KR20150083225A - Livestock assorted feed with enhanced nutrients and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a method for manufacturing livestock assorted feed with enhanced nutrients by using liquid yeast fermented liquor which increases productivity and preference.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a nutritional composition-

The present invention relates to a compounded feed for fortification fortified with nutrients by using a liquid yeast culture as a feed additive and a method for producing the same.

Yeast is a single-celled microorganism having a eukaryotic cell structure, and is generally referred to as a fungus that proliferates by germination. In general, yeast contains essential amino acids, vitamin B group, and minerals, which are the nutrients of the cell itself, and the digestive enzymes and metabolites released during yeast growth and autolysis increase animal productivity and digestibility The minerals formed in the metabolism of yeast are very chelated minerals and have very high bioavailability.

Thus, yeast is also used as a cheap fat and protein source in ruminant feed additives. The yeast used as a feed additive for ruminants is an unripe growth factor and improves the palatability of the feed with flavor substances such as glutamic acid. Since the cell wall of yeast is composed of high unit compounds such as mannan and glucan and has a strong affinity with oxygen, it is possible to improve fermentation environment such as removal of residual oxygen in rumen and pH, It has been known to promote microbial degradation and growth. In order to keep the animals healthy and to produce efficiently, beneficial microorganisms must be proliferated continuously. Yeast promotes the growth of lactic acid bacteria in the intestines of animals, reduces the number of harmful bacteria to improve the intestinal environment, (Cartwright et al., 1986; Rose, 1980; Shin et al., 1990).

In particular, the effect of adding live yeast can be easily found in various literature data. In particular, in the AAFCO, 1989, the definition of raw yeast culture is defined as "a culture medium containing yeast and yeast Is a product that is dried by a method in which the fermentation ability of the yeast is preserved, and the composition of the medium must be specified. " The active ingredient is divided into raw yeast cells, medium components and metabolic products. The yeast cells secrete various digestive enzymes into the medium to decompose them into low molecular substances (monosaccharides, amino acids, fatty acids) capable of absorbing the medium components, And the cells proliferate and secrete metabolic products such as various enzymes, vitamin amino acids, unknown growth factors, organic acids and yeast flavor.

When feeding high diets to high nutrition and nutrition supplements, the pH of the rumen decreases and the microorganism is destroyed, resulting in rumen and acidosis. Such ruminal and acidosis damage the gastric mucosa of the livestock, resulting in the liver abscess and the simultaneous occurrence of various metabolic diseases, resulting in a significant decrease in the productivity of livestock. Therefore, feed additives that help rumen fermentation are needed when overfeeding the compound feed. The yeast culture contains lactic dehydrogenase (D-lactic dehydrogenase) which can utilize D-lactic acid, (Jarrett, 1985), which has a cell wall that can be used to prevent pH drop.

Yeast are mostly used as a feed additive when they are mixed with total mixed ration in the farm. Generally, yeast used as a feed additive is killed by yeast due to high temperature and high pressure molding do.

In order to solve this problem, methods have been developed in which liquid yeast is transformed into a solid state using an excipient or coated in a solid state to withstand high temperature and high pressure conditions.

Korean Patent Registration No. 10-0410830 discloses a method for culturing lactic acid bacteria, yeast and Bacillus subtilis, culturing them by adsorbing and fixing them on a zeolite and a degreasing mixture, drying them, compounding them, and crushing them to prepare a microorganism preparation for feed addition .

Korean Patent Publication No. 10-1115306 discloses a method for producing a high-concentration liquid probiotic seed bacterium from a liquid medium containing jujube as a main component and then re-fermenting the seed bacterium in a solid medium containing jujube as a main component, And a method for producing the same.

Korean Patent Registration No. 10-1252132 discloses a method of mixing a feed additive obtained by drying a culture obtained by inoculating a strain of Bacillus genus, lactic acid bacteria and yeast in a mixed medium obtained by mixing liquid chlorella and a solid medium, To a method for producing a non-dairy cow feed.

However, despite these efforts, it has been confirmed through separate experiments that the number of yeast cells after the preparation of the compound feed containing the yeast-containing feed additive can be measured by high temperature and high pressure process Most of the yeast were killed in the final mixed diet.

As a result of repeated studies to overcome the above-mentioned problems, the present inventors have found that, after a feed manufacturing process in which a high-temperature and high-pressure process is present, the liquid yeast culture liquid is sprayed using a liquid spray device, It was confirmed that more yeast cells were alive and the present invention was completed.

Accordingly, it is an object of the present invention to provide a method for preparing a fortified dietary composition using a liquid yeast culture as a feed additive for enhancing nutritional components of a fortified dietary supplement.

It is another object of the present invention to provide a fortified dietary supplement fortified with the nutritional components produced by the above-mentioned production method.

Therefore,

(a) inoculating and culturing yeast in a liquid medium to prepare a liquid yeast culture;

(b) selecting and mixing the raw material for compound feed;

(c) molding the blended raw material into pellets through a pellet forming machine;

(d) cooling the shaped pellets;

(e) spraying the culture liquid obtained in step (a) onto the outer surface of the cooled pellet; And

(f) cooling the sprayed pellet of the liquid yeast culture liquid;

Wherein the nutritional component is added to the nutritional composition.

The present invention also provides an axial feed composition fortified with the nutritional components produced by the above-described method.

According to the production method of the present invention, since a greater number of yeasts can survive in the compounded feed, it is possible to provide a fortified compounded feed having enhanced nutritional components.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a view showing a liquid microbial agent spray spray facility. FIG.
Fig. 2 shows changes in pH with time in the artificial rumen test.
Fig. 3 shows changes in the digestibility of organic matter according to the incubation time in the artificial rumen test.
Figure 4 shows changes in volatile fatty acids in the rumen according to incubation time in the artificial rumen test.
Fig. 5 shows the test results (change in flow rate) of the mixed feed feeding specification including the liquid yeast culture liquid.

Hereinafter, the present invention will be described in more detail.

As a first aspect of the present invention,

(a) inoculating and culturing yeast in a liquid medium to prepare a liquid yeast culture;

(b) selecting and mixing the raw material for compound feed;

(c) molding the blended raw material into pellets through a pellet molding machine using warming / pressing;

(d) cooling the shaped pellets;

(e) spraying the culture liquid obtained in step (a) onto the outer surface of the cooled pellet; And

(f) cooling the sprayed pellet of the liquid yeast culture liquid;

Wherein the nutritional component is added to the nutritional composition.

The liquid medium may be any medium that can be used for culturing yeast, and it is preferably a medium containing 3 to 13% by weight of molasses, 1-5% by weight of glucose and 0.1-0.2% by weight of NaCl, It does not. The content of the components in the medium can be suitably adjusted by those skilled in the art. In addition, a medium sterilized at high temperature may be preferable in order to suppress the growth of germs.

Yeast cells inoculated into the liquid medium may be inoculated at 1-5 wt% with respect to the whole medium, but are not limited thereto. The yeast may be a known strain. Saccharomyces cerevisiae may be used, but is not limited thereto.

Generally, yeast which can be used as feed additives are aerobic and tuberous anaerobes and can grow under both aerobic and anaerobic conditions. They usually breed well at pH of 4.0-6.5 and their optimum growth temperature is 25 ℃ -35 ℃ to be. In one embodiment, step (a) may be preferably carried out by inoculating yeast, stirring at 35 DEG C for 15 minutes, and then incubating at 37 DEG C for 3 days.

The culture yeast culture obtained in the step (a) preferably contains yeast cells of 5 x 10 ⁷ - 1 x 10 ⁹ cfu / g.

In the step (b), the compound feed feedstock may be any of conventional feedstuffs necessary for the production of normal feedstuffs used in fowl, for example, early and / or mid-term feedstuffs.

In the step (c), the produced pellets for compound feed are preferably produced using a double pelletizer or a single pelletizer. Since the hardness is higher and the pellet is solidified to form a high ruminal permeation rate, More preferable.

In the step (d), the molded pellet is preferably cooled to room temperature to remove moisture.

In the step (e), it is preferable that the culture liquid yeast culture produced in the step (a) is sprayed by a liquid spray device.

In the step (e), it is preferable that the liquid yeast culture solution prepared in step (a) is added in an amount of 0.05-0.5% of the total compound feed.

In the step (f), the pellet sprayed with the liquid yeast culture liquid is preferably cooled to room temperature by using a cooling machine at 18 to 25 DEG C to remove moisture.

As a second aspect of the present invention, the present invention also provides an axial feed composition fortified with the nutritional component produced by the above-mentioned production method.

Since fibrinous bacteria in the rumen utilize the fatty acids produced by the lipolysis of the yeast in the yeast culture as nutrient sources, the feeding of the yeast cultures increases the number of fibrinolytic bacteria in the rumen and increases the production of total volatile fatty acids in the rumen. The excessive feeding of diets to lactating mothers increases the amount of propionic acid and the amount of acetic acid to be produced. In the highly dependent condition of diets, the feeding of yeast culture increases the production of lactic acid, which is a precursor of milk fattening milk.

The compounded diets according to the present invention were found to increase the digestibility of organic matter in the rumen and increase the amount of volatile fatty acid production in the rumen (Figs. 2 to 4), and the flow rate increased due to the growth of the fibrinolytic bacteria (Table 7 and Fig. 5).

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, these examples and experimental examples are only for the sake of understanding the present invention, and the scope of the present invention is not limited in any sense in any sense.

[ Example ]

Example  1: Production of liquid yeast culture liquid (culture liquid 500 ml  standard)

25 ml of molasses, 10 g of glucose and 0.5 g of NaCl were added to an Erlenmeyer flask, and the total volume was adjusted to 500 ml with distilled water. This was sterilized in a sterilizer at 121 DEG C for 20 minutes. After completion of the sterilization, the mixture was cooled to 35 DEG C, and 5 g of yeast cells were added thereto while maintaining 35 DEG C. The mixture was stirred for 15 minutes in an agitator, followed by incubation at 37 ° C for 3 days.

The cultured yeast culture was subjected to microscopic examination to produce a yeast culture medium of 5 x 10 ⁸ cfu / g.

Example  2: Evaluation of optimized medium conditions for the production of liquid yeast culture

In order to verify the medium conditions capable of culturing the largest amount of yeast, the content of the culture medium was changed as shown in Table 1, and the number of yeast cells in the yeast culture was measured.

Medium component Medium component number 1 No.2 number 3 No. 4 5 times % glucose 2 4 4 2 2 molasses 5 10 5 10 5 leaven One 2 One One 2 NaCl 0.1

As shown in Table 1, the culture results of the yeast cultured by changing the content of the medium components were as shown in Table 2 below.

The number of yeast cells in the culture medium of the yeast by changing the content of the medium component division number 1 No.2 number 3 No. 4 5 times Number of yeast cells (cfu / g) 5.0 x 10 ⁸ 4.0 x 10 ⁸ 3.8 x 10 ⁸ 1.4 x 10 ⁸ 1.0 x 10 ⁸

Example  3: Liquid yeast The culture medium  Production of fowl formula

After selecting the raw material for the nutrient, weighed to the weight.

It has been reported that corn, wheat bran, wheat bran, wheat bran, wheat bran, wheat bran, wheat bran, And 0.2% mineral minerals were blended together for 5 minutes to have a composition of 11.22% moisture, 18.14% crude protein, 4.53% crude fat, 6.24% crude fiber, 6.01% ash, ADF 11.23% and NDF 23.54% The compound feed was prepared.

The blended raw materials were molded into pellets through a pellet molding machine under pressure at a temperature of 80 DEG C / 1.5 BPS. The molded pellet was cooled to room temperature to remove moisture, and then the liquid yeast culture solution prepared in Example 1 was sprayed onto the outer surface of the pellet cooled to room temperature with a liquid spray device (FIG. 1). Cooling was performed to remove water from the mixed feed in which the liquid yeast culture was sprayed.

The yeast cell count of the liquid yeast culture prepared in Example 1 was 5 x 10 ⁸ cfu / g, and 0.5% yeast was added to the yeast culture. The yeast count in the diets was 1 x 10 ⁶ cfu / g.

Viability of Yeast in Mixed Diets over Time Elapsed time 0h 12h 24h 48h 72h 96h 120h 168h 360h Liquid treatment
Number of yeast cells (cfu / g) 1 x 10 ⁶ 1 x 10 ⁶ 8 x 10 ⁵ 8 x 10 ⁵ 8 x 10 ⁵ 6 x 10 ⁵ 3 x 10 ⁵ 1 x 10 ⁵ 1 x 10 ⁵ Solid processing
Number of yeast cells (cfu / g) Death

As shown in Table 3, the yeasts in the compounded feed prepared by the method according to Example 3 were significantly higher than those in the compound feeds produced by mixing the existing solid yeast cells in the compounded feed, Activity and milk fat increase, and the survival and survival rate were higher than those of the conventional methods.

Example  5: Verification of the viability of yeast strains in livestock feed using liquid culture of liquid yeast

In order to investigate the viability of yeast in diets according to seasonal temperature changes (high temperature - summer, low temperature - winter season) when feeding on farm (field) Respectively.

Yeast survival verification tests were conducted according to the temperature conditions (high temperature-50 ° C, low temperature-0 ° C) of the compound feed prepared in Example 3, and the results are shown in Table 4 below.

Viability of Yeast in Mixed Feed according to Storage Temperature Number of yeast cells (cfu / g) 0h 24h 96h 192h 360h Low temperature (0 ℃) 1.0 x 10 ⁶ 2.2 x 10 ⁵ 1.6 x 10 ⁵ 1.6 x 10 ⁵ 1.0 x 10 ⁵ High temperature (50 ℃) 1.0 x 10 ⁴ 2.8 x 10 ² Death

As shown in Table 4, it was found that yeast weak in heat had a high survival rate under low temperature conditions, but the yeast was killed after a certain period of time under high temperature conditions, so that product storage in the summer should be avoided from direct sunlight and stored in the shade .

Example  6: Artificial rumen experiment of fowl feed with liquid yeast culture

The artificial rumen experiment was carried out using the compound feed prepared in Example 3 as follows.

Representative samples were collected from 50% of the feedstuffs (straw) generally consumed at the farm (field) and 50% of the compounded feed prepared in Example 3.

A sample weight of about 0.5 g per sample was placed in a 125 ml serum bottle and the container was stored in a 39 ° C incubator. The first five chemicals in Table 5 were mixed with 500 ml of distilled water in a round bottom flask, stirred until dissolved, and adjusted to volume to prepare ruminal artificial saliva. Just prior to use CaCl ₂ was added and the solution was kept at 39 ° C until pH = 8.0. The artificial saliva was sterilized at 121 ° C for 20 minutes and stored in a 39 ° C incubator (McDougall, 1948).

Artificial saliva component ingredient Content (g) NaHCO ₃ 9.80 Na ₂ HPO ₄ .7H ₂ O 7.00 KCl 0.57 NaCl 0.47 MgSO ₄ .7H ₂ O 0.12 CaCl ₂ 0.04

In order to collect the gastric juice, a warm water bottle with carbon dioxide was prepared in advance, and the gastric juice of the Holstein beef cattle was collected with 8 layers of gauze, stored in a thermostat at 39 ° C, and put into the anaerobic state Respectively. The gastric juice collected on the gauze was again used with an 8-fold gauze.

The temperature of the anaerobic dispenser was raised to 330 ° C and copper was reduced using hydrogen. The prepared artificial saliva and gastric juice were mixed at a ratio of 4: 1, and carbon dioxide was continuously supplied. The serum container stored in the incubator was taken out, and the prepared inoculum was inoculated into each serum container in an amount of 50 ml.

Carbon dioxide from the anaerobic digestion device was injected into each prepared serum container for 5 seconds, and the anaerobic condition was maintained using a prepared rubber stopper and an aluminum cap (20 mm seal). The cells were cultured in a 39 ° C incubator.

Samples were taken from each serum container at 0, 3, 6, and 24 hours.

According to the artificial rumen test method above, experiments were carried out by dividing into the group of no addition of liquid yeast (control group) and the group of addition of liquid yeast culture prepared in Example 3 (treatment group).

The results of the artificial rumen experiment are shown in Figs. 2, 3 and 4. The results show that when the yeast in the compound feed is added, the pH in the rumen increases, the digestibility of organic matter in the rumen increases with the increase of the fibrin digestibility, Increase in the number of workers.

Example  7: Using the culture of liquid yeast culture, Milking  Pay exam

The compound feed prepared in Example 3 was fed to the field dairy farm to observe the change of the feed rate.

The experimental farm was located in Paju City, Gyeonggi Province, and 27 specimens (average weight 630kg) of Holstein milking were reported as test animals and specimens were tested for 31 days. Table 6 shows the average results of the specification test in which the compound feed prepared by adding the liquid yeast additive of Example 3 at a ratio of 1: 1 was fed during the test period of 31 days while feeding the compound feed not containing the existing liquid yeast yeast.

In addition, when the yeast in the compounded feed was added, the productivity (flow rate) was increased due to the growth of the fibrinolytic bacteria, and compared with before / after the specimen test, the flow productivity was increased by 1.6 kg.

Variation of flow rate before and after feeding of fed-batch feed with liquid yeast culture division Salary before
(1 day) After salary
(31 days) Flow rate (kg / two / day) 26.9 28.5

Claims (10)

(a) inoculating and culturing yeast in a liquid medium to prepare a liquid yeast culture;
(b) selecting and mixing the raw material for compound feed;
(c) molding the blended raw material into pellets through a pellet forming machine;
(d) cooling the shaped pellets;
(e) spraying the culture liquid obtained in step (a) onto the outer surface of the cooled pellet; And
(f) cooling the sprayed pellet of the liquid yeast culture liquid;
≪ / RTI > wherein the nutritional component is added to the nutritional composition. The method according to claim 1,
Characterized in that the liquid medium comprises 3-13% by weight molasses, 1-5% by weight glucose and 0.1-0.2% by weight NaCl. The method according to claim 1,
Characterized in that the yeast is added in an amount of 1-5% by weight relative to the total medium. The method according to claim 1,
Wherein the liquid yeast culture broth contains 5 x 10 ⁷ cfu / g - 1 x 10 ⁹ cfu / g of yeast. The method according to claim 1,
Wherein said culturing is carried out at 37 DEG C for 3 days. The method according to claim 1,
In the step (d), the molded pellets are cooled to room temperature to remove moisture. The method according to claim 1,
In the step (e), the liquid yeast culture liquid prepared in the step (a) is sprayed by a liquid spray device. The method according to claim 1,
Wherein the liquid yeast culture liquid prepared in step (a) is added in an amount of 0.05 to 0.5% of the total compound feed in step (e). The method according to claim 1,
In the step (f), the pellet sprayed with the liquid yeast culture liquid is cooled to room temperature using wind at 18 to 25 ° C. A compounded feed for fortification fortified with a nutritional component prepared according to any one of claims 1 to 9.

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