta tggcgtggtc 1740 gccgggcagg gcaatattcg gggaacagaa gggccgcgca acgcggtcgc caccgggctg 1800 ctactggccg gtcaggcgaa ttaa 1824 <210> 224 <211> 354 <212> DNA <213> K.pneumoniae gdrB gene <400> 224 atgtcgcttt caccgccagg cgtacgcctg ttttacgatc cgcgcgggca ccatgccggc 60 gccatcaatg agctgtgctg ggggctggag gagcaggggg tcccctgcca gaccataacc 120 tatgacggag gcggtgacgc cgctgcgctg ggcgccctgg cggccagaag ctcgcccctg 180 cgggtgggta ttgggctcag cgcgtccggc gagatagccc tcactcatgc ccagctgccg 240 gcggacgcgc cgctggctac cggacacgtc accgatagcg acgatcatct gcgtacgctc 300 ggcgccaacg ccgggcagct ggttaaagtc ctgccgttaa gtgagagaaa ctga 354 <210> 225 <211> 314 <212> PRT <213> Corynebacterium glutamicum LDH <400> 225 Met Lys Glu Thr Val Gly Asn Lys Ile Val Leu Ile Gly Ala Gly Asp 1 5 10 15 Val Gly Val Ala Tyr Ala Tyr Ala Leu Ile Asn Gln Gly Met Ala Asp 20 25 30 His Leu Ala Ile Ile Asp Ile Asp Glu Lys Lys Leu Glu Gly Asn Val 35 40 45 Met Asp Leu Asn His Gly Val Val Trp Ala Asp Ser Arg Thr Arg Val 50 55 60 Thr Lys Gly Thr Tyr Ala Asp Cys Glu Asp Ala Ala Met Val Val Ile 65 70 75 80 Cys Ala Gly Ala Ala Gln Lys Pro Gly Glu Thr Arg Leu Gln Leu Val 85 90 95 Asp Lys Asn Val Lys Ile Met Lys Ser Ile Val Gly Asp Val Met Asp 100 105 110 Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile 115 120 125 Leu Thr Tyr Ala Val Trp Lys Phe Ser Gly Leu Glu Trp Asn Arg Val 130 135 140 Ile Gly Ser Gly Thr Val Leu Asp Ser Ala Arg Phe Arg Tyr Met Leu 145 150 155 160 Gly Glu Leu Tyr Glu Val Ala Pro Ser Ser Val His Ala Tyr Ile Ile 165 170 175 Gly Glu His Gly Asp Thr Glu Leu Pro Val Leu Ser Ser Ala Thr Ile 180 185 190 Ala Gly Val Ser Leu Ser Arg Met Leu Asp Lys Asp Pro Glu Leu Glu 195 200 205 Gly Arg Leu Glu Lys Ile Phe Glu Asp Thr Arg Asp Ala Ala Tyr His 210 215 220 Ile Ile Asp Ala Lys Gly Ser Thr Ser Tyr Gly Ile Gly Met Gly Leu 225 230 235 240 Ala Arg Ile Thr Arg Ala Ile Leu Gln Asn Gln Asp Val Ala Val Pro 245 250 255 Val Ser Ala Leu Leu His Gly Glu Tyr Gly Glu Glu Asp Ile Tyr Ile 260 265 270 Gly Thr Pro Ala Val Val Asn Arg Arg Gly Ile Arg Arg Val Val Glu 275 280 285 Leu Glu Ile Thr Asp His Glu Met Glu Arg Phe Lys His Ser Ala Asn 290 295 300 Thr Leu Arg Glu Ile Gln Lys Gln Phe Phe 305 310 <210> 226 <211> 3267 <212> DNA <213> Artificial Sequence <220> <223> pKD4 vector <400> 226 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta 60 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctca cgctgccgca 120 agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga 180 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa 240 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg 300 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga 360 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat 420 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 480 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 540 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 600 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 660 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 720 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 780 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 840 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 900 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 960 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 1020 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 1080 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 1140 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 1200 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 1260 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 1320 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 1380 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccagct tcaaaagcgc 1440 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat 1500 tcatatggac catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat 1560 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac 1620 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa 1680 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct 1740 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg 1800 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga 1860 aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc ggccgcaaaa 1920 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1980 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 2040 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 2100 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 2160 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 2220 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 2280 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 2340 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 2400 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 2460 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 2520 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 2580 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 2640 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 2700 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 2760 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 2820 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 2880 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 2940 cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg tagtgtgggg 3000 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa 3060 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa 3120 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg 3180 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt 3240 tgcgtggcca gtgccaagct tgcatgc 3267 <210> 227 <211> 58 <212> DNA <213> Artificial Sequence <220> <223> ackAKF primer <400> 227 cgtagtgatc gatgagtctg ttattcaggg tatcaaaggt gtaggctgga gctgcttc 58 <210> 228 <211> 56 <212> DNA <213> Artificial Sequence <220> <223> ackAKR primer <400> 228 caatccctgc acccagttct acaccctgag acgctgattc cggggatccg tcgacc 56 <210> 229 <211> 6329 <212> DNA <213> Artificial Sequence <220> <223> pKD46 vector <400> 229 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 60 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 120 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 180 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 240 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 300 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 360 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 420 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 480 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 540 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 600 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 660 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 720 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 780 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 840 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 900 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 960 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1020 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1080 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1140 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1200 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga 1260 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc 1320 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat 1380 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga 1440 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc 1500 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt 1560 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac 1620 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc 1680 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa 1740 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc 1800 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc 1860 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc 1920 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca 1980 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa 2040 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg 2100 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga 2160 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact 2220 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt 2280 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc 2340 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa 2400 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca 2460 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc 2520 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc 2580 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg 2640 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg 2700 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga 2760 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg 2820 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg 2880 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga 2940 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc 3000 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg 3060 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat 3120 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt 3180 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca 3240 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt 3300 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg 3360 ccaataccag tagaaacaga cgaagaatcc atgggtatgg acagttttcc ctttgatatg 3420 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca 3480 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg 3540 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact 3600 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt 3660 ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc 3720 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc 3780 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt 3840 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa 3900 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat 3960 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat 4020 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa 4080 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa 4140 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc 4200 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct 4260 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct 4320 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc 4380 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc 4440 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag 4500 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac 4560 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg 4620 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa 4680 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta 4740 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa 4800 aggcttaagt agcaccctcg caagctcggt tgcggccgca atcgggcaaa tcgctgaata 4860 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc 4920 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg 4980 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt 5040 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc 5100 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa 5160 tgcacccagt aaggcagcgg tatcatcaac ggggtctgac gctcagtgga acgaaaactc 5220 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5280 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5340 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5400 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5460 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 5520 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 5580 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 5640 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 5700 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 5760 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 5820 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 5880 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 5940 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6000 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6060 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6120 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6180 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6240 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6300 gcgcacattt ccccgaaaag tgccacctg 6329 <210> 230 <211> 9332 <212> DNA <213> Artificial Sequence <220> <223> pCP20 vector <400> 230 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca 60 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact 120 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg 180 gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgcc 240 acaggtgcgg ttgctggcgc taaccgtttt tatcaggctc tgggaggcag aataaatgat 300 catatcgtca attattacct ccacggggag agcctgagca aactggcctc aggcatttga 360 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata 420 agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt 480 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac 540 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat 600 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc 660 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg 720 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg 780 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg 840 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca 900 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca 960 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc 1020 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg 1080 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact 1140 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat 1200 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa 1260 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga 1320 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca 1380 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg 1440 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt 1500 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg 1560 caaaagcacc gccggacatc agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 1620 ccgggggact gttgggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag 1680 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa 1740 aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt 1800 aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa 1860 ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt 1920 ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc 1980 tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga 2040 atttacagat acccacaact caaaggaaaa ggactagtaa ttatcattga ctagcccatc 2100 tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg 2160 ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc 2220 taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac 2280 ggacggtatc gttcacttat aaccaatacg ttcagatgat gaacatcagt agggaaaatg 2340 cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga 2400 atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg 2460 aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat 2520 tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt 2580 atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta 2640 gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc 2700 ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat 2760 tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata 2820 gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa 2880 taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa acactacacg 2940 atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc 3000 aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac 3060 tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc 3120 agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta catatcaaag 3180 ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt 3240 ttacgagttt ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaacagat 3300 gaacagcatg taacacctaa tagaacaggt gaaaccagta aaacaaagca actagaacat 3360 gaaattgaac acctgagaca acttgttaca gctcaacagt cacacataga cagcctgaaa 3420 caggcgatgc tgcttatcga atcaaagctg ccgacaacac gggagccagt gacgcctccc 3480 gtggggaaaa aatcatggca attctggaag aaatagcgcc tgtttcgttt caggcaggtt 3540 atcagggagt gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca gcccgtaatg 3600 gtgatttacc agcgtctgcc aggcatcaat tctaggcctg tctgcgcggt cgtagtacgg 3660 ctggaggcgt tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg 3720 tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga gaccgggcat 3780 cagtacacgc tcaaagctcg ccatcacttt ttcacgtcct cccggcggca gctccttctc 3840 cgcgaacgac agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc 3900 ccggacttct tccggattac cctgaagcac cgttgcgcct tcgcggttac gctccctccc 3960 cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa atttaactat 4020 catcccgcgc cccctgttcc ctgacagcca gacgcagccg gcgcagctca tccccgatgg 4080 ccatcagtgc ggccaccacc tgaacccggt caccggaaga ccactgcccg ctgttcacct 4140 tacgggctgt ctgattcagg ttatttccga tggcggccag ctgacgcagt aacggcggtg 4200 ccagtgtcgg cagttttccg gaacgggcaa ccggctcccc caggcagacc cgccgcatcc 4260 ataccgccag ttgtttaccc tcacagcgtt caagtaaccg ggcatgttca tcatcagtaa 4320 cccgtattgt gagcatcctc tcgcgtttca tcggtatcat taccccatga acagaaatcc 4380 cccttacacg gaggcatcag tgactaaacg gggtctgacg ctcagtggaa cgaaaactca 4440 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 4500 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 4560 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 4620 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 4680 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 4740 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 4800 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 4860 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 4920 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 4980 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 5040 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 5100 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 5160 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 5220 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5280 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5340 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 5400 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 5460 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 5520 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 5580 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg 5640 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg 5700 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg 5760 cttcctgtcg gccctcattc gtgcgctcta ggatcctcta cgccggacgc atcgtggccg 5820 gcatcaccgg cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg 5880 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta 5940 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg 6000 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg 6060 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag 6120 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca 6180 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg 6240 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt 6300 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa 6360 tccggtgaga atggcagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac 6420 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc 6480 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta 6540 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc 6600 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag 6660 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc 6720 ctttgatatt ggatcatatg catagtaccg agaaactagt gcgaagtagt gatcaggtat 6780 tgctgttatc tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca 6840 atttcccaca acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa 6900 atgtcttcca atgtgagatt ttgggccatt ttttatagca aagattgaat aaggcgcatt 6960 tttcttcaaa gctttattgt acgatctgac taagttatct tttaataatt ggtattcctg 7020 tttattgctt gaagaattgc cggtcctatt tactcgtttt aggactggtt cagaattcct 7080 caaaaattca tccaaatata caagtggatc gatcctaccc cttgcgctaa agaagtatat 7140 gtgcctacta acgcttgtct ttgtctctgt cactaaacac tggattatta ctcccagata 7200 cttattttgg actaatttaa atgatttcgg atcaacgttc ttaatatcgc tgaatcttcc 7260 acaattgatg aaagtagcta ggaagaggaa ttggtataaa gtttttgttt ttgtaaatct 7320 cgaagtatac tcaaacgaat ttagtatttt ctcagtgatc tcccagatgc tttcaccctc 7380 acttagaagt gctttaagca tttttttact gtggctattt cccttatctg cttcttccga 7440 tgattcgaac tgtaattgca aactacttac aatatcagtg atatcagatt gatgtttttg 7500 tccatagtaa ggaataattg taaattccca agcaggaatc aatttcttta atgaggcttc 7560 cagaattgtt gctttttgcg tcttgtattt aaactggagt gatttattga caatatcgaa 7620 actcagcgaa ttgcttatga tagtattata gctcatgaat gtggctctct tgattgctgt 7680 tccgttatgt gtaatcatcc aacataaata ggttagttca gcagcacata atgctatttt 7740 ctcacctgaa ggtctttcaa acctttccac aaactgacga acaagcacct taggtggtgt 7800 tttacataat atatcaaatt gtggcataca acctccttag tacatgcaac cattatcacc 7860 gccagaggta aaatagtcaa cacgcacggt gttagatatt tatcccttgc ggtgatagat 7920 ttaacgtatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg 7980 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc 8040 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt 8100 aaatgcttat aacgccgcat tgcttacaaa aattctcaaa gttagcgttg aagaatttag 8160 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact 8220 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctaa 8280 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag 8340 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa 8400 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg 8460 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga 8520 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga 8580 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggctg 8640 atcggcaagg tgttctggtc ggcgcatagc tgataacaat tgagcaagaa tctgcatttc 8700 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 8760 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 8820 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 8880 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 8940 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 9000 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 9060 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 9120 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 9180 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat 9240 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 9300 tttatcttgt gcaatgtaac atcagagatt tt 9332 <210> 231 <211> 65 <212> DNA <213> Artificial Sequence <220> <223> yqhDKF primer <400> 231 cgccatcatg gcggtgcggc gctgccttcc agttcggtta acacggtgta ggctggagct 60 gcttc 65 <210> 232 <211> 65 <212> DNA <213> Artificial Sequence <220> <223> yqhDKR primer <400> 232 gcgcgagttc tcaataatgg cgcgtttggt gcgaacttcg tggtaattcc ggggatccgt 60 cgacc 65 <210> 233 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> dhaB123_F primer <400> 233 tcatgaaatc aaaaagattt gaagtattga ag 32 <210> 234 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> dhaB123_R primer <400> 234 ggatccctaa tcttttctaa gttgacctct ttgttc 36 <210> 235 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> gdrAB_F primer <400> 235 ggatccaaag gttcggggat agttatgaag 30 <210> 236 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> gdrAB_R primer <400> 236 gagctcttat ctaagtggca gaccctttac aag 33 <210> 237 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> iBAB_Up primer <400> 237 atgtatatct ccttcttata cttaactaat 30 <210> 238 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> iBAB_Dn primer <400> 238 atcggccggc cacgcgatcg ctgacgtcgg taccc 35 <210> 239 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> pduP_F primer <400> 239 gaaggagata tacatatgca gattaatgat attga 35 <210> 240 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> pduP_R primer <400> 240 gcgtggccgg ccgatttaat accagttacg tactg 35 <210> 241 <211> 49 <212> DNA <213> Artificial Sequence <220> <223> primer for MELS_1449 gene <400> 241 tcatcaccac agccaggatc cgatggcact aagagatggg aattcctac 49 <210> 242 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> primer for MELS_1449 gene <400> 242 gcattatgcg gccgcaagct tttatttttc ttcctttatg ccg 43 <210> 243 <211> 43 <212> DNA <213> Artificial Sequence <220> <223> yciA_F primer <400> 243 tcatcaccac agccaagatc tgatgtctac aacacataac gtc 43 <210> 244 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> yciA_R primer <400> 244 gcattatgcg gccgcctcga gttactcaac aggtaaggcg 40

Claims (20)

  1. 유전적으로 조작되지 않은 세포에 비하여 3-히드록시프로피온알데히드 (3-HPA)를 3-히드록시프로피오닐-CoA (3-HP-CoA)로 전환하는 것을 촉매하는 CoA-아실화 알데히드 데히드로게나제(CoA acylating aldehyde dehydrogenase: ALDH), 및 3-HP-CoA를 아크릴릴-CoA (acrylyl-CoA)로 전환하는 것을 촉매하는 3-HP-CoA 데히드라타제의 활성이 증가되어 있는, 아크릴레이트 생산능을 갖는 미생물.
  2. 청구항 1에 있어서, 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소의 활성이 더 증가되어 있는 것인 미생물.
  3. 청구항 1에 있어서, 상기 ALDH는 서열번호 1 내지 20의 아미노산 서열로 이루어진 군으로부터 선택된 하나 이상을 갖는 것인 미생물.
  4. 청구항 1에 있어서, 상기 ALDH는 EC 1.2.1.10, 또는 EC 1.2.1.87에 속하는 것인 미생물.
  5. 청구항 1에 있어서, 상기 ALDH는 프로피온알데히드 데히드로게나제 (propioaldehyde dehydrogenase: pduP)인 것인 미생물.
  6. 청구항 1에 있어서, 상기 3-HP-CoA 데히드라타제는 서열번호 41 내지 119의 아미노산 서열로 이루어진 군으로부터 선택된 하나 이상을 갖는 것인 미생물.
  7. 청구항 1에 있어서, 상기 3-HP-CoA 데히드라타제는 EC 4.2.1.17, EC 4.2.1.55, 및 EC 4.2.1.166를 포함한, EC 4.2.1.-에 속하는 것인 미생물.
  8. 청구항 2에 있어서, 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 서열번호 199 내지 204의 아미노산 서열로 이루어진 군으로부터 선택된 하나 이상을 갖는 것인 미생물.
  9. 청구항 2에 있어서, 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 EC 3.1.2.4를 포함한, EC 3.1.2-에 속하는 것인 미생물.
  10. 청구항 2에 있어서, 상기 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소는 3-HP-CoA 히드롤라제 (3-HP-CoA hydrolase), 또는 3-히드록시이소부티릴-CoA 히드롤라제(3-hydroxyisobutyryl-CoA hydrolase)인 것인 미생물.
  11. 청구항 1에 있어서, ALDH, 및 3-HP-CoA 데히드라타제의 활성의 증가는 이들 효소를 코딩하는 폴리뉴클레오티드의 발현 증가에 의한 것인 미생물.
  12. 청구항 1에 있어서, 상기 미생물은 ALDH, 및 3-HP-CoA 데히드라타제, 또는 아크릴릴-CoA를 아크릴레이트로 전환하는 것을 촉매하는 효소를 코딩하는 폴리뉴클레오티드가 도입된 것인 미생물.
  13. 청구항 1에 있어서, 상기 미생물은 에세리키아 (Esherichia), 루멘박테리아, 코리네박테리움 (Corynebacterium) 속 및 브레비박테리움 (Brevibacterium) 속으로구성된 군으로부터 선택되는 것인 미생물.
  14. 청구항 1에 있어서, 상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소의 활성이 감소된 것인 미생물.
  15. 청구항 1에 있어서, 상기 미생물은 아크릴레이트를 분해하거나 다른 산물로 전환하는 경로에 관여하는 하나 이상의 효소를 코딩하는 유전자가 제거 또는 파괴되어 있는 것인 미생물.
  16. 청구항 1에 있어서, 상기 미생물은 3-HPA 생산능을 갖는 것인 미생물.
  17. 청구항 1에 있어서, 상기 3-HPA 생산능을 갖는 미생물은 대장균이고, 글리세롤 데히드라타제 (glycerol dehydratase: GDH)를 코딩하는 유전자, 및 글리세롤 데하이드라타제 재활성화효소 (glycerol dehydratase reactivase: GDR)를 코딩하는 유전자가 도입되어 있어 있는 것인 미생물.
  18. 청구항 1의 미생물을 배지 중에서 배양하는 단계;를 포함하는, 아크릴레이트를 생산하는 방법.
  19. 청구항 18에 있어서, 배양물로부터 아크릴레이트를 회수하는 단계를 더 포함하는 것인 방법.
  20. 청구항 18에 있어서, 상기 미생물은 아크릴레이트를 다른 산물로 전환하는 경로를 더 포함하는 것이고, 생산된 아크릴레이트를 다른 산물로 전환하는 단계를 더 포함하는 것인 방법.




KR1020140085356A 2014-07-08 2014-07-08 CoA 아실화 알데히드 데히드게나제 활성이 증가된 신규한 아크릴산 생성 경로를 갖는 미생물 및 이를 이용한 아크릴산 생산 방법 KR20160006030A (ko)

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