KR20140069766A - Composition for selective anti-bacterial comprising onion extract - Google Patents

Abstract

According to the present invention, disclosed is an anti-bacterial composition comprising an onion extract as an active ingredient which has selectively effective anti-bacterial activity against at least one bacterium among tragomaschalia, dandruff bacillus, acne germs, and dental caries. The composition comprising the onion extract as an active ingredient, according to an aspect of the present invention, can be valuably used as a cosmetic composition since the composition has almost no irritation to the skin.

Description

TECHNICAL FIELD The present invention relates to a selective antimicrobial composition comprising onion extract,

The present invention relates to compositions that selectively antimicrobially act against noxious bacteria.

Acid, dandruff, acne and tooth decay are known to be caused by certain microorganisms. Synthetic chemicals are used to control the causative bacteria of denture, dandruff, acne and cavities, but some synthetic chemicals cause secondary skin irritation and can cause skin allergies when used on sensitive skin Its use in the point is limited. Due to these limitations, consumers are increasingly denied synthetic chemicals, and there is growing interest in skin-safe natural substances that can inhibit the growth of odor, dandruff, acne, and cavities.

Korean Patent Laid-Open No. 10-2011-0012070 (published Feb., 2011)

The present invention is intended to provide a composition having an antibacterial effect selectively effective against at least one of an aerobic bacteria, a dandruff bacteria, an acne bacteria and a carpet bacteria.

One aspect of the present invention provides an antimicrobial composition comprising an onion extract as an active ingredient and having an effective antimicrobial action selectively against at least one of an aerobic bacteria, a dandruff bacterium, an acne bacterium, and a dental carcass.

The composition according to one aspect of the present invention comprises an onion extract as an active ingredient and has an effective antibacterial activity against at least one of an aerobic bacteria, a dandruff bacteria, an acne bacteria and a caviphilus, and at the same time, Skin antibiotics can exhibit selective antimicrobial effects that do not have an effective antimicrobial action. In addition, the composition comprising the onion extract according to one aspect of the present invention as an effective ingredient scarcely gives skin irritation, and thus can be usefully used as a skin external preparation, specifically, a cosmetic composition.

FIG. 1 is a graph showing the degree of skin irritation of onion extracts and conventional preservatives.

As used herein, the term "skin " refers to a tissue covering the body surface of an animal, and is a broad concept including a scalp and hair as well as a tissue covering a body surface such as a face or a body. As used herein, the term " extract " includes substances obtained by extracting the components therein from natural products, and includes all kinds of extraction methods or kinds of extracted components. For example, it is a broad concept including a specific component of a natural product such as a component dissolved in a solvent such as water or an organic solvent or an oil extracted from a natural product.

The composition according to one aspect of the present invention may include an onion extract as an active ingredient to selectively and effectively perform an antibacterial action on at least one of an aerobic bacteria, a dandruff, an acne bacteria and a tooth decay bacteria. The selective antimicrobial action of at least one of bacteria, dandruff, acne bacteria, and cavalry bacteria is to be selected from the group consisting of bacterium, dandruff, acne bacteria and dentifrice other than at least one bacterium, , It means that the antimicrobial action is selectively effective only for at least one bacterium of the aerobic bacteria, stomach bacterium, acne bacterium, and decidua bacteria without effecting effective antibacterial activity to the skin benefit bacteria.

In one aspect of the present invention, " effective antimicrobial activity " means an action that substantially inhibits the activity and proliferation of the bacterium to render it nonfunctional.

The composition according to one aspect of the present invention includes an onion extract as an active ingredient to inhibit biofilm formation of at least one of an aerobic bacteria, a dandruff bacteria, an acne bacteria and a carpet bacterium to produce a biofilm of an aerobic bacteria, a dandruff, The above-mentioned bacteria can be antimicrobially acted. Specifically, the composition according to one aspect of the present invention is substantially not involved in the formation of biofilms of other bacteria, specifically skin-damaging bacteria, more specifically, skin benefit bacteria, except for the odoriferous bacteria, the dandruff bacteria, the acne bacteria and the caries bacteria, It is possible to selectively and effectively perform an antibacterial action against at least one of bacteria, dandruff, acne bacteria and decidua.

As used herein, the term " biofilm " refers to an environment in which a plurality of bacteria coexist in a viscous substance secreted by a germ or the like, and is an important factor involved in fungi colonization and proliferation. When the biofilm is formed, the fixing and propagation of the microorganism becomes active. Therefore, when the formation of the biofilm is inhibited, the microorganisms can be prevented from being fixed and proliferated, thereby realizing an antibacterial effect. Therefore, a substance having excellent biofilm formation inhibiting ability can have an excellent antimicrobial effect or a symptom prevention and treatment effect by the bacterium.

The antimicrobial composition comprising an onion extract according to one aspect of the present invention as an active ingredient comprises a skin hypolipidemic composition which is substantially non-irritating to the skin and thus is highly safe for the skin.

In one aspect of the present invention, the onion ( Allium cepa ) refers to a plant belonging to the lily of the lily family, and the onion according to one aspect of the present invention includes all the onions that can be called onions, do.

In one aspect of the invention, the onion extract may be prepared by conventional methods in the art. In another aspect of the present invention, the onion extract can be prepared by extracting with an organic solvent exemplified by water or an alcohol, followed by filtration and drying.

In one aspect of the present invention, the aerobic bacteria include all of the causative microorganisms, and specifically include micrococcus luteus , Corynebacterium diphtheria , and Corynebacterium xerosis ). ≪ / RTI > In another aspect of the present invention, the dandruce includes all of the bacteria causing dandruff, and specifically includes Malassezia furfur . In another aspect of the present invention, the acne bacteria include all the bacteria causing acne, and specifically include Propionibacterium acnes . In another aspect of the present invention, the caviphilus includes all of the bacteria causing cavities, i.e., dental caries, and specifically includes Streptococcus mutans .

In one aspect of the present invention, the term skin inflammatory bacteria refers to a bacterium existing in normal human skin, specifically, a bacterium which exists in normal human skin and inhibits the proliferation of disease causative microorganisms and has an antibacterial action by itself. More specifically, the skin biocide comprises Staphylococcus epidermidis .

The composition according to one aspect of the present invention comprises from 0.01 to 10% by weight, especially from 0.5 to 5% by weight, more specifically from 0.1 to 4% by weight, more particularly from 0.12 to 3% by weight, of onion extract . ≪ / RTI > When included in the above range, it is appropriate to exhibit the intended effect of the present invention, and it may be appropriate to include the above range in terms of cost effectiveness. Specifically, when the onion extract is contained in the above range, it exhibits an excellent antibacterial action against at least one of an aerobic bacteria, a dandruff, an acne bacteria and a tooth decay, and at the same time, .

One aspect of the present invention provides an antibacterial external preparation for skin comprising an onion extract as an active ingredient and having an antibacterial effect selectively effective against at least one of an aerobic bacteria, a dandruff bacteria, an acne bacteria and a tooth decay bacteria. One aspect of the present invention provides an antimicrobial cosmetic, food and pharmaceutical composition comprising an onion extract as an active ingredient and having an effective antibacterial activity selectively for at least one of an aerobic bacteria, a dandruff bacteria, an acne bacteria and a dental carcass.

The cosmetic composition according to one aspect of the present invention may be provided in all formulations suitable for topical application. For example, it may be provided as a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol. Such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to one aspect of the present invention may contain other ingredients which can give synergy to the main effect, preferably to the extent that the main effect is not impaired. The cosmetic composition according to one aspect of the present invention may further include a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antioxidant, a pH adjuster, an organic or inorganic pigment, a flavoring agent, a cold agent, or an antiperspirant agent . The blending amount of the above components can be easily selected by those skilled in the art within the range not impairing the objects and effects of the present invention. The blending amount thereof is 0.01 to 5% by weight, specifically 0.01 to 3% by weight .

The formulation of the food composition according to one aspect of the present invention is not particularly limited, but can be formulated into, for example, tablets, granules, powders, liquids, solid preparations and the like. Each formulation may be blended without difficulty by a person skilled in the art according to the purpose of formulation or use, in addition to the active ingredient, and the synergistic effect may occur when the composition is applied simultaneously with other ingredients.

Determination of the dosage of the active ingredient is within the level of ordinary skill in the art and its daily dose is, for example, 1 μg / kg / day to 10 mg / kg / day, more specifically 10 μg / kg / day Day to 100 mg / kg / day, but it is not limited thereto, and it may be various factors such as the age, health condition, and complication of the subject to be administered ≪ / RTI >

The pharmaceutical composition according to one aspect of the present invention can be administered orally, rectally, transdermally, intravenously, intramuscularly, intraperitoneally, intramuscularly, intradermally or subcutaneously.

Formulations for oral administration may be, but are not limited to, tablets, pills, soft or hard capsules, granules, powders, solutions or emulsions. Formulations for parenteral administration may be, but are not limited to, injections, drops, lozenges, ointments, gels, creams, suspensions, emulsions, suppositories, patches or spraying agents.

The pharmaceutical composition according to one aspect of the present invention may contain additives such as a diluent, an excipient, a lubricant, a binder, a disintegrant, a buffer, a dispersant, a surfactant, a colorant, a fragrance or a sweetener as necessary. The pharmaceutical composition according to one aspect of the present invention can be prepared by a conventional method in the art.

The effective ingredients of the pharmaceutical composition according to one aspect of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 [mu] g / kg / day to 1 g / kg / day, Kg / day, more specifically, 10 μg / kg / day to 1 mg / kg / day, and more particularly 50 μg / kg / day to 100 μg / kg / day. The pharmaceutical composition according to one aspect of the present invention may be administered once to three times a day, but is not limited thereto.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples and test examples. However, these examples and test examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

[Example] Preparation of onion extract

10 times as much weight of onion is added to chopped onion, and the mixture is heated at 80 to 90 ° C for more than 2 hours and concentrated. The concentrate is filtered through a filter paper using water as a solvent. It is then dried in the shade and made into powder, which is used as the onion extract of the experiment below.

[Test Example 1] Evaluation of antimicrobial activity

The minimum inhibitory concentration (MIC) of onion extracts of each onion was evaluated in order to investigate the antimicrobial activity of onion extracts against odoriferous, dandruce, acne and decidua.

First, the test bacterium and the culture medium were prepared as shown in the following table, and the test bacterium was prepared by culturing each test bacterium according to each condition in the corresponding medium.

Test bacteria badge Culture conditions Smell M. luteus
(ATCC 10240) Brain heart extract
(Brain heart infusion, BHI) 32 ℃, 1 day shaking culture C. diphtheria
(ATCC 11951) Brain Heart Leakage (BHI) 32 ℃, 1 day shaking culture C. xerosis
(ATCC 771) Brain Heart Leak (BHI) + 10% serum 32 ℃, 1 day shaking culture Cavities S. mutans
(KCTC 3065) Brain Heart Leakage (BHI) 32 ℃, 1 day shaking culture Acne bacteria P. acnes
(ATCC 6919) Brain Heart Leakage (BHI) Incubation for 2 days at 25 ° C Dandruff M. furfur
(ATCC 12087) Ovale Incubation at 32 캜 for 3 days

The test samples were prepared by mixing onion extracts at a concentration of 4% in the medium of each test organism. 200 쨉 l of the test sample prepared in row 1 of the 96-well plate was added. In the remaining wells, 100 μl of the medium corresponding to each strain was added. 2-fold serial dilution was performed from the first row to the last row. 10 μl of each test solution was added to all wells to give 10 ⁵ CFU / ml. After re-culturing under the culture conditions of the above test bacteria, the minimum concentration of the test sample without the growth of bacteria was determined as the MIC value by judging whether or not each test organism was proliferated based on the degree of suspension relative to the untreated group. When the mixture was opaque and it was difficult to judge the growth of bacteria, it was confirmed by microscopic observation. The results are shown in the table below.

Test bacteria MIC of onion extract Smell M. luteus One% C. diphtheria One% C. xerosis One% Cavities S. mutans 4% Acne bacteria P. acnes 0.5% Dandruff M. furfur 4%

From the above results, it can be confirmed that the onion extract has antimicrobial effect against the odorant, the deciduous germ, the acne germ, and the dandruff germ.

[Test Example 2] Biofilm formation inhibiting ability evaluation

Propionibacterium acnes (ATCC 6919), known as an acne bacterium, and Staphylococcus epidermidis (ATCC 35984), which is known to exhibit antibacterial activity against skin noxious bacteria, The biofilm inhibitory effect of the extract was evaluated.

Propionibacterium acnes were anaerobically cultured in BHI medium for 48 hours and staphylococcus epidermidis were cultured in TSB medium for 24 to 48 hours to be in an exponential phase, To achieve 10 ⁸ CFU / ml. 200 μl of a 2% diluted onion extract sample was added to each well of the 96-well plate on the first line, and 100 μl of the medium was added to the remaining wells. The mixed solution of line 1 was well mixed, and then 100 μl of the mixture was added to the second line. After mixing well, 100 μl of the mixture was added to the third line and diluted 1/2. The diluted test wells were inoculated in a volume of about 10% to a final concentration of 10 ⁶ CFU / ml, and the inoculated 96-well plate was incubated for 48 hours at 32 ° C. The culture was removed and each well was washed twice under mild conditions to allow 100 μl of DI water to flow through the wall of the well. 100 [mu] l of 0.1% crystal violet was added and stained for 5 to 10 minutes. Thereafter, 200 [mu] l of purified water was again washed twice under mild condition so that it flows on the wall surface of each well. 100 ㎕ of 99% ethanol was added and the dye was extracted by shaking at 85-90 rpm for 5 minutes. The absorbance was then measured at 590 nm. SpectraMax 190 / Molecular devices were used as absorbance measurement devices. The wells that were not inoculated with the negative control (0%) and the wells inoculated with the standard bacteria were subjected to positive control (100%), and OD ₅₉₀ values measured in each test organism were extrapolated to bio Film formability was calculated. The results are shown in the table below.

[Equation 1]

Concentration of Anma extract (%) P. acnes S. epidermidis 2 31.754 27.140 One 37.136 29.665 0.5 52.270 22.702 0.25 56,000 86.259 0.125 55.934 115.807 0.0625 100.296 123.826 0.03125 96.678 114.550 0.015625 88.619 117.587

When the biofilm forming ability was more than 70% as compared with the positive control, it can be judged that a biofilm was formed. As a result, the biofilm formation was suppressed by the 0.125% onion extract of P. bacterium acnes, Staphylococcus epidermidis, an over-the-top skin, was inhibited only by 0.5% onion extract. As can be seen from the above results, when the onion extract of proper concentration is applied to the skin, it inhibits biofilm formation for acne bacteria and does not affect the skin uptake bacteria.

[Test Example 3] Evaluation of skin irritation

The skin irritation of onion extracts and preservatives, which are mainly used in cosmetics, was evaluated. 20 μl of each test substance was placed on Haye's Chamber and applied to 30 subjects. The patches were removed after 24 hours. After 30 minutes and 24 hours after the removal of the patch, skin conditions were visually observed and the degree of skin irritation based on the following table, which was modified based on the CTFA guidelines (1981) and Frosch & Kligman (1979) I gave a score for.

score Explanation 0 no response One Weak erythema or rash 2 Moderate erythema 3 Strong erythema with edema 4 Strong erythema with edema and blisters

The results obtained by combining the scores are shown in the following table and FIG.

Test substance density(%) menstruum score Judgment Ethyl paraben 0.1 50% ethanol 2.82 Light stimulus Ethyl paraben 2.55 50% ethanol 2.02 Light stimulus 1,2-hexanediol 20 Purified water 3.21 Moderate irritation Octanediol 1.25 50% ethanol 2.42 Light stimulus Octanediol 1.88 50% ethanol 2.82 Light stimulus MCI / MI 30 water 3.33 River stimulation 1,2-pentylene glycol 25 Purified water 7.69 River stimulation 1,2-pentylene glycol 10 Purified water 2.24 Light stimulus 1,2-pentylene glycol 5 Purified water 2.24 Light stimulus Onion extract 0.5 Purified water 0.66 No stimulation

* MCI / MI is methylchloroisothiazolinone and methylisothiazolinone.

As shown above, the onion extract had a lower skin irritation level than the preservatives used in conventional cosmetic compositions at a concentration showing antibacterial effect. This means that the onion extract has excellent selective antimicrobial effect and gives almost no irritation to the skin, so that it is excellent in safety as a cosmetic composition component.

Claims (9)

Onion extract as an active ingredient,
A composition for antimicrobial use which selectively inhibits the antibacterial action against at least one bacterium of the genus Lactobacillus, Staphylococcus aureus, Acne bacterium and Quercus bacterium.
The method according to claim 1,
The composition comprises at least one of Micrococcus luteus , Corynebacterium diphtheria , and Corynebacterium xerosis .
The method according to claim 1,
Wherein the dandruff comprises Malassezia furfur .
The method according to claim 1,
Wherein the acne bacterium comprises Propionibacterium acnes .
The method according to claim 1,
The composition according to claim 1, wherein the composition comprises Streptococcus mutans .
The method according to claim 1,
Wherein the composition does not have an effective antimicrobial action on the skin biocide.
The method according to claim 6,
Wherein the dermatophyte comprises Staphylococcus epidermidis .
The method according to claim 1,
Wherein the composition inhibits the biofilm of at least one of an aerobic bacteria, a stomach bacteria, an acne bacteria and a carpet bacterium.
The method according to claim 1,
Wherein the composition is a cosmetic composition.

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