KR20140128110A - External agent for skin comprising extract of cudrania tricuspidata - Google Patents

Abstract

The present invention relates to a preparation comprising an extract of leaves, stems, and roots of Cudrania tricuspidata as an active ingredient for external skin application. According to the present invention, provided is a preparation for external skin application which comprises the Cudrania tricuspidata extract which shows excellent effects for skin whitening, alleviating wrinkles, and moisturizing and a superior effect in treatment of atopic disease.

Description

[0001] EXTERNAL AGENT FOR SKIN COMPRISING EXTRACT OF CUDRANIA TRICUSPIDATA [0002]

The present invention relates to an external preparation for skin containing an extract of Cryptomeria japonica, and more particularly to an external preparation for skin containing a crawfish extract having excellent skin whitening, wrinkle reduction and moisturizing effect and excelling effect for the treatment of atopic diseases .

Cudrania tricuspidata is a small leafy tree belonging to the mulberry tree and cedarwood. It grows in the foothills of the southern part of South Hwanghae province.

Unlike ordinary mulberry trees, the culm trees have long thorns. The edges of the leaves are flat. When the leaves are cut, the white juice comes out like milk.

It is known as a variety of names such as lumber, embroidery, mophong, and gutashi (dialect of Hwanghae province), and its efficacy such as anticancer (uterine cancer, ovarian cancer, esophageal cancer, lung cancer) Since it has been recognized and used as a main material of herbal medicine, its physiological activity is excellent.

Due to its excellent physiologically active function, the extract is mainly used as an additive to food, and no examples have been reported so far as a skin external preparation.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the problems of the prior art as described above, and its object is to provide a cosmetic composition containing a cucurbitan extract having excellent skin whitening, wrinkle reduction, moisturizing effect, Thereby providing an external preparation for skin.

The technical problem of the present invention as described above is achieved by the following means.

(1) A skin external preparation containing an extract of Curcuma longa.

(2) The method according to claim 1,

Wherein the composition further comprises a hydrothermal extract of wild grape leaves.

(3) The external preparation for skin according to claim 2, further comprising a hot-water extract of altitude, oyster, and bracken.

(4) The method according to claim 3,

An external preparation for skin characterized by further comprising a blueberry extract.

(5) The method according to claim 4,

The external preparation for skin according to claim 1, further comprising a lavender extract.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to produce an external preparation for skin containing an extract of Curcuma longa exuding excellent whitening, wrinkle and moisturizing effects and also excelling in the treatment of atopic diseases.

The present invention provides an external preparation for skin containing an extract of Cibumporin.

The present invention is based on the finding that the extracts of the leaves, stems and roots of Cucumis japonica are the major raw materials of cucurbitan, which is produced in Gosung County, Gangwon Province, and the other ingredients of Gosung County are algae leaf, mugwort, oyster, fern, Which is excellent in skin whitening, wrinkle improvement and moisturizing effect and exhibits an excellent effect even in the treatment of atopic diseases, by adding an extract of the present invention to the skin.

In the present invention, leaves, stems and roots are collected in a natural state, washed, cut to an appropriate size, and then a hot-water extract or an ethanol extract is obtained by a conventional method, and the concentrate obtained by concentration under reduced pressure is freeze- However, preferably, the leaves, stalks and roots are obtained by using the following process as a raw material.

First, the leaves, stems and roots are picked up by the pretreatment process and the wilted or discolored leaves are selectively removed. Ethanol is refluxed for 1 to 3 hours with a solvent, filtered and concentrated under reduced pressure to obtain a concentrate of the ethanol extract of each site.

If water is added at a content of less than 0.01% by weight, it is difficult to expect skin whitening, wrinkle improvement or atopic treatment, and if it is added in an amount of less than 10% by weight, By weight, the physical properties of the external preparation may be deteriorated.

In the present invention, an ethanol extract of a wild grape leaf may be further added to supplement and enhance various physiological activity functions which are easily deficient in manufacturing. At this time, ethanol extract of wild grape leaf can be obtained by the same process as the process of producing the extract of cucumber japonica.

The water or ethanol extract of wild grape leaves enhances skin whitening effect. For this purpose, the water or ethanol extract of wild grapevine leaves is added in an amount of 0.001 to 10% by weight based on the total weight of the external skin preparation. If it is added in an amount of less than 0.001% by weight, skin whitening enhancing effect is hardly expected, The physical properties of the external preparation can be deteriorated.

The present invention also provides a method for improving the wrinkle-improving effect of a leaf, a stem, a root, and a blasted leaf, Alternatively, ethanol extracts are prepared and added. The water or ethanol extract solution of these elevations, oyster, and bracken is added in the range of 0.005 to 10 wt% with respect to the weight of the total external preparation composition, and if it is added in an amount of less than 5 wt%, it is difficult to expect a wrinkle- If it exceeds 10% by weight, the physical properties of the final product may be deteriorated.

Preferably, the composition further comprises a water or ethanol extract of blueberry, which is one of the local products grown in the high grade group. The water or ethanol extract of blueberry has a strong effect of enhancing antioxidant activity and preventing skin aging. The water or ethanol extract of blueberry is added in an amount within a range of 0.005 to 10% by weight based on the weight of the total external preparation composition. If it is added in an amount of less than 0.005% by weight, it is difficult to obtain a desired effect. Which may result in deterioration of physical properties.

More preferably, the present invention provides a topical skin preparation to which a water or ethanol extract of lavender, which is one of local specialty products cultivated in a high-grade group, is further added.

Lavender has a rapid absorption in the skin, especially enhances the immune function, and has the therapeutic effect of atopy, especially when it is applied to the skin, it gives the advantage of obtaining the aromatherapy effect. In this case, the water or ethanol extract of lavender is added in the range of 0.001 to 1 wt% with respect to the weight of the total external preparation composition, and if it is added in an amount of less than 0.001 wt% If the amount is more than 10% by weight, the physical properties may be deteriorated.

The external preparation for skin according to the present invention may be formulated by adding an effective amount to various compositions such as cosmetics such as a skin, a lotion, a cream, a foundation, an essence, a gel, a pack, a foam cleansing,

Hereinafter, the present invention will be described in more detail with reference to the following examples and comparative examples. However, it should be understood that the scope of the present invention is not to be construed as limiting the scope of the present invention.

[Experimental Example] Preparation and activity test of Cjimipon extract

After 500g of each leaf, stem, and root of wild goose native to Goseong-gun, 3 times, ethanol was refluxed for 3 hours, filtered and concentrated under reduced pressure to obtain 94g, 53g and 131g of ethanol extract concentrate. Ethanol extracts of each part were treated with acetic acid, ethanol and distilled water to obtain solvent, acetic acid, ethanol and water fractions.

[Antioxidant activity]

The DPPH scavenging activity of the antioxidative effect of the leaves was more than 40% at 200ug / ml of DW extract and 200ug / ml of leaf, and more than 50% at 200ug / ml of ethanol extract of roots (Table 1).

Groups Concentration (ug / ml) DW EtOH EA leaf 25 10.6 ± 1.2 5.4 ± 2.3 9.9 ± 1.9 50 15.1 ± 1.9 12.0 + 4.2 17.2 ± 1.3 100 23.4 ± 4.4 20.0 ± 8.6 28.9 ± 1.9 200 43.2 ± 2.7 24.3 ± 6.4 37.3 ± 1.9 stem 25 1.1 ± 2.8 7.5 ± 1.0 0.9 ± 1.3 50 6.2 ± 0.6 12.9 ± 1.7 2.9 ± 0.5 100 9.9 ± 1.6 23.4 ± 2.2 5.6 ± 0.8 200 15.1 ± 5.2 29.3 ± 4.7 9.6 ± 1.1 Root 25 10.2 ± 0.4 16.8 ± 2.6 9.8 ± 5.5 50 13.4 ± 0.5 21.8 ± 4.0 10.8 ± 9.2 100 18.5 ± 0.5 24.7 ± 2.2 12.1 ± 5.2 200 41.1 ± 0.5 54.1 ± 5.1 28.2 ± 1.3

[Whitening effect]

Melanin production inhibitory effect of B16-F10 cell line was significantly inhibited by 50 ug / ml treatment of acetic acid extract of Cucumber leaf (Table 2).

Groups Concentration (ug / ml) DW EtOH EA leaf 10 117.9 ± 17.0 135.5 ± 19.0 121.8 ± 17.2 100 140.7 ± 18.3 120.8 ± 7.2 60.0 8.0 ^* stem 10 108.3 ± 9.6 124.4 ± 15.1 113.2 ± 2.7 100 122.0 ± 10.5 133.3 ± 10.9 95.0 ± 6.0 Root 10 106.5 ± 10.0 119.1 ± 1.3 158.1 ± 5.1 100 112.9 ± 3.0 103.9 ± 1.7 109.0 ± 9.0

[Wrinkle improvement effect]

In order to confirm the wrinkle improvement effect, collagen synthesis promotion effect of CCD986 or cell line, which is normal skin of human skin, was measured in 10 ug / ml, 100 ug / ml, 10 ug / ml and 100 ug / ml of EtOH extract (*** P <0.001, ** P (0.001), P (0.001), P (0.001), and P <0.01). (*** P < 0.001, * P < 0.05) in the treatment of 10 ug / ml, 100 ug / ml and 10 ug / ml of EA extract.

Groups Concentration (ug / ml) DW EtOH EA leaf 10 117.5 ± 13.7 ^* 139.8 ± 3.8 ^*** 50.8 ± 13.2 100 129.8 ± 4.0 ^*** 153.0 ± 7.3 ^*** 12.6 ± 2.6 stem 10 119.9 ± 4.2 ^*** 95.7 ± 16.9 76.3 ± 7.3 100 127.2 + 24.7 116.1 ± 7.1 ^** 26.3 ± 4.8 Root 10 127.8 ± 7.9 ^*** 81.9 ± 3.3 117.0 + - 11.1 ^* 100 143.4 ± 8.0 ^*** 18.2 ± 6.0 28.4 ± 0.5

As a result of the above experimental results, it was found that the water, ethanol, and ethyl acetate extract of Cucumis japonica have antioxidant, antiinflammatory, whitening effect, wrinkle improving effect and anticancer effect on leaves, stem and roots, Function.

[Example] Preparation of external preparation for skin

Examples 1 to 7 and Comparative Example 1

The ointment for external skin was prepared by mixing each component shown in Table 4 below as shown in Table 5 below.

Composition weight% Example 1 Example 2 Example 3 Example 4 Example 5 Example
6 Example 7 Comparative Example 1 Ethanol Extract
Ethanol Extract
Root ethanol extract
Ethanol extract
Elevated ethanol extract
Oyster ethanol extract
Fern ethanol extract
Blueberry ethanol extract
Lavender ethanol extract
Diethyl sebacate
Nonsense
Polyoxyethylene oleyl ether phosphate
Sodium benzoate
vaseline 5
-
-
-
-
-
-
-
-
8
5
6

Suitable amount
Remaining amount * -
5
-
-
-
-
-
-
-
8
5
6

Suitable amount
Remaining amount * -
-
5
-
-
-
-
-
-
8
5
6

Suitable amount
Remaining amount * 5
-
-
2
-
-
-
-
-
8
5
6

Suitable amount
Remaining amount * -
5
-
-
One
One
One
-
-
8
5
6

Suitable amount
Remaining amount * -
-
5
-
-
-
-
2
-
8
5
6

Suitable amount
Remaining amount * 5
-
-
2
One
One
One
2
2
8
5
6

Suitable amount
Remaining amount * -
-
-
-
-
-
-
-
-
8
5
6

Suitable amount
Remaining amount *

*: Amount to make the total 100% by weight

In order to verify the skin whitening effect on the external skin study made with the above composition, the inhibition of pigmentation and the improvement of skin color were carried out as follows.

Experiment 1. Pigmentation inhibition effect experiment

Twenty healthy men and women were selected as the subjects. Two 7-mm-diameter holes each having a diameter of 7 mm were covered with a 2-line aluminum foil on the lower legs of the arms, and a 60 mJ / Cm &lt; 2 &gt; The bases prepared according to Example 1-7 and Comparative Example 1 were applied to the same row in pairs, three days before irradiation and two times a day till the third week after irradiation. The degree of discoloration of the examples and comparative examples was visually determined, and the degree of inhibition of the pigment deposition compared to the comparative example was evaluated by two steps: effect and difference. The results are shown in Table 3.

Experimental material Effective (persons) No difference (persons) Example 1 15 5 Example 2 16 4 Example 3 15 5 Example 4 18 2 Example 5 15 5 Example 6 15 5 Example 7 19 One Comparative Example 1 0 20

As can be seen from the results in Table 3, the ointment for external use of Examples 1 to 7 exhibited whitening effect for at least 15 out of 20 subjects, and no side effects were observed in the skin, Which is a skin whitening agent having an excellent effect on the skin. In particular, it can be confirmed that the effect is greatest in Examples 4 and 7 in which the extract of wild grape leaves is added.

Experiment 2. Wrinkle improvement effect experiment

The wrinkle-improving effect was evaluated as follows. Eighty women of 30 to 40 years old were randomly divided into eight groups, and the ointments of Examples 1 to 7 and Comparative Example 1 were washed twice daily in the morning and evening, and then an appropriate amount was continuously applied for 2 months around the eyes. The wrinkle improvement effect of each subject was evaluated by visual observation. The experimental results are shown in Table 4 below.

Experimental material Distinct effect (n.) Effective (persons) No difference (persons) Example 1 9 6 5 Example 2 9 6 5 Example 3 9 6 5 Example 4 10 7 3 Example 5 12 9 One Example 6 10 8 2 Example 7 13 7 0 Comparative Example 1 - 5 15

As shown in Table 4, the ointment prepared according to Examples 1 to 7 exhibited wrinkle-improving effect on 15 of 10 subjects and showed no side effects in the skin, Wrinkle improver. Particularly in Examples 5 and 7, distinct improvement effects were observed.

Experiment 3. Atopic symptom relief experiment

30 patients who had atopic disease as a subject were applied to the skin using the base prepared according to Examples 1 and 7 and Comparative Example 1 twice a day for 1 week. The examples and comparative examples were visually judged for each, and the degree to which the example had the symptom relief effect as compared with the comparative example was evaluated in two stages of effectiveness and no difference. The results are shown in Table 5.

Experimental material Effective (persons) No difference (persons) Example 1 6 4 Example 7 9 One Comparative Example 1 0 10

As can be seen from the results in Table 5, the ointment external ointment of Examples 1 and 7 exhibited atopic symptom relief effect for at least 6 out of 10 subjects, and no side effects were observed in the skin.

Claims (5)

External preparation for skin containing extracts from cucumber extracts. The external preparation for skin according to claim 1, further comprising a hydrothermal extract of wild grapese leaves. The external preparation for skin according to claim 2, further comprising a hot-water extract of altitude, oyster, and fern. The method of claim 3,
An external preparation for skin characterized by further comprising a blueberry extract. 5. The method of claim 4,
The external preparation for skin according to claim 1, further comprising a lavender extract.