KR20150057014A - Cosmetic composition containing extracts of Curcuma longa Linne for controlling anti-acne or anti-comedone - Google Patents

Abstract

The present invention relates to a cosmetic composition which is used to alleviate acne and includes a turmeric leaf (Curcuma longa) extract. The turmeric leaf extract has an excellent antibacterial activity against the bacteria causing the acne and an anti-inflammatory activity to alleviate or prevent the acne. Accordingly, the composition can also be used to develop a drug for treating the acne.

Description

[0002] Cosmetic composition containing extracts of Curcuma longa Linne for controlling anti-acne or anti-comedone,

The present invention relates to a cosmetic composition for improving acne containing an extract of turmeric leaves as an active ingredient.

Acne is a skin disorder caused by abnormal sebaceous glands around the hair follicle. In normal condition, sebaceous glands rise up along the hair follicle wall and are released through the skin. In acne patients, however, sebaceous glands are not released to the outside and are trapped around the hair follicles. These acne are chronic inflammatory diseases of the epidermis that occur mainly on the face, chest, and neck of puberty men and women, and form comedones, papules, pustules, cysts, or nodules. However, some people continue to have acne until the late 20s or 30s, and sometimes later, and some people develop acne for the first time since becoming an adult. Regardless of gender, they occur in almost the same proportion, but men have more severe and longer-lasting acne than women. In the case of female acne, acne may become worse or better depending on hormone changes or misuse of cosmetics depending on the menstrual cycle. In particular, acne due to use of cosmetics often persists until it becomes an adult.

Treatment of these acne is medication, which can be roughly divided into topical application and oral therapy, and mild acne can be treated with topical application alone. However, if symptoms are severe, topical application and oral therapy are effective. Representative drugs used as topical application agents include benzoyl peroxide, azelaic acid, retinoic acid, salicylic aicd, sulfur, topically applied antibiotics These preparations do not exhibit a sufficient antibacterial effect against acne bacterium only by a small amount, and there is a fear that side effects such as non-spoilage or dryness of skin may occur when overdosing.

On the other hand, Curcuma longa Linne is divided into Ulguk and Turmeric depending on the area where Ulgum is medicinal plant. According to the plant form and the weakness in the old consent document, Ulugum uses the roots of plants, while Turmeric uses stem roots of plants, and the taste is much higher than Ulugum, and the color is darker than Ulugum. In addition, small spiny fluffy flowers on the back side of the leaves grow pink in spring, tropical Asia is the origin, and turmeric plants do not grow naturally in Korea, and perennial herbaceous plants that grow naturally in tropical regions and southern regions of China to be. In herbal medicine, it is said that the use of turmeric as a herbal medicine is warmer, which promotes blood circulation and eliminates pain.

Curcumin, which is a major physiologically active ingredient in turmeric, has been reported to have antitumor, antioxidant and anti-amyloid activities, and it has been reported that it can be used for the development of new drugs with high added value or development of health foods It is alive.

However, there is no study on the effects of turmeric in the treatment of acne.

Korean Patent Publication No. 2011-0000082

In order to solve the above problems, it is an object of the present invention to provide a cosmetic composition for improving acne comprising an extract of Ganoderma lucidum as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for the prevention and treatment of acne containing an extract of Ganoderma lucidum as an active ingredient.

Accordingly, the present invention provides a cosmetic composition for improving acne comprising an extract of turmeric leaves as an active ingredient.

In one embodiment of the present invention, the composition may contain 0.001 to 20.0% by weight of an extract of a Korean squid leaf, based on the total weight of the composition.

In one embodiment of the present invention, the Ganoderma lucidum extract is selected from the group consisting of purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, glycerol, propylene glycol, 1,2-butylene glycol, methyl acetate, ethyl acetate, Ethyl ether, and dichloromethane. The extract may be extracted with a solvent selected from the group consisting of ethanol, ethanol, ethyl ether, and dichloromethane.

In addition, the present invention provides a pharmaceutical composition for preventing and treating acne containing an extract of Ganoderma lucidum as an active ingredient.

The present invention relates to a cosmetic composition for improving acne containing a Ganoderma lucidum extract. The Ganoderma Leaf Extract of the present invention is excellent in antibacterial activity against bacteria causing acne and has excellent anti-inflammatory activity and thus can be used in a cosmetic composition for improvement and prevention of acne and also useful for the production of pharmaceuticals for the treatment of acne There is an effect that can be used.

The present invention is characterized by providing a cosmetic composition for improving acne containing Curcuma longa Linne leaves extract as an active ingredient.

The present inventors have found out that the extract obtained from the leaves of turmeric is very effective in treating and improving acne while studying to extract active ingredients that can improve and treat acne more effectively than natural synthetic compounds.

That is, according to one embodiment of the present invention, the antimicrobial activity against acne causative bacteria of the extracts obtained from ethanol extract of turmeric leaves was more excellent than that of conventional antimicrobial agents .

In addition, the extract of Ganoderma lucidum of the present invention has an excellent anti-inflammatory activity.

The acne and acne rashes caused by acne bacteria or environmental factors belong to inflammatory skin diseases. Inflammatory skin disease refers to a disease that causes a series of clinical signs and symptoms such as itching, edema, erythema or exfoliation due to various stimulating factors causing a series of inflammatory reactions in the skin epithelium. In addition to acne and acne rash, inflammatory skin diseases include atopic dermatitis and seborrhoic dermatitis.

Therefore, the extract of Ganoderma lucidum of the present invention can inhibit the inflammatory reaction and thus has an effect of effectively preventing, ameliorating and treating inflammatory skin diseases, particularly acne, which can be caused by inflammation.

In addition, the extract of Ganoderma lucidum of the present invention has antimicrobial activity against acne bacterium, and preferably has antibacterial activity against Propionibacterium acnes.

Therefore, the present invention can provide a cosmetic composition for improving acne comprising an extract of Ganoderma lucidum as an active ingredient.

The Ganoderma Leaf Extract can be obtained by extracting and isolating from nature using an extraction and separation method known in the art, and the 'extract' defined in the present invention can be extracted from a leaf of turmeric using an appropriate solvent For example, crude extract of turmeric, polar solvent-soluble extract or non-polar solvent-soluble extract. Preferably, the extract may be an extract derived from turmeric leaves.

As an appropriate solvent for extracting the extract from the turmeric leaves, any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, and for example, purified water, methanol alcohol having 1 to 4 carbon atoms, acetone, ether, benzene, chloroform (including methanol, ethanol, propanol, isopropanol, and butanol) various solvents such as chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. It is preferable to use ethanol (alcohol), more preferably 70% ethanol.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the extract of Chenghuang leaf of the present invention, and any known method can be used.

In addition, in the present invention, the extract of Chenghuang leaf is a concept including all extracts, fractions and purified products obtained in each step of extraction, fractionation or purification, and diluted solution, concentrate or dried product thereof.

When the composition of the present invention is manufactured from a cosmetic composition, the composition of the present invention may contain not only the above-described composition of the present invention but also components commonly used in cosmetic compositions. Examples thereof include antioxidants, stabilizers, , Customary adjuvants such as vitamins, pigments and flavoring agents, and carriers.

In addition, the composition may contain 0.001 to 20.0% by weight of the extract of Chengyang leaves based on the total weight of the composition.

In addition, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, the cosmetic composition of the present invention can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

Further, the present invention can provide a pharmaceutical composition for prevention and treatment of acne containing an extract of Ganoderma lucidum as an active ingredient.

In the present invention, "treatment ", as used herein, unless otherwise indicated, refers to reversing, alleviating, inhibiting, or preventing the disease or condition to which the term applies, or one or more symptoms of the disease or disorder , And the term treatment as used herein refers to the act of treating when "treating" is defined as above. Thus, in mammals, "treatment" or "treatment regimen" of acne and related disorders may include one or more of the following:

(1) inhibiting the development of acne and related diseases,

(2) prevent the spread of acne and related diseases,

(3) relieving acne and related diseases,

(4) preventing recurrence of acne and related diseases, and

(5) palliating symptoms of acne and related diseases

The pharmaceutical composition of the present invention may be prepared by using a pharmaceutically acceptable and physiologically acceptable adjuvant in addition to the active ingredient of Chenxu leaf extract as an active ingredient. Examples of the adjuvant include excipients, disintegrants, sweeteners, binders, , A lubricant, a lubricant or a flavoring agent.

In addition, the pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the active ingredient, the extract of Ganoderma lucidum, for administration.

The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile solutions suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

In addition, the pharmaceutical composition of the present invention may contain the extract of Ganoderma lucidum in a pharmaceutically effective amount together with a pharmaceutically acceptable carrier.

The term " pharmaceutically effective amount "in the present invention refers to an amount of an effective ingredient that exhibits relieving, inhibiting, improving and / or curing effects on a disease to be treated. The dosage of the Ganoderma lucidum extract of the present invention varies depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method and disease severity. For example, therapeutically effective dosages can be determined initially using in vitro assays. It will be possible to determine the effective amount for treatment without undue experimentation in the art, and using this information it is possible to more accurately determine useful doses in humans.

The effective amount of the Ganoderma Leaf extract according to the present invention may be 0.5-100 mg / day / kg body weight, preferably 0.5-5 mg / day / kg body weight.

As described above, it is expected that the composition containing the Ganoderma lucidum extract according to the present invention can be substituted for a cosmetic composition for improving acne and an agent for treating acne.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following Examples are only the preferred embodiments of the present invention, and the present invention is not limited by the following Examples.

< Example  1>

curcuma  Manufacture of leaf extract

After the dried turmeric leaves were crushed, 1500 ml of 70% ethanol as a solvent was added to 100 g of the crushed turmeric leaves, and the mixture was extracted at 100 ° C for 20 hours. The resulting filtrate was concentrated under reduced pressure and freeze- Extract powder).

< Experimental Example  1>

curcuma  Antibacterial activity analysis of leaf extracts

The following experiment was conducted to confirm whether the extract of the present invention of Example 1 had the antibacterial activity. The antibacterial activity was measured by a broth dilution method using a 96-well plate. Brain heart infusion broth (BHI broth), which had been sterilized by heating, was added to each well of a 96-well plate. Respectively. Subsequently, the test organism suspension was injected into all wells and incubated at 37 ° C in an anaerobic state. After incubation for 3 days, alamar blue solution was added to each well and cultured for 1 hour. The color development state was observed and the concentration at which no color change from blue to pink was observed was determined as the MIC value. At this time, salicylic acid, trichlorosilane, and trichlorocarban, which are known as materials having a conventional antimicrobial activity, were used as a control group.

MIC concentration measurement for each sample Sample Propionibacterium acnes
(ATCC 6919) MIC (ppm) Ganoderma lucidum extract 15 ~ 45 Salicylic acid 1000 Trichloro line 25 to 50 Trichlorocavan 25 to 55 Purified water -

As a result of the analysis, as shown in Table 1, the extract of the present invention exhibited excellent antibacterial activity as compared with that of salicylic acid, which has been used as an active ingredient in the treatment of acne in the past, and also has a broad antibacterial range of triclosan or trichloro Compared with Kavan, it showed similar effects.

Therefore, it was expected that the extract of Ganoderma lucidum of the present invention could be very useful for the purpose of prevention and treatment of acne.

< Experimental Example  2>

curcuma  Analysis of Anti-inflammatory Activity of Leaf Extracts

The following experiment was conducted to analyze the anti-inflammatory activity of the extract of the present invention. First, a sample of Ganoderma lucidum extract and a control sample (allantoin having an inflammatory sedative activity) were dissolved in purified water, and each of the sample solutions was treated with a solution of hyaluronidase enzyme, calcium chloride (CaCl ₂ ), hyaluronic acid ) And the absorbance change was measured at 585 nm. At this time, the degree of inhibition of enzyme activity of pure ethanol was measured, and the degree of inhibition of the inflammation of the extract sample was measured with a relative inhibition rate. The anti - inflammatory effect was evaluated by the relative inhibition value.

Measurement of Anti-inflammatory Effect of Ganoderma Leaf Extract Sample Active inhibition (%) Ganoderma lucidum extract 121.8 Allantoin 72.8

As a result of the analysis, as shown in Table 2, the extract of Ganoderma lucidum had a relatively higher anti-inflammatory effect than allantoin, which is known to have a high sedative effect when inflammation is induced. From the above results, it was found that the combination of Ganoderma lucidum extract with dermatological compositions can inhibit and alleviate the inflammatory phenomenon accompanying the occurrence of various skin troubles such as acne.

< Experimental Example  3>

curcuma  Antibacterial Activity Analysis of Leaf Extract-Containing Composition

In order to evaluate the antimicrobial effect of the composition containing the extract of Chenghuang leaf extract according to the present invention, a washing soap was prepared with the composition shown in the following Table 3, and the antibacterial activity was analyzed using a paper-disk method. Each composition was dissolved in propylene glycol and diluted with a 10% solution. Paper-disc (diameter 8 mm) was immersed in this solution for the same period of time, taken out, placed on an agar medium inoculated with the test bacteria, and cultured. After 2 days of incubation, the amount of the formation of the developmental depression around the paper-disk was measured and the antibacterial effect of the composition was qualitatively determined. The following Preparation Examples 1 to 3 were prepared by varying the amount of the extract of Ganoderma lucidum extract, and Comparative Example 1 was prepared without adding the extract of Ganoderma lucidum as a control.

Manufacture of cleansing soap containing Ganoderma Leaf Extract Ingredients Production Example 1 Production Example 2 Production Example 3 Comparative Example 1 Palm oil fatty acid 2.0 2.0 2.0 2.0 glycerin 0.5 0.5 0.5 0.5 Titanium dioxide 0.3 0.3 0.3 0.3 EDTA-2Na 0.1 0.1 0.1 0.1 Spices Suitable amount Suitable amount Suitable amount Suitable amount Pigment Suitable amount Suitable amount Suitable amount Suitable amount Ganoderma lucidum extract 1.0 0.1 0.001 - Soap chip (base) Balance Balance Balance Balance Sum 100 100 100 100

Determination of the antimicrobial activity of the composition containing the Ganoderma lucidum extract Sample ATCC 6919 Diameter of growth inhibition zone (mm) Production Example 1 18 Production Example 2 12 Production Example 3 No lowlands formed Comparative Example 1 No lowlands formed

As a result of evaluating the antimicrobial effect of diluted solution of cleansing soap containing the extract of the present invention, the antimicrobial effect was superior to that of the control (Comparative Example 1) as shown in Table 4. In particular, It was found that when added in an amount of more than 1% by weight, it exhibited antibacterial activity.

<Experimental Example 3>

Analysis of acne treatment effect of composition containing Ganoderma lucidum extract

Furthermore, the inventors of the present invention conducted the following experiment to examine whether the composition used in Experimental Example 2 containing the Ganoderma Leaf Extract had the effect of treating acne. That is, in order to evaluate the clinical effects on the prevention and treatment of acne, skin lotion was prepared with the composition shown in Table 6 below, and the test was conducted on the acne holders. More specifically, 30 persons in their twenties who had acne symptoms were treated with the softening longevity (skin lotion) having the composition shown in Table 6 for 2 times a day for 3 weeks to classify the condition of acne according to the criteria in Table 5 below And judged by visual comparison and comparison by photographing. The results are shown in Table 7 below.

Acne treatment efficacy evaluation criteria Rating Skin condition 0 normal One A small number of non-inflammatory cotton 2 Medium irritation without inflammation 3 Inflammatory papilloma 4 Small nodules and inflammatory mediators 5 Spherical nodule 6 Moderate inflammatory nodule 7 Large numbers of inflammatory nodules 8 Severe nodular marsupial 9 Severe cystic and inflammatory nodules

At this time, if the skin condition of the subject of clinical trial is compared with the start point of the experiment, it shows that the grade shows a decrease of more than grade 3, and when the grade of grade 1 or 2 decreases, the grade shows good. Respectively.

Softened longevity containing skin extract of Ganoderma lucidum (skin lotion) Ingredients Production Example 4 Production Example 5 Production Example 6 Comparative Example 2 ethanol 5.0 5.0 5.0 5.0 glycerin 3.0 3.0 3.0 3.0 1,3-butylene glycol 3.0 3.0 3.0 3.0 Polyoxyethylene alkyl ether 0.5 0.5 0.5 0.5 Spices Suitable amount Suitable amount Suitable amount Suitable amount Ganoderma lucidum extract 1.0 0.1 0.001 - Purified water Balance Balance Balance Balance Sum 100 100 100 100

Acne treatment effectiveness evaluation result Ingredients Rating system Great Good no effect worse Production Example 4 13 (65%) 5 (25%) 2 (10%) - 20 (100%) Production Example 5 1 (10%) 9 (45%) 10 (50%) - 20 (100%) Production Example 6 - 2 (10%) 16 (80%) 2 (10%) 20 (100%) Comparative Example 2 - 2 (10%) 15 (75%) 3 (15%) 20 (100%)

As a result of the analysis, the prevention and treatment of acne of the composition for skin according to the present invention showed excellent effects as shown in Table 7 above.

Thus, through the above experimental results, the inventors of the present invention found that the leaf extract of turmeric has excellent antimicrobial activity against bacteria that cause acne, has excellent anti-inflammatory effect, and even when the skin cosmetic composition is actually manufactured and tested It was found that the leaf extract of turmeric can be used as a cosmetic composition for improving acne and a pharmaceutical composition for treating acne.

&Lt; Formulation Example 1 >

Nutrition lotion ( Milk lotion )

The number of softening times was determined according to a conventional method as shown in Table 8 below.

Composition ratio of Lotion with Ganoderma Leaf Extract Composition content (%) Stearic acid 8.0 glycerin 5.0 Squalene 5.0 Propylene glycol 3.0 Self emulsifying glycerin monostearate 2.5 Polyoxyethylene sorbitan monostearate (20 E.O) 1.5 Vaseline 1.5 Spices Suitable amount antiseptic Suitable amount Ganoderma lucidum extract 1.0 Purified water Balance Sum 100

&Lt; Formulation Example 2 &

foundation

The foundation was prepared according to a conventional method as shown in Table 9 below.

Composition ratio of foundation containing Ganoderma Leaf Extract Composition content (%) Titanium dioxide 8.0 Isopropyl myristate 5.0 Talc powder 5.0 Liquid lanolin 3.0 Liquid paraffin 3.0 glycerin 3.0 Bentonite 0.5 Cetostearyl alcohol 0.5 Carboxymethylcellulose sodium 0.3 Colored pigment Suitable amount Spices Suitable amount antiseptic Suitable amount Ganoderma lucidum extract 1.0 Purified water Balance Sum 100

&Lt; Formulation Example 3 >

Mask Pack

A mask pack was prepared according to a conventional method as shown in Table 10 below.

Composition ratio of mask pack containing Ganoderma lucidum extract Composition content (%) Sorbitol 8.0 Polyoxyethylene glycol 1500 6.0 ethanol 5.0 glycerin 3.0 1,3-butylene glycol 3.0 Polyoxyethylene oleyl alcohol ether 1.0 olive oil 0.3 Hyaluronic acid 0.2 Spices Suitable amount antiseptic Suitable amount Ganoderma lucidum extract 1.0 Purified water Balance Sum 100

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (4)

A cosmetic composition for the improvement of acne, comprising the extract of Ganoderma lucidum as an active ingredient. The method according to claim 1,
Wherein the composition contains 0.001 to 20.0% by weight of a calendula leaf extract based on the total weight of the composition. The method according to claim 1,
Wherein the extract is selected from the group consisting of purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, glycerol, propylene glycol, 1,2-butylene glycol, methyl acetate, ethyl acetate, hexane, diethyl ether and dichloromethane Wherein the cosmetic composition is an extract obtained by using at least one selected solvent. A pharmaceutical composition for the prevention and treatment of acne containing an extract of Ganoderma lucidum as an active ingredient.