KR20140080095A - Cosmetic compositions containing bracken hot water soluble extracts and manufacture of the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for infant using bracken hot water extract fermented liquor or a natural substance which additionally includes a green tea hot water extract and a Japanese cypress hot water extract in the bracken hot water extract fermented liquor. More specifically, the cosmetic composition which is manufactured by using the bracken hot water extract fermented liquor or a mixture of the bracken hot water extract fermented liquor, the green tea hot water extract, and the Japanese cypress hot water extract has high moisture, antibacterial activity, or plentiful bubble formation, and an antibacterial activity effect. Therefore, the present invention can be used as an environment-friendly cosmetic composition for infant.

Description

[0001] The present invention relates to a cosmetic composition containing a fermentation broth of bracken water extract and a method for producing the same,

The present invention relates to a cosmetic composition for infants using a natural product containing a hot water extract of green tea and a hydrothermal extract of white tea in a fermentation broth of a bracken water hot water extract or a bracken water hot water extract and a technique for producing the same.

Bracken has the oldest history of plants on the planet. Distributed from tropical to temperate all over the world, about 60 genera, 1500 species are distributed. Among them, 12 genera and 33 species are used for medicinal purposes, mainly rhizomes and foliage. Distribution of Korean bracken is distributed widely in southern and Jeju Island, and can be divided into 8 kinds of hemostasis, 14 kinds of detoxification and 24 kinds of cheongryeol. The medicinal herb contains ground astaxanthin, isoquerecetin, rutin and tannin as flavonoids on the ground (Medicinal Botany Rec. Soc., 2001 ). The fern ( Pteridium aquilinum ) cultivated in Korea belongs to Pteridium ( Dennsteadtiaceae ). The entire plant has hairs, rhizomes (rootstocks) are deeply creeping in the ground, and the leaves are sparse. The harvest time of the bracken is from April to May. Only young leaves are boiled, dried and stored in dried ferns, and then used as food or as food immediately after boiling (Rural Development Administration [RDA], 2011). In one room, the young leaves are called 蕨 (蕨), the root is called 蕨 根 (蕨 根), the fever is lowered, the five is enriched, the poison in the body is released, When jaundice or throat is sick, when fever is high, the stem is roots in autumn, dried in the sun and prescribed as medicine. Nepalese food prepared for the famine is called as a relic, and in general, bracken, goby, bellflower. The effects of polysaccharides and similar mechanisms observed in marine algae such as seaweed and sea tangle in bracken polysaccharides and toxicity of ferns that were not found in fresh ferns were reported .

Green tea has the effect of suppressing various edema including gastric ulcer and gastric mucosal hemorrhage by the effect of inhibiting inflammation and bacterial infection, and has an effect of inhibiting infectious bacteria such as Helicobacter, typhoid, and dysentery and gut bacteria. Contains a lot of antioxidant ingredients to control aging and contains a variety of vitamins (rich in vitamin C, such as 5 times the amount of lemon), helps prevent skin from becoming rough, gives elasticity and maintains moisturizing properties .

Chamaecyparis obtusa ) is an evergreen softwood arboreous tree, belonging to the Quercus mongolica, and is called hinoki or cypress. The strong fragrance of woody wood promotes sterilization, deodorization, blood circulation and metabolism, and is effective for skin beauty and cold. It has the same effect as a forest bath by phytoncide inhaled through the air, and has a fatigue recovery effect. The wood is used as a good wood, and the function of phytoncide is made into a bathtub. When it is used, it is absorbed by the human body through the skin to improve the health. It is effective for improving atopic disease and it is used as wallpaper to prevent the sick house syndrome . Domestic famous movie theaters use the directional effect of natural phytoncide (using the scent of incandescent wood) to clear the head while watching a movie for more than two hours in a closed cinema.

Olive oil is the most unsaturated fatty acid oleic acid (65 ~ 80%), the highest in oil, saturated fatty acid (palmydic acid) and linoleic acid is less than 20%. The saponification material contains 0.5 to 1.3% of phytosterol. The specific gravity is 0.909 to 0.915, the acid value is 0.2 to 6, and the saponification value is 187 to 196, which becomes cloudy at 10 ° C and becomes ointment at 0 ° C. It is light yellow, has no smell, and has a light taste. For industrial use, it is used for soap, fiber lubrication, scalpel, pomade, and for medicine, as a scouring agent, enema, ointment, and injection solvent. The edible oil is mainly used for salads and oil pickling, and contains about 40 kinds of enzymes such as vitamins A, B, C, D, E and K and antioxidants and minerals such as linoleic acid, tocopherol and squalene. Looking at the various effects of olive oil helps prevent aging. It contains simple unsaturated fatty acids and antioxidants such as vitamin E, tocopherol and squalene, which helps prevent aging. Helps maintain healthy skin Oligosaccharides and Vitamin E help prevent skin aging, release naphtha, maintain healthy skin and prevent hair loss and pregnancy. It works to soothe skin's moisturizing and skin troubles. Olive oil has a high antibacterial activity against bacteria and also has a moisturizing ingredient, which is widely used to make cosmetic products for soap skin.

Jojoba oil is obtained from the seeds of jojoba and unlike other vegetable oils, it is the only liquid ester oil that is the natural vegetable oil. An ester mixture of an unsaturated higher fatty acid and an unsaturated higher alcohol, the total number of carbon atoms being 34-50, and the ester having 38-44 carbon atoms occupying more than 95% of the total. The main component is dococenyl eicosanoate (37%) having 42 carbon atoms. Jojoba oil has been used by indigenous people since ancient times as a remedy for skin and has been used for various medicinal purposes. Nowadays, Indians use it as an analgesic to suppress the pain of the wound. Jojoba oil has less oily sensation than other vegetable oils and has a structure of wax ester, a component of sebum, so it is easy to be familiar with skin. By applying a jojoba oil similar to sebum to the skin, it was effective in treating acne by suppressing sebum secretion in the sebaceous gland. In addition, Jojoba oil is easy to absorb on the scalp, gives flexibility of the skin to suppress sebaceous hypersecretion, and also prevents dandruff and promotes hair growth. In addition, the acute toxicity test of the mouse, Jojoba oil, showed no deaths even at doses far exceeding the physiological limits, and it is a highly stable oil with no mucosal irritation or irritation to the skin. Safety, stability, , And is widely used as an oil-based raw material for makeup products such as skin care products, lipsticks, and foundations, mainly in basic cosmetics such as emulsion, cream, body oil, hand lotion and the like.

Coconut oil is the main ingredient of triglycerides of low-intermediate fatty acids such as rutinic acid, myristic acid, and capric acid, which are obtained from the dried copra of the nucleus of coconut. It is a raw material for soap and reduced alcohol and has a melting point of 24 to 27 ° C. Lauric Acid, one of the esters of fatty acids, is a component of human milk that contains 3% of milk fat and butter in natural foods. Coconut oil contains 50% lauric acid, which is suitable for children. It is used as a massage oil because it reduces the allergic reaction. On the other hand, it protects dry skin by preventing skin aging, has an effect of sterilizing wound, and has the effect of eliminating free radicals which cause aging skin and fine wrinkles.

Evening primrose oil has been used by North American Indians for over 1,000 years, as an anti-analgesic medicine and as an external medicine for trauma. Evening primrose oil is composed of 67.5% of linolenic acid, 7.5% of gamma-linolenic acid and 25% of other fatty acids (gamma-linoleic acid is an extremely limited ingredient such as breast milk, evening primrose, black currant seed, and borage) And the efficacy of evening primrose oil is seen as the efficacy of gamma linolenic acid. One of the reasons for the frequent occurrence of atopic dermatitis is due to the lack of gamma linolenic acid. In the UK, Prestol University, the infant of breastfeeding does not eat breast milk and lack of gamma linolenic acid in breast milk causes atopic dermatitis. Oil has been reported to be very good treatment effect.

Perilla seed oil is extracted from lupine and plant perilla and contains 60 ~ 70% linolenic acid (omega 3 fatty acid) indispensable to human body such as alpha linolenic acid. In the world, the content of linoleic acid is known to be richest in perilla oil, and linolenic acid plays an important role in keeping the skin healthy by preventing evaporation of water. In addition, rich in vitamins E and F to enhance the regenerative power of the skin to help regenerate damaged skin and protect the skin.

The skin, which is distributed in the outermost part and the largest area of the human body, protects the human body from external physical and chemical stimuli and performs important functions such as moisture control, immune reaction, body temperature control and waste product secretion. The skin is composed of epidermis, dermis, and subcutaneous fat layer. The outermost layer of epidermis is called the stratum corneum and plays an important role in skin moisturization. The stratum corneum suppresses the loss of water and electrolytes through the skin and plays a key role in maintaining the normal physiological activity of the skin by protecting against external stimuli such as microorganisms and ultraviolet rays. When a moisturizer is developed by applying a natural moisturizing factor or a polyol such as glycerin or butyleneglycol, the skin moisturizing agent is used to promote the differentiation of keratinocytes, There are many ways to develop moisturizers. On the other hand, development of a skin moisturizing agent using a natural material has a small adverse effect on the skin, and recently, the value of development as a cosmetic raw material is further increasing as consumers' response to cosmetics using natural materials increases.

The skin of the infant is easily dried because the acidic protective film of the skin is undeveloped and is vulnerable to external bacteria and the sebaceous gland fails to function. In addition, it is characterized by cold and shock due to thin subcutaneous fat layer. The skin surface area is twice as large as that of adult skin, and more amount of water is evaporated through the skin and the amount of substance absorbed into the skin is also high. Care should be taken to maintain the cleanliness of 0-3 months after birth, and 6 months after birth, sebaceous glands are developed and care should be taken to moisturize. Since the pH of the skin is generally higher than normal in infancy, it is more important to use a neutral detergent rather than an alkaline soap to keep it clean.

Accordingly, the present inventors have developed an eco-friendly cosmetic composition using a variety of essential oils and natural extracts obtained by mixing a hot water extract of green tea and a hydrothermal extract of a green tea into a fermentation liquid of bracken water extract or hot water extract of bracken, Functional and antimicrobial activity, it is expected to be useful as a non-irritating eco-friendly cosmetic composition for infants.

An object of the present invention is to provide a cosmetic composition containing a fermented liquid of brackish water hot-water extract as an aqueous stream.

It is another object of the present invention to provide a cosmetic composition containing, in addition to the fermentation broth of bracken water hot-water extract, the same proportion of green tea hot water extract and whitening hot water extract as an aqueous stream.

It is also an object of the present invention to provide a method for producing a cosmetic composition for lotion.

In addition, an object of the present invention is to provide a process for producing a cosmetic composition for cleanser.

In order to accomplish the above object, the present invention provides a cosmetic composition comprising a fermented liquid of brackish water hot-water extract as an aqueous stream.

The present invention also provides a cosmetic composition comprising, in addition to a fermentation broth of a bracken water extract, a green tea hot water extract and an albite hot water extract in the same proportion as an aqueous stream.

The present invention also provides a process for producing a cosmetic composition for lotion.

In addition, the present invention provides a process for producing a cosmetic composition for cleanser.

The antimicrobial activity, the water retention capacity as a lotion and the cleanser of the cosmetic composition prepared by using the fermentation liquid of the bracken water hot water extract of the present invention or the natural extract obtained by mixing the hot water extract of green tea and the hydrothermal extract of the green tea, It is possible to use it as an environmentally friendly cosmetic composition for infants.

1, A photograph showing the distribution of microorganisms in rice soot before and after fermentation in rice soot;
A and B: microorganisms before rice flour fermentation; And
C and D: Microorganisms after rice hot fermentation.
Figure 2 is a grass Pythium pathogens A photograph showing the antifungal effect of selected microorganisms after fermentation on graminicola ;
A: Paenibacillus polymyxa ;
B: Lactobacillus plantarums ; And
C: Lactococcus latis.
Fig. 3 is a graph showing the effect of the Rhizoctonia solani A photograph showing the antifungal effect on the antifungal effect of selected microorganisms after fermentation on AG-1 (1B);
A: Paenibacillus polymyxa ;
B: Lactobacillus plantarums ; And
C: Lactococcus latis.
Figure 4 FIG. 3 is a photograph showing the hue of the cleanser of the present invention and the shape of the foam generated using the cleanser; FIG.
Cleanser B: a cleanser prepared by using a fermentation broth of bracken water extract;
Cleanser C: Cleanser prepared by using hot water extract of green tea;
Cleanser D: a cleanser prepared by using a hydrolyzate extract; And
Cleanser E: A cleanser prepared by mixing 1: 1: 1 mixture of fermented broth, green tea hot water extract, and white hot water extract.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition comprising a fermentation liquid of brackish water hot-water extract as an aqueous stream.

The fermentation broth of the bracken water extract

1) preparing rice flour;

2) preparing a hot water extract of bracken; And

3) mixing and culturing the rice husk of step 1), the hot water extract of brackish water of step 2), and the suspension of bacteria derived from rice soap, but the present invention is not limited thereto.

Preferably, the rice bacteria-derived bacteria are lactic acid bacteria isolated from rice flour, and Lactobacillus plantarums (DSM 21380) and Lactococcus latis (KFCC 11510P), but is not limited thereto.

The fermentation broth of the bracken water extract is preferably dried by a method selected from the group consisting of freeze drying, vacuum drying, dehumidification drying and hot air drying, but is not limited thereto.

In addition, the present invention provides a cosmetic composition comprising a green tea hot water extract and a white hot water extract as an aqueous stream in addition to a fermentation liquid of a bracken water hot water extract.

The fermentation broth of hot water extract, green tea hot water extract, and white hot water extract are preferably in a ratio of 1: 1: 1, but are not limited thereto.

Also, the cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, , Soaps, cleansing foams, cleansing lotions, cleansing creams, lotions and body cleansers. When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component When the formulation of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component. Examples of the solvent include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate Glycolate, glycerol aliphatic ester, polyethylene glycol, or fatty acid esters of sorbitan. When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used. When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may comprise a composition selected from the group consisting of water-soluble vitamins, useful vitamins, high molecular weight peptides, polymeric polysaccharides and sphingogly lipids. In addition to the above components, Antimicrobial agents, antioxidants, plant extracts, pH adjusters, alcohols, coloring matters, perfumes, blood circulation accelerators, coolants, antiperspirants, antioxidants, emollient agents, surfactants, organic and inorganic pigment organic powders, , Purified water, and the like may be blended. In addition, the cosmetic composition of the present invention can be prepared into any formulation in which the cosmetic composition is usually prepared, and can be, for example, an emulsion, a cream, a lotion, a pack, a foundation, a lotion, a serum, a cleanser and a hair cosmetic.

In addition,

1) preparing an aqueous stream;

2) preparing a planetary flow;

3) heating the oil phase of step 2) to 60 to 80 ° C; And

4) mixing and stirring the oily oil heated in step 3) with the aqueous stream of step 1), and a method for producing the cosmetic composition for lotion.

The oil phase of step 2) is at least one selected from the group consisting of linseed oil, pudded oil, sunflower oil, poppy oil, perilla seed oil, sesame seed oil, rape oil, rice bran oil, pumpkin seed oil, calendula oil, soybean oil, rice bran oil, It is preferably selected from the group consisting of oil, grape seed oil, jojoba seed oil, and olive oil. It is more preferably one or more selected from the group consisting of jojoba seed oil, perilla seed oil, evening oil, and olive oil. It is not limited.

In the above description, the aqueous stream means an aqueous raw material which is polarized due to the distribution of electrons in the molecule and therefore dissolved well in water.

In the above, the oil phase is a non-polar oil-soluble raw material having a property of being insoluble in water.

In addition,

1) preparing a planetary flow;

2) adding liquid water, algae and sugar to the oily water of step 1) to prepare a liquid type soap base;

3) aging the liquid type soap base of step 2); And

4) dissolving the aged liquid type soap base of step 3) with the fermented broth of hot water extract of bracken water as a solvent, thereby producing a cosmetic composition for cleanser.

The oil phase of step 1) is preferably selected from the group consisting of corn oil, cottonseed oil, peanut oil, palm oil, castor oil, coconut oil, evening oil, olive oil, soybean oil, rice bran oil, palm oil, , And olive oil, but is not limited thereto.

The aging step of step 3) is preferably aged for 2 to 6 weeks, but is not limited thereto.

The aging is preferably carried out at a temperature of 18 to 40 캜 in a sealed state, but is not limited thereto.

In addition, the present invention provides a method for producing a cosmetic composition for cleanser comprising, as a solvent, a green tea hot water extract and a white hot water extract in addition to the fermented hot water extract of step 3).

The fermentation broth of hot water extract, green tea hot water extract, and white hot water extract are preferably in a ratio of 1: 1: 1, but are not limited thereto.

In a specific embodiment of the present invention, the present inventors prepared fermented hot water extract of bracken water, hot water extract of green tea, and hydrothermal extract of white tea, and using a mixed solution of 1: 1: 1 of each of them or a mixture thereof, (See Table 2 and Table 5). The manufactured lotion showed antimicrobial effect (see Table 7 and Table 8) and excellent moisturizing effect (see Table 6) with excellent water retention ability, and the produced cleanser showed abundant foaming effect and excellent antibacterial effect See Table 9).

Accordingly, the lotion and the cleanser prepared by using the fermentation liquid of the bracken water hot water extract, the fermentation liquid of the bracken hot water extract, the green tea hot water extract and the mixed hot water extract of the green tea according to the production method of the cosmetic composition of the present invention are environmentally friendly, It can be confirmed that it can be used as a composition.

The present invention can be more specifically understood by the following examples, and the following examples are intended to illustrate the present invention and are not intended to limit the scope of protection of the present invention.

< Example  1> Bracken Hot water extract  Manufacture of fermentation broth

<1-1> Bracken Hot water extract  Produce

The present inventors have produced a brackish water hot water extract.

Specifically, juvenile fern (cultivated: Jeju city line) collected in April to May was boiled for 15 minutes in water at 90 ° C at a rate of 1.0 kg of bracken per 1.0 L of water. The boiled fern was dried and used as a processed product. In addition, hot water extract of brackish water remained as waste by-products was prepared. The prepared extract solution was kept at 90 캜, and the same amount of balsam was again added to boil it in the same manner, and then the balsam was separated. A total of 4 ferns were placed in boiling water at 90 ° C. After the fourth fern was separated, the final fern extract was collected. The collected extracts were stored in a sealed container and stored at -20 ° C in a freezer.

In addition, the brackish water extract was freeze-dried at -70 ° C for 24 hours with a freeze dryer (PVTFD10A, Ilshin, Korea) and dried for 72 hours to be powdered. The yield of the average powder is 4.85% (weight of powder obtained / weight of sample taken). The powdered samples were stored in a sealed container and stored in a freezer.

<1-2> Selection and identification of fermenting bacteria

The present inventors selected and identified fermenting bacteria for fermentation of bracken water extract from rice flour.

Specifically, 1000 g of sterilized water was added to 500 g of pesticide-cultivated rice (Sotomi, Uljin, Gyeongbuk, Ulsan, Gyeongbuk, Korea), and rice flour was prepared by washing rice twice. 10% of sugar was added and mixed in the sterilized bottles, and the mixture was incubated at 28 ° C in a thermostat at 1000 rpm for 7 days to prepare fermented rice flour. In order to confirm the distribution of microorganisms before and after the fermentation of the rice flour, the rice flour and the fermented rice flour were applied to the TSA medium. As a result, it was confirmed that the distribution of microorganisms before and after the rice flour fermentation was different ). Then, the fermentation in order to separate, in a microorganism tteumul rice, the number of sterilized fermented rice tteumul was diluted to 10 ^-1 to 10 ^-5 put in a test tube containing 9 ml each, of the 10 ^-3 dilution, ^{10 4} , and 10 ^-5 were extracted, and the cells were plated on TSA (Tryptic Soy Agar) medium and cultured in a thermostat at 28 ° C for 48 hours. Among the microorganisms originated from the rice cultivated in the TSA medium, colonies grown in different shapes and colors were identified by naked eyes, and then 12 different microorganisms were isolated by single cell sorting by dividing into new TSA medium. Pythium graminicola , Pythium ( Pythium graminicola) , and Pythium graminicola , which were isolated from PDA (potato dextros agar) ㎕imum , Rhizoctonia cerealis , Rhizoctonia solani AG-1 (1A), Rhizoctonia solani AG-1 (1B) and Rhizoctonia solani AG-2-2.

As a result, two bacteriumes showing an antifungal effect were selected (FIGS. 2 and 3). The DNA of the isolated bacterium was extracted according to the method described in Ausubel et al. (1987) The sequences were analyzed using universal primer 38 r: 5'-CCG GGT TTC CCC ATT CGG-3 'and 72f: 5'-TGC GGC TGG ATC TCC TT-3' (Gurtler and Stanisich, 2001).

As a result, the bacteria showing the antifungal effect against grass pathogens were Lactobacillus plantarums (DSM 21380) and Lactococcus latis (KFCC 11510P).

<1-3> Bracken Of hot-water extract  Fermentation

The present inventors have produced a fermentation broth of brackish water hot water extract by using fermenting bacteria isolated from rice flour and rice flour.

Specifically, 500 ml of rice flour was added to 2.0 L of a brackish water hot water extract containing 1 to 10% of sugar, and then the microorganisms Lactobacillus plantarums and Lactococcus strains isolated in Example <4-1> latis was added and cultured at 20 DEG C for 40 days. The resultant fermented broth of hot water extract of bracken was freezed at -70 ° C for 24 hours, and then incubated for 72 hours in a freeze dryer (PVTFD10A, Ilshin Lab, Korea) Dried, powdered, and stored in a freezer in a sealed container.

< Example  2> Green tea and cottonwood Hot water extract  Produce

<2-1> Green tea Hot water extract  Produce

The present inventors prepared a hot water extract of green tea.

Specifically, the leaves and stems produced in this year of green tea plants grown by organic cultivation (Jeju Seoguipo Seoguipo Seogwipo Seongpyo-si Seongpyeong-si Seogwipo-si Seongpyeong-myeon) were harvested at a rate of 2.0 kg of plant per 10.0 liters of water, Hour). The hot water extract was collected by heating until the amount of water reached 5.0 L. The collected hot water extracts were stored in a freezer at -20 ° C in a sealed container.

&Lt; 2-2 > Hot water extract  Produce

The present inventors have produced a hydrothermal extract of P. monocytogenes.

Specifically, the leaves and stems produced in this year of the unicorn tree (organic cotton seedlings of Seongpyo-si Seogwipo City, Seogwipo City, Seogwipo City, Jeju Island) were harvested and treated in the same manner as the hot water extract of green tea of Example <2-1> Were collected.

< Example  3> High moisture  Baby lotion manufacturing

The present inventors produced a highly moisturizing lotion for infants using a fermented broth of hot water extract of bracken water, hot water extract of green tea, a hydrothermal extract of pure white water and a mixed solution thereof as an aqueous raw material.

Concretely, 67.6 g of aloe vera gel and 2.0 g of aloe vera gel were added to each other in a mixed solution of purified water (control), bracken water hot water extract, green tea hot water extract, , And the mixture was heated to 70 deg. Thereafter, olive oil, jojoba seed oil, perilla seed oil, and evening oil were added to other beaker in the same composition as in Table 1, respectively. To each of the beaker containing the aqueous raw material, a combination of different oils was added, and the mixture was stirred at 200 rpm for 1 minute. 0.4 g of xanthan gum was added to each mixture, and then the mixture was stirred at 200 rpm for 2 minutes.

As a result, lotions A, B, C, D and E lotions were prepared (Table 2).

Material 1 Oil type 30.0g Materials 1 and 2 were mixed and stirred (2000 rpm, 1 minute), and then material 3 was added, followed by final stirring (2000 rpm, 2 minutes) Material 2 Aqueous stream 69.6 g Material 3 Emulsifier 0.4 g Lotion containing natural extract for high moisturizing Lotion A Lotion B Lotion C Lotion D Lotion E Aqueous stream Purified water Bracken
Hot water extract
Fermentation liquid green tea
Hot water extract Textiles
Hot water extract Mixed solution (1: 1: 1) color white
(White) greenish brown
(Greenish brown) light brown
(partridge) redish brown
(Reddish brown) yellowish
brown
(Beige)

< Example  4> High moisture  Baby cleanser manufacturing

<4-1> Preparation and selection of liquid soap base

The inventors of the present invention manufactured liquid type soap bases of various oily types and confirmed the foam keeping effect in order to manufacture a cleanser for high moisture.

Specifically, the liquid type soap base was prepared by changing the kind or mixing ratio of the oily type as shown in Table 3, and then aged for 6 weeks in a shade at 15 ± 2 ° C in a zipper bag. The aged soap base and purified water were mixed at a ratio of 2: 8 to prepare a liquid soap base. Thereafter, the liquid type soap bases A, B, C, and D were placed in a conical tube in an amount of 5 ml each, and the mixture was stirred at 200 rpm for 30 seconds with a VOLTEX. After 1 minute, The petri dishes were foamed using a 150 ml bubble container containing bases A, B, C and D pumped. Six times in total, and after 30 minutes, the degree of bubble retention was observed to confirm the degree of bubble retention.

As a result, the liquid type soap base (B), the liquid type soap base (C) and the liquid type soap base (D) produced abundant foam (A <B <C <D) as compared with the conventional liquid type soap base Soap base D had the best foam formation (Table 4).

Liquid type soap base A B C D Coconut palm oil, castor oil olive oil Olive oil, coconut oil, evening meal oil Olive oil, coconut oil, evening meal oil Characteristics of foam
And persistence Small bubbles formed in small amounts Small bubbles formed in large quantities Large bubbles form, but not retained (about 1/2 of 30 minutes left) Large bubbles are formed in large quantities and held for a long time (about two-thirds after 30 minutes) Amount of foam (ml) 14.0 ± 3.5 23.3 ± 2.6 31.7 ± 2.6 32.5 ± 2.6

<4-2> High moisture  Baby cleanser manufacturing

The present inventors prepared a cleanser for hyper-moisturizing infant using the liquid type soap base D of the above Example <4-1>, the fermentation liquid of the bracken water extract, the green tea hot water extract, the white hot water extract and the mixed solution thereof as an aqueous raw material.

Specifically, a mixed solution obtained by mixing 1: 1: 1 of fermented broth of bracken water extract, green tea hot water extract, white hot water extract, and bracken water hot water extract, green tea hot water extract and white hot water extract was used as a solvent, -1 &gt; liquid soap base D.

As a result, baby cleansers A, B, C, D and E were prepared for high humidity (Table 5).

Cleanser A Cleanser B Cleanser C Cleanser D Cleanser E menstruum Purified water Bracken
Hot water extract
Fermentation liquid green tea
Hot water extract Textiles
Hot water extract Bracken water hot water extract fermentation liquid + green tea hot water extract + white water hot water extract = 1: 1: 1 pH 9.29 7.27 7.45 7.55 7.01 color white
(White) dark-brown
(Dark brown) brown
(Brown) redish brown
(Reddish brown) deep-brown
(Dark reddish brown)

< Experimental Example  1> High moisture Baby  Check the effectiveness of the lotion

<1-1> Measurement of volume size of lotion

The present inventors measured the volume of the lotion produced in Example 3.

Specifically, the lotion (A) showing the average disposition is set as a standard on the basis of the spreadability of the lotion brought into contact with the skin (unfavorable result), and then the lotion (B), the lotion (C), the lotion ) And lotion (E) were dipped in a petri dish at a low temperature for 7 days after aging, and the volume was measured (Table 6).

<1-2> Antibacterial activity of lotion containing natural extract

The present inventors evaluated the antibacterial activity of lotions (A), (B), (C), (D) and (E) prepared in Example 3.

Specifically, the Korean agricultural culture collection (KACC), the staphylococcus strain aureus was incubated at (Staphylococcus aureus: pathogen A) and Klebsiella pneumoniae: The optimum temperature for the pathogen was transferred to (pneumoniae pathogen B) each TSA (Tryptic Soy Agar) medium (30 ℃ pathogen A:: 36 ℃, pathogens B) . A suspension of 1.0 × 10 ⁶ cfu / ml was prepared by adjusting the pathogen A cultured at 36 ° C. for 24 hours with sterilized water, and 50 μl of a suspension of pathogen A was added to the TSA medium. After 30 minutes, 10 μl of each of purified water, hot water extract of green tea, hot-water extract of green tea, and hydrolyzed extract of hot water, and mixed solution thereof was placed in the medium on which the pathogens were smoothed. When the solution was absorbed into the medium, Respectively. The lotion solution of Example 3 prepared by using purified water, a fermented hot water extract of green tea, a hot water extract of green tea, a hydrocolloid extract, and a mixed solution thereof was placed on the pathogen-coated medium at intervals of 10 μl each, When the solution was absorbed into the medium, it was observed by culturing for 24 hours at 36 ° C (the temperature of pathogen A) (antimicrobial activity against pathogen A). In addition, the pathogen B was also tested for antimicrobial activity in the same manner as pathogen A, and the incubation temperature was 30 ° C (antimicrobial activity against pathogen B).

As a result, the antibacterial effect was observed in all of the lotion liquid of Example 3 of the present invention (Table 6).

Lotion A Lotion B Lotion C Lotion D Lotion E Aqueous stream Purified water Bracken
Hot water extract
Fermentation liquid green tea
Hot water extract Textiles
Hot water extract Mixed solution (1: 1: 1) volume
size 4.0mm 3.0 mm 2.5 mm 3.5mm 4.5mm Antimicrobial activity against pathogen A - ++ ++ ++ ++ Antimicrobial activity against pathogen B) - + ++ + ++

<1-3> Evaluation of water holding ability

The present inventors evaluated the water holding ability of the lotions (A), (B), (C), (D) and (E) prepared in Example 3.

Specifically, a lotion (A) prepared using purified water as a control group was used, and as a test group, lotion (B) prepared using a fermentation liquid of bracken water hot water extract, lotion (C) prepared using a green tea hot water extract, The lotion (D) prepared by using hot water extract was weighed at 30 ° C for 3 days under dry conditions of 30% moisture content and twice at wet condition of 60% moisture content (10 o'clock and 18 o'clock) (The weight of the water evaporated from the lotion = (n + 1) -n, n: the weight of the lotion measured in the previous step). Each experiment was repeated three times.

As a result, as shown in Table 7 and Table 8, in both the dry condition (Table 7) and the moist condition (Table 8), the amount of the evaporated water of the lotion containing the natural extract was significantly smaller than that of the control lotion A Especially, Lotion B, which was prepared by using fermentation broth of bracken water extract, was the most effective.

time Lotion A Lotion B Lotion C Lotion D Purified water Bracken water extract
Fermentation liquid green tea
Hot water extract Textiles
Hot water extract 1 day morning (00 hours) ㅡ ㅡ ㅡ ㅡ 1 day afternoon (08 hours) 0.113 0.009 0.011 0.043 2 days morning (24 hours) 0.247 0.012 0.017 0.056 2 PM (32 hours) 0.150 0.004 0.012 0.061 3 days morning (48 hours) 0.075 0.0010 0.027 0.060 3 days afternoon (56 hours) 0.078 0.004 0.018 0.046

time Lotion A Lotion B Lotion C Lotion D Purified water Bracken water extract
Fermentation liquid green tea
Hot water extract Textiles
Hot water extract 1 day morning (00 hours) ㅡ ㅡ ㅡ ㅡ 1 day afternoon (08 hours) 0.016 0.003 0.006 0.009 2 days morning (24 hours) 0.038 0.009 0.010 0.012 2 PM (32 hours) 0.008 0.002 0.002 0.005 3 days morning (48 hours) 0.014 0.009 0.009 0.012 3 days afternoon (56 hours) 0.009 0.002 0.002 0.006

< Experimental Example  2> High moisture  Baby Cleanser of  Check the effect

<2-1> High moisture  Baby Cleanser of Foam amount  evaluation

The present inventors evaluated the amount of foam of the cleanser prepared in Example 4.

Specifically, the cleanser of Example 4 prepared using a fermented broth of bracken water extract, a green tea hot water extract, a white hot water extract, and a mixed solution thereof was placed in a conical tube of 5 ml each, and the resultant was vortexed at 200 rpm for 30 seconds , And the amount of foam was confirmed at a point of 1 minute after agitation (Table 9 and Fig. 4).

<2-2> High moisture  Baby Cleanser of  Evaluation of antibacterial activity

Specifically, the Korean agricultural culture collection (KACC), the staphylococcus strain aureus was incubated at (Staphylococcus aureus: pathogen A) and Klebsiella pneumoniae: The optimum temperature for the pathogen was transferred to (pneumoniae pathogen B) each TSA (Tryptic Soy Agar) medium (30 ℃ pathogen A:: 36 ℃, pathogens B) . A suspension of 1.0 × 10 ⁶ cfu / ml was prepared by adjusting the pathogen A cultured at 36 ° C. for 24 hours with sterilized water, and 50 μl of a suspension of pathogen A was added to the TSA medium. After 30 minutes, 10 μl of each of purified water, hot water extract of green tea, hot-water extract of green tea, and hydrolyzed extract of hot water, and mixed solution thereof was placed in the medium on which the pathogens were smoothed. When the solution was absorbed into the medium, Respectively. The cleanser of Example 4 prepared by using the purified water, the fermented hot water extract of green tea, the hot water extract of green tea, the hydrocolloid extract, and the mixed solution thereof was placed on the pathogen-coated medium at intervals of 10 쨉 l each, Once absorbed into the medium, it was observed for 24 hours at the appropriate temperature of the pathogen (antimicrobial activity against pathogen A). In addition, the pathogen B was also tested for antimicrobial activity in the same manner as pathogen A, and the incubation temperature was 30 ° C (antimicrobial activity against pathogen B).

As a result, it was confirmed that all of the cleansers of Example 4 of the present invention had an antibacterial effect, and in particular, the cleansers (B) and (E) prepared using the fermentation broth of a bracken water hot- (Table 9).

Cleanser  A Cleanser  B Cleanser  C Cleanser  D Cleanser  E menstruum Purified water Bracken
Hot water extract
Fermentation liquid green tea
Hot water extract Textiles
Hot water extract Bracken water hot water extract fermentation liquid + green tea hot water extract + white water hot water extract = 1: 1: 1 pH 9.29 7.27 7.45 7.55 7.01 color white
(White) dark-brown
(Dark brown) brown
(Brown) redish brown
(Reddish brown) deep-brown
(Dark reddish brown) bubble
size Small size bubbles Small bubbles occur abundantly in abundance Large bubbles occur in large quantities Large and small bubbles occur abundantly Large and small bubbles occur abundantly bubble
maintain 1/2, the foam quickly disappeared. 2/3, long-lasting foam 2/3, long-lasting foam 1/2
Foam quickly disappears 2/3. Bubbles last longer Bubble amount evaluation
(ml) 25.5 ± 4.4 26.7 ± 2.6 32.5 ± 2.7 36.7 ± 2.6 36.7 ± 4.1 Antimicrobial activity against pathogen A - ++ ++ +++ +++ Antimicrobial activity against pathogen B - ++ + + ++

Claims (9)

A cosmetic composition comprising a fermentation broth of hot water extract of bracken as an aqueous stream.
The fermentation broth of the bracken water extract of claim 1
1) preparing rice flour;
2) preparing a hot water extract of bracken; And
3) The cosmetic composition is prepared by mixing and culturing the rice husks of step 1), the bracken water extract of step 2), and the bacterial suspension from rice soap.
A cosmetic composition comprising the same amount of the green tea hot water extract and the white hot water extract as the aqueous mixture in addition to the fermentation liquid of the bracken hot water extract according to claim 1.
The cosmetic composition according to claim 1 or 3, wherein the cosmetic composition is a lotion or a cleanser.
1) preparing an aqueous stream;
2) preparing a planetary flow;
3) heating the oil phase of step 2) to 60 to 80 ° C; And
4) mixing and stirring the oily type heated at step 3) with the aqueous stream of step 1).
The cosmetic composition for lotion according to claim 5, wherein the oil phase of step 2) is at least one selected from the group consisting of jojoba seed oil, perilla seed oil, evening oil, pumpkin seed oil and olive oil. Way.
1) preparing a planetary flow;
2) adding liquid water, algae and sugar to the oily water of step 1) to prepare a liquid type soap base;
3) aging the liquid type soap base of step 2); And
4) dissolving the aged liquid type soap base of step 3) using a fermentation broth of hot water extract of bracken water as a solvent.
A method for producing a cosmetic composition for a cleanser comprising the same ratio of the hot-water extract of green tea and the hydrolyzed starch-water extract as a solvent in addition to the fermentation liquid of the bracken-hot-water extract in the step 4) of claim 7.
8. The method for producing a cosmetic composition for a cleanser according to claim 7 or 8, wherein the oily oil of step 1) is at least one selected from the group consisting of coconut oil, palm oil, castor oil, olive oil and evening primrose oil.

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