KR20150024974A - Natural antiseptic composition - Google Patents

Abstract

The present invention relates to a natural antiseptic composition comprising a Nelumbo nucifera extract and a moisturizer containing diol as active ingredients, and wet tissue using the natural antiseptic composition. Since, the natural antiseptic composition has an antiseptic effect against bacteria and fungi equivalent or better than commercial chemical antiseptics, the natural antiseptic composition solves problems of natural plant-derived antiseptics or the mixture thereof such as a limit on the usage range due to a weak antiseptic effect and narrow antibacterial spectrum and a economical problem, thereby overcoming side effects or consumer confidence issues arising from a use of the chemical antiseptics.

Description

[0001] Natural antiseptic composition [

The present invention relates to a preservative composition, and more particularly, to a natural preservative composition which can be applied to give a preservative effect to a sanitary article such as a wet tissue, a cosmetic preparation or a detergent.

Hygiene articles, cosmetics or detergents such as wet tissues and the like contain organic substances, so that it is possible to proliferate microorganisms even after they are produced and transmitted to consumers and during use by consumers. Therefore, in order to suppress the growth of these microorganisms, There may be a variety of processes such as heat sterilization, aseptic packaging, and the like, which may or may not be applicable to such processes, for example, liquid formulations or liquid-containing formulations. It is often difficult or even impossible to inhibit the growth of microorganisms that may occur over a long period of time until completion of use. Therefore, a suitable preservative or a mixed composition thereof should be applied according to the category of the product or the formability of the product, and the kinds and contents of the applicable preservatives are regulated by most laws in each country.

Preservatives with preservative effect are mostly composed of artificially synthesized chemical preservatives which are easy to manufacture, cheap in cost and easy to mass-produce. Typical examples of such preservatives include parabolic acid esters such as Metyl Paraben, Ethyl Paraben, Propyl Paraben, Butyl Paraben, Quaternium-15, Chemicals such as Imidazolidinyl Urea, Diazolidinyl Urea, Phenoxyethanol, Benzalkonium Chloride, Chlorhexidine gluconic acid and Trichlosan, etc. Preservatives can be mentioned.

However, these chemical preservatives inhibit the growth of microorganisms, thereby imparting stability to cosmetics, but also cause irritation to the user's skin. Typical examples are formalin glass-type preservatives. Formalin glass-type preservatives, including diazolidinyl urea and imidazolidinyl urea, have recently been reviewed for safety in the world and their use has been declining. And some wash-off products. These formalin free-form preservatives are known to gradually liberate the formalin group contained in the structure over time, and thus, the liberated formalin is known to have not only cause skin irritation but also cause cancer.

Even preservatives of parabens, which are most safe as conventional chemical preservatives and are commonly used in cosmetics and pharmaceuticals, can cause skin allergies (Andrea Counti et al., Contact Dermatitis, 1997, 37, 35-36) Edwin et al., Toxicology and Applied Pharmacology, 1998, 153, 12-19.) And inducing resistant bacteria. In addition, there is a possibility of new problems such as chronic toxicity and mutagenesis caused by persistent accumulation in the body, which is disallowed within the permitted standards (Shin Dong-hwa, Food Science and Technology, 1990, 23 (4) 68-72).

These side effects of the chemical preservatives cause the users to lower the reliability of the products, ultimately causing consumer complaints about the products to which the chemical preservatives are applied. Therefore, as much as possible, there is an active research on the free radical scavenging system which does not use the chemical preservative and reduce the preservative content in these products. In order to solve the side effects of the existing chemical preservative and to protect the skin, There is a need for natural preservatives that are hypoallergenic and toxic and resistant.

Hinokitiol, Magnoliae extract, Megnonol and Grapefruit seed extract (DF-100) were developed as natural preservatives. However, most of them are limited in antiseptic effect, narrow antimicrobial spectrum, low stability, There are problems such as limited range and economical efficiency.

On the other hand, Nelumbo nucifera is a herbaceous perennial plant belonging to the family Ranunculaceae. It contains vitamin C, vitamin B ₁₂ and amino acids such as asparagine, arginine and tyrosine. It is effective for restoring fatigue and improving skin beauty, (rosemary) and nuciferin (nuciferine) are effective in relieving nervousness and stress. In the private sector, it is known to be used for pneumonia, bronchial asthma, gonorrhea, come.

Korean Patent Publication No. 10-2013-0044429 Korean Patent Publication No. 10-2008-0028391

Accordingly, the present invention provides a natural preservative composition which overcomes the problems of side effects or consumer credibility of a chemical preservative and can overcome limitations of a preservative effect of a natural preservative, a narrow antimicrobial spectrum, limitations of use range, and economy.

The present invention relates to Nelumbo nucifera extract; And monoalkyl glyceryl ether having an alkyl group having 6 to 10 carbon atoms, an alkyl diol having an alkyl group having 5 to 10 carbon atoms, and a glyceryl monoester of a fatty acid having 8 to 12 carbon atoms. Containing at least one diol-containing moisturizing agent selected from the group consisting of:

In addition, the present invention relates to a method for the treatment of Nelumbo nucifera extract; And monoalkyl glyceryl ether having an alkyl group having 6 to 10 carbon atoms, an alkyl diol having an alkyl group having 5 to 10 carbon atoms, and a glyceryl monoester of a fatty acid having 8 to 12 carbon atoms. By weight of at least one diol-containing moisturizing agent selected from the group consisting of water,

Since the present invention exhibits an antiseptic effect equal to or superior to that of a chemical preservative that is commercialized against bacteria and fungi by mixing a diol-containing moisturizing agent with an extract of Nelumbo nucifera , which is a natural preservative exhibiting an antibacterial effect, Or mixtures thereof, overcome the limitations or economics of use due to the narrow antimicrobial spectrum and replace the chemical preservatives, overcoming the side effects or consumer confidence problems associated with the use of chemical preservatives.

FIG. 1 is a photograph showing a test result of a hollow zone test after inoculation of a black fungus bacterium using a wet tissue applied with the natural preservative composition of Example 1 and a commercial wet tissue applied with a chemical preservative in Experimental Example 5. FIG.

Hereinafter, the present invention will be described in detail.

The natural preservative compositions of the present invention comprise Nelumbo nucifera extract; And monoalkyl glyceryl ether having an alkyl group having 6 to 10 carbon atoms, an alkyl diol having an alkyl group having 5 to 10 carbon atoms, and a glyceryl monoester of a fatty acid having 8 to 12 carbon atoms. Or a diol-containing moisturizing agent selected from the group consisting of:

The kite extract may use a plant including leaves, flowers and roots of Nelumbo nucifera , but it is preferable to prepare an extract from a leaf, a flower or a mixture thereof, preferably excluding roots having a high starch content. It is more preferable to use a mixture of 60 to 95% by weight of the lotus leaf extract and 5 to 40% by weight of the lotus extract, rather than using the lotus extract or the lotus leaf extract alone, from the viewpoint of excellent preservative effect. The mixture of the lotus leaf extract and the lotus flower extract may be prepared by mixing the lotus leaf extract and the lotus flower extract, or may be prepared by mixing the lotus leaves and the lotus flower, followed by mixing and extraction.

The extract of the present invention may be an extract of purified water, water, an organic solvent or a mixed solvent thereof. Preferably, the organic solvent may be at least one solvent selected from the group consisting of alcohols having 1 to 4 carbon atoms, hexane, dichloromethane, ethyl acetate, acetone, chloroform and diethyl ether, more preferably methanol, ethanol or methanol And an aqueous solution of ethanol. In addition to the organic solvent, polar solvents such as acetic acid, dimethyl-formamide (DMFO) and dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, decane, Butene, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, Non-polar solvents such as benzene, diethyl ether, diethyl sulfide, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF (Tetrahydrofuran) may be used.

The term extract used in the present invention includes fractions obtained by further fractionating the above extract. That is, not only the water, the lower alcohol having 1 to 4 carbon atoms, the nonpolar solvent, or the mixed solvent thereof, but also the fractions fractionated by the above-mentioned solvents, or the ultrafiltration membrane having various molecular weight cutoff values And fractions obtained.

The method for producing the extract of the present invention can be carried out by, for example, drying and grinding a lotus leaf or lotus flower, and then adding water, an organic solvent or a mixture thereof to the dried sample in an amount of about 1 to 50 times, preferably about 10 to 40 times, At a temperature of from 20 to 100 ° C, preferably from 40 to 60 ° C for about 24 hours, or for from 2 to 4 hours by stirring, hot-water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction or supercritical extraction Or the like, preferably extracting the hot water, and then filtering the extract obtained by filtration, followed by concentration under reduced pressure or drying to obtain the extract of the present invention.

The diol-containing moisturizing agent which is mixed with the kite extract in the natural preservative composition of the present invention and has an enhanced preservative effect is a monoalkyl glyceryl ether having an alkyl group having 6 to 10 carbon atoms, an alkyl having 5 to 10 alkyl groups An alkyl diol, and a glyceryl monoester of a fatty acid having 8 to 12 carbon atoms.

The monoalkyl glyceryl ether is preferably one or more selected from the group consisting of caprylyl glyceryl ether, glyceryl capryl ether and ethylhexyl glycerin, Lt; / RTI > glyceryl ether.

The alkyldiol is preferably at least one selected from the group consisting of caprylyl glycol, 1,2-hexanediol, and pentylene glycol, Lt; / RTI >

The glyceryl monoester of the fatty acid is preferably at least one selected from the group consisting of glyceryl caprylate, glyceryl caprate and glyceryl laurate, Lt; / RTI >

The kite extract and the diol-containing moisturizing agent are preferably mixed in a weight ratio of 3: 1 to 1: 6, more preferably 1: 1 to 1: 3.

In the natural preservative composition of the present invention, the soft extract and the diol-containing moisturizer may be contained in an amount of 0.01 to 100% by weight, preferably 0.1 to 90% by weight, more preferably 1 to 70% by weight.

In the natural preservative composition of the present invention, a mixed solvent of purified water or purified water and a lower alcohol having 1 to 4 carbon atoms can be used as a diluent in addition to the above-mentioned softener extract and a diol-containing humectant. In addition to the diluent, And may further contain a moisturizing agent or preservative other than the component.

The natural preservative composition of the present invention can be prepared by simply mixing raw materials containing the active ingredient at room temperature, preferably by mixing at 15 to 60 캜, and in some cases, The mixture can be sonicated or stirred. Or may be prepared by stirring and then drying.

The natural preservative composition of the present invention can be applied to articles such as sanitary articles such as wet tissues, mouthwashes, shampoos, soaps, shower gels, cosmetics, cleaning agents, disinfectants, diapers and the like, and is preferably applied to wet tissues.

In the article of the present invention, the natural preservative composition is preferably included in an amount of 0.01 to 10% by weight based on the total weight of the article, more preferably 0.05 to 2% by weight based on the total weight of the article. If the natural preservative composition is below the lower limit of the total weight of the product, the antibacterial and antifungal effects are insufficient. If the natural preservative composition exceeds the upper limit, the physical properties of the product and skin irritation may be caused.

The article containing the natural antiseptic composition provided by the present invention may be prepared by mixing the natural antiseptic composition of the present invention with an additional ingredient in the article process which is customarily made in the field, , The impregnated product may be impregnated with the preservative composition of the present invention by spraying the impregnated composition of the present invention to the article to be manufactured, or the impregnated article may be mixed at a certain stage of the article production process.

For example, when the article is a wet tissue, the natural preservative composition of the present invention may be impregnated into the wet tissue through a spraying device or a nozzle device through a spraying or spraying method.

Hereinafter, the present invention will be described in detail with reference to Preparation Examples and Examples. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Preparation Example 1: Preparation of a lotion extract

200 g of dried leaves were extracted with 10 L of ethanol 50% aqueous solution, stirred at 25 ° C for 24 hours in a shaking incubator, filtered through a filter paper, concentrated under reduced pressure, and dried under reduced pressure to obtain a leaflet extract.

Preparation Example 2: Preparation of lotus extract

40 g of the dried lotus flower was added to 2 L of a 50% aqueous solution of ethanol, and the mixture was extracted with stirring at 25 DEG C for 24 hours in a shaking incubator. The mixture was filtered through a filter paper, concentrated under reduced pressure and dried under reduced pressure to obtain a lotus extract.

Production Example 3: Preparation of mixed extract of lotus leaf and lotus

20 g of the dried lotus leaf and 5 g of the dried lotus flower were added to 1 L of a 50% aqueous solution of ethanol, and the mixture was extracted with stirring at 25 ° C for 24 hours in a shaking incubator, filtered through a filter paper, concentrated under reduced pressure and dried under reduced pressure.

Experimental Example 1: Comparison of preservative activity with natural preservatives

The preservative activity of the soft extracts of Production Examples 1 to 3 and the conventionally commercialized natural plant-derived preservative was confirmed for Escherichia coli ATCC 8739.

The commercialized natural plant-derived preservatives include grapefruit seed extract (DF-100), oriental herb extract (EURO-NApre, Sancho, grapefruit, Baekduhong, Mixed extract) and phenoxyethanol as a positive control.

Escherichia coli was inoculated to sterilized water at a concentration of 3.0 × 10 ⁶ CFU / ml, and the soft extract, grapefruit seed extract and Oriental herb extract of the above Preparation Examples 1 to 3 were added in amounts of 5, 10 and 20 wt% , And cultured at 37 ° C for 24 hours.

 After 24 hours, 1 mL was taken from the solution, and the solution was dropped in triplicate on a nutrient agar petri-dish by the spreading method. After incubation for 2 days in a 35 ° C incubator, the number of clusters was counted and multiplied by a dilution factor to make a bacterial count of 1 mL. The results are shown in Table 1 and the unit is CFU / mL.

division 5 wt% 10 wt% 20 wt% Grapefruit seed extract 1.2 × 10 ⁶ 2.8 × 10 ⁴ 3.8 × 10 ² Herbal extract 7.0 × 10 ⁵ 3.2 x 10 ² 3.0 x 10 Production Example 1 1.5 × 10 ⁶ 7.2 x 10 ² 5.2 x 10 Production Example 2 2.5 × 10 ⁶ 5.6 × 10 ⁵ 1.6 x 10 ³ Production Example 3 3.2 x 10 ² 0 0

Experimental Example 2: Confirmation effect of preservative activity according to the mixture ratio of lotus leaf and lotus

Unlike the extracts obtained by extracting the lotus leaves and lotus extracts of Preparation Example 3, the synergistic effects of the preservative synergistic effects and the mixing ratios of the extracts of Preparation Examples 1 and 2 were evaluated through the minimum inhibitory concentration (MIC) evaluation Respectively.

Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, The strain cultivated in 100 mL of Potato dextrose broth in the case of Staphylococcus aureus ATCC 6538 (Nutrient Broth) in 100 mL of Aspergillus niger ATCC 16404 was inoculated with platinum

Mueller Hinton Broth was added to each well of a 96 well plate (96 well plate) in a 96-well plate in an amount of 0.1 mL, and each stock solution was diluted 10-fold to prepare a sample. The mixture of the lotus leaf extract of Preparation Example 1 and the lotus extract of Preparation Example 2 at a weight ratio of 90:10, 75:25, 50:50, 25:75, and 10:90, respectively, and the sample of Preparation Example 3 contained only the first row And then diluted by a 2-fold serial dilution method. Thereafter, a final concentration of Escherichia coli, Pseudomonas aeruginosa Staphylococcus aureus, and Aspergillus niger was added thereto, 5 × 10 ⁶ CFU / mL was added to each well of 96 wells. After culturing at 37 ° C. for 24 hours, the minimum inhibitory concentration (MIC) , And the unit is ppm.

Lotus leaves: lotus Escherichia coli Pseudomonas Staphylococcus Aspergillus 90:10 2500 2500 2500 1250 75:25 1250 1250 1250 625 50:50 18800 18800 18800 9400 25:75 150000 150000 150000 75000 10:90 300000 300000 300000 150000 Production Example 3 1250 2500 1250 625

Experimental Example 3: Confirmatory effect of preservative activity of a mildew extract and a diol-containing moisturizer

The minimum inhibitory concentration of the mixture of the yeast extract of Production Example 3 and the diol-containing moisturizer was confirmed in the same manner as in Experimental Example 2, and is shown in Table 3, and the unit thereof is ppm. Caprylyl glyceryl ether (CGE), ethylhexyl glycerin (EG), caprylyl glycol (CG) and glyceryl caprylate (GC) were used as diol-containing moisturizing agents, And a weight ratio of 1: 1 were prepared and used. As a control, the grapefruit seed extract, oriental herbal extract, and fennel extract of Preparation Example 3 of Experimental Example 1 were used.

division Escherichia coli Pseudomonas Staphylococcus Aspergillus Grapefruit extract 300000 150000 300000 150000 Herbal extract 4700 18800 4700 9400 Production Example 3 1250 2500 1250 1250 Preparation Example 3 and CGE 312 625 312 312 Production Example 3 and EG 625 1250 625 187 Production Example 3 and CG 312 625 312 187 Production Example 3 and GC 312 625 312 187

Experimental Example  4: Yeast extract and Dior  Confirmation of synergistic effect of preservative activity according to mixing ratio of moisturizing agent containing

The minimum inhibitory concentration of the mixture of the yeast extract of Production Example 3 and the diol-containing moisturizer was measured and is shown in Table 4, and the unit thereof is ppm. As the diol-containing moisturizing agent, a mixture obtained by mixing caprylyl glyceryl ether (CGE) with the soft extract of Preparation Example 3 at a weight ratio of 80:20, 50:50, 20:80, 10:90 was prepared and used.

Production Example 3: CGE Escherichia coli Pseudomonas Staphylococcus Aspergillus 80:20 1250 1250 1250 625 50:50 312 625 312 312 20:80 625 625 625 312 10:90 2500 2500 2500 1250

Experimental Example  5: Confirmation of mold growth in wet tissue

A preservative composition prepared by dissolving 0.5 wt% of a mixture of the yeast extract of Preparation Example 3 and caprylyl glyceryl ether in a 1: 1 weight ratio mixture (Example 1) in purified water was mixed with 300 ppm of a natural preservative composition through a spray device Impregnated. As a control group, commercially available wet tissues containing 100 ppm of methyl isothiazolinone (MIT) and 15 ppm of chloromethyl isothiazolinone (CMIT) as a preservative composition were purchased and used.

The wipes were cut into 2 x 7 cm and then incubated at 37 ° C for 24 hours on a plate inoculated with 5 × 10 4 CFU / ml of Aspergillus niger ATCC 16404 to obtain the most frequently occurring contamination The growth of the microorganism fungus was confirmed and is shown in Fig.

As a result, it was confirmed that the natural antiseptic composition of the present invention had antiseptic activity equivalent to that of the MIT and CMIT mixture commonly used as a chemical preservative.

Claims (7)

Nelumbo nucifera extract; And monoalkyl glyceryl ether having an alkyl group having 6 to 10 carbon atoms, an alkyl diol having an alkyl group having 5 to 10 carbon atoms, and a glyceryl monoester of a fatty acid having 8 to 12 carbon atoms. Containing at least one diol-containing moisturizing agent selected from the group consisting of a polyol,
The natural preservative composition according to claim 1, wherein the kite extract is a mixture of 60 to 95% by weight of a lotus leaf extract and 5 to 40% by weight of a lotus extract.
The method according to claim 2, wherein the monoalkyl glyceryl ether is at least one selected from the group consisting of caprylyl glyceryl ether, glyceryl capryl ether, and ethylhexyl glycerin Lt; / RTI >
[3] The method according to claim 2, wherein the alkyldiol is at least one selected from the group consisting of caprylyl glycol, 1,2-hexanediol, and pentylene glycol. Natural preservative composition.
[Claim 3] The method according to claim 2, wherein the glyceryl monoester of the fatty acid is at least one selected from the group consisting of glyceryl caprylate, glyceryl caprate and glyceryl laurate Lt; / RTI >
The natural preservative composition according to any one of claims 3 to 5, wherein the kite extract and the diol-containing humectant are mixed in a weight ratio of 3: 1 to 1: 6.
Nelumbo nucifera ) extract; And monoalkyl glyceryl ether having an alkyl group having 6 to 10 carbon atoms, an alkyl diol having an alkyl group having 5 to 10 carbon atoms, and a glyceryl monoester of a fatty acid having 8 to 12 carbon atoms. By weight of at least one diol-containing moisturizer selected from the group consisting of water,

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