KR20150055998A - Feed and Feed additives for reducing bad odor of excretion - Google Patents

Abstract

The present invention relates to a feed additive capable of reducing the odor of livestock manure. More specifically, the feed additive can reduce the odor of livestock manure by strengthening the fibrinogen contained in pig feeds. The feed additive is confirmed to increase the growth rate, improve the meat quality, and reduce the odor when added in the feed to be consumed by livestock. The feed additive can have an excellent effect at a low cost to be widely used in the pig feeds.

Description

TECHNICAL FIELD The present invention relates to a feed additive for reducing odor of a livestock manure and a feed comprising the feed additive for reducing bad odor of excretion,

The present invention relates to a feed additive for reducing odor of livestock manure, and more particularly, to a feed additive capable of enhancing fibrin source contained in a feedstuff to reduce the odor generated in a livestock feed.

As a conventional technique for feed additives, Japanese Patent Application Laid-Open No. 2002-238466 discloses a fermented nutrient source in which crushed wheat flour (corn flour) is mixed with one or more kinds of soybean meal, corn, rice bran, And one or two or more microorganisms having chitosan degrading ability (decomposing ability) are added thereto, and aspergillus niger, Aspergillus oryzae (Aspergillus oryzae) ), Bacillus subtilis, and Bacillus licheniformis, and various techniques have been proposed in addition to the technique of using the fermented fermented product (fermented product) as a feed additive. However, the need for technology to reduce the odor of livestock manure more economically and effectively is always required in livestock farms.

In order to solve the problem of supply and demand of excipients such as sawdust and rice hull, livestock manure liquefaction methods have been used, and the "natural circulation type livestock manure recycling system" of Korean Patent No. 10-1107184 and Korean Patent No. 10-1156466 " The present invention relates to a device and a process capable of livestock manure liquefaction in order to solve the problems of the prior art, Storage problem, securing of liquid spraying, etc., and livestock waste is collectively collected and treated in a public way, but the problem of generating odor is serious.

Bio-filter method, activated carbon adsorption method, biochemical wet scrubber method, plasma decomposition method and so on have been proposed as a conventional patented technology for solving the problem of odor. However, all the methods have limitations in continuously and continuously removing odor of high concentration There is a problem that it is very expensive.

Japanese Patent Laid-Open No. 2004-215779 discloses a method of carbonizing livestock at a high temperature (400 to 1,100) and using the carbonized material as a bad odor adsorbent in "deodorant, deodorization method, method of using deodorant and deodorizing structure" There is a problem in that the processing does not require a separate excipient such as sawdust or rice hull, but the cost of carbonization of the livestock.

Conventional technologies for the treatment and reclamation of livestock manure can be applied to a small-scale treatment from an economic point of view, including a liquefaction technique. From the farm farm scale to the large-scale public treatment, the same process is applied at low cost, The development of a method that can be applied flexibly to the scale and fundamentally solving the problem of odor and economically composting is a very important task for solving odor problem and efficient resource recovery of 50 million tons of livestock manure per year .

Under these technical backgrounds, the present inventors have made intensive efforts to accomplish the present invention. It is an object of the present invention to provide a feed additive for odor reduction of livestock manure including anaerobically fermented Italian rice grains with Lactobacillus plantarum KCCM ATCC14917 capable of effectively reducing odor of animal manure .

It is a further object of the present invention to provide a feed which is capable of reducing odors of animal manure while exhibiting good results in feeding and raising livestock containing a predetermined amount of the feed additive.

According to one aspect of the present invention, there can be provided a feed additive for reducing malodor of a livestock manure including an anaerobically fermented Italian rice rabbits with Lactobacillus plantarum KCCM ATCC14917.

According to another aspect of the present invention, there is provided an animal feed reducing odor of livestock manure comprising 0.1 to 1% by weight of an Italian ragas anaerobically fermented with lactic acid bacteria, Lactobacillus plantarum KCCM ATCC14917, based on the total feed weight .

As a result of feeding the feed containing the feed additive of the present invention to livestock, various effects such as improvement of growth rate, improvement of meat quality and reduction of offensive odor have been shown, and since it is economical to obtain an excellent effect even at low cost, It can be spread widely as feed.

Hereinafter, the present invention will be described in more detail.

According to one aspect of the present invention, there can be provided a feed additive for reducing odor of a livestock manure including an anaerobically fermented Italian rice glaze. The above-mentioned lactic acid bacteria strain can effectively use a strain having an excellent effect of reducing the odor of manure by effectively decomposing the Italian rice grains under anaerobic conditions and improving the intestinal environment of the livestock feeding the same. In the present invention, Lactobacillus plantarum KCCM ATCC14917 strain was used.

According to another aspect of the present invention, there is provided a feed for reducing malodor of a livestock manure comprising 0.1 to 1% by weight based on the total feed weight of the anaerobically fermented Italian rice rabbits with lactic acid bacteria. As shown in the following experimental results, when the amount is less than 0.1% by weight, the odor reducing effect is not exhibited. When the amount is more than 1% by weight, the feeding environment of the livestock may change, which is not preferable . It is also important to reduce the odor of the manure in the animal feed, but it is not desirable that it is a condition that interferes with smooth feeding and growth.

In the present invention, the feed additive having the odor reducing effect of the livestock manure and the feed containing the feed additive will be described in more detail with reference to the following examples. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the present invention.

Italian Raglass  Used Anaerobic fermentation  Production of feed

Step 1: Preparation of culture medium of lactic acid bacteria

Lactic acid fermentation broth was prepared to enhance the digestibility and palatability of livestock by enhancing anaerobic fermentation of Italian rice grains. In this example, the strain used for the fermentation of lactic acid bacteria was Lactobacillus plantarum KCCM ATCC14917, which was purchased from the Korean Society for Microorganism Conservation. As a culture medium, industrial cultured lactic acid bacteria culture medium (celltech, Korea) For 36 h for 24 h, and the final bacterial count was measured as 3.8 x 10 ⁹ / g.

Step 2: Italian Ryegrass  Production of fermented feed

Italian rye grass (IRG), packed in a roll form, was preliminarily dried in the sunlight and then cut to a length of about 4-5 cm using a rice straw cutting machine (KA 230, gyeongan agricultural machinery). 500 kg of Italian ragas cut into a certain size was put into a small-scale mixer (DDK801M, Daedong Machinery), 50 L of lactic acid fermentation broth and 150 L of water were added and thoroughly mixed for 10 minutes or more. Sealed in a ton bag and anaerobically fermented for 2 weeks. The fermented anaerobic fermented Italian Ryegrass was able to confirm the fermentation of lactic acid bacteria and measured lactic acid bacteria of 3.8 x 10 ⁸ / g or more.

Step 3. Anaerobic fermentation  Treated Italian Ragras  dry

In order to prevent corruption of Italian rice and promote anaerobic fermentation, the fermentation broth of lactic acid bacteria prepared by the same method as described in step 1 above was used. In addition, the packed Italian ragas were preliminarily dried in the sunlight, cut into a certain size with a rice straw cutting machine, and then 500 kg in weight was added to a small-scale mixer (DDK801M, Daedong Machine). 50 L lactic acid fermentation broth and 150 L Water was added, mixed for at least 10 minutes, sealed in a 700L ton bag with two layers of vinyl, and anaerobically fermented for 2 weeks. The anaerobic fermented Italian Ryegrass was put into a drum type drier (SLH-10, Samhwa Machine) and dried for 28 hours while introducing 50-55 degree hot air. At this time, the moisture content was 11%, and it was possible to produce excellent fermented seaweed and high quality dried Italian rice grains. The lactic acid bacteria of the finally dried Italian raglas were measured to be 3.8 x 10 ⁶ / g.

Step 4: Anaerobic fermentation  Treated Italian Ragras Pellet treatment

The process of producing anaerobically fermented Italian rice pellets by pelletizing, the Italian raglas prepared in step 2 was dried at 50-60 degrees Celsius with a moisture content of about 15% using a hot air drier. The size and uniformity of the crushed particles are very important for the pellets to have a good level of hardness and durability. The dried Italian ryegrass was thus crushed using a hammer mill equipped with a body having a pore diameter of 8 mm. The distribution of the pulverized particles is shown in Table 1.

Particle distribution of crushed Italian rice  Classification Particle Size Distribution of Italian Ryegrass <5m 5-8mm 8-12mm 12-25 mm ratio(%) 28 9 40 23

The pelletizer used in step 4 is a horizontal flat pellet type comprising an extruding part provided in a horizontal form and a roller for generating pressure by pressing the material while rotating on the extruding part. At this time, the compression ratio of the pellet molding die was 1: 5 and the diameter of the produced pellet was 6 mm. Also, in this step, a method of extruding the steam without using a conditioner device for supplying steam was manufactured. The pellet-molded Italian ragas were slightly fermented, but lactic acid bacteria were not detected. Therefore, pelletizing anaerobically fermented Italian rice can increase the effect of supplying fibrin to livestock, but it is thought that feeding of lactic acid bacteria and supply of fermented products will be negligible.

[Example]

The anaerobic fermented Italian rice produced through the above steps was treated under the following conditions (3 treatments).

Control Example 1 Example 2 Basic diet (%) 100 99.5 99.5 Italian Ryegrass Fermented Powder (%) -  0.5  0.5 Wasabi powder (%) - - 0.03

First, feedstuffs containing crude protein 16.5% and lysine 0.87% were used as basic feeds. Anaerobic fermented powder and wasabi powder were prepared according to the above example, And the effect of feeding was investigated. The number of test specimens used in the test was 180 (3 treatments × 5 repetitions × 12 pieces per repetition), and the test money was used for the finishing period (50 to 110 kg). The total duration of the test was 3 months and the type of benefit was free vegetarian. Body weights were measured every 3 weeks.

The crude protein, DE, calcium, phosphorus, lysine and methionine levels of the treatments were the same.

The production process of Italian Ryegrass (IRG) herb is as follows.

Firstly, after the harvesting of the Italian raigas grass heading hay, hay was prepared, crushed to a size of 2 mm or less, and sealed and stored. After that, Italian ryegrass powder was mixed with the compounded diets to prepare diets. The free diet was used for the test diets, and body weight and feed intake were measured every 3 weeks.

Sampling was carried out at intervals of 3 weeks. Slurry samples were collected at -20 ° C. For the sampling, 100 mL of slurry was sampled for analysis of general components and contaminants, and 100 mL of slurry was collected for analysis of odor.

Odor reduction  Effect analysis

- VOC analysis result

Control Example 1 Example 2 Phenols 152.68 (100) 84.31 (55) 90.12 (59)  phenol 5.53 3.53 3.59  p-cresol 147.15 80.77 86.54 Indole 13.76 (100) 9.48 (69) 10.21 (74)  Indole 2.80 2.80 2.82  Scallop 11.00 6.68 7.38

Referring to Table 3, the following effects of the present invention can be confirmed.

First, it can be seen that the amount of all phenols including phenol and p-cresol has decreased rapidly. When the control was converted to 100, 55 of Example 1 and 59 of Example 2 were shown, indicating that the reduction effect was 41 to 45%, and this effect is evenly distributed in both phenol and p-cresol It can be seen that it is observed.

Also, in the case of the indole stream, it is understood that the reduction effect of 31% in Example 1 and 26% in Example 2 is obtained when the control is converted to 100. In the case of indole derivatives, the abatement effect is concentrated on scallol.

Overall, the diets containing the Italian Ryegrass (IRG) anaerobically fermented with lactic acid bacteria according to Examples 1 and 2 according to the present invention showed remarkable reduction effect as compared with the odor reducing effect of ordinary fermented diets Able to know.

The effect of the feed of the feed additive of the present invention when fed to livestock is as follows.

1) The growth rate is improved.

Since the active ingredients contain physiologically active substances (vitamins), growth promoting substances (hormones), and chelating mineral complexes, which promote the growth of livestock, the growth rate of the fattening livestock can be increased by 1520% or more.

2) The resistance to disease is improved by inhibiting the growth of harmful microorganisms such as decaying and degrading microorganisms and pathogenic microorganisms.

Among the active corrosive substances, there are a large amount of antibiotic substances that inhibit the growth of harmful microorganisms, and microorganisms such as actinomycetes, which are mutually antagonistic to these microorganisms, are grown dominantly. Therefore, .

3) Since the generation of odor is reduced in excreted manure, the housing environment is improved.

As a result of the intestinal bacteriostatic action, odor generation is reduced in the manure, and the polyphenolic compounds in the metabolites of the corroded microorganisms are oxidized in air to form quinone compounds. The quinone compounds are ammonia (NH3), hydrogen sulfide (H2S) (Mercaptan), indol, volatile cinnamic acid, volatile organic acid and liver are polycondensation reaction with compounds to immobilize them to nonvolatile corrosive substances, so that odor generation is suppressed and the housing environment is improved, Stress can be reduced.

4) Feed costs are reduced.

As the efficiency of digestion and absorption increases, feed utilization is improved, feed consumption is reduced, and feed costs are reduced.

5) Meat quality is improved.

When minerals and probiotics are fed to finishing pigs and the like, conjugated fatty acids are formed by bio-hydrogenation and the like, and IMF (Internal Muscle Fat) formation is favorable, resulting in excellent formation of marbling Pork meat can be produced.

6) Productivity is improved.

Improvement of positive physiological activity as described above improves the egg production rate in case of chicken, and milk yield and quality in case of cow.

As described above, feeding the feed containing the feed additive of the present invention to livestock resulted in various effects such as improvement of growth rate, improvement of meat quality, reduction of offensive odor, etc., and excellent effects can be obtained even at a low cost Because it is economical, it can be widely used as swine feed.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (2)

Lactobacillus plantarum (Lactobacillus plantarum) Feed additive for odor reduction in livestock manure containing anaerobically fermented Italian rice grains with KCCM ATCC14917. Lactobacillus plantarum (Lactobacillus plantarum) An odor reducing feed for livestock manure containing anaerobically fermented Italian rice, 0.1 to 1% by weight based on the total feed weight, with KCCM ATCC14917.