KR20150095298A - The manufacturing method of microorganism culture solution Effective Microorganisms - Google Patents

Abstract

The present invention relates to a method for preparing a distilled culture of useful microorganisms. The method, which is used to prepare a distilled culture of useful microorganisms, includes the steps of: heating useful liquid microorganisms to obtain a distilled solution thereof; mixing the distilled solution to a diluted sugar solution; and fermenting the mixed solution at an appropriate temperature. According to the present invention, the distilled culture of the useful microorganisms can be stored for multiple months to two or more years to increase the storability of the conventional useful microorganisms. The useful microorganisms can be applied to the surface of bedding, tools, mirrors, glass windows, tables, flooring, sinks, tiles, electronic appliance, toys, instruments, and cars to show excellent cleaning, disinfection, and deodorization activities. More specifically, the present invention can excellently clean the glass and sink. The present invention can also be applied to garments as a softener in a washing operation to remove odors and static electricity. The present invention also can excellently remove the aphids on plants. Accordingly, the present invention can be utilized as a material for a detergent, cleaner, disinfectant, deodorant, clear coating agent, fabric softener, and aphid removing agent.

Description

Technical Field [0001] The present invention relates to a method for producing a useful microorganism distillation culture,

The present invention relates to a process for producing a useful microorganism distillation culture liquid which can be stored for a long period of time by heating a compound useful microorganism in a liquid phase to obtain a distillate, mixing the distillate with a sugar diluent, Which is characterized in that it can be produced in large quantities. The purpose of the present invention is to describe the content of the production method of the useful microbial distillation culture liquid.

In general, useful microorganisms (EM) are a combination of several microorganisms beneficial to humans among many microorganisms present in nature. Yeast, lactic acid bacteria and photosynthetic bacteria constitute useful microorganisms, and the antioxidant power of fermented products produced by complex coexistence of these microorganisms can be said to be the effect of useful microorganisms. The yeast in the useful microorganism is a microorganism which does not fall into the synthesis of alcohols such as bread, beer, wine and the like, which is a fermentation synthetic organism. It is capable of synthesizing a large amount of amino acids and vitamins and is a food for producing a good microorganism in the soil . The above-mentioned lactic acid bacteria in useful microorganisms have a strong ability to preserve the microorganisms that are easily perishable by the microorganisms that do not fall into the production of yogurt and cheese, and have a major role in fermenting and decomposing organic matter for the first time. The photosynthetic bacteria in useful microorganisms are a kind of arthropods. In food processing, they are partially present in cheese and fisheries (soy sauce made from fish in the tropical regions, a little spicy), and usually like high temperature and high pressure, And is a special microorganism that survives while maintaining a symbiotic relationship on the soil surface. Unlike yeast or lactic acid bacteria, it does not have explosive reproductive ability, but it can be synthesized and fermented by coexistence with these two types.

These complex beneficial microorganisms do not use any synthetic chemicals and are made of 100% natural materials. Representative complex beneficial microorganisms are EM-1, which was developed by Professor Higadero of the Ryukyu University in 1982 The same liquid microorganisms are used in more than 150 countries around the world.

Useful microorganisms were initially used primarily as agricultural materials. Since then, its efficacy and ability have been clarified, and it has been applied to environmental purification, water quality improvement, and public sanitation.

Korean Patent Laid-Open Publication No. 2012-0039092 discloses a feed additive for freshwater eel cultivation using legal sulfur and vegetable lactic acid bacteria, a method for producing the feed additive, and a method for cultivating freshwater eel using the same, and Korean Patent No. 1073887 discloses a sulfur fermentation broth Korean Patent No. 10-1334157 discloses a virgin sulfur produced using a useful microorganism and its use, and a Korean Patent Registration No. 10-1268079 discloses a composition for a food additive using a herb medicine and a method for preparing the same, and Korean Patent No. 10-1114185 discloses a composition for a deodorant and a method for preparing the same.

In addition, its use is expanding to animal feed (Domestic Patent No. 10-0481971), cosmetics (Domestic Patent No. 10-0721119), fabric softener, deodorant, toothpaste and detergent. EM-X is a powerful antioxidant that has been highly concentrated by fermentation of EM fermentation products.

These complex beneficial microorganisms have excellent digestive power and are used as a substitute for digestive agents. However, they are too acidic and difficult to take due to their disgusting taste, and they appear in most of the recipients after a few minutes of taking the medicine. The anal sphincter is not controlled There is a problem that farts become a folk of the people around me.

In addition, useful microorganisms such as EM-1 have a disadvantage in that they have a short preservation ability after being opened for about 15 days to 2 months, spoiled in a relatively short period of time.

In order to solve these problems, the present inventors have studied a distillate obtained by heating a useful microorganism to a high temperature. As a result, the useful microorganism distillate is a photosynthetic microorganism that can withstand high temperatures and high pressures, And it has been confirmed that it has the advantage of excellent preservation ability for 6 years or more in an indoor room below ℃.

In addition, useful microbial distillates are effective for humans when they are consumed in a warmer, less cold environment, excellent digestive power, and various effects such as atopy, athlete's foot, eczema, itching, dandruff and other skin diseases.

In addition, the useful microbial distillate has excellent cleaning ability on the surface of objects including glass, musical instrument, mat, floor, table, tile, kitchen container, stain, painted surface, etc. and excellent deodorization, It is easy to remove by coating force and is excellent in cleaning, disinfection and coating power. In particular, the cleaning power is much superior to that of the useful microorganism before heating, and the transparent microorganism distillate can be sprayed on white walls, bedding, furniture, etc. for the purpose of air purification, sterilization, .

In addition, the useful microbial distillate can be applied to a wide variety of industries, such as an excellent effect for removing aphids from useful microorganisms such as EM-1.

However, the useful microorganism distillate has a problem that the useful microorganisms stick to the surface of the heated soda or the inner surface of the heater during the manufacturing process, and toxic substances such as tar are generated when the microorganisms are continuously heated. Accordingly, there is a problem that the useful microorganism collects and liquefies the useful microbial distillate before producing a substance such as tar to produce a distillate.

Therefore, in order to produce a large amount of useful microbial distillate, it is considered necessary to develop a distiller that inhibits the formation of tar-like substances. Even if a distiller which inhibits the formation of tar-like substances is developed, There is a problem in that the cost is lowered due to the energy source to be consumed.

Disclosure of Invention Technical Problem [8] In order to solve the above problems, it is an object of the present invention to provide a method for producing a useful microorganism distillation culture.

In order to accomplish the above object, the present invention provides a method for producing a useful microorganism, which comprises heating the obtained liquid mixed usable microorganism at a high temperature, collecting and liquefying the generated water vapor to produce a distillate of useful microorganisms, The solution is mixed with the sugar dilution so that it can be cultured, and then fermented at a suitable temperature for a certain period of time to obtain a cultured product of the useful microorganism distillate, that is, a useful microorganism distillation culture. The useful microbial distillation culture liquid is prepared by fermenting the sugar diluent as a food source at a suitable temperature for a certain period of time to produce a useful distillation microbial culture liquid.

According to the present invention, it is possible to produce a large quantity of high-quality useful microorganisms having excellent preservation power, as well as cost reduction by applying a small amount of distillate obtained by heating the liquid multi- It is recognized.

In addition, since a useful microbial distillation culture can be produced in a large amount, it is also an advantageous effect of the present invention that the useful microbial distillation culture fluid has a variety of applications for use as a detergent, a detergent, a bactericide, a deodorant, a coating agent, a fabric softener, something to do.

Hereinafter, the present invention will be described more specifically.

The process for producing the useful microbial distillation culture of the present invention is as follows.

A first step of obtaining a liquid useful microorganism;

A second step of introducing the liquid useful microorganism obtained in step 1 into a distillation tank and heating it until tar is produced to generate steam; And a third step of sending water vapor generated in the second step to a cooling device; And a fourth step of obtaining a liquefied useful microbial distillate in the cooling device of the three stages;

The diluted sugar solution is mixed with at least 1% or more of the useful microbial distillate prepared in the above step 4 at a ratio of at least 1% based on the total weight% of the composition and fermented at a temperature of 20 to 40 ° C for 7 to 30 days to obtain a useful microbial distillate To obtain a useful microorganism distillation culture liquid.

The liquid useful microorganism obtained in the first step according to the present invention is not limited, and may be, for example, Examples of the fermented food include fermented foods such as kimchi, soy sauce, red pepper paste, miso soup, and soy sauce, which are commercially available EM-1, EM-5, EM-X and EM- Fermented soybean paste, fermented soybean paste, fermented soybean paste, fermented soybean paste, fermented soybean paste, fermented soybean paste, and fermented soybean paste. And is not limited as long as it can achieve the object of the present invention such as a fermentation broth in which a useful microorganism in liquid form is present in useful microorganisms that do not die at high temperatures.

The second step according to the present invention is a process in which the liquid useful microorganism obtained in step 1 is heated in a distillation tank until tar is produced to generate steam.

There is no particular limitation on the method of distilling the liquid useful microorganism, and the alcohol fermentation liquor can be added to the distillation tank of distiller, and the distillation can be performed at a temperature of 30 to 80 ° C or a high temperature of 80 to 150 ° C.

Distillation at a middle temperature of 30 to 80 ° C does not produce tar but has a problem that the distillation time takes several days.

On the other hand, the distillation at a high temperature of 80 to 150 ° C can shorten the distillation time, but the useful microorganisms heated at a high temperature have a problem in that microorganisms are adsorbed at the site where heat is generated most in the distillation tank. For example, the inner wall of a distillation tank, particularly a heater or a heater, is adsorbed / stuck to a region where heat transfer is best, and the adsorbed useful microorganism is elasticized from a certain point due to continuous heat transfer, Tar is created.

If distillation is to be performed at a high temperature, the distillation tank of the distiller to be selected may be any one capable of visually confirming the contents therein. It would be more desirable to have a transparent vitreous body.

Of course, the distillation tank for generating steam of the microorganism useful in the present invention is not limited as long as it is a distiller capable of generating steam (of course, it can be selected from various commercially available distillers). However, since tar is generated during the heating of useful microorganisms, a distillation tank is recommended to confirm the condition of the useful microorganisms being heated.

In addition, the liquid multi-functional microorganisms heated in the distillation tank are a combination of dozens of beneficial microorganisms such as yeast, lactic acid bacteria, and photosynthetic bacteria, and these useful microorganisms tend to die at a certain temperature or higher depending on the kind thereof. Lactic acid bacteria are killed at above 65 ℃, yeast is killed at above 85 ℃, but photosynthetic bacteria are not destroyed above 300 ℃. Therefore, the steam generated at 80 ~ 150 ℃ is a distillation solution of photosynthetic bacteria and water, which is distillation solution of useful microorganism.

The third step according to the present invention is a pipeline in which the steam generated from the second stage is sent to a cooling device for collecting and cooling the steam while not diffusing to the outside.

The pipeline is applicable as long as it can preferably be connected to a distillation tank. The pipeline is made of zinc pipe, stainless steel pipe, glass pipe, silicon lake, etc. Especially, when silicon lake is selected as a pipeline, it is easy to handle because it has excellent flexibility and it is resistant to high temperature and toxic due to high temperature. There is no advantage.

On the outside of the pipeline, it is better to have cooling water or a refrigerant body that can cool the hot heat transferred during the transfer of the hot steam.

The fourth step according to the present invention is connected to the pipeline of the third stage to finally collect the vapor conveyed from the third stage and to liquefy the vapor to form a liquid phase.

The container of the fourth stage is preferably made of a material such as stainless steel, glass, porcelain, earthenware, or silicone. It is preferable to exclude containers made of plastic material which generates harmful substances such as environmental hormones at high temperatures.

The outer surface of the container may be provided with a cooling water or a cold medium to liquefy the useful microbial distillate liquid to obtain a useful microbial distillate at a lower temperature.

The useful microbial distillate prepared in this way is transparent and does not corrode for more than 2 years even under the natural environment, and it is confirmed that the microorganism distillate has the advantage of excellent preservation ability for more than 6 years at below 20 ° C there was.

In the fifth step according to the present invention, the useful microbial distillate prepared in step 4 is expanded to obtain a useful microbial distillation culture.

The useful microbial distillate obtained in the above step 4 contains a useful microorganism resistant to high temperatures and thus cultivates / propagates the microbial distillate to prepare a useful microbial distillation culture liquid. The microbial distillate 2 To 20% in a fermenter and mix well. The mixture is fermented at a temperature of 20 to 40 ° C for 7 to 30 days, and the cultured mixture is filtered to prepare a useful microorganism distillation culture.

The water may be any one without any hygienic problems such as distilled water, tap water, deep sea water, purified water, ground water, water softener, and rock water.

The sugar may be at least one selected from sugar, fructose, maltose, lactose, sucrose, honey, sugar, sweet corn, oligosaccharide, fructose, candy, glucose, molasses and raw sugar. The content of the sugar may be in the range of 0.1 to 10%, preferably 0.5 to 5%, and more preferably 0.6 to 3% based on the total weight% of the composition. When the content of sugar is larger than the above range, the useful microorganisms extracted and cultivated are not economical due to too high an acidity or an excessive use amount. On the other hand, when the sugar content is less than the above range, useful microorganisms can not be extracted or cultured from the fermented food There is a problem that the composition is corrupted.

If the amount of the useful microorganism distillate is higher than the above range, there is no problem in culturing, but the economical efficiency is lowered, which is undesirable. Conversely, if the content is small, the useful microorganism distillate may be poorly cultured, have.

In addition to the useful microbial distillate, the sugar diluent may be any one selected from the group consisting of rice flour, rice gruel, rice gruel, skimmed steel, rice rice, rice gruel, rice cake, rice gruel, rice bran, rice confectionery, herbal medicine and vegetables, 2 to 40% of the total weight% can be mixed.

The temperature required for producing the useful microorganism distillation culture liquid is 20 to 40 占 폚, preferably 20 to 35 占 폚, and more preferably 25 to 32 占 폚. If the temperature is higher than the above temperature range, there may be a problem that the fermentation is disturbed in the progress of the fermentation due to heating at a high temperature or an economical loss is required to maintain the high temperature. Conversely, if the temperature is lower than the above range, Or there may be difficulties in progressing fermentation. Therefore, it is preferable to perform in an indoor space without direct sunlight at a preferable temperature.

The fermentation process can be observed with the naked eye. For example, after 36-48 hours from the start of fermentation at 25-35 ° C, gas bubbles are generated in the fermentor, and gas is continuously produced. After 3 to 4 days, To protect it, the germ layer begins to form on the upper surface of the composition. At this time, the composition may be maintained in a state where the lid of the fermentation tank is closed, but the anaerobic fermentation may be continued. However, the lid of the fermentation tank should be opened for at least one time in 1-3 days to evacuate gas or stir once more, When oxygen is supplied and aerobically fermented, more intensive fermentation proceeds. Under these environmental conditions, useful microorganisms in useful microbial distillates are expanded. The fermentation period takes about 7 ~ 30 days.

As described above, the useful microorganism distillation culture that has been cultivated in step 5 above is generally sour like a vinegar but has a slightly strong taste. However, in the process of aging it, the sour in a period of about 1 month, And the taste is smooth and soft, so that it is easier to ingest than the useful microorganisms before distillation.

And the useful microbial distillation culture can be preserved for a long period of time by separating the residue including the by-product by filtering at least once with a filter. When the residue containing the by-product is not filtered, the storage period of the filtrate is lowered, so that the distillation culture of the useful microorganism is preferably separated from the residue containing the by-product by filtration.

It is also preferable to store the filtrate of the useful microorganism distillation culture at a relatively low temperature of 3 to 20 ° C. Under these storage conditions, the useful microbial distillation culture medium is excellent for 2 ~ 4 years.

The present invention also relates to a method for producing a useful microorganism distillation culture solution, wherein the distillation culture solution for useful microorganisms produced by the method for producing the useful microorganism distillation culture has a washing power, a sterilizing power, a deodorizing power, a coating power, an antistatic power, Or the useful microbial distillation culture liquid is used as a material constituting detergent, detergent, disinfectant, deodorant, coating agent, fabric softener, aphid eliminator and the like, or a useful microbial distillation culture liquid for use in the above- .

It can be used as a detergent, a detergent, a deodorant, a coating agent, a fabric softener or the like, or the useful microorganism distillation culture liquid can be used as a detergent, a detergent, a bactericide, a deodorant, a coating agent, A detergent, a disinfectant, a deodorant, a coating agent, a fabric softener and the like are contained in a composition containing a general material in a proportion of 0.1 to 98%, preferably 5 to 20% Cleaning, sterilization, deodorization, coating and the like by spraying, applying, and washing on the surface of an object such as wood, stone, glass, metal, porcelain, earthenware, fiber, plastic or paint. Particularly, the fabric softening agent containing the useful microbial distillation culture liquid may be diluted with an appropriate amount of water in the last rinsing step in laundry washing, or may be contained in the composition constituting the fabric softening agent in a proportion of 0.1 to 10% based on the total weight% By fabricating a fabric softener, it can be used for preventing deodorization and preventing static electricity during washing. In addition, the present invention includes spraying and applying the microorganism distillation culture liquid as it is or diluting it with water to remove aphids produced during plant cultivation, and its application is not limited to those skilled in the art, It is obvious that detergent, detergent, disinfectant, deodorant, coating agent, fabric softener, aphid removal and the like can be suitably implemented by a common manufacturing method.

Hereinafter, the present invention will be described more specifically with reference to Examples and Experimental Examples. However, the technical scope of the present invention is not limited to these.

Example 1

(1) First step for obtaining useful microorganisms

0.5 L of microorganism (EVERMIRACLE), 1 kg of rice bran, 1 g of salt, 1 kg of white sugar, 1 L of ground water and 18 L of ground water purified water are added to a 20 L fermenter and mixed thoroughly for 30 days at a temperature of 28 to 30 ° C, (0.2 mm diameter) formed in the lid of the fermenter for gas discharge. The fermented useful microorganism was used as a filter, and about 15 L of useful yellow microorganism microorganism was obtained.

(2) a second step of producing a useful microbial distillate

10 L of the useful microorganism obtained in the first step is put into a distillate tank and heated at 110 ° C. to vaporize the water vapor generated by the cooling device to collect it in a collecting tank so that strong intense odor and taste peculiar to useful microorganisms disappear, 3 L of a clear and transparent useful microbial distillate was prepared.

(3) a third step of producing a useful microbial distillation culture liquid

0.5 L of the useful microbial distillate prepared in the second step, 18 L of groundwater purified water, and 500 g of white sugar were added to a 20 L fermenter, and the mixture was fermented for 30 days at a temperature of 28 to 30 ° C. (0.2 mm diameter) formed in the lid of the fermentation tank. The fermented broth was filtered with a filter to prepare a useful microorganism distillation broth of about 17L.

Experimental Example

(1) Visual sensory evaluation of storage stability of the conventional useful microorganisms (the formulations obtained in the first step) and the useful microbial distillation culture prepared by the technique of the present invention

Experimental Example 1

The formulations obtained in the first step of Example 1 and the formulations prepared in the third step of Example 1 were each filled with 1.5 L of a transparent PET bottle in an amount of 1 L, and then a lid having a hole with a diameter of about 0.2 mm was placed in the opening And after leaving for about 10 months in a sunny vinyl house installed on the roof of a building, the sensory evaluation result of comparing the degree of corruption by visually observing the time when a blue mold was formed in the bacterium after the formation of the bacterium on the upper surface of each formulation Table 1 shows the results.

   Form      When the blue fungus is generated (as of the start date of the experiment) The formulation obtained in the first step of Example 1                   67 to 77 days The formulation prepared in the third step of Example 1
Not observed during the experiment

Experimental period: From the beginning of May to the end of February of the following year (when the midday temperature reaches 45 ℃ in summer or falls more than 20 ℃ in winter)

Observations: Each formulation was observed at least once on days 1-10.

<Result>

As a result of comparing the degree of corruption by visually observing the time when the blue mold was formed on the upper layer surface of the respective formulations after the formation of the fungi, the results obtained in the first step of Example 1 were 67 ~ 77 days, but it was not observed in the formulations prepared in the third step of Example 1. This indicates that the formulations prepared by the technique of the present invention are superior to the conventional useful microorganisms in terms of product retention, storage stability.

(2) Sensory test for dishwashing power

Experimental Example 2

The formulation prepared in the third step of Example 1 and water were mixed in a small atomizer bottle at a ratio of 1: 2 and mixed thoroughly. The mixture was sprayed onto the surface of each dish having food thereon and then the two methods were performed , The first group rubbed the tableware with a rubbing cloth and rinsed with tap water, and the second group rubbed the surface of the tableware with a clean tissue paper. Participants were one housewife in their 20s and three housewives in their 50s. The results of the sensory evaluation of the washing performance of the formulation prepared in the third step of Example 1 as a dishwashing detergent are shown in Table 2 below.

Form
           Sensory measurement of dishwashing power     The first group         The second group The formulation prepared in the third step of Example 1            4                4

1) The number is the average of 10 experiments

2) Acceptance criteria; 5; Very good, 4; Good, 3; Usually, 2; Poor, 1: Very bad

<Result>

The formulation prepared in the third step of Example 1 was somewhat less than the general chemical detergent, but the dishwashing agent was washed as in the first group and the second group It is considered to be suitable as a detergent, and the rating is also excellent as shown in Table 2. Especially, it is considered that it is suitable to wash dishware without water in a place where it is difficult to obtain water (for example, when a mountain or a tap water is splashed), thereby reducing the pollution source. Furthermore, the formulations prepared in the third step of Example 1 are capable of disinfecting and sterilizing germs that may remain in the dishes after washing due to sterilizing power, coating power, deodorizing power, etc., And deodorizing ability to remove the back.

(3) Sensory evaluation of deodorization ability of odor of pet excrement

Experimental Example 3

The formulation prepared in the third step of Example 1 was filled in a small spray bottle, and then the dog (11-year-old Martijs male) was examined for urine or feces in the floor of the floor, the floor of the floor, First, the formulation prepared in the third step of Example 1 is sprayed at least once with a sprayer at a place where the slurry is removed from the tissue paper, and then the tissue is wiped clean with a tissue paper, a dry cloth, a wet cloth or the like. Participants were 1 in their 20s and 5 in their 40s and 50s housewives. Table 3 shows the results of sensory evaluation in which the removal performance (deodorizing power) of the excrement of the pet was measured by the distillation culture of the useful microorganism of Example 1.

 Form      Sensory evaluation of odor elimination ability measurement of excreta of pet The formulation prepared in the third step of Example 1                         5

1) The number is the average of five experiments

2) Acceptance criteria; 5; Very good, 4; Good, 3; Usually, 2; Poor, 1: Very bad

<Result>

As a result of measuring the odor removing power (deodorizing power) of the pet's feces on the formulations prepared in the third step of Example 1, the formulations prepared in the third step of Example 1 were sprayed on the smell of pet feces, It was found that the deodorizing power was never lowered as compared with the general chemical deodorant, and the rating was excellent as shown in Table 3 as well. Furthermore, the formulations prepared in the third step of Example 1 were sterilized and coated, and the germs remaining in the floor of the floor, the floor of the floor and the floor of the floor of the toilet were decomposed and sterilized, It makes it smoother.

(4) Sensory evaluation of detergency of laundry

Experiment 4

The infant clothes worn were dipped for 8 hours in a diluted water diluted at a ratio of 1:20 with the formulation prepared in the third step of Example 1, cleaned with tap water, dried naturally, The undergarment of white color was immersed in the diluted water diluted at a ratio of 1:50 with the formulation prepared in the third step of Example 1 for 6 hours, rinsed with a washing machine, and dehydrated sequentially, Table 4 shows the results of sensory evaluation of the smell removal ability and the visual measurement of the washing power for the dry second laundry.

division
  Visual measurement of cleaning power   Olfactory measurement of deodorizing power   The first laundry  The second laundry   The first laundry    The second laundry The formulation prepared in the third step of Example 1                        5                        5                        5                         5

1) The number is the average of three experiments

2) Acceptance criteria; 5; Very good, 4; Good, 3; Usually, 2; Poor, 1: Very bad

<Result>

The visual measurement of washing power and the sensory evaluation of deodorizing power on the formulations prepared in the third step of Example 1 were excellent as shown in Table 4.

In particular, in the case of infant clothes, it is common that the smell of laundry laundered by using a chemical detergent is not completely disappeared. In the first laundry laundered by the formulation produced in the third step of Example 1, It was very good because it did not remain. Also, the second laundry was washed more clearly in white. This is because the contamination source of the formulation prepared in the third step of Example 1 was cleanly decomposed and removed.

(5) Sensory test for prevention of static electricity of laundry

Experimental Example 5

In order to prevent static electricity when washing laundry such as clothes, clothes, etc., in the last washing process, the distillation culture solution of the useful microorganisms finally prepared in Example 1 is applied 20 to 100 mmL at the maximum amount of water based on 10 kg of laundry, The results of the sensory evaluation of the formulation prepared in the third step of Example 1 were carried out by the above-mentioned inspiration method, as shown in Table 5 .

 Form      Anti-static measurement sensory test for laundry garments The formulation prepared in the third step of Example 1                         5

1) The number is the average of five experiments

2) Acceptance criteria; 5; Very good, 4; Good, 3; Usually, 2; Poor, 1: Very bad

<Result>

Results of Measurement of Antistatic Preventive Measures of Laundry for Formulations Prepared in Third Step of Example 1 The formulations prepared in the third step of Example 1 were administered at the time of rinsing laundry, and the rinsing and dehydration processes of the washing machine were sequentially performed The dried clothes were heated with a hair dryer and then contacted with the hair. No static electricity was generated, and the rating was excellent as shown in Table 5.

In addition, the dried laundry rinsed with the formulation prepared in the third step of Example 1 was coated with the surface of the fiber by the coating force of the formulation prepared in the third step of Example 1, so that the clothes were softly used It is thought that it can become.

(6) Sensory evaluation on the removal ability of aphids

Experimental Example 6

For removing the aphids on the cultivated leaves, the first group obtained by spraying the useful microbial distillation culture solution finally prepared in Example 1 with a small sprayer on the leaves without leaves, the formulation prepared in the third step of Example 1, and the water Were mixed in a ratio of 1: 2 and divided into a second group in which the leaves were sprayed with a small atomizer. The results of sensory evaluation of the formulation prepared in the third step of Example 1 for the removal of aphids were as shown in Table 6 .

Form
           Sensory evaluation for aphid elimination     The first group         The second group The formulation prepared in the third step of Example 1           5            4

1) The number is the average of three experiments

2) Acceptance criteria; 5; Very good, 4; Good, 3; Usually, 2; Poor, 1: Very bad

<Result>

As shown in Table 6, the results of the measurement of the aphid elimination result of the formulation prepared in the third step of Example 1 are excellent in the first group and good in the second group, which is suitable as an aphid remover.

(7) Sensory test for water removal power (coating power)

Experimental Example 7

After removing the impure substances on the surface of the sink by using a scrubber, wash the dishwasher with diluted kitchen chemical cleaner (manufacturer: CJ Lion, product name: green green tea dish) with water, remove water from the sink surface with a dry dish, The first method of natural drying with good ventilation for 1 hour, the chemical washing agent (manufacturer: CJ Lion, product name: green green tea washing dish) diluted with water is wiped off with a wringer to remove impurities on the surface of the sink, After mixing the formulation prepared in the third step of Example 1 with water in a ratio of 1: 2, the mixture was sprayed on the surface of the sink with a sprayer, the water on the surface of the sink was removed with a dry cloth, The results of the sensory evaluation of the formulation prepared in the third step of Example 1 for the removal of water droplets are shown in Table 7 below And the like.

Form
           Sensory evaluation for water removal power     First Method         Second Method The formulation prepared in the third step of Example 1           2            5

1) The number is the average of three experiments

2) Acceptance criteria; 5; Very good, 4; Good, 3; Usually, 2; Poor, 1: Very bad

<Result>

As shown in Table 7, the first and second methods were found to be inferior and excellent, and thus the useful microbial distillation culture liquid prepared by the technique of the present invention showed excellent water-removing ability by the coating power and is suitable for removing water droplets .

Although specific embodiments of the method for producing the useful microbial distillation culture liquid belonging to the present invention as described above are exemplified and described above, the present invention is not limited to the above-described embodiments, examples and experimental examples, It will be apparent to those skilled in the art that various other changes and modifications can be made without departing from the spirit and scope of the present invention, It should belong to the scope.

The useful microbial distillation culture produced by the technique of the present invention can be cultivated in a large amount in a comparatively short period of time, and the useful microbial distillation culture is utilized as a detergent, a detergent, a bactericide, a deodorant, a coating agent, a fabric softener, It is possible to produce products such as detergents, cleaning agents, disinfectants, deodorants, coatings, fabric softeners and aphids, which contain the useful microbial distillate culture liquid, so that they can be strategically used for targeting niche markets in related fields.

Claims (4)

A first step of obtaining a liquid useful microorganism; A second step of introducing the liquid useful microorganism obtained in step 1 into a distillation tank and heating it until tar is produced to generate steam; And a third step of sending water vapor generated in the second step to a cooling device; And a fourth step of obtaining a liquefied useful microbial distillate in the cooling device of the three stages; The diluted sugar solution is mixed with at least 1% or more of the useful microbial distillate prepared in the above step 4 at a ratio of at least 1% based on the total weight% of the composition and fermented at a temperature of 20 to 40 ° C for 7 to 30 days to obtain a useful microbial distillate To obtain a useful microbial distillation culture liquid. The method for producing the distillation microorganism culture of useful microbes according to claim 1, The method according to claim 1,
In the fifth step, the sugar is at least one selected from the group consisting of sugar, fructose, maltose, lactose, sucrose, honey, sugar, sweet corn, oligosaccharide, fructose, raw sugar, candy, glucose, molasses and raw sugar. Way. A useful microbial distillation culture, characterized in that the useful microbial distillation culture is prepared by the method for producing a useful microbial distillation culture according to any one of claims 1 to 2. 4. The useful microbial distillation culture liquid according to claim 3, wherein the useful microbial distillation culture liquid is composed of any one of a detergent, a detergent, a bactericide, a deodorant, a coating agent, a fabric softener, and aphid removal.