JP2007001948A - Deodorization-active agent and skin care preparation for deodorization - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an effective deodorization-active agent that is effective for deodorizing body odor, particularly armpit odor, and does not exert large influences on indigenous skin bacteria flora. <P>SOLUTION: The deodorization-active agent comprises extracts obtained by solvent extraction from buds of Hemerocallis fluva Linne and exhibits bactericidal effects selectively on only bacteria belonging to genus Corynebacterium, which are bacteria causing armpit odor. The skin care preparation for deodorization comprises the deodorization-active agent. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  TECHNICAL FIELD The present invention relates to a novel use as a deodorant active agent for a licorice flower extract and a composition for external use for deodorization.

  The licorice (Hemerocallis fluva Linn.) Is a lily family plant native to China and is widely cultivated in various parts of China. In the Greater China region, the dried buds are boiled and dried, and are commonly used as general-purpose foods. In addition, the flower buds are said to have antipyretic effects, and these are decocted and taken. In addition, the floret or its extract is not applied to the skin and is not used for deodorization or sterilization purposes.

  By the way, the odor which is a unique odor among human body odors is generated when apocrine sweat secreted from the apocrine sweat glands in the axilla is decomposed by the skin surface flora. Corynebacterium zerosis, one of its skin surface flora, is a causative bacterium that produces a particularly strong and unpleasant odor. Therefore, for the purpose of suppressing the generation of the odor, conventionally, an aluminum salt such as hydroxyaluminum chloride (aluminum chloride hydrate) has been used as an antiperspirant astringent. In addition, antibacterial agents having a bactericidal activity such as benzalkonium chloride are widely used for suppressing causative bacteria (see Non-Patent Document 1). However, the antibacterial agent acts not only on the odor-causing bacteria, but also on other bacterial species that form the skin flora, so the risk of damaging the self-cleaning action inherent to the skin due to the formation of a healthy skin flora Had sex.

Fukubayashi Tomoko “Self-resident bacteria and cosmetics” Fragrance Journal, March 2003, p. 23-28

  In the above circumstances, there has been a demand for a deodorizing active agent that does not greatly affect the skin flora and shows a bactericidal effect only on the odor-causing bacteria.

  As a result of diligent research in view of the above circumstances, the present inventor has found that an extract obtained from a readily available licorice flower edible that is edible for general use has a selective bactericidal effect against Corynebacterium spp. The present invention has been completed. That is, the present invention is a deodorant active agent comprising an extract obtained by solvent extraction from licorice florets, and the second invention is water, a water-soluble organic solvent or a solvent used for the solvent extraction. The deodorizing active agent is selected from mixed solvents. The third invention is the deodorant active agent characterized in that the deodorization is aimed at deodorizing the odor, and the fourth invention is a selection against Corynebacterium which is the odor-causing bacterium. The deodorant active agent is characterized by being based on a sterilizing action.

  Furthermore, the fifth invention of the present application lies in a deodorizing skin external composition characterized by blending the above deodorizing active agent. Corynebacterium sp., Which is the target of the antibacterial activity of the present invention, is not particularly limited as long as it is a gram-positive gonococcus belonging to the genus of the genus, but is preferably a bacterium that is the main cause of odor generation. Examples of such bacteria include Corynebacterium minutissimum, Corynebacterium xerosis, Corynebacterium tenuis, and the like, and particularly preferably Corynebacterium xerosis.

  The licorice florets used in the present invention are also used for food, and the extract obtained therefrom has no problem in safety and is excellent in stability. The extract obtained by solvent extraction from Honkan flower buds has a selective bactericidal effect against Corynebacterium spp. It has the effect. The deodorant active agent of the present application has a selective bactericidal effect, so it does not affect the flora of the skin surface other than the target fungus, and does not impair the self-cleaning action inherent to the skin due to the formation of healthy skin flora .

  The licorice florets used in the present invention are called gold needle vegetables and dried lily bud as described above, and are distributed as general-purpose foods, and these can be used. In the present invention, the extraction solvent used for solvent extraction can be any known extraction solvent used for solvent extraction from plant materials. When a solvent having a relatively high hydrophilicity is used as the extraction solvent, an effective extract that meets the object of the present invention can be obtained. As such a solvent, a solvent selected from water, a water-soluble organic solvent or a mixed solvent thereof is particularly preferably used in the present invention. The water-soluble organic solvent specifically includes lower alcohols such as methanol, ethanol, propanol, isopropanol and butanol, polyhydric alcohols such as butylene glycol and propylene glycol, and fatty acid carboxylic acids such as methyl acetate and ethyl acetate. Examples include esters and other highly polar organic solvents such as acetone. A preferable example of the extraction solvent is an aqueous ethanol solution.

  In the extraction, first, the licorice florets are cut and pulverized by a known appropriate means. Extraction with a solvent may be performed according to a known method. For example, when water, a water-soluble organic solvent, or a mixed solvent thereof is used as an extraction solvent, the extraction solvent amount is 1 to 50 times, preferably 3 to 10 times, 0 to 100 ° C. And extraction under a temperature condition lower than the boiling point of the extraction solvent for 0.1 to 50 hours, preferably 0.5 to 24 hours. Although the extraction may be in a stationary state, it is desirable to perform the extraction with moderate stirring for more efficient extraction.

  The licorice flower bud extract thus obtained can be applied as a deodorant active agent as it is, but may be concentrated or dried as necessary. Further, the extract may be re-extracted with another solvent or the like, or a fraction obtained by fractionation and purification by a known fractionation means or purification means may be used.

  The deodorizing active agent of the present invention can be widely applied as a deodorizing active agent in external use for skin, clothing, living environment and the like. In particular, it is particularly useful as a deodorizing active agent for the bad smell caused by Corynebacterium spp. Which is the target of the antibacterial activity of the extract of the present invention, for use in external use on the skin.

  The deodorant active agent of the present invention can be applied as a deodorizing skin external composition for the purpose of deodorizing, particularly deodorizing odor, by blending into the composition for external skin as a deodorizing active ingredient. The blending amount of the licorice flower extract as a deodorant active agent in the deodorant skin external composition according to the present invention is not particularly limited, and is appropriately determined depending on the extraction method of the extract, the method of application to the skin, the applied dosage form, etc. Since it is determined, it is not something that can be specified in general, but generally, based on the total amount of the composition for external use, the licorice floret extract is 0.00001 to 15.0 mass% in terms of dry matter, It is preferable to mix | blend especially in the range of 0.0001-5.0 mass%. If it is this range, a suitable deodorizing effect will be exhibited.

Examples of the deodorizing skin external composition containing the licorice flower extract of the present invention include various pharmaceuticals, quasi-drugs, cosmetics and the like. The dosage form is not limited and can be various. Specifically, ointment, lotion, emulsion, cream, pack, granule, spray, aerosol, face wash, roll-on agent, and extract of the present application were blended. It can be set as the sheet agent, patch, or haptic agent which impregnated the sheet-like base material with the composition. The cosmetic is not particularly limited as long as it is applied to human skin, and various skin cosmetics and foundations, makeup cosmetics, hair cosmetics such as shampoos and rinses, treatments, bathing agents, facial cleansers and the like. Can be.

  Moreover, the composition for external use for deodorizing skin of this invention can be mix | blended combining the various components normally used in the range which does not impair the effect as needed. For example, oils, surfactants, humectants, thickeners, antioxidants, UV absorbers, UV scattering agents, antiseptics, fragrances, dyes, pigments and the like.

  EXAMPLES Hereinafter, although this invention is explained in full detail based on an Example, this invention is not limited by these. In addition, all% in an Example means the mass%.

Example 1 (Production of Honkan elephant flower bud extract)
The dried lily buds dried with boiled water were cut with a knife, and 5 times by weight 50% ethanol aqueous solution was added thereto, and the mixture was stirred and extracted at room temperature for 24 hours. The extraction residue was removed by filtration to obtain a licorice flower extract (solid content: 13.51%).

Example 2 (Antimicrobial activity against various bacterial species)
Using the extract produced in Example 1, growth inhibition against the following various bacteria was examined. Corynebacterium xerosis, Staphylococcus aureus, Staphylococcus epidermidis, Eschirichia coli, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Propionibacterium acnes ), Malassezia furfur.

  Specifically, the optimal agar medium of each bacterium was dissolved, and each bacterium was added immediately before the agar medium solidified, and solidified on a plate dish. A sterilized filter paper disk having a diameter of 8 mm was placed on the solidified plate medium, and 10 μL of the licorice flower bud extract obtained in Example 1 was dropped therein and gradually diffused into the medium through the filter paper. The bacteria were cultured under optimum culture conditions, and the presence or absence of the antibacterial action was confirmed based on whether or not a inhibition circle was formed by inhibiting the growth of the bacteria in the plate medium. 50% ethanol was used as a control. The results are shown in Table 1.

[Table 1]
――――――――――――――――――――――――――――――――
Presence of blocking circle
―――――――――――――――
Extract of this application 50% ethanol ――――――――――――――――――――――――――――――――
C.xerosis ＋ −
S.aureus (Staphylococcus aureus) − −
S. epidermidis--
E.coli--
P.aeruginosa (Pseudomonas aeruginosa) − −
B. subtilis − −
P. acnes--
M.furfur (malassezia)--
――――――――――――――――――――――――――――――――

  As is apparent from Table 1, the licorice floret extract of the present application has a unique property of selectively exerting antibacterial activity only against the odor-causing bacterium Corynebacterium xerosis.

Example 3 (Odor odor prevention test)
A roll-on agent containing a licorice flower bud extract having the following composition was prepared, and a deodorization test for odor was conducted according to the following evaluation method.

[Roll-on agent composition]
Compounding ingredients Compounding amount (%)
(1) Honkan elephant flower bud extract of Example 1 5.0
(2) Citric acid 0.5
(3) Sodium citrate 0.5
(4) Ethanol 40.0
(5) Residual amount of purified water

[Evaluation methods]
Using 10 male subjects who were aware of the odor, the above-described roll-on agent or a placebo containing no Honkan elephant flower bud extract was applied to the armpit and subjected to sensory evaluation regarding the odor. Compared to the case of no application, the effect of improving odor was evaluated according to the following criteria.

Evaluation criteria 3: Effective 2: Slightly effective 1: Slightly effective 0: No effect

  The results of sensory evaluation are shown in Table 2. In addition, a result is shown with the average value of 10 evaluation points. It can be seen that the licorice flower extract of the present application has a high deodorizing effect against the odor.

[Table 2]
――――――――――――――――――――――
Elapsed time after use (minutes)
――――――――――
60 120
――――――――――――――――――――――
Honkan elephant flower extract 2.8 2.7
Combined roll-on placebo 1.4 0.2
――――――――――――――――――――――

  The deodorant active agent comprising the licorice floret extract of the present application can be applied as a deodorant active ingredient to various skin deodorizing compositions. Although the example of a prescription is shown below, it is not necessarily limited to these.

Example 4 (skin lotion)
Compounding ingredients Compounding amount (%)
(1) Honkan elephant flower bud extract of Example 1 5.0
(2) POE monooleate (20 EO) 0.5
(3) Propylene glycol 1.0
(4) Glycerin 2.0
(5) Methylparaben 0.05
(6) Ethanol 7.0
(7) Fragrance proper amount
(8) Purified water balance

Example 5 (milky lotion)
Compounding ingredients Compounding amount (%)
(1) Stearic acid 1.5
(2) Cetyl alcohol 1.0
(3) Vaseline 4.0
(4) Liquid paraffin 8.0
(5) POE monooleate (20 EO) 2.0
(6) Triethanolamine 1.0
(7) Honkan elephant flower bud extract of Example 1 5.0
(8) Fragrance proper amount
(9) Residual amount of purified water

Example 6 (cream)
Compounding ingredients Compounding amount (%)
(1) Stearic acid 7.0
(2) Cetyl alcohol 3.0
(3) Beeswax 2.0
(4) Olive oil 15.0
(5) POE sorbitan 2.0
Monooleate (20 EO)
(6) Honkan elephant flower bud extract of Example 1 5.0
(7) Residual amount of purified water

The deodorant active agent of the present invention does not greatly affect the normal flora of the epidermis and acts selectively on the genus Corynebacterium causing the odor, so that the odor caused by the genus Corynebacterium, for example, It can be used as various types of skin deodorizing compositions for clothes, odor preventing compositions for clothing, or deodorizing compositions for living environment for the purpose of deodorizing odors and the like.

Claims (5)

A deodorizing active agent comprising an extract obtained by solvent extraction from a flower of Honkan elephant. The deodorant active agent according to claim 1, wherein the solvent used for the solvent extraction is selected from water, a water-soluble organic solvent, or a mixed solvent thereof. The deodorant active agent according to claim 1 or 2, characterized in that the deodorization aims at deodorizing a bad odor. The deodorant active agent according to claim 3, wherein the deodorant activity is based on a selective bactericidal action against Corynebacterium which is a odor-causing bacterium. A deodorizing skin external composition comprising the deodorant active agent according to any one of claims 1 to 4.