KR20150077214A - Liquid crystal cosmetic composition having similar skin lipid composition and containing glucosylceramide, and preparation method thereof - Google Patents

Abstract

Provided are a cosmetic composition for protecting the skin comprised of a composition similar to an intercellular lipid of a skin barrier containing ceramide, glucosylceramide, and sphingolipid, and a manufacturing method thereof. According to the present invention, the cosmetic composition is manufactured as a composition of a liquid crystal shape using a component of a skin lipid existing on the outer skin. By including the nanoliposomized glucosylceramide as an active component, the present invention enables to easily penetrate through the skin as well as maximizing skin penetrability and moisturizing ability since the glucosylceramide is able to be reached to an inner skin layer.

Description

TECHNICAL FIELD [0001] The present invention relates to a liquid crystal cosmetic composition having a skin-like lipid composition containing a saccharide-containing ceramide,

The present invention relates to a composition containing ceramide, glucosylceramide, and Sphingolipid to form a liquid crystal when applied to a formulation, having a structure and composition similar to the stratum corneum and intercellular quality of human skin, And a skin penetration ability, and a method for producing the same.

Since dry skin causes various skin diseases, it is the basis of skin protection to keep the skin moisturized. In addition, the skin is exposed to a number of external microorganisms, and it is known that the skin composition is in the stratum corneum for maintaining the skin in a normal state (Normal Bacterial Flora). The main component of the stratum corneum of the skin is dead cells, consisting of keratin, a hard support such as brick, and intercellular lipids, which attach the support to the cement like cement, It is known that it acts as a variety of physiological protective membranes such as skin protection from external harmful factors. In order to develop the most desirable skin application product for maintaining a healthy skin condition, it is necessary to fully understand the structure of the skin and the biomembrane composition of the skin cells and their physical properties.

Glucosylceramide, which is present in the tissues of animals and plants, is a structure in which saccharides such as glucose, galactose, and lactose bind to ceramide. The sugarcereamides have superior solubility to water and ethanol as compared to simple ceramides because sugar is combined. In addition, sugar-bound ceramide has excellent feeling and excellent moisturizing effect compared to ceramide, and various effects such as anti-inflammation, atopic improvement, whitening effect, and wrinkle improvement have been reported. In particular, simple ceramides are difficult to apply to cosmetics due to their solubility problems, so that many products related to formulations have not been introduced yet. Even if they are commercialized, stability of formulation is unstable. On the other hand, sugarcereamide has a high solubility in water and ethanol, so it is relatively easy to apply cosmetics and it is known that its ability to capture moisture is several orders of magnitude higher than ceramide.

The saccharide ceramide is present in small amounts in all animal tissues such as neurons such as neurons, spleen and red blood cells, and also constitutes 4% of the total epidermal lipid mass as a main constituent of skin lipids, and is one of the essential elements constituting the lamellar phase . The lamellar phase is a cell organelle that stores lipids released from the keratinocyte (Keratinocyte). By using these storage organs, the skin can quickly regulate the level of ceramides by rapidly responding to changes in the external environment, thereby maintaining proper moisture and skin barrier function of the skin. Studies by the Jenneman group have shown that glyceraldehyde deficiency in the epidermal layer induces an irregular arrangement of the lamellar phase of the stratum corneum and extracellular lipids using transgenic mice. In addition, the absence of the glucosylceramide-β-glucosidase resulted in the conversion of sphingomyelin into a reservoir of ceramide, which confirmed the lack of certain types of ceramides required to maintain skin barrier function ( J. Bio . Chem . 2007, 119, 417-423). This is the basis for demonstrating that sugar ceramides play an important role in producing certain types of ceramides required to maintain skin barrier function.

Meanwhile, Korean Patent Laid-Open Publication No. 2001-0011241 discloses a conventional skin protection composition having the same lipid composition and structural properties as a biological membrane existing in a human intercellular region. Such prior art has focused not only on a method for preparing a composition based on ceramide, cholesterol and fatty acid but also because it contains a simple ceramide which is difficult to apply to cosmetics due to its solubility problem. Therefore, There arises a problem that the stability and moisture retention of the formulation are lowered and the stability of the formulation after manufacture is lowered. Also, since the composition was prepared by simply mixing materials similar to the lipid composition of the skin, there was a limit to the ability to be related to skin penetration and skin moisturizing.

Disclosure of the Invention The present invention has been conceived to overcome and solve the above problems, and it is an object of the present invention to provide a multi-layer liquid crystal which is a multi-layer liquid crystal using ceramide, cholesterol, fatty acid, phospholipid and phytosphingosine, It has been noted that liposomal saccharide ceramides having an added composition as an active ingredient can enhance skin lipid membrane strengthening and moisturizing ability, facilitate skin penetration, and cause synthesis and fractionation of ceramides in the skin.

Accordingly, it is an object of the present invention to provide a cosmetic composition which has the aforementioned composition and maximizes skin permeability and skin moisturizing power, thereby improving skin dryness, atopic dermatitis, and moisturizing power, and a cosmetic comprising the Maltecross liquid crystal.

It is another object of the present invention to provide a method for producing the above-described cosmetic composition by using a liquid crystallization method of a saccharide-containing skin lipid.

In order to achieve the above object, the present invention provides a liquid crystal forming composition comprising (A) a water-soluble substance portion and a liquid crystal lipid portion; And (B) a saccharide-containing liposome composition, wherein the saccharide-containing liposome composition is contained in an amount ranging from 5.0 to 30.0% by weight based on the total weight of the cosmetic composition, There is provided a liquid crystal cosmetic composition having a similar lipid membrane.

According to a preferred embodiment of the present invention, the saccharide-containing liposome composition (B) comprises 0.015 to 2.0 wt% of sugar ceramide, 1.0 to 15.0 wt% of phospholipid, 1.0 to 50.0 wt% of polyhydric alcohol, And a balance of water satisfying 100 wt% of the composition.

According to another preferred embodiment of the present invention, the liquid crystal forming composition (A) comprises a liquid crystal lipid part and a water soluble substance part, wherein the liquid crystal lipid part comprises 0.15 to 4.0% by weight of ceramides, 0.05 to 2.0% by weight of cholesterol, 0.05 to 2.0% by weight of phytosphingosine, 0.5 to 10.0% by weight of a phospholipid and 1.0 to 20.0% by weight of an oil, wherein the water-soluble substance portion comprises a residual amount satisfying 3.0 to 20% by weight of the polyhydric alcohol and 100% Of water.

The cosmetic composition of the present invention may be used for skin protection and skin moisturization by forming a skin-like lipid membrane, and it is preferable to form a Maltecross liquid crystal when the final formulation is applied.

Preferably, the cosmetic composition is a liposome-containing saccharide-containing liposome, which is in a water-in-oil type (O / W type, oil-in-water type) nanosized in a range of 50 to 200 nm.

The present invention also provides a method for producing the aforementioned cosmetic composition.

(1) dissolving ceramide, cholesterol, fatty acid, phytosphingosine, and phospholipid in an oil to prepare a liquid crystal lipid part; (2) preparing liquid crystal forming composition (A) by adding the prepared liquid crystal lipid portion to a water soluble substance portion containing polyhydric alcohol and water; (3) dissolving a phospholipid in a polyhydric alcohol and mixing it with water to prepare a water phase; (4) A solution in which the ceramide is dissolved in alcohol is added to the aqueous solution prepared in the step (3) and mixed, and the mixed solution is formed into liposome using a high-pressure emulsifier to form a liposome composition ); (5) mixing the liquid crystal forming composition (A) prepared in the step (2) and the liposome composition (B) containing the sugar ceramide produced in the step (4).

Here, in the step (5), the content ratio of the liquid crystal forming composition (A) and the liposome composition (B) containing the saccharide ceramide may range from 70 to 95: 5 to 30% by weight, : 5 to 20% by weight, and more preferably 80 to 90: 10 to 20% by weight.

According to the present invention, it is possible to make a liquid crystal membrane having the same composition as that of a skin component by using ceramide / cholesterol / fatty acid / phytosphingosine / phospholipid, which is a constituent lipid component between skin cells, , Which is closely related to the intracellular matrix, is converted into nanoparticles by using a high-pressure homogenizer, thereby facilitating penetration of interstitial cells of skin cells and promoting the synthesis of ceramide in the dermal layer of skin, The robustness can be enhanced. In addition, since the liquid crystal film thus produced exhibits a high moisturizing effect due to a long-lasting effect, there is a distinctive advantage that the skin is healthier and more enriched.

Therefore, the sugar-ceramide-containing skin-like lipid liquid crystalline cosmetic composition prepared according to the method of the present invention can be applied to cosmetics to improve moisturizing ability, improve dry skin and improve atopy.

1 is a polarized microscope photograph of a cream formulation containing the cosmetic composition of Example 1. Fig.
2 is a polarized microscope photograph of a cream formulation containing the cosmetic composition of Example 2. Fig.
3 is a polarized microscope photograph of a cream formulation containing the cosmetic composition of Example 3. Fig.
4 is a polarized microscope photograph of a cream formulation containing the cosmetic composition of Example 4. Fig.
5 is a polarized microscope photograph of a cream formulation containing the cosmetic composition of Example 5. Fig.
FIG. 6 is a graph showing the skin moisture content evaluation results using the cream formulations prepared in the untreated group, Example 1 and Comparative Examples 4 to 5, respectively.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition containing Ceramide, Glucosylceramide, and Sphingolipid so as to have a structure and composition similar to those of the skin horny layer while protecting the skin from external substances and reducing water loss. A cosmetic composition for skin protection, and a process for producing the same.

To this end, in the present invention, after a constituent component of skin lipid existing in the skin epidermis is made into a cream composition of liquid crystal type, a sugarcerefmide present in the dermis is further added by nanoliposomes The skin permeation can be facilitated and the sugar ceramide can reach the dermal layer, and the skin moisturizing power can be maximized.

<Liquid Crystal Cosmetic Composition Containing Glyceride-Containing Skin-Like Lipid Film>

The cosmetic composition according to the present invention comprises a liquid crystal forming composition (A) and a saccharide ceramide-containing liposome composition (B), characterized in that they are controlled to a specific content range.

In the cosmetic composition of the present invention, the liquid crystal forming composition (A) comprises (i) a liquid crystal lipid part and (ii) a water-soluble substance part.

In the liquid crystal forming composition (A) of the present invention, the liquid crystal lipid (i) may include ceramides, cholesterol, fatty acids, phytosphingosine and phospholipids.

The ceramide, cholesterol, fatty acid, and phytosphingosine are usually the main constituents of intercellular quality. In addition, phospholipid is the main material that constitutes the cell wall, and it is possible to form liquid crystal, and it has structure and composition similar to cell interstitial quality, thereby effectively preventing moisture evaporation of skin epidermis and maximizing moisture retention in skin.

The ceramide and cholesterol used in the present invention can be used without limitation in the conventional materials known in the art.

The fatty acid may be any conventional component known in the art. For example, a straight chain saturated fatty acid having 12 to 30 carbon atoms, preferably 18 to 26 carbon atoms may be used. Examples of the fatty acid include stearic acid, palmitic acid, oleic acid, linoleic acid, linoleic acid, arachidic acid, behenic acid, lignoceric acid, cetric acid, montanic acid and melissic acid. Two or more species can be used.

Phytosphingosine is an intermediate in the biosynthetic pathway of ceramides. It is applied to the skin to supplement deficient ceramide components or to allow skin cells to directly synthesize ceramides. Such a phytosphingosine may be a conventional one known in the art. For example, the phytosphingosine may be isolated from yeast, and its structure and properties are the same as those of phytosphingosine present in human skin.

Phospholipid (lecithin) is the main constituent of the cell membrane as a cell constituent. Phospholipids play an important role in the regulation of enzymatic reaction by providing various enzymes' working environment, and they act as a secondary transporter in cells by various stimuli. As the phospholipid, there may be used any of the conventional components known in the art without limitation, including, but not limited to, hydrogenated lecithin, hydrogenated phosphatidylcholine, phospholipid, hydrogenated lysophosphatidylcholine, Lecithin, hydrooxylated lecithin, unsaturated lecithin, or a mixture of one or more thereof.

In the liquid crystal forming composition (A) of the present invention, the water-soluble substance portion (ii) includes water, a polyhydric alcohol or a mixture thereof.

The polyhydric alcohol may be any conventional polyhydric alcohol known in the art without limitation, and examples thereof include glycerin, diglycerin, dipropylene glycol, propylene glycol, 1,3-butylene glycol, 1,2-butyl 2,3-butylene glycol, hexylene glycol, polyethylene glycol, trimethylene glycol, and mixtures of at least one of these.

(I) a liquid crystal lipid part and (ii) a water-soluble substance part, wherein the liquid crystal lipid part (i) contains 0.15 to 4.0% by weight of ceramide, 0.05 to 2.0 wt% of cholesterol, 0.05 to 3.0 wt% of fatty acid, 0.05 to 2.0 wt% of phytosphingosine, 0.5 to 10.0 wt% of phospholipid and 1.0 to 20.0 wt% of oil, wherein the water soluble substance portion (ii) May be a composition comprising water in a residual amount satisfying 3.0 to 20% by weight of the polyhydric alcohol and 100% by weight of the total. In a preferable example thereof, the liquid crystal lipid part (i) comprises 1.0 to 4.0% by weight of ceramide, 0.3 to 2.0% by weight of phytosphingoglycein, 1.0 to 3.0% by weight of fatty acid, 0.5 to 2.0% by weight of cholesterol, 2.0 to 10.0% 2.0 to 7.0% by weight, and the water-soluble substance portion (ii) may be a composition comprising 4.0 to 15.0% by weight of a polyhydric alcohol and a balance of water satisfying 100% by weight as a whole.

In the cosmetic composition according to the present invention, the saccharide-containing liposome composition (B) may be composed of a saccharide ceramide, a phospholipid, a polyhydric alcohol and water.

The saccharide may be any conventional material known to those skilled in the art without any particular limitation, provided that the saccharide has a structure in which the saccharide is bound to the ceramide. Non-limiting examples thereof include glucosylceramide, galactosylceramide, glucocerebroside, galactocerebroside, lactosylceramide, or a mixture of one or more thereof.

In the present invention, a saccharide is generally referred to as a ceramide having a backbone of ceramide and a glucose group or the like as a substituent. The substituent may include, without limitation, monosaccharides such as glucose, galactose, sucrose, and polysaccharides. The molecular weight of the sugarcerefamide is not particularly limited, and may range from 700 Da to 780 Da, for example.

The saccharide used in the present invention is preferably a substance belonging to ceramides having a molecular weight of 746.11 Da and having a glucose group represented by the following formula (1). The saccharide material of Formula 1 was prepared by chemical synthesis using sugar and ceramide as a starting material, and was supplied by KIST (Korea Institute of Science and Technology).

In the sugarcerefacial-containing liposome composition (B) of the present invention, the phospholipid may be a conventional material known in the art. For example, the phospholipid may be the same as or different from the phospholipid component used in the liquid crystal lipid portion of the liquid crystal forming composition (A) can do.

The polyhydric alcohol may also be the same as or different from the component used in the water-soluble substance portion of the liquid crystal forming composition (A).

The saccharide-containing liposome composition (B) according to the present invention comprises 0.015 to 2.0% by weight of sugar ceramide, 1.0 to 15.0% by weight of phospholipid, 1.0 to 50.0% by weight of alcohol, And a balance of water satisfying 100 wt% of the composition. Preferable examples thereof include a composition comprising 10.0 to 25.0% by weight of polyhydric alcohol, 1.0 to 5.0% by weight of phospholipids, 0.1 to 2.0% by weight of sugar ceramide, 5.0 to 15.0% by weight of alcohol and 100% .

The cosmetic composition of the present invention is formed by mixing the liquid crystal forming composition (A) and the saccharide ceramide-containing liposome composition (B), wherein the content ratio of the liquid crystal forming composition (A) May be in the range of 70 to 95: 5 to 30% by weight, preferably 80: 95: 5 to 20% by weight, more preferably 80: 90: 10 to 20% by weight.

The cosmetic composition has a structure and composition similar to the stratum corneum and intercellular quality of human skin, and when applied to a formulation, it forms a liquid crystal to enhance skin absorption and improve skin penetration ability.

In general, liquid crystals are widely used in cosmetics, and they are melted in an oil phase and then formed at the interface by the diffusion of oil, thereby contributing to enhancement of skin moisturizing power. The first reason that the liquid crystal contributes to improving the moisturizing power is due to the structural similarity between liquid crystal and skin lipid. Due to this structural similarity, the liquid crystal component is spread well on the surface of the skin as compared to other components, resulting in smooth and smooth skin. Another reason is that liquid crystals collect a certain amount of bounded water, which is unlikely to evaporate unlike free water. That is, the collected crystals stay like liquid crystals on the skin, and consequently contribute to increase the moisture content of the skin. The cosmetic composition of the present invention forms a liquid crystal when applied as a final formulation. The liquid crystal to be formed at this time may be a liquid crystal of Maltecross. Here, the liquid crystal may be a single layer or a multilayer, and may be preferably a multilayer.

On the other hand, emulsions containing a Lamellar liquid crystal structure have advantages such as high stability, control of drug release, easiness of production, excellent feeling of use, and maintenance of moisturizing effect. Lamellar is a lamellar structure composed of phospholipids, oily membranes, and water membranes. It is very similar to the intercellular structure of the skin stratum corneum, and this structure prevents moisture evaporation from the skin. In order to form liquid crystals having the above-described structure, it is preferable to use polyoxyethylene stearate, polyoxyethylene dihydroxy stearate, microcrystalline wax, higher alcohol (having 12 to 18 carbon atoms), higher fatty acid (having 12 to 18 carbon atoms) (Carbon number 12 to 16). When a general multi-layer liquid crystal is formed by a combination of phospholipid and surfactant, a phenomenon of precipitation of ceramide occurs, and the precipitated ceramide is involved in liquid crystal formation, The structure collapsed and the observation of the liquid crystal structure was unclear. In contrast, in the present invention, precipitation of ceramides can be prevented through the formation of the liquid crystal of the liquid crystal, and the structure of the liquid crystal can be clearly confirmed.

In addition, the cosmetic composition of the present invention may include an o / w type oil-in-water type nano emulsion particle. Preferably, the glyc ceramide-containing liposome is a nanoparticulate o / w type ) Emulsion. At this time, the average particle size of the nanoparticles may be in the range of 50 to 200 nm, and improvement in skin penetration and formulation stability may be improved if the above range is satisfied.

The term "nanoparticulate saccharide-containing liposome" in the present invention is a colloidal particle in which particles composed of an amphipathic molecule having a water-soluble hair and an insoluble tail are mutually spontaneously bonded and aligned, and have components and structures similar to the cell membrane. Accordingly, the cosmetic composition containing the same can increase the skin protection effect by restoring damaged skin barrier, strengthening skin barrier, and alleviating skin irritation.

As described above, the cosmetic composition of the present invention has a composition similar to the intercellular lipid of the skin barrier, thus forming a skin-like lipid in the application of the formulation. Therefore, the cosmetic composition can be used for skin protection or skin moisturizing.

Meanwhile, the cosmetic composition of the present invention includes components commonly used in cosmetic compositions, in addition to containing the above-mentioned ingredients as an effective ingredient. Such as conventional antioxidants, stabilizers, solubilizers, vitamins, pH adjusting agents, colorants, flavoring agents, pigments and fragrances, which are known in the art, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, the present invention relates to a cosmetic composition which has a formulation of a flexible lotion (skin), a convergent lotion, a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurate, sarcosinates, fatty acid amides Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

&Lt; Preparation method of cosmetic composition >

Hereinafter, a method for producing a cosmetic composition according to the present invention will be described. However, the present invention is not limited to the following production methods, and the steps of each process may be modified or optionally mixed as required.

Here, the content of each constituent component is% by weight, and can be expressed in terms of weight% or weight part in combination.

The method for producing a cosmetic composition according to the present invention can be produced by preparing a liquid crystal forming composition (A) comprising a water-soluble substance part and a liquid crystal lipid part and a glycosamide-containing liposome composition (B) .

(1) dissolving ceramide, cholesterol, fatty acid, phytosphingosine, and phospholipid in an oil to prepare a liquid crystal lipid part; (2) preparing liquid crystal forming composition (A) by adding the prepared liquid crystal lipid portion to a water soluble substance portion containing polyhydric alcohol and water; (3) dissolving a phospholipid in a polyhydric alcohol and mixing it with water to prepare a water phase; (4) A solution in which the ceramide is dissolved in alcohol is added to the aqueous solution prepared in the step (3) and mixed, and the mixed solution is formed into liposome using a high-pressure emulsifier to form a liposome composition ); (5) mixing the liquid crystal forming composition (A) prepared in the step (2) and the liposome composition (B) containing the sugar ceramide produced in the step (4).

Hereinafter, the manufacturing method will be described separately for each step.

(A) First, a liquid crystal forming composition comprising a component constituting a skin-like lipid membrane is prepared.

In the present invention, the liquid crystal forming composition (A) comprises (i) a liquid crystal lipid part comprising constituents of a skin-like lipid membrane; And (ii) a water-soluble substance portion corresponding to the medium.

(1) a step of dissolving ceramide, cholesterol, fatty acid, phytosphingosine and phospholipid in an oil to prepare a liquid crystal lipid part, and (2) a step of dissolving a polyhydric alcohol and water (Liquid crystal forming part) to the water-soluble substance part including the water-soluble substance and mixing the liquid crystal lipid part (liquid crystal forming part) to prepare the liquid crystal.

In the step (A), it is preferable that the liquid crystal lipid part (liquid crystal forming material part) including ceramides, cholesterol, fatty acid, phytosphingosine and phospholipids is added to oil and dissolved by heating at 80 to 85 ° C.

The oil component may be a conventional component known in the art. Examples of the oil component include a silicone oil, a hydrocarbon oil, a higher fatty acid oil, an ester oil, a glyceride oil, a lanolin oil, a vegetable oil, .

The oil-in-water (O / W) type emulsion is prepared by adding the liquid crystal lipid part (liquid crystal forming material part) dissolved in the above-described manner to the water-soluble substance part, thereby forming the liquid crystal part of the Maltose type.

At this time, the water-soluble substance portion preferably has a temperature of 75 to 80 ° C. The water-soluble substance portion may be a polyhydric alcohol, water, or a mixture thereof, preferably a mixture of a polyhydric alcohol and water.

According to the present invention, the liquid crystal forming composition (A) comprising the constituents of the skin lipids comprises (a) from 0.15 to 4.0% by weight of ceramides, (b) from 0.05 to 2.0% by weight of cholesterol, (d) from 0.05 to 2.0% by weight of phytosphingosine, (e) from 0.5 to 10.0% by weight of phospholipids, (g) from 1.0 to 20.0% by weight of oil and (h) 3.0 to 20.0% by weight of polyhydric alcohol; And a water-soluble substance (water-containing substance) containing a residual amount of water.

(B) a ceramide-containing liposome composition is prepared.

(3) dissolving a phospholipid in a polyhydric alcohol and mixing it with water to prepare a water-soluble part; And (4) dissolving the perithecide in the alcohol, adding the solution to the aqueous phase prepared in the step (3), mixing the mixture, and forming the liposome using the high-pressure emulsifier in the mixed solution .

In the step (B), first, a phospholipid is dissolved in a polyhydric alcohol and water (water) is added to prepare a water-soluble part. In this case, it is preferable that the polyhydric alcohol is heated to 75 to 80 占 폚.

Thereafter, the sugarcerefamide is dissolved in alcohol (ethanol), and a solution in which the sugarcereamide is dissolved is slowly added to the above-mentioned water while being emulsified to prepare an O / W (oil in water) emulsion.

At this time, the alcohol may be a lower alcohol having 1 to 6 carbon atoms capable of dissolving the saccharide. It is preferably ethanol.

Thereafter, an oil-in-water (O / W) emulsion is passed through a high-pressure emulsifier to produce a liposome. At this time, the high-pressure emulsifier can be used more than once. Preferably 600 bar (8000-9000 psi), and then passed through two 1000 bar (14000-15000 psi) to produce the liposome.

The saccharide-containing liposome composition (B) according to the present invention comprises (i) 0.015 to 2.0% by weight of a peroxide ceramide, (j) 1.0 to 15.0% by weight of a phospholipid, (k) 1.0 to 20.0% 50.0% by weight.

(5) The liquid crystal forming composition (A) and the liposome composition (B) containing sugar ceramide are mixed to finally prepare a composition for forming a skin lipid membrane liquid crystal containing sugar ceramide (step C).

In the above step (C), the composition A, which is the liquid crystal portion of the liquid crystal material, and the composition B, which is a saccharide ceramide liposome, are emulsified by using a homomixer. May be in the range of 70 to 95: 5 to 30% by weight, preferably 80 to 95: 5 to 20% by weight, more preferably 80 to 90: 10 to 20% by weight, Lt; / RTI &gt;

The cosmetic composition of the present invention prepared as described above contains ceramide, fatty acid (cholesterol), and phytosphingosine as main components in order to maintain the same composition as the skin cell membrane, thereby enhancing the skin lipid membrane and improving the moisturizing power. In addition, it contains an additional saccharide in the dermis to facilitate penetration of the skin, resulting in the synthesis and fractionation of the ceramide in the skin. Therefore, it is possible to maximize skin permeability and moisturizing power, and to exhibit excellent effects in improving dryness of skin, improvement of atopy, and improvement of moisturizing power (see Tables 3 to 4 and Figs. 1 to 6).

Hereinafter, examples and comparative examples of the present invention will be described in more detail. It will be apparent to those skilled in the art that these embodiments are provided by way of illustration only for the purpose of more particularly illustrating the present invention and that the scope of the present invention is not limited by these embodiments .

[Example 1]

1-1. The liquid crystal forming composition (A)

The compositions of Step A were prepared using the ingredients listed in Table 1 below.

More specifically, the oil was heated to 80 to 85 占 폚, and then the ceramide, phytosphingosine, fatty acid, cholesterol, and phospholipid were added with stirring to prepare a liquid crystal lipid. Also, the water-soluble substance part was mixed with polyhydric alcohol and water, heated to 80 to 85 ° C and stirred. The liquid crystal lipid part was added to the warmed water-soluble substance part and emulsified using a homogenizer to prepare an oil-in-water (O / W) type emulsion to form a liquid crystal part of a Maltose type.

1-2. Sugar ceramide  contain Liposome  The composition (B)

20% by weight of 1,3-butylene glycol, and 3% by weight of lecithin were mixed to prepare a liposome composition containing the saccharide-containing ceramide, heated at 75 to 80 DEG C and dissolved, By weight of water and 65% by weight of water. 2% by weight of sugar ceramide was added to 10% by weight of ethanol and completely dissolved. Then, the solution was slowly added to water to be primary emulsified. The primary emulsified liquid was subjected to high pressure emulsification once at 600 bar through a high pressure emulsifier and then subjected to high pressure emulsification twice at 1000 bar to prepare a liposome composition containing sugar ceramide.

1-3. Cosmetics  (C)

The composition of step A, which is the above-mentioned latex liquid crystal part, and the composition of step B, which is a sugar-ceramide liposome, were mixed at a composition ratio of A composition: B composition = 90 wt%: 10 wt%, using a homomixer, Minute to prepare a liquid crystal cosmetic composition having a skin-like membrane containing the final saccharide ceramide.

[Examples 2 to 5]

The liquid crystal cosmetic compositions of Examples 2 to 5 were prepared in the same manner as in Example 1, except that the composition shown in Table 1 was used.

[Comparative Examples 1 to 3]

The liquid crystal cosmetic compositions of Comparative Examples 1 to 3 were prepared in the same manner as in Example 1, except that the composition shown in Table 1 was used.

[Experimental Example 1. Stability Evaluation of Liquid Crystal Composition by Temperature]

The stability of the ceramide-containing skin-like lipid membrane-containing liquid crystal phase compositions of Examples 1 to 5 and Comparative Examples 1 to 3 prepared through the above three steps was tested for each temperature.

The test period was 12 weeks. Experiments were carried out at 4 ° C, 25 ° C, 45 ° C and 60 ° C in order to investigate the conditions of temperature retention.

As a result of the test, the compositions of Examples 1 to 5 did not cause any problems during the 12-week period, but in Comparative Example 1, the sides of the formulation immediately after preparation were not smooth as compared with Examples 1 to 5. This phenomenon is thought to be due to the increase of amount of powder material such as ceramide, phytosphingosine, etc., and it is considered that the surface of the formulation is not smooth smoothly. Although there is no problem in temperature stability for 12 weeks, .

In the case of Comparative Example 2, the experiment was carried out by increasing the content of the phospholipid, which is the main component of the liquid crystal formation, which acts as an emulsifier, and the amount of oil which acts as a solvent. The surface state of the formulation immediately after the experiment was similar to that of Comparative Example 1, but a fine phase separation phenomenon was observed at the storage stability at 60 ° C for 4 weeks. Phase separation phenomenon could be observed even at storage stability of 45 ℃ at 10 weeks.

Also, in Comparative Example 3, although the content of oil and phospholipids in the liquid crystal lipid portion was increased, due to excessive content of the liquid crystal lipid portion, too high viscosity was formed in the production step, which made it difficult to prepare the formulations. The phenomenon can be confirmed. This is presumed to be due to the fact that the emulsion is not properly formed due to the content of the liquid crystal lipid portion and that the liquid crystal formation is not properly formed.

&Lt; Evaluation of physical properties of cream formulations to which liquid crystal phase composition is applied &

In order to confirm the effects on the moisture evaporation of the skin, the improvement of the skin dryness and the liquid crystal forming ability of the liquid crystal composition prepared in the above step 3, a composition for forming a skin lipid membrane liquid crystal was mixed with a common cream composition. The composition of the cream applied on the basis of the above composition is shown in Table 2 below.

cream

Furtherance division ingredient Example 1 Floating DC # 345 4.0 DC 200/100 CS 0.5 Waglinol MCT 4.0 Arlacel # 165 0.8 Tween # 60 1.1 Lanette O 1.5 Squalane 3.0 GMS 105 1.2 CEH 3.0 Water top DI-Water 59.21 Glycerine 4.0 1,3BG 5.0 Hyaluronic acid 1.0 EDTA-2Na 0.02 CP 940 0.16 Skin lipid liquid crystal formation
Composition Skin Lipid Complex 5.0 antiseptic Phenoxy ethanol 0.5 corrector TEA 0.14

[ Experimental Example  2. Evaluation of stability of cream formulation]

In order to confirm the applicability of the cream to the liquid crystal compositions of Examples 1 to 5 in which stability was secured by storage temperature, formulation stability was confirmed at 4 ° C, 25 ° C and 45 ° C for 24 weeks and at 60 ° C for 12 weeks The experiment was carried out.

As a result of the experiment, it was found that the cream formulations of Examples 1 to 5 did not exhibit phase separation and crystal precipitation for 4 weeks, and thus had formulation stability. This is because, when the contents of ceramide and phytosphingosine are within a certain range in determining the composition of the liquid crystal lipid part, the formulation stability is easy to manufacture, whereas when the content of these components is higher than a certain level, there is a problem in formulation stability and manufacturing I was able to confirm the fact.

[ Experimental Example  3. Evaluation of Skin Moisture Evaporation Rate of Cream Formulation]

The effect of the cream applied with the liquid crystal composition prepared in Examples 1 to 5 on the skin moisture evaporation amount was measured.

Tape stripping was performed on the upper arm of the arm in a constant temperature and humidity chamber at 22 DEG C and 45% relative humidity to weaken the skin barrier. Creams applied in Examples 1 to 5 were applied to 20 The amount of moisture evaporation was measured after a certain amount (0.03 g / 16 cm 2) of the composition was applied to the subject and rubbed well. The TEWL was measured with a Tewameter TM 210, a water vapor evaporator from C-K (Courage + Khazaka, Cologne, Germany). The evaporation amount of moisture was measured five times at intervals of 1 minute every 12 hours after application, and an average value was obtained. The results are shown in Table 3 below.

As a result, it was confirmed that the moisture retention effect in the skin was relatively decreased when the content of the lipid component was too small (see Table 3).

division
(Moisture evaporation amount) Example 1 (%) Example 2 (%) Example 3 (%) Example 4 (%) Example 5 (%) After 0 hours 0 0 0 0 0 After 12 hours 8 14 17 17 14 After 24 hours 13 21 25 26 25 After 36 hours 17 33 34 35 31 After 48 hours 21 39 40 41 40

[ Experimental Example  4. Evaluation of sensory test]

In order to evaluate the skin dryness improving effect (reduction in pulling and roughness, increase in glossiness) and skin irritation of the cosmetic composition for skin moisturizing according to the present invention, the cream applied with the liquid crystal composition prepared in Examples 1 to 5 was subjected to a sensory test Respectively.

More specifically, the above-mentioned creams were continuously used for 10 women 20 years old or older twice a day for 2 weeks. Skin dryness improvement and skin irritation sensory evaluation were evaluated for skin pulling and roughness reduction, itching, stinging and erythema. The evaluation was very good (5 points), excellent (4 points), normal (3 points) Poor (2 points), and very poor (1 point). These results are shown in Table 4 below.

As a result of the test, the score of the cosmetic composition of Example 1 was 4.8 points and was evaluated very favorably. In particular, it was confirmed that, compared with Examples 2 to 5, the skin roughness reduction and the skin irritation degree show an effective effect.

score Example 1 Example 2 Example 3 Example 4 Example 5 Subject 1 5 3 2 4 One Subject 2 4 5 3 2 One Subject 3 5 4 2 One 3 Subject 4 5 3 2 One 4 Subject 5 5 4 3 2 One Subject 6 4 5 2 3 One Subject 7 5 3 4 One 2 Subject 8 5 4 3 2 One Subject 9 5 3 4 2 One Subject 10 5 3 2 4 One Average 4.8 3.7 2.7 2.2 1.6

[ Experimental Example  5. Evaluation of liquid crystal forming ability]

When a composition prepared in Examples 1 to 5 was applied to a cream formulation, formation of a multi-lamellar barrier to confirm whether or not the liquid crystal forming ability was ultimately retained was confirmed through a polarizing microscope observation.

As a result of the experiment, it was confirmed that the composition prepared in Example 1 showed a multi-lamellar type of the liquid crystal phase, and good results in terms of uniformity of the liquid crystal phase were obtained. A micrograph of the liquid crystal thus observed is shown in Fig.

On the other hand, in the case of the compositions prepared according to Examples 2 to 5, the density and uniformity of the liquid crystal were reduced when applied to a cream formulation, and it was confirmed that the liquid crystals were partially observed (see FIGS. 2 to 5).

[ Experimental Example  6. Skin moisture evaluation]

The changes in skin hydration according to the presence or absence of ceramide were examined as follows.

As a sample, a general cream composition containing no step A and step B was prepared and used as comparative example 4, and a composition not containing the glycosamide-containing liposome composition of step B in the same composition as in Example 1 was used as Comparative Example 5 , And a composition containing the saccharide ceramides, including both the compositions of steps A and B, was prepared as in Example 1. These compositions are shown in Table 5 below.

To measure the skin moisture content, 20 creams were applied to the subjects in Example 1 and the creams prepared in Comparative Examples 4 to 5 twice a day, and 3 times, 5 days, 8 days, And the change of the moisture content was measured. Moisture content was measured in a constant temperature and humidity chamber at 22 ° C and a relative humidity of 45% using a skin moisture meter (Corneometer, CM825, Courage-Khazaka Electronic GmbH, Germany) of C-K (Courage + Khazaka, Cologne, Germany). The results are shown in FIG.

cream
Furtherance division ingredient Example 1 Comparative Example 4 Comparative Example 5 Floating DC # 345 4.0 4.0 4.0 DC 200/100 CS 0.5 0.5 0.5 Waglinol MCT 4.0 4.0 4.0 Arlacel # 165 0.8 0.8 0.8 Tween # 60 1.1 1.1 1.1 Lanette O 1.5 1.5 1.5 Squalane 3.0 3.0 3.0 GMS 105 1.2 1.2 1.2 CEH 3.0 3.0 3.0 Water top DI-Water 59.21 64.21 59.21 Glycerine 4.0 4.0 4.0 1,3BG 5.0 5.0 5.0 Hyaluronic acid 1.0 1.0 1.0 EDTA-2Na 0.02 0.02 0.02 CP 940 0.16 0.16 0.16 Skin lipid liquid crystal formation
Composition Skin Lipid Complex 5.0 - - Without sugar ceramide
Composition Skin Lipid Complex
(Without sugar ceramide liposome) - - 5.0 antiseptic Phenoxy ethanol 0.5 0.5 0.5 corrector TEA 0.14 0.14 0.14

As a result of the experiment, the creams of Comparative Example 4 and Comparative Example 5 showed a significant change in skin moisture content from the 8th day of the untreated group.

In contrast, the cream of Example 1 containing both the skin lipid membrane composition and the saccharide ceramide liposome composition exhibited significant skin moisture retention change from day 3, and when the final day difference was reached, it was significantly higher than that of Comparative Examples 4 to 5 Was measured (see Fig. 6). These results show that the inclusion of sugar ceramides has a large impact on skin moisture retention.

Claims (12)

(A) a liquid crystal forming composition comprising a water-soluble substance portion and a liquid crystal lipid portion; And
(B) a ceramide-containing liposome composition
Wherein the liposome composition containing the saccharide complex is contained in an amount ranging from 5.0 to 30.0% by weight based on the total weight of the cosmetic composition, wherein the liposome composition comprises a saccharide ceramide-containing skin-like lipid composition. 2. The composition of claim 1, wherein the saccharide-containing liposome composition (B) comprises 0.015 to 2.0% by weight of sugar ceramide, 1.0 to 15.0% by weight of phospholipids, 1.0 to 50.0% by weight of polyhydric alcohol, And a remaining amount of water that satisfies 100% by weight of the composition. The saccharide-containing skin according to claim 2, wherein the saccharide ceramide is at least one selected from the group consisting of glucosylceramide, galactosylceramide, glucocerebroside, galactocerebroside and lactosylceramide. A liquid crystal cosmetic composition having a similar lipid composition. 3. The composition of claim 2, wherein the phospholipid is selected from the group consisting of hydrogenated lecithin, hydrogenated phosphatidylcholine, phospholipid, hydrogenated lysophosphatidylcholine, hydrogenated lyso lecithin, hydrooxylated lecithin and unsaturated lecithin. Wherein the lipid is at least one selected from the group consisting of liposomes and liposomes. The liquid crystal composition according to claim 1, wherein the liquid crystal forming composition (A) comprises a liquid crystal lipid part and a water soluble substance part,
Wherein the liquid crystal lipid part comprises 0.1 to 4.0% by weight of ceramides, 0.05 to 2.0% by weight of cholesterol, 0.05 to 3.0% by weight of fatty acids, 0.05 to 2.0% by weight of phytosphingosine, 0.5 to 10.0% by weight of phospholipids and 1.0 to 20.0% Including,
Wherein the water-soluble substance portion is a composition comprising 3.0 to 20% by weight of a polyhydric alcohol and a remaining amount of water satisfying 100% by weight of the whole. The liquid crystal cosmetic composition according to claim 1, which has a skin-like lipid composition containing saccharide ceramides, which forms a liquid crystal. The cosmetic composition according to claim 1, wherein the cosmetic composition is an oil-in-water (O / W) type emulsion in which the saccharide-containing liposome is nanoparticulate in a range of 50 to 200 nm. . The liquid crystal cosmetic composition according to claim 1, which is used for skin protection or skin moisturizing enhancement, having a sugar-ceramide-containing skin-like lipid composition. (1) dissolving ceramide, cholesterol, fatty acid, phytosphingosine, and phospholipid in an oil to prepare a liquid crystal lipid part;
(2) preparing liquid crystal forming composition (A) by adding the prepared liquid crystal lipid portion to a water soluble substance portion containing polyhydric alcohol and water;
(3) dissolving a phospholipid in a polyhydric alcohol and mixing it with water to prepare a water phase;
(4) A solution in which the ceramide is dissolved in alcohol is added to the aqueous solution prepared in the step (3) and mixed, and the mixed solution is formed into liposome using a high-pressure emulsifier to form a liposome composition );
(5) mixing the liquid crystal forming composition (A) prepared in the step (2) and the liposome composition (B) containing the sugar ceramide produced in the step (4)
Like lipid-forming liquid crystal cosmetic composition containing a saccharide. The liquid crystal forming composition according to claim 9, wherein the liquid crystal forming composition (A) of the step (2) comprises a liquid crystal lipid part and a water soluble substance part,
Wherein the liquid crystal lipid part comprises 0.1 to 4.0% by weight of ceramides, 0.05 to 2.0% by weight of cholesterol, 0.05 to 3.0% by weight of fatty acids, 0.05 to 2.0% by weight of phytosphingosine, 0.5 to 10.0% by weight of phospholipids and 1.0 to 20.0% , &Lt; / RTI &gt;
Wherein the water-soluble substance portion has a composition comprising 3.0 to 20% by weight of a polyhydric alcohol and a remaining amount of water that satisfies 100% by weight of the total weight of the sugar-ceramide-containing skin-like lipid composition type liquid crystal cosmetic composition. The method of claim 9, wherein the liposome composition (B) containing the saccharide in step (4) comprises 0.015 to 2.0% by weight of sugar ceramide, 1.0 to 15.0% by weight of phospholipid, 1.0 to 50.0% And a remaining amount of water satisfying 100% by weight of the total weight of the skin-like lipid composition-containing liquid crystal composition. The method according to claim 9, wherein the content ratio of the liquid crystal forming composition (A) to the liposome composition (B) containing the sugar ceramide is in the range of 70 to 95: 5 to 30% by weight in the step (5) Like skin-like lipid-forming type liquid-crystalline cosmetic composition.

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