KR20140013848A - Cosmetic compositions for anti-irritants - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating pains, and more specifically, to a cosmetic composition for alleviating pains, wherein the composition lowers the stilumus of the skin, prevents stimulus due to the application of cosmetic products, and has effective anti-inflammatory performance.

Description

Cosmetic composition for stimulation relaxation {Cosmetic Compositions for Anti-Irritants}

The present invention relates to a cosmetic composition for alleviating irritation, and more particularly, to reducing the irritation of skin that has already been irritated, to prevent irritation that may be caused by application of cosmetic products, and to an anti-inflammatory cosmetic composition effective for anti-inflammatory. It is about.

Some ingredients used in topical products are potentially irritating, especially for those with sensitive skin. As an example, hydroxy acids (HAs) and retinoids have been demonstrated to provide cosmetic benefits such as improvements in appearance, skin gloss, aging point treatment, etc. of photodamaged or naturally aged skin. Unfortunately, their high concentrations can sometimes be associated with skin irritation such as skin redness and stinging sensations upon application. Such irritation can be alleviated by lowering the amount of active ingredient in the composition or by reducing the penetration of the active agent through the skin. A serious disadvantage of this approach is that the efficacy is compromised.

HA related irritation can be reduced by increasing the pH of the composition, but this method decreases efficacy with a decrease in HA penetration through the skin. It is desirable to reduce or eliminate stimulation potential while maintaining the efficacy of HA and / or retinoids.

The appearance, frequency, and appearance of stimuli often vary from user to user. The severity of the stimulus for sensitive users can vary from mild to severe. Common symptoms of irritation include itching (pruritus), stinging, burning, tension, tingling, erythema (redness), or edema (swelling).

Environmental conditions such as sunlight, wind, cold and dry air can cause irritation or worsening. In addition, the permeability and sensitivity of the skin to topically applied chemicals that soaps, cleaners, cleaning products, shaving creams, alcohols and other products that remove protective lipids and / or secretions from the skin may not produce irritation. Can be increased.

Similarly, the skin may be irritated by infection, shaving scratches, repeated or excessive washing or bathing, sun exposure, or other mechanical scratches or damage.

In addition to the chemical and environmental causes of skin irritation, many people have an innate sensitivity or genetic predisposition to skin irritants (unique skin irritation). Whatever the exact cause of the irritation, there have been many attempts to reduce the irritation potential of topical products by identifying chemicals that tend to cause irritation and reducing or eliminating their concentration from the product.

Unfortunately, it is often not possible or practical to identify or eliminate all of the irritating chemicals, especially when the irritating chemicals are the active ingredients of the product or are needed for formulation, preservative or other functional reasons.

Accordingly, there is a need for compositions and methods to prevent or reduce skin irritation.

 The present invention provides a cosmetic composition that lowers the irritation of already irritated skin and prevents irritation that may be caused by application of a cosmetic product.

The present invention can include the following means in order to solve the above-described problems.

Stimulation relief cosmetic composition according to the present invention contains brown hemp extract as an active ingredient.

The brown hemp extract is characterized in that it is additionally extracted with ethyl acetate after extraction by ethanol.

The ethanol is characterized in that the volume ratio of distilled water: ethanol is 1: 1: mixed.

The ethyl acetate is characterized in that the volume ratio of distilled water: ethyl acetate is composed of 1: 1 mixed.

The extract is characterized in that formed in 0.1 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition according to another embodiment of the present invention contains a brown hemp extract as an active ingredient and has a useful function in anti-inflammatory.

The cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing It is characterized by selecting any one formulation selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow.

 The present invention lowers the irritation of already irritated skin, prevents irritation that may be caused by application of cosmetic products, and provides an effective effect on anti-inflammatory.

1 to 5 are graphs showing the beneficial effect of brown hemp extract extracts on anti-inflammatory.

The present invention relates to a cosmetic composition for skin irritation relief containing brown hemp extract as an active ingredient.

The brown hemp (Cladophora wrightiana Harvey) is a seaweed with seaweed (海 (), the height is 20 ~ 40cm, the largest among the hemp. The body branches in a thin line. It is blueish green when alive, but turns brown when dying. It is distributed in Busan, Geomun-do, Jeju-do, and Japan. However, almost no research on brown hemp is known as its efficacy is not known.

Brown hemp extract according to the present invention can be prepared as follows.

Wash the brown hemp with water and dry it in the shade. The brown hemp can be placed in an extraction container and extracted for a predetermined time at an appropriate temperature in an appropriate amount of the extraction solvent.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used. Preferably, the brown hemp is extracted with ethanol extract extracted with ethanol again with ethyl acetate. It is appropriate.

This is because the ethanol extract has a large amount of salt components remaining in itself, so that the ethanol extract can be extracted again with ethyl acetate to remove the salt component, so that the algae can exhibit its unique activity. Existing ethanol extraction method has been solved by two extraction methods that are difficult to remove the salt components contained in seaweed.

On the other hand, the ethanol can be used in a variety of concentrations, preferably made of 60 to 90% ethanol, more preferably 80% ethanol (concentration when mixing 20 ml of ethanol in a proportion of 60 ml of water).

In addition, the ethyl acetate may be used in various concentrations, but preferably 30 to 60% ethyl acetate, more preferably 50% ethyl acetate (concentration when mixing 300 ml of ethyl acetate in a ratio of 300 ml of water). Suitable.

The extraction is preferably made for 50 to 100 hours at 15 ~ 35 ℃.

The brown hemp extract in the cosmetic composition of the present invention may be contained in an amount of 0.1 to 20% by weight based on the total weight of the composition.

The composition of the present invention may contain one or more active ingredients exhibiting the same or similar function in addition to the brown hemp extract.

Cosmetics made of a cosmetic composition containing the brown hemp extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of emulsified form include nutrition lotion, cream, essence, etc., and cosmetics of solubilized form have softening longevity. In addition to cosmetics containing the mixed extract of the present invention, by containing a dermatologically acceptable medium or base, it can be prepared in the form of adjuvants that can be applied topically or systemically as commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the brown hemp extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological field, such as any other ingredients commonly used. And, the above ingredients can be introduced in amounts commonly used in the field of dermatology.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred embodiments and experimental examples are provided to facilitate understanding of the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Example 1 Preparation of Extract

Wash the brown hemp with water and dry it in the shade. The brown brown hemp extract was crushed finely in a crusher and 80% ethanol was added to extract the brown hemp extract. 3 g of the extract was added to 300 ml of distilled water and 300 ml of ethyl acetate, and extracted at room temperature for 72 hours to prepare a brown hemp fraction.

Experimental Example  One- NO  Production inhibitory effect

After treatment with fractions of Example 1 (25, 50 and 100 μg / mL) and LPS (1 μg / mL) in RAW 264.7 cells for 24 hours, the cell viability was confirmed using LDH analysis. It was confirmed that there was no cytotoxicity at the treatment concentrations of the horse fraction and extract, and then the NO production inhibitory activity was measured and the results are shown in FIG. 1. Referring to Figure 1, it was found that the brown hemp extract extract significantly inhibits the production of NO.

Experimental Example  2: prostaglandin E2 ( PGE ₂ ) Inhibitory effect

In order to investigate the effect of the extract of Example 1 on the production of prostaglandin E2, the mouse macrophage line RAW264.7 cells were dispensed 5 × 10 ⁴ cells in 96-well plate medium containing 10% fetal calf serum Incubated for 24 hours at. Aspirin was then added to a final concentration of 500 μM to inhibit the activity of prostaglandin biosynthetic enzyme (prostaglandin H2 synthetase, or cyclooxygenase) remaining in the cells. Two hours after aspirin treatment, each well containing RAW264.7 cells was washed twice with PBS, and 200 μl of 10% FBS-DMEM medium containing 1 μg / ml LPS (lipopolysaccharide) was added to each well (control). In medium without LPS). Then, the extract of the preparation was treated to a final concentration of 1% and then incubated for 16 hours. Prostaglandin inhibitory effect of the extract was determined by taking an appropriate amount of the culture supernatant and quantifying PGE2 biosynthesized for 16 hours. The amount of PGE ₂ was measured and quantified in an ELISA reader 450 nm, the results are shown in FIG.

As shown in FIG. 2, the brown hemp extract showed a very good inhibitory effect on prostaglandin E2 production. This suggests that brown hemp extract can be a very useful ingredient for anti-inflammatory.

Experimental Example  3: TNF -α, IL -6, IL -1β

      Expression of TNF-α, IL-6 and IL-1β after 24 hours of incubation with the fraction of Example 1 (25, 50 and 100 μg / mL) and LPS (1 μg / mL) in RAW 264.7 cells The results are shown in FIGS. 3 to 5.

As shown in FIGS. 3 to 5, brown hemp horse extract may be inferred from the concentration-dependent inhibition of expression of TNF-α, IL-6, and IL-1β, which may be a very useful component for anti-inflammatory.

[ Example  2 and Comparative Example ]

To prepare a cosmetic containing the brown hemp extract of Example 1, and to compare with the cosmetic composition lacking the extract was prepared in the composition ratio of Table 1 below.

division Content (% by weight) ingredient Example 2 Comparative Example Brown hemp extract 4.0 - Cetearyl octanoate 5.0 5.0 Isopropyl palmitate 5.0 5.0 Polyacrylamide / C13-14 Isoparaffin / Laureth-7 2.0 2.0 Polysorbate 60 1.7 1.7 Solbitan Sesquioleate 1.3 1.3 Spices, preservatives dose dose Purified water Balance Balance Sum 100 100

Experimental Example  4: human skin Patch test

      In the past, the skin irritation effect was confirmed in human skin patch test of 20 women in their 20s and 40s who had no hypersensitivity reaction to skin irritation and have no skin disease or skin allergy symptoms. Example 2 and Comparative Example, in which 5.0% of lactic acid, a skin irritant, were added as an irritant, were used. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was added dropwise into a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then airtightly wrapped in the forearm inner part of the test subject. The test site was marked with a marking pen after patching for 24 hours and removing the patch. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) and the results are shown in the table.

* Evaluation criteria and score of skin reaction

Symbol score  Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One The border is clear but weak erythema, edema and palsy ++ 2 Clear erythema, papules and small follicles +++ 3 Severe erythema and alveolar, scleroderma

[Equation 1]

Mean response rate = (score × number of responses × 100 × 1/2) / [3 (maximum score) × total number of subjects (ｎ)]

Test substance After 1 hour After 24 hours Reactivity (%)
(n = 20) ± + ++ ± + ++ Example + 5% Lactic Acid
Comparative Example + 5% Lactic Acid 6--
12 2- One - -
2 1- 2.95
8.30

As can be seen from Table 3, it was found that the Example containing the brown hemp extract of the present invention is superior to the skin irritation-reducing effect induced by lactic acid compared to the Comparative Example.

       Based on the results of the previous experimental example, to present a cosmetic composition containing the brown hemp extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Flexible Lotion

Formulation examples of the lotion containing brown hemp extract were prepared as shown in Table 4 below.

Raw material Content (% by weight) Brown hemp extract 1.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

Formulation Example 2 Nutrition Cream

Formulation examples of nutrition cream containing brown hemp extract were prepared as shown in Table 5 below.

Raw material Content (% by weight) Brown hemp extract 4.0 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

Claims (7)

A cosmetic composition for stimulating relaxation comprising brown hemp extract as an active ingredient. According to claim 1, wherein the brown hemp extract is,
After the extraction with ethanol, the cosmetic composition for stimulating alleviation, characterized in that additional extraction with ethyl acetate. The method of claim 2, wherein the ethanol,
A cosmetic composition for alleviating stimulation, characterized in that the volume ratio of distilled water: ethanol is 1: 4 mixed. The method of claim 3, wherein the ethyl acetate,
A cosmetic composition for alleviating stimulation, characterized in that the volume ratio of distilled water: ethyl acetate is mixed at a ratio of 1: 1. The cosmetic composition according to claim 1, wherein the extract is formed in an amount of 0.1 to 20% by weight based on the total weight of the cosmetic composition. Cosmetic composition having a useful function for anti-inflammatory by containing brown hemp extract as an active ingredient. According to claim 1 or 6, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, essence, Cosmetics characterized in that the formulation is selected from the group consisting of nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow Composition.

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